LOC5572509 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5572509
Entrez Gene ID	5572509
Full Name	glycogen debranching enzyme
Synonyms	AAEL009838
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001660397.2	XP_001660447.1	glycogen debranching enzyme isoform X1
XM_001660397.1	XP_001660447.1	AAEL009838-PA
XM_021853942.1	XP_021709634.1	glycogen debranching enzyme isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5572509 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5572509 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe07815	XM_001660397.2 Latest version!	Aedes aegypti glycogen debranching enzyme (LOC5572509), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe128981	XM_021853942.1 Latest version!	Aedes aegypti glycogen debranching enzyme (LOC5572509), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OAe07815	XM_001660397.1	Aedes aegypti AAEL009838-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5572509 ( XM_001660397.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001660447.1 Aedes aegypti glycogen debranching enzyme (LOC5572509), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe07815
Clone ID Related Accession (Same CDS sequence)	XM_001660397.2 , XM_001660397.1
Accession Version	XM_001660397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001660397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAAA AGATGGGATC CTTTCTGTCT TATGAGAAAC TGCCGACAAT CTTCGAGGTG 
AAAAAGATGG CCGATCAGAC CGGTTCGATA AAGCTGGCGA TCCACCACAA TGACCAGGGC 
AAAGGCTCGC TCTACCGTCT GAAGAAGAAC TCCATGGTGC ACGTCCATCC GGGACCGTCT 
CTGCTGGGCC GCCGGGTGGC CATCTTCTGC AACTATCCGG CCGATGGTGC TGATTTCGAC 
CGCAACCGGT ACACCCAACT GAAGTGGTTC TCGCCAACGG GAACCCAGCT CGAGTCGGAT 
GGCTCCAAGT ATCTCTCGAT CAAGGATGTT GACTACTATT GCAATGTTCA CCTGACCCGC 
TCCGGGGCGT ACCACTTCTA CATTCTGTAC GAGGGTGAGG AAGAACAGCA GGCTGGACTG 
TATGTGCAAA TCGAGCCTTC TCTAACCATT GGGAAGGGTC CCTCAGCAGC CCAACTGCCC 
CTGGAGGCCA TCCGTTGTCA GACGGTCATC GCTAAGTTGA TGGGTCCTCT TGAAAGCTGG 
GAAGCCAAGT TGCAGGTCAC CAAGGAGAGC GGCTACAATA TGGTTCATTT CACACCGATC 
CAAGAGCTGG GCGGCTCGAG ATCTGCCTAT TCTCTCAAGA ATCAAAATAA AGTGAACCCG 
GAGTTCCAGA ATGGTGATCA ACCCGTTGAG TTCGAAGATG TGAATAAGGT TATCAAGAAA 
ATGCGCGAAG ATTGGGGTAT CGCGTCGATT TGCGATATCG TGCTGAATCA CACTGCGAAC 
GAGTCGGTTT GGATTCGGGA ATATCCGGAG TGCAGCTACA ATTGCTTCAC CTGCCCACAT 
TTGAGGCCGG CCTTCCTGTT GGATGCTTTA TTGGCTCGTG TGGGCGAAGA CGTGGGCGCT 
GGACTACTAG AGCATGTCGG TGTGCCAAAA GTGGTGGAGA CGAATGATCA TCTGCAGGCC 
ATCCATTGGC AGATCCACTC GAACTATCTG CCACAGATTA AGCTGTACGA GTTGTATCAG 
TGTGATGTAG ATAAGTATGT CACCAGGTTT AATGACGAGG CGTCGAAGAA TGGTCCGTCT 
GCGGCAAAAG AAGTACCGGA GGGTGACCTC AAGCTCAAGC ATGATCAGGA GTATAGACGG 
CTGGGAGCAC AAATCGATTG GGACCGGGCC ATGAAGACCT ACAATGTATT CCGCCAGGAC 
TGTTTCGACG AGGACAGTCG GAAGCGCAAA TGTGCAGAAG CATTCCGTTC GAAGTTGCAA 
TGGCTGAACG AGGAAGTTCG TAAGGAGATT CAGGCGCATT TGGATTATGC TATTGAGAAC 
TGCCTGGCTG GAGCTCGGTA TGAGCGAGTG CAAGCTGATG GACCTCAAAT CAAGGGAATT 
TCGGTGAAGT ATCCGTTGTT CATGAAGTAC TTCACTCAGT ACGGAATCCA GGGTAAATCG 
TTGAAGGATA TTGAAGCGAT GATGTACGGA GGAGCTGGTA AGTTGTTCAT GGCTCACAAT 
GGATGGGTGA TTAACAGTGA TCCGCTGAAG GACTTTGCTC GGCCTCAGGA GGGGACGGGT 
AATGTCTATT TGAGGAGGGA ATTGATTGCT TGGGGTGACA GCGTAAAGTT AAGGTACGGC 
GACAAACCGG AAGATAGCCC GTATTTGTGG AAGCACATGA AGGAGTATGT CGACACTACT 
GCGAAGATCT TCGACGGAGT TCGATTGGAC AACTGCCATT CGACGCCGCT GCATGTTGCG 
GAATATCTGC TGGATTCGGC GCGGAAGGTC AACCCGGAAT TGTACGTCGT GGCGGAACTG 
TTTACCAACT CTGATTACAC GGATAACGTT TTCGTCAATC GGTTGGGCAT TACTTCATTG 
ATTCGAGAGG CTTTGTCGGC ATGGGATTCC CACGAGGAAG GTCGACTCGT GTACCGATAT 
GGAGGTGATC CGGTGGGGGC GTTCTTCCCA TACCCGAAAC GTATTCTAGC GCCGAGCATA 
GCCCACGCAC TGTTCATGGA CCTGACGCAC GATAACCCAT CGCCAGTGGA GAAGCGATCG 
ATCTTCGATT TGATTCCGTC TGCTGGTTTG GTTTCTATGG CGTGCTGTGC GACGGGAAGC 
AATCGAGGGT ACGACGAGCT GGTTCCGCAT CACATTCACG TGGTCGACGA AGAACGTCAG 
TACCAGGAGT GGGGAAAACA CGTCGACAAA TCGACCGGCA TCGTGGCGGT TAAGGAAGCT 
TTCAATCTTT TGCACGGTGG TCTTGCGACG CGTAATTTCG ACCAGCTGTT CGTTGACCAA 
ATGCATCCGG ATATTGTGGC CGTCACCCGT CACTCTACGC TGAACCGCGA AACGGTTGTA 
TTGGTGGCGC ATACCGCGTT TAATTACCCC AGTCCTTGGG CTGGTCCGAC GGGAGTTCGC 
CCGCTGCGTG TCGAGGGCGA TTTGGTGGAG ATTATCTTCG AAGTACAGAT CTATAAGAAA 
ACTGGTCAAA CCTTCGATCA ACCCACGGAA TTTAAGAAAG ATGAACACTA CATCAACGGA 
GTCCAAGAAT ATGTGGTTGA TCTGCGCAAG AATTTAAAAC TGGATGAGTC GGATATCTTT 
GAGAAGACAC CCGTCATTGA TGGAAACACG ACTCAGCTGA ACTTTGTCAA TCTGAAGCCC 
GGATCGGTGG TAGCAGTGCG GTTTTCTCTG AAAAATAACA TGAAATCACC CATTGAGAAT 
CTACAGTCGT TGGTTCGAGA GTTCCACCAG GATAAGGGTT CTCGATATGT GGAACTGCAG 
CATATGCTGA GTAAGCTTAA CTTGGTGGAC TTCAACAGCT TGCTGTACTG TTGTGCTGAG 
GAGGAACGTG ACAATGGCGG AGGCCCGTAC AACATCTCTG GGTATGGTGA TTTGGTGTAC 
TGTGGACTTC AGGGCGTGGT TTCGATTTTA AGCGATATTG GTCCGAACAA TGATTTGGGT 
CATCCGTTGG CAAACAATCT GCGCATGGGA AATTGGTTGA TCGATTACTG TTCGCAGCGT 
CTGCAGAAAT ACCCAAACTT GGAACCGATT TCCAACTGGC TGGAGAAGAA TCTTGCTCCA 
CTTAAGGAAA TCCCTCGGTA CATGATTCCC AGCTACTTCG ATGTGATCAT CACTGGAGTT 
CACCGTTTGG CCATCAGTGC AGCGTGCAAC CTGATGTCGG AGTTCGTCCG GCAAGGCTCT 
AACTTTGGTC GCCGTTTGGC TCTGGGATCG GTTCAGTGCG TCAGGGTGGC TCCTTCAGCC 
AATCTTCCGC CAATGAGTCC GAATGTGAAC CCACCAAAGC CTCCACAGAG GTGTGCAACT 
CTGGCTGCTG GATTGCCACA CTTTGCGACC GGATATATGC GCTGCTGGGG ACGAGATACT 
TTCATTTCGT TGCGAGGACT TATGTTCCTT ACTGGACGTT TCGATGAGGC ACGCTATATG 
ATTCTTGGCT TTGGAGCATG TCTTCGGCAT GGATTGATTC CCAATCTCCT GGATGGAGGA 
ATGAATGCTC GTTTCAACTG CCGTGATGCC ATCTGGTGGT GGTTGTACTG CATTAGGCAA 
TACGTAGAAG AGGCTCCGAA TGGAAAGAAA ATACTGATGG ATAAGGTCTC CCGACTGTAT 
CCTACGGACG ACTCGGAACC GAAGATGCCT GGTGAATGCG ATCAATTCTT GTACGAGACG 
ATCCAGGAAG CGTTGACCGT ACACTTCCAG GGGTTGTCCT ACCGGGAACG CAATGCCGGC 
ACCCGTATCG ACGCCCACAT GAAGGACAAG GGATTCAACA ACCGGATCGG TATCCATCCG 
GAAACGGGAT TTGTCTTCGG TGGTAACAAT GCCAACTGCG GAACCTGGAT GGATAAGATG 
GGATCCAGTG ATAAGGCCGG TAATCGGGGT ATTCCCTCGT CGCCACGCGA TGGGTCTGCT 
GTGGAGCTGG TTGGTCTGCA GATGGCTGGT TTGAGATTTA TGCAGCGAAT GGCCGAAGAA 
AAGGCTATTC CTGGAACAGG TTCGGTGGAA CGCACTTCCA GCAATGGAAC CAAAACTGTT 
TGGACGTACA AGGAATGGGC CGACAAAATA GCGGCCAACT TTGAAAAAGA GTTCTACGTA 
GATGAAAGCA GCGACAGTAA GTACGTTAAT AAGAAGGGAA TCTACAAAGA CACATTGGGC 
TCAGAGATTC CATGGACCGA CTACCAACTG AGATGTAACT TCCCGATTGC ACTGGTAGCG 
GCCCCGGAGC TTTGCGACCA TAAACATGCC TGGAGGGCGT TGGAGAATGC CAAGAAATAT 
CTGCTGGGAC CCCTTGGTAT GAAGACCTTG GATCGCGAAG ATTGGGCCTA CCGTGGCAAT 
TATGACAATT CAATCGACAG TGACGACAAA ACTGTAGCGC ACGGAGCCAA CTATCACAAC 
GGTCCGGAAT GGGTTTGGCC AATAGGGTTC TATCTCCGTG CGAGACTTAT CTTCGCCAAG 
GCTAACGGAC TGCTCAAAGA GACGATCGCC GAAACGTGGT CCATCTTGCA AAAACATCTA 
CACGAACTGC AAACCAATCA TTGGCGCGGT CTGGCCGAGT TGACCAACGA AAATGGAGCG 
TACTGCAAAG ACTCGTGCCG GACACAGGCC TGGAGTATGG GTTGCGTGCT AGAAGTGTTG 
CATGACCTGG AAAAGTATGA AAAAGAGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001660447.1
CDS	131..4783
Translation	MLKKMGSFLSYEKLPTIFEVKKMADQTGSIKLAIHHNDQGKGSLYRLKKNSMVHVHPGPSLLGRRVAIFCNYPADGADFDRNRYTQLKWFSPTGTQLESDGSKYLSIKDVDYYCNVHLTRSGAYHFYILYEGEEEQQAGLYVQIEPSLTIGKGPSAAQLPLEAIRCQTVIAKLMGPLESWEAKLQVTKESGYNMVHFTPIQELGGSRSAYSLKNQNKVNPEFQNGDQPVEFEDVNKVIKKMREDWGIASICDIVLNHTANESVWIREYPECSYNCFTCPHLRPAFLLDALLARVGEDVGAGLLEHVGVPKVVETNDHLQAIHWQIHSNYLPQIKLYELYQCDVDKYVTRFNDEASKNGPSAAKEVPEGDLKLKHDQEYRRLGAQIDWDRAMKTYNVFRQDCFDEDSRKRKCAEAFRSKLQWLNEEVRKEIQAHLDYAIENCLAGARYERVQADGPQIKGISVKYPLFMKYFTQYGIQGKSLKDIEAMMYGGAGKLFMAHNGWVINSDPLKDFARPQEGTGNVYLRRELIAWGDSVKLRYGDKPEDSPYLWKHMKEYVDTTAKIFDGVRLDNCHSTPLHVAEYLLDSARKVNPELYVVAELFTNSDYTDNVFVNRLGITSLIREALSAWDSHEEGRLVYRYGGDPVGAFFPYPKRILAPSIAHALFMDLTHDNPSPVEKRSIFDLIPSAGLVSMACCATGSNRGYDELVPHHIHVVDEERQYQEWGKHVDKSTGIVAVKEAFNLLHGGLATRNFDQLFVDQMHPDIVAVTRHSTLNRETVVLVAHTAFNYPSPWAGPTGVRPLRVEGDLVEIIFEVQIYKKTGQTFDQPTEFKKDEHYINGVQEYVVDLRKNLKLDESDIFEKTPVIDGNTTQLNFVNLKPGSVVAVRFSLKNNMKSPIENLQSLVREFHQDKGSRYVELQHMLSKLNLVDFNSLLYCCAEEERDNGGGPYNISGYGDLVYCGLQGVVSILSDIGPNNDLGHPLANNLRMGNWLIDYCSQRLQKYPNLEPISNWLEKNLAPLKEIPRYMIPSYFDVIITGVHRLAISAACNLMSEFVRQGSNFGRRLALGSVQCVRVAPSANLPPMSPNVNPPKPPQRCATLAAGLPHFATGYMRCWGRDTFISLRGLMFLTGRFDEARYMILGFGACLRHGLIPNLLDGGMNARFNCRDAIWWWLYCIRQYVEEAPNGKKILMDKVSRLYPTDDSEPKMPGECDQFLYETIQEALTVHFQGLSYRERNAGTRIDAHMKDKGFNNRIGIHPETGFVFGGNNANCGTWMDKMGSSDKAGNRGIPSSPRDGSAVELVGLQMAGLRFMQRMAEEKAIPGTGSVERTSSNGTKTVWTYKEWADKIAANFEKEFYVDESSDSKYVNKKGIYKDTLGSEIPWTDYQLRCNFPIALVAAPELCDHKHAWRALENAKKYLLGPLGMKTLDREDWAYRGNYDNSIDSDDKTVAHGANYHNGPEWVWPIGFYLRARLIFAKANGLLKETIAETWSILQKHLHELQTNHWRGLAELTNENGAYCKDSCRTQAWSMGCVLEVLHDLEKYEKEL

Target ORF information:

RefSeq Version	XM_001660397.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti glycogen debranching enzyme (LOC5572509), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001660397.2

ORF Insert Sequence:

ATGTTGAAAA AGATGGGATC CTTTCTGTCT TATGAGAAAC TGCCGACAAT CTTCGAGGTG 
AAAAAGATGG CCGATCAGAC CGGTTCGATA AAGCTGGCGA TCCACCACAA TGACCAGGGC 
AAAGGCTCGC TCTACCGTCT GAAGAAGAAC TCCATGGTGC ACGTCCATCC GGGACCGTCT 
CTGCTGGGCC GCCGGGTGGC CATCTTCTGC AACTATCCGG CCGATGGTGC TGATTTCGAC 
CGCAACCGGT ACACCCAACT GAAGTGGTTC TCGCCAACGG GAACCCAGCT CGAGTCGGAT 
GGCTCCAAGT ATCTCTCGAT CAAGGATGTT GACTACTATT GCAATGTTCA CCTGACCCGC 
TCCGGGGCGT ACCACTTCTA CATTCTGTAC GAGGGTGAGG AAGAACAGCA GGCTGGACTG 
TATGTGCAAA TCGAGCCTTC TCTAACCATT GGGAAGGGTC CCTCAGCAGC CCAACTGCCC 
CTGGAGGCCA TCCGTTGTCA GACGGTCATC GCTAAGTTGA TGGGTCCTCT TGAAAGCTGG 
GAAGCCAAGT TGCAGGTCAC CAAGGAGAGC GGCTACAATA TGGTTCATTT CACACCGATC 
CAAGAGCTGG GCGGCTCGAG ATCTGCCTAT TCTCTCAAGA ATCAAAATAA AGTGAACCCG 
GAGTTCCAGA ATGGTGATCA ACCCGTTGAG TTCGAAGATG TGAATAAGGT TATCAAGAAA 
ATGCGCGAAG ATTGGGGTAT CGCGTCGATT TGCGATATCG TGCTGAATCA CACTGCGAAC 
GAGTCGGTTT GGATTCGGGA ATATCCGGAG TGCAGCTACA ATTGCTTCAC CTGCCCACAT 
TTGAGGCCGG CCTTCCTGTT GGATGCTTTA TTGGCTCGTG TGGGCGAAGA CGTGGGCGCT 
GGACTACTAG AGCATGTCGG TGTGCCAAAA GTGGTGGAGA CGAATGATCA TCTGCAGGCC 
ATCCATTGGC AGATCCACTC GAACTATCTG CCACAGATTA AGCTGTACGA GTTGTATCAG 
TGTGATGTAG ATAAGTATGT CACCAGGTTT AATGACGAGG CGTCGAAGAA TGGTCCGTCT 
GCGGCAAAAG AAGTACCGGA GGGTGACCTC AAGCTCAAGC ATGATCAGGA GTATAGACGG 
CTGGGAGCAC AAATCGATTG GGACCGGGCC ATGAAGACCT ACAATGTATT CCGCCAGGAC 
TGTTTCGACG AGGACAGTCG GAAGCGCAAA TGTGCAGAAG CATTCCGTTC GAAGTTGCAA 
TGGCTGAACG AGGAAGTTCG TAAGGAGATT CAGGCGCATT TGGATTATGC TATTGAGAAC 
TGCCTGGCTG GAGCTCGGTA TGAGCGAGTG CAAGCTGATG GACCTCAAAT CAAGGGAATT 
TCGGTGAAGT ATCCGTTGTT CATGAAGTAC TTCACTCAGT ACGGAATCCA GGGTAAATCG 
TTGAAGGATA TTGAAGCGAT GATGTACGGA GGAGCTGGTA AGTTGTTCAT GGCTCACAAT 
GGATGGGTGA TTAACAGTGA TCCGCTGAAG GACTTTGCTC GGCCTCAGGA GGGGACGGGT 
AATGTCTATT TGAGGAGGGA ATTGATTGCT TGGGGTGACA GCGTAAAGTT AAGGTACGGC 
GACAAACCGG AAGATAGCCC GTATTTGTGG AAGCACATGA AGGAGTATGT CGACACTACT 
GCGAAGATCT TCGACGGAGT TCGATTGGAC AACTGCCATT CGACGCCGCT GCATGTTGCG 
GAATATCTGC TGGATTCGGC GCGGAAGGTC AACCCGGAAT TGTACGTCGT GGCGGAACTG 
TTTACCAACT CTGATTACAC GGATAACGTT TTCGTCAATC GGTTGGGCAT TACTTCATTG 
ATTCGAGAGG CTTTGTCGGC ATGGGATTCC CACGAGGAAG GTCGACTCGT GTACCGATAT 
GGAGGTGATC CGGTGGGGGC GTTCTTCCCA TACCCGAAAC GTATTCTAGC GCCGAGCATA 
GCCCACGCAC TGTTCATGGA CCTGACGCAC GATAACCCAT CGCCAGTGGA GAAGCGATCG 
ATCTTCGATT TGATTCCGTC TGCTGGTTTG GTTTCTATGG CGTGCTGTGC GACGGGAAGC 
AATCGAGGGT ACGACGAGCT GGTTCCGCAT CACATTCACG TGGTCGACGA AGAACGTCAG 
TACCAGGAGT GGGGAAAACA CGTCGACAAA TCGACCGGCA TCGTGGCGGT TAAGGAAGCT 
TTCAATCTTT TGCACGGTGG TCTTGCGACG CGTAATTTCG ACCAGCTGTT CGTTGACCAA 
ATGCATCCGG ATATTGTGGC CGTCACCCGT CACTCTACGC TGAACCGCGA AACGGTTGTA 
TTGGTGGCGC ATACCGCGTT TAATTACCCC AGTCCTTGGG CTGGTCCGAC GGGAGTTCGC 
CCGCTGCGTG TCGAGGGCGA TTTGGTGGAG ATTATCTTCG AAGTACAGAT CTATAAGAAA 
ACTGGTCAAA CCTTCGATCA ACCCACGGAA TTTAAGAAAG ATGAACACTA CATCAACGGA 
GTCCAAGAAT ATGTGGTTGA TCTGCGCAAG AATTTAAAAC TGGATGAGTC GGATATCTTT 
GAGAAGACAC CCGTCATTGA TGGAAACACG ACTCAGCTGA ACTTTGTCAA TCTGAAGCCC 
GGATCGGTGG TAGCAGTGCG GTTTTCTCTG AAAAATAACA TGAAATCACC CATTGAGAAT 
CTACAGTCGT TGGTTCGAGA GTTCCACCAG GATAAGGGTT CTCGATATGT GGAACTGCAG 
CATATGCTGA GTAAGCTTAA CTTGGTGGAC TTCAACAGCT TGCTGTACTG TTGTGCTGAG 
GAGGAACGTG ACAATGGCGG AGGCCCGTAC AACATCTCTG GGTATGGTGA TTTGGTGTAC 
TGTGGACTTC AGGGCGTGGT TTCGATTTTA AGCGATATTG GTCCGAACAA TGATTTGGGT 
CATCCGTTGG CAAACAATCT GCGCATGGGA AATTGGTTGA TCGATTACTG TTCGCAGCGT 
CTGCAGAAAT ACCCAAACTT GGAACCGATT TCCAACTGGC TGGAGAAGAA TCTTGCTCCA 
CTTAAGGAAA TCCCTCGGTA CATGATTCCC AGCTACTTCG ATGTGATCAT CACTGGAGTT 
CACCGTTTGG CCATCAGTGC AGCGTGCAAC CTGATGTCGG AGTTCGTCCG GCAAGGCTCT 
AACTTTGGTC GCCGTTTGGC TCTGGGATCG GTTCAGTGCG TCAGGGTGGC TCCTTCAGCC 
AATCTTCCGC CAATGAGTCC GAATGTGAAC CCACCAAAGC CTCCACAGAG GTGTGCAACT 
CTGGCTGCTG GATTGCCACA CTTTGCGACC GGATATATGC GCTGCTGGGG ACGAGATACT 
TTCATTTCGT TGCGAGGACT TATGTTCCTT ACTGGACGTT TCGATGAGGC ACGCTATATG 
ATTCTTGGCT TTGGAGCATG TCTTCGGCAT GGATTGATTC CCAATCTCCT GGATGGAGGA 
ATGAATGCTC GTTTCAACTG CCGTGATGCC ATCTGGTGGT GGTTGTACTG CATTAGGCAA 
TACGTAGAAG AGGCTCCGAA TGGAAAGAAA ATACTGATGG ATAAGGTCTC CCGACTGTAT 
CCTACGGACG ACTCGGAACC GAAGATGCCT GGTGAATGCG ATCAATTCTT GTACGAGACG 
ATCCAGGAAG CGTTGACCGT ACACTTCCAG GGGTTGTCCT ACCGGGAACG CAATGCCGGC 
ACCCGTATCG ACGCCCACAT GAAGGACAAG GGATTCAACA ACCGGATCGG TATCCATCCG 
GAAACGGGAT TTGTCTTCGG TGGTAACAAT GCCAACTGCG GAACCTGGAT GGATAAGATG 
GGATCCAGTG ATAAGGCCGG TAATCGGGGT ATTCCCTCGT CGCCACGCGA TGGGTCTGCT 
GTGGAGCTGG TTGGTCTGCA GATGGCTGGT TTGAGATTTA TGCAGCGAAT GGCCGAAGAA 
AAGGCTATTC CTGGAACAGG TTCGGTGGAA CGCACTTCCA GCAATGGAAC CAAAACTGTT 
TGGACGTACA AGGAATGGGC CGACAAAATA GCGGCCAACT TTGAAAAAGA GTTCTACGTA 
GATGAAAGCA GCGACAGTAA GTACGTTAAT AAGAAGGGAA TCTACAAAGA CACATTGGGC 
TCAGAGATTC CATGGACCGA CTACCAACTG AGATGTAACT TCCCGATTGC ACTGGTAGCG 
GCCCCGGAGC TTTGCGACCA TAAACATGCC TGGAGGGCGT TGGAGAATGC CAAGAAATAT 
CTGCTGGGAC CCCTTGGTAT GAAGACCTTG GATCGCGAAG ATTGGGCCTA CCGTGGCAAT 
TATGACAATT CAATCGACAG TGACGACAAA ACTGTAGCGC ACGGAGCCAA CTATCACAAC 
GGTCCGGAAT GGGTTTGGCC AATAGGGTTC TATCTCCGTG CGAGACTTAT CTTCGCCAAG 
GCTAACGGAC TGCTCAAAGA GACGATCGCC GAAACGTGGT CCATCTTGCA AAAACATCTA 
CACGAACTGC AAACCAATCA TTGGCGCGGT CTGGCCGAGT TGACCAACGA AAATGGAGCG 
TACTGCAAAG ACTCGTGCCG GACACAGGCC TGGAGTATGG GTTGCGTGCT AGAAGTGTTG 
CATGACCTGG AAAAGTATGA AAAAGAGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5572509 ( XM_001660397.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001660447.1 Aedes aegypti AAEL009838-RA partial mRNA.

Price & Availability↑

CloneID	OAe07815
Clone ID Related Accession (Same CDS sequence)	XM_001660397.2 , XM_001660397.1
Accession Version	XM_001660397.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL009838-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811166). COMPLETENESS: incomplete on the 5' end.

ATGTTGAAAA AGATGGGATC CTTTCTGTCT TATGAGAAAC TGCCGACAAT CTTCGAGGTG 
AAAAAGATGG CCGATCAGAC CGGTTCGATA AAGCTGGCGA TCCACCACAA TGACCAGGGC 
AAAGGCTCGC TCTACCGTCT GAAGAAGAAC TCCATGGTGC ACGTCCATCC GGGACCGTCT 
CTGCTGGGCC GCCGGGTGGC CATCTTCTGC AACTATCCGG CCGATGGTGC TGATTTCGAC 
CGCAACCGGT ACACCCAACT GAAGTGGTTC TCGCCAACGG GAACCCAGCT CGAGTCGGAT 
GGCTCCAAGT ATCTCTCGAT CAAGGATGTT GACTACTATT GCAATGTTCA CCTGACCCGC 
TCCGGGGCGT ACCACTTCTA CATTCTGTAC GAGGGTGAGG AAGAACAGCA GGCTGGACTG 
TATGTGCAAA TCGAGCCTTC TCTAACCATT GGGAAGGGTC CCTCAGCAGC CCAACTGCCC 
CTGGAGGCCA TCCGTTGTCA GACGGTCATC GCTAAGTTGA TGGGTCCTCT TGAAAGCTGG 
GAAGCCAAGT TGCAGGTCAC CAAGGAGAGC GGCTACAATA TGGTTCATTT CACACCGATC 
CAAGAGCTGG GCGGCTCGAG ATCTGCCTAT TCTCTCAAGA ATCAAAATAA AGTGAACCCG 
GAGTTCCAGA ATGGTGATCA ACCCGTTGAG TTCGAAGATG TGAATAAGGT TATCAAGAAA 
ATGCGCGAAG ATTGGGGTAT CGCGTCGATT TGCGATATCG TGCTGAATCA CACTGCGAAC 
GAGTCGGTTT GGATTCGGGA ATATCCGGAG TGCAGCTACA ATTGCTTCAC CTGCCCACAT 
TTGAGGCCGG CCTTCCTGTT GGATGCTTTA TTGGCTCGTG TGGGCGAAGA CGTGGGCGCT 
GGACTACTAG AGCATGTCGG TGTGCCAAAA GTGGTGGAGA CGAATGATCA TCTGCAGGCC 
ATCCATTGGC AGATCCACTC GAACTATCTG CCACAGATTA AGCTGTACGA GTTGTATCAG 
TGTGATGTAG ATAAGTATGT CACCAGGTTT AATGACGAGG CGTCGAAGAA TGGTCCGTCT 
GCGGCAAAAG AAGTACCGGA GGGTGACCTC AAGCTCAAGC ATGATCAGGA GTATAGACGG 
CTGGGAGCAC AAATCGATTG GGACCGGGCC ATGAAGACCT ACAATGTATT CCGCCAGGAC 
TGTTTCGACG AGGACAGTCG GAAGCGCAAA TGTGCAGAAG CATTCCGTTC GAAGTTGCAA 
TGGCTGAACG AGGAAGTTCG TAAGGAGATT CAGGCGCATT TGGATTATGC TATTGAGAAC 
TGCCTGGCTG GAGCTCGGTA TGAGCGAGTG CAAGCTGATG GACCTCAAAT CAAGGGAATT 
TCGGTGAAGT ATCCGTTGTT CATGAAGTAC TTCACTCAGT ACGGAATCCA GGGTAAATCG 
TTGAAGGATA TTGAAGCGAT GATGTACGGA GGAGCTGGTA AGTTGTTCAT GGCTCACAAT 
GGATGGGTGA TTAACAGTGA TCCGCTGAAG GACTTTGCTC GGCCTCAGGA GGGGACGGGT 
AATGTCTATT TGAGGAGGGA ATTGATTGCT TGGGGTGACA GCGTAAAGTT AAGGTACGGC 
GACAAACCGG AAGATAGCCC GTATTTGTGG AAGCACATGA AGGAGTATGT CGACACTACT 
GCGAAGATCT TCGACGGAGT TCGATTGGAC AACTGCCATT CGACGCCGCT GCATGTTGCG 
GAATATCTGC TGGATTCGGC GCGGAAGGTC AACCCGGAAT TGTACGTCGT GGCGGAACTG 
TTTACCAACT CTGATTACAC GGATAACGTT TTCGTCAATC GGTTGGGCAT TACTTCATTG 
ATTCGAGAGG CTTTGTCGGC ATGGGATTCC CACGAGGAAG GTCGACTCGT GTACCGATAT 
GGAGGTGATC CGGTGGGGGC GTTCTTCCCA TACCCGAAAC GTATTCTAGC GCCGAGCATA 
GCCCACGCAC TGTTCATGGA CCTGACGCAC GATAACCCAT CGCCAGTGGA GAAGCGATCG 
ATCTTCGATT TGATTCCGTC TGCTGGTTTG GTTTCTATGG CGTGCTGTGC GACGGGAAGC 
AATCGAGGGT ACGACGAGCT GGTTCCGCAT CACATTCACG TGGTCGACGA AGAACGTCAG 
TACCAGGAGT GGGGAAAACA CGTCGACAAA TCGACCGGCA TCGTGGCGGT TAAGGAAGCT 
TTCAATCTTT TGCACGGTGG TCTTGCGACG CGTAATTTCG ACCAGCTGTT CGTTGACCAA 
ATGCATCCGG ATATTGTGGC CGTCACCCGT CACTCTACGC TGAACCGCGA AACGGTTGTA 
TTGGTGGCGC ATACCGCGTT TAATTACCCC AGTCCTTGGG CTGGTCCGAC GGGAGTTCGC 
CCGCTGCGTG TCGAGGGCGA TTTGGTGGAG ATTATCTTCG AAGTACAGAT CTATAAGAAA 
ACTGGTCAAA CCTTCGATCA ACCCACGGAA TTTAAGAAAG ATGAACACTA CATCAACGGA 
GTCCAAGAAT ATGTGGTTGA TCTGCGCAAG AATTTAAAAC TGGATGAGTC GGATATCTTT 
GAGAAGACAC CCGTCATTGA TGGAAACACG ACTCAGCTGA ACTTTGTCAA TCTGAAGCCC 
GGATCGGTGG TAGCAGTGCG GTTTTCTCTG AAAAATAACA TGAAATCACC CATTGAGAAT 
CTACAGTCGT TGGTTCGAGA GTTCCACCAG GATAAGGGTT CTCGATATGT GGAACTGCAG 
CATATGCTGA GTAAGCTTAA CTTGGTGGAC TTCAACAGCT TGCTGTACTG TTGTGCTGAG 
GAGGAACGTG ACAATGGCGG AGGCCCGTAC AACATCTCTG GGTATGGTGA TTTGGTGTAC 
TGTGGACTTC AGGGCGTGGT TTCGATTTTA AGCGATATTG GTCCGAACAA TGATTTGGGT 
CATCCGTTGG CAAACAATCT GCGCATGGGA AATTGGTTGA TCGATTACTG TTCGCAGCGT 
CTGCAGAAAT ACCCAAACTT GGAACCGATT TCCAACTGGC TGGAGAAGAA TCTTGCTCCA 
CTTAAGGAAA TCCCTCGGTA CATGATTCCC AGCTACTTCG ATGTGATCAT CACTGGAGTT 
CACCGTTTGG CCATCAGTGC AGCGTGCAAC CTGATGTCGG AGTTCGTCCG GCAAGGCTCT 
AACTTTGGTC GCCGTTTGGC TCTGGGATCG GTTCAGTGCG TCAGGGTGGC TCCTTCAGCC 
AATCTTCCGC CAATGAGTCC GAATGTGAAC CCACCAAAGC CTCCACAGAG GTGTGCAACT 
CTGGCTGCTG GATTGCCACA CTTTGCGACC GGATATATGC GCTGCTGGGG ACGAGATACT 
TTCATTTCGT TGCGAGGACT TATGTTCCTT ACTGGACGTT TCGATGAGGC ACGCTATATG 
ATTCTTGGCT TTGGAGCATG TCTTCGGCAT GGATTGATTC CCAATCTCCT GGATGGAGGA 
ATGAATGCTC GTTTCAACTG CCGTGATGCC ATCTGGTGGT GGTTGTACTG CATTAGGCAA 
TACGTAGAAG AGGCTCCGAA TGGAAAGAAA ATACTGATGG ATAAGGTCTC CCGACTGTAT 
CCTACGGACG ACTCGGAACC GAAGATGCCT GGTGAATGCG ATCAATTCTT GTACGAGACG 
ATCCAGGAAG CGTTGACCGT ACACTTCCAG GGGTTGTCCT ACCGGGAACG CAATGCCGGC 
ACCCGTATCG ACGCCCACAT GAAGGACAAG GGATTCAACA ACCGGATCGG TATCCATCCG 
GAAACGGGAT TTGTCTTCGG TGGTAACAAT GCCAACTGCG GAACCTGGAT GGATAAGATG 
GGATCCAGTG ATAAGGCCGG TAATCGGGGT ATTCCCTCGT CGCCACGCGA TGGGTCTGCT 
GTGGAGCTGG TTGGTCTGCA GATGGCTGGT TTGAGATTTA TGCAGCGAAT GGCCGAAGAA 
AAGGCTATTC CTGGAACAGG TTCGGTGGAA CGCACTTCCA GCAATGGAAC CAAAACTGTT 
TGGACGTACA AGGAATGGGC CGACAAAATA GCGGCCAACT TTGAAAAAGA GTTCTACGTA 
GATGAAAGCA GCGACAGTAA GTACGTTAAT AAGAAGGGAA TCTACAAAGA CACATTGGGC 
TCAGAGATTC CATGGACCGA CTACCAACTG AGATGTAACT TCCCGATTGC ACTGGTAGCG 
GCCCCGGAGC TTTGCGACCA TAAACATGCC TGGAGGGCGT TGGAGAATGC CAAGAAATAT 
CTGCTGGGAC CCCTTGGTAT GAAGACCTTG GATCGCGAAG ATTGGGCCTA CCGTGGCAAT 
TATGACAATT CAATCGACAG TGACGACAAA ACTGTAGCGC ACGGAGCCAA CTATCACAAC 
GGTCCGGAAT GGGTTTGGCC AATAGGGTTC TATCTCCGTG CGAGACTTAT CTTCGCCAAG 
GCTAACGGAC TGCTCAAAGA GACGATCGCC GAAACGTGGT CCATCTTGCA AAAACATCTA 
CACGAACTGC AAACCAATCA TTGGCGCGGT CTGGCCGAGT TGACCAACGA AAATGGAGCG 
TACTGCAAAG ACTCGTGCCG GACACAGGCC TGGAGTATGG GTTGCGTGCT AGAAGTGTTG 
CATGACCTGG AAAAGTATGA AAAAGAGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001660447.1
CDS	1..4653
Translation	MLKKMGSFLSYEKLPTIFEVKKMADQTGSIKLAIHHNDQGKGSLYRLKKNSMVHVHPGPSLLGRRVAIFCNYPADGADFDRNRYTQLKWFSPTGTQLESDGSKYLSIKDVDYYCNVHLTRSGAYHFYILYEGEEEQQAGLYVQIEPSLTIGKGPSAAQLPLEAIRCQTVIAKLMGPLESWEAKLQVTKESGYNMVHFTPIQELGGSRSAYSLKNQNKVNPEFQNGDQPVEFEDVNKVIKKMREDWGIASICDIVLNHTANESVWIREYPECSYNCFTCPHLRPAFLLDALLARVGEDVGAGLLEHVGVPKVVETNDHLQAIHWQIHSNYLPQIKLYELYQCDVDKYVTRFNDEASKNGPSAAKEVPEGDLKLKHDQEYRRLGAQIDWDRAMKTYNVFRQDCFDEDSRKRKCAEAFRSKLQWLNEEVRKEIQAHLDYAIENCLAGARYERVQADGPQIKGISVKYPLFMKYFTQYGIQGKSLKDIEAMMYGGAGKLFMAHNGWVINSDPLKDFARPQEGTGNVYLRRELIAWGDSVKLRYGDKPEDSPYLWKHMKEYVDTTAKIFDGVRLDNCHSTPLHVAEYLLDSARKVNPELYVVAELFTNSDYTDNVFVNRLGITSLIREALSAWDSHEEGRLVYRYGGDPVGAFFPYPKRILAPSIAHALFMDLTHDNPSPVEKRSIFDLIPSAGLVSMACCATGSNRGYDELVPHHIHVVDEERQYQEWGKHVDKSTGIVAVKEAFNLLHGGLATRNFDQLFVDQMHPDIVAVTRHSTLNRETVVLVAHTAFNYPSPWAGPTGVRPLRVEGDLVEIIFEVQIYKKTGQTFDQPTEFKKDEHYINGVQEYVVDLRKNLKLDESDIFEKTPVIDGNTTQLNFVNLKPGSVVAVRFSLKNNMKSPIENLQSLVREFHQDKGSRYVELQHMLSKLNLVDFNSLLYCCAEEERDNGGGPYNISGYGDLVYCGLQGVVSILSDIGPNNDLGHPLANNLRMGNWLIDYCSQRLQKYPNLEPISNWLEKNLAPLKEIPRYMIPSYFDVIITGVHRLAISAACNLMSEFVRQGSNFGRRLALGSVQCVRVAPSANLPPMSPNVNPPKPPQRCATLAAGLPHFATGYMRCWGRDTFISLRGLMFLTGRFDEARYMILGFGACLRHGLIPNLLDGGMNARFNCRDAIWWWLYCIRQYVEEAPNGKKILMDKVSRLYPTDDSEPKMPGECDQFLYETIQEALTVHFQGLSYRERNAGTRIDAHMKDKGFNNRIGIHPETGFVFGGNNANCGTWMDKMGSSDKAGNRGIPSSPRDGSAVELVGLQMAGLRFMQRMAEEKAIPGTGSVERTSSNGTKTVWTYKEWADKIAANFEKEFYVDESSDSKYVNKKGIYKDTLGSEIPWTDYQLRCNFPIALVAAPELCDHKHAWRALENAKKYLLGPLGMKTLDREDWAYRGNYDNSIDSDDKTVAHGANYHNGPEWVWPIGFYLRARLIFAKANGLLKETIAETWSILQKHLHELQTNHWRGLAELTNENGAYCKDSCRTQAWSMGCVLEVLHDLEKYEKEL

Target ORF information:

RefSeq Version	XM_001660397.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL009838-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001660397.1

ORF Insert Sequence:

ATGTTGAAAA AGATGGGATC CTTTCTGTCT TATGAGAAAC TGCCGACAAT CTTCGAGGTG 
AAAAAGATGG CCGATCAGAC CGGTTCGATA AAGCTGGCGA TCCACCACAA TGACCAGGGC 
AAAGGCTCGC TCTACCGTCT GAAGAAGAAC TCCATGGTGC ACGTCCATCC GGGACCGTCT 
CTGCTGGGCC GCCGGGTGGC CATCTTCTGC AACTATCCGG CCGATGGTGC TGATTTCGAC 
CGCAACCGGT ACACCCAACT GAAGTGGTTC TCGCCAACGG GAACCCAGCT CGAGTCGGAT 
GGCTCCAAGT ATCTCTCGAT CAAGGATGTT GACTACTATT GCAATGTTCA CCTGACCCGC 
TCCGGGGCGT ACCACTTCTA CATTCTGTAC GAGGGTGAGG AAGAACAGCA GGCTGGACTG 
TATGTGCAAA TCGAGCCTTC TCTAACCATT GGGAAGGGTC CCTCAGCAGC CCAACTGCCC 
CTGGAGGCCA TCCGTTGTCA GACGGTCATC GCTAAGTTGA TGGGTCCTCT TGAAAGCTGG 
GAAGCCAAGT TGCAGGTCAC CAAGGAGAGC GGCTACAATA TGGTTCATTT CACACCGATC 
CAAGAGCTGG GCGGCTCGAG ATCTGCCTAT TCTCTCAAGA ATCAAAATAA AGTGAACCCG 
GAGTTCCAGA ATGGTGATCA ACCCGTTGAG TTCGAAGATG TGAATAAGGT TATCAAGAAA 
ATGCGCGAAG ATTGGGGTAT CGCGTCGATT TGCGATATCG TGCTGAATCA CACTGCGAAC 
GAGTCGGTTT GGATTCGGGA ATATCCGGAG TGCAGCTACA ATTGCTTCAC CTGCCCACAT 
TTGAGGCCGG CCTTCCTGTT GGATGCTTTA TTGGCTCGTG TGGGCGAAGA CGTGGGCGCT 
GGACTACTAG AGCATGTCGG TGTGCCAAAA GTGGTGGAGA CGAATGATCA TCTGCAGGCC 
ATCCATTGGC AGATCCACTC GAACTATCTG CCACAGATTA AGCTGTACGA GTTGTATCAG 
TGTGATGTAG ATAAGTATGT CACCAGGTTT AATGACGAGG CGTCGAAGAA TGGTCCGTCT 
GCGGCAAAAG AAGTACCGGA GGGTGACCTC AAGCTCAAGC ATGATCAGGA GTATAGACGG 
CTGGGAGCAC AAATCGATTG GGACCGGGCC ATGAAGACCT ACAATGTATT CCGCCAGGAC 
TGTTTCGACG AGGACAGTCG GAAGCGCAAA TGTGCAGAAG CATTCCGTTC GAAGTTGCAA 
TGGCTGAACG AGGAAGTTCG TAAGGAGATT CAGGCGCATT TGGATTATGC TATTGAGAAC 
TGCCTGGCTG GAGCTCGGTA TGAGCGAGTG CAAGCTGATG GACCTCAAAT CAAGGGAATT 
TCGGTGAAGT ATCCGTTGTT CATGAAGTAC TTCACTCAGT ACGGAATCCA GGGTAAATCG 
TTGAAGGATA TTGAAGCGAT GATGTACGGA GGAGCTGGTA AGTTGTTCAT GGCTCACAAT 
GGATGGGTGA TTAACAGTGA TCCGCTGAAG GACTTTGCTC GGCCTCAGGA GGGGACGGGT 
AATGTCTATT TGAGGAGGGA ATTGATTGCT TGGGGTGACA GCGTAAAGTT AAGGTACGGC 
GACAAACCGG AAGATAGCCC GTATTTGTGG AAGCACATGA AGGAGTATGT CGACACTACT 
GCGAAGATCT TCGACGGAGT TCGATTGGAC AACTGCCATT CGACGCCGCT GCATGTTGCG 
GAATATCTGC TGGATTCGGC GCGGAAGGTC AACCCGGAAT TGTACGTCGT GGCGGAACTG 
TTTACCAACT CTGATTACAC GGATAACGTT TTCGTCAATC GGTTGGGCAT TACTTCATTG 
ATTCGAGAGG CTTTGTCGGC ATGGGATTCC CACGAGGAAG GTCGACTCGT GTACCGATAT 
GGAGGTGATC CGGTGGGGGC GTTCTTCCCA TACCCGAAAC GTATTCTAGC GCCGAGCATA 
GCCCACGCAC TGTTCATGGA CCTGACGCAC GATAACCCAT CGCCAGTGGA GAAGCGATCG 
ATCTTCGATT TGATTCCGTC TGCTGGTTTG GTTTCTATGG CGTGCTGTGC GACGGGAAGC 
AATCGAGGGT ACGACGAGCT GGTTCCGCAT CACATTCACG TGGTCGACGA AGAACGTCAG 
TACCAGGAGT GGGGAAAACA CGTCGACAAA TCGACCGGCA TCGTGGCGGT TAAGGAAGCT 
TTCAATCTTT TGCACGGTGG TCTTGCGACG CGTAATTTCG ACCAGCTGTT CGTTGACCAA 
ATGCATCCGG ATATTGTGGC CGTCACCCGT CACTCTACGC TGAACCGCGA AACGGTTGTA 
TTGGTGGCGC ATACCGCGTT TAATTACCCC AGTCCTTGGG CTGGTCCGAC GGGAGTTCGC 
CCGCTGCGTG TCGAGGGCGA TTTGGTGGAG ATTATCTTCG AAGTACAGAT CTATAAGAAA 
ACTGGTCAAA CCTTCGATCA ACCCACGGAA TTTAAGAAAG ATGAACACTA CATCAACGGA 
GTCCAAGAAT ATGTGGTTGA TCTGCGCAAG AATTTAAAAC TGGATGAGTC GGATATCTTT 
GAGAAGACAC CCGTCATTGA TGGAAACACG ACTCAGCTGA ACTTTGTCAA TCTGAAGCCC 
GGATCGGTGG TAGCAGTGCG GTTTTCTCTG AAAAATAACA TGAAATCACC CATTGAGAAT 
CTACAGTCGT TGGTTCGAGA GTTCCACCAG GATAAGGGTT CTCGATATGT GGAACTGCAG 
CATATGCTGA GTAAGCTTAA CTTGGTGGAC TTCAACAGCT TGCTGTACTG TTGTGCTGAG 
GAGGAACGTG ACAATGGCGG AGGCCCGTAC AACATCTCTG GGTATGGTGA TTTGGTGTAC 
TGTGGACTTC AGGGCGTGGT TTCGATTTTA AGCGATATTG GTCCGAACAA TGATTTGGGT 
CATCCGTTGG CAAACAATCT GCGCATGGGA AATTGGTTGA TCGATTACTG TTCGCAGCGT 
CTGCAGAAAT ACCCAAACTT GGAACCGATT TCCAACTGGC TGGAGAAGAA TCTTGCTCCA 
CTTAAGGAAA TCCCTCGGTA CATGATTCCC AGCTACTTCG ATGTGATCAT CACTGGAGTT 
CACCGTTTGG CCATCAGTGC AGCGTGCAAC CTGATGTCGG AGTTCGTCCG GCAAGGCTCT 
AACTTTGGTC GCCGTTTGGC TCTGGGATCG GTTCAGTGCG TCAGGGTGGC TCCTTCAGCC 
AATCTTCCGC CAATGAGTCC GAATGTGAAC CCACCAAAGC CTCCACAGAG GTGTGCAACT 
CTGGCTGCTG GATTGCCACA CTTTGCGACC GGATATATGC GCTGCTGGGG ACGAGATACT 
TTCATTTCGT TGCGAGGACT TATGTTCCTT ACTGGACGTT TCGATGAGGC ACGCTATATG 
ATTCTTGGCT TTGGAGCATG TCTTCGGCAT GGATTGATTC CCAATCTCCT GGATGGAGGA 
ATGAATGCTC GTTTCAACTG CCGTGATGCC ATCTGGTGGT GGTTGTACTG CATTAGGCAA 
TACGTAGAAG AGGCTCCGAA TGGAAAGAAA ATACTGATGG ATAAGGTCTC CCGACTGTAT 
CCTACGGACG ACTCGGAACC GAAGATGCCT GGTGAATGCG ATCAATTCTT GTACGAGACG 
ATCCAGGAAG CGTTGACCGT ACACTTCCAG GGGTTGTCCT ACCGGGAACG CAATGCCGGC 
ACCCGTATCG ACGCCCACAT GAAGGACAAG GGATTCAACA ACCGGATCGG TATCCATCCG 
GAAACGGGAT TTGTCTTCGG TGGTAACAAT GCCAACTGCG GAACCTGGAT GGATAAGATG 
GGATCCAGTG ATAAGGCCGG TAATCGGGGT ATTCCCTCGT CGCCACGCGA TGGGTCTGCT 
GTGGAGCTGG TTGGTCTGCA GATGGCTGGT TTGAGATTTA TGCAGCGAAT GGCCGAAGAA 
AAGGCTATTC CTGGAACAGG TTCGGTGGAA CGCACTTCCA GCAATGGAAC CAAAACTGTT 
TGGACGTACA AGGAATGGGC CGACAAAATA GCGGCCAACT TTGAAAAAGA GTTCTACGTA 
GATGAAAGCA GCGACAGTAA GTACGTTAAT AAGAAGGGAA TCTACAAAGA CACATTGGGC 
TCAGAGATTC CATGGACCGA CTACCAACTG AGATGTAACT TCCCGATTGC ACTGGTAGCG 
GCCCCGGAGC TTTGCGACCA TAAACATGCC TGGAGGGCGT TGGAGAATGC CAAGAAATAT 
CTGCTGGGAC CCCTTGGTAT GAAGACCTTG GATCGCGAAG ATTGGGCCTA CCGTGGCAAT 
TATGACAATT CAATCGACAG TGACGACAAA ACTGTAGCGC ACGGAGCCAA CTATCACAAC 
GGTCCGGAAT GGGTTTGGCC AATAGGGTTC TATCTCCGTG CGAGACTTAT CTTCGCCAAG 
GCTAACGGAC TGCTCAAAGA GACGATCGCC GAAACGTGGT CCATCTTGCA AAAACATCTA 
CACGAACTGC AAACCAATCA TTGGCGCGGT CTGGCCGAGT TGACCAACGA AAATGGAGCG 
TACTGCAAAG ACTCGTGCCG GACACAGGCC TGGAGTATGG GTTGCGTGCT AGAAGTGTTG 
CATGACCTGG AAAAGTATGA AAAAGAGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5572509 ( XM_021853942.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709634.1 Aedes aegypti glycogen debranching enzyme (LOC5572509), transcript variant X2, mRNA.

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CloneID	OAe128981
Clone ID Related Accession (Same CDS sequence)	XM_021853942.1
Accession Version	XM_021853942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATC AGACCGGTTC GATAAAGCTG GCGATCCACC ACAATGACCA GGGCAAAGGC 
TCGCTCTACC GTCTGAAGAA GAACTCCATG GTGCACGTCC ATCCGGGACC GTCTCTGCTG 
GGCCGCCGGG TGGCCATCTT CTGCAACTAT CCGGCCGATG GTGCTGATTT CGACCGCAAC 
CGGTACACCC AACTGAAGTG GTTCTCGCCA ACGGGAACCC AGCTCGAGTC GGATGGCTCC 
AAGTATCTCT CGATCAAGGA TGTTGACTAC TATTGCAATG TTCACCTGAC CCGCTCCGGG 
GCGTACCACT TCTACATTCT GTACGAGGGT GAGGAAGAAC AGCAGGCTGG ACTGTATGTG 
CAAATCGAGC CTTCTCTAAC CATTGGGAAG GGTCCCTCAG CAGCCCAACT GCCCCTGGAG 
GCCATCCGTT GTCAGACGGT CATCGCTAAG TTGATGGGTC CTCTTGAAAG CTGGGAAGCC 
AAGTTGCAGG TCACCAAGGA GAGCGGCTAC AATATGGTTC ATTTCACACC GATCCAAGAG 
CTGGGCGGCT CGAGATCTGC CTATTCTCTC AAGAATCAAA ATAAAGTGAA CCCGGAGTTC 
CAGAATGGTG ATCAACCCGT TGAGTTCGAA GATGTGAATA AGGTTATCAA GAAAATGCGC 
GAAGATTGGG GTATCGCGTC GATTTGCGAT ATCGTGCTGA ATCACACTGC GAACGAGTCG 
GTTTGGATTC GGGAATATCC GGAGTGCAGC TACAATTGCT TCACCTGCCC ACATTTGAGG 
CCGGCCTTCC TGTTGGATGC TTTATTGGCT CGTGTGGGCG AAGACGTGGG CGCTGGACTA 
CTAGAGCATG TCGGTGTGCC AAAAGTGGTG GAGACGAATG ATCATCTGCA GGCCATCCAT 
TGGCAGATCC ACTCGAACTA TCTGCCACAG ATTAAGCTGT ACGAGTTGTA TCAGTGTGAT 
GTAGATAAGT ATGTCACCAG GTTTAATGAC GAGGCGTCGA AGAATGGTCC GTCTGCGGCA 
AAAGAAGTAC CGGAGGGTGA CCTCAAGCTC AAGCATGATC AGGAGTATAG ACGGCTGGGA 
GCACAAATCG ATTGGGACCG GGCCATGAAG ACCTACAATG TATTCCGCCA GGACTGTTTC 
GACGAGGACA GTCGGAAGCG CAAATGTGCA GAAGCATTCC GTTCGAAGTT GCAATGGCTG 
AACGAGGAAG TTCGTAAGGA GATTCAGGCG CATTTGGATT ATGCTATTGA GAACTGCCTG 
GCTGGAGCTC GGTATGAGCG AGTGCAAGCT GATGGACCTC AAATCAAGGG AATTTCGGTG 
AAGTATCCGT TGTTCATGAA GTACTTCACT CAGTACGGAA TCCAGGGTAA ATCGTTGAAG 
GATATTGAAG CGATGATGTA CGGAGGAGCT GGTAAGTTGT TCATGGCTCA CAATGGATGG 
GTGATTAACA GTGATCCGCT GAAGGACTTT GCTCGGCCTC AGGAGGGGAC GGGTAATGTC 
TATTTGAGGA GGGAATTGAT TGCTTGGGGT GACAGCGTAA AGTTAAGGTA CGGCGACAAA 
CCGGAAGATA GCCCGTATTT GTGGAAGCAC ATGAAGGAGT ATGTCGACAC TACTGCGAAG 
ATCTTCGACG GAGTTCGATT GGACAACTGC CATTCGACGC CGCTGCATGT TGCGGAATAT 
CTGCTGGATT CGGCGCGGAA GGTCAACCCG GAATTGTACG TCGTGGCGGA ACTGTTTACC 
AACTCTGATT ACACGGATAA CGTTTTCGTC AATCGGTTGG GCATTACTTC ATTGATTCGA 
GAGGCTTTGT CGGCATGGGA TTCCCACGAG GAAGGTCGAC TCGTGTACCG ATATGGAGGT 
GATCCGGTGG GGGCGTTCTT CCCATACCCG AAACGTATTC TAGCGCCGAG CATAGCCCAC 
GCACTGTTCA TGGACCTGAC GCACGATAAC CCATCGCCAG TGGAGAAGCG ATCGATCTTC 
GATTTGATTC CGTCTGCTGG TTTGGTTTCT ATGGCGTGCT GTGCGACGGG AAGCAATCGA 
GGGTACGACG AGCTGGTTCC GCATCACATT CACGTGGTCG ACGAAGAACG TCAGTACCAG 
GAGTGGGGAA AACACGTCGA CAAATCGACC GGCATCGTGG CGGTTAAGGA AGCTTTCAAT 
CTTTTGCACG GTGGTCTTGC GACGCGTAAT TTCGACCAGC TGTTCGTTGA CCAAATGCAT 
CCGGATATTG TGGCCGTCAC CCGTCACTCT ACGCTGAACC GCGAAACGGT TGTATTGGTG 
GCGCATACCG CGTTTAATTA CCCCAGTCCT TGGGCTGGTC CGACGGGAGT TCGCCCGCTG 
CGTGTCGAGG GCGATTTGGT GGAGATTATC TTCGAAGTAC AGATCTATAA GAAAACTGGT 
CAAACCTTCG ATCAACCCAC GGAATTTAAG AAAGATGAAC ACTACATCAA CGGAGTCCAA 
GAATATGTGG TTGATCTGCG CAAGAATTTA AAACTGGATG AGTCGGATAT CTTTGAGAAG 
ACACCCGTCA TTGATGGAAA CACGACTCAG CTGAACTTTG TCAATCTGAA GCCCGGATCG 
GTGGTAGCAG TGCGGTTTTC TCTGAAAAAT AACATGAAAT CACCCATTGA GAATCTACAG 
TCGTTGGTTC GAGAGTTCCA CCAGGATAAG GGTTCTCGAT ATGTGGAACT GCAGCATATG 
CTGAGTAAGC TTAACTTGGT GGACTTCAAC AGCTTGCTGT ACTGTTGTGC TGAGGAGGAA 
CGTGACAATG GCGGAGGCCC GTACAACATC TCTGGGTATG GTGATTTGGT GTACTGTGGA 
CTTCAGGGCG TGGTTTCGAT TTTAAGCGAT ATTGGTCCGA ACAATGATTT GGGTCATCCG 
TTGGCAAACA ATCTGCGCAT GGGAAATTGG TTGATCGATT ACTGTTCGCA GCGTCTGCAG 
AAATACCCAA ACTTGGAACC GATTTCCAAC TGGCTGGAGA AGAATCTTGC TCCACTTAAG 
GAAATCCCTC GGTACATGAT TCCCAGCTAC TTCGATGTGA TCATCACTGG AGTTCACCGT 
TTGGCCATCA GTGCAGCGTG CAACCTGATG TCGGAGTTCG TCCGGCAAGG CTCTAACTTT 
GGTCGCCGTT TGGCTCTGGG ATCGGTTCAG TGCGTCAGGG TGGCTCCTTC AGCCAATCTT 
CCGCCAATGA GTCCGAATGT GAACCCACCA AAGCCTCCAC AGAGGTGTGC AACTCTGGCT 
GCTGGATTGC CACACTTTGC GACCGGATAT ATGCGCTGCT GGGGACGAGA TACTTTCATT 
TCGTTGCGAG GACTTATGTT CCTTACTGGA CGTTTCGATG AGGCACGCTA TATGATTCTT 
GGCTTTGGAG CATGTCTTCG GCATGGATTG ATTCCCAATC TCCTGGATGG AGGAATGAAT 
GCTCGTTTCA ACTGCCGTGA TGCCATCTGG TGGTGGTTGT ACTGCATTAG GCAATACGTA 
GAAGAGGCTC CGAATGGAAA GAAAATACTG ATGGATAAGG TCTCCCGACT GTATCCTACG 
GACGACTCGG AACCGAAGAT GCCTGGTGAA TGCGATCAAT TCTTGTACGA GACGATCCAG 
GAAGCGTTGA CCGTACACTT CCAGGGGTTG TCCTACCGGG AACGCAATGC CGGCACCCGT 
ATCGACGCCC ACATGAAGGA CAAGGGATTC AACAACCGGA TCGGTATCCA TCCGGAAACG 
GGATTTGTCT TCGGTGGTAA CAATGCCAAC TGCGGAACCT GGATGGATAA GATGGGATCC 
AGTGATAAGG CCGGTAATCG GGGTATTCCC TCGTCGCCAC GCGATGGGTC TGCTGTGGAG 
CTGGTTGGTC TGCAGATGGC TGGTTTGAGA TTTATGCAGC GAATGGCCGA AGAAAAGGCT 
ATTCCTGGAA CAGGTTCGGT GGAACGCACT TCCAGCAATG GAACCAAAAC TGTTTGGACG 
TACAAGGAAT GGGCCGACAA AATAGCGGCC AACTTTGAAA AAGAGTTCTA CGTAGATGAA 
AGCAGCGACA GTAAGTACGT TAATAAGAAG GGAATCTACA AAGACACATT GGGCTCAGAG 
ATTCCATGGA CCGACTACCA ACTGAGATGT AACTTCCCGA TTGCACTGGT AGCGGCCCCG 
GAGCTTTGCG ACCATAAACA TGCCTGGAGG GCGTTGGAGA ATGCCAAGAA ATATCTGCTG 
GGACCCCTTG GTATGAAGAC CTTGGATCGC GAAGATTGGG CCTACCGTGG CAATTATGAC 
AATTCAATCG ACAGTGACGA CAAAACTGTA GCGCACGGAG CCAACTATCA CAACGGTCCG 
GAATGGGTTT GGCCAATAGG GTTCTATCTC CGTGCGAGAC TTATCTTCGC CAAGGCTAAC 
GGACTGCTCA AAGAGACGAT CGCCGAAACG TGGTCCATCT TGCAAAAACA TCTACACGAA 
CTGCAAACCA ATCATTGGCG CGGTCTGGCC GAGTTGACCA ACGAAAATGG AGCGTACTGC 
AAAGACTCGT GCCGGACACA GGCCTGGAGT ATGGGTTGCG TGCTAGAAGT GTTGCATGAC 
CTGGAAAAGT ATGAAAAAGA GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709634.1
CDS	177..4763
Translation	MADQTGSIKLAIHHNDQGKGSLYRLKKNSMVHVHPGPSLLGRRVAIFCNYPADGADFDRNRYTQLKWFSPTGTQLESDGSKYLSIKDVDYYCNVHLTRSGAYHFYILYEGEEEQQAGLYVQIEPSLTIGKGPSAAQLPLEAIRCQTVIAKLMGPLESWEAKLQVTKESGYNMVHFTPIQELGGSRSAYSLKNQNKVNPEFQNGDQPVEFEDVNKVIKKMREDWGIASICDIVLNHTANESVWIREYPECSYNCFTCPHLRPAFLLDALLARVGEDVGAGLLEHVGVPKVVETNDHLQAIHWQIHSNYLPQIKLYELYQCDVDKYVTRFNDEASKNGPSAAKEVPEGDLKLKHDQEYRRLGAQIDWDRAMKTYNVFRQDCFDEDSRKRKCAEAFRSKLQWLNEEVRKEIQAHLDYAIENCLAGARYERVQADGPQIKGISVKYPLFMKYFTQYGIQGKSLKDIEAMMYGGAGKLFMAHNGWVINSDPLKDFARPQEGTGNVYLRRELIAWGDSVKLRYGDKPEDSPYLWKHMKEYVDTTAKIFDGVRLDNCHSTPLHVAEYLLDSARKVNPELYVVAELFTNSDYTDNVFVNRLGITSLIREALSAWDSHEEGRLVYRYGGDPVGAFFPYPKRILAPSIAHALFMDLTHDNPSPVEKRSIFDLIPSAGLVSMACCATGSNRGYDELVPHHIHVVDEERQYQEWGKHVDKSTGIVAVKEAFNLLHGGLATRNFDQLFVDQMHPDIVAVTRHSTLNRETVVLVAHTAFNYPSPWAGPTGVRPLRVEGDLVEIIFEVQIYKKTGQTFDQPTEFKKDEHYINGVQEYVVDLRKNLKLDESDIFEKTPVIDGNTTQLNFVNLKPGSVVAVRFSLKNNMKSPIENLQSLVREFHQDKGSRYVELQHMLSKLNLVDFNSLLYCCAEEERDNGGGPYNISGYGDLVYCGLQGVVSILSDIGPNNDLGHPLANNLRMGNWLIDYCSQRLQKYPNLEPISNWLEKNLAPLKEIPRYMIPSYFDVIITGVHRLAISAACNLMSEFVRQGSNFGRRLALGSVQCVRVAPSANLPPMSPNVNPPKPPQRCATLAAGLPHFATGYMRCWGRDTFISLRGLMFLTGRFDEARYMILGFGACLRHGLIPNLLDGGMNARFNCRDAIWWWLYCIRQYVEEAPNGKKILMDKVSRLYPTDDSEPKMPGECDQFLYETIQEALTVHFQGLSYRERNAGTRIDAHMKDKGFNNRIGIHPETGFVFGGNNANCGTWMDKMGSSDKAGNRGIPSSPRDGSAVELVGLQMAGLRFMQRMAEEKAIPGTGSVERTSSNGTKTVWTYKEWADKIAANFEKEFYVDESSDSKYVNKKGIYKDTLGSEIPWTDYQLRCNFPIALVAAPELCDHKHAWRALENAKKYLLGPLGMKTLDREDWAYRGNYDNSIDSDDKTVAHGANYHNGPEWVWPIGFYLRARLIFAKANGLLKETIAETWSILQKHLHELQTNHWRGLAELTNENGAYCKDSCRTQAWSMGCVLEVLHDLEKYEKEL

Target ORF information:

RefSeq Version	XM_021853942.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti glycogen debranching enzyme (LOC5572509), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021853942.1

ORF Insert Sequence:

ATGGCCGATC AGACCGGTTC GATAAAGCTG GCGATCCACC ACAATGACCA GGGCAAAGGC 
TCGCTCTACC GTCTGAAGAA GAACTCCATG GTGCACGTCC ATCCGGGACC GTCTCTGCTG 
GGCCGCCGGG TGGCCATCTT CTGCAACTAT CCGGCCGATG GTGCTGATTT CGACCGCAAC 
CGGTACACCC AACTGAAGTG GTTCTCGCCA ACGGGAACCC AGCTCGAGTC GGATGGCTCC 
AAGTATCTCT CGATCAAGGA TGTTGACTAC TATTGCAATG TTCACCTGAC CCGCTCCGGG 
GCGTACCACT TCTACATTCT GTACGAGGGT GAGGAAGAAC AGCAGGCTGG ACTGTATGTG 
CAAATCGAGC CTTCTCTAAC CATTGGGAAG GGTCCCTCAG CAGCCCAACT GCCCCTGGAG 
GCCATCCGTT GTCAGACGGT CATCGCTAAG TTGATGGGTC CTCTTGAAAG CTGGGAAGCC 
AAGTTGCAGG TCACCAAGGA GAGCGGCTAC AATATGGTTC ATTTCACACC GATCCAAGAG 
CTGGGCGGCT CGAGATCTGC CTATTCTCTC AAGAATCAAA ATAAAGTGAA CCCGGAGTTC 
CAGAATGGTG ATCAACCCGT TGAGTTCGAA GATGTGAATA AGGTTATCAA GAAAATGCGC 
GAAGATTGGG GTATCGCGTC GATTTGCGAT ATCGTGCTGA ATCACACTGC GAACGAGTCG 
GTTTGGATTC GGGAATATCC GGAGTGCAGC TACAATTGCT TCACCTGCCC ACATTTGAGG 
CCGGCCTTCC TGTTGGATGC TTTATTGGCT CGTGTGGGCG AAGACGTGGG CGCTGGACTA 
CTAGAGCATG TCGGTGTGCC AAAAGTGGTG GAGACGAATG ATCATCTGCA GGCCATCCAT 
TGGCAGATCC ACTCGAACTA TCTGCCACAG ATTAAGCTGT ACGAGTTGTA TCAGTGTGAT 
GTAGATAAGT ATGTCACCAG GTTTAATGAC GAGGCGTCGA AGAATGGTCC GTCTGCGGCA 
AAAGAAGTAC CGGAGGGTGA CCTCAAGCTC AAGCATGATC AGGAGTATAG ACGGCTGGGA 
GCACAAATCG ATTGGGACCG GGCCATGAAG ACCTACAATG TATTCCGCCA GGACTGTTTC 
GACGAGGACA GTCGGAAGCG CAAATGTGCA GAAGCATTCC GTTCGAAGTT GCAATGGCTG 
AACGAGGAAG TTCGTAAGGA GATTCAGGCG CATTTGGATT ATGCTATTGA GAACTGCCTG 
GCTGGAGCTC GGTATGAGCG AGTGCAAGCT GATGGACCTC AAATCAAGGG AATTTCGGTG 
AAGTATCCGT TGTTCATGAA GTACTTCACT CAGTACGGAA TCCAGGGTAA ATCGTTGAAG 
GATATTGAAG CGATGATGTA CGGAGGAGCT GGTAAGTTGT TCATGGCTCA CAATGGATGG 
GTGATTAACA GTGATCCGCT GAAGGACTTT GCTCGGCCTC AGGAGGGGAC GGGTAATGTC 
TATTTGAGGA GGGAATTGAT TGCTTGGGGT GACAGCGTAA AGTTAAGGTA CGGCGACAAA 
CCGGAAGATA GCCCGTATTT GTGGAAGCAC ATGAAGGAGT ATGTCGACAC TACTGCGAAG 
ATCTTCGACG GAGTTCGATT GGACAACTGC CATTCGACGC CGCTGCATGT TGCGGAATAT 
CTGCTGGATT CGGCGCGGAA GGTCAACCCG GAATTGTACG TCGTGGCGGA ACTGTTTACC 
AACTCTGATT ACACGGATAA CGTTTTCGTC AATCGGTTGG GCATTACTTC ATTGATTCGA 
GAGGCTTTGT CGGCATGGGA TTCCCACGAG GAAGGTCGAC TCGTGTACCG ATATGGAGGT 
GATCCGGTGG GGGCGTTCTT CCCATACCCG AAACGTATTC TAGCGCCGAG CATAGCCCAC 
GCACTGTTCA TGGACCTGAC GCACGATAAC CCATCGCCAG TGGAGAAGCG ATCGATCTTC 
GATTTGATTC CGTCTGCTGG TTTGGTTTCT ATGGCGTGCT GTGCGACGGG AAGCAATCGA 
GGGTACGACG AGCTGGTTCC GCATCACATT CACGTGGTCG ACGAAGAACG TCAGTACCAG 
GAGTGGGGAA AACACGTCGA CAAATCGACC GGCATCGTGG CGGTTAAGGA AGCTTTCAAT 
CTTTTGCACG GTGGTCTTGC GACGCGTAAT TTCGACCAGC TGTTCGTTGA CCAAATGCAT 
CCGGATATTG TGGCCGTCAC CCGTCACTCT ACGCTGAACC GCGAAACGGT TGTATTGGTG 
GCGCATACCG CGTTTAATTA CCCCAGTCCT TGGGCTGGTC CGACGGGAGT TCGCCCGCTG 
CGTGTCGAGG GCGATTTGGT GGAGATTATC TTCGAAGTAC AGATCTATAA GAAAACTGGT 
CAAACCTTCG ATCAACCCAC GGAATTTAAG AAAGATGAAC ACTACATCAA CGGAGTCCAA 
GAATATGTGG TTGATCTGCG CAAGAATTTA AAACTGGATG AGTCGGATAT CTTTGAGAAG 
ACACCCGTCA TTGATGGAAA CACGACTCAG CTGAACTTTG TCAATCTGAA GCCCGGATCG 
GTGGTAGCAG TGCGGTTTTC TCTGAAAAAT AACATGAAAT CACCCATTGA GAATCTACAG 
TCGTTGGTTC GAGAGTTCCA CCAGGATAAG GGTTCTCGAT ATGTGGAACT GCAGCATATG 
CTGAGTAAGC TTAACTTGGT GGACTTCAAC AGCTTGCTGT ACTGTTGTGC TGAGGAGGAA 
CGTGACAATG GCGGAGGCCC GTACAACATC TCTGGGTATG GTGATTTGGT GTACTGTGGA 
CTTCAGGGCG TGGTTTCGAT TTTAAGCGAT ATTGGTCCGA ACAATGATTT GGGTCATCCG 
TTGGCAAACA ATCTGCGCAT GGGAAATTGG TTGATCGATT ACTGTTCGCA GCGTCTGCAG 
AAATACCCAA ACTTGGAACC GATTTCCAAC TGGCTGGAGA AGAATCTTGC TCCACTTAAG 
GAAATCCCTC GGTACATGAT TCCCAGCTAC TTCGATGTGA TCATCACTGG AGTTCACCGT 
TTGGCCATCA GTGCAGCGTG CAACCTGATG TCGGAGTTCG TCCGGCAAGG CTCTAACTTT 
GGTCGCCGTT TGGCTCTGGG ATCGGTTCAG TGCGTCAGGG TGGCTCCTTC AGCCAATCTT 
CCGCCAATGA GTCCGAATGT GAACCCACCA AAGCCTCCAC AGAGGTGTGC AACTCTGGCT 
GCTGGATTGC CACACTTTGC GACCGGATAT ATGCGCTGCT GGGGACGAGA TACTTTCATT 
TCGTTGCGAG GACTTATGTT CCTTACTGGA CGTTTCGATG AGGCACGCTA TATGATTCTT 
GGCTTTGGAG CATGTCTTCG GCATGGATTG ATTCCCAATC TCCTGGATGG AGGAATGAAT 
GCTCGTTTCA ACTGCCGTGA TGCCATCTGG TGGTGGTTGT ACTGCATTAG GCAATACGTA 
GAAGAGGCTC CGAATGGAAA GAAAATACTG ATGGATAAGG TCTCCCGACT GTATCCTACG 
GACGACTCGG AACCGAAGAT GCCTGGTGAA TGCGATCAAT TCTTGTACGA GACGATCCAG 
GAAGCGTTGA CCGTACACTT CCAGGGGTTG TCCTACCGGG AACGCAATGC CGGCACCCGT 
ATCGACGCCC ACATGAAGGA CAAGGGATTC AACAACCGGA TCGGTATCCA TCCGGAAACG 
GGATTTGTCT TCGGTGGTAA CAATGCCAAC TGCGGAACCT GGATGGATAA GATGGGATCC 
AGTGATAAGG CCGGTAATCG GGGTATTCCC TCGTCGCCAC GCGATGGGTC TGCTGTGGAG 
CTGGTTGGTC TGCAGATGGC TGGTTTGAGA TTTATGCAGC GAATGGCCGA AGAAAAGGCT 
ATTCCTGGAA CAGGTTCGGT GGAACGCACT TCCAGCAATG GAACCAAAAC TGTTTGGACG 
TACAAGGAAT GGGCCGACAA AATAGCGGCC AACTTTGAAA AAGAGTTCTA CGTAGATGAA 
AGCAGCGACA GTAAGTACGT TAATAAGAAG GGAATCTACA AAGACACATT GGGCTCAGAG 
ATTCCATGGA CCGACTACCA ACTGAGATGT AACTTCCCGA TTGCACTGGT AGCGGCCCCG 
GAGCTTTGCG ACCATAAACA TGCCTGGAGG GCGTTGGAGA ATGCCAAGAA ATATCTGCTG 
GGACCCCTTG GTATGAAGAC CTTGGATCGC GAAGATTGGG CCTACCGTGG CAATTATGAC 
AATTCAATCG ACAGTGACGA CAAAACTGTA GCGCACGGAG CCAACTATCA CAACGGTCCG 
GAATGGGTTT GGCCAATAGG GTTCTATCTC CGTGCGAGAC TTATCTTCGC CAAGGCTAAC 
GGACTGCTCA AAGAGACGAT CGCCGAAACG TGGTCCATCT TGCAAAAACA TCTACACGAA 
CTGCAAACCA ATCATTGGCG CGGTCTGGCC GAGTTGACCA ACGAAAATGG AGCGTACTGC 
AAAGACTCGT GCCGGACACA GGCCTGGAGT ATGGGTTGCG TGCTAGAAGT GTTGCATGAC 
CTGGAAAAGT ATGAAAAAGA GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5572509 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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