DACHB cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DACHB
Entrez Gene ID	192122
Full Name	dachshund b
Synonyms	zgc:136530
General protein information	Preferred Names dachshund b Names dachshund b
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009305776.2	XP_009304051.1	dachshund b isoform X1
XM_009305776.3	XP_009304051.1	dachshund b isoform X1
XM_021477542.1	XP_021333217.1	dachshund b isoform X2
NM_152956.2	NP_694488.2	dachshund b
XM_017356588.1	XP_017212077.1	dachshund b isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Exome sequencing and functional analyses suggest that SIX6 is a gene involved in an altered proliferation-differentiation balance early in life and optic nerve degeneration at old age.
Iglesias AI, Springelkamp H, van der Linde H, Severijnen LA, Amin N, Oostra B, Kockx CE, van den Hout MC, van Ijcken WF, Hofman A, Uitterlinden AG, Verdijk RM, Klaver CC, Willemsen R, van Duijn CM
Human molecular genetics23(5)1320-32(2014 Mar)

Dachshund homologues play a conserved role in islet cell development.
Kalousova A, Mavropoulos A, Adams BA, Nekrep N, Li Z, Krauss S, Stainier DY, German MS
Developmental biology348(2)143-52(2010 Dec)

The transcription factor six1 inhibits neuronal and promotes hair cell fate in the developing zebrafish (Danio rerio) inner ear.
Bricaud O, Collazo A
The Journal of neuroscience : the official journal of the Society for Neuroscience26(41)10438-51(2006 Oct)

Isolation of three zebrafish dachshund homologues and their expression in sensory organs, the central nervous system and pectoral fin buds.
Hammond KL, Hill RE, Whitfield TT, Currie PD
Mechanisms of development112(1-2)183-9(2002 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DACHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DACHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa147194	XM_021477542.1 Latest version!	Danio rerio dachshund b (dachb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa53216	NM_152956.2 Latest version!	Danio rerio dachshund b (dachb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa39553	XM_009305776.3 Latest version!	Danio rerio dachshund b (dachb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa53669	XM_017356588.1 Latest version!	Danio rerio dachshund b (dachb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa39553	XM_009305776.2	Danio rerio dachshund b (dachb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DACHB ( XM_021477542.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021333217.1 Danio rerio dachshund b (dachb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa147194
Clone ID Related Accession (Same CDS sequence)	XM_021477542.1
Accession Version	XM_021477542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	dachshund b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAG CTTTAAAGAT ACAGAAGATG AAGATGATGA TGAACCTGCA CAAGACTCAC 
AATGGCTCCG AATTCGATTC AGATGAGCTG AACTCTAACG CCGGTACAGT ATGCAGCACC 
CTGTCCTGGG AGAGAGAGAA ACATTCATCA CCTGCTTCAG AGAAAACCCA GCACAGCTTG 
AACAACTCAC AGCTCAACTC TCTCCAACAC ACCCATCTAC TGGCCAACAG ACTAGAGCTG 
CCCTTCATGA TGATGCCCCA CCCTCTGCTT CCTGTTGGAC TTCCTCCTGC CTCTGTTGCC 
ATGGCAATGA ACCAGATGAA CCACCTTAAT ACAATTGCCA ACATGGTTGC CAGCGCACAG 
GTGCACAGCC CCGTCTCCAG GCCGACATCT GCCATCAAGC AGGAACGTTT TGAAGAAAGC 
CCCTCTCTGA CTCCATCTGT AGAGGGGATT GTTTCTCAAA AAACCGAACC TTCACCACAA 
CAAAGCAGTT CGGTTCCCAG TAGCCCCACA CACCCTTACA CACATTCTCC TCTAAAAACA 
GCATATGACG CTCATGATGA ACAAAGGGAG ACTGATTCAG CTCTGCATGT GAACAGACTT 
TCCAACGACA GGGTTGAGCA GAATGCAGTG AAGCCAGCAT TGTTTGAGAA GGTTCCAGCT 
CAGACGTTTC CCTCAGGCTT TCCTGCTTCT CTCCTGTTCA CTGACGGCCT TTCCTCTGTC 
GAGACGCTGC TCACTAATGT TCAGGGTCTG CTGAAGGTGG CTCTGGAAAA CGCACGTCTG 
CAAGAGAAGC AGCTTCAGCA GGAGAGGAGA GAGCTCAAGA TGGAGCTGTA CAGAGAGAGA 
GAGATGAGAG AGAGTCTGGA GAGACAGCTC ACCTCAGAGC TACGCACTCG AGCCACCATT 
CAGAGACGTC TGAAGAAGGA GAAGAAGGCA AAGAGGAGGT TACAGGAGGC GCTGGAGTAC 
GAGTCCAAGA GGAGAGGACA AATAGAGCAG GCTTTACAGC AGGCCACTTC ATCAGACTCT 
CTAACACACG ATCCAATCAG TCTGGAGATG GAGACAGAGC GATGCCGCAG CCCAGAGGAC 
AACTGCTTGT TACAAGAAAG CAGAACATAT ACGAAAAATC CAATCATATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333217.1
CDS	119..1252
Translation	MAEALKIQKMKMMMNLHKTHNGSEFDSDELNSNAGTVCSTLSWEREKHSSPASEKTQHSLNNSQLNSLQHTHLLANRLELPFMMMPHPLLPVGLPPASVAMAMNQMNHLNTIANMVASAQVHSPVSRPTSAIKQERFEESPSLTPSVEGIVSQKTEPSPQQSSSVPSSPTHPYTHSPLKTAYDAHDEQRETDSALHVNRLSNDRVEQNAVKPALFEKVPAQTFPSGFPASLLFTDGLSSVETLLTNVQGLLKVALENARLQEKQLQQERRELKMELYREREMRESLERQLTSELRTRATIQRRLKKEKKAKRRLQEALEYESKRRGQIEQALQQATSSDSLTHDPISLEMETERCRSPEDNCLLQESRTYTKNPIIY

Target ORF information:

RefSeq Version	XM_021477542.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dachshund b (dachb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477542.1

ORF Insert Sequence:

ATGGCTGAAG CTTTAAAGAT ACAGAAGATG AAGATGATGA TGAACCTGCA CAAGACTCAC 
AATGGCTCCG AATTCGATTC AGATGAGCTG AACTCTAACG CCGGTACAGT ATGCAGCACC 
CTGTCCTGGG AGAGAGAGAA ACATTCATCA CCTGCTTCAG AGAAAACCCA GCACAGCTTG 
AACAACTCAC AGCTCAACTC TCTCCAACAC ACCCATCTAC TGGCCAACAG ACTAGAGCTG 
CCCTTCATGA TGATGCCCCA CCCTCTGCTT CCTGTTGGAC TTCCTCCTGC CTCTGTTGCC 
ATGGCAATGA ACCAGATGAA CCACCTTAAT ACAATTGCCA ACATGGTTGC CAGCGCACAG 
GTGCACAGCC CCGTCTCCAG GCCGACATCT GCCATCAAGC AGGAACGTTT TGAAGAAAGC 
CCCTCTCTGA CTCCATCTGT AGAGGGGATT GTTTCTCAAA AAACCGAACC TTCACCACAA 
CAAAGCAGTT CGGTTCCCAG TAGCCCCACA CACCCTTACA CACATTCTCC TCTAAAAACA 
GCATATGACG CTCATGATGA ACAAAGGGAG ACTGATTCAG CTCTGCATGT GAACAGACTT 
TCCAACGACA GGGTTGAGCA GAATGCAGTG AAGCCAGCAT TGTTTGAGAA GGTTCCAGCT 
CAGACGTTTC CCTCAGGCTT TCCTGCTTCT CTCCTGTTCA CTGACGGCCT TTCCTCTGTC 
GAGACGCTGC TCACTAATGT TCAGGGTCTG CTGAAGGTGG CTCTGGAAAA CGCACGTCTG 
CAAGAGAAGC AGCTTCAGCA GGAGAGGAGA GAGCTCAAGA TGGAGCTGTA CAGAGAGAGA 
GAGATGAGAG AGAGTCTGGA GAGACAGCTC ACCTCAGAGC TACGCACTCG AGCCACCATT 
CAGAGACGTC TGAAGAAGGA GAAGAAGGCA AAGAGGAGGT TACAGGAGGC GCTGGAGTAC 
GAGTCCAAGA GGAGAGGACA AATAGAGCAG GCTTTACAGC AGGCCACTTC ATCAGACTCT 
CTAACACACG ATCCAATCAG TCTGGAGATG GAGACAGAGC GATGCCGCAG CCCAGAGGAC 
AACTGCTTGT TACAAGAAAG CAGAACATAT ACGAAAAATC CAATCATATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACHB ( NM_152956.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_694488.2 Danio rerio dachshund b (dachb), mRNA.

Price & Availability↑

CloneID	ODa53216
Clone ID Related Accession (Same CDS sequence)	NM_152956.2
Accession Version	NM_152956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1501862400000
Organism	Danio rerio(zebrafish)
Product	dachshund b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL935188.7. On Oct 29, 2015 this sequence version replaced NM_152956.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01008238.1, GDQH01002885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACCACTA TGGCCGTTCC CGCGACTCTT CCGGTTCACT CTGGGACTTC TGCGGTGTCC 
GGTAAACTCT TCAGGACCGA GCCGTTCTTC TCGAGTCCCG CTGAGGCTCC GCGTATCATC 
AACTCGCTGC CGAGCGGTGG GACCACAAAC GAGTGCAAGA TCGTGGAGGT TCACGGGGTT 
AAAGTGGCGT CTTTCAGTGT TGATGGGCAG GAGCTTATTT GTCTCCCTCA AGTATTTGAC 
CTTTTCCTGA AGCACCTGGT GGGCGGACTG CACACCGTGT ACACTAAACT CAAACGCTTG 
GATATAAACC CAGTGGTGTG CACCGTGGAG CAGGTGCGCG TGCTGCGCGG ACTCGGTGCC 
ATCCAGCCCG GAGTGAACCG CTGCAAACTC ATTTCCAGAA AAGATTTCGA AGCGCTGTAC 
AACGACTGCA CCAACGCAAG CTCTCGTCCA GGACGCCCAC CTAAGCGCTC CTATGGGGCC 
AGTGTTCAAG AAAGCCCCAG AATCCTCCAC CACAGAGCAA ACCTCCTGTC TCCAGCCCTG 
CTTTCACCCA CAGGTTTAAC AACAGCAGCT ATGGCTGAAG CTTTAAAGAT ACAGAAGATG 
AAGATGATGA TGAACCTGCA CAAGACTCAC AATGGCTCCG AATTCGATTC AGATGAGCTG 
AACTCTAACG CCGGTACAGT ATGCAGCACC CTGTCCTGGG AGAGAGAGAA ACATTCATCA 
CCTGCTTCAG AGAAAACCCA GCACAGCTTG AACAACTCAC AGCTCAACTC TCTCCAACAC 
ACCCATCTAC TGGCCAACAG ACTAGAGCTG CCCTTCATGA TGATGCCCCA CCCTCTGCTT 
CCTGTTGGAC TTCCTCCTGC CTCTGTTGCC ATGGCAATGA ACCAGATGAA CCACCTTAAT 
ACAATTGCCA ACATGGTTGC CAGCGCACAG GTGCACAGCC CCGTCTCCAG GCCGACATCT 
GCCATCAAGC AGGAACGTTT TGAAGAAAGC CCCTCTCTGA CTCCATCTGT AGAGGGGATT 
GTTTCTCAAA AAACCGAACC TTCACCACAA CAAAGCAGTT CGGTTCCCAG TAGCCCCACA 
CACCCTTACA CACATTCTCC TCTAAAAACA GCATATGACG CTCATGATGA ACAAAGGGAG 
ACTGATTCAG CTCTGCATGT GAACAGACTT TCCAACGACA GGGTTGAGCA GAATGCAGTG 
AAGCCAGCAT TGTTTGAGAA GGTTCCAGCT CAGACGTTTC CCTCAGGCTT TCCTGCTTCT 
CTCCTGTTCA CTGACGGCCT TTCCTCTGTC GAGACGCTGC TCACTAATGT TCAGGGTCTG 
CTGAAGGTGG CTCTGGAAAA CGCACGTCTG CAAGAGAAGC AGCTTCAGCA GGAGAGGAGA 
GAGCTCAAGA TGGAGCTGTA CAGAGAGAGA GAGATGAGAG AGAGTCTGGA GAGACAGCTC 
ACCTCAGAGC TACGCACTCG AGCCACCATT CAGAGACGTC TGAAGAAGGA GAAGAAGGCA 
AAGAGGAGGT TACAGGAGGC GCTGGAGTAC GAGTCCAAGA GGAGAGGACA AATAGAGCAG 
GCTTTACAGC AGGCCACTTC ATCAGACTCT CTAACACACG ATCCAATCAG TCTGGAGATG 
GAGACAGAGC GATGCCGCAG CCCAGAGGAC AACTGCTTGT TACAAGAAAG CAGAACATAT 
ACGAAAAATC CAATCATATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694488.2
CDS	136..1839
Translation	MTTMAVPATLPVHSGTSAVSGKLFRTEPFFSSPAEAPRIINSLPSGGTTNECKIVEVHGVKVASFSVDGQELICLPQVFDLFLKHLVGGLHTVYTKLKRLDINPVVCTVEQVRVLRGLGAIQPGVNRCKLISRKDFEALYNDCTNASSRPGRPPKRSYGASVQESPRILHHRANLLSPALLSPTGLTTAAMAEALKIQKMKMMMNLHKTHNGSEFDSDELNSNAGTVCSTLSWEREKHSSPASEKTQHSLNNSQLNSLQHTHLLANRLELPFMMMPHPLLPVGLPPASVAMAMNQMNHLNTIANMVASAQVHSPVSRPTSAIKQERFEESPSLTPSVEGIVSQKTEPSPQQSSSVPSSPTHPYTHSPLKTAYDAHDEQRETDSALHVNRLSNDRVEQNAVKPALFEKVPAQTFPSGFPASLLFTDGLSSVETLLTNVQGLLKVALENARLQEKQLQQERRELKMELYREREMRESLERQLTSELRTRATIQRRLKKEKKAKRRLQEALEYESKRRGQIEQALQQATSSDSLTHDPISLEMETERCRSPEDNCLLQESRTYTKNPIIY

Target ORF information:

RefSeq Version	NM_152956.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dachshund b (dachb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152956.2

ORF Insert Sequence:

ATGACCACTA TGGCCGTTCC CGCGACTCTT CCGGTTCACT CTGGGACTTC TGCGGTGTCC 
GGTAAACTCT TCAGGACCGA GCCGTTCTTC TCGAGTCCCG CTGAGGCTCC GCGTATCATC 
AACTCGCTGC CGAGCGGTGG GACCACAAAC GAGTGCAAGA TCGTGGAGGT TCACGGGGTT 
AAAGTGGCGT CTTTCAGTGT TGATGGGCAG GAGCTTATTT GTCTCCCTCA AGTATTTGAC 
CTTTTCCTGA AGCACCTGGT GGGCGGACTG CACACCGTGT ACACTAAACT CAAACGCTTG 
GATATAAACC CAGTGGTGTG CACCGTGGAG CAGGTGCGCG TGCTGCGCGG ACTCGGTGCC 
ATCCAGCCCG GAGTGAACCG CTGCAAACTC ATTTCCAGAA AAGATTTCGA AGCGCTGTAC 
AACGACTGCA CCAACGCAAG CTCTCGTCCA GGACGCCCAC CTAAGCGCTC CTATGGGGCC 
AGTGTTCAAG AAAGCCCCAG AATCCTCCAC CACAGAGCAA ACCTCCTGTC TCCAGCCCTG 
CTTTCACCCA CAGGTTTAAC AACAGCAGCT ATGGCTGAAG CTTTAAAGAT ACAGAAGATG 
AAGATGATGA TGAACCTGCA CAAGACTCAC AATGGCTCCG AATTCGATTC AGATGAGCTG 
AACTCTAACG CCGGTACAGT ATGCAGCACC CTGTCCTGGG AGAGAGAGAA ACATTCATCA 
CCTGCTTCAG AGAAAACCCA GCACAGCTTG AACAACTCAC AGCTCAACTC TCTCCAACAC 
ACCCATCTAC TGGCCAACAG ACTAGAGCTG CCCTTCATGA TGATGCCCCA CCCTCTGCTT 
CCTGTTGGAC TTCCTCCTGC CTCTGTTGCC ATGGCAATGA ACCAGATGAA CCACCTTAAT 
ACAATTGCCA ACATGGTTGC CAGCGCACAG GTGCACAGCC CCGTCTCCAG GCCGACATCT 
GCCATCAAGC AGGAACGTTT TGAAGAAAGC CCCTCTCTGA CTCCATCTGT AGAGGGGATT 
GTTTCTCAAA AAACCGAACC TTCACCACAA CAAAGCAGTT CGGTTCCCAG TAGCCCCACA 
CACCCTTACA CACATTCTCC TCTAAAAACA GCATATGACG CTCATGATGA ACAAAGGGAG 
ACTGATTCAG CTCTGCATGT GAACAGACTT TCCAACGACA GGGTTGAGCA GAATGCAGTG 
AAGCCAGCAT TGTTTGAGAA GGTTCCAGCT CAGACGTTTC CCTCAGGCTT TCCTGCTTCT 
CTCCTGTTCA CTGACGGCCT TTCCTCTGTC GAGACGCTGC TCACTAATGT TCAGGGTCTG 
CTGAAGGTGG CTCTGGAAAA CGCACGTCTG CAAGAGAAGC AGCTTCAGCA GGAGAGGAGA 
GAGCTCAAGA TGGAGCTGTA CAGAGAGAGA GAGATGAGAG AGAGTCTGGA GAGACAGCTC 
ACCTCAGAGC TACGCACTCG AGCCACCATT CAGAGACGTC TGAAGAAGGA GAAGAAGGCA 
AAGAGGAGGT TACAGGAGGC GCTGGAGTAC GAGTCCAAGA GGAGAGGACA AATAGAGCAG 
GCTTTACAGC AGGCCACTTC ATCAGACTCT CTAACACACG ATCCAATCAG TCTGGAGATG 
GAGACAGAGC GATGCCGCAG CCCAGAGGAC AACTGCTTGT TACAAGAAAG CAGAACATAT 
ACGAAAAATC CAATCATATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACHB ( XM_009305776.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304051.1 Danio rerio dachshund b (dachb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39553
Clone ID Related Accession (Same CDS sequence)	XM_009305776.2 , XM_009305776.3
Accession Version	XM_009305776.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	dachshund b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTA TGGCCGTTCC CGCGACTCTT CCGGTTCACT CTGGGACTTC TGCGGTGTCC 
GGTAAACTCT TCAGGACCGA GCCGTTCTTC TCGAGTCCCG CTGAGGCTCC GCGTATCATC 
AACTCGCTGC CGAGCGGTGG GACCACAAAC GAGTGCAAGA TCGTGGAGGT TCACGGGGTT 
AAAGTGGCGT CTTTCAGTGT TGATGGGCAG GAGCTTATTT GTCTCCCTCA AGTATTTGAC 
CTTTTCCTGA AGCACCTGGT GGGCGGACTG CACACCGTGT ACACTAAACT CAAACGCTTG 
GATATAAACC CAGTGGTGTG CACCGTGGAG CAGGTGCGCG TGCTGCGCGG ACTCGGTGCC 
ATCCAGCCCG GAGTGAACCG CTGCAAACTC ATTTCCAGAA AAGATTTCGA AGCGCTGTAC 
AACGACTGCA CCAACGCAAG CTCTCGTCCA GGACGCCCAC CTAAGCGCTC CTATGGGGCC 
AGTGTTCAAG AAAGCCCCAG AATCCTCCAC CACAGAGCAA ACCTCCTGTC TCCAGCCCTG 
CTTTCACCCA CAGGTTTAAC AACAGCAGCT ATGGCTGAAG CTTTAAAGAT ACAGAAGATG 
AAGATGATGA TGAACCTGCA CAAGACTCAC AATGGCTCCG AATTCGATTC AGATGAGCTG 
AACTCTAACG CCGGTACAGT ATGCAGCACC CTGTCCTGGG AGAGAGAGAA ACATTCATCA 
CCTGCTTCAG AGAAAACCCA GCACAGCTTG AACAACTCAC AGCTCAACTC TCTCCAACAC 
ACCCATCTAC TGGCCAACAG ACTAGAGCTG CCCTTCATGA TGATGCCCCA CCCTCTGCTT 
CCTGTTGGAC TTCCTCCTGC CTCTGTTGCC ATGGCAATGA ACCAGATGAA CCACCTTAAT 
ACAATTGCCA ACATGGTTGC CAGCGCACAG GTGCACAGCC CCGTCTCCAG GCCGACATCT 
GCCATCAAGG AACGTTTTGA AGAAAGCCCC TCTCTGACTC CATCTGTAGA GGGGATTGTT 
TCTCAAAAAA CCGAACCTTC ACCACAACAA AGCAGTTCGG TTCCCAGTAG CCCCACACAC 
CCTTACACAC ATTCTCCTCT AAAAACAGCA TATGACGCTC ATGATGAACA AAGGGAGACT 
GATTCAGCTC TGCATGTGAA CAGACTTTCC AACGACAGGG TTGAGCAGAA TGCAGTGAAG 
CCAGCATTGT TTGAGAAGGT TCCAGCTCAG ACGTTTCCCT CAGGCTTTCC TGCTTCTCTC 
CTGTTCACTG ACGGCCTTTC CTCTGTCGAG ACGCTGCTCA CTAATGTTCA GGGTCTGCTG 
AAGGTGGCTC TGGAAAACGC ACGTCTGCAA GAGAAGCAGC TTCAGCAGGA GAGGAGAGAG 
CTCAAGATGG AGCTGTACAG AGAGAGAGAG ATGAGAGAGA GTCTGGAGAG ACAGCTCACC 
TCAGAGCTAC GCACTCGAGC CACCATTCAG AGACGTCTGA AGAAGGAGAA GAAGGCAAAG 
AGGAGGTTAC AGGAGGCGCT GGAGTACGAG TCCAAGAGGA GAGGACAAAT AGAGCAGGCT 
TTACAGCAGG CCACTTCATC AGACTCTCTA ACACACGATC CAATCAGTCT GGAGATGGAG 
ACAGAGCGAT GCCGCAGCCC AGAGGACAAC TGCTTGTTAC AAGAAAGCAG AACATATACG 
AAAAATCCAA TCATATACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304051.1
CDS	177..1877
Translation	MTTMAVPATLPVHSGTSAVSGKLFRTEPFFSSPAEAPRIINSLPSGGTTNECKIVEVHGVKVASFSVDGQELICLPQVFDLFLKHLVGGLHTVYTKLKRLDINPVVCTVEQVRVLRGLGAIQPGVNRCKLISRKDFEALYNDCTNASSRPGRPPKRSYGASVQESPRILHHRANLLSPALLSPTGLTTAAMAEALKIQKMKMMMNLHKTHNGSEFDSDELNSNAGTVCSTLSWEREKHSSPASEKTQHSLNNSQLNSLQHTHLLANRLELPFMMMPHPLLPVGLPPASVAMAMNQMNHLNTIANMVASAQVHSPVSRPTSAIKERFEESPSLTPSVEGIVSQKTEPSPQQSSSVPSSPTHPYTHSPLKTAYDAHDEQRETDSALHVNRLSNDRVEQNAVKPALFEKVPAQTFPSGFPASLLFTDGLSSVETLLTNVQGLLKVALENARLQEKQLQQERRELKMELYREREMRESLERQLTSELRTRATIQRRLKKEKKAKRRLQEALEYESKRRGQIEQALQQATSSDSLTHDPISLEMETERCRSPEDNCLLQESRTYTKNPIIY

Target ORF information:

RefSeq Version	XM_009305776.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dachshund b (dachb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305776.2

ORF Insert Sequence:

ATGACCACTA TGGCCGTTCC CGCGACTCTT CCGGTTCACT CTGGGACTTC TGCGGTGTCC 
GGTAAACTCT TCAGGACCGA GCCGTTCTTC TCGAGTCCCG CTGAGGCTCC GCGTATCATC 
AACTCGCTGC CGAGCGGTGG GACCACAAAC GAGTGCAAGA TCGTGGAGGT TCACGGGGTT 
AAAGTGGCGT CTTTCAGTGT TGATGGGCAG GAGCTTATTT GTCTCCCTCA AGTATTTGAC 
CTTTTCCTGA AGCACCTGGT GGGCGGACTG CACACCGTGT ACACTAAACT CAAACGCTTG 
GATATAAACC CAGTGGTGTG CACCGTGGAG CAGGTGCGCG TGCTGCGCGG ACTCGGTGCC 
ATCCAGCCCG GAGTGAACCG CTGCAAACTC ATTTCCAGAA AAGATTTCGA AGCGCTGTAC 
AACGACTGCA CCAACGCAAG CTCTCGTCCA GGACGCCCAC CTAAGCGCTC CTATGGGGCC 
AGTGTTCAAG AAAGCCCCAG AATCCTCCAC CACAGAGCAA ACCTCCTGTC TCCAGCCCTG 
CTTTCACCCA CAGGTTTAAC AACAGCAGCT ATGGCTGAAG CTTTAAAGAT ACAGAAGATG 
AAGATGATGA TGAACCTGCA CAAGACTCAC AATGGCTCCG AATTCGATTC AGATGAGCTG 
AACTCTAACG CCGGTACAGT ATGCAGCACC CTGTCCTGGG AGAGAGAGAA ACATTCATCA 
CCTGCTTCAG AGAAAACCCA GCACAGCTTG AACAACTCAC AGCTCAACTC TCTCCAACAC 
ACCCATCTAC TGGCCAACAG ACTAGAGCTG CCCTTCATGA TGATGCCCCA CCCTCTGCTT 
CCTGTTGGAC TTCCTCCTGC CTCTGTTGCC ATGGCAATGA ACCAGATGAA CCACCTTAAT 
ACAATTGCCA ACATGGTTGC CAGCGCACAG GTGCACAGCC CCGTCTCCAG GCCGACATCT 
GCCATCAAGG AACGTTTTGA AGAAAGCCCC TCTCTGACTC CATCTGTAGA GGGGATTGTT 
TCTCAAAAAA CCGAACCTTC ACCACAACAA AGCAGTTCGG TTCCCAGTAG CCCCACACAC 
CCTTACACAC ATTCTCCTCT AAAAACAGCA TATGACGCTC ATGATGAACA AAGGGAGACT 
GATTCAGCTC TGCATGTGAA CAGACTTTCC AACGACAGGG TTGAGCAGAA TGCAGTGAAG 
CCAGCATTGT TTGAGAAGGT TCCAGCTCAG ACGTTTCCCT CAGGCTTTCC TGCTTCTCTC 
CTGTTCACTG ACGGCCTTTC CTCTGTCGAG ACGCTGCTCA CTAATGTTCA GGGTCTGCTG 
AAGGTGGCTC TGGAAAACGC ACGTCTGCAA GAGAAGCAGC TTCAGCAGGA GAGGAGAGAG 
CTCAAGATGG AGCTGTACAG AGAGAGAGAG ATGAGAGAGA GTCTGGAGAG ACAGCTCACC 
TCAGAGCTAC GCACTCGAGC CACCATTCAG AGACGTCTGA AGAAGGAGAA GAAGGCAAAG 
AGGAGGTTAC AGGAGGCGCT GGAGTACGAG TCCAAGAGGA GAGGACAAAT AGAGCAGGCT 
TTACAGCAGG CCACTTCATC AGACTCTCTA ACACACGATC CAATCAGTCT GGAGATGGAG 
ACAGAGCGAT GCCGCAGCCC AGAGGACAAC TGCTTGTTAC AAGAAAGCAG AACATATACG 
AAAAATCCAA TCATATACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACHB ( XM_009305776.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304051.1 Danio rerio dachshund b (dachb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39553
Clone ID Related Accession (Same CDS sequence)	XM_009305776.2 , XM_009305776.3
Accession Version	XM_009305776.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	dachshund b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305776.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTA TGGCCGTTCC CGCGACTCTT CCGGTTCACT CTGGGACTTC TGCGGTGTCC 
GGTAAACTCT TCAGGACCGA GCCGTTCTTC TCGAGTCCCG CTGAGGCTCC GCGTATCATC 
AACTCGCTGC CGAGCGGTGG GACCACAAAC GAGTGCAAGA TCGTGGAGGT TCACGGGGTT 
AAAGTGGCGT CTTTCAGTGT TGATGGGCAG GAGCTTATTT GTCTCCCTCA AGTATTTGAC 
CTTTTCCTGA AGCACCTGGT GGGCGGACTG CACACCGTGT ACACTAAACT CAAACGCTTG 
GATATAAACC CAGTGGTGTG CACCGTGGAG CAGGTGCGCG TGCTGCGCGG ACTCGGTGCC 
ATCCAGCCCG GAGTGAACCG CTGCAAACTC ATTTCCAGAA AAGATTTCGA AGCGCTGTAC 
AACGACTGCA CCAACGCAAG CTCTCGTCCA GGACGCCCAC CTAAGCGCTC CTATGGGGCC 
AGTGTTCAAG AAAGCCCCAG AATCCTCCAC CACAGAGCAA ACCTCCTGTC TCCAGCCCTG 
CTTTCACCCA CAGGTTTAAC AACAGCAGCT ATGGCTGAAG CTTTAAAGAT ACAGAAGATG 
AAGATGATGA TGAACCTGCA CAAGACTCAC AATGGCTCCG AATTCGATTC AGATGAGCTG 
AACTCTAACG CCGGTACAGT ATGCAGCACC CTGTCCTGGG AGAGAGAGAA ACATTCATCA 
CCTGCTTCAG AGAAAACCCA GCACAGCTTG AACAACTCAC AGCTCAACTC TCTCCAACAC 
ACCCATCTAC TGGCCAACAG ACTAGAGCTG CCCTTCATGA TGATGCCCCA CCCTCTGCTT 
CCTGTTGGAC TTCCTCCTGC CTCTGTTGCC ATGGCAATGA ACCAGATGAA CCACCTTAAT 
ACAATTGCCA ACATGGTTGC CAGCGCACAG GTGCACAGCC CCGTCTCCAG GCCGACATCT 
GCCATCAAGG AACGTTTTGA AGAAAGCCCC TCTCTGACTC CATCTGTAGA GGGGATTGTT 
TCTCAAAAAA CCGAACCTTC ACCACAACAA AGCAGTTCGG TTCCCAGTAG CCCCACACAC 
CCTTACACAC ATTCTCCTCT AAAAACAGCA TATGACGCTC ATGATGAACA AAGGGAGACT 
GATTCAGCTC TGCATGTGAA CAGACTTTCC AACGACAGGG TTGAGCAGAA TGCAGTGAAG 
CCAGCATTGT TTGAGAAGGT TCCAGCTCAG ACGTTTCCCT CAGGCTTTCC TGCTTCTCTC 
CTGTTCACTG ACGGCCTTTC CTCTGTCGAG ACGCTGCTCA CTAATGTTCA GGGTCTGCTG 
AAGGTGGCTC TGGAAAACGC ACGTCTGCAA GAGAAGCAGC TTCAGCAGGA GAGGAGAGAG 
CTCAAGATGG AGCTGTACAG AGAGAGAGAG ATGAGAGAGA GTCTGGAGAG ACAGCTCACC 
TCAGAGCTAC GCACTCGAGC CACCATTCAG AGACGTCTGA AGAAGGAGAA GAAGGCAAAG 
AGGAGGTTAC AGGAGGCGCT GGAGTACGAG TCCAAGAGGA GAGGACAAAT AGAGCAGGCT 
TTACAGCAGG CCACTTCATC AGACTCTCTA ACACACGATC CAATCAGTCT GGAGATGGAG 
ACAGAGCGAT GCCGCAGCCC AGAGGACAAC TGCTTGTTAC AAGAAAGCAG AACATATACG 
AAAAATCCAA TCATATACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304051.1
CDS	167..1867
Translation	MTTMAVPATLPVHSGTSAVSGKLFRTEPFFSSPAEAPRIINSLPSGGTTNECKIVEVHGVKVASFSVDGQELICLPQVFDLFLKHLVGGLHTVYTKLKRLDINPVVCTVEQVRVLRGLGAIQPGVNRCKLISRKDFEALYNDCTNASSRPGRPPKRSYGASVQESPRILHHRANLLSPALLSPTGLTTAAMAEALKIQKMKMMMNLHKTHNGSEFDSDELNSNAGTVCSTLSWEREKHSSPASEKTQHSLNNSQLNSLQHTHLLANRLELPFMMMPHPLLPVGLPPASVAMAMNQMNHLNTIANMVASAQVHSPVSRPTSAIKERFEESPSLTPSVEGIVSQKTEPSPQQSSSVPSSPTHPYTHSPLKTAYDAHDEQRETDSALHVNRLSNDRVEQNAVKPALFEKVPAQTFPSGFPASLLFTDGLSSVETLLTNVQGLLKVALENARLQEKQLQQERRELKMELYREREMRESLERQLTSELRTRATIQRRLKKEKKAKRRLQEALEYESKRRGQIEQALQQATSSDSLTHDPISLEMETERCRSPEDNCLLQESRTYTKNPIIY

Target ORF information:

RefSeq Version	XM_009305776.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dachshund b (dachb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305776.3

ORF Insert Sequence:

ATGACCACTA TGGCCGTTCC CGCGACTCTT CCGGTTCACT CTGGGACTTC TGCGGTGTCC 
GGTAAACTCT TCAGGACCGA GCCGTTCTTC TCGAGTCCCG CTGAGGCTCC GCGTATCATC 
AACTCGCTGC CGAGCGGTGG GACCACAAAC GAGTGCAAGA TCGTGGAGGT TCACGGGGTT 
AAAGTGGCGT CTTTCAGTGT TGATGGGCAG GAGCTTATTT GTCTCCCTCA AGTATTTGAC 
CTTTTCCTGA AGCACCTGGT GGGCGGACTG CACACCGTGT ACACTAAACT CAAACGCTTG 
GATATAAACC CAGTGGTGTG CACCGTGGAG CAGGTGCGCG TGCTGCGCGG ACTCGGTGCC 
ATCCAGCCCG GAGTGAACCG CTGCAAACTC ATTTCCAGAA AAGATTTCGA AGCGCTGTAC 
AACGACTGCA CCAACGCAAG CTCTCGTCCA GGACGCCCAC CTAAGCGCTC CTATGGGGCC 
AGTGTTCAAG AAAGCCCCAG AATCCTCCAC CACAGAGCAA ACCTCCTGTC TCCAGCCCTG 
CTTTCACCCA CAGGTTTAAC AACAGCAGCT ATGGCTGAAG CTTTAAAGAT ACAGAAGATG 
AAGATGATGA TGAACCTGCA CAAGACTCAC AATGGCTCCG AATTCGATTC AGATGAGCTG 
AACTCTAACG CCGGTACAGT ATGCAGCACC CTGTCCTGGG AGAGAGAGAA ACATTCATCA 
CCTGCTTCAG AGAAAACCCA GCACAGCTTG AACAACTCAC AGCTCAACTC TCTCCAACAC 
ACCCATCTAC TGGCCAACAG ACTAGAGCTG CCCTTCATGA TGATGCCCCA CCCTCTGCTT 
CCTGTTGGAC TTCCTCCTGC CTCTGTTGCC ATGGCAATGA ACCAGATGAA CCACCTTAAT 
ACAATTGCCA ACATGGTTGC CAGCGCACAG GTGCACAGCC CCGTCTCCAG GCCGACATCT 
GCCATCAAGG AACGTTTTGA AGAAAGCCCC TCTCTGACTC CATCTGTAGA GGGGATTGTT 
TCTCAAAAAA CCGAACCTTC ACCACAACAA AGCAGTTCGG TTCCCAGTAG CCCCACACAC 
CCTTACACAC ATTCTCCTCT AAAAACAGCA TATGACGCTC ATGATGAACA AAGGGAGACT 
GATTCAGCTC TGCATGTGAA CAGACTTTCC AACGACAGGG TTGAGCAGAA TGCAGTGAAG 
CCAGCATTGT TTGAGAAGGT TCCAGCTCAG ACGTTTCCCT CAGGCTTTCC TGCTTCTCTC 
CTGTTCACTG ACGGCCTTTC CTCTGTCGAG ACGCTGCTCA CTAATGTTCA GGGTCTGCTG 
AAGGTGGCTC TGGAAAACGC ACGTCTGCAA GAGAAGCAGC TTCAGCAGGA GAGGAGAGAG 
CTCAAGATGG AGCTGTACAG AGAGAGAGAG ATGAGAGAGA GTCTGGAGAG ACAGCTCACC 
TCAGAGCTAC GCACTCGAGC CACCATTCAG AGACGTCTGA AGAAGGAGAA GAAGGCAAAG 
AGGAGGTTAC AGGAGGCGCT GGAGTACGAG TCCAAGAGGA GAGGACAAAT AGAGCAGGCT 
TTACAGCAGG CCACTTCATC AGACTCTCTA ACACACGATC CAATCAGTCT GGAGATGGAG 
ACAGAGCGAT GCCGCAGCCC AGAGGACAAC TGCTTGTTAC AAGAAAGCAG AACATATACG 
AAAAATCCAA TCATATACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACHB ( XM_017356588.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212077.1 Danio rerio dachshund b (dachb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa53669
Clone ID Related Accession (Same CDS sequence)	XM_017356588.1
Accession Version	XM_017356588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	dachshund b isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTA TGGCCGTTCC CGCGACTCTT CCGGTTCACT CTGGGACTTC TGCGGTGTCC 
GGTAAACTCT TCAGGACCGA GCCGTTCTTC TCGAGTCCCG CTGAGGCTCC GCGTATCATC 
AACTCGCTGC CGAGCGGTGG GACCACAAAC GAGTGCAAGA TCGTGGAGGT TCACGGGGTT 
AAAGTGGCGT CTTTCAGTGT TGATGGGCAG GAGCTTATTT GTCTCCCTCA AGTATTTGAC 
CTTTTCCTGA AGCACCTGGT GGGCGGACTG CACACCGTGT ACACTAAACT CAAACGCTTG 
GATATAAACC CAGTGGTGTG CACCGTGGAG CAGGTGCGCG TGCTGCGCGG ACTCGGTGCC 
ATCCAGCCCG GAGTGAACCG CTGCAAACTC ATTTCCAGAA AAGATTTCGA AGCGCTGTAC 
AACGACTGCA CCAACGCAAG CTCTCGTCCA GGACGCCCAC CTAAGCGCTC CTATGGGGCC 
AGTGTTCAAG AAAGCCCCAG AATCCTCCAC CACAGAGCAA ACCTCCTGTC TCCAGCCCTG 
CTTTCACCCA CAGGTTTAAC AACAGCAGCT ATGGCTGAAG CTTTAAAGAT ACAGAAGATG 
AAGATGATGA TGAACCTGCA CAAGACTCAC AATGGCTCCG AATTCGATTC AGATGAGCTG 
AACTCTAACG CCGTATGCAG CACCCTGTCC TGGGAGAGAG AGAAACATTC ATCACCTGCT 
TCAGAGAAAA CCCAGCACAG CTTGAACAAC TCACAGCTCA ACTCTCTCCA ACACACCCAT 
CTACTGGCCA ACAGACTAGA GCTGCCCTTC ATGATGATGC CCCACCCTCT GCTTCCTGTT 
GGACTTCCTC CTGCCTCTGT TGCCATGGCA ATGAACCAGA TGAACCACCT TAATACAATT 
GCCAACATGG TTGCCAGCGC ACAGGTGCAC AGCCCCGTCT CCAGGCCGAC ATCTGCCATC 
AAGCAGGAAC GTTTTGAAGA AAGCCCCTCT CTGACTCCAT CTGTAGAGGG GATTGTTTCT 
CAAAAAACCG AACCTTCACC ACAACAAAGC AGTTCGGTTC CCAGTAGCCC CACACACCCT 
TACACACATT CTCCTCTAAA AACAGCATAT GACGCTCATG ATGAACAAAG GGAGACTGAT 
TCAGCTCTGC ATGTGAACAG ACTTTCCAAC GACAGGGTTG AGCAGAATGC AGTGAAGCCA 
GCATTGTTTG AGAAGGTTCC AGCTCAGACG TTTCCCTCAG GCTTTCCTGC TTCTCTCCTG 
TTCACTGACG GCCTTTCCTC TGTCGAGACG CTGCTCACTA ATGTTCAGGG TCTGCTGAAG 
GTGGCTCTGG AAAACGCACG TCTGCAAGAG AAGCAGCTTC AGCAGGAGAG GAGAGAGCTC 
AAGATGGAGC TGTACAGAGA GAGAGAGATG AGAGAGAGTC TGGAGAGACA GCTCACCTCA 
GAGCTACGCA CTCGAGCCAC CATTCAGAGA CGTCTGAAGA AGGAGAAGAA GGCAAAGAGG 
AGGTTACAGG AGGCGCTGGA GTACGAGTCC AAGAGGAGAG GACAAATAGA GCAGGCTTTA 
CAGCAGGCCA CTTCATCAGA CTCTCTAACA CACGATCCAA TCAGTCTGGA GATGGAGACA 
GAGCGATGCC GCAGCCCAGA GGACAACTGC TTGTTACAAG AAAGCAGAAC ATATACGAAA 
AATCCAATCA TATACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212077.1
CDS	177..1874
Translation	MTTMAVPATLPVHSGTSAVSGKLFRTEPFFSSPAEAPRIINSLPSGGTTNECKIVEVHGVKVASFSVDGQELICLPQVFDLFLKHLVGGLHTVYTKLKRLDINPVVCTVEQVRVLRGLGAIQPGVNRCKLISRKDFEALYNDCTNASSRPGRPPKRSYGASVQESPRILHHRANLLSPALLSPTGLTTAAMAEALKIQKMKMMMNLHKTHNGSEFDSDELNSNAVCSTLSWEREKHSSPASEKTQHSLNNSQLNSLQHTHLLANRLELPFMMMPHPLLPVGLPPASVAMAMNQMNHLNTIANMVASAQVHSPVSRPTSAIKQERFEESPSLTPSVEGIVSQKTEPSPQQSSSVPSSPTHPYTHSPLKTAYDAHDEQRETDSALHVNRLSNDRVEQNAVKPALFEKVPAQTFPSGFPASLLFTDGLSSVETLLTNVQGLLKVALENARLQEKQLQQERRELKMELYREREMRESLERQLTSELRTRATIQRRLKKEKKAKRRLQEALEYESKRRGQIEQALQQATSSDSLTHDPISLEMETERCRSPEDNCLLQESRTYTKNPIIY

Target ORF information:

RefSeq Version	XM_017356588.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dachshund b (dachb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356588.1

ORF Insert Sequence:

ATGACCACTA TGGCCGTTCC CGCGACTCTT CCGGTTCACT CTGGGACTTC TGCGGTGTCC 
GGTAAACTCT TCAGGACCGA GCCGTTCTTC TCGAGTCCCG CTGAGGCTCC GCGTATCATC 
AACTCGCTGC CGAGCGGTGG GACCACAAAC GAGTGCAAGA TCGTGGAGGT TCACGGGGTT 
AAAGTGGCGT CTTTCAGTGT TGATGGGCAG GAGCTTATTT GTCTCCCTCA AGTATTTGAC 
CTTTTCCTGA AGCACCTGGT GGGCGGACTG CACACCGTGT ACACTAAACT CAAACGCTTG 
GATATAAACC CAGTGGTGTG CACCGTGGAG CAGGTGCGCG TGCTGCGCGG ACTCGGTGCC 
ATCCAGCCCG GAGTGAACCG CTGCAAACTC ATTTCCAGAA AAGATTTCGA AGCGCTGTAC 
AACGACTGCA CCAACGCAAG CTCTCGTCCA GGACGCCCAC CTAAGCGCTC CTATGGGGCC 
AGTGTTCAAG AAAGCCCCAG AATCCTCCAC CACAGAGCAA ACCTCCTGTC TCCAGCCCTG 
CTTTCACCCA CAGGTTTAAC AACAGCAGCT ATGGCTGAAG CTTTAAAGAT ACAGAAGATG 
AAGATGATGA TGAACCTGCA CAAGACTCAC AATGGCTCCG AATTCGATTC AGATGAGCTG 
AACTCTAACG CCGTATGCAG CACCCTGTCC TGGGAGAGAG AGAAACATTC ATCACCTGCT 
TCAGAGAAAA CCCAGCACAG CTTGAACAAC TCACAGCTCA ACTCTCTCCA ACACACCCAT 
CTACTGGCCA ACAGACTAGA GCTGCCCTTC ATGATGATGC CCCACCCTCT GCTTCCTGTT 
GGACTTCCTC CTGCCTCTGT TGCCATGGCA ATGAACCAGA TGAACCACCT TAATACAATT 
GCCAACATGG TTGCCAGCGC ACAGGTGCAC AGCCCCGTCT CCAGGCCGAC ATCTGCCATC 
AAGCAGGAAC GTTTTGAAGA AAGCCCCTCT CTGACTCCAT CTGTAGAGGG GATTGTTTCT 
CAAAAAACCG AACCTTCACC ACAACAAAGC AGTTCGGTTC CCAGTAGCCC CACACACCCT 
TACACACATT CTCCTCTAAA AACAGCATAT GACGCTCATG ATGAACAAAG GGAGACTGAT 
TCAGCTCTGC ATGTGAACAG ACTTTCCAAC GACAGGGTTG AGCAGAATGC AGTGAAGCCA 
GCATTGTTTG AGAAGGTTCC AGCTCAGACG TTTCCCTCAG GCTTTCCTGC TTCTCTCCTG 
TTCACTGACG GCCTTTCCTC TGTCGAGACG CTGCTCACTA ATGTTCAGGG TCTGCTGAAG 
GTGGCTCTGG AAAACGCACG TCTGCAAGAG AAGCAGCTTC AGCAGGAGAG GAGAGAGCTC 
AAGATGGAGC TGTACAGAGA GAGAGAGATG AGAGAGAGTC TGGAGAGACA GCTCACCTCA 
GAGCTACGCA CTCGAGCCAC CATTCAGAGA CGTCTGAAGA AGGAGAAGAA GGCAAAGAGG 
AGGTTACAGG AGGCGCTGGA GTACGAGTCC AAGAGGAGAG GACAAATAGA GCAGGCTTTA 
CAGCAGGCCA CTTCATCAGA CTCTCTAACA CACGATCCAA TCAGTCTGGA GATGGAGACA 
GAGCGATGCC GCAGCCCAGA GGACAACTGC TTGTTACAAG AAAGCAGAAC ATATACGAAA 
AATCCAATCA TATACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Exome sequencing and functional analyses suggest that SIX6 is a gene involved in an altered proliferation-differentiation balance early in life and optic nerve degeneration at old age.
Human molecular genetics23(5)1320-32(2014 Mar)
Iglesias AI,Springelkamp H,van der Linde H,Severijnen LA,Amin N,Oostra B,Kockx CE,van den Hout MC,van Ijcken WF,Hofman A,Uitterlinden AG,Verdijk RM,Klaver CC,Willemsen R,van Duijn CM

Dachshund homologues play a conserved role in islet cell development.
Developmental biology348(2)143-52(2010 Dec)
Kalousova A,Mavropoulos A,Adams BA,Nekrep N,Li Z,Krauss S,Stainier DY,German MS

The transcription factor six1 inhibits neuronal and promotes hair cell fate in the developing zebrafish (Danio rerio) inner ear.
The Journal of neuroscience : the official journal of the Society for Neuroscience26(41)10438-51(2006 Oct)
Bricaud O,Collazo A

Isolation of three zebrafish dachshund homologues and their expression in sensory organs, the central nervous system and pectoral fin buds.
Mechanisms of development112(1-2)183-9(2002 Mar)
Hammond KL,Hill RE,Whitfield TT,Currie PD

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DACHB cDNA ORF clone, Danio rerio(Zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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