AAEL_AAEL011649 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL011649
Entrez Gene ID	5575094
Full Name	helicase POLQ-like
Synonyms	AAEL011649
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001661768.2	XP_001661818.2	helicase POLQ-like isoform X1
XM_021850281.1	XP_021705973.1	helicase POLQ-like isoform X2
XM_001661768.1	XP_001661818.1	AAEL011649-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL011649 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL011649 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe10144	XM_001661768.1 Latest version!	Aedes aegypti AAEL011649-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe126153	XM_001661768.2 Latest version!	Aedes aegypti helicase POLQ-like (LOC5575094), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe126154	XM_021850281.1 Latest version!	Aedes aegypti helicase POLQ-like (LOC5575094), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL011649 ( XM_001661768.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001661818.1 Aedes aegypti AAEL011649-RA partial mRNA.

Price & Availability↑

CloneID	OAe10144
Clone ID Related Accession (Same CDS sequence)	XM_001661768.1
Accession Version	XM_001661768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL011649-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811349). COMPLETENESS: incomplete on the 5' end.

ATGACTCCCA GAAGGAAAAA GATCCGCCGA AACCCAGGAC TGATAGTGTT GGATATGACC 
AAGCCAGAAG TTGACTCAGT AATTGCCCCA GCACCAAAAT CGAAGCCCCC CTCGGACGAT 
ACGATGCGAG CCGAAACTTC CCACCGAATG AACCAAACCC GGACAATTCC GGTGATCCCT 
TCCCAGACGG CGGTCGCAGT GGCAGATAGA CGTCCAACAA TCCCCCCGCC AACAACCATG 
ATCCCCAGAG ATCCCACCGT GGATCTGTTC GAGTGGGACT CCCAGGCTTT CGGCACACAA 
GTCGAACGGA TTGTGAGCCG AAACGTTGCC TCGAGGAGTT CCCCTCCACG CGATCTTGTA 
GACGACTACG ATGATTTCCC TTGCTATCAG CAAACCAACG CTAACCAGCC GGAGGTTTCC 
GAGCTGAACG ACAACGACGA CAATCTGAAT TGCTCGCAAT TGTTCATGGA GGAAATCGCG 
CGGGAACCTC ACCGCCCTTT GACCCAGATG AGATACTCCC AGCAAAGGGA TCCTCACTCG 
GAACGACCGA ACGCGAACGC CACCACATAT TCGCAGTACA TCCGTAATGA GTACACCATG 
CTGGAGGAGA ACCGTGCCAA CGTGATCGAG GACGAGGTGG ACATCGCCCT GGCCCAACAG 
TTCGATTCCA GTCAGTACCG GACGGAGCAG GAAAGCAAAT TGAGGATATG TGAACGCGAA 
ATGCTCGACA TGAGCAACAT CAACTGGGAC GAGAGCGTTG CCATTGCGGG GGAGAGGCCA 
TCGACTTCCA CGTCTACGGC CAAGGCTCGT CTGGATGGGA TCTTTGGGAA GACGGACAGG 
GCGAACGGAA GGGTCGTGGA ATCGCCGAAG CTGGTGGCCA AAAAGTCGGG GCCGCTGGTG 
TCGTCGCACT TCCAGGATTT GGGCCCATTC TATGGATTGC CGAACAAGAT CCGCGATTTG 
CTTCGGGAGT TTCGCGGGAT TACGGAGATG TATGATTGGC AGAAAGAGTG CCTCTCGCTG 
CCGGCCATCG TCGAGCGAAA GAATCTGATC TACGCCCTTC CGACGAGCGG TGGTAAAACC 
CTGGTCGCCG AAATTATCAT GTTCCGGGAG GTACTGCTCC GGAATCGCAG CGTAATGTTC 
ATCGTCCCGT ACGTGTCGTT GGCCCAGGAA AAGCTAGCCG CCCTGTCGCC CTTTGCCGTG 
GCCACGCAGT TCCTCGTGGA GGAATACAGC GGTGGCAAGG GAATGATTCC ACCCCGGAAA 
CGCCGAAGGA AGCAGTCCAT CTTCGTGTGC ACTATCGAGA AGGCACTGGT CTTGCTGGAT 
AGTCTGATCG AGGCCGATCG GGCGAACGAA ATAGGATTGA TTTGCATAGA TGAGTTGCAC 
ATGATCGGTG AACCCAATCG TGGGGTCAAC CTGGAAGTGC TGATCACCAA AGTGCAAGCC 
ATCCAGGCCG GGATTCAGAT CATCGGGATG AGTGCCACGA TCGGAAATCT GGCGGAGATA 
GCCAAGTTCA TGTCGGCGGA CATTTTCAGC CAGGACTTCC GGCCGGTGGA GTTGAAAGAG 
TTTGTGAAAT GTGGTAGCGA TATGTTGGAG ATCAAGCACG GCGCTCGGAC TTTGGAGGAA 
GCATTCGTTA ACAAGCGAAC GGTTAATTAC AAATATTCCG ACACGGATAC CCGAATCGAT 
CCGGACCATA TAATTGGCCT AGTAATGGAA ACTATCCCGA ATGATCCCTG CCTGATCTTT 
TGTCCTACGA AACGTCGATG TGAACAGCTA TGTTCGTTTC TGATTGAGCA CTTGCCGGAT 
CGAGTGGCGG ACTATCGTGC CCCAGAGCGG AAAACATTGA TCGACCTTCT CGAAAATGAC 
GGATCGACGG CTACTTTGTT GCCAGCGGCC TTCCGATTAG GCATTGCCTA CCACCATTCC 
GGACTGACCT TCGACGAACG AAAACTCATT GAGGATGCTT TCCGGGCGAA AGTGCTCTCG 
CTCATCATTT GCACTTCCAC CCTGGCTGCC GGAGTTAATC TACCGGCCAA ACGGGTGATC 
ATACGATCGC CATACATTGC CACCGAGTTC CTGACCCTAA GTCGGTACAA ACAAATGGTT 
GGTCGAGCTG GTCGGGCCGG ATTCGGCAAT GAAGGCGGCG AAAGCATTCT GATCTGTGCC 
CAAAAAGACA TCGCCTTGGT TTGCAACCTT CTTTGCTCTC CGATGGACGA AGCGAACTCC 
TCACTTCATG CGGATAACTT CATACATCTT AAGAACCTAA TACTCAGTGC AATCGGTTTA 
GGCATCTGTT CCACTCGGAA CGACGTCCTA AAGTTTGTCG CCAAAACCCT GCTGGCCATC 
CAGACTGAGC GATTGGGAGT AAACCTCCGG AGTGTCACGG ATGCCGCGAT CACCGCCTTG 
TACAAAGAAA ATGCCATCAA GGCCAAAAGC GACGGTTGTT TGCGAAATCC GGCGAACATG 
ACGGTACAGT TCGATGCGAC CGATCAGACC CAGTACGATT TTGAACCAGG AGGCAACGGT 
TTCCGTGAGC TGGAAGTTCG CCGAGGGCTG GAAGGTTCTT CGCAAGACGG TAAACTGGTG 
AAGATTGTGA AGAAGAACTC CAGGTTGGAG GTCAATCTAC TGGGGAAGGC TTCCATTCGG 
GCTGGATTCC ACATGGAACG AGCAACGCGG GCTTACGATG AATTGAAACG AGTCGGCGAA 
CGACTCTATG TCAATGATGA ACTGCACCTG CTGTGGGTCA TCGTAATGGA GGACGGCGGT 
GAAATTCGAC CGAAGGAAGA CGATTTCATT TCATTCTTCA ACAAGCTTTC GCCGGAAGAG 
ACTAGGATTG CAAAAAGATT TTTCATCAGT GACTACACGA TATCGATGAT CCTCAGCAGA 
CGGTTAGATA AGATAGACAT GAACTTGTTG CGACGCTTCT TCCGAACTCT GATCGTGTAC 
AACCTTTGGA ATCTCATCCC ACCGCAGGAG GTGGCGGTCA GGTTCACCGT GGACGCAGGC 
TCGGTGCAGA CCCTGATGAC CAATGCGGCC GCGACGGCCT CCAGTCTACA GCGGTTCTGC 
GAACAGCTGC CGGAATTTTG GGCATTCGAT TCGCTGCTGA AACGTATGGT GCAACGGCTG 
GCACATTGCT GTACGGCCGA ACTGATCCCT CTGATGGAAC TGCCGGCGGT GAAAATTGGA 
CGGGCACGGC AACTTTATCG AGCCGGATAC ACCTCTTTGT CTTCGATAGC GAAAGCGAAA 
TCAAGTGATC TGGTGTCTAC GGTCCAGCAC ATGAACTACC GGACGGCGAA TCAGTTGATT 
TTATCGGCGA AGGCCAAACT AATGGAGGAA TTTGAGGAGC TCCGGGATCG AATGGATGAC 
TATTTTCATC TCACATCCAT CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001661818.1
CDS	1..3387
Translation	MTPRRKKIRRNPGLIVLDMTKPEVDSVIAPAPKSKPPSDDTMRAETSHRMNQTRTIPVIPSQTAVAVADRRPTIPPPTTMIPRDPTVDLFEWDSQAFGTQVERIVSRNVASRSSPPRDLVDDYDDFPCYQQTNANQPEVSELNDNDDNLNCSQLFMEEIAREPHRPLTQMRYSQQRDPHSERPNANATTYSQYIRNEYTMLEENRANVIEDEVDIALAQQFDSSQYRTEQESKLRICEREMLDMSNINWDESVAIAGERPSTSTSTAKARLDGIFGKTDRANGRVVESPKLVAKKSGPLVSSHFQDLGPFYGLPNKIRDLLREFRGITEMYDWQKECLSLPAIVERKNLIYALPTSGGKTLVAEIIMFREVLLRNRSVMFIVPYVSLAQEKLAALSPFAVATQFLVEEYSGGKGMIPPRKRRRKQSIFVCTIEKALVLLDSLIEADRANEIGLICIDELHMIGEPNRGVNLEVLITKVQAIQAGIQIIGMSATIGNLAEIAKFMSADIFSQDFRPVELKEFVKCGSDMLEIKHGARTLEEAFVNKRTVNYKYSDTDTRIDPDHIIGLVMETIPNDPCLIFCPTKRRCEQLCSFLIEHLPDRVADYRAPERKTLIDLLENDGSTATLLPAAFRLGIAYHHSGLTFDERKLIEDAFRAKVLSLIICTSTLAAGVNLPAKRVIIRSPYIATEFLTLSRYKQMVGRAGRAGFGNEGGESILICAQKDIALVCNLLCSPMDEANSSLHADNFIHLKNLILSAIGLGICSTRNDVLKFVAKTLLAIQTERLGVNLRSVTDAAITALYKENAIKAKSDGCLRNPANMTVQFDATDQTQYDFEPGGNGFRELEVRRGLEGSSQDGKLVKIVKKNSRLEVNLLGKASIRAGFHMERATRAYDELKRVGERLYVNDELHLLWVIVMEDGGEIRPKEDDFISFFNKLSPEETRIAKRFFISDYTISMILSRRLDKIDMNLLRRFFRTLIVYNLWNLIPPQEVAVRFTVDAGSVQTLMTNAAATASSLQRFCEQLPEFWAFDSLLKRMVQRLAHCCTAELIPLMELPAVKIGRARQLYRAGYTSLSSIAKAKSSDLVSTVQHMNYRTANQLILSAKAKLMEEFEELRDRMDDYFHLTSIS

Target ORF information:

RefSeq Version	XM_001661768.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL011649-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001661768.1

ORF Insert Sequence:

ATGACTCCCA GAAGGAAAAA GATCCGCCGA AACCCAGGAC TGATAGTGTT GGATATGACC 
AAGCCAGAAG TTGACTCAGT AATTGCCCCA GCACCAAAAT CGAAGCCCCC CTCGGACGAT 
ACGATGCGAG CCGAAACTTC CCACCGAATG AACCAAACCC GGACAATTCC GGTGATCCCT 
TCCCAGACGG CGGTCGCAGT GGCAGATAGA CGTCCAACAA TCCCCCCGCC AACAACCATG 
ATCCCCAGAG ATCCCACCGT GGATCTGTTC GAGTGGGACT CCCAGGCTTT CGGCACACAA 
GTCGAACGGA TTGTGAGCCG AAACGTTGCC TCGAGGAGTT CCCCTCCACG CGATCTTGTA 
GACGACTACG ATGATTTCCC TTGCTATCAG CAAACCAACG CTAACCAGCC GGAGGTTTCC 
GAGCTGAACG ACAACGACGA CAATCTGAAT TGCTCGCAAT TGTTCATGGA GGAAATCGCG 
CGGGAACCTC ACCGCCCTTT GACCCAGATG AGATACTCCC AGCAAAGGGA TCCTCACTCG 
GAACGACCGA ACGCGAACGC CACCACATAT TCGCAGTACA TCCGTAATGA GTACACCATG 
CTGGAGGAGA ACCGTGCCAA CGTGATCGAG GACGAGGTGG ACATCGCCCT GGCCCAACAG 
TTCGATTCCA GTCAGTACCG GACGGAGCAG GAAAGCAAAT TGAGGATATG TGAACGCGAA 
ATGCTCGACA TGAGCAACAT CAACTGGGAC GAGAGCGTTG CCATTGCGGG GGAGAGGCCA 
TCGACTTCCA CGTCTACGGC CAAGGCTCGT CTGGATGGGA TCTTTGGGAA GACGGACAGG 
GCGAACGGAA GGGTCGTGGA ATCGCCGAAG CTGGTGGCCA AAAAGTCGGG GCCGCTGGTG 
TCGTCGCACT TCCAGGATTT GGGCCCATTC TATGGATTGC CGAACAAGAT CCGCGATTTG 
CTTCGGGAGT TTCGCGGGAT TACGGAGATG TATGATTGGC AGAAAGAGTG CCTCTCGCTG 
CCGGCCATCG TCGAGCGAAA GAATCTGATC TACGCCCTTC CGACGAGCGG TGGTAAAACC 
CTGGTCGCCG AAATTATCAT GTTCCGGGAG GTACTGCTCC GGAATCGCAG CGTAATGTTC 
ATCGTCCCGT ACGTGTCGTT GGCCCAGGAA AAGCTAGCCG CCCTGTCGCC CTTTGCCGTG 
GCCACGCAGT TCCTCGTGGA GGAATACAGC GGTGGCAAGG GAATGATTCC ACCCCGGAAA 
CGCCGAAGGA AGCAGTCCAT CTTCGTGTGC ACTATCGAGA AGGCACTGGT CTTGCTGGAT 
AGTCTGATCG AGGCCGATCG GGCGAACGAA ATAGGATTGA TTTGCATAGA TGAGTTGCAC 
ATGATCGGTG AACCCAATCG TGGGGTCAAC CTGGAAGTGC TGATCACCAA AGTGCAAGCC 
ATCCAGGCCG GGATTCAGAT CATCGGGATG AGTGCCACGA TCGGAAATCT GGCGGAGATA 
GCCAAGTTCA TGTCGGCGGA CATTTTCAGC CAGGACTTCC GGCCGGTGGA GTTGAAAGAG 
TTTGTGAAAT GTGGTAGCGA TATGTTGGAG ATCAAGCACG GCGCTCGGAC TTTGGAGGAA 
GCATTCGTTA ACAAGCGAAC GGTTAATTAC AAATATTCCG ACACGGATAC CCGAATCGAT 
CCGGACCATA TAATTGGCCT AGTAATGGAA ACTATCCCGA ATGATCCCTG CCTGATCTTT 
TGTCCTACGA AACGTCGATG TGAACAGCTA TGTTCGTTTC TGATTGAGCA CTTGCCGGAT 
CGAGTGGCGG ACTATCGTGC CCCAGAGCGG AAAACATTGA TCGACCTTCT CGAAAATGAC 
GGATCGACGG CTACTTTGTT GCCAGCGGCC TTCCGATTAG GCATTGCCTA CCACCATTCC 
GGACTGACCT TCGACGAACG AAAACTCATT GAGGATGCTT TCCGGGCGAA AGTGCTCTCG 
CTCATCATTT GCACTTCCAC CCTGGCTGCC GGAGTTAATC TACCGGCCAA ACGGGTGATC 
ATACGATCGC CATACATTGC CACCGAGTTC CTGACCCTAA GTCGGTACAA ACAAATGGTT 
GGTCGAGCTG GTCGGGCCGG ATTCGGCAAT GAAGGCGGCG AAAGCATTCT GATCTGTGCC 
CAAAAAGACA TCGCCTTGGT TTGCAACCTT CTTTGCTCTC CGATGGACGA AGCGAACTCC 
TCACTTCATG CGGATAACTT CATACATCTT AAGAACCTAA TACTCAGTGC AATCGGTTTA 
GGCATCTGTT CCACTCGGAA CGACGTCCTA AAGTTTGTCG CCAAAACCCT GCTGGCCATC 
CAGACTGAGC GATTGGGAGT AAACCTCCGG AGTGTCACGG ATGCCGCGAT CACCGCCTTG 
TACAAAGAAA ATGCCATCAA GGCCAAAAGC GACGGTTGTT TGCGAAATCC GGCGAACATG 
ACGGTACAGT TCGATGCGAC CGATCAGACC CAGTACGATT TTGAACCAGG AGGCAACGGT 
TTCCGTGAGC TGGAAGTTCG CCGAGGGCTG GAAGGTTCTT CGCAAGACGG TAAACTGGTG 
AAGATTGTGA AGAAGAACTC CAGGTTGGAG GTCAATCTAC TGGGGAAGGC TTCCATTCGG 
GCTGGATTCC ACATGGAACG AGCAACGCGG GCTTACGATG AATTGAAACG AGTCGGCGAA 
CGACTCTATG TCAATGATGA ACTGCACCTG CTGTGGGTCA TCGTAATGGA GGACGGCGGT 
GAAATTCGAC CGAAGGAAGA CGATTTCATT TCATTCTTCA ACAAGCTTTC GCCGGAAGAG 
ACTAGGATTG CAAAAAGATT TTTCATCAGT GACTACACGA TATCGATGAT CCTCAGCAGA 
CGGTTAGATA AGATAGACAT GAACTTGTTG CGACGCTTCT TCCGAACTCT GATCGTGTAC 
AACCTTTGGA ATCTCATCCC ACCGCAGGAG GTGGCGGTCA GGTTCACCGT GGACGCAGGC 
TCGGTGCAGA CCCTGATGAC CAATGCGGCC GCGACGGCCT CCAGTCTACA GCGGTTCTGC 
GAACAGCTGC CGGAATTTTG GGCATTCGAT TCGCTGCTGA AACGTATGGT GCAACGGCTG 
GCACATTGCT GTACGGCCGA ACTGATCCCT CTGATGGAAC TGCCGGCGGT GAAAATTGGA 
CGGGCACGGC AACTTTATCG AGCCGGATAC ACCTCTTTGT CTTCGATAGC GAAAGCGAAA 
TCAAGTGATC TGGTGTCTAC GGTCCAGCAC ATGAACTACC GGACGGCGAA TCAGTTGATT 
TTATCGGCGA AGGCCAAACT AATGGAGGAA TTTGAGGAGC TCCGGGATCG AATGGATGAC 
TATTTTCATC TCACATCCAT CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL011649 ( XM_001661768.2 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001661818.2 Aedes aegypti helicase POLQ-like (LOC5575094), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe126153
Clone ID Related Accession (Same CDS sequence)	XM_001661768.2
Accession Version	XM_001661768.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	helicase POLQ-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001661768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAATC ACTATCCGTT CGCTCGTCAA CCTCCCCGCC CGAGAATGAG TGGATTGTTC 
AAAGTCCCGA GAGCGCCCCC GGGAGGAGGC CCTCCTACGC TTTCGGCTGG TGGCAGTAGA 
ATTGGTTCCT CTAGCAGCAC TTCCTCGACA AGACCGCGGT TGAATTCCTC CCAGAGCGTT 
TTCTCCAAAA GGCGGCAGGT CTTCGGTGGA GTAACACAAT CAATTTCAGC TTTTCGGGAA 
CCGACACGCA ACGGAATCGT CGGAGAGTCG ACACGCCAAT TGATGGACAT GGACGAAACA 
CTGCTGAATG CGGTCAATTT GGACGTGATT GAAAAGCAGG CGGATACTCC GCCGGTTCCA 
GGGGAGGAAG AGCGGCCAGA AGGTGCAAAC GGTGCAGCAA AAGATAAACA CCGATTTTCG 
TCTCCCGGTA TGACTCCCAG AAGGAAAAAG ATCCGCCGAA ACCCAGGACT GATAGTGTTG 
GATATGACCA AGCCAGAAGT TGACTCAGTA ATTGCCCCAG CACCAAAATC GAAGCCCCCC 
TCGGACGATA CGATGCGAGC CGAAACTTCC CACCGAATGA ACCAAACCCG GACAATTCCG 
GTGATCCCTT CCCAGACGGC GGTCGCAGTG GCAGATAGAC GTCCAACAAT CCCCCCGCCA 
ACAACCATGA TCCCCAGAGA TCCCACCGTG GATCTGTTCG AGTGGGACTC CCAGGCTTTC 
GGCACACAAG TCGAACGGAT TGTGAGCCGA AACGTTGCCT CGAGGAGTTC CCCTCCACGC 
GATCTTGTAG ACGACTACGA TGATTTCCCT TGCTATCAGC AAACCAACGC TAACCAGCCG 
GAGGTTTCCG AGCTGAACGA CAACGACGAC AATCTGAATT GCTCGCAATT GTTCATGGAG 
GAAATCGCGC GGGAACCTCA CCGCCCTTTG ACCCAGATGA GATACTCCCA GCAAAGGGAT 
CCTCACTCGG AACGACCGAA CGCGAACGCC ACCACATATT CGCAGTACAT CCGTAATGAG 
TACACCATGC TGGAGGAGAA CCGTGCCAAC GTGATCGAGG ACGAGGTGGA CATCGCCCTG 
GCCCAACAGT TCGATTCCAG TCAGTACCGG ACGGAGCAGG AAAGCAAATT GAGGATATGT 
GAACGCGAAA TGCTCGACAT GAGCAACATC AACTGGGACG AGAGCGTTGC CATTGCGGGG 
GAGAGGCCAT CGACTTCCAC GTCTACGGCC AAGGCTCGTC TGGATGGGAT CTTTGGGAAG 
ACGGACAGGG CGAACGGAAG GGTCGTGGAA TCGCCGAAGC TGGTGGCCAA AAAGTCGGGG 
CCGCTGGTGT CGTCGCACTT CCAGGATTTG GGCCCATTCT ATGGATTGCC GAACAAGATC 
CGCGATTTGC TTCGGGAGTT TCGCGGGATT ACGGAGATGT ATGATTGGCA GAAAGAGTGC 
CTCTCGCTGC CGGCCATCGT CGAGCGAAAG AATCTGATCT ACGCCCTTCC GACGAGCGGT 
GGTAAAACCC TGGTCGCCGA AATTATCATG TTCCGGGAGG TACTGCTCCG GAATCGCAGC 
GTAATGTTCA TCGTCCCGTA CGTGTCGTTG GCCCAGGAAA AGCTAGCCGC CCTGTCGCCC 
TTTGCCGTGG CCACGCAGTT CCTCGTGGAG GAATACAGCG GTGGCAAGGG AATGATTCCA 
CCCCGGAAAC GCCGAAGGAA GCAGTCCATC TTCGTGTGCA CTATCGAGAA GGCACTGGTC 
TTGCTGGATA GTCTGATCGA GGCCGATCGG GCGAACGAAA TAGGATTGAT TTGCATAGAT 
GAGTTGCACA TGATCGGTGA ACCCAATCGT GGGGTCAACC TGGAAGTGCT GATCACCAAA 
GTGCAAGCCA TCCAGGCCGG GATTCAGATC ATCGGGATGA GTGCCACGAT CGGAAATCTG 
GCGGAGATAG CCAAGTTCAT GTCGGCGGAC ATTTTCAGCC AGGACTTCCG GCCGGTGGAG 
TTGAAAGAGT TTGTGAAATG TGGTAGCGAT ATGTTGGAGA TCAAGCACGG CGCTCGGACT 
TTGGAGGAAG CATTCGTTAA CAAGCGAACG GTTAATTACA AATATTCCGA CACGGATACC 
CGAATCGATC CGGACCATAT AATTGGCCTA GTAATGGAAA CTATCCCGAA TGATCCCTGC 
CTGATCTTTT GTCCTACGAA ACGTCGATGT GAACAGCTAT GTTCGTTTCT GATTGAGCAC 
TTGCCGGATC GAGTGGCGGA CTATCGTGCC CCAGAGCGGA AAACATTGAT CGACCTTCTC 
GAAAATGACG GATCGACGGC TACTTTGTTG CCAGCGGCCT TCCGATTAGG CATTGCCTAC 
CACCATTCCG GACTGACCTT CGACGAACGA AAACTCATTG AGGATGCTTT CCGGGCGAAA 
GTGCTCTCGC TCATCATTTG CACTTCCACC CTGGCTGCCG GAGTTAATCT ACCGGCCAAA 
CGGGTGATCA TACGATCGCC ATACATTGCC ACCGAGTTCC TGACCCTAAG TCGGTACAAA 
CAAATGGTTG GTCGAGCTGG TCGGGCCGGA TTCGGCAATG AAGGCGGCGA AAGCATTCTG 
ATCTGTGCCC AAAAAGACAT CGCCTTGGTT TGCAACCTTC TTTGCTCTCC GATGGACGAA 
GCGAACTCCT CACTTCATGC GGATAACTTC ATACATCTTA AGAACCTAAT ACTCAGTGCA 
ATCGGTTTAG GCATCTGTTC CACTCGGAAC GACGTCCTAA AGTTTGTCGC CAAAACCCTG 
CTGGCCATCC AGACTGAGCG ATTGGGAGTA AACCTCCGGA GTGTCACGGA TGCCGCGATC 
ACCGCCTTGT ACAAAGAAAA TGCCATCAAG GCCAAAAGCG ACGGTTGTTT GCGAAATCCG 
GCGAACATGA CGGTACAGTT CGATGCGACC GATCAGACCC AGTACGATTT TGAACCAGGA 
GGCAACGGTT TCCGTGAGCT GGAAGTTCGC CGAGGGCTGG AAGGTTCTTC GCAAGACGGT 
AAACTGGTGA AGATTGTGAA GAAGAACTCC AGGTTGGAGG TCAATCTACT GGGGAAGGCT 
TCCATTCGGG CTGGATTCCA CATGGAACGA GCAACGCGGG CTTACGATGA ATTGAAACGA 
GTCGGCGAAC GACTCTATGT CAATGATGAA CTGCACCTGC TGTGGGTCAT CGTAATGGAG 
GACGGCGGTG AAATTCGACC GAAGGAAGAC GATTTCATTT CATTCTTCAA CAAGCTTTCG 
CCGGAAGAGA CTAGGATTGC AAAAAGATTT TTCATCAGTG ACTACACGAT ATCGATGATC 
CTCAGCAGAC GGTTAGATAA GATAGACATG AACTTGTTGC GACGCTTCTT CCGAACTCTG 
ATCGTGTACA ACCTTTGGAA TCTCATCCCA CCGCAGGAGG TGGCGGTCAG GTTCACCGTG 
GACGCAGGCT CGGTGCAGAC CCTGATGACC AATGCGGCCG CGACGGCCTC CAGTCTACAG 
CGGTTCTGCG AACAGCTGCC GGAATTTTGG GCATTCGATT CGCTGCTGAA ACGTATGGTG 
CAACGGCTGG CACATTGCTG TACGGCCGAG CTGATCCCTC TGATGGAACT GCCGGCGGTG 
AAAATTGGAC GGGCACGGCA ACTTTATCGA GCCGGATACA CCTCTTTGTC TTCGATAGCG 
AAAGCGAAAT CAAGTGATCT GGTGTCTACG GTCCAGCACA TGAACTACCG GACGGCGAAT 
CAGTTGATTT TATCGGCGAA GGCCAAACTA ATGGAGGAAT TTGAGGAGCT CCGGGATCGA 
ATGGATGACT ATTTTCATCT CACATCCATC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001661818.2
CDS	144..3959
Translation	MPNHYPFARQPPRPRMSGLFKVPRAPPGGGPPTLSAGGSRIGSSSSTSSTRPRLNSSQSVFSKRRQVFGGVTQSISAFREPTRNGIVGESTRQLMDMDETLLNAVNLDVIEKQADTPPVPGEEERPEGANGAAKDKHRFSSPGMTPRRKKIRRNPGLIVLDMTKPEVDSVIAPAPKSKPPSDDTMRAETSHRMNQTRTIPVIPSQTAVAVADRRPTIPPPTTMIPRDPTVDLFEWDSQAFGTQVERIVSRNVASRSSPPRDLVDDYDDFPCYQQTNANQPEVSELNDNDDNLNCSQLFMEEIAREPHRPLTQMRYSQQRDPHSERPNANATTYSQYIRNEYTMLEENRANVIEDEVDIALAQQFDSSQYRTEQESKLRICEREMLDMSNINWDESVAIAGERPSTSTSTAKARLDGIFGKTDRANGRVVESPKLVAKKSGPLVSSHFQDLGPFYGLPNKIRDLLREFRGITEMYDWQKECLSLPAIVERKNLIYALPTSGGKTLVAEIIMFREVLLRNRSVMFIVPYVSLAQEKLAALSPFAVATQFLVEEYSGGKGMIPPRKRRRKQSIFVCTIEKALVLLDSLIEADRANEIGLICIDELHMIGEPNRGVNLEVLITKVQAIQAGIQIIGMSATIGNLAEIAKFMSADIFSQDFRPVELKEFVKCGSDMLEIKHGARTLEEAFVNKRTVNYKYSDTDTRIDPDHIIGLVMETIPNDPCLIFCPTKRRCEQLCSFLIEHLPDRVADYRAPERKTLIDLLENDGSTATLLPAAFRLGIAYHHSGLTFDERKLIEDAFRAKVLSLIICTSTLAAGVNLPAKRVIIRSPYIATEFLTLSRYKQMVGRAGRAGFGNEGGESILICAQKDIALVCNLLCSPMDEANSSLHADNFIHLKNLILSAIGLGICSTRNDVLKFVAKTLLAIQTERLGVNLRSVTDAAITALYKENAIKAKSDGCLRNPANMTVQFDATDQTQYDFEPGGNGFRELEVRRGLEGSSQDGKLVKIVKKNSRLEVNLLGKASIRAGFHMERATRAYDELKRVGERLYVNDELHLLWVIVMEDGGEIRPKEDDFISFFNKLSPEETRIAKRFFISDYTISMILSRRLDKIDMNLLRRFFRTLIVYNLWNLIPPQEVAVRFTVDAGSVQTLMTNAAATASSLQRFCEQLPEFWAFDSLLKRMVQRLAHCCTAELIPLMELPAVKIGRARQLYRAGYTSLSSIAKAKSSDLVSTVQHMNYRTANQLILSAKAKLMEEFEELRDRMDDYFHLTSIS

Target ORF information:

RefSeq Version	XM_001661768.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti helicase POLQ-like (LOC5575094), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001661768.2

ORF Insert Sequence:

ATGCCCAATC ACTATCCGTT CGCTCGTCAA CCTCCCCGCC CGAGAATGAG TGGATTGTTC 
AAAGTCCCGA GAGCGCCCCC GGGAGGAGGC CCTCCTACGC TTTCGGCTGG TGGCAGTAGA 
ATTGGTTCCT CTAGCAGCAC TTCCTCGACA AGACCGCGGT TGAATTCCTC CCAGAGCGTT 
TTCTCCAAAA GGCGGCAGGT CTTCGGTGGA GTAACACAAT CAATTTCAGC TTTTCGGGAA 
CCGACACGCA ACGGAATCGT CGGAGAGTCG ACACGCCAAT TGATGGACAT GGACGAAACA 
CTGCTGAATG CGGTCAATTT GGACGTGATT GAAAAGCAGG CGGATACTCC GCCGGTTCCA 
GGGGAGGAAG AGCGGCCAGA AGGTGCAAAC GGTGCAGCAA AAGATAAACA CCGATTTTCG 
TCTCCCGGTA TGACTCCCAG AAGGAAAAAG ATCCGCCGAA ACCCAGGACT GATAGTGTTG 
GATATGACCA AGCCAGAAGT TGACTCAGTA ATTGCCCCAG CACCAAAATC GAAGCCCCCC 
TCGGACGATA CGATGCGAGC CGAAACTTCC CACCGAATGA ACCAAACCCG GACAATTCCG 
GTGATCCCTT CCCAGACGGC GGTCGCAGTG GCAGATAGAC GTCCAACAAT CCCCCCGCCA 
ACAACCATGA TCCCCAGAGA TCCCACCGTG GATCTGTTCG AGTGGGACTC CCAGGCTTTC 
GGCACACAAG TCGAACGGAT TGTGAGCCGA AACGTTGCCT CGAGGAGTTC CCCTCCACGC 
GATCTTGTAG ACGACTACGA TGATTTCCCT TGCTATCAGC AAACCAACGC TAACCAGCCG 
GAGGTTTCCG AGCTGAACGA CAACGACGAC AATCTGAATT GCTCGCAATT GTTCATGGAG 
GAAATCGCGC GGGAACCTCA CCGCCCTTTG ACCCAGATGA GATACTCCCA GCAAAGGGAT 
CCTCACTCGG AACGACCGAA CGCGAACGCC ACCACATATT CGCAGTACAT CCGTAATGAG 
TACACCATGC TGGAGGAGAA CCGTGCCAAC GTGATCGAGG ACGAGGTGGA CATCGCCCTG 
GCCCAACAGT TCGATTCCAG TCAGTACCGG ACGGAGCAGG AAAGCAAATT GAGGATATGT 
GAACGCGAAA TGCTCGACAT GAGCAACATC AACTGGGACG AGAGCGTTGC CATTGCGGGG 
GAGAGGCCAT CGACTTCCAC GTCTACGGCC AAGGCTCGTC TGGATGGGAT CTTTGGGAAG 
ACGGACAGGG CGAACGGAAG GGTCGTGGAA TCGCCGAAGC TGGTGGCCAA AAAGTCGGGG 
CCGCTGGTGT CGTCGCACTT CCAGGATTTG GGCCCATTCT ATGGATTGCC GAACAAGATC 
CGCGATTTGC TTCGGGAGTT TCGCGGGATT ACGGAGATGT ATGATTGGCA GAAAGAGTGC 
CTCTCGCTGC CGGCCATCGT CGAGCGAAAG AATCTGATCT ACGCCCTTCC GACGAGCGGT 
GGTAAAACCC TGGTCGCCGA AATTATCATG TTCCGGGAGG TACTGCTCCG GAATCGCAGC 
GTAATGTTCA TCGTCCCGTA CGTGTCGTTG GCCCAGGAAA AGCTAGCCGC CCTGTCGCCC 
TTTGCCGTGG CCACGCAGTT CCTCGTGGAG GAATACAGCG GTGGCAAGGG AATGATTCCA 
CCCCGGAAAC GCCGAAGGAA GCAGTCCATC TTCGTGTGCA CTATCGAGAA GGCACTGGTC 
TTGCTGGATA GTCTGATCGA GGCCGATCGG GCGAACGAAA TAGGATTGAT TTGCATAGAT 
GAGTTGCACA TGATCGGTGA ACCCAATCGT GGGGTCAACC TGGAAGTGCT GATCACCAAA 
GTGCAAGCCA TCCAGGCCGG GATTCAGATC ATCGGGATGA GTGCCACGAT CGGAAATCTG 
GCGGAGATAG CCAAGTTCAT GTCGGCGGAC ATTTTCAGCC AGGACTTCCG GCCGGTGGAG 
TTGAAAGAGT TTGTGAAATG TGGTAGCGAT ATGTTGGAGA TCAAGCACGG CGCTCGGACT 
TTGGAGGAAG CATTCGTTAA CAAGCGAACG GTTAATTACA AATATTCCGA CACGGATACC 
CGAATCGATC CGGACCATAT AATTGGCCTA GTAATGGAAA CTATCCCGAA TGATCCCTGC 
CTGATCTTTT GTCCTACGAA ACGTCGATGT GAACAGCTAT GTTCGTTTCT GATTGAGCAC 
TTGCCGGATC GAGTGGCGGA CTATCGTGCC CCAGAGCGGA AAACATTGAT CGACCTTCTC 
GAAAATGACG GATCGACGGC TACTTTGTTG CCAGCGGCCT TCCGATTAGG CATTGCCTAC 
CACCATTCCG GACTGACCTT CGACGAACGA AAACTCATTG AGGATGCTTT CCGGGCGAAA 
GTGCTCTCGC TCATCATTTG CACTTCCACC CTGGCTGCCG GAGTTAATCT ACCGGCCAAA 
CGGGTGATCA TACGATCGCC ATACATTGCC ACCGAGTTCC TGACCCTAAG TCGGTACAAA 
CAAATGGTTG GTCGAGCTGG TCGGGCCGGA TTCGGCAATG AAGGCGGCGA AAGCATTCTG 
ATCTGTGCCC AAAAAGACAT CGCCTTGGTT TGCAACCTTC TTTGCTCTCC GATGGACGAA 
GCGAACTCCT CACTTCATGC GGATAACTTC ATACATCTTA AGAACCTAAT ACTCAGTGCA 
ATCGGTTTAG GCATCTGTTC CACTCGGAAC GACGTCCTAA AGTTTGTCGC CAAAACCCTG 
CTGGCCATCC AGACTGAGCG ATTGGGAGTA AACCTCCGGA GTGTCACGGA TGCCGCGATC 
ACCGCCTTGT ACAAAGAAAA TGCCATCAAG GCCAAAAGCG ACGGTTGTTT GCGAAATCCG 
GCGAACATGA CGGTACAGTT CGATGCGACC GATCAGACCC AGTACGATTT TGAACCAGGA 
GGCAACGGTT TCCGTGAGCT GGAAGTTCGC CGAGGGCTGG AAGGTTCTTC GCAAGACGGT 
AAACTGGTGA AGATTGTGAA GAAGAACTCC AGGTTGGAGG TCAATCTACT GGGGAAGGCT 
TCCATTCGGG CTGGATTCCA CATGGAACGA GCAACGCGGG CTTACGATGA ATTGAAACGA 
GTCGGCGAAC GACTCTATGT CAATGATGAA CTGCACCTGC TGTGGGTCAT CGTAATGGAG 
GACGGCGGTG AAATTCGACC GAAGGAAGAC GATTTCATTT CATTCTTCAA CAAGCTTTCG 
CCGGAAGAGA CTAGGATTGC AAAAAGATTT TTCATCAGTG ACTACACGAT ATCGATGATC 
CTCAGCAGAC GGTTAGATAA GATAGACATG AACTTGTTGC GACGCTTCTT CCGAACTCTG 
ATCGTGTACA ACCTTTGGAA TCTCATCCCA CCGCAGGAGG TGGCGGTCAG GTTCACCGTG 
GACGCAGGCT CGGTGCAGAC CCTGATGACC AATGCGGCCG CGACGGCCTC CAGTCTACAG 
CGGTTCTGCG AACAGCTGCC GGAATTTTGG GCATTCGATT CGCTGCTGAA ACGTATGGTG 
CAACGGCTGG CACATTGCTG TACGGCCGAG CTGATCCCTC TGATGGAACT GCCGGCGGTG 
AAAATTGGAC GGGCACGGCA ACTTTATCGA GCCGGATACA CCTCTTTGTC TTCGATAGCG 
AAAGCGAAAT CAAGTGATCT GGTGTCTACG GTCCAGCACA TGAACTACCG GACGGCGAAT 
CAGTTGATTT TATCGGCGAA GGCCAAACTA ATGGAGGAAT TTGAGGAGCT CCGGGATCGA 
ATGGATGACT ATTTTCATCT CACATCCATC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL011649 ( XM_021850281.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021705973.1 Aedes aegypti helicase POLQ-like (LOC5575094), transcript variant X2, mRNA.

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CloneID	OAe126154
Clone ID Related Accession (Same CDS sequence)	XM_021850281.1
Accession Version	XM_021850281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	helicase POLQ-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAATC ACTATCCGTT CGCTCGTCAA CCTCCCCGCC CGAGAATGAG TGGATTGTTC 
AAAGTCCCGA GAGCGCCCCC GGGAGGAGGC CCTCCTACGC TTTCGGCTGG TGGCAGTAGA 
ATTGGTTCCT CTAGCAGCAC TTCCTCGACA AGACCGCGGT TGAATTCCTC CCAGAGCGTT 
TTCTCCAAAA GGCGGCAGGT CTTCGGTGGA GTAACACAAT CAATTTCAGC TTTTCGGGAA 
CCGACACGCA ACGGAATCGT CGGAGAGTCG ACACGCCAAT TGATGGACAT GGACGAAACA 
CTGCTGAATG CGGTCAATTT GGACGTGATT GAAAAGCAGG CGGATACTCC GCCGGTTCCA 
GGGGAGGAAG AGCGGCCAGA AGGTGCAAAC GGTGCAGCAA AAGATAAACA CCGATTTTCG 
TCTCCCGGTA TGACTCCCAG AAGGAAAAAG ATCCGCCGAA ACCCAGGACT GATAGTGTTG 
GATATGACCA AGCCAGAAGT TGACTCAGTA ATTGCCCCAG CACCAAAATC GAAGCCCCCC 
TCGGACGATA CGATGCGAGC CGAAACTTCC CACCGAATGA ACCAAACCCG GACAATTCCG 
GTGATCCCTT CCCAGACGGC GGTCGCAGTG GCAGATAGAC GTCCAACAAT CCCCCCGCCA 
ACAACCATGA TCCCCAGAGA TCCCACCGTG GATCTGTTCG AGTGGGACTC CCAGGCTTTC 
GGCACACAAG TCGAACGGAT TGTGAGCCGA AACGTTGCCT CGAGGAGTTC CCCTCCACGC 
GATCTTGTAG ACGACTACGA TGATTTCCCT TGCTATCAGC AAACCAACGC TAACCAGCCG 
GAGGTTTCCG AGCTGAACGA CAACGACGAC AATCTGAATT GCTCGCAATT GTTCATGGAG 
GAAATCGCGC GGGAACCTCA CCGCCCTTTG ACCCAGATGA GATACTCCCA GCAAAGGGAT 
CCTCACTCGG AACGACCGAA CGCGAACGCC ACCACATATT CGCAGTACAT CCGTAATGAG 
TACACCATGC TGGAGGAGAA CCGTGCCAAC GTGATCGAGG ACGAGGTGGA CATCGCCCTG 
GCCCAACAGT TCGATTCCAG TCAGTACCGG ACGGAGCAGG AAAGCAAATT GAGGATATGT 
GAACGCGAAA TGCTCGACAT GAGCAACATC AACTGGGACG AGAGCGTTGC CATTGCGGGG 
GAGAGGCCAT CGACTTCCAC GTCTACGGCC AAGGCTCGTC TGGATGGGAT CTTTGGGAAG 
ACGGACAGGG CGAACGGAAG GGTCGTGGAA TCGCCGAAGC TGGTGGCCAA AAAGTCGGGG 
CCGCTGGTGT CGTCGCACTT CCAGGATTTG GGCCCATTCT ATGGATTGCC GAACAAGATC 
CGCGATTTGC TTCGGGAGTT TCGCGGGATT ACGGAGATGT ATGATTGGCA GAAAGAGTGC 
CTCTCGCTGC CGGCCATCGT CGAGCGAAAG AATCTGATCT ACGCCCTTCC GACGAGCGGT 
GGTAAAACCC TGGTCGCCGA AATTATCATG TTCCGGGAGG TACTGCTCCG GAATCGCAGC 
GTAATGTTCA TCGTCCCGTA CGTGTCGTTG GCCCAGGAAA AGCTAGCCGC CCTGTCGCCC 
TTTGCCGTGG CCACGCAGTT CCTCGTGGAG GAATACAGCG GTGGCAAGGG AATGATTCCA 
CCCCGGAAAC GCCGAAGGAA GCAGTCCATC TTCGTGTGCA CTATCGAGAA GGCACTGGTC 
TTGCTGGATA GTCTGATCGA GGCCGATCGG GCGAACGAAA TAGGATTGAT TTGCATAGAT 
GAGTTGCACA TGATCGGTGA ACCCAATCGT GGGGTCAACC TGGAAGTGCT GATCACCAAA 
GTGCAAGCCA TCCAGGCCGG GATTCAGATC ATCGGGATGA GTGCCACGAT CGGAAATCTG 
GCGGAGATAG CCAAGTTCAT GTCGGCGGAC ATTTTCAGCC AGGACTTCCG GCCGGTGGAG 
TTGAAAGAGT TTGTGAAATG TGGTAGCGAT ATGTTGGAGA TCAAGCACGG CGCTCGGACT 
TTGGAGGAAG CATTCGTTAA CAAGCGAACG GTTAATTACA AATATTCCGA CACGGATACC 
CGAATCGATC CGGACCATAT AATTGGCCTA GTAATGGAAA CTATCCCGAA TGATCCCTGC 
CTGATCTTTT GTCCTACGAA ACGTCGATGT GAACAGCTAT GTTCGTTTCT GATTGAGCAC 
TTGCCGGATC GAGTGGCGGA CTATCGTGCC CCAGAGCGGA AAACATTGAT CGACCTTCTC 
GAAAATGACG GATCGACGGC TACTTTGTTG CCAGCGGCCT TCCGATTAGG CATTGCCTAC 
CACCATTCCG GACTGACCTT CGACGAACGA AAACTCATTG AGGATGCTTT CCGGGCGAAA 
GTGCTCTCGC TCATCATTTG CACTTCCACC CTGGCTGCCG GAGTTAATCT ACCGGCCAAA 
CGGGTGATCA TACGATCGCC ATACATTGCC ACCGAGTTCC TGACCCTAAG TCGGTACAAA 
CAAATGGTTG GTCGAGCTGG TCGGGCCGGA TTCGGCAATG AAGGCGGCGA AAGCATTCTG 
ATCTGTGCCC AAAAAGACAT CGCCTTGGTT TGCAACCTTC TTTGCTCTCC GATGGACGAA 
GCGAACTCCT CACTTCATGC GGATAACTTC ATACATCTTA AGAACCTAAT ACTCAGTGCA 
ATCGGTTTAG GCATCTGTTC CACTCGGAAC GACGTCCTAA AGTTTGTCGC CAAAACCCTG 
CTGGCCATCC AGACTGAGCG ATTGGGAGTA AACCTCCGGA GTGTCACGGA TGCCGCGATC 
ACCGCCTTGT ACAAAGAAAA TGCCATCAAG GCCAAAAGCG ACGGTTGTTT GCGAAATCCG 
GCGAACATGA CGGTACAGTT CGATGCGACC GATCAGACCC AGTACGATTT TGAACCAGGA 
GGCAACGGTT TCCGTGAGCT GGAAGTTCGC CGAGGGCTGG AAGGTTCTTC GCAAGACGGT 
AAACTGGTGA AGATTGTGAA GAAGAACTCC AGGTTGGAGG TCAATCTACT GGGGAAGGCT 
TCCATTCGGG CTGGATTCCA CATGGAACGA GCAACGCGGG CTTACGATGA ATTGAAACGA 
GTCGGCGAAC GACTCTATGT CAATGATGAA CTGCACCTGC TGTGGGTCAT CGTAATGGAG 
GACGGCGGTG AAATTCGACC GAAGGAAGAC GATTTCATTT CATTCTTCAA CAAGCTTTCG 
CCGGAAGAGA CTAGGATTGC AAAAAGATTT TTCATCAGTG ACTACACGAT ATCGATGATC 
CTCAGCAGAC GGTTAGATAA GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021705973.1
CDS	145..3471
Translation	MPNHYPFARQPPRPRMSGLFKVPRAPPGGGPPTLSAGGSRIGSSSSTSSTRPRLNSSQSVFSKRRQVFGGVTQSISAFREPTRNGIVGESTRQLMDMDETLLNAVNLDVIEKQADTPPVPGEEERPEGANGAAKDKHRFSSPGMTPRRKKIRRNPGLIVLDMTKPEVDSVIAPAPKSKPPSDDTMRAETSHRMNQTRTIPVIPSQTAVAVADRRPTIPPPTTMIPRDPTVDLFEWDSQAFGTQVERIVSRNVASRSSPPRDLVDDYDDFPCYQQTNANQPEVSELNDNDDNLNCSQLFMEEIAREPHRPLTQMRYSQQRDPHSERPNANATTYSQYIRNEYTMLEENRANVIEDEVDIALAQQFDSSQYRTEQESKLRICEREMLDMSNINWDESVAIAGERPSTSTSTAKARLDGIFGKTDRANGRVVESPKLVAKKSGPLVSSHFQDLGPFYGLPNKIRDLLREFRGITEMYDWQKECLSLPAIVERKNLIYALPTSGGKTLVAEIIMFREVLLRNRSVMFIVPYVSLAQEKLAALSPFAVATQFLVEEYSGGKGMIPPRKRRRKQSIFVCTIEKALVLLDSLIEADRANEIGLICIDELHMIGEPNRGVNLEVLITKVQAIQAGIQIIGMSATIGNLAEIAKFMSADIFSQDFRPVELKEFVKCGSDMLEIKHGARTLEEAFVNKRTVNYKYSDTDTRIDPDHIIGLVMETIPNDPCLIFCPTKRRCEQLCSFLIEHLPDRVADYRAPERKTLIDLLENDGSTATLLPAAFRLGIAYHHSGLTFDERKLIEDAFRAKVLSLIICTSTLAAGVNLPAKRVIIRSPYIATEFLTLSRYKQMVGRAGRAGFGNEGGESILICAQKDIALVCNLLCSPMDEANSSLHADNFIHLKNLILSAIGLGICSTRNDVLKFVAKTLLAIQTERLGVNLRSVTDAAITALYKENAIKAKSDGCLRNPANMTVQFDATDQTQYDFEPGGNGFRELEVRRGLEGSSQDGKLVKIVKKNSRLEVNLLGKASIRAGFHMERATRAYDELKRVGERLYVNDELHLLWVIVMEDGGEIRPKEDDFISFFNKLSPEETRIAKRFFISDYTISMILSRRLDKT

Target ORF information:

RefSeq Version	XM_021850281.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti helicase POLQ-like (LOC5575094), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021850281.1

ORF Insert Sequence:

ATGCCCAATC ACTATCCGTT CGCTCGTCAA CCTCCCCGCC CGAGAATGAG TGGATTGTTC 
AAAGTCCCGA GAGCGCCCCC GGGAGGAGGC CCTCCTACGC TTTCGGCTGG TGGCAGTAGA 
ATTGGTTCCT CTAGCAGCAC TTCCTCGACA AGACCGCGGT TGAATTCCTC CCAGAGCGTT 
TTCTCCAAAA GGCGGCAGGT CTTCGGTGGA GTAACACAAT CAATTTCAGC TTTTCGGGAA 
CCGACACGCA ACGGAATCGT CGGAGAGTCG ACACGCCAAT TGATGGACAT GGACGAAACA 
CTGCTGAATG CGGTCAATTT GGACGTGATT GAAAAGCAGG CGGATACTCC GCCGGTTCCA 
GGGGAGGAAG AGCGGCCAGA AGGTGCAAAC GGTGCAGCAA AAGATAAACA CCGATTTTCG 
TCTCCCGGTA TGACTCCCAG AAGGAAAAAG ATCCGCCGAA ACCCAGGACT GATAGTGTTG 
GATATGACCA AGCCAGAAGT TGACTCAGTA ATTGCCCCAG CACCAAAATC GAAGCCCCCC 
TCGGACGATA CGATGCGAGC CGAAACTTCC CACCGAATGA ACCAAACCCG GACAATTCCG 
GTGATCCCTT CCCAGACGGC GGTCGCAGTG GCAGATAGAC GTCCAACAAT CCCCCCGCCA 
ACAACCATGA TCCCCAGAGA TCCCACCGTG GATCTGTTCG AGTGGGACTC CCAGGCTTTC 
GGCACACAAG TCGAACGGAT TGTGAGCCGA AACGTTGCCT CGAGGAGTTC CCCTCCACGC 
GATCTTGTAG ACGACTACGA TGATTTCCCT TGCTATCAGC AAACCAACGC TAACCAGCCG 
GAGGTTTCCG AGCTGAACGA CAACGACGAC AATCTGAATT GCTCGCAATT GTTCATGGAG 
GAAATCGCGC GGGAACCTCA CCGCCCTTTG ACCCAGATGA GATACTCCCA GCAAAGGGAT 
CCTCACTCGG AACGACCGAA CGCGAACGCC ACCACATATT CGCAGTACAT CCGTAATGAG 
TACACCATGC TGGAGGAGAA CCGTGCCAAC GTGATCGAGG ACGAGGTGGA CATCGCCCTG 
GCCCAACAGT TCGATTCCAG TCAGTACCGG ACGGAGCAGG AAAGCAAATT GAGGATATGT 
GAACGCGAAA TGCTCGACAT GAGCAACATC AACTGGGACG AGAGCGTTGC CATTGCGGGG 
GAGAGGCCAT CGACTTCCAC GTCTACGGCC AAGGCTCGTC TGGATGGGAT CTTTGGGAAG 
ACGGACAGGG CGAACGGAAG GGTCGTGGAA TCGCCGAAGC TGGTGGCCAA AAAGTCGGGG 
CCGCTGGTGT CGTCGCACTT CCAGGATTTG GGCCCATTCT ATGGATTGCC GAACAAGATC 
CGCGATTTGC TTCGGGAGTT TCGCGGGATT ACGGAGATGT ATGATTGGCA GAAAGAGTGC 
CTCTCGCTGC CGGCCATCGT CGAGCGAAAG AATCTGATCT ACGCCCTTCC GACGAGCGGT 
GGTAAAACCC TGGTCGCCGA AATTATCATG TTCCGGGAGG TACTGCTCCG GAATCGCAGC 
GTAATGTTCA TCGTCCCGTA CGTGTCGTTG GCCCAGGAAA AGCTAGCCGC CCTGTCGCCC 
TTTGCCGTGG CCACGCAGTT CCTCGTGGAG GAATACAGCG GTGGCAAGGG AATGATTCCA 
CCCCGGAAAC GCCGAAGGAA GCAGTCCATC TTCGTGTGCA CTATCGAGAA GGCACTGGTC 
TTGCTGGATA GTCTGATCGA GGCCGATCGG GCGAACGAAA TAGGATTGAT TTGCATAGAT 
GAGTTGCACA TGATCGGTGA ACCCAATCGT GGGGTCAACC TGGAAGTGCT GATCACCAAA 
GTGCAAGCCA TCCAGGCCGG GATTCAGATC ATCGGGATGA GTGCCACGAT CGGAAATCTG 
GCGGAGATAG CCAAGTTCAT GTCGGCGGAC ATTTTCAGCC AGGACTTCCG GCCGGTGGAG 
TTGAAAGAGT TTGTGAAATG TGGTAGCGAT ATGTTGGAGA TCAAGCACGG CGCTCGGACT 
TTGGAGGAAG CATTCGTTAA CAAGCGAACG GTTAATTACA AATATTCCGA CACGGATACC 
CGAATCGATC CGGACCATAT AATTGGCCTA GTAATGGAAA CTATCCCGAA TGATCCCTGC 
CTGATCTTTT GTCCTACGAA ACGTCGATGT GAACAGCTAT GTTCGTTTCT GATTGAGCAC 
TTGCCGGATC GAGTGGCGGA CTATCGTGCC CCAGAGCGGA AAACATTGAT CGACCTTCTC 
GAAAATGACG GATCGACGGC TACTTTGTTG CCAGCGGCCT TCCGATTAGG CATTGCCTAC 
CACCATTCCG GACTGACCTT CGACGAACGA AAACTCATTG AGGATGCTTT CCGGGCGAAA 
GTGCTCTCGC TCATCATTTG CACTTCCACC CTGGCTGCCG GAGTTAATCT ACCGGCCAAA 
CGGGTGATCA TACGATCGCC ATACATTGCC ACCGAGTTCC TGACCCTAAG TCGGTACAAA 
CAAATGGTTG GTCGAGCTGG TCGGGCCGGA TTCGGCAATG AAGGCGGCGA AAGCATTCTG 
ATCTGTGCCC AAAAAGACAT CGCCTTGGTT TGCAACCTTC TTTGCTCTCC GATGGACGAA 
GCGAACTCCT CACTTCATGC GGATAACTTC ATACATCTTA AGAACCTAAT ACTCAGTGCA 
ATCGGTTTAG GCATCTGTTC CACTCGGAAC GACGTCCTAA AGTTTGTCGC CAAAACCCTG 
CTGGCCATCC AGACTGAGCG ATTGGGAGTA AACCTCCGGA GTGTCACGGA TGCCGCGATC 
ACCGCCTTGT ACAAAGAAAA TGCCATCAAG GCCAAAAGCG ACGGTTGTTT GCGAAATCCG 
GCGAACATGA CGGTACAGTT CGATGCGACC GATCAGACCC AGTACGATTT TGAACCAGGA 
GGCAACGGTT TCCGTGAGCT GGAAGTTCGC CGAGGGCTGG AAGGTTCTTC GCAAGACGGT 
AAACTGGTGA AGATTGTGAA GAAGAACTCC AGGTTGGAGG TCAATCTACT GGGGAAGGCT 
TCCATTCGGG CTGGATTCCA CATGGAACGA GCAACGCGGG CTTACGATGA ATTGAAACGA 
GTCGGCGAAC GACTCTATGT CAATGATGAA CTGCACCTGC TGTGGGTCAT CGTAATGGAG 
GACGGCGGTG AAATTCGACC GAAGGAAGAC GATTTCATTT CATTCTTCAA CAAGCTTTCG 
CCGGAAGAGA CTAGGATTGC AAAAAGATTT TTCATCAGTG ACTACACGAT ATCGATGATC 
CTCAGCAGAC GGTTAGATAA GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
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AAEL_AAEL011649 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

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