abcb4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	abcb4
Entrez Gene ID	100136865
Full Name	ATP-binding cassette, sub-family B (MDR/TAP), member 4
Synonyms	zgc:172149
General protein information	Preferred Names ATP-binding cassette, sub-family B (MDR/TAP), member 4 Names ATP-binding cassette, sub-family B (MDR/TAP), member 4 ABC efflux transporter 4 abcb1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114583.2	NP_001108055.2	ATP-binding cassette, sub-family B (MDR/TAP), member 4 isoform 2
NM_001316714.1	NP_001303643.1	ATP-binding cassette, sub-family B (MDR/TAP), member 4 isoform 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	abcb4	NP_001108055.1
Oryza sativa (rice)	Os04g0642000	NP_001054045.1

Gene Ontology
Bibliography

Related articles in PubMed

Modulation of the multixenobiotic resistance mechanism in Danio rerio hepatocyte culture (ZF-L) after exposure to glyphosate and Roundup
Fernanda Moreira L, Juliana Zomer S, Marta Marques S
Chemosphere228159-165(2019 Aug)

Altered Gene expression of ABC transporters, nuclear receptors and oxidative stress signaling in zebrafish embryos exposed to CdTe quantum dots.
Tian J, Hu J, Liu G, Yin H, Chen M, Miao P, Bai P, Yin J
Environmental pollution (Barking, Essex : 1987)244588-599(2019 Jan)

Accumulation, metabolite and active defence system responses of fluoxetine in zebrafish embryos: Influence of multiwalled carbon nanotubes with different functional groups.
Yan Z, Sun H, Jiang R, Dong H, Yang H, Liu J, Lu G, Ji Y
Aquatic toxicology (Amsterdam, Netherlands)205204-212(2018 Dec)

From mRNA Expression of Drug Disposition Genes to In Vivo Assessment of CYP-Mediated Biotransformation during Zebrafish Embryonic and Larval Development.
Verbueken E, Bars C, Ball JS, Periz-Stanacev J, Marei WFA, Tochwin A, Gabriëls IJ, Michiels EDG, Stinckens E, Vergauwen L, Knapen D, Van Ginneken CJ, Van Cruchten SJ
International journal of molecular sciences19(12)(2018 Dec)

Effects of starch-coating of magnetite nanoparticles on cellular uptake, toxicity and gene expression profiles in adult zebrafish.
Zheng M, Lu J, Zhao D
The Science of the total environment622-623930-941(2018 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following abcb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the abcb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa60481	NM_001114583.2 Latest version!	Danio rerio ATP-binding cassette, sub-family B (MDR/TAP), member 4 (abcb4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa21533	NM_001316714.1 Latest version!	Danio rerio ATP-binding cassette, sub-family B (MDR/TAP), member 4 (abcb4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

abcb4 ( NM_001114583.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001108055.2 Danio rerio ATP-binding cassette, sub-family B (MDR/TAP), member 4 (abcb4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa60481
Clone ID Related Accession (Same CDS sequence)	NM_001114583.2
Accession Version	NM_001114583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576771200000
Organism	Danio rerio(zebrafish)
Product	ATP-binding cassette, sub-family B (MDR/TAP), member 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR388365.10, EH508856.1 and EH534990.1. On Oct 24, 2015 this sequence version replaced NM_001114583.1. Transcript Variant: This variant (2) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 2 which is shorter than and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC154556.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAAGA AATCCAAACT CAAAGTGTCA AGTGACAAAA AAGAGGAGAA TGGAGATGTA 
AGCGGTGAGA AAAATGGAAA GGAAGAGAAG GAGGAAAAGG AAAAGTTGGA GATGGTGGGA 
CCGATAGAAT TATTTCGATA TGCAGATAGT ATCGACATTT TACTGATGAT GCTTGGCTTA 
ATCATGTCCA TGGCCAATGG GGCAGTTCTT CCTCTCATGG TAATTGTATT TGGAGACATG 
ACGGACAGCT TTGTGGATGA TACATTGTTG GACAATTTGA AGAACATTAC CCTCCCACCT 
AACTTCACCT TTCCAGAGAC CAGTAATATT ACTTTGGGGG AGAAGATGAC AACACATGCT 
ATATACTACA GCATCATGGG ATTTGTGGTC TTGGTGGCGG CATATATGCA GGTGGCCTTT 
TGGACTCTTG CGGCAGGACG TCAGGTGAAG AAACTTAGAA AGATATTCTT CCACTCCATC 
ATGAAACAGG AGATCGGCTG GTTTGATGTC AACGAGACGG GACAACTCAA CACACGCCTT 
ACAGATGATG TTTACAAAAT AAATGAAGGC ATTGGGGATA AACTTGGCAT GCTGATTCAG 
AATCTCACTA CTTTTATTGT TGGCATTATC ATTGGCTTTG CTAAAGGCTG GAAGCTGACC 
CTCGTCATCC TCGCTGTTAG CCCGTTACTC GGTATCTCAG CCGCTGTCAT CGGAAAGGTG 
ATGACAACAT TCACGTCGAA GGAGCAGACG GCCTATGCGA AGGCTGGAGC AGTGGCAGAG 
GAAGTGCTGT CATCCATCAG AACCGTCTTT GCCTTTGGTG GACAGAAGAA AGAGATCAAG 
AGGTATCACA AAAACTTAGA GGATGCTAAG AACGTTGGAG TAAGGAAGGC CATAACTGTG 
AACATTGCTA TGGGCTTCAC ATTCTTCATG ATCTACATGT CCTATGCACT GGCCTTTTGG 
TACGGCAGCA CTCTTATCCT GGGTGGGGAA TATACGATTG GCATGCTTTT GACGATTTTC 
TTCGCTGTCC TAATTGGAGC TTTTGGCCTC GGTCAAACGT CTCCAAACAT ACAGACCTTC 
TCCAGTGCTA GAGGAGCCGC CCATAAAGTT TTCCAGATCA TTGATCACGA ACCCAAGATT 
AACAGTTTCT CTGAGGAAGG ATACAAACTT GACGTCGTTA AAGGAAACAT TGAGTTCAAA 
AACATTCATT TCAGATACCC CTCGCGAGAT GATGTGAAGG TCCTGAATGG CATGAATCTT 
AAAGTCATGA GTGGACAGAC CATTGCTTTA GTGGGCAGCA GTGGGTGTGG AAAAAGCACA 
ACAATCCAGC TGCTGCAACG CTTCTACGAC CCACAGGAAG GGAGCGTTTC TATAGATGGT 
CATGATATTC GCTCTCTGAA TGTGAGAGGT CTGCGAGAAC TGATTGGCGT GGTCAGTCAA 
GAACCAGTGC TGTTTGCAAC AACAATTGCC GAAAACATCC GCTACGGCCG GCAAGACGTC 
ACCCAGGATG AGATTGAGCA GGCTGCACGT GAAGCCAATG CCTACAACTT CATCATGAAG 
CTCCCTGATA AATTTGAGAC GCTGGTTGGA GACAGGGGAA CCCAGATGAG TGGCGGACAA 
AAGCAGCGTA TTGCCATTGC ACGAGCACTT GTGCGCAACC CAAAAATCCT TCTTCTCGAC 
GAGGCTACCT CTGCCCTGGA TGCAGAGAGT GAGACTATTG TGCAAGCAGC TCTAGATAAG 
GTGAGGCTGG GAAGGACCAC CATAGTTGTG GCTCACCGAC TCTCTACTAT TCGAAATGCT 
GATGTCATCG CTGGTTTCCA GAATGGTGAA ATAGTAGAAC TGGGCACGCA TGATGAACTG 
ATGGAAAGGA AAGGAATCTA CCACTCTCTG GTTAATATGC AGGTTAGAAA CCCACTGTTC 
AGACATGAAT CATTTGCAGC TAGTACGCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108055.2
CDS	117..2069
Translation	MGKKSKLKVSSDKKEENGDVSGEKNGKEEKEEKEKLEMVGPIELFRYADSIDILLMMLGLIMSMANGAVLPLMVIVFGDMTDSFVDDTLLDNLKNITLPPNFTFPETSNITLGEKMTTHAIYYSIMGFVVLVAAYMQVAFWTLAAGRQVKKLRKIFFHSIMKQEIGWFDVNETGQLNTRLTDDVYKINEGIGDKLGMLIQNLTTFIVGIIIGFAKGWKLTLVILAVSPLLGISAAVIGKVMTTFTSKEQTAYAKAGAVAEEVLSSIRTVFAFGGQKKEIKRYHKNLEDAKNVGVRKAITVNIAMGFTFFMIYMSYALAFWYGSTLILGGEYTIGMLLTIFFAVLIGAFGLGQTSPNIQTFSSARGAAHKVFQIIDHEPKINSFSEEGYKLDVVKGNIEFKNIHFRYPSRDDVKVLNGMNLKVMSGQTIALVGSSGCGKSTTIQLLQRFYDPQEGSVSIDGHDIRSLNVRGLRELIGVVSQEPVLFATTIAENIRYGRQDVTQDEIEQAAREANAYNFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQAALDKVRLGRTTIVVAHRLSTIRNADVIAGFQNGEIVELGTHDELMERKGIYHSLVNMQVRNPLFRHESFAASTL

Target ORF information:

RefSeq Version	NM_001114583.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATP-binding cassette, sub-family B (MDR/TAP), member 4 (abcb4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114583.2

ORF Insert Sequence:

ATGGGCAAGA AATCCAAACT CAAAGTGTCA AGTGACAAAA AAGAGGAGAA TGGAGATGTA 
AGCGGTGAGA AAAATGGAAA GGAAGAGAAG GAGGAAAAGG AAAAGTTGGA GATGGTGGGA 
CCGATAGAAT TATTTCGATA TGCAGATAGT ATCGACATTT TACTGATGAT GCTTGGCTTA 
ATCATGTCCA TGGCCAATGG GGCAGTTCTT CCTCTCATGG TAATTGTATT TGGAGACATG 
ACGGACAGCT TTGTGGATGA TACATTGTTG GACAATTTGA AGAACATTAC CCTCCCACCT 
AACTTCACCT TTCCAGAGAC CAGTAATATT ACTTTGGGGG AGAAGATGAC AACACATGCT 
ATATACTACA GCATCATGGG ATTTGTGGTC TTGGTGGCGG CATATATGCA GGTGGCCTTT 
TGGACTCTTG CGGCAGGACG TCAGGTGAAG AAACTTAGAA AGATATTCTT CCACTCCATC 
ATGAAACAGG AGATCGGCTG GTTTGATGTC AACGAGACGG GACAACTCAA CACACGCCTT 
ACAGATGATG TTTACAAAAT AAATGAAGGC ATTGGGGATA AACTTGGCAT GCTGATTCAG 
AATCTCACTA CTTTTATTGT TGGCATTATC ATTGGCTTTG CTAAAGGCTG GAAGCTGACC 
CTCGTCATCC TCGCTGTTAG CCCGTTACTC GGTATCTCAG CCGCTGTCAT CGGAAAGGTG 
ATGACAACAT TCACGTCGAA GGAGCAGACG GCCTATGCGA AGGCTGGAGC AGTGGCAGAG 
GAAGTGCTGT CATCCATCAG AACCGTCTTT GCCTTTGGTG GACAGAAGAA AGAGATCAAG 
AGGTATCACA AAAACTTAGA GGATGCTAAG AACGTTGGAG TAAGGAAGGC CATAACTGTG 
AACATTGCTA TGGGCTTCAC ATTCTTCATG ATCTACATGT CCTATGCACT GGCCTTTTGG 
TACGGCAGCA CTCTTATCCT GGGTGGGGAA TATACGATTG GCATGCTTTT GACGATTTTC 
TTCGCTGTCC TAATTGGAGC TTTTGGCCTC GGTCAAACGT CTCCAAACAT ACAGACCTTC 
TCCAGTGCTA GAGGAGCCGC CCATAAAGTT TTCCAGATCA TTGATCACGA ACCCAAGATT 
AACAGTTTCT CTGAGGAAGG ATACAAACTT GACGTCGTTA AAGGAAACAT TGAGTTCAAA 
AACATTCATT TCAGATACCC CTCGCGAGAT GATGTGAAGG TCCTGAATGG CATGAATCTT 
AAAGTCATGA GTGGACAGAC CATTGCTTTA GTGGGCAGCA GTGGGTGTGG AAAAAGCACA 
ACAATCCAGC TGCTGCAACG CTTCTACGAC CCACAGGAAG GGAGCGTTTC TATAGATGGT 
CATGATATTC GCTCTCTGAA TGTGAGAGGT CTGCGAGAAC TGATTGGCGT GGTCAGTCAA 
GAACCAGTGC TGTTTGCAAC AACAATTGCC GAAAACATCC GCTACGGCCG GCAAGACGTC 
ACCCAGGATG AGATTGAGCA GGCTGCACGT GAAGCCAATG CCTACAACTT CATCATGAAG 
CTCCCTGATA AATTTGAGAC GCTGGTTGGA GACAGGGGAA CCCAGATGAG TGGCGGACAA 
AAGCAGCGTA TTGCCATTGC ACGAGCACTT GTGCGCAACC CAAAAATCCT TCTTCTCGAC 
GAGGCTACCT CTGCCCTGGA TGCAGAGAGT GAGACTATTG TGCAAGCAGC TCTAGATAAG 
GTGAGGCTGG GAAGGACCAC CATAGTTGTG GCTCACCGAC TCTCTACTAT TCGAAATGCT 
GATGTCATCG CTGGTTTCCA GAATGGTGAA ATAGTAGAAC TGGGCACGCA TGATGAACTG 
ATGGAAAGGA AAGGAATCTA CCACTCTCTG GTTAATATGC AGGTTAGAAA CCCACTGTTC 
AGACATGAAT CATTTGCAGC TAGTACGCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

abcb4 ( NM_001316714.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001303643.1 Danio rerio ATP-binding cassette, sub-family B (MDR/TAP), member 4 (abcb4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa21533
Clone ID Related Accession (Same CDS sequence)	NM_001316714.1
Accession Version	NM_001316714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576771200000
Organism	Danio rerio(zebrafish)
Product	ATP-binding cassette, sub-family B (MDR/TAP), member 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR388365.10, EH508856.1, EH534990.1, BX322614.3, CF999615.1 and DY562772.1. On Oct 24, 2015 this sequence version replaced XM_005158038.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01021829.1, GDQH01000079.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAAGA AATCCAAACT CAAAGTGTCA AGTGACAAAA AAGAGGAGAA TGGAGATGTA 
AGCGGTGAGA AAAATGGAAA GGAAGAGAAG GAGGAAAAGG AAAAGTTGGA GATGGTGGGA 
CCGATAGAAT TATTTCGATA TGCAGATAGT ATCGACATTT TACTGATGAT GCTTGGCTTA 
ATCATGTCCA TGGCCAATGG GGCAGTTCTT CCTCTCATGG TAATTGTATT TGGAGACATG 
ACGGACAGCT TTGTGGATGA TACATTGTTG GACAATTTGA AGAACATTAC CCTCCCACCT 
AACTTCACCT TTCCAGAGAC CAGTAATATT ACTTTGGGGG AGAAGATGAC AACACATGCT 
ATATACTACA GCATCATGGG ATTTGTGGTC TTGGTGGCGG CATATATGCA GGTGGCCTTT 
TGGACTCTTG CGGCAGGACG TCAGGTGAAG AAACTTAGAA AGATATTCTT CCACTCCATC 
ATGAAACAGG AGATCGGCTG GTTTGATGTC AACGAGACGG GACAACTCAA CACACGCCTT 
ACAGATGATG TTTACAAAAT AAATGAAGGC ATTGGGGATA AACTTGGCAT GCTGATTCAG 
AATCTCACTA CTTTTATTGT TGGCATTATC ATTGGCTTTG CTAAAGGCTG GAAGCTGACC 
CTCGTCATCC TCGCTGTTAG CCCGTTACTC GGTATCTCAG CCGCTGTCAT CGGAAAGGTG 
ATGACAACAT TCACGTCGAA GGAGCAGACG GCCTATGCGA AGGCTGGAGC AGTGGCAGAG 
GAAGTGCTGT CATCCATCAG AACCGTCTTT GCCTTTGGTG GACAGAAGAA AGAGATCAAG 
AGGTATCACA AAAACTTAGA GGATGCTAAG AACGTTGGAG TAAGGAAGGC CATAACTGTG 
AACATTGCTA TGGGCTTCAC ATTCTTCATG ATCTACATGT CCTATGCACT GGCCTTTTGG 
TACGGCAGCA CTCTTATCCT GGGTGGGGAA TATACGATTG GCATGCTTTT GACGATTTTC 
TTCGCTGTCC TAATTGGAGC TTTTGGCCTC GGTCAAACGT CTCCAAACAT ACAGACCTTC 
TCCAGTGCTA GAGGAGCCGC CCATAAAGTT TTCCAGATCA TTGATCACGA ACCCAAGATT 
AACAGTTTCT CTGAGGAAGG ATACAAACTT GACGTCGTTA AAGGAAACAT TGAGTTCAAA 
AACATTCATT TCAGATACCC CTCGCGAGAT GATGTGAAGG TCCTGAATGG CATGAATCTT 
AAAGTCATGA GTGGACAGAC CATTGCTTTA GTGGGCAGCA GTGGGTGTGG AAAAAGCACA 
ACAATCCAGC TGCTGCAACG CTTCTACGAC CCACAGGAAG GGAGCGTTTC TATAGATGGT 
CATGATATTC GCTCTCTGAA TGTGAGAGGT CTGCGAGAAC TGATTGGCGT GGTCAGTCAA 
GAACCAGTGC TGTTTGCAAC AACAATTGCC GAAAACATCC GCTACGGCCG GCAAGACGTC 
ACCCAGGATG AGATTGAGCA GGCTGCACGT GAAGCCAATG CCTACAACTT CATCATGAAG 
CTCCCTGATA AATTTGAGAC GCTGGTTGGA GACAGGGGAA CCCAGATGAG TGGCGGACAA 
AAGCAGCGTA TTGCCATTGC ACGAGCACTT GTGCGCAACC CAAAAATCCT TCTTCTCGAC 
GAGGCTACCT CTGCCCTGGA TGCAGAGAGT GAGACTATTG TGCAAGCAGC TCTAGATAAG 
GTGAGGCTGG GAAGGACCAC CATAGTTGTG GCTCACCGAC TCTCTACTAT TCGAAATGCT 
GATGTCATCG CTGGTTTCCA GAATGGTGAA ATAGTAGAAC TGGGCACGCA TGATGAACTG 
ATGGAAAGGA AAGGAATCTA CCACTCTCTG GTTAATATGC AGATGTTTAA AAGCACAGAG 
GTAGCAGAGG AGGACAGTGA GGAGATGACT ATGGATGAGA AGAGTCCTTC AGTCAGCTCT 
ATGAATGAAC GCACACTCTT TAGACAGAAA TCCAGAAGTG GCTCCGAAAA AGAGCTGAAG 
GAAGAAGAGA AACCGACAGA GGAAGAAAAG GTTCCAAACG TTTCGTTCCT GACTGTCCTG 
AAACTAAATT ATCCTGAATG GCCATACATG GTGGTCGGAA TTCTCTGTGC CACCATCAAC 
GGAGGCATGC AGCCTGCTTT TGCTGTCATC TTCTCTAAAA TCATTGCAGT GTTTGCTGAG 
CCGGATCAGA ATCTCGTGCG GCAGAGGTGT GACCTCTACT CTCTGCTGTT TGCTGGCATT 
GGAGTTTTGT CCTTTTTCAC TCTTTTCTTA CAGGGTTTCT GTTTTGGCAA AGCTGGCGAG 
CTTCTGACTA TGCGACTGAG GTTTAAAGCC TTCAATGCAA TGATGAGACA GGACCTGGCC 
TGGTATGACG ATACCAAGAA CAGCGTGGGT GCGCTGACCA CCAGACTGGC TGCGGATACT 
GCTCAAGTGC AGGGCGCGAC TGGTGTGAGA TTGGCAACAC TGGCGCAGAA TGTGGCCAAC 
CTAGGCACCG CCATTGTCAT CTCATTTGTG TACGGTTGGC AGCTGACCCT CCTTATCCTC 
TCAATTGTGC CAATCATGGC TGTGGCAGGA GCTATTCAGA TGAAGTTACT GGCAGGACAT 
GCTCTAAAAG ACAAGAAAGA ACTTGAACAA GCTGGAAAGA TTGCAACAGA AGCCATTGAG 
AATGTCCGTA CTGTGGTTTC ATTGACCAGA GAGAGCAAGT TTGAAAGTCT CTATGAGGAG 
AACCTAATTG TGCCCTACAA AAATGCCAAG AAAAAAGCAC ACGTCTTTGG CCTGACGTTC 
TCTTTCTCTC AAGCCATGAT TTATTTTGCC TATGCCGGTT GCTTCAAGTT TGGCTCTTGG 
CTCATTGAAC AAAAGCTGAT GACCTTTGAG GGTGTCTTCC TCGTGATCTC AGCTGTGGTG 
TATGGAGCAA TGGCAGTTGG AGAGGCAAAC TCCTTCACTC CAAACTACGC TAAAGCCAAG 
ATGTCTGCTT CTCACGTCCT GATGCTCATC AACAGAGCCC CGGCCATTGA TAACTCCTCA 
GAGGATGGAG ACAAGCCGGA TAAGTTCGAA GGGAATGTGG GTTTTGAACA TGTGTATTTC 
AAGTACCCAT CCCGGCCGGA TGTGCCAGTG CTGCAGGGTC TCAAGCTTCG GGTGAAGAAG 
GGGCAAACAC TCGCTCTGGT GGGCAGCAGT GGCTGTGGAA AGAGCACCAC CATCCAGTTA 
CTGGAGAGAT TTTATGACCC TCAGCAGGGC AGAGTGATGC TGGATGACAA TGACGCAAAG 
CAGCTGAACA TCCATTGGCT GCGCTCTCAA ATTGGCATCG TTTCCCAGGA GCCTGTGCTG 
TTTGACTGCA GCCTGGCTGA GAACATCGCA TATGGAGATA ACAGCAGAGA AGTGGACCAG 
GAGGAGATCG TAGAGGCCGC TAAAGCTGCA AACATACACT CATTCATAGA GAACCTGCCA 
CAGCGGTACC AGACGCAGGC GGGTGATAAG GGTACGCAGC TCTCAGGAGG ACAGAAGCAG 
CGCATTGCCA TAGCGAGAGC CATCCTCAGG AATCCCAAGG TCCTGCTGCT GGATGAAGCC 
ACGTCTGCTC TGGACACAGA GAGCGAGAAG ATTGTTCAGG ACGCTCTGGA CAAGGCGAGC 
AAGGGCCGCA CATGCATCAT TGTAGCCCAC CGGCTGTCCA CCATCCAGAA TGCAGACTGT 
ATAGCGGTGG TCCAGAACGG GGTGGTGGTA GAGCAGGGCA CGCACCAACA GCTTCTCAGC 
CAGCAGGGGG CGTATTATAC TCTTGTCACC TCACAGATGA GCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303643.1
CDS	117..3944
Translation	MGKKSKLKVSSDKKEENGDVSGEKNGKEEKEEKEKLEMVGPIELFRYADSIDILLMMLGLIMSMANGAVLPLMVIVFGDMTDSFVDDTLLDNLKNITLPPNFTFPETSNITLGEKMTTHAIYYSIMGFVVLVAAYMQVAFWTLAAGRQVKKLRKIFFHSIMKQEIGWFDVNETGQLNTRLTDDVYKINEGIGDKLGMLIQNLTTFIVGIIIGFAKGWKLTLVILAVSPLLGISAAVIGKVMTTFTSKEQTAYAKAGAVAEEVLSSIRTVFAFGGQKKEIKRYHKNLEDAKNVGVRKAITVNIAMGFTFFMIYMSYALAFWYGSTLILGGEYTIGMLLTIFFAVLIGAFGLGQTSPNIQTFSSARGAAHKVFQIIDHEPKINSFSEEGYKLDVVKGNIEFKNIHFRYPSRDDVKVLNGMNLKVMSGQTIALVGSSGCGKSTTIQLLQRFYDPQEGSVSIDGHDIRSLNVRGLRELIGVVSQEPVLFATTIAENIRYGRQDVTQDEIEQAAREANAYNFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQAALDKVRLGRTTIVVAHRLSTIRNADVIAGFQNGEIVELGTHDELMERKGIYHSLVNMQMFKSTEVAEEDSEEMTMDEKSPSVSSMNERTLFRQKSRSGSEKELKEEEKPTEEEKVPNVSFLTVLKLNYPEWPYMVVGILCATINGGMQPAFAVIFSKIIAVFAEPDQNLVRQRCDLYSLLFAGIGVLSFFTLFLQGFCFGKAGELLTMRLRFKAFNAMMRQDLAWYDDTKNSVGALTTRLAADTAQVQGATGVRLATLAQNVANLGTAIVISFVYGWQLTLLILSIVPIMAVAGAIQMKLLAGHALKDKKELEQAGKIATEAIENVRTVVSLTRESKFESLYEENLIVPYKNAKKKAHVFGLTFSFSQAMIYFAYAGCFKFGSWLIEQKLMTFEGVFLVISAVVYGAMAVGEANSFTPNYAKAKMSASHVLMLINRAPAIDNSSEDGDKPDKFEGNVGFEHVYFKYPSRPDVPVLQGLKLRVKKGQTLALVGSSGCGKSTTIQLLERFYDPQQGRVMLDDNDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSREVDQEEIVEAAKAANIHSFIENLPQRYQTQAGDKGTQLSGGQKQRIAIARAILRNPKVLLLDEATSALDTESEKIVQDALDKASKGRTCIIVAHRLSTIQNADCIAVVQNGVVVEQGTHQQLLSQQGAYYTLVTSQMSH

Target ORF information:

RefSeq Version	NM_001316714.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATP-binding cassette, sub-family B (MDR/TAP), member 4 (abcb4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316714.1

ORF Insert Sequence:

ATGGGCAAGA AATCCAAACT CAAAGTGTCA AGTGACAAAA AAGAGGAGAA TGGAGATGTA 
AGCGGTGAGA AAAATGGAAA GGAAGAGAAG GAGGAAAAGG AAAAGTTGGA GATGGTGGGA 
CCGATAGAAT TATTTCGATA TGCAGATAGT ATCGACATTT TACTGATGAT GCTTGGCTTA 
ATCATGTCCA TGGCCAATGG GGCAGTTCTT CCTCTCATGG TAATTGTATT TGGAGACATG 
ACGGACAGCT TTGTGGATGA TACATTGTTG GACAATTTGA AGAACATTAC CCTCCCACCT 
AACTTCACCT TTCCAGAGAC CAGTAATATT ACTTTGGGGG AGAAGATGAC AACACATGCT 
ATATACTACA GCATCATGGG ATTTGTGGTC TTGGTGGCGG CATATATGCA GGTGGCCTTT 
TGGACTCTTG CGGCAGGACG TCAGGTGAAG AAACTTAGAA AGATATTCTT CCACTCCATC 
ATGAAACAGG AGATCGGCTG GTTTGATGTC AACGAGACGG GACAACTCAA CACACGCCTT 
ACAGATGATG TTTACAAAAT AAATGAAGGC ATTGGGGATA AACTTGGCAT GCTGATTCAG 
AATCTCACTA CTTTTATTGT TGGCATTATC ATTGGCTTTG CTAAAGGCTG GAAGCTGACC 
CTCGTCATCC TCGCTGTTAG CCCGTTACTC GGTATCTCAG CCGCTGTCAT CGGAAAGGTG 
ATGACAACAT TCACGTCGAA GGAGCAGACG GCCTATGCGA AGGCTGGAGC AGTGGCAGAG 
GAAGTGCTGT CATCCATCAG AACCGTCTTT GCCTTTGGTG GACAGAAGAA AGAGATCAAG 
AGGTATCACA AAAACTTAGA GGATGCTAAG AACGTTGGAG TAAGGAAGGC CATAACTGTG 
AACATTGCTA TGGGCTTCAC ATTCTTCATG ATCTACATGT CCTATGCACT GGCCTTTTGG 
TACGGCAGCA CTCTTATCCT GGGTGGGGAA TATACGATTG GCATGCTTTT GACGATTTTC 
TTCGCTGTCC TAATTGGAGC TTTTGGCCTC GGTCAAACGT CTCCAAACAT ACAGACCTTC 
TCCAGTGCTA GAGGAGCCGC CCATAAAGTT TTCCAGATCA TTGATCACGA ACCCAAGATT 
AACAGTTTCT CTGAGGAAGG ATACAAACTT GACGTCGTTA AAGGAAACAT TGAGTTCAAA 
AACATTCATT TCAGATACCC CTCGCGAGAT GATGTGAAGG TCCTGAATGG CATGAATCTT 
AAAGTCATGA GTGGACAGAC CATTGCTTTA GTGGGCAGCA GTGGGTGTGG AAAAAGCACA 
ACAATCCAGC TGCTGCAACG CTTCTACGAC CCACAGGAAG GGAGCGTTTC TATAGATGGT 
CATGATATTC GCTCTCTGAA TGTGAGAGGT CTGCGAGAAC TGATTGGCGT GGTCAGTCAA 
GAACCAGTGC TGTTTGCAAC AACAATTGCC GAAAACATCC GCTACGGCCG GCAAGACGTC 
ACCCAGGATG AGATTGAGCA GGCTGCACGT GAAGCCAATG CCTACAACTT CATCATGAAG 
CTCCCTGATA AATTTGAGAC GCTGGTTGGA GACAGGGGAA CCCAGATGAG TGGCGGACAA 
AAGCAGCGTA TTGCCATTGC ACGAGCACTT GTGCGCAACC CAAAAATCCT TCTTCTCGAC 
GAGGCTACCT CTGCCCTGGA TGCAGAGAGT GAGACTATTG TGCAAGCAGC TCTAGATAAG 
GTGAGGCTGG GAAGGACCAC CATAGTTGTG GCTCACCGAC TCTCTACTAT TCGAAATGCT 
GATGTCATCG CTGGTTTCCA GAATGGTGAA ATAGTAGAAC TGGGCACGCA TGATGAACTG 
ATGGAAAGGA AAGGAATCTA CCACTCTCTG GTTAATATGC AGATGTTTAA AAGCACAGAG 
GTAGCAGAGG AGGACAGTGA GGAGATGACT ATGGATGAGA AGAGTCCTTC AGTCAGCTCT 
ATGAATGAAC GCACACTCTT TAGACAGAAA TCCAGAAGTG GCTCCGAAAA AGAGCTGAAG 
GAAGAAGAGA AACCGACAGA GGAAGAAAAG GTTCCAAACG TTTCGTTCCT GACTGTCCTG 
AAACTAAATT ATCCTGAATG GCCATACATG GTGGTCGGAA TTCTCTGTGC CACCATCAAC 
GGAGGCATGC AGCCTGCTTT TGCTGTCATC TTCTCTAAAA TCATTGCAGT GTTTGCTGAG 
CCGGATCAGA ATCTCGTGCG GCAGAGGTGT GACCTCTACT CTCTGCTGTT TGCTGGCATT 
GGAGTTTTGT CCTTTTTCAC TCTTTTCTTA CAGGGTTTCT GTTTTGGCAA AGCTGGCGAG 
CTTCTGACTA TGCGACTGAG GTTTAAAGCC TTCAATGCAA TGATGAGACA GGACCTGGCC 
TGGTATGACG ATACCAAGAA CAGCGTGGGT GCGCTGACCA CCAGACTGGC TGCGGATACT 
GCTCAAGTGC AGGGCGCGAC TGGTGTGAGA TTGGCAACAC TGGCGCAGAA TGTGGCCAAC 
CTAGGCACCG CCATTGTCAT CTCATTTGTG TACGGTTGGC AGCTGACCCT CCTTATCCTC 
TCAATTGTGC CAATCATGGC TGTGGCAGGA GCTATTCAGA TGAAGTTACT GGCAGGACAT 
GCTCTAAAAG ACAAGAAAGA ACTTGAACAA GCTGGAAAGA TTGCAACAGA AGCCATTGAG 
AATGTCCGTA CTGTGGTTTC ATTGACCAGA GAGAGCAAGT TTGAAAGTCT CTATGAGGAG 
AACCTAATTG TGCCCTACAA AAATGCCAAG AAAAAAGCAC ACGTCTTTGG CCTGACGTTC 
TCTTTCTCTC AAGCCATGAT TTATTTTGCC TATGCCGGTT GCTTCAAGTT TGGCTCTTGG 
CTCATTGAAC AAAAGCTGAT GACCTTTGAG GGTGTCTTCC TCGTGATCTC AGCTGTGGTG 
TATGGAGCAA TGGCAGTTGG AGAGGCAAAC TCCTTCACTC CAAACTACGC TAAAGCCAAG 
ATGTCTGCTT CTCACGTCCT GATGCTCATC AACAGAGCCC CGGCCATTGA TAACTCCTCA 
GAGGATGGAG ACAAGCCGGA TAAGTTCGAA GGGAATGTGG GTTTTGAACA TGTGTATTTC 
AAGTACCCAT CCCGGCCGGA TGTGCCAGTG CTGCAGGGTC TCAAGCTTCG GGTGAAGAAG 
GGGCAAACAC TCGCTCTGGT GGGCAGCAGT GGCTGTGGAA AGAGCACCAC CATCCAGTTA 
CTGGAGAGAT TTTATGACCC TCAGCAGGGC AGAGTGATGC TGGATGACAA TGACGCAAAG 
CAGCTGAACA TCCATTGGCT GCGCTCTCAA ATTGGCATCG TTTCCCAGGA GCCTGTGCTG 
TTTGACTGCA GCCTGGCTGA GAACATCGCA TATGGAGATA ACAGCAGAGA AGTGGACCAG 
GAGGAGATCG TAGAGGCCGC TAAAGCTGCA AACATACACT CATTCATAGA GAACCTGCCA 
CAGCGGTACC AGACGCAGGC GGGTGATAAG GGTACGCAGC TCTCAGGAGG ACAGAAGCAG 
CGCATTGCCA TAGCGAGAGC CATCCTCAGG AATCCCAAGG TCCTGCTGCT GGATGAAGCC 
ACGTCTGCTC TGGACACAGA GAGCGAGAAG ATTGTTCAGG ACGCTCTGGA CAAGGCGAGC 
AAGGGCCGCA CATGCATCAT TGTAGCCCAC CGGCTGTCCA CCATCCAGAA TGCAGACTGT 
ATAGCGGTGG TCCAGAACGG GGTGGTGGTA GAGCAGGGCA CGCACCAACA GCTTCTCAGC 
CAGCAGGGGG CGTATTATAC TCTTGTCACC TCACAGATGA GCCACTGA