LOC5579519 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5579519
Entrez Gene ID	5579519
Full Name	blastoderm-specific protein 25D
Synonyms	AAEL014969,AAEL014971
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001647778.1	XP_001647828.1	AAEL014971-PA
XM_021848241.1	XP_021703933.1	blastoderm-specific protein 25D isoform X2
XM_021848239.1	XP_021703931.1	blastoderm-specific protein 25D isoform X1
XM_021848238.1	XP_021703930.1	blastoderm-specific protein 25D isoform X1
XM_021848240.1	XP_021703932.1	blastoderm-specific protein 25D isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5579519 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5579519 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe13909	XM_001647778.1 Latest version!	Aedes aegypti AAEL014971-RA mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OAe124562	XM_021848239.1 Latest version!	Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAe124562	XM_021848240.1 Latest version!	Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAe124562	XM_021848238.1 Latest version!	Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAe124563	XM_021848241.1 Latest version!	Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5579519 ( XM_001647778.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001647828.1 Aedes aegypti AAEL014971-RA mRNA.

Price & Availability↑

CloneID	OAe13909
Clone ID Related Accession (Same CDS sequence)	XM_001647778.1
Accession Version	XM_001647778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL014971-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001837456).

ATGGCCACAT CTACGCCTTG TGTTGCAACA GCAGCAGCAG CTGAAAGTCA AGAACTGGTG 
GATTGTTCCG ACGTGTGCTC GGTAAAATCA GCTCGAAGCA CTCACAGCTT AGCCTCGACG 
CATAGCATTC ATAAATCCGT TTCGCAAACG GACGTCGCCT CGATCAACCA GGAGCAGTTG 
AAACTGCTGG AGCTGCAGGA CGAGATCAAA GAGCTGACCC ACCAGCGAGA CTGTCTGAAG 
ATGGAGTTCC AGCAGTTGAA CGAAGTGAAG CCGATATTGG CGCGCAGCTA CTCCAAAACG 
GCGGCCCATC CAAATCTCAC GCAGAAGGTT CAGCACCTGG AGCAGAAGAA CCGGCAGCTG 
CATGCGATGA TGAAACAACA GCAGCACCAC TCGGAGCAGA TTCTGGAACG AATCTGGATC 
CAACAGCGCA GCGAGATCGC CGAGCTGCGC AACCAACTCG ACGCCCAGAA CATGATAATC 
GCCGACCAAG CGTCCCGACT GGCCAACAAC GACCAGCTGG TGCGGGAACT GTACGTGGAA 
AATGGCCGAC TAGTGGCGCA GGTCCAACGC CTGGAACAGC AAAAAGCCCG GGCTACCATG 
CTGCAGCTTT CGCAGGCCAC TTCCGCCGGT GGCCACTGCC ATGCGGGCCA GCTGAGCGGA 
TTCGTACCTG GGATGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001647828.1
CDS	76..756
Translation	MATSTPCVATAAAAESQELVDCSDVCSVKSARSTHSLASTHSIHKSVSQTDVASINQEQLKLLELQDEIKELTHQRDCLKMEFQQLNEVKPILARSYSKTAAHPNLTQKVQHLEQKNRQLHAMMKQQQHHSEQILERIWIQQRSEIAELRNQLDAQNMIIADQASRLANNDQLVRELYVENGRLVAQVQRLEQQKARATMLQLSQATSAGGHCHAGQLSGFVPGMP

Target ORF information:

RefSeq Version	XM_001647778.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL014971-RA mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001647778.1

ORF Insert Sequence:

ATGGCCACAT CTACGCCTTG TGTTGCAACA GCAGCAGCAG CTGAAAGTCA AGAACTGGTG 
GATTGTTCCG ACGTGTGCTC GGTAAAATCA GCTCGAAGCA CTCACAGCTT AGCCTCGACG 
CATAGCATTC ATAAATCCGT TTCGCAAACG GACGTCGCCT CGATCAACCA GGAGCAGTTG 
AAACTGCTGG AGCTGCAGGA CGAGATCAAA GAGCTGACCC ACCAGCGAGA CTGTCTGAAG 
ATGGAGTTCC AGCAGTTGAA CGAAGTGAAG CCGATATTGG CGCGCAGCTA CTCCAAAACG 
GCGGCCCATC CAAATCTCAC GCAGAAGGTT CAGCACCTGG AGCAGAAGAA CCGGCAGCTG 
CATGCGATGA TGAAACAACA GCAGCACCAC TCGGAGCAGA TTCTGGAACG AATCTGGATC 
CAACAGCGCA GCGAGATCGC CGAGCTGCGC AACCAACTCG ACGCCCAGAA CATGATAATC 
GCCGACCAAG CGTCCCGACT GGCCAACAAC GACCAGCTGG TGCGGGAACT GTACGTGGAA 
AATGGCCGAC TAGTGGCGCA GGTCCAACGC CTGGAACAGC AAAAAGCCCG GGCTACCATG 
CTGCAGCTTT CGCAGGCCAC TTCCGCCGGT GGCCACTGCC ATGCGGGCCA GCTGAGCGGA 
TTCGTACCTG GGATGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579519 ( XM_021848239.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021703931.1 Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAe124562
Clone ID Related Accession (Same CDS sequence)	XM_021848239.1 , XM_021848238.1 , XM_021848240.1
Accession Version	XM_021848239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	blastoderm-specific protein 25D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTTT CGACGGATCC GTACGAGCAG AAGCTGTACA GCTTGTTCCA GGAACACGAT 
GTCCATTCGC GGGGAAAGCT GGGCCGGGAA GCGCTGTTGA AGCTGTGCAA GACGTTGGAG 
TTGGCGAAAC CGACCGAGTT GCTGAAACGG CTGCTACGTG ACGGCAGCAC CAGTCAGGTT 
TCGTTCGGGG AATTCCGTGA AGGATTGCTG TTCATACTGA GCGGCGAGGA GCCGGAGGAA 
TCGATGTCAT CGTCGACGGC AACCGGGGCA CCGGGCCAGC AGCAACAGCA GCATGACCCG 
CCACGAACGG TGCCGGGTCA GAAAGATTCA TCCGAGGGAC AGAATGATGT GGCTACAGGT 
TTACCTACAG TTGCATCTCC GAGTGCAGTC TCCAACACCA GCAGAGTCGG GTCGCCATCG 
CAAGAGACGA CGACACCCTC GGGTAGCATA ATGTCCAACA CATCGAAACG TCCTCACAAA 
TTTACCTACC GCCGAGAAAA CGACCAGTCA ATTGCGGGCG AGAGCGAAGA CATCACATCT 
CTGCAGCCAT CAGTCGTCAA GATAATCGGC TGCAGCAGTA GCAATAACAA TACCAGCCGC 
CAAGTGCCAT CGGGTGAGAG TGAGGACGAT CTCGTACAGA CAACGACCGG CCCTGCAACC 
CCTACCACCA CATTGTATGA TAACGAAAAT GAGTCATCCG ATCGGGAAGT GTCGCCCAAG 
TTTGTAGTCG GATCGAAAAA GTACGGTCGT CGGTCACGTC CCCGCGAGGA CTGCAACGGA 
CTGGACACTT CATCGGATTT CAGCAACTCG GACAACGAAT CGGTCCCCCA ACAACAACGT 
CAGCTTCAGC AAACGGTTCC ACCCTCCTCA GCGGAAGCAG ACAACTCCTC AAGTAAAAAC 
TCCTCCTCCA AAGTGCAACG ATCCGCCTCG CAGAGTGACA TTCATGGCTC AAAGCGACGG 
CGGCCTATGA GCGGAAGCAG GCTTAAGCGT TGCGCATCGC TACCTACGCA TCGACAGCGA 
CCGGATCTAG GCAAACTGAA CTCGAAGAAC GTACGCGATA AGCTCAACAT GGATGATAAG 
ATCATCGACA AGAAGATCAA CTTCATGAAA CTTTCGGAGA ATTTGCGGGA CATTTGGGAT 
ACTCTAGTGG GAAGCGGCCA CGGAGATCTA CTTAATCAAT CACAGCTGGA GGTCGTCTGT 
GAACGAGTAG GTCTACAAAA ACTACCCGCC AAGCTGGCCG CTCGGGAAGT TTTCGCGAAA 
TTGTCTCTCC AATCGATCGA GGGAATCAGC TACGATGATT TTATTTCGCT GCTGCAAAGC 
GATTCGGATA TTCTTGCCAT TGGCGGCGGC GGCGGCGGCG AAAAGGACCC GACAGGTGGC 
GGTGATAATG GCGTTCAGCG ATTGCGATTG TTCGACGAGA GCGACAGCTT CAAGGTTCAG 
GAACTGGATC GCGACAGCGT GGAGGAAACG GTTGGCTTCA CCGTGCGAAT GCCAGCCGAG 
TGGACAACCG AGATCGGATC ACTCTCGACG GCCATCATCA TCGACATGTG GGAGTCGGCC 
GGAATCAGCG AGCCGCGAGC TTTGCTGCAG GATCTCGGCT TCTACGGGCT GGAGGTGCAG 
GTAACGGAGC TAATCGCAGC ACTGGAGGAC GAACAGCAAC GGCTCGGCGA TGGCAACGAC 
ACCTCTGTGG TGGTGAGGGC ATCGCTGGCG CTTCACAAGG CGGAAGTCAG TGCCCTGCGA 
CAGGCGTTCC TTCAGCTGAT GGAAGAAAAC AAGAAACTCT ACTCCGACAA CAAAGAAGCC 
AATCAACGCG CCCAACTGCT GCTTCAGGAG GTGGACGAGC GGCACAACTC GTTGGAAAGT 
GCCACCCGGG ACAAAATCCG TCTCCTGGAG CAGCGTCACC ATGAAACGGT GAAAGAGCTG 
ACGTCGCAAC TGGCGTTTGA GAGGGAGCAA TTGTCCTGTG CGAATGCCAC CTTGGAGAAA 
CGGATCCAAA GCCTGGAACA GGACGAGAGC AAGGTGAAAG CGGAGATGAA CTCGCTGCTG 
GAGGAGAACG AGGGTTTGAG GGCTGAGCAG GAGAACCTCA CCAAGGAGCT GACGGATATT 
CTAGAGAAGA ATATCAAGCT CAATAAAGAT ATTGCCGAGC TGGAGGAAAA CAATCGCAAC 
GACTTCGATG ATGAATATGC CGGGAAGAAC GACCTGGAAG TTTTGGAGTT GATCGAGAAG 
AATTCTCTGT TGCAAGATAA AAATAAAGAA CTGAAAGATA AGAATGATGA GTTGAGTGTG 
GAGATTGAAA TGTTGAATGC CGAGCTGGCG AAGTTCAAAT TGAAGCGAAG TGGTGTTAGC 
AGGACAAGCT CGTTGGATGT GTCGCCGGTG ATGGTCGAAT CGACTGGGTG CGAAGAGAAT 
ACAAGTGCTG CTAGAAAGCG TCAAGGAGAT TCCCCAAGCA AGGCGAGACT CTCAGATGAA 
AGTCCCCGGT TGGGAAAATT CAGGAAGTGC AGCAACGATG TGGAGAATGA GTCCGATTCG 
AGTGGGGATT GGATGGCTCT GCATTCCGAG CTTGCACTGA AAGTGGAAGC TACCTCAGGT 
ATTAGTGTAG AAAAAACACA GGCAGAAGAA ATACAGCAAC TAAAGGCGAA AGTTGCTGAA 
CTAGAGGAGT TGAATAAGAA GAAAGAGGAA GAGAGTACTG GTTTTGAAAA GAGTGATTTG 
GAGAAAGTGT CCGATGGTAG TGATACAACA CAGTCCGAAA TGGCGAAAGT GAAGGCACGT 
TGCGAGGAGT TGGAAGCAAG TTTAGAACAA TTGAGCAAAG AATACGAAGC TTGTGAAGAC 
TACTGGCAGA GTAAGGTGAA CGAAGAAAGA TTGCTGTATG AAGAGGAACA GCGAATTGCC 
GACGAAAAAC TGAACGAGCT GTGTAAAAAG GTGGCCGAAT ACGAGGAGCA GTTTGCCGGC 
CAAGGTGGTA ATAAAAATGA TGATGGCCGA CTATCACCTA TCGAGGAGAA AGATGTCCTG 
GAGCAGCAGT ATCTGGATTT GGAGGTGGAA TTCCAACAGG TGCAGACTAT GCTGCAAGAA 
AAGACGCATG AACTGGAAAA GCTGCGGGGC AGGATCCTGG AGCTAGAACA ACTCGTCAAA 
TATCCATCGG AGGCGTTATT AGCTACGCCA CCACGTGTTG ATACGCCTGA AATGGAGAGC 
CCCGCCAGTT CACCAATCAG CTATCTATGG AATCAGAGCA CCATCCAAGC TCCAGCCACA 
CGGGACTACC AGAATCCGAA CTGGTGCAAG CCACTGAAAG CGAGCAGTAT TCCGCTTGAA 
ACGAATCTTG TTAATAACGA TGTGAACGAA TCGTTACCTC AATCCAAAAT GGCTGAAGAA 
CTGAAAGGCG ATGCCGACGA CTCCGCATTC GATCGTACGA TATCCCCAAT TCAGAAACCT 
ATCTCGAAAG AACTTATGGC CACATCTACG CCTTGTGTTG CAACAGCAGC AGCAGCTGAA 
AGTCAAGAAC TGGTGGATTG TTCCGACGTG TGCTCGGTAA AATCAGCTCG AAGCACTCAC 
AGTTTAGCCT CCACGCATAG CATTCACAAA TCCGTTTCAC AAACGGACGT CGCCTCGATC 
AACCAGGAGC AGTTGAAACT GCTGGAGCTG CAGGACGAGA TCAAAGAGCT GACCCACCAG 
CGAGACTGTC TGAAGATGGA GTTCCAGCAG TTGAACGAAG TGAAGCCGAT ATTGGCGCGG 
AGCTACTCCA AAACGGCGGC TCATCCAAAC CTCACGCAGA AGGTTCAGCA CCTGGAGCAG 
AAGAACCGGC AGCTGCATGC GATGATGAAA CAACAGCAGC ACCACTCGGA GCAGATTCTG 
GAACGAATCT GGATCCAACA GCGCAGCGAG ATCGCCGAGC TGCGCAATCA ACTCGACGCC 
CAGAACATGA TAATCGCCGA CCAAGCGTCC CGACTGGCCA ACAACGACCA GCTGGTGCGG 
GAACTGTACG TGGAAAATGG CCGACTAGTG GCGCAGGTCC AACGCCTGGA ACAGCAAAAG 
GCCCGGGCTA CCATGCTGCA GCTTTCGCAG GCCACTTCCG CCGGTGGCCA CTGCCATGCG 
GGCCAGCTGA GCGGATTCGT ACCTGGGATG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021703931.1
CDS	1029..5144
Translation	MALSTDPYEQKLYSLFQEHDVHSRGKLGREALLKLCKTLELAKPTELLKRLLRDGSTSQVSFGEFREGLLFILSGEEPEESMSSSTATGAPGQQQQQHDPPRTVPGQKDSSEGQNDVATGLPTVASPSAVSNTSRVGSPSQETTTPSGSIMSNTSKRPHKFTYRRENDQSIAGESEDITSLQPSVVKIIGCSSSNNNTSRQVPSGESEDDLVQTTTGPATPTTTLYDNENESSDREVSPKFVVGSKKYGRRSRPREDCNGLDTSSDFSNSDNESVPQQQRQLQQTVPPSSAEADNSSSKNSSSKVQRSASQSDIHGSKRRRPMSGSRLKRCASLPTHRQRPDLGKLNSKNVRDKLNMDDKIIDKKINFMKLSENLRDIWDTLVGSGHGDLLNQSQLEVVCERVGLQKLPAKLAAREVFAKLSLQSIEGISYDDFISLLQSDSDILAIGGGGGGEKDPTGGGDNGVQRLRLFDESDSFKVQELDRDSVEETVGFTVRMPAEWTTEIGSLSTAIIIDMWESAGISEPRALLQDLGFYGLEVQVTELIAALEDEQQRLGDGNDTSVVVRASLALHKAEVSALRQAFLQLMEENKKLYSDNKEANQRAQLLLQEVDERHNSLESATRDKIRLLEQRHHETVKELTSQLAFEREQLSCANATLEKRIQSLEQDESKVKAEMNSLLEENEGLRAEQENLTKELTDILEKNIKLNKDIAELEENNRNDFDDEYAGKNDLEVLELIEKNSLLQDKNKELKDKNDELSVEIEMLNAELAKFKLKRSGVSRTSSLDVSPVMVESTGCEENTSAARKRQGDSPSKARLSDESPRLGKFRKCSNDVENESDSSGDWMALHSELALKVEATSGISVEKTQAEEIQQLKAKVAELEELNKKKEEESTGFEKSDLEKVSDGSDTTQSEMAKVKARCEELEASLEQLSKEYEACEDYWQSKVNEERLLYEEEQRIADEKLNELCKKVAEYEEQFAGQGGNKNDDGRLSPIEEKDVLEQQYLDLEVEFQQVQTMLQEKTHELEKLRGRILELEQLVKYPSEALLATPPRVDTPEMESPASSPISYLWNQSTIQAPATRDYQNPNWCKPLKASSIPLETNLVNNDVNESLPQSKMAEELKGDADDSAFDRTISPIQKPISKELMATSTPCVATAAAAESQELVDCSDVCSVKSARSTHSLASTHSIHKSVSQTDVASINQEQLKLLELQDEIKELTHQRDCLKMEFQQLNEVKPILARSYSKTAAHPNLTQKVQHLEQKNRQLHAMMKQQQHHSEQILERIWIQQRSEIAELRNQLDAQNMIIADQASRLANNDQLVRELYVENGRLVAQVQRLEQQKARATMLQLSQATSAGGHCHAGQLSGFVPGMP

Target ORF information:

RefSeq Version	XM_021848239.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848239.1

ORF Insert Sequence:

ATGGCCCTTT CGACGGATCC GTACGAGCAG AAGCTGTACA GCTTGTTCCA GGAACACGAT 
GTCCATTCGC GGGGAAAGCT GGGCCGGGAA GCGCTGTTGA AGCTGTGCAA GACGTTGGAG 
TTGGCGAAAC CGACCGAGTT GCTGAAACGG CTGCTACGTG ACGGCAGCAC CAGTCAGGTT 
TCGTTCGGGG AATTCCGTGA AGGATTGCTG TTCATACTGA GCGGCGAGGA GCCGGAGGAA 
TCGATGTCAT CGTCGACGGC AACCGGGGCA CCGGGCCAGC AGCAACAGCA GCATGACCCG 
CCACGAACGG TGCCGGGTCA GAAAGATTCA TCCGAGGGAC AGAATGATGT GGCTACAGGT 
TTACCTACAG TTGCATCTCC GAGTGCAGTC TCCAACACCA GCAGAGTCGG GTCGCCATCG 
CAAGAGACGA CGACACCCTC GGGTAGCATA ATGTCCAACA CATCGAAACG TCCTCACAAA 
TTTACCTACC GCCGAGAAAA CGACCAGTCA ATTGCGGGCG AGAGCGAAGA CATCACATCT 
CTGCAGCCAT CAGTCGTCAA GATAATCGGC TGCAGCAGTA GCAATAACAA TACCAGCCGC 
CAAGTGCCAT CGGGTGAGAG TGAGGACGAT CTCGTACAGA CAACGACCGG CCCTGCAACC 
CCTACCACCA CATTGTATGA TAACGAAAAT GAGTCATCCG ATCGGGAAGT GTCGCCCAAG 
TTTGTAGTCG GATCGAAAAA GTACGGTCGT CGGTCACGTC CCCGCGAGGA CTGCAACGGA 
CTGGACACTT CATCGGATTT CAGCAACTCG GACAACGAAT CGGTCCCCCA ACAACAACGT 
CAGCTTCAGC AAACGGTTCC ACCCTCCTCA GCGGAAGCAG ACAACTCCTC AAGTAAAAAC 
TCCTCCTCCA AAGTGCAACG ATCCGCCTCG CAGAGTGACA TTCATGGCTC AAAGCGACGG 
CGGCCTATGA GCGGAAGCAG GCTTAAGCGT TGCGCATCGC TACCTACGCA TCGACAGCGA 
CCGGATCTAG GCAAACTGAA CTCGAAGAAC GTACGCGATA AGCTCAACAT GGATGATAAG 
ATCATCGACA AGAAGATCAA CTTCATGAAA CTTTCGGAGA ATTTGCGGGA CATTTGGGAT 
ACTCTAGTGG GAAGCGGCCA CGGAGATCTA CTTAATCAAT CACAGCTGGA GGTCGTCTGT 
GAACGAGTAG GTCTACAAAA ACTACCCGCC AAGCTGGCCG CTCGGGAAGT TTTCGCGAAA 
TTGTCTCTCC AATCGATCGA GGGAATCAGC TACGATGATT TTATTTCGCT GCTGCAAAGC 
GATTCGGATA TTCTTGCCAT TGGCGGCGGC GGCGGCGGCG AAAAGGACCC GACAGGTGGC 
GGTGATAATG GCGTTCAGCG ATTGCGATTG TTCGACGAGA GCGACAGCTT CAAGGTTCAG 
GAACTGGATC GCGACAGCGT GGAGGAAACG GTTGGCTTCA CCGTGCGAAT GCCAGCCGAG 
TGGACAACCG AGATCGGATC ACTCTCGACG GCCATCATCA TCGACATGTG GGAGTCGGCC 
GGAATCAGCG AGCCGCGAGC TTTGCTGCAG GATCTCGGCT TCTACGGGCT GGAGGTGCAG 
GTAACGGAGC TAATCGCAGC ACTGGAGGAC GAACAGCAAC GGCTCGGCGA TGGCAACGAC 
ACCTCTGTGG TGGTGAGGGC ATCGCTGGCG CTTCACAAGG CGGAAGTCAG TGCCCTGCGA 
CAGGCGTTCC TTCAGCTGAT GGAAGAAAAC AAGAAACTCT ACTCCGACAA CAAAGAAGCC 
AATCAACGCG CCCAACTGCT GCTTCAGGAG GTGGACGAGC GGCACAACTC GTTGGAAAGT 
GCCACCCGGG ACAAAATCCG TCTCCTGGAG CAGCGTCACC ATGAAACGGT GAAAGAGCTG 
ACGTCGCAAC TGGCGTTTGA GAGGGAGCAA TTGTCCTGTG CGAATGCCAC CTTGGAGAAA 
CGGATCCAAA GCCTGGAACA GGACGAGAGC AAGGTGAAAG CGGAGATGAA CTCGCTGCTG 
GAGGAGAACG AGGGTTTGAG GGCTGAGCAG GAGAACCTCA CCAAGGAGCT GACGGATATT 
CTAGAGAAGA ATATCAAGCT CAATAAAGAT ATTGCCGAGC TGGAGGAAAA CAATCGCAAC 
GACTTCGATG ATGAATATGC CGGGAAGAAC GACCTGGAAG TTTTGGAGTT GATCGAGAAG 
AATTCTCTGT TGCAAGATAA AAATAAAGAA CTGAAAGATA AGAATGATGA GTTGAGTGTG 
GAGATTGAAA TGTTGAATGC CGAGCTGGCG AAGTTCAAAT TGAAGCGAAG TGGTGTTAGC 
AGGACAAGCT CGTTGGATGT GTCGCCGGTG ATGGTCGAAT CGACTGGGTG CGAAGAGAAT 
ACAAGTGCTG CTAGAAAGCG TCAAGGAGAT TCCCCAAGCA AGGCGAGACT CTCAGATGAA 
AGTCCCCGGT TGGGAAAATT CAGGAAGTGC AGCAACGATG TGGAGAATGA GTCCGATTCG 
AGTGGGGATT GGATGGCTCT GCATTCCGAG CTTGCACTGA AAGTGGAAGC TACCTCAGGT 
ATTAGTGTAG AAAAAACACA GGCAGAAGAA ATACAGCAAC TAAAGGCGAA AGTTGCTGAA 
CTAGAGGAGT TGAATAAGAA GAAAGAGGAA GAGAGTACTG GTTTTGAAAA GAGTGATTTG 
GAGAAAGTGT CCGATGGTAG TGATACAACA CAGTCCGAAA TGGCGAAAGT GAAGGCACGT 
TGCGAGGAGT TGGAAGCAAG TTTAGAACAA TTGAGCAAAG AATACGAAGC TTGTGAAGAC 
TACTGGCAGA GTAAGGTGAA CGAAGAAAGA TTGCTGTATG AAGAGGAACA GCGAATTGCC 
GACGAAAAAC TGAACGAGCT GTGTAAAAAG GTGGCCGAAT ACGAGGAGCA GTTTGCCGGC 
CAAGGTGGTA ATAAAAATGA TGATGGCCGA CTATCACCTA TCGAGGAGAA AGATGTCCTG 
GAGCAGCAGT ATCTGGATTT GGAGGTGGAA TTCCAACAGG TGCAGACTAT GCTGCAAGAA 
AAGACGCATG AACTGGAAAA GCTGCGGGGC AGGATCCTGG AGCTAGAACA ACTCGTCAAA 
TATCCATCGG AGGCGTTATT AGCTACGCCA CCACGTGTTG ATACGCCTGA AATGGAGAGC 
CCCGCCAGTT CACCAATCAG CTATCTATGG AATCAGAGCA CCATCCAAGC TCCAGCCACA 
CGGGACTACC AGAATCCGAA CTGGTGCAAG CCACTGAAAG CGAGCAGTAT TCCGCTTGAA 
ACGAATCTTG TTAATAACGA TGTGAACGAA TCGTTACCTC AATCCAAAAT GGCTGAAGAA 
CTGAAAGGCG ATGCCGACGA CTCCGCATTC GATCGTACGA TATCCCCAAT TCAGAAACCT 
ATCTCGAAAG AACTTATGGC CACATCTACG CCTTGTGTTG CAACAGCAGC AGCAGCTGAA 
AGTCAAGAAC TGGTGGATTG TTCCGACGTG TGCTCGGTAA AATCAGCTCG AAGCACTCAC 
AGTTTAGCCT CCACGCATAG CATTCACAAA TCCGTTTCAC AAACGGACGT CGCCTCGATC 
AACCAGGAGC AGTTGAAACT GCTGGAGCTG CAGGACGAGA TCAAAGAGCT GACCCACCAG 
CGAGACTGTC TGAAGATGGA GTTCCAGCAG TTGAACGAAG TGAAGCCGAT ATTGGCGCGG 
AGCTACTCCA AAACGGCGGC TCATCCAAAC CTCACGCAGA AGGTTCAGCA CCTGGAGCAG 
AAGAACCGGC AGCTGCATGC GATGATGAAA CAACAGCAGC ACCACTCGGA GCAGATTCTG 
GAACGAATCT GGATCCAACA GCGCAGCGAG ATCGCCGAGC TGCGCAATCA ACTCGACGCC 
CAGAACATGA TAATCGCCGA CCAAGCGTCC CGACTGGCCA ACAACGACCA GCTGGTGCGG 
GAACTGTACG TGGAAAATGG CCGACTAGTG GCGCAGGTCC AACGCCTGGA ACAGCAAAAG 
GCCCGGGCTA CCATGCTGCA GCTTTCGCAG GCCACTTCCG CCGGTGGCCA CTGCCATGCG 
GGCCAGCTGA GCGGATTCGT ACCTGGGATG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579519 ( XM_021848238.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021703930.1 Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X1, mRNA.

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CloneID	OAe124562
Clone ID Related Accession (Same CDS sequence)	XM_021848239.1 , XM_021848238.1 , XM_021848240.1
Accession Version	XM_021848238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	blastoderm-specific protein 25D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTTT CGACGGATCC GTACGAGCAG AAGCTGTACA GCTTGTTCCA GGAACACGAT 
GTCCATTCGC GGGGAAAGCT GGGCCGGGAA GCGCTGTTGA AGCTGTGCAA GACGTTGGAG 
TTGGCGAAAC CGACCGAGTT GCTGAAACGG CTGCTACGTG ACGGCAGCAC CAGTCAGGTT 
TCGTTCGGGG AATTCCGTGA AGGATTGCTG TTCATACTGA GCGGCGAGGA GCCGGAGGAA 
TCGATGTCAT CGTCGACGGC AACCGGGGCA CCGGGCCAGC AGCAACAGCA GCATGACCCG 
CCACGAACGG TGCCGGGTCA GAAAGATTCA TCCGAGGGAC AGAATGATGT GGCTACAGGT 
TTACCTACAG TTGCATCTCC GAGTGCAGTC TCCAACACCA GCAGAGTCGG GTCGCCATCG 
CAAGAGACGA CGACACCCTC GGGTAGCATA ATGTCCAACA CATCGAAACG TCCTCACAAA 
TTTACCTACC GCCGAGAAAA CGACCAGTCA ATTGCGGGCG AGAGCGAAGA CATCACATCT 
CTGCAGCCAT CAGTCGTCAA GATAATCGGC TGCAGCAGTA GCAATAACAA TACCAGCCGC 
CAAGTGCCAT CGGGTGAGAG TGAGGACGAT CTCGTACAGA CAACGACCGG CCCTGCAACC 
CCTACCACCA CATTGTATGA TAACGAAAAT GAGTCATCCG ATCGGGAAGT GTCGCCCAAG 
TTTGTAGTCG GATCGAAAAA GTACGGTCGT CGGTCACGTC CCCGCGAGGA CTGCAACGGA 
CTGGACACTT CATCGGATTT CAGCAACTCG GACAACGAAT CGGTCCCCCA ACAACAACGT 
CAGCTTCAGC AAACGGTTCC ACCCTCCTCA GCGGAAGCAG ACAACTCCTC AAGTAAAAAC 
TCCTCCTCCA AAGTGCAACG ATCCGCCTCG CAGAGTGACA TTCATGGCTC AAAGCGACGG 
CGGCCTATGA GCGGAAGCAG GCTTAAGCGT TGCGCATCGC TACCTACGCA TCGACAGCGA 
CCGGATCTAG GCAAACTGAA CTCGAAGAAC GTACGCGATA AGCTCAACAT GGATGATAAG 
ATCATCGACA AGAAGATCAA CTTCATGAAA CTTTCGGAGA ATTTGCGGGA CATTTGGGAT 
ACTCTAGTGG GAAGCGGCCA CGGAGATCTA CTTAATCAAT CACAGCTGGA GGTCGTCTGT 
GAACGAGTAG GTCTACAAAA ACTACCCGCC AAGCTGGCCG CTCGGGAAGT TTTCGCGAAA 
TTGTCTCTCC AATCGATCGA GGGAATCAGC TACGATGATT TTATTTCGCT GCTGCAAAGC 
GATTCGGATA TTCTTGCCAT TGGCGGCGGC GGCGGCGGCG AAAAGGACCC GACAGGTGGC 
GGTGATAATG GCGTTCAGCG ATTGCGATTG TTCGACGAGA GCGACAGCTT CAAGGTTCAG 
GAACTGGATC GCGACAGCGT GGAGGAAACG GTTGGCTTCA CCGTGCGAAT GCCAGCCGAG 
TGGACAACCG AGATCGGATC ACTCTCGACG GCCATCATCA TCGACATGTG GGAGTCGGCC 
GGAATCAGCG AGCCGCGAGC TTTGCTGCAG GATCTCGGCT TCTACGGGCT GGAGGTGCAG 
GTAACGGAGC TAATCGCAGC ACTGGAGGAC GAACAGCAAC GGCTCGGCGA TGGCAACGAC 
ACCTCTGTGG TGGTGAGGGC ATCGCTGGCG CTTCACAAGG CGGAAGTCAG TGCCCTGCGA 
CAGGCGTTCC TTCAGCTGAT GGAAGAAAAC AAGAAACTCT ACTCCGACAA CAAAGAAGCC 
AATCAACGCG CCCAACTGCT GCTTCAGGAG GTGGACGAGC GGCACAACTC GTTGGAAAGT 
GCCACCCGGG ACAAAATCCG TCTCCTGGAG CAGCGTCACC ATGAAACGGT GAAAGAGCTG 
ACGTCGCAAC TGGCGTTTGA GAGGGAGCAA TTGTCCTGTG CGAATGCCAC CTTGGAGAAA 
CGGATCCAAA GCCTGGAACA GGACGAGAGC AAGGTGAAAG CGGAGATGAA CTCGCTGCTG 
GAGGAGAACG AGGGTTTGAG GGCTGAGCAG GAGAACCTCA CCAAGGAGCT GACGGATATT 
CTAGAGAAGA ATATCAAGCT CAATAAAGAT ATTGCCGAGC TGGAGGAAAA CAATCGCAAC 
GACTTCGATG ATGAATATGC CGGGAAGAAC GACCTGGAAG TTTTGGAGTT GATCGAGAAG 
AATTCTCTGT TGCAAGATAA AAATAAAGAA CTGAAAGATA AGAATGATGA GTTGAGTGTG 
GAGATTGAAA TGTTGAATGC CGAGCTGGCG AAGTTCAAAT TGAAGCGAAG TGGTGTTAGC 
AGGACAAGCT CGTTGGATGT GTCGCCGGTG ATGGTCGAAT CGACTGGGTG CGAAGAGAAT 
ACAAGTGCTG CTAGAAAGCG TCAAGGAGAT TCCCCAAGCA AGGCGAGACT CTCAGATGAA 
AGTCCCCGGT TGGGAAAATT CAGGAAGTGC AGCAACGATG TGGAGAATGA GTCCGATTCG 
AGTGGGGATT GGATGGCTCT GCATTCCGAG CTTGCACTGA AAGTGGAAGC TACCTCAGGT 
ATTAGTGTAG AAAAAACACA GGCAGAAGAA ATACAGCAAC TAAAGGCGAA AGTTGCTGAA 
CTAGAGGAGT TGAATAAGAA GAAAGAGGAA GAGAGTACTG GTTTTGAAAA GAGTGATTTG 
GAGAAAGTGT CCGATGGTAG TGATACAACA CAGTCCGAAA TGGCGAAAGT GAAGGCACGT 
TGCGAGGAGT TGGAAGCAAG TTTAGAACAA TTGAGCAAAG AATACGAAGC TTGTGAAGAC 
TACTGGCAGA GTAAGGTGAA CGAAGAAAGA TTGCTGTATG AAGAGGAACA GCGAATTGCC 
GACGAAAAAC TGAACGAGCT GTGTAAAAAG GTGGCCGAAT ACGAGGAGCA GTTTGCCGGC 
CAAGGTGGTA ATAAAAATGA TGATGGCCGA CTATCACCTA TCGAGGAGAA AGATGTCCTG 
GAGCAGCAGT ATCTGGATTT GGAGGTGGAA TTCCAACAGG TGCAGACTAT GCTGCAAGAA 
AAGACGCATG AACTGGAAAA GCTGCGGGGC AGGATCCTGG AGCTAGAACA ACTCGTCAAA 
TATCCATCGG AGGCGTTATT AGCTACGCCA CCACGTGTTG ATACGCCTGA AATGGAGAGC 
CCCGCCAGTT CACCAATCAG CTATCTATGG AATCAGAGCA CCATCCAAGC TCCAGCCACA 
CGGGACTACC AGAATCCGAA CTGGTGCAAG CCACTGAAAG CGAGCAGTAT TCCGCTTGAA 
ACGAATCTTG TTAATAACGA TGTGAACGAA TCGTTACCTC AATCCAAAAT GGCTGAAGAA 
CTGAAAGGCG ATGCCGACGA CTCCGCATTC GATCGTACGA TATCCCCAAT TCAGAAACCT 
ATCTCGAAAG AACTTATGGC CACATCTACG CCTTGTGTTG CAACAGCAGC AGCAGCTGAA 
AGTCAAGAAC TGGTGGATTG TTCCGACGTG TGCTCGGTAA AATCAGCTCG AAGCACTCAC 
AGTTTAGCCT CCACGCATAG CATTCACAAA TCCGTTTCAC AAACGGACGT CGCCTCGATC 
AACCAGGAGC AGTTGAAACT GCTGGAGCTG CAGGACGAGA TCAAAGAGCT GACCCACCAG 
CGAGACTGTC TGAAGATGGA GTTCCAGCAG TTGAACGAAG TGAAGCCGAT ATTGGCGCGG 
AGCTACTCCA AAACGGCGGC TCATCCAAAC CTCACGCAGA AGGTTCAGCA CCTGGAGCAG 
AAGAACCGGC AGCTGCATGC GATGATGAAA CAACAGCAGC ACCACTCGGA GCAGATTCTG 
GAACGAATCT GGATCCAACA GCGCAGCGAG ATCGCCGAGC TGCGCAATCA ACTCGACGCC 
CAGAACATGA TAATCGCCGA CCAAGCGTCC CGACTGGCCA ACAACGACCA GCTGGTGCGG 
GAACTGTACG TGGAAAATGG CCGACTAGTG GCGCAGGTCC AACGCCTGGA ACAGCAAAAG 
GCCCGGGCTA CCATGCTGCA GCTTTCGCAG GCCACTTCCG CCGGTGGCCA CTGCCATGCG 
GGCCAGCTGA GCGGATTCGT ACCTGGGATG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021703930.1
CDS	809..4924
Translation	MALSTDPYEQKLYSLFQEHDVHSRGKLGREALLKLCKTLELAKPTELLKRLLRDGSTSQVSFGEFREGLLFILSGEEPEESMSSSTATGAPGQQQQQHDPPRTVPGQKDSSEGQNDVATGLPTVASPSAVSNTSRVGSPSQETTTPSGSIMSNTSKRPHKFTYRRENDQSIAGESEDITSLQPSVVKIIGCSSSNNNTSRQVPSGESEDDLVQTTTGPATPTTTLYDNENESSDREVSPKFVVGSKKYGRRSRPREDCNGLDTSSDFSNSDNESVPQQQRQLQQTVPPSSAEADNSSSKNSSSKVQRSASQSDIHGSKRRRPMSGSRLKRCASLPTHRQRPDLGKLNSKNVRDKLNMDDKIIDKKINFMKLSENLRDIWDTLVGSGHGDLLNQSQLEVVCERVGLQKLPAKLAAREVFAKLSLQSIEGISYDDFISLLQSDSDILAIGGGGGGEKDPTGGGDNGVQRLRLFDESDSFKVQELDRDSVEETVGFTVRMPAEWTTEIGSLSTAIIIDMWESAGISEPRALLQDLGFYGLEVQVTELIAALEDEQQRLGDGNDTSVVVRASLALHKAEVSALRQAFLQLMEENKKLYSDNKEANQRAQLLLQEVDERHNSLESATRDKIRLLEQRHHETVKELTSQLAFEREQLSCANATLEKRIQSLEQDESKVKAEMNSLLEENEGLRAEQENLTKELTDILEKNIKLNKDIAELEENNRNDFDDEYAGKNDLEVLELIEKNSLLQDKNKELKDKNDELSVEIEMLNAELAKFKLKRSGVSRTSSLDVSPVMVESTGCEENTSAARKRQGDSPSKARLSDESPRLGKFRKCSNDVENESDSSGDWMALHSELALKVEATSGISVEKTQAEEIQQLKAKVAELEELNKKKEEESTGFEKSDLEKVSDGSDTTQSEMAKVKARCEELEASLEQLSKEYEACEDYWQSKVNEERLLYEEEQRIADEKLNELCKKVAEYEEQFAGQGGNKNDDGRLSPIEEKDVLEQQYLDLEVEFQQVQTMLQEKTHELEKLRGRILELEQLVKYPSEALLATPPRVDTPEMESPASSPISYLWNQSTIQAPATRDYQNPNWCKPLKASSIPLETNLVNNDVNESLPQSKMAEELKGDADDSAFDRTISPIQKPISKELMATSTPCVATAAAAESQELVDCSDVCSVKSARSTHSLASTHSIHKSVSQTDVASINQEQLKLLELQDEIKELTHQRDCLKMEFQQLNEVKPILARSYSKTAAHPNLTQKVQHLEQKNRQLHAMMKQQQHHSEQILERIWIQQRSEIAELRNQLDAQNMIIADQASRLANNDQLVRELYVENGRLVAQVQRLEQQKARATMLQLSQATSAGGHCHAGQLSGFVPGMP

Target ORF information:

RefSeq Version	XM_021848238.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848238.1

ORF Insert Sequence:

ATGGCCCTTT CGACGGATCC GTACGAGCAG AAGCTGTACA GCTTGTTCCA GGAACACGAT 
GTCCATTCGC GGGGAAAGCT GGGCCGGGAA GCGCTGTTGA AGCTGTGCAA GACGTTGGAG 
TTGGCGAAAC CGACCGAGTT GCTGAAACGG CTGCTACGTG ACGGCAGCAC CAGTCAGGTT 
TCGTTCGGGG AATTCCGTGA AGGATTGCTG TTCATACTGA GCGGCGAGGA GCCGGAGGAA 
TCGATGTCAT CGTCGACGGC AACCGGGGCA CCGGGCCAGC AGCAACAGCA GCATGACCCG 
CCACGAACGG TGCCGGGTCA GAAAGATTCA TCCGAGGGAC AGAATGATGT GGCTACAGGT 
TTACCTACAG TTGCATCTCC GAGTGCAGTC TCCAACACCA GCAGAGTCGG GTCGCCATCG 
CAAGAGACGA CGACACCCTC GGGTAGCATA ATGTCCAACA CATCGAAACG TCCTCACAAA 
TTTACCTACC GCCGAGAAAA CGACCAGTCA ATTGCGGGCG AGAGCGAAGA CATCACATCT 
CTGCAGCCAT CAGTCGTCAA GATAATCGGC TGCAGCAGTA GCAATAACAA TACCAGCCGC 
CAAGTGCCAT CGGGTGAGAG TGAGGACGAT CTCGTACAGA CAACGACCGG CCCTGCAACC 
CCTACCACCA CATTGTATGA TAACGAAAAT GAGTCATCCG ATCGGGAAGT GTCGCCCAAG 
TTTGTAGTCG GATCGAAAAA GTACGGTCGT CGGTCACGTC CCCGCGAGGA CTGCAACGGA 
CTGGACACTT CATCGGATTT CAGCAACTCG GACAACGAAT CGGTCCCCCA ACAACAACGT 
CAGCTTCAGC AAACGGTTCC ACCCTCCTCA GCGGAAGCAG ACAACTCCTC AAGTAAAAAC 
TCCTCCTCCA AAGTGCAACG ATCCGCCTCG CAGAGTGACA TTCATGGCTC AAAGCGACGG 
CGGCCTATGA GCGGAAGCAG GCTTAAGCGT TGCGCATCGC TACCTACGCA TCGACAGCGA 
CCGGATCTAG GCAAACTGAA CTCGAAGAAC GTACGCGATA AGCTCAACAT GGATGATAAG 
ATCATCGACA AGAAGATCAA CTTCATGAAA CTTTCGGAGA ATTTGCGGGA CATTTGGGAT 
ACTCTAGTGG GAAGCGGCCA CGGAGATCTA CTTAATCAAT CACAGCTGGA GGTCGTCTGT 
GAACGAGTAG GTCTACAAAA ACTACCCGCC AAGCTGGCCG CTCGGGAAGT TTTCGCGAAA 
TTGTCTCTCC AATCGATCGA GGGAATCAGC TACGATGATT TTATTTCGCT GCTGCAAAGC 
GATTCGGATA TTCTTGCCAT TGGCGGCGGC GGCGGCGGCG AAAAGGACCC GACAGGTGGC 
GGTGATAATG GCGTTCAGCG ATTGCGATTG TTCGACGAGA GCGACAGCTT CAAGGTTCAG 
GAACTGGATC GCGACAGCGT GGAGGAAACG GTTGGCTTCA CCGTGCGAAT GCCAGCCGAG 
TGGACAACCG AGATCGGATC ACTCTCGACG GCCATCATCA TCGACATGTG GGAGTCGGCC 
GGAATCAGCG AGCCGCGAGC TTTGCTGCAG GATCTCGGCT TCTACGGGCT GGAGGTGCAG 
GTAACGGAGC TAATCGCAGC ACTGGAGGAC GAACAGCAAC GGCTCGGCGA TGGCAACGAC 
ACCTCTGTGG TGGTGAGGGC ATCGCTGGCG CTTCACAAGG CGGAAGTCAG TGCCCTGCGA 
CAGGCGTTCC TTCAGCTGAT GGAAGAAAAC AAGAAACTCT ACTCCGACAA CAAAGAAGCC 
AATCAACGCG CCCAACTGCT GCTTCAGGAG GTGGACGAGC GGCACAACTC GTTGGAAAGT 
GCCACCCGGG ACAAAATCCG TCTCCTGGAG CAGCGTCACC ATGAAACGGT GAAAGAGCTG 
ACGTCGCAAC TGGCGTTTGA GAGGGAGCAA TTGTCCTGTG CGAATGCCAC CTTGGAGAAA 
CGGATCCAAA GCCTGGAACA GGACGAGAGC AAGGTGAAAG CGGAGATGAA CTCGCTGCTG 
GAGGAGAACG AGGGTTTGAG GGCTGAGCAG GAGAACCTCA CCAAGGAGCT GACGGATATT 
CTAGAGAAGA ATATCAAGCT CAATAAAGAT ATTGCCGAGC TGGAGGAAAA CAATCGCAAC 
GACTTCGATG ATGAATATGC CGGGAAGAAC GACCTGGAAG TTTTGGAGTT GATCGAGAAG 
AATTCTCTGT TGCAAGATAA AAATAAAGAA CTGAAAGATA AGAATGATGA GTTGAGTGTG 
GAGATTGAAA TGTTGAATGC CGAGCTGGCG AAGTTCAAAT TGAAGCGAAG TGGTGTTAGC 
AGGACAAGCT CGTTGGATGT GTCGCCGGTG ATGGTCGAAT CGACTGGGTG CGAAGAGAAT 
ACAAGTGCTG CTAGAAAGCG TCAAGGAGAT TCCCCAAGCA AGGCGAGACT CTCAGATGAA 
AGTCCCCGGT TGGGAAAATT CAGGAAGTGC AGCAACGATG TGGAGAATGA GTCCGATTCG 
AGTGGGGATT GGATGGCTCT GCATTCCGAG CTTGCACTGA AAGTGGAAGC TACCTCAGGT 
ATTAGTGTAG AAAAAACACA GGCAGAAGAA ATACAGCAAC TAAAGGCGAA AGTTGCTGAA 
CTAGAGGAGT TGAATAAGAA GAAAGAGGAA GAGAGTACTG GTTTTGAAAA GAGTGATTTG 
GAGAAAGTGT CCGATGGTAG TGATACAACA CAGTCCGAAA TGGCGAAAGT GAAGGCACGT 
TGCGAGGAGT TGGAAGCAAG TTTAGAACAA TTGAGCAAAG AATACGAAGC TTGTGAAGAC 
TACTGGCAGA GTAAGGTGAA CGAAGAAAGA TTGCTGTATG AAGAGGAACA GCGAATTGCC 
GACGAAAAAC TGAACGAGCT GTGTAAAAAG GTGGCCGAAT ACGAGGAGCA GTTTGCCGGC 
CAAGGTGGTA ATAAAAATGA TGATGGCCGA CTATCACCTA TCGAGGAGAA AGATGTCCTG 
GAGCAGCAGT ATCTGGATTT GGAGGTGGAA TTCCAACAGG TGCAGACTAT GCTGCAAGAA 
AAGACGCATG AACTGGAAAA GCTGCGGGGC AGGATCCTGG AGCTAGAACA ACTCGTCAAA 
TATCCATCGG AGGCGTTATT AGCTACGCCA CCACGTGTTG ATACGCCTGA AATGGAGAGC 
CCCGCCAGTT CACCAATCAG CTATCTATGG AATCAGAGCA CCATCCAAGC TCCAGCCACA 
CGGGACTACC AGAATCCGAA CTGGTGCAAG CCACTGAAAG CGAGCAGTAT TCCGCTTGAA 
ACGAATCTTG TTAATAACGA TGTGAACGAA TCGTTACCTC AATCCAAAAT GGCTGAAGAA 
CTGAAAGGCG ATGCCGACGA CTCCGCATTC GATCGTACGA TATCCCCAAT TCAGAAACCT 
ATCTCGAAAG AACTTATGGC CACATCTACG CCTTGTGTTG CAACAGCAGC AGCAGCTGAA 
AGTCAAGAAC TGGTGGATTG TTCCGACGTG TGCTCGGTAA AATCAGCTCG AAGCACTCAC 
AGTTTAGCCT CCACGCATAG CATTCACAAA TCCGTTTCAC AAACGGACGT CGCCTCGATC 
AACCAGGAGC AGTTGAAACT GCTGGAGCTG CAGGACGAGA TCAAAGAGCT GACCCACCAG 
CGAGACTGTC TGAAGATGGA GTTCCAGCAG TTGAACGAAG TGAAGCCGAT ATTGGCGCGG 
AGCTACTCCA AAACGGCGGC TCATCCAAAC CTCACGCAGA AGGTTCAGCA CCTGGAGCAG 
AAGAACCGGC AGCTGCATGC GATGATGAAA CAACAGCAGC ACCACTCGGA GCAGATTCTG 
GAACGAATCT GGATCCAACA GCGCAGCGAG ATCGCCGAGC TGCGCAATCA ACTCGACGCC 
CAGAACATGA TAATCGCCGA CCAAGCGTCC CGACTGGCCA ACAACGACCA GCTGGTGCGG 
GAACTGTACG TGGAAAATGG CCGACTAGTG GCGCAGGTCC AACGCCTGGA ACAGCAAAAG 
GCCCGGGCTA CCATGCTGCA GCTTTCGCAG GCCACTTCCG CCGGTGGCCA CTGCCATGCG 
GGCCAGCTGA GCGGATTCGT ACCTGGGATG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579519 ( XM_021848240.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021703932.1 Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAe124562
Clone ID Related Accession (Same CDS sequence)	XM_021848239.1 , XM_021848238.1 , XM_021848240.1
Accession Version	XM_021848240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	blastoderm-specific protein 25D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTTT CGACGGATCC GTACGAGCAG AAGCTGTACA GCTTGTTCCA GGAACACGAT 
GTCCATTCGC GGGGAAAGCT GGGCCGGGAA GCGCTGTTGA AGCTGTGCAA GACGTTGGAG 
TTGGCGAAAC CGACCGAGTT GCTGAAACGG CTGCTACGTG ACGGCAGCAC CAGTCAGGTT 
TCGTTCGGGG AATTCCGTGA AGGATTGCTG TTCATACTGA GCGGCGAGGA GCCGGAGGAA 
TCGATGTCAT CGTCGACGGC AACCGGGGCA CCGGGCCAGC AGCAACAGCA GCATGACCCG 
CCACGAACGG TGCCGGGTCA GAAAGATTCA TCCGAGGGAC AGAATGATGT GGCTACAGGT 
TTACCTACAG TTGCATCTCC GAGTGCAGTC TCCAACACCA GCAGAGTCGG GTCGCCATCG 
CAAGAGACGA CGACACCCTC GGGTAGCATA ATGTCCAACA CATCGAAACG TCCTCACAAA 
TTTACCTACC GCCGAGAAAA CGACCAGTCA ATTGCGGGCG AGAGCGAAGA CATCACATCT 
CTGCAGCCAT CAGTCGTCAA GATAATCGGC TGCAGCAGTA GCAATAACAA TACCAGCCGC 
CAAGTGCCAT CGGGTGAGAG TGAGGACGAT CTCGTACAGA CAACGACCGG CCCTGCAACC 
CCTACCACCA CATTGTATGA TAACGAAAAT GAGTCATCCG ATCGGGAAGT GTCGCCCAAG 
TTTGTAGTCG GATCGAAAAA GTACGGTCGT CGGTCACGTC CCCGCGAGGA CTGCAACGGA 
CTGGACACTT CATCGGATTT CAGCAACTCG GACAACGAAT CGGTCCCCCA ACAACAACGT 
CAGCTTCAGC AAACGGTTCC ACCCTCCTCA GCGGAAGCAG ACAACTCCTC AAGTAAAAAC 
TCCTCCTCCA AAGTGCAACG ATCCGCCTCG CAGAGTGACA TTCATGGCTC AAAGCGACGG 
CGGCCTATGA GCGGAAGCAG GCTTAAGCGT TGCGCATCGC TACCTACGCA TCGACAGCGA 
CCGGATCTAG GCAAACTGAA CTCGAAGAAC GTACGCGATA AGCTCAACAT GGATGATAAG 
ATCATCGACA AGAAGATCAA CTTCATGAAA CTTTCGGAGA ATTTGCGGGA CATTTGGGAT 
ACTCTAGTGG GAAGCGGCCA CGGAGATCTA CTTAATCAAT CACAGCTGGA GGTCGTCTGT 
GAACGAGTAG GTCTACAAAA ACTACCCGCC AAGCTGGCCG CTCGGGAAGT TTTCGCGAAA 
TTGTCTCTCC AATCGATCGA GGGAATCAGC TACGATGATT TTATTTCGCT GCTGCAAAGC 
GATTCGGATA TTCTTGCCAT TGGCGGCGGC GGCGGCGGCG AAAAGGACCC GACAGGTGGC 
GGTGATAATG GCGTTCAGCG ATTGCGATTG TTCGACGAGA GCGACAGCTT CAAGGTTCAG 
GAACTGGATC GCGACAGCGT GGAGGAAACG GTTGGCTTCA CCGTGCGAAT GCCAGCCGAG 
TGGACAACCG AGATCGGATC ACTCTCGACG GCCATCATCA TCGACATGTG GGAGTCGGCC 
GGAATCAGCG AGCCGCGAGC TTTGCTGCAG GATCTCGGCT TCTACGGGCT GGAGGTGCAG 
GTAACGGAGC TAATCGCAGC ACTGGAGGAC GAACAGCAAC GGCTCGGCGA TGGCAACGAC 
ACCTCTGTGG TGGTGAGGGC ATCGCTGGCG CTTCACAAGG CGGAAGTCAG TGCCCTGCGA 
CAGGCGTTCC TTCAGCTGAT GGAAGAAAAC AAGAAACTCT ACTCCGACAA CAAAGAAGCC 
AATCAACGCG CCCAACTGCT GCTTCAGGAG GTGGACGAGC GGCACAACTC GTTGGAAAGT 
GCCACCCGGG ACAAAATCCG TCTCCTGGAG CAGCGTCACC ATGAAACGGT GAAAGAGCTG 
ACGTCGCAAC TGGCGTTTGA GAGGGAGCAA TTGTCCTGTG CGAATGCCAC CTTGGAGAAA 
CGGATCCAAA GCCTGGAACA GGACGAGAGC AAGGTGAAAG CGGAGATGAA CTCGCTGCTG 
GAGGAGAACG AGGGTTTGAG GGCTGAGCAG GAGAACCTCA CCAAGGAGCT GACGGATATT 
CTAGAGAAGA ATATCAAGCT CAATAAAGAT ATTGCCGAGC TGGAGGAAAA CAATCGCAAC 
GACTTCGATG ATGAATATGC CGGGAAGAAC GACCTGGAAG TTTTGGAGTT GATCGAGAAG 
AATTCTCTGT TGCAAGATAA AAATAAAGAA CTGAAAGATA AGAATGATGA GTTGAGTGTG 
GAGATTGAAA TGTTGAATGC CGAGCTGGCG AAGTTCAAAT TGAAGCGAAG TGGTGTTAGC 
AGGACAAGCT CGTTGGATGT GTCGCCGGTG ATGGTCGAAT CGACTGGGTG CGAAGAGAAT 
ACAAGTGCTG CTAGAAAGCG TCAAGGAGAT TCCCCAAGCA AGGCGAGACT CTCAGATGAA 
AGTCCCCGGT TGGGAAAATT CAGGAAGTGC AGCAACGATG TGGAGAATGA GTCCGATTCG 
AGTGGGGATT GGATGGCTCT GCATTCCGAG CTTGCACTGA AAGTGGAAGC TACCTCAGGT 
ATTAGTGTAG AAAAAACACA GGCAGAAGAA ATACAGCAAC TAAAGGCGAA AGTTGCTGAA 
CTAGAGGAGT TGAATAAGAA GAAAGAGGAA GAGAGTACTG GTTTTGAAAA GAGTGATTTG 
GAGAAAGTGT CCGATGGTAG TGATACAACA CAGTCCGAAA TGGCGAAAGT GAAGGCACGT 
TGCGAGGAGT TGGAAGCAAG TTTAGAACAA TTGAGCAAAG AATACGAAGC TTGTGAAGAC 
TACTGGCAGA GTAAGGTGAA CGAAGAAAGA TTGCTGTATG AAGAGGAACA GCGAATTGCC 
GACGAAAAAC TGAACGAGCT GTGTAAAAAG GTGGCCGAAT ACGAGGAGCA GTTTGCCGGC 
CAAGGTGGTA ATAAAAATGA TGATGGCCGA CTATCACCTA TCGAGGAGAA AGATGTCCTG 
GAGCAGCAGT ATCTGGATTT GGAGGTGGAA TTCCAACAGG TGCAGACTAT GCTGCAAGAA 
AAGACGCATG AACTGGAAAA GCTGCGGGGC AGGATCCTGG AGCTAGAACA ACTCGTCAAA 
TATCCATCGG AGGCGTTATT AGCTACGCCA CCACGTGTTG ATACGCCTGA AATGGAGAGC 
CCCGCCAGTT CACCAATCAG CTATCTATGG AATCAGAGCA CCATCCAAGC TCCAGCCACA 
CGGGACTACC AGAATCCGAA CTGGTGCAAG CCACTGAAAG CGAGCAGTAT TCCGCTTGAA 
ACGAATCTTG TTAATAACGA TGTGAACGAA TCGTTACCTC AATCCAAAAT GGCTGAAGAA 
CTGAAAGGCG ATGCCGACGA CTCCGCATTC GATCGTACGA TATCCCCAAT TCAGAAACCT 
ATCTCGAAAG AACTTATGGC CACATCTACG CCTTGTGTTG CAACAGCAGC AGCAGCTGAA 
AGTCAAGAAC TGGTGGATTG TTCCGACGTG TGCTCGGTAA AATCAGCTCG AAGCACTCAC 
AGTTTAGCCT CCACGCATAG CATTCACAAA TCCGTTTCAC AAACGGACGT CGCCTCGATC 
AACCAGGAGC AGTTGAAACT GCTGGAGCTG CAGGACGAGA TCAAAGAGCT GACCCACCAG 
CGAGACTGTC TGAAGATGGA GTTCCAGCAG TTGAACGAAG TGAAGCCGAT ATTGGCGCGG 
AGCTACTCCA AAACGGCGGC TCATCCAAAC CTCACGCAGA AGGTTCAGCA CCTGGAGCAG 
AAGAACCGGC AGCTGCATGC GATGATGAAA CAACAGCAGC ACCACTCGGA GCAGATTCTG 
GAACGAATCT GGATCCAACA GCGCAGCGAG ATCGCCGAGC TGCGCAATCA ACTCGACGCC 
CAGAACATGA TAATCGCCGA CCAAGCGTCC CGACTGGCCA ACAACGACCA GCTGGTGCGG 
GAACTGTACG TGGAAAATGG CCGACTAGTG GCGCAGGTCC AACGCCTGGA ACAGCAAAAG 
GCCCGGGCTA CCATGCTGCA GCTTTCGCAG GCCACTTCCG CCGGTGGCCA CTGCCATGCG 
GGCCAGCTGA GCGGATTCGT ACCTGGGATG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021703932.1
CDS	552..4667
Translation	MALSTDPYEQKLYSLFQEHDVHSRGKLGREALLKLCKTLELAKPTELLKRLLRDGSTSQVSFGEFREGLLFILSGEEPEESMSSSTATGAPGQQQQQHDPPRTVPGQKDSSEGQNDVATGLPTVASPSAVSNTSRVGSPSQETTTPSGSIMSNTSKRPHKFTYRRENDQSIAGESEDITSLQPSVVKIIGCSSSNNNTSRQVPSGESEDDLVQTTTGPATPTTTLYDNENESSDREVSPKFVVGSKKYGRRSRPREDCNGLDTSSDFSNSDNESVPQQQRQLQQTVPPSSAEADNSSSKNSSSKVQRSASQSDIHGSKRRRPMSGSRLKRCASLPTHRQRPDLGKLNSKNVRDKLNMDDKIIDKKINFMKLSENLRDIWDTLVGSGHGDLLNQSQLEVVCERVGLQKLPAKLAAREVFAKLSLQSIEGISYDDFISLLQSDSDILAIGGGGGGEKDPTGGGDNGVQRLRLFDESDSFKVQELDRDSVEETVGFTVRMPAEWTTEIGSLSTAIIIDMWESAGISEPRALLQDLGFYGLEVQVTELIAALEDEQQRLGDGNDTSVVVRASLALHKAEVSALRQAFLQLMEENKKLYSDNKEANQRAQLLLQEVDERHNSLESATRDKIRLLEQRHHETVKELTSQLAFEREQLSCANATLEKRIQSLEQDESKVKAEMNSLLEENEGLRAEQENLTKELTDILEKNIKLNKDIAELEENNRNDFDDEYAGKNDLEVLELIEKNSLLQDKNKELKDKNDELSVEIEMLNAELAKFKLKRSGVSRTSSLDVSPVMVESTGCEENTSAARKRQGDSPSKARLSDESPRLGKFRKCSNDVENESDSSGDWMALHSELALKVEATSGISVEKTQAEEIQQLKAKVAELEELNKKKEEESTGFEKSDLEKVSDGSDTTQSEMAKVKARCEELEASLEQLSKEYEACEDYWQSKVNEERLLYEEEQRIADEKLNELCKKVAEYEEQFAGQGGNKNDDGRLSPIEEKDVLEQQYLDLEVEFQQVQTMLQEKTHELEKLRGRILELEQLVKYPSEALLATPPRVDTPEMESPASSPISYLWNQSTIQAPATRDYQNPNWCKPLKASSIPLETNLVNNDVNESLPQSKMAEELKGDADDSAFDRTISPIQKPISKELMATSTPCVATAAAAESQELVDCSDVCSVKSARSTHSLASTHSIHKSVSQTDVASINQEQLKLLELQDEIKELTHQRDCLKMEFQQLNEVKPILARSYSKTAAHPNLTQKVQHLEQKNRQLHAMMKQQQHHSEQILERIWIQQRSEIAELRNQLDAQNMIIADQASRLANNDQLVRELYVENGRLVAQVQRLEQQKARATMLQLSQATSAGGHCHAGQLSGFVPGMP

Target ORF information:

RefSeq Version	XM_021848240.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848240.1

ORF Insert Sequence:

ATGGCCCTTT CGACGGATCC GTACGAGCAG AAGCTGTACA GCTTGTTCCA GGAACACGAT 
GTCCATTCGC GGGGAAAGCT GGGCCGGGAA GCGCTGTTGA AGCTGTGCAA GACGTTGGAG 
TTGGCGAAAC CGACCGAGTT GCTGAAACGG CTGCTACGTG ACGGCAGCAC CAGTCAGGTT 
TCGTTCGGGG AATTCCGTGA AGGATTGCTG TTCATACTGA GCGGCGAGGA GCCGGAGGAA 
TCGATGTCAT CGTCGACGGC AACCGGGGCA CCGGGCCAGC AGCAACAGCA GCATGACCCG 
CCACGAACGG TGCCGGGTCA GAAAGATTCA TCCGAGGGAC AGAATGATGT GGCTACAGGT 
TTACCTACAG TTGCATCTCC GAGTGCAGTC TCCAACACCA GCAGAGTCGG GTCGCCATCG 
CAAGAGACGA CGACACCCTC GGGTAGCATA ATGTCCAACA CATCGAAACG TCCTCACAAA 
TTTACCTACC GCCGAGAAAA CGACCAGTCA ATTGCGGGCG AGAGCGAAGA CATCACATCT 
CTGCAGCCAT CAGTCGTCAA GATAATCGGC TGCAGCAGTA GCAATAACAA TACCAGCCGC 
CAAGTGCCAT CGGGTGAGAG TGAGGACGAT CTCGTACAGA CAACGACCGG CCCTGCAACC 
CCTACCACCA CATTGTATGA TAACGAAAAT GAGTCATCCG ATCGGGAAGT GTCGCCCAAG 
TTTGTAGTCG GATCGAAAAA GTACGGTCGT CGGTCACGTC CCCGCGAGGA CTGCAACGGA 
CTGGACACTT CATCGGATTT CAGCAACTCG GACAACGAAT CGGTCCCCCA ACAACAACGT 
CAGCTTCAGC AAACGGTTCC ACCCTCCTCA GCGGAAGCAG ACAACTCCTC AAGTAAAAAC 
TCCTCCTCCA AAGTGCAACG ATCCGCCTCG CAGAGTGACA TTCATGGCTC AAAGCGACGG 
CGGCCTATGA GCGGAAGCAG GCTTAAGCGT TGCGCATCGC TACCTACGCA TCGACAGCGA 
CCGGATCTAG GCAAACTGAA CTCGAAGAAC GTACGCGATA AGCTCAACAT GGATGATAAG 
ATCATCGACA AGAAGATCAA CTTCATGAAA CTTTCGGAGA ATTTGCGGGA CATTTGGGAT 
ACTCTAGTGG GAAGCGGCCA CGGAGATCTA CTTAATCAAT CACAGCTGGA GGTCGTCTGT 
GAACGAGTAG GTCTACAAAA ACTACCCGCC AAGCTGGCCG CTCGGGAAGT TTTCGCGAAA 
TTGTCTCTCC AATCGATCGA GGGAATCAGC TACGATGATT TTATTTCGCT GCTGCAAAGC 
GATTCGGATA TTCTTGCCAT TGGCGGCGGC GGCGGCGGCG AAAAGGACCC GACAGGTGGC 
GGTGATAATG GCGTTCAGCG ATTGCGATTG TTCGACGAGA GCGACAGCTT CAAGGTTCAG 
GAACTGGATC GCGACAGCGT GGAGGAAACG GTTGGCTTCA CCGTGCGAAT GCCAGCCGAG 
TGGACAACCG AGATCGGATC ACTCTCGACG GCCATCATCA TCGACATGTG GGAGTCGGCC 
GGAATCAGCG AGCCGCGAGC TTTGCTGCAG GATCTCGGCT TCTACGGGCT GGAGGTGCAG 
GTAACGGAGC TAATCGCAGC ACTGGAGGAC GAACAGCAAC GGCTCGGCGA TGGCAACGAC 
ACCTCTGTGG TGGTGAGGGC ATCGCTGGCG CTTCACAAGG CGGAAGTCAG TGCCCTGCGA 
CAGGCGTTCC TTCAGCTGAT GGAAGAAAAC AAGAAACTCT ACTCCGACAA CAAAGAAGCC 
AATCAACGCG CCCAACTGCT GCTTCAGGAG GTGGACGAGC GGCACAACTC GTTGGAAAGT 
GCCACCCGGG ACAAAATCCG TCTCCTGGAG CAGCGTCACC ATGAAACGGT GAAAGAGCTG 
ACGTCGCAAC TGGCGTTTGA GAGGGAGCAA TTGTCCTGTG CGAATGCCAC CTTGGAGAAA 
CGGATCCAAA GCCTGGAACA GGACGAGAGC AAGGTGAAAG CGGAGATGAA CTCGCTGCTG 
GAGGAGAACG AGGGTTTGAG GGCTGAGCAG GAGAACCTCA CCAAGGAGCT GACGGATATT 
CTAGAGAAGA ATATCAAGCT CAATAAAGAT ATTGCCGAGC TGGAGGAAAA CAATCGCAAC 
GACTTCGATG ATGAATATGC CGGGAAGAAC GACCTGGAAG TTTTGGAGTT GATCGAGAAG 
AATTCTCTGT TGCAAGATAA AAATAAAGAA CTGAAAGATA AGAATGATGA GTTGAGTGTG 
GAGATTGAAA TGTTGAATGC CGAGCTGGCG AAGTTCAAAT TGAAGCGAAG TGGTGTTAGC 
AGGACAAGCT CGTTGGATGT GTCGCCGGTG ATGGTCGAAT CGACTGGGTG CGAAGAGAAT 
ACAAGTGCTG CTAGAAAGCG TCAAGGAGAT TCCCCAAGCA AGGCGAGACT CTCAGATGAA 
AGTCCCCGGT TGGGAAAATT CAGGAAGTGC AGCAACGATG TGGAGAATGA GTCCGATTCG 
AGTGGGGATT GGATGGCTCT GCATTCCGAG CTTGCACTGA AAGTGGAAGC TACCTCAGGT 
ATTAGTGTAG AAAAAACACA GGCAGAAGAA ATACAGCAAC TAAAGGCGAA AGTTGCTGAA 
CTAGAGGAGT TGAATAAGAA GAAAGAGGAA GAGAGTACTG GTTTTGAAAA GAGTGATTTG 
GAGAAAGTGT CCGATGGTAG TGATACAACA CAGTCCGAAA TGGCGAAAGT GAAGGCACGT 
TGCGAGGAGT TGGAAGCAAG TTTAGAACAA TTGAGCAAAG AATACGAAGC TTGTGAAGAC 
TACTGGCAGA GTAAGGTGAA CGAAGAAAGA TTGCTGTATG AAGAGGAACA GCGAATTGCC 
GACGAAAAAC TGAACGAGCT GTGTAAAAAG GTGGCCGAAT ACGAGGAGCA GTTTGCCGGC 
CAAGGTGGTA ATAAAAATGA TGATGGCCGA CTATCACCTA TCGAGGAGAA AGATGTCCTG 
GAGCAGCAGT ATCTGGATTT GGAGGTGGAA TTCCAACAGG TGCAGACTAT GCTGCAAGAA 
AAGACGCATG AACTGGAAAA GCTGCGGGGC AGGATCCTGG AGCTAGAACA ACTCGTCAAA 
TATCCATCGG AGGCGTTATT AGCTACGCCA CCACGTGTTG ATACGCCTGA AATGGAGAGC 
CCCGCCAGTT CACCAATCAG CTATCTATGG AATCAGAGCA CCATCCAAGC TCCAGCCACA 
CGGGACTACC AGAATCCGAA CTGGTGCAAG CCACTGAAAG CGAGCAGTAT TCCGCTTGAA 
ACGAATCTTG TTAATAACGA TGTGAACGAA TCGTTACCTC AATCCAAAAT GGCTGAAGAA 
CTGAAAGGCG ATGCCGACGA CTCCGCATTC GATCGTACGA TATCCCCAAT TCAGAAACCT 
ATCTCGAAAG AACTTATGGC CACATCTACG CCTTGTGTTG CAACAGCAGC AGCAGCTGAA 
AGTCAAGAAC TGGTGGATTG TTCCGACGTG TGCTCGGTAA AATCAGCTCG AAGCACTCAC 
AGTTTAGCCT CCACGCATAG CATTCACAAA TCCGTTTCAC AAACGGACGT CGCCTCGATC 
AACCAGGAGC AGTTGAAACT GCTGGAGCTG CAGGACGAGA TCAAAGAGCT GACCCACCAG 
CGAGACTGTC TGAAGATGGA GTTCCAGCAG TTGAACGAAG TGAAGCCGAT ATTGGCGCGG 
AGCTACTCCA AAACGGCGGC TCATCCAAAC CTCACGCAGA AGGTTCAGCA CCTGGAGCAG 
AAGAACCGGC AGCTGCATGC GATGATGAAA CAACAGCAGC ACCACTCGGA GCAGATTCTG 
GAACGAATCT GGATCCAACA GCGCAGCGAG ATCGCCGAGC TGCGCAATCA ACTCGACGCC 
CAGAACATGA TAATCGCCGA CCAAGCGTCC CGACTGGCCA ACAACGACCA GCTGGTGCGG 
GAACTGTACG TGGAAAATGG CCGACTAGTG GCGCAGGTCC AACGCCTGGA ACAGCAAAAG 
GCCCGGGCTA CCATGCTGCA GCTTTCGCAG GCCACTTCCG CCGGTGGCCA CTGCCATGCG 
GGCCAGCTGA GCGGATTCGT ACCTGGGATG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5579519 ( XM_021848241.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021703933.1 Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X4, mRNA.

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CloneID	OAe124563
Clone ID Related Accession (Same CDS sequence)	XM_021848241.1
Accession Version	XM_021848241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	blastoderm-specific protein 25D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAATCA TTCTTTATTC ATCCGAGGGA CAGAATGATG TGGCTACAGG TTTACCTACA 
GTTGCATCTC CGAGTGCAGT CTCCAACACC AGCAGAGTCG GGTCGCCATC GCAAGAGACG 
ACGACACCCT CGGGTAGCAT AATGTCCAAC ACATCGAAAC GTCCTCACAA ATTTACCTAC 
CGCCGAGAAA ACGACCAGTC AATTGCGGGC GAGAGCGAAG ACATCACATC TCTGCAGCCA 
TCAGTCGTCA AGATAATCGG CTGCAGCAGT AGCAATAACA ATACCAGCCG CCAAGTGCCA 
TCGGGTGAGA GTGAGGACGA TCTCGTACAG ACAACGACCG GCCCTGCAAC CCCTACCACC 
ACATTGTATG ATAACGAAAA TGAGTCATCC GATCGGGAAG TGTCGCCCAA GTTTGTAGTC 
GGATCGAAAA AGTACGGTCG TCGGTCACGT CCCCGCGAGG ACTGCAACGG ACTGGACACT 
TCATCGGATT TCAGCAACTC GGACAACGAA TCGGTCCCCC AACAACAACG TCAGCTTCAG 
CAAACGGTTC CACCCTCCTC AGCGGAAGCA GACAACTCCT CAAGTAAAAA CTCCTCCTCC 
AAAGTGCAAC GATCCGCCTC GCAGAGTGAC ATTCATGGCT CAAAGCGACG GCGGCCTATG 
AGCGGAAGCA GGCTTAAGCG TTGCGCATCG CTACCTACGC ATCGACAGCG ACCGGATCTA 
GGCAAACTGA ACTCGAAGAA CGTACGCGAT AAGCTCAACA TGGATGATAA GATCATCGAC 
AAGAAGATCA ACTTCATGAA ACTTTCGGAG AATTTGCGGG ACATTTGGGA TACTCTAGTG 
GGAAGCGGCC ACGGAGATCT ACTTAATCAA TCACAGCTGG AGGTCGTCTG TGAACGAGTA 
GGTCTACAAA AACTACCCGC CAAGCTGGCC GCTCGGGAAG TTTTCGCGAA ATTGTCTCTC 
CAATCGATCG AGGGAATCAG CTACGATGAT TTTATTTCGC TGCTGCAAAG CGATTCGGAT 
ATTCTTGCCA TTGGCGGCGG CGGCGGCGGC GAAAAGGACC CGACAGGTGG CGGTGATAAT 
GGCGTTCAGC GATTGCGATT GTTCGACGAG AGCGACAGCT TCAAGGTTCA GGAACTGGAT 
CGCGACAGCG TGGAGGAAAC GGTTGGCTTC ACCGTGCGAA TGCCAGCCGA GTGGACAACC 
GAGATCGGAT CACTCTCGAC GGCCATCATC ATCGACATGT GGGAGTCGGC CGGAATCAGC 
GAGCCGCGAG CTTTGCTGCA GGATCTCGGC TTCTACGGGC TGGAGGTGCA GGTAACGGAG 
CTAATCGCAG CACTGGAGGA CGAACAGCAA CGGCTCGGCG ATGGCAACGA CACCTCTGTG 
GTGGTGAGGG CATCGCTGGC GCTTCACAAG GCGGAAGTCA GTGCCCTGCG ACAGGCGTTC 
CTTCAGCTGA TGGAAGAAAA CAAGAAACTC TACTCCGACA ACAAAGAAGC CAATCAACGC 
GCCCAACTGC TGCTTCAGGA GGTGGACGAG CGGCACAACT CGTTGGAAAG TGCCACCCGG 
GACAAAATCC GTCTCCTGGA GCAGCGTCAC CATGAAACGG TGAAAGAGCT GACGTCGCAA 
CTGGCGTTTG AGAGGGAGCA ATTGTCCTGT GCGAATGCCA CCTTGGAGAA ACGGATCCAA 
AGCCTGGAAC AGGACGAGAG CAAGGTGAAA GCGGAGATGA ACTCGCTGCT GGAGGAGAAC 
GAGGGTTTGA GGGCTGAGCA GGAGAACCTC ACCAAGGAGC TGACGGATAT TCTAGAGAAG 
AATATCAAGC TCAATAAAGA TATTGCCGAG CTGGAGGAAA ACAATCGCAA CGACTTCGAT 
GATGAATATG CCGGGAAGAA CGACCTGGAA GTTTTGGAGT TGATCGAGAA GAATTCTCTG 
TTGCAAGATA AAAATAAAGA ACTGAAAGAT AAGAATGATG AGTTGAGTGT GGAGATTGAA 
ATGTTGAATG CCGAGCTGGC GAAGTTCAAA TTGAAGCGAA GTGGTGTTAG CAGGACAAGC 
TCGTTGGATG TGTCGCCGGT GATGGTCGAA TCGACTGGGT GCGAAGAGAA TACAAGTGCT 
GCTAGAAAGC GTCAAGGAGA TTCCCCAAGC AAGGCGAGAC TCTCAGATGA AAGTCCCCGG 
TTGGGAAAAT TCAGGAAGTG CAGCAACGAT GTGGAGAATG AGTCCGATTC GAGTGGGGAT 
TGGATGGCTC TGCATTCCGA GCTTGCACTG AAAGTGGAAG CTACCTCAGG TATTAGTGTA 
GAAAAAACAC AGGCAGAAGA AATACAGCAA CTAAAGGCGA AAGTTGCTGA ACTAGAGGAG 
TTGAATAAGA AGAAAGAGGA AGAGAGTACT GGTTTTGAAA AGAGTGATTT GGAGAAAGTG 
TCCGATGGTA GTGATACAAC ACAGTCCGAA ATGGCGAAAG TGAAGGCACG TTGCGAGGAG 
TTGGAAGCAA GTTTAGAACA ATTGAGCAAA GAATACGAAG CTTGTGAAGA CTACTGGCAG 
AGTAAGGTGA ACGAAGAAAG ATTGCTGTAT GAAGAGGAAC AGCGAATTGC CGACGAAAAA 
CTGAACGAGC TGTGTAAAAA GGTGGCCGAA TACGAGGAGC AGTTTGCCGG CCAAGGTGGT 
AATAAAAATG ATGATGGCCG ACTATCACCT ATCGAGGAGA AAGATGTCCT GGAGCAGCAG 
TATCTGGATT TGGAGGTGGA ATTCCAACAG GTGCAGACTA TGCTGCAAGA AAAGACGCAT 
GAACTGGAAA AGCTGCGGGG CAGGATCCTG GAGCTAGAAC AACTCGTCAA ATATCCATCG 
GAGGCGTTAT TAGCTACGCC ACCACGTGTT GATACGCCTG AAATGGAGAG CCCCGCCAGT 
TCACCAATCA GCTATCTATG GAATCAGAGC ACCATCCAAG CTCCAGCCAC ACGGGACTAC 
CAGAATCCGA ACTGGTGCAA GCCACTGAAA GCGAGCAGTA TTCCGCTTGA AACGAATCTT 
GTTAATAACG ATGTGAACGA ATCGTTACCT CAATCCAAAA TGGCTGAAGA ACTGAAAGGC 
GATGCCGACG ACTCCGCATT CGATCGTACG ATATCCCCAA TTCAGAAACC TATCTCGAAA 
GAACTTATGG CCACATCTAC GCCTTGTGTT GCAACAGCAG CAGCAGCTGA AAGTCAAGAA 
CTGGTGGATT GTTCCGACGT GTGCTCGGTA AAATCAGCTC GAAGCACTCA CAGTTTAGCC 
TCCACGCATA GCATTCACAA ATCCGTTTCA CAAACGGACG TCGCCTCGAT CAACCAGGAG 
CAGTTGAAAC TGCTGGAGCT GCAGGACGAG ATCAAAGAGC TGACCCACCA GCGAGACTGT 
CTGAAGATGG AGTTCCAGCA GTTGAACGAA GTGAAGCCGA TATTGGCGCG GAGCTACTCC 
AAAACGGCGG CTCATCCAAA CCTCACGCAG AAGGTTCAGC ACCTGGAGCA GAAGAACCGG 
CAGCTGCATG CGATGATGAA ACAACAGCAG CACCACTCGG AGCAGATTCT GGAACGAATC 
TGGATCCAAC AGCGCAGCGA GATCGCCGAG CTGCGCAATC AACTCGACGC CCAGAACATG 
ATAATCGCCG ACCAAGCGTC CCGACTGGCC AACAACGACC AGCTGGTGCG GGAACTGTAC 
GTGGAAAATG GCCGACTAGT GGCGCAGGTC CAACGCCTGG AACAGCAAAA GGCCCGGGCT 
ACCATGCTGC AGCTTTCGCA GGCCACTTCC GCCGGTGGCC ACTGCCATGC GGGCCAGCTG 
AGCGGATTCG TACCTGGGAT GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021703933.1
CDS	243..4049
Translation	MIIILYSSEGQNDVATGLPTVASPSAVSNTSRVGSPSQETTTPSGSIMSNTSKRPHKFTYRRENDQSIAGESEDITSLQPSVVKIIGCSSSNNNTSRQVPSGESEDDLVQTTTGPATPTTTLYDNENESSDREVSPKFVVGSKKYGRRSRPREDCNGLDTSSDFSNSDNESVPQQQRQLQQTVPPSSAEADNSSSKNSSSKVQRSASQSDIHGSKRRRPMSGSRLKRCASLPTHRQRPDLGKLNSKNVRDKLNMDDKIIDKKINFMKLSENLRDIWDTLVGSGHGDLLNQSQLEVVCERVGLQKLPAKLAAREVFAKLSLQSIEGISYDDFISLLQSDSDILAIGGGGGGEKDPTGGGDNGVQRLRLFDESDSFKVQELDRDSVEETVGFTVRMPAEWTTEIGSLSTAIIIDMWESAGISEPRALLQDLGFYGLEVQVTELIAALEDEQQRLGDGNDTSVVVRASLALHKAEVSALRQAFLQLMEENKKLYSDNKEANQRAQLLLQEVDERHNSLESATRDKIRLLEQRHHETVKELTSQLAFEREQLSCANATLEKRIQSLEQDESKVKAEMNSLLEENEGLRAEQENLTKELTDILEKNIKLNKDIAELEENNRNDFDDEYAGKNDLEVLELIEKNSLLQDKNKELKDKNDELSVEIEMLNAELAKFKLKRSGVSRTSSLDVSPVMVESTGCEENTSAARKRQGDSPSKARLSDESPRLGKFRKCSNDVENESDSSGDWMALHSELALKVEATSGISVEKTQAEEIQQLKAKVAELEELNKKKEEESTGFEKSDLEKVSDGSDTTQSEMAKVKARCEELEASLEQLSKEYEACEDYWQSKVNEERLLYEEEQRIADEKLNELCKKVAEYEEQFAGQGGNKNDDGRLSPIEEKDVLEQQYLDLEVEFQQVQTMLQEKTHELEKLRGRILELEQLVKYPSEALLATPPRVDTPEMESPASSPISYLWNQSTIQAPATRDYQNPNWCKPLKASSIPLETNLVNNDVNESLPQSKMAEELKGDADDSAFDRTISPIQKPISKELMATSTPCVATAAAAESQELVDCSDVCSVKSARSTHSLASTHSIHKSVSQTDVASINQEQLKLLELQDEIKELTHQRDCLKMEFQQLNEVKPILARSYSKTAAHPNLTQKVQHLEQKNRQLHAMMKQQQHHSEQILERIWIQQRSEIAELRNQLDAQNMIIADQASRLANNDQLVRELYVENGRLVAQVQRLEQQKARATMLQLSQATSAGGHCHAGQLSGFVPGMP

Target ORF information:

RefSeq Version	XM_021848241.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti blastoderm-specific protein 25D (LOC5579519), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848241.1

ORF Insert Sequence:

ATGATAATCA TTCTTTATTC ATCCGAGGGA CAGAATGATG TGGCTACAGG TTTACCTACA 
GTTGCATCTC CGAGTGCAGT CTCCAACACC AGCAGAGTCG GGTCGCCATC GCAAGAGACG 
ACGACACCCT CGGGTAGCAT AATGTCCAAC ACATCGAAAC GTCCTCACAA ATTTACCTAC 
CGCCGAGAAA ACGACCAGTC AATTGCGGGC GAGAGCGAAG ACATCACATC TCTGCAGCCA 
TCAGTCGTCA AGATAATCGG CTGCAGCAGT AGCAATAACA ATACCAGCCG CCAAGTGCCA 
TCGGGTGAGA GTGAGGACGA TCTCGTACAG ACAACGACCG GCCCTGCAAC CCCTACCACC 
ACATTGTATG ATAACGAAAA TGAGTCATCC GATCGGGAAG TGTCGCCCAA GTTTGTAGTC 
GGATCGAAAA AGTACGGTCG TCGGTCACGT CCCCGCGAGG ACTGCAACGG ACTGGACACT 
TCATCGGATT TCAGCAACTC GGACAACGAA TCGGTCCCCC AACAACAACG TCAGCTTCAG 
CAAACGGTTC CACCCTCCTC AGCGGAAGCA GACAACTCCT CAAGTAAAAA CTCCTCCTCC 
AAAGTGCAAC GATCCGCCTC GCAGAGTGAC ATTCATGGCT CAAAGCGACG GCGGCCTATG 
AGCGGAAGCA GGCTTAAGCG TTGCGCATCG CTACCTACGC ATCGACAGCG ACCGGATCTA 
GGCAAACTGA ACTCGAAGAA CGTACGCGAT AAGCTCAACA TGGATGATAA GATCATCGAC 
AAGAAGATCA ACTTCATGAA ACTTTCGGAG AATTTGCGGG ACATTTGGGA TACTCTAGTG 
GGAAGCGGCC ACGGAGATCT ACTTAATCAA TCACAGCTGG AGGTCGTCTG TGAACGAGTA 
GGTCTACAAA AACTACCCGC CAAGCTGGCC GCTCGGGAAG TTTTCGCGAA ATTGTCTCTC 
CAATCGATCG AGGGAATCAG CTACGATGAT TTTATTTCGC TGCTGCAAAG CGATTCGGAT 
ATTCTTGCCA TTGGCGGCGG CGGCGGCGGC GAAAAGGACC CGACAGGTGG CGGTGATAAT 
GGCGTTCAGC GATTGCGATT GTTCGACGAG AGCGACAGCT TCAAGGTTCA GGAACTGGAT 
CGCGACAGCG TGGAGGAAAC GGTTGGCTTC ACCGTGCGAA TGCCAGCCGA GTGGACAACC 
GAGATCGGAT CACTCTCGAC GGCCATCATC ATCGACATGT GGGAGTCGGC CGGAATCAGC 
GAGCCGCGAG CTTTGCTGCA GGATCTCGGC TTCTACGGGC TGGAGGTGCA GGTAACGGAG 
CTAATCGCAG CACTGGAGGA CGAACAGCAA CGGCTCGGCG ATGGCAACGA CACCTCTGTG 
GTGGTGAGGG CATCGCTGGC GCTTCACAAG GCGGAAGTCA GTGCCCTGCG ACAGGCGTTC 
CTTCAGCTGA TGGAAGAAAA CAAGAAACTC TACTCCGACA ACAAAGAAGC CAATCAACGC 
GCCCAACTGC TGCTTCAGGA GGTGGACGAG CGGCACAACT CGTTGGAAAG TGCCACCCGG 
GACAAAATCC GTCTCCTGGA GCAGCGTCAC CATGAAACGG TGAAAGAGCT GACGTCGCAA 
CTGGCGTTTG AGAGGGAGCA ATTGTCCTGT GCGAATGCCA CCTTGGAGAA ACGGATCCAA 
AGCCTGGAAC AGGACGAGAG CAAGGTGAAA GCGGAGATGA ACTCGCTGCT GGAGGAGAAC 
GAGGGTTTGA GGGCTGAGCA GGAGAACCTC ACCAAGGAGC TGACGGATAT TCTAGAGAAG 
AATATCAAGC TCAATAAAGA TATTGCCGAG CTGGAGGAAA ACAATCGCAA CGACTTCGAT 
GATGAATATG CCGGGAAGAA CGACCTGGAA GTTTTGGAGT TGATCGAGAA GAATTCTCTG 
TTGCAAGATA AAAATAAAGA ACTGAAAGAT AAGAATGATG AGTTGAGTGT GGAGATTGAA 
ATGTTGAATG CCGAGCTGGC GAAGTTCAAA TTGAAGCGAA GTGGTGTTAG CAGGACAAGC 
TCGTTGGATG TGTCGCCGGT GATGGTCGAA TCGACTGGGT GCGAAGAGAA TACAAGTGCT 
GCTAGAAAGC GTCAAGGAGA TTCCCCAAGC AAGGCGAGAC TCTCAGATGA AAGTCCCCGG 
TTGGGAAAAT TCAGGAAGTG CAGCAACGAT GTGGAGAATG AGTCCGATTC GAGTGGGGAT 
TGGATGGCTC TGCATTCCGA GCTTGCACTG AAAGTGGAAG CTACCTCAGG TATTAGTGTA 
GAAAAAACAC AGGCAGAAGA AATACAGCAA CTAAAGGCGA AAGTTGCTGA ACTAGAGGAG 
TTGAATAAGA AGAAAGAGGA AGAGAGTACT GGTTTTGAAA AGAGTGATTT GGAGAAAGTG 
TCCGATGGTA GTGATACAAC ACAGTCCGAA ATGGCGAAAG TGAAGGCACG TTGCGAGGAG 
TTGGAAGCAA GTTTAGAACA ATTGAGCAAA GAATACGAAG CTTGTGAAGA CTACTGGCAG 
AGTAAGGTGA ACGAAGAAAG ATTGCTGTAT GAAGAGGAAC AGCGAATTGC CGACGAAAAA 
CTGAACGAGC TGTGTAAAAA GGTGGCCGAA TACGAGGAGC AGTTTGCCGG CCAAGGTGGT 
AATAAAAATG ATGATGGCCG ACTATCACCT ATCGAGGAGA AAGATGTCCT GGAGCAGCAG 
TATCTGGATT TGGAGGTGGA ATTCCAACAG GTGCAGACTA TGCTGCAAGA AAAGACGCAT 
GAACTGGAAA AGCTGCGGGG CAGGATCCTG GAGCTAGAAC AACTCGTCAA ATATCCATCG 
GAGGCGTTAT TAGCTACGCC ACCACGTGTT GATACGCCTG AAATGGAGAG CCCCGCCAGT 
TCACCAATCA GCTATCTATG GAATCAGAGC ACCATCCAAG CTCCAGCCAC ACGGGACTAC 
CAGAATCCGA ACTGGTGCAA GCCACTGAAA GCGAGCAGTA TTCCGCTTGA AACGAATCTT 
GTTAATAACG ATGTGAACGA ATCGTTACCT CAATCCAAAA TGGCTGAAGA ACTGAAAGGC 
GATGCCGACG ACTCCGCATT CGATCGTACG ATATCCCCAA TTCAGAAACC TATCTCGAAA 
GAACTTATGG CCACATCTAC GCCTTGTGTT GCAACAGCAG CAGCAGCTGA AAGTCAAGAA 
CTGGTGGATT GTTCCGACGT GTGCTCGGTA AAATCAGCTC GAAGCACTCA CAGTTTAGCC 
TCCACGCATA GCATTCACAA ATCCGTTTCA CAAACGGACG TCGCCTCGAT CAACCAGGAG 
CAGTTGAAAC TGCTGGAGCT GCAGGACGAG ATCAAAGAGC TGACCCACCA GCGAGACTGT 
CTGAAGATGG AGTTCCAGCA GTTGAACGAA GTGAAGCCGA TATTGGCGCG GAGCTACTCC 
AAAACGGCGG CTCATCCAAA CCTCACGCAG AAGGTTCAGC ACCTGGAGCA GAAGAACCGG 
CAGCTGCATG CGATGATGAA ACAACAGCAG CACCACTCGG AGCAGATTCT GGAACGAATC 
TGGATCCAAC AGCGCAGCGA GATCGCCGAG CTGCGCAATC AACTCGACGC CCAGAACATG 
ATAATCGCCG ACCAAGCGTC CCGACTGGCC AACAACGACC AGCTGGTGCG GGAACTGTAC 
GTGGAAAATG GCCGACTAGT GGCGCAGGTC CAACGCCTGG AACAGCAAAA GGCCCGGGCT 
ACCATGCTGC AGCTTTCGCA GGCCACTTCC GCCGGTGGCC ACTGCCATGC GGGCCAGCTG 
AGCGGATTCG TACCTGGGAT GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5579519 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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