AAEL_AAEL013108 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL013108
Entrez Gene ID	5577249
Full Name	WASH complex subunit 4
Synonyms	AAEL013108
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021849203.1	XP_021704895.1	WASH complex subunit 4
XM_001656301.1	XP_001656351.1	AAEL013108-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL013108 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL013108 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe12027	XM_001656301.1 Latest version!	Aedes aegypti AAEL013108-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAe132244	XM_021849203.1 Latest version!	Aedes aegypti WASH complex subunit 4 (LOC5577249), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL013108 ( XM_001656301.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001656351.1 Aedes aegypti AAEL013108-RA partial mRNA.

Price & Availability↑

CloneID	OAe12027
Clone ID Related Accession (Same CDS sequence)	XM_001656301.1
Accession Version	XM_001656301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL013108-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811564). COMPLETENESS: incomplete on the 5' end.

ATGTTGGAAG CCATGTTCAA TGCCAAGCAG AAGTTGGTTC TCGAGTCGGC AAACGTTTTG 
TCGGATAAGT TTATGAAATA TATCAGACAA CGTTTAGTAG AGGTTGGCAG TTACAATGCC 
AATCTGTCTA GCATTGACGA GACTACCAAC GTCGTTAAGC TCAATGCCAT GATTAACTTG 
TATCATTACA TATTCGTGAG CATTGACAAC AAGGTGGTTA AAAGTTTGAT GGATATCAAT 
GATAAGTTTT GTGGCGTGCC GCTTATCGGG AAAGTGATGT GGATTCCGGA CGACTTTTTC 
CGGAAATATG GCCACAAGTC GATTCGCGAT TGCGATCGCA ACGATTACCG CAAGGCACGT 
GAGGCTGCCC TTATCTACCG GAAGAAAAGC ATGGTCAAGG AAATCCTCGT ATATTGTATC 
CAGATAACGA GCTGGTTGGT TCGGACCACG GCTGCGTTCA AGACGACAAA ATACGACACC 
ACGTTGGACA CTTTGCGGCA AAAGTGCGGC CTAATCTTCG AGGGTATTCA CTATGCGCAG 
GAGATCAGCT TTCTGGTGCA ATCGATAACG TCGCTGCATT TGCTGATGCA CGAATCCATC 
AGCCAGCAAA TGTTTCATGC GGTGGGAAAA TTGCTAGAAT ATCTACAGGC CGTCCAGAAC 
TTTTTCGATA GCAATCAGCA AGCCATCGCA GAAACGAACC AGTTCATCAT TCAACATTTG 
CAGCATAAGA TCTTCGTTAT TTTAATAACC GATGCTAAAC AAGCCAAGCT AAAAAAGAGC 
GAAATTTTCA TCGATAAACT GTCGGCCTTC CACGTGATTG AACGCTGTAT GAGTGGACCG 
GCGAATCGCA CCCGTTTGAC CATCGCACGT TTGGCCATTT CCATAAGTGA TCCGCGTCAA 
ACTCTCTCTC CCGAGAGCTA CGAAAAAATC CGCATACTTT TGGATCAACT GGACATTCTG 
GCCAATCTGC AAACTAAGCT TCGAAACCTC AGCGATACAC AATTTCTGTA CTGGCACCAA 
ACGTTCTTTT CGCTGTACGT TTCCGATTTC TACGACCATA ATACAGCTCA GAATAACTTG 
AAATATTTGT TGCAATCAGC TGCTACCTTT GCCTCCGATA ATACCACGCA AAATGCCATA 
GCTGTTTTAT CGAAAAACCA TCGGAAATCA TTTCAAGATA AATTGCTACA TCAAATAAGC 
AATCACATTG AAACGTACAT CCGGCTAGAC TACTATGGGA AAACGTACCA GATTGAACCG 
TACAATCCTT TCACCGATAC CACAATGGCA CTGAGTAACA TCTACTCTCT GGTGAGGATG 
GAACCCATCC TTGTCGGAGG TCAGTACCTA AGCGTGAAGG AACACGTTGA ACGATATCTG 
AGCAACACGT ACTACACGTT GGCGTCGATT TCGCAACATG ATTGGAAGAT CTACAAGGAA 
ATGCGATGTC ACGCGGCCTG TTTCCAGATT GAAACCATCG AGGATCAGCT ACCTAAGCAA 
ACGTTAGAGC AGGGGCTGGA TGTGCTGGAG ATTATGCACA ACATCGAACA TTTTGTAAGC 
CACTACGTGT ACAATTTGAA CTTCCAGATA TTCATCGAAC AGTCGAGTAC CAACCACTTC 
CTGAACACGG TCAACATTGG ACACATTGCG AATTCGCTGC GGCGCCACGG CAACGGCATC 
ATTAACACTA CGGTCAACTA CACATACCAG TTTCTTCGGC AAAAGTTCTT CGCCTTTTCG 
GACTTTTTGT ACGACGAACA GATCAAAGCT CGTTTAACCA GCGACGCCAA ATATTTCCTA 
GAAAATGGTG AGGCGCTCAA TCAAACGTAT GATTACAATC GAGCCAATGC TTTCAATCGC 
AAAATAAAGA ACCTAGGCCT GTCGGAAGCA GGCGAAACCT ATATGGATCT GTTCAGGAAG 
TTGATTTGTC ACATTGGCAA TGCCATGGGA TACGTTCGGA TGATACGTTC CGGAGCGCTG 
CACGAATGTA CGGAAGCTAC CGTATACTTG CCCGTCATAG ACGGGGATCT CAATTTCTCA 
AAATTTTGCA AAGAAGAATC CTTGCACGAT TTGACGATCT CAGCGGCGGA GATCTTGGAA 
AATGATATCA ACAACTTGTG TAAAAACTAT CGAATTGATA CCAACTATTT CCGTTTGCTG 
GTGAATGCCT TTCTGTCTTT ACGGCACGCG GAAAATATTC ATCTGCAAAA CTTCTACATG 
ATTGTACCCC CTCTGACCAT CAACTTTGTG GAGTACATTT TGAAGGCGAA GGAGAAAATT 
ACCAAGAAGG ATAAAGTAGG TGCCTTATTT ACCGACGATG GATTTGCCAT GGGAATTGCT 
TACATCCTCA AACTGTTGGA CCAAACTACT AAGTTCAACT CCCTGCATTG GTTCCGTTCG 
GTGAAGAACA AATACAGCCA GGAAATCGAA AAACTAGATA CCCAACAGCA GCAATCGGAC 
AAAACGACCG ACGGCGACAA ATTGCAGCAA ACATTCGCTT TGACGCGAAA ACGGCTCAAC 
ATGGTCCAGC AGGAATTTGA TCTTCTGTTT TGTAATTTGA GCAGTGCAAA GATATTCTTC 
AACGACGCTG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001656351.1
CDS	1..2598
Translation	MLEAMFNAKQKLVLESANVLSDKFMKYIRQRLVEVGSYNANLSSIDETTNVVKLNAMINLYHYIFVSIDNKVVKSLMDINDKFCGVPLIGKVMWIPDDFFRKYGHKSIRDCDRNDYRKAREAALIYRKKSMVKEILVYCIQITSWLVRTTAAFKTTKYDTTLDTLRQKCGLIFEGIHYAQEISFLVQSITSLHLLMHESISQQMFHAVGKLLEYLQAVQNFFDSNQQAIAETNQFIIQHLQHKIFVILITDAKQAKLKKSEIFIDKLSAFHVIERCMSGPANRTRLTIARLAISISDPRQTLSPESYEKIRILLDQLDILANLQTKLRNLSDTQFLYWHQTFFSLYVSDFYDHNTAQNNLKYLLQSAATFASDNTTQNAIAVLSKNHRKSFQDKLLHQISNHIETYIRLDYYGKTYQIEPYNPFTDTTMALSNIYSLVRMEPILVGGQYLSVKEHVERYLSNTYYTLASISQHDWKIYKEMRCHAACFQIETIEDQLPKQTLEQGLDVLEIMHNIEHFVSHYVYNLNFQIFIEQSSTNHFLNTVNIGHIANSLRRHGNGIINTTVNYTYQFLRQKFFAFSDFLYDEQIKARLTSDAKYFLENGEALNQTYDYNRANAFNRKIKNLGLSEAGETYMDLFRKLICHIGNAMGYVRMIRSGALHECTEATVYLPVIDGDLNFSKFCKEESLHDLTISAAEILENDINNLCKNYRIDTNYFRLLVNAFLSLRHAENIHLQNFYMIVPPLTINFVEYILKAKEKITKKDKVGALFTDDGFAMGIAYILKLLDQTTKFNSLHWFRSVKNKYSQEIEKLDTQQQQSDKTTDGDKLQQTFALTRKRLNMVQQEFDLLFCNLSSAKIFFNDAVD

Target ORF information:

RefSeq Version	XM_001656301.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL013108-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001656301.1

ORF Insert Sequence:

ATGTTGGAAG CCATGTTCAA TGCCAAGCAG AAGTTGGTTC TCGAGTCGGC AAACGTTTTG 
TCGGATAAGT TTATGAAATA TATCAGACAA CGTTTAGTAG AGGTTGGCAG TTACAATGCC 
AATCTGTCTA GCATTGACGA GACTACCAAC GTCGTTAAGC TCAATGCCAT GATTAACTTG 
TATCATTACA TATTCGTGAG CATTGACAAC AAGGTGGTTA AAAGTTTGAT GGATATCAAT 
GATAAGTTTT GTGGCGTGCC GCTTATCGGG AAAGTGATGT GGATTCCGGA CGACTTTTTC 
CGGAAATATG GCCACAAGTC GATTCGCGAT TGCGATCGCA ACGATTACCG CAAGGCACGT 
GAGGCTGCCC TTATCTACCG GAAGAAAAGC ATGGTCAAGG AAATCCTCGT ATATTGTATC 
CAGATAACGA GCTGGTTGGT TCGGACCACG GCTGCGTTCA AGACGACAAA ATACGACACC 
ACGTTGGACA CTTTGCGGCA AAAGTGCGGC CTAATCTTCG AGGGTATTCA CTATGCGCAG 
GAGATCAGCT TTCTGGTGCA ATCGATAACG TCGCTGCATT TGCTGATGCA CGAATCCATC 
AGCCAGCAAA TGTTTCATGC GGTGGGAAAA TTGCTAGAAT ATCTACAGGC CGTCCAGAAC 
TTTTTCGATA GCAATCAGCA AGCCATCGCA GAAACGAACC AGTTCATCAT TCAACATTTG 
CAGCATAAGA TCTTCGTTAT TTTAATAACC GATGCTAAAC AAGCCAAGCT AAAAAAGAGC 
GAAATTTTCA TCGATAAACT GTCGGCCTTC CACGTGATTG AACGCTGTAT GAGTGGACCG 
GCGAATCGCA CCCGTTTGAC CATCGCACGT TTGGCCATTT CCATAAGTGA TCCGCGTCAA 
ACTCTCTCTC CCGAGAGCTA CGAAAAAATC CGCATACTTT TGGATCAACT GGACATTCTG 
GCCAATCTGC AAACTAAGCT TCGAAACCTC AGCGATACAC AATTTCTGTA CTGGCACCAA 
ACGTTCTTTT CGCTGTACGT TTCCGATTTC TACGACCATA ATACAGCTCA GAATAACTTG 
AAATATTTGT TGCAATCAGC TGCTACCTTT GCCTCCGATA ATACCACGCA AAATGCCATA 
GCTGTTTTAT CGAAAAACCA TCGGAAATCA TTTCAAGATA AATTGCTACA TCAAATAAGC 
AATCACATTG AAACGTACAT CCGGCTAGAC TACTATGGGA AAACGTACCA GATTGAACCG 
TACAATCCTT TCACCGATAC CACAATGGCA CTGAGTAACA TCTACTCTCT GGTGAGGATG 
GAACCCATCC TTGTCGGAGG TCAGTACCTA AGCGTGAAGG AACACGTTGA ACGATATCTG 
AGCAACACGT ACTACACGTT GGCGTCGATT TCGCAACATG ATTGGAAGAT CTACAAGGAA 
ATGCGATGTC ACGCGGCCTG TTTCCAGATT GAAACCATCG AGGATCAGCT ACCTAAGCAA 
ACGTTAGAGC AGGGGCTGGA TGTGCTGGAG ATTATGCACA ACATCGAACA TTTTGTAAGC 
CACTACGTGT ACAATTTGAA CTTCCAGATA TTCATCGAAC AGTCGAGTAC CAACCACTTC 
CTGAACACGG TCAACATTGG ACACATTGCG AATTCGCTGC GGCGCCACGG CAACGGCATC 
ATTAACACTA CGGTCAACTA CACATACCAG TTTCTTCGGC AAAAGTTCTT CGCCTTTTCG 
GACTTTTTGT ACGACGAACA GATCAAAGCT CGTTTAACCA GCGACGCCAA ATATTTCCTA 
GAAAATGGTG AGGCGCTCAA TCAAACGTAT GATTACAATC GAGCCAATGC TTTCAATCGC 
AAAATAAAGA ACCTAGGCCT GTCGGAAGCA GGCGAAACCT ATATGGATCT GTTCAGGAAG 
TTGATTTGTC ACATTGGCAA TGCCATGGGA TACGTTCGGA TGATACGTTC CGGAGCGCTG 
CACGAATGTA CGGAAGCTAC CGTATACTTG CCCGTCATAG ACGGGGATCT CAATTTCTCA 
AAATTTTGCA AAGAAGAATC CTTGCACGAT TTGACGATCT CAGCGGCGGA GATCTTGGAA 
AATGATATCA ACAACTTGTG TAAAAACTAT CGAATTGATA CCAACTATTT CCGTTTGCTG 
GTGAATGCCT TTCTGTCTTT ACGGCACGCG GAAAATATTC ATCTGCAAAA CTTCTACATG 
ATTGTACCCC CTCTGACCAT CAACTTTGTG GAGTACATTT TGAAGGCGAA GGAGAAAATT 
ACCAAGAAGG ATAAAGTAGG TGCCTTATTT ACCGACGATG GATTTGCCAT GGGAATTGCT 
TACATCCTCA AACTGTTGGA CCAAACTACT AAGTTCAACT CCCTGCATTG GTTCCGTTCG 
GTGAAGAACA AATACAGCCA GGAAATCGAA AAACTAGATA CCCAACAGCA GCAATCGGAC 
AAAACGACCG ACGGCGACAA ATTGCAGCAA ACATTCGCTT TGACGCGAAA ACGGCTCAAC 
ATGGTCCAGC AGGAATTTGA TCTTCTGTTT TGTAATTTGA GCAGTGCAAA GATATTCTTC 
AACGACGCTG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL013108 ( XM_021849203.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021704895.1 Aedes aegypti WASH complex subunit 4 (LOC5577249), mRNA.

Price & Availability↑

CloneID	OAe132244
Clone ID Related Accession (Same CDS sequence)	XM_021849203.1
Accession Version	XM_021849203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	WASH complex subunit 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGGA GCCAGCAAAA CCTGAATTAT TACGGTGCAA CGCAAATCAA AGAATTTGGA 
CTGTTCCTGG AGGATTACGA CGTTAGATTG CAGCGGCTCT CATCGACAAG CGGTGGCGAT 
GATCCTCCAG TTCCCACGGT TTCAATCCAG CTGGATTCAT CGAAGGAAAA TCTGGGAAGC 
ATCCAGCTGA TCGACACCGA CAATAAAATC CTGAACAAAA TCCTCTACTC GTTCAGTGCC 
CTGTGCCATG AAGTGGACCG ACTGAGGGAA GAATTCGAGC TCATCGTCCG GGAGTTTCTG 
TGCTTCGATG AAGCGCTGGA CGACAATACG GAACAGCTGC GGGAGGACAG CACGATTGCC 
GTGGACCGAT CCTCGCTGCT GGCCATTAGT GGCAAAATTG AACTGCTGGC CAGGATTAAG 
TGCTACTTTG AACGGATCGT GGAGGTGGCC GTGGTCATTG TGCTTCAGCT GGGGGCGCTG 
TTTGACCCGT CGAATAAGCT TGGACACTTC AATCACTCCA ATTGGGAGAT GGACCTAATG 
TTCAATTACT TATCGGATTT GATGTATATG CCATTGCTGT TCGACTGCGT CCTCGGAAGT 
TCAATGTTCA AAACGTATTG GAAGTTTTAC ATGAAACAGA TGCGAGCTCT GAAAATGAAT 
CCACAGAAGC TGTCGAAACC CATCAAAATG GACCAAATCT ACGATTTGAA CAAAGCGATA 
GATGAAATTT CGGTGTGGCT TGATGAGAAT GCCTATAAGC ACATGTTGGA AGCCATGTTC 
AATGCCAAGC AGAAGTTGGT TCTCGAGTCG GCAAACGTTT TGTCGGATAA GTTTATGAAA 
TATATCAGAC AACGTTTAGT AGAGGTTGGC AGTTATAATG CCAATCTGTC TAGCATTGAC 
GAGACTACCA ACGTCGTTAA GCTCAATGCC ATGATTAACT TGTATCATTA CATATTCGTG 
AGCATTGACA ACAAGGTGGT TAAAAGTTTG ATGGATATCA ATGATAAGTT TTGTGGCGTG 
CCGCTTATCG GGAAAGTGAT GTGGATTCCG GACGACTTTT TCCGGAAATA TGGCCACAAG 
TCGATTCGCG ATTGCGATCG CAACGATTAC CGCAAGGCAC GTGAGGCTGC CCTTATCTAC 
CGGAAGAAAA GCATGGTCAA GGAAATCCTC GTATATTGTA TCCAGATAAC GAGCTGGTTG 
GTTCGGACCA CGGCTGCGTT CAAGACGACA AAATACGACA CCACGTTGGA CACTTTGCGG 
CAAAAGTGCG GCCTAATCTT CGAGGGTATT CACTACGCCC AGGAGATCAG CTTTCTGGTG 
CAATCGATAA CGTCGCTGCA TTTGCTGATG CACGAATCCA TCAGCCAGCA AATGTTTCAT 
GCGGTGGGAA AATTGCTAGA ATATCTACAG GCCGTTCAGA ACTTTTTCGA TAGCAATCAG 
CAAGCCATCG CAGAAACGAA CCAGTTCATC ATTCAACATT TGCAGCATAA GATCTTCGTT 
ATTGTGGCAC ATTCAAAGAA AAAGTTAATA ACCGATGCTA AACAAGCCAA GCTAAAAAAG 
AGCGAAATTT TCATCGATAA ACTGTCGGCC TTCCACGTCA TAGAACGCTG TATGAGTGGA 
CCGGCGAATC GCACCCGTTT GACCATCGCA CGTTTGGCCA TTTCCATAAG TGATCCGCGT 
CAAACTCTCT CTCCCGAGAG CTACGAAAAA ATCCGCATAC TTTTGGATCA ACTGGACATT 
CTGGCCAATC TGCAAACTAA GCTTCGAAAC CTCAGCGATA CACAATTTCT GTACTGGCAC 
CAAACGTTCT TTTCGCTGTA CGTTTCCGAT TTCTACGACC ATAATACAGC TCAGAACAAC 
TTGAAATATT TGTTGCAATC AGCTGCTACC TGTTGCGAAT CTGTAAGCCA AGTTGCCTCC 
GATAATACCA CGCAAAATGC CATAGCTGTT TTATCGAAAA ACCATCGGAA ATCATTTCAA 
GATAAATTGC TACATCAAAT AAGCAATCAC ATTGAAACGT ACATCCGGCT AGACTACTAT 
GGGAAAACGT ACCAGATTGA ACCGTACAAT CCTTTCACCG ATACCACAAT GGCACTGAGT 
AACATCTACT CTCTGGTGAG GATGGAACCC ATCCTTGTCG GAGGTCAGTA CCTAAGCGTG 
AAGGAACACG TTGAACGATA TCTGAGCAAC ACGTACTACA CGTTGGCGTC GATTTCGCAA 
CATGATTGGA AGATCTACAA GGAAATGCGA TGTCACGCGG CCTGTTTCCA GATTGAAACC 
ATCGAGGATC AGCTACCTAA GCAAACGTTA GAGCAGGGGC TGGATGTGCT GGAGATTATG 
CACAACATCG AACATTTTGT AAGCCACTAC GTGTACAATT TGAACTTCCA GATATTCATC 
GAACAGTCGA GCACCAACCA CTTCCTGAAC ACGGTCAACA TTGGACACAT TGCGAATTCG 
CTGCGGCGCC ACGGCAACGG CATCATTAAC ACTACGGTCA ACTACACATA CCAGTTTCTT 
CGGCAAAAGT TCTTCGCCTT TTCGGACTTT TTGTACGATG AACAGATCAA AGCTCGTTTA 
ACCAGCGACG CTAAATATTT CCTAGAAAAT GGCGAGGCGC TCAACCAAAC GTATGATTAC 
AATCGAGCCA ATGCCTTCAA TCGCAAAATA AAGAACCTAG GCCTGTCGGA AGCAGGCGAA 
ACCTATATGG ATCTGTTCAG GAAGTTGATT TGTCACATTG GCAATGCCAT GGGATACGTT 
CGGATGATAC GTTCCGGAGC GCTGCATGAA TGTACGGAAG CTACCGTATA CTTGCCCGTC 
ATAGACGGGG ATCTCAATTT CTCAAAATTT TGCAAAGAAG AATCCTTGCA CGAGTTGACG 
ATCTCAGCGG CGGAAATCTT GGAAAATGAT ATCAACAACT TGTGTAAAAA CTATCGAATC 
GATACCAACT ATTTCCGTTT GCTGGTGAAT GCCTTTCTGT CTTTACGGCA CGCGGAAAAT 
ATTCATCTGC AAAACTTCTA CATGATTGTA CCCCCTCTGA CCATCAACTT TGTGGAGTAC 
ATTTTGAAGG CGAAGGAGAA AATTACCAAG AAGGATAAAG TAGGTGCCTT ATTTACCGAC 
GATGGATTTG CCATGGGAAT TGCTTACATC CTCAAACTGT TGGACCAAAC TACTAAGTTC 
AACTCCCTGC ATTGGTTCCG TTCGGTGAAG AACAAATACA GCCAGGAAAT CGAAAAACTA 
GATACCCAAC AGCAGCAATC GGACAAAACG ACCGACGGCG ACAAATTGCA GCAAACATTC 
GCTTTGACGC GAAAACGGCT CAACATGGTC CAGCAGGAAT TTGATCTTCT GTTTTGTAAT 
TTGAGCAGTG CAAAGATATT CTTCAACGAC GCTGTGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021704895.1
CDS	179..3580
Translation	MHRSQQNLNYYGATQIKEFGLFLEDYDVRLQRLSSTSGGDDPPVPTVSIQLDSSKENLGSIQLIDTDNKILNKILYSFSALCHEVDRLREEFELIVREFLCFDEALDDNTEQLREDSTIAVDRSSLLAISGKIELLARIKCYFERIVEVAVVIVLQLGALFDPSNKLGHFNHSNWEMDLMFNYLSDLMYMPLLFDCVLGSSMFKTYWKFYMKQMRALKMNPQKLSKPIKMDQIYDLNKAIDEISVWLDENAYKHMLEAMFNAKQKLVLESANVLSDKFMKYIRQRLVEVGSYNANLSSIDETTNVVKLNAMINLYHYIFVSIDNKVVKSLMDINDKFCGVPLIGKVMWIPDDFFRKYGHKSIRDCDRNDYRKAREAALIYRKKSMVKEILVYCIQITSWLVRTTAAFKTTKYDTTLDTLRQKCGLIFEGIHYAQEISFLVQSITSLHLLMHESISQQMFHAVGKLLEYLQAVQNFFDSNQQAIAETNQFIIQHLQHKIFVIVAHSKKKLITDAKQAKLKKSEIFIDKLSAFHVIERCMSGPANRTRLTIARLAISISDPRQTLSPESYEKIRILLDQLDILANLQTKLRNLSDTQFLYWHQTFFSLYVSDFYDHNTAQNNLKYLLQSAATCCESVSQVASDNTTQNAIAVLSKNHRKSFQDKLLHQISNHIETYIRLDYYGKTYQIEPYNPFTDTTMALSNIYSLVRMEPILVGGQYLSVKEHVERYLSNTYYTLASISQHDWKIYKEMRCHAACFQIETIEDQLPKQTLEQGLDVLEIMHNIEHFVSHYVYNLNFQIFIEQSSTNHFLNTVNIGHIANSLRRHGNGIINTTVNYTYQFLRQKFFAFSDFLYDEQIKARLTSDAKYFLENGEALNQTYDYNRANAFNRKIKNLGLSEAGETYMDLFRKLICHIGNAMGYVRMIRSGALHECTEATVYLPVIDGDLNFSKFCKEESLHELTISAAEILENDINNLCKNYRIDTNYFRLLVNAFLSLRHAENIHLQNFYMIVPPLTINFVEYILKAKEKITKKDKVGALFTDDGFAMGIAYILKLLDQTTKFNSLHWFRSVKNKYSQEIEKLDTQQQQSDKTTDGDKLQQTFALTRKRLNMVQQEFDLLFCNLSSAKIFFNDAVD

Target ORF information:

RefSeq Version	XM_021849203.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti WASH complex subunit 4 (LOC5577249), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021849203.1

ORF Insert Sequence:

ATGCACCGGA GCCAGCAAAA CCTGAATTAT TACGGTGCAA CGCAAATCAA AGAATTTGGA 
CTGTTCCTGG AGGATTACGA CGTTAGATTG CAGCGGCTCT CATCGACAAG CGGTGGCGAT 
GATCCTCCAG TTCCCACGGT TTCAATCCAG CTGGATTCAT CGAAGGAAAA TCTGGGAAGC 
ATCCAGCTGA TCGACACCGA CAATAAAATC CTGAACAAAA TCCTCTACTC GTTCAGTGCC 
CTGTGCCATG AAGTGGACCG ACTGAGGGAA GAATTCGAGC TCATCGTCCG GGAGTTTCTG 
TGCTTCGATG AAGCGCTGGA CGACAATACG GAACAGCTGC GGGAGGACAG CACGATTGCC 
GTGGACCGAT CCTCGCTGCT GGCCATTAGT GGCAAAATTG AACTGCTGGC CAGGATTAAG 
TGCTACTTTG AACGGATCGT GGAGGTGGCC GTGGTCATTG TGCTTCAGCT GGGGGCGCTG 
TTTGACCCGT CGAATAAGCT TGGACACTTC AATCACTCCA ATTGGGAGAT GGACCTAATG 
TTCAATTACT TATCGGATTT GATGTATATG CCATTGCTGT TCGACTGCGT CCTCGGAAGT 
TCAATGTTCA AAACGTATTG GAAGTTTTAC ATGAAACAGA TGCGAGCTCT GAAAATGAAT 
CCACAGAAGC TGTCGAAACC CATCAAAATG GACCAAATCT ACGATTTGAA CAAAGCGATA 
GATGAAATTT CGGTGTGGCT TGATGAGAAT GCCTATAAGC ACATGTTGGA AGCCATGTTC 
AATGCCAAGC AGAAGTTGGT TCTCGAGTCG GCAAACGTTT TGTCGGATAA GTTTATGAAA 
TATATCAGAC AACGTTTAGT AGAGGTTGGC AGTTATAATG CCAATCTGTC TAGCATTGAC 
GAGACTACCA ACGTCGTTAA GCTCAATGCC ATGATTAACT TGTATCATTA CATATTCGTG 
AGCATTGACA ACAAGGTGGT TAAAAGTTTG ATGGATATCA ATGATAAGTT TTGTGGCGTG 
CCGCTTATCG GGAAAGTGAT GTGGATTCCG GACGACTTTT TCCGGAAATA TGGCCACAAG 
TCGATTCGCG ATTGCGATCG CAACGATTAC CGCAAGGCAC GTGAGGCTGC CCTTATCTAC 
CGGAAGAAAA GCATGGTCAA GGAAATCCTC GTATATTGTA TCCAGATAAC GAGCTGGTTG 
GTTCGGACCA CGGCTGCGTT CAAGACGACA AAATACGACA CCACGTTGGA CACTTTGCGG 
CAAAAGTGCG GCCTAATCTT CGAGGGTATT CACTACGCCC AGGAGATCAG CTTTCTGGTG 
CAATCGATAA CGTCGCTGCA TTTGCTGATG CACGAATCCA TCAGCCAGCA AATGTTTCAT 
GCGGTGGGAA AATTGCTAGA ATATCTACAG GCCGTTCAGA ACTTTTTCGA TAGCAATCAG 
CAAGCCATCG CAGAAACGAA CCAGTTCATC ATTCAACATT TGCAGCATAA GATCTTCGTT 
ATTGTGGCAC ATTCAAAGAA AAAGTTAATA ACCGATGCTA AACAAGCCAA GCTAAAAAAG 
AGCGAAATTT TCATCGATAA ACTGTCGGCC TTCCACGTCA TAGAACGCTG TATGAGTGGA 
CCGGCGAATC GCACCCGTTT GACCATCGCA CGTTTGGCCA TTTCCATAAG TGATCCGCGT 
CAAACTCTCT CTCCCGAGAG CTACGAAAAA ATCCGCATAC TTTTGGATCA ACTGGACATT 
CTGGCCAATC TGCAAACTAA GCTTCGAAAC CTCAGCGATA CACAATTTCT GTACTGGCAC 
CAAACGTTCT TTTCGCTGTA CGTTTCCGAT TTCTACGACC ATAATACAGC TCAGAACAAC 
TTGAAATATT TGTTGCAATC AGCTGCTACC TGTTGCGAAT CTGTAAGCCA AGTTGCCTCC 
GATAATACCA CGCAAAATGC CATAGCTGTT TTATCGAAAA ACCATCGGAA ATCATTTCAA 
GATAAATTGC TACATCAAAT AAGCAATCAC ATTGAAACGT ACATCCGGCT AGACTACTAT 
GGGAAAACGT ACCAGATTGA ACCGTACAAT CCTTTCACCG ATACCACAAT GGCACTGAGT 
AACATCTACT CTCTGGTGAG GATGGAACCC ATCCTTGTCG GAGGTCAGTA CCTAAGCGTG 
AAGGAACACG TTGAACGATA TCTGAGCAAC ACGTACTACA CGTTGGCGTC GATTTCGCAA 
CATGATTGGA AGATCTACAA GGAAATGCGA TGTCACGCGG CCTGTTTCCA GATTGAAACC 
ATCGAGGATC AGCTACCTAA GCAAACGTTA GAGCAGGGGC TGGATGTGCT GGAGATTATG 
CACAACATCG AACATTTTGT AAGCCACTAC GTGTACAATT TGAACTTCCA GATATTCATC 
GAACAGTCGA GCACCAACCA CTTCCTGAAC ACGGTCAACA TTGGACACAT TGCGAATTCG 
CTGCGGCGCC ACGGCAACGG CATCATTAAC ACTACGGTCA ACTACACATA CCAGTTTCTT 
CGGCAAAAGT TCTTCGCCTT TTCGGACTTT TTGTACGATG AACAGATCAA AGCTCGTTTA 
ACCAGCGACG CTAAATATTT CCTAGAAAAT GGCGAGGCGC TCAACCAAAC GTATGATTAC 
AATCGAGCCA ATGCCTTCAA TCGCAAAATA AAGAACCTAG GCCTGTCGGA AGCAGGCGAA 
ACCTATATGG ATCTGTTCAG GAAGTTGATT TGTCACATTG GCAATGCCAT GGGATACGTT 
CGGATGATAC GTTCCGGAGC GCTGCATGAA TGTACGGAAG CTACCGTATA CTTGCCCGTC 
ATAGACGGGG ATCTCAATTT CTCAAAATTT TGCAAAGAAG AATCCTTGCA CGAGTTGACG 
ATCTCAGCGG CGGAAATCTT GGAAAATGAT ATCAACAACT TGTGTAAAAA CTATCGAATC 
GATACCAACT ATTTCCGTTT GCTGGTGAAT GCCTTTCTGT CTTTACGGCA CGCGGAAAAT 
ATTCATCTGC AAAACTTCTA CATGATTGTA CCCCCTCTGA CCATCAACTT TGTGGAGTAC 
ATTTTGAAGG CGAAGGAGAA AATTACCAAG AAGGATAAAG TAGGTGCCTT ATTTACCGAC 
GATGGATTTG CCATGGGAAT TGCTTACATC CTCAAACTGT TGGACCAAAC TACTAAGTTC 
AACTCCCTGC ATTGGTTCCG TTCGGTGAAG AACAAATACA GCCAGGAAAT CGAAAAACTA 
GATACCCAAC AGCAGCAATC GGACAAAACG ACCGACGGCG ACAAATTGCA GCAAACATTC 
GCTTTGACGC GAAAACGGCT CAACATGGTC CAGCAGGAAT TTGATCTTCT GTTTTGTAAT 
TTGAGCAGTG CAAAGATATT CTTCAACGAC GCTGTGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL013108 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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