LOC5572305 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5572305
Entrez Gene ID	5572305
Full Name	ubiquitin carboxyl-terminal hydrolase 20
Synonyms	AAEL009702
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001653949.1	XP_001653999.1	AAEL009702-PA
XM_001653949.2	XP_001653999.2	ubiquitin carboxyl-terminal hydrolase 20 isoform X2
XM_021854463.1	XP_021710155.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
XM_021854464.1	XP_021710156.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5572305 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5572305 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe07639	XM_001653949.1 Latest version!	Aedes aegypti AAEL009702-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe129332	XM_021854463.1 Latest version!	Aedes aegypti ubiquitin carboxyl-terminal hydrolase 20 (LOC5572305), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe129333	XM_001653949.2 Latest version!	Aedes aegypti ubiquitin carboxyl-terminal hydrolase 20 (LOC5572305), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe129334	XM_021854464.1 Latest version!	Aedes aegypti ubiquitin carboxyl-terminal hydrolase 20 (LOC5572305), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5572305 ( XM_001653949.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001653999.1 Aedes aegypti AAEL009702-RA partial mRNA.

Price & Availability↑

CloneID	OAe07639
Clone ID Related Accession (Same CDS sequence)	XM_001653949.1
Accession Version	XM_001653949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL009702-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811153). COMPLETENESS: incomplete on the 5' end.

ATGGCTCGAA GTGGGGGCAG CAAGTGTTCT CATCTGGACA ATGTTGTTCA TCTCTCGTTG 
GTCGAGCTGT GCAAACTCAA GAAGGGTGAA CCATGTACGG AGTGCGACGC CAACGGTCCA 
AACCTGTGGA TATGTCTGCA GAAAAATTGT CTCCACATTG GCTGCTCGGA GCAGTACAAC 
GACCACAGTA CGACCCACTT CCAAACGAAC CGCACCCATT GCATCCACAT GAACCTGTCC 
TCTCAAAGGA TATGGTGCTA TTTGTGCGAG TGCGAAGTGT TCCTGGTGCA GCAACAACAA 
CAGCAACAGC AGCAGCTGAG ACGAACCTCG GTGGTGAGCA ACGATTCGAG TGATGCCAGT 
CGATACTCGG CCAACAACAA TAACGAGAAG CTGCTGATGT ATGGGGATCG GTTTGCCAGT 
GGATTTGGAG GTGGCGAATC CTGTGATAGC AGCTGCGAAG AAGACGAGTT TGAGAAGCAT 
GGTAAATGGA ATGGGCTGGT CGGGTTACAG AATATAGCAA ATACTTGCTA TATGAACTCC 
GCAATCCAAG CACTGAGCAA CAGTCCTCCC TTGACGGGAT ACTTCCTGGA TTGTGGGTCG 
ATCATAGAAA CCGATTTGCT CGGAAATCAA ACCGTTTCAA AGCCAGGTTT GGCCAAGCAT 
TACCACAAGT TGATCAAGGA AATGTGGTGT AAGAACAGGA GGTATGTCGT CCCCAGTGGA 
ATCCTGTATG GAATTCGAAT AGTACATCCC ATGTTTCGAG GCTATCAGCA ACATGACACC 
CAAGAATTTC TCCGCTGCTT CATGGATCAG CTACATGAAG AGTTGAAGGA AGTTATTCAA 
CCGCCGCCAG AGCTGCTATC ACTGCATGGT AGAAACGATG AAGACAATGA AGATCATTCG 
CCTTGTCCTT CGCCATCGCC TTCGCAATCC GAGGCAGAGT ATGAAACATG TGACAGCGGT 
GTTTCGGAGC AATCGAGCCT CTCGGATGAT ATATCAGTTA ATAATAAACG AACTAGAATG 
ATTTCGAGAT CTCCTAGCCC CACCGCAAAC CAAAGAAGTG CAACCTCGAA CAGTCGGCAA 
CAATCGCCGA TAGGAAGCAA TCGGAGCAAC TCCAGCAGCA GTGGAAGCAT GTCTTCTATG 
CAGCAGCAGC AGCAAGCCGG CTCCAGTGAT CAGCAACAAC AGAAACCTGC AAAGGACAAA 
CTTCCGGAAC GGTCCATCAT CAGCGACATA TTTGATGGGA AGCTTCTCTC ATCTGTACAA 
TGCTTAACCT GCGATCGTAT TTCAACTCGT GAAGAAACAT TTCAGGATCT ATCGTTACCA 
ATCCCCGGCA AAGATCATCT GGCAGTGTTG CATCAGAACC ACCCGGGAAT GCCTTCTCCT 
TCAGCTCATC CTTCGTCTCC GGTGGCTGGA AGTAGCAGTG GAATCACATG CTCTGATGCG 
GTCTATCCGG TAACCGGCGA TAGCTGGATG TGGTGGTTTT GGAACTGGCC TTTTTGGAGT 
TGGGTTCGAT CATGGTTCTG GGGACCAGCC GTTACACTGC ATGACTGCAT GGCAGCCTTC 
TTCAGTGCGG ACGAGCTGAA AGGAGACAAC ATGTACAGCT GCGAAAAGTG TAACAAACTC 
CGGAATGGGG TGAAGTTTTC CCGTGTTCTT GCCTTGCCTG AAATGTTGTG CGTCCATTTG 
AAACGTTTCC GGCACGATCT CTCCTACAGC TCGAAAATAT CCAGCCCCGT TAATTTCCCC 
ATGAACGGAC TAGACATGAG GCCATATCTG CACAAGGACT GCAAATCGGA AGTGACCTCG 
TACGATCTCG GTGCGGTCAT TTGTCACCAC GGCACGGTGG GAGGCGGACA TTATACCAGC 
TTTGCGAAAC ACGAACCCAC CGGGAAATGG TTCGAGTTCG ATGACCAGCT GGTGACGCAA 
GTCAGCCCGG AGGATGTCCA GAACTGTGAA GCTTATGTGC TGTTCTATCG CAAGAACAAC 
AGCAAAATGA GTACGATTCG GGCACAGGCC AGTGATCTGA TCAGCGTCCA GGAGCACGCT 
TCTGACATAA GGTTCTACAT TTCCCGGAAT TGGTTGCACA GGTTCAACAC GTTTGCTGAA 
CCCGGACCGA TAGACAACTG GACGTTGCTC TGTCCTCATG GCGCGTTTCC ACCACACAAA 
GCTCCGTACT TTTCGAAGCT GGTAGTGCCA ATCCCGCAGA TCTTGTGGGA CTTTCTGTAC 
AAAAAGTTCG GTGGAGGCCC GGTGTGCAAC CACATCTTCC CGTGTCATAT TTGCAAGAAG 
GCAGCCGAAT CGTTGTCCAG GAGGCAACGA GCCGAGCTGG AAGAATTCAC CGCCTGTAAT 
GACGAGTATC AGTACAATGA TACTCCAACC ACGATCTACG CGATCTCGAT GGCATGGTTC 
CGACAGTGGC AACTGTTTGC TCGCGGTGTC ACCACGGAAG AACCAGGACC TATCGATAAC 
AAAACCATTG CAGTTCCGGG GGATACAAAT GTTCCGCTTC GAAGCGTGCG CCAAGGATCG 
GACTACGCCC AGATCAACTC GGCCTTGTGG CACTTTTTCC ACGGCATCTA CGGTGGAGGG 
CCGGAAATCG TACTCCGCGG TAATCCGGTT CCGCCGCCAG AACCGAAAAT CCCGAAAGTG 
GCGAAGGAAC GAGAAATTGA ACCCATGGAC GTGAGTGAAC CAACTCCACC AGTGAAAACC 
ACTGCTCCGG CAAGCGTGAC CCAGCAGCAT CAGCATCAGA AGGAGCATTC CGCTGAAGCG 
ACCACAGTGG ATCCATCTCC AAGTACCCAA TCTACAACGG CGAAACCCCA GAAAAGTGTC 
TCCTTTGAGG ACAACGAAGG TTACCACGAA AACGACGACG ATCATAACGA ATCGTCTCGA 
TCAGTGCAGT TGAAGCGCAA GCAGCTGAAG CAGCAGTCGA AGGAATCGAA GAAATCCGAA 
TCCGACAGCG CCGATGGCCT GGTGACCTAT CTTCGGCGGA AGCAACTCAA GCACCAACAG 
CACGTGGCCG CTAGCCAAGG GGCGGGAGGG GACGGTAATA CGGAATCGCT GGAGATTGTC 
AACAGGAAGG ATCGTCGGCA CCGGTCCGGA ATGAAGTCGA ACGGGTTTTT CGGGCCGGAA 
GGAAAGTACC AAACGAACAC CGACACTGTC AATGGTGGGA TCCCACGAAC GGAATCGTTT 
TCCCAACCAT CGTCAAATGG AACCAAAGTC AATTATAGTA CCATGAGCAA CTCCGCCTCC 
GCCATGGCGT TCTCAAACTC CTTAGATGAC TCCCATAGTG CCAATGCAAG TGAAACACCT 
ACCGGTGATC ACCCTTCCAA GTCGGTAGAT GAAATAATGC CTCTGCTCAA CCAAGCGAGC 
ATAACGTCGC TGGATGAGGA CGGCCTAGGT GCCGACGAAA GCGATAACAA CGAAGGACCA 
GTGACAGCCA AAGGACGTTC CCACCAATCC AGCCGGAAGG CTCATGGAAA TCGATCCGAA 
TCAGAGAACA GCCAGAGCGG TGAACGCGGA ATGATGTCGT TCAGTAGAAA AAAGCTAAAA 
TCCAAATACA TGAAGAGTAA GGCAGCGAAA GCAGGAATCG TTCTGGACGG TGCCAATGGG 
CAAATTACCA ATGCAGCAAA CGCTGCCAGT GATAGCGAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001653999.1
CDS	1..3645
Translation	MARSGGSKCSHLDNVVHLSLVELCKLKKGEPCTECDANGPNLWICLQKNCLHIGCSEQYNDHSTTHFQTNRTHCIHMNLSSQRIWCYLCECEVFLVQQQQQQQQQLRRTSVVSNDSSDASRYSANNNNEKLLMYGDRFASGFGGGESCDSSCEEDEFEKHGKWNGLVGLQNIANTCYMNSAIQALSNSPPLTGYFLDCGSIIETDLLGNQTVSKPGLAKHYHKLIKEMWCKNRRYVVPSGILYGIRIVHPMFRGYQQHDTQEFLRCFMDQLHEELKEVIQPPPELLSLHGRNDEDNEDHSPCPSPSPSQSEAEYETCDSGVSEQSSLSDDISVNNKRTRMISRSPSPTANQRSATSNSRQQSPIGSNRSNSSSSGSMSSMQQQQQAGSSDQQQQKPAKDKLPERSIISDIFDGKLLSSVQCLTCDRISTREETFQDLSLPIPGKDHLAVLHQNHPGMPSPSAHPSSPVAGSSSGITCSDAVYPVTGDSWMWWFWNWPFWSWVRSWFWGPAVTLHDCMAAFFSADELKGDNMYSCEKCNKLRNGVKFSRVLALPEMLCVHLKRFRHDLSYSSKISSPVNFPMNGLDMRPYLHKDCKSEVTSYDLGAVICHHGTVGGGHYTSFAKHEPTGKWFEFDDQLVTQVSPEDVQNCEAYVLFYRKNNSKMSTIRAQASDLISVQEHASDIRFYISRNWLHRFNTFAEPGPIDNWTLLCPHGAFPPHKAPYFSKLVVPIPQILWDFLYKKFGGGPVCNHIFPCHICKKAAESLSRRQRAELEEFTACNDEYQYNDTPTTIYAISMAWFRQWQLFARGVTTEEPGPIDNKTIAVPGDTNVPLRSVRQGSDYAQINSALWHFFHGIYGGGPEIVLRGNPVPPPEPKIPKVAKEREIEPMDVSEPTPPVKTTAPASVTQQHQHQKEHSAEATTVDPSPSTQSTTAKPQKSVSFEDNEGYHENDDDHNESSRSVQLKRKQLKQQSKESKKSESDSADGLVTYLRRKQLKHQQHVAASQGAGGDGNTESLEIVNRKDRRHRSGMKSNGFFGPEGKYQTNTDTVNGGIPRTESFSQPSSNGTKVNYSTMSNSASAMAFSNSLDDSHSANASETPTGDHPSKSVDEIMPLLNQASITSLDEDGLGADESDNNEGPVTAKGRSHQSSRKAHGNRSESENSQSGERGMMSFSRKKLKSKYMKSKAAKAGIVLDGANGQITNAANAASDSEK

Target ORF information:

RefSeq Version	XM_001653949.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL009702-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001653949.1

ORF Insert Sequence:

ATGGCTCGAA GTGGGGGCAG CAAGTGTTCT CATCTGGACA ATGTTGTTCA TCTCTCGTTG 
GTCGAGCTGT GCAAACTCAA GAAGGGTGAA CCATGTACGG AGTGCGACGC CAACGGTCCA 
AACCTGTGGA TATGTCTGCA GAAAAATTGT CTCCACATTG GCTGCTCGGA GCAGTACAAC 
GACCACAGTA CGACCCACTT CCAAACGAAC CGCACCCATT GCATCCACAT GAACCTGTCC 
TCTCAAAGGA TATGGTGCTA TTTGTGCGAG TGCGAAGTGT TCCTGGTGCA GCAACAACAA 
CAGCAACAGC AGCAGCTGAG ACGAACCTCG GTGGTGAGCA ACGATTCGAG TGATGCCAGT 
CGATACTCGG CCAACAACAA TAACGAGAAG CTGCTGATGT ATGGGGATCG GTTTGCCAGT 
GGATTTGGAG GTGGCGAATC CTGTGATAGC AGCTGCGAAG AAGACGAGTT TGAGAAGCAT 
GGTAAATGGA ATGGGCTGGT CGGGTTACAG AATATAGCAA ATACTTGCTA TATGAACTCC 
GCAATCCAAG CACTGAGCAA CAGTCCTCCC TTGACGGGAT ACTTCCTGGA TTGTGGGTCG 
ATCATAGAAA CCGATTTGCT CGGAAATCAA ACCGTTTCAA AGCCAGGTTT GGCCAAGCAT 
TACCACAAGT TGATCAAGGA AATGTGGTGT AAGAACAGGA GGTATGTCGT CCCCAGTGGA 
ATCCTGTATG GAATTCGAAT AGTACATCCC ATGTTTCGAG GCTATCAGCA ACATGACACC 
CAAGAATTTC TCCGCTGCTT CATGGATCAG CTACATGAAG AGTTGAAGGA AGTTATTCAA 
CCGCCGCCAG AGCTGCTATC ACTGCATGGT AGAAACGATG AAGACAATGA AGATCATTCG 
CCTTGTCCTT CGCCATCGCC TTCGCAATCC GAGGCAGAGT ATGAAACATG TGACAGCGGT 
GTTTCGGAGC AATCGAGCCT CTCGGATGAT ATATCAGTTA ATAATAAACG AACTAGAATG 
ATTTCGAGAT CTCCTAGCCC CACCGCAAAC CAAAGAAGTG CAACCTCGAA CAGTCGGCAA 
CAATCGCCGA TAGGAAGCAA TCGGAGCAAC TCCAGCAGCA GTGGAAGCAT GTCTTCTATG 
CAGCAGCAGC AGCAAGCCGG CTCCAGTGAT CAGCAACAAC AGAAACCTGC AAAGGACAAA 
CTTCCGGAAC GGTCCATCAT CAGCGACATA TTTGATGGGA AGCTTCTCTC ATCTGTACAA 
TGCTTAACCT GCGATCGTAT TTCAACTCGT GAAGAAACAT TTCAGGATCT ATCGTTACCA 
ATCCCCGGCA AAGATCATCT GGCAGTGTTG CATCAGAACC ACCCGGGAAT GCCTTCTCCT 
TCAGCTCATC CTTCGTCTCC GGTGGCTGGA AGTAGCAGTG GAATCACATG CTCTGATGCG 
GTCTATCCGG TAACCGGCGA TAGCTGGATG TGGTGGTTTT GGAACTGGCC TTTTTGGAGT 
TGGGTTCGAT CATGGTTCTG GGGACCAGCC GTTACACTGC ATGACTGCAT GGCAGCCTTC 
TTCAGTGCGG ACGAGCTGAA AGGAGACAAC ATGTACAGCT GCGAAAAGTG TAACAAACTC 
CGGAATGGGG TGAAGTTTTC CCGTGTTCTT GCCTTGCCTG AAATGTTGTG CGTCCATTTG 
AAACGTTTCC GGCACGATCT CTCCTACAGC TCGAAAATAT CCAGCCCCGT TAATTTCCCC 
ATGAACGGAC TAGACATGAG GCCATATCTG CACAAGGACT GCAAATCGGA AGTGACCTCG 
TACGATCTCG GTGCGGTCAT TTGTCACCAC GGCACGGTGG GAGGCGGACA TTATACCAGC 
TTTGCGAAAC ACGAACCCAC CGGGAAATGG TTCGAGTTCG ATGACCAGCT GGTGACGCAA 
GTCAGCCCGG AGGATGTCCA GAACTGTGAA GCTTATGTGC TGTTCTATCG CAAGAACAAC 
AGCAAAATGA GTACGATTCG GGCACAGGCC AGTGATCTGA TCAGCGTCCA GGAGCACGCT 
TCTGACATAA GGTTCTACAT TTCCCGGAAT TGGTTGCACA GGTTCAACAC GTTTGCTGAA 
CCCGGACCGA TAGACAACTG GACGTTGCTC TGTCCTCATG GCGCGTTTCC ACCACACAAA 
GCTCCGTACT TTTCGAAGCT GGTAGTGCCA ATCCCGCAGA TCTTGTGGGA CTTTCTGTAC 
AAAAAGTTCG GTGGAGGCCC GGTGTGCAAC CACATCTTCC CGTGTCATAT TTGCAAGAAG 
GCAGCCGAAT CGTTGTCCAG GAGGCAACGA GCCGAGCTGG AAGAATTCAC CGCCTGTAAT 
GACGAGTATC AGTACAATGA TACTCCAACC ACGATCTACG CGATCTCGAT GGCATGGTTC 
CGACAGTGGC AACTGTTTGC TCGCGGTGTC ACCACGGAAG AACCAGGACC TATCGATAAC 
AAAACCATTG CAGTTCCGGG GGATACAAAT GTTCCGCTTC GAAGCGTGCG CCAAGGATCG 
GACTACGCCC AGATCAACTC GGCCTTGTGG CACTTTTTCC ACGGCATCTA CGGTGGAGGG 
CCGGAAATCG TACTCCGCGG TAATCCGGTT CCGCCGCCAG AACCGAAAAT CCCGAAAGTG 
GCGAAGGAAC GAGAAATTGA ACCCATGGAC GTGAGTGAAC CAACTCCACC AGTGAAAACC 
ACTGCTCCGG CAAGCGTGAC CCAGCAGCAT CAGCATCAGA AGGAGCATTC CGCTGAAGCG 
ACCACAGTGG ATCCATCTCC AAGTACCCAA TCTACAACGG CGAAACCCCA GAAAAGTGTC 
TCCTTTGAGG ACAACGAAGG TTACCACGAA AACGACGACG ATCATAACGA ATCGTCTCGA 
TCAGTGCAGT TGAAGCGCAA GCAGCTGAAG CAGCAGTCGA AGGAATCGAA GAAATCCGAA 
TCCGACAGCG CCGATGGCCT GGTGACCTAT CTTCGGCGGA AGCAACTCAA GCACCAACAG 
CACGTGGCCG CTAGCCAAGG GGCGGGAGGG GACGGTAATA CGGAATCGCT GGAGATTGTC 
AACAGGAAGG ATCGTCGGCA CCGGTCCGGA ATGAAGTCGA ACGGGTTTTT CGGGCCGGAA 
GGAAAGTACC AAACGAACAC CGACACTGTC AATGGTGGGA TCCCACGAAC GGAATCGTTT 
TCCCAACCAT CGTCAAATGG AACCAAAGTC AATTATAGTA CCATGAGCAA CTCCGCCTCC 
GCCATGGCGT TCTCAAACTC CTTAGATGAC TCCCATAGTG CCAATGCAAG TGAAACACCT 
ACCGGTGATC ACCCTTCCAA GTCGGTAGAT GAAATAATGC CTCTGCTCAA CCAAGCGAGC 
ATAACGTCGC TGGATGAGGA CGGCCTAGGT GCCGACGAAA GCGATAACAA CGAAGGACCA 
GTGACAGCCA AAGGACGTTC CCACCAATCC AGCCGGAAGG CTCATGGAAA TCGATCCGAA 
TCAGAGAACA GCCAGAGCGG TGAACGCGGA ATGATGTCGT TCAGTAGAAA AAAGCTAAAA 
TCCAAATACA TGAAGAGTAA GGCAGCGAAA GCAGGAATCG TTCTGGACGG TGCCAATGGG 
CAAATTACCA ATGCAGCAAA CGCTGCCAGT GATAGCGAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5572305 ( XM_021854463.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021710155.1 Aedes aegypti ubiquitin carboxyl-terminal hydrolase 20 (LOC5572305), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe129332
Clone ID Related Accession (Same CDS sequence)	XM_021854463.1
Accession Version	XM_021854463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGAA GTGGGGGCAG CAAGTGTTCT CATCTGGACA ATGTTGTTCA TCTCTCGTTG 
GTCGAGCTGT GCAAGCTCAA GAAGGGTGAA CCATGTACGG AGTGCGACGC CAACGGTCCA 
AACCTGTGGA TATGTCTGCA GAAAAATTGT CTCCACATTG GCTGCTCGGA GCAGTACAAC 
GACCACAGTA CGACCCACTT CCAAACGAAC CGCACCCATT GCATCCACAT GAACCTGTCC 
TCTCAAAGGA TATGGTGCTA TTTGTGCGAG TGCGAAGTGT TCCTGGTGCA GCAACAACAA 
CAGCAACAGC AGCAGCTGAG ACGAACCTCG GTGGTGAGCA ACGATTCGAG TGATGCCAGT 
CGATACTCGG CCAACAACAA TAACGAGAAG CTGCTGATGT ATGGGGATCG GTTTGCCAGT 
GGATTTGGAG GTGGCGAATC CTGTGATAGC AGCTGCGAAG AAGACGAGTT TGAGAAGCAT 
GGTAAATGGA ATGGGCTGGT CGGGTTACAG AATATAGCAA ATACTTGCTA TATGAACTCG 
GCCATCCAAG CACTGAGCAA CAGTCCTCCT CTAACGGGAT ACTTCCTGGA TTGTGGGTCG 
ATCATAGAAA CCGATTTGCT CGGAAATCAA ACCGTTTCAA AGCCAGGTTT GGCCAAGCAT 
TACCACAAGT TGATAAAGGA AATGTGGTGT AAGAACAGAA GGTACGTTGT CCCCAGTGGA 
ATCCTGTATG GAATTCGAAT TGTACATCCT ATGTTCCGAG GCTACCAACA GCATGACACC 
CAAGAATTTC TTCGCTGCTT CATGGATCAG CTACATGAAG AGTTGAAGGA AGTTATTCAA 
CCACCGCCAG AGCTTTTATC ACTGCATGGC CGGAACGATG AAGACAATGA AGATCATTCA 
CCTTGTCCTT CGCCATCGCC TTCGCAATCC GAGGCAGAGT ATGAAACATG TGACAGCGGT 
GTTTCGGAGC AATCGAGCCT CTCGGATGAT ATATCAGTTA ATAATAAACG AACTAGAATG 
ATTTCGAGAT CTCCTAGCCC CACCGCAAAC CAAAGAAGTG CAACCTCGAA CAGTCGGCAA 
CAATCGCCGA TAGGAAGCAA TCGGAGCAAC TCCAGCAGCA GTGGAAGCAT GTCTTCTATG 
CAGCAGCAGC AGCAAGCCGG CTCCAGTGAT CAGCAACAAC AGAAACCTGC AAAGGACAAA 
CTTCCGGAAC GGTCCATCAT CAGCGACATA TTTGATGGGA AGCTTCTCTC ATCTGTACAA 
TGCTTAACCT GCGATCGTAT TTCAACTCGT GAAGAAACAT TTCAGGATCT ATCGTTACCA 
ATCCCCGGCA AAGATCATCT GGCAGTGTTG CATCAGAACC ACCCGGGAAT GCCTTCTCCT 
TCAGCTCATC CTTCGTCTCC GGTGGCTGGA AGTAGCAGTG GAATCACATG CTCTGATGCG 
GTCTATCCGG TAACCGGCGA TAGCTGGATG TGGTGGTTTT GGAACTGGCC TTTTTGGAGT 
TGGGTTCGAT CATGGTTCTG GGGACCAGCC GTTACACTGC ATGACTGCAT GGCAGCCTTC 
TTCAGCGCGG ACGAGCTGAA AGGAGACAAC ATGTACAGCT GCGAAAAGTG TAACAAACTC 
CGGAATGGGG TGAAGTTTTC CCGTGTTCTT GCCTTGCCTG AAATGTTGTG CGTCCATTTG 
AAACGTTTCC GGCACGATCT CTCCTACAGC TCGAAAATAT CCAGCCCCGT TAATTTCCCC 
ATGAACGGAC TAGACATGAG GCCATATCTG CACAAGGACT GCAAATCGGA AGTGACCTCG 
TACGATCTCT GTGCGGTCAT TTGTCACCAC GGCACGGTGG GAGGCGGACA TTATACCAGC 
TTTGCGAAAC ACGAACCCAC CGGGAAATGG TTCGAGTTCG ATGACCAGCT GGTGACGCAA 
GTCAGCCCGG AGGATGTCCA GAACTGTGAA GCTTATGTGC TGTTCTATCG CAAGAACAAC 
AGCAAAATGA GTACGATTCG GGCACAGGCC AGTGATCTGA TCAGCGTCCA GGAGCACGCT 
TCTGACATAA GGTTCTACAT TTCCCGGAAT TGGTTGCACA GATTCAACAC GTTTGCTGAA 
CCCGGACCGA TAGACAACTG GACGTTGCTC TGTCCCCATG GCGCGTTTCC ACCACACAAA 
GCTCCGTACT TTTCGAAGCT GGTAGTGCCA ATCCCGCAGA TCTTGTGGGA CTTTCTGTAC 
AAAAAGTTCG GTGGAGGCCC GGTGTGCAAT CACATCTTCC CGTGTCATAT TTGCAAGAAG 
GCAGCCGAAT CGTTGTCCAG GAGGCAACGA GCCGAGCTGG AAGAATTCAC CGCCTGTAAT 
GACGAGTATC AGCAGTACAA TGATACTCCA ACCACGATCT ACGCGATCTC GATGGCATGG 
TTCCGACAGT GGCAACTGTT TGCTCGCGGT GTCACCACGG AAGAACCAGG ACCTATCGAT 
AACAAAACCA TTGCAGTTCC GGGGGATACA AATGTTCCGC TTCGAAGCGT GCGCCAAGGA 
TCGGACTACG CCCAGATCAA CTCGGCCTTG TGGCACTTTT TCCACGGCAT CTACGGTGGA 
GGGCCGGAAA TCGTACTCCG CGGTAATCCG GTTCCGCCGC CAGAACCGAA AATCCCGAAA 
GTGGCTAAGG AACGAGAAAT TGAACCCATG GACGTGAGTG AACCAACTCC ACCAGTGAAA 
ACCACTGCTC CGGCAAGCGT GACCCAGCAG CATCAGCATC AGAAGGAGCA TTCCGCTGAA 
GCGACCACAG TGGATCCATC TCCAAGTACC CAATCTACAA CGGCGAAACC CCAGAAAAGT 
GTCTCCTTTG AGGACAACGA AGGTTACCAC GAAAACGACG ACGATCATAA CGAATCGTCT 
CGATCAGTGC AGTTGAAGCG CAAGCAGCTG AAGCAGCAGT CGAAGGAATC GAAGAAATCC 
GAATCCGACA GCGCCGATGG ACTGGTGACC TATCTTCGGC GGAAGCAACT CAAGCACCAA 
CAGCACGTGG CCACTAGCCA AGGGGCGGGA GGGGACGGTA ATACGGAATC GCTGGAGATT 
GTCAACAGGA AGGATCGTCG GCACCGGTCC GGAATGAAGT CGAACGGGTT TTTCGGGCCG 
GAAGGAAAGT ACCAAACGAA CACCGACACT GTCAATGGTG GGATCCCACG AACGGAATCG 
TTTTCCCAAC CATCGTCAAA TGGAACCAAA GTCAATTATA GTACCATGAG CAACTCCGTC 
TCCGCCATGG CGTTCTCAAA CTCCTTAGAT GACTCCCATA GTGCCAATGC AAGTGAAACA 
CCCGCCGGTG GTCACCCTTC CAAGTCGGTA GATGAAATAA TGCCTCTGCT CAACCAAGCG 
AGCATAACGA GCACGTCGCT GGATGAGGAC GGCCTAGGTG CCGACGAAAG CGATAACAAC 
GAAGGACCAG TGACAGCCAA AGGACGTTCC CACCAATCCA GCCGGAAGGC TCATGGAAAT 
CGATCCGAGT CGGAGAACAG CCAGAGCGGT GAACGCGGAA TGATGTCGTT CAGTAGAAAG 
AAGCTAAAAT CCAAATACAT GAAGAGTAAG GCAGCGAAAG CTGGGATCGT TCTGGACGGT 
GCCAATGGGC AAATTACCAA TGCAGCAAAC GCTGCCAGTG ATAGCGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021710155.1
CDS	1001..4654
Translation	MARSGGSKCSHLDNVVHLSLVELCKLKKGEPCTECDANGPNLWICLQKNCLHIGCSEQYNDHSTTHFQTNRTHCIHMNLSSQRIWCYLCECEVFLVQQQQQQQQQLRRTSVVSNDSSDASRYSANNNNEKLLMYGDRFASGFGGGESCDSSCEEDEFEKHGKWNGLVGLQNIANTCYMNSAIQALSNSPPLTGYFLDCGSIIETDLLGNQTVSKPGLAKHYHKLIKEMWCKNRRYVVPSGILYGIRIVHPMFRGYQQHDTQEFLRCFMDQLHEELKEVIQPPPELLSLHGRNDEDNEDHSPCPSPSPSQSEAEYETCDSGVSEQSSLSDDISVNNKRTRMISRSPSPTANQRSATSNSRQQSPIGSNRSNSSSSGSMSSMQQQQQAGSSDQQQQKPAKDKLPERSIISDIFDGKLLSSVQCLTCDRISTREETFQDLSLPIPGKDHLAVLHQNHPGMPSPSAHPSSPVAGSSSGITCSDAVYPVTGDSWMWWFWNWPFWSWVRSWFWGPAVTLHDCMAAFFSADELKGDNMYSCEKCNKLRNGVKFSRVLALPEMLCVHLKRFRHDLSYSSKISSPVNFPMNGLDMRPYLHKDCKSEVTSYDLCAVICHHGTVGGGHYTSFAKHEPTGKWFEFDDQLVTQVSPEDVQNCEAYVLFYRKNNSKMSTIRAQASDLISVQEHASDIRFYISRNWLHRFNTFAEPGPIDNWTLLCPHGAFPPHKAPYFSKLVVPIPQILWDFLYKKFGGGPVCNHIFPCHICKKAAESLSRRQRAELEEFTACNDEYQQYNDTPTTIYAISMAWFRQWQLFARGVTTEEPGPIDNKTIAVPGDTNVPLRSVRQGSDYAQINSALWHFFHGIYGGGPEIVLRGNPVPPPEPKIPKVAKEREIEPMDVSEPTPPVKTTAPASVTQQHQHQKEHSAEATTVDPSPSTQSTTAKPQKSVSFEDNEGYHENDDDHNESSRSVQLKRKQLKQQSKESKKSESDSADGLVTYLRRKQLKHQQHVATSQGAGGDGNTESLEIVNRKDRRHRSGMKSNGFFGPEGKYQTNTDTVNGGIPRTESFSQPSSNGTKVNYSTMSNSVSAMAFSNSLDDSHSANASETPAGGHPSKSVDEIMPLLNQASITSTSLDEDGLGADESDNNEGPVTAKGRSHQSSRKAHGNRSESENSQSGERGMMSFSRKKLKSKYMKSKAAKAGIVLDGANGQITNAANAASDSEK

Target ORF information:

RefSeq Version	XM_021854463.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti ubiquitin carboxyl-terminal hydrolase 20 (LOC5572305), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021854463.1

ORF Insert Sequence:

ATGGCTCGAA GTGGGGGCAG CAAGTGTTCT CATCTGGACA ATGTTGTTCA TCTCTCGTTG 
GTCGAGCTGT GCAAGCTCAA GAAGGGTGAA CCATGTACGG AGTGCGACGC CAACGGTCCA 
AACCTGTGGA TATGTCTGCA GAAAAATTGT CTCCACATTG GCTGCTCGGA GCAGTACAAC 
GACCACAGTA CGACCCACTT CCAAACGAAC CGCACCCATT GCATCCACAT GAACCTGTCC 
TCTCAAAGGA TATGGTGCTA TTTGTGCGAG TGCGAAGTGT TCCTGGTGCA GCAACAACAA 
CAGCAACAGC AGCAGCTGAG ACGAACCTCG GTGGTGAGCA ACGATTCGAG TGATGCCAGT 
CGATACTCGG CCAACAACAA TAACGAGAAG CTGCTGATGT ATGGGGATCG GTTTGCCAGT 
GGATTTGGAG GTGGCGAATC CTGTGATAGC AGCTGCGAAG AAGACGAGTT TGAGAAGCAT 
GGTAAATGGA ATGGGCTGGT CGGGTTACAG AATATAGCAA ATACTTGCTA TATGAACTCG 
GCCATCCAAG CACTGAGCAA CAGTCCTCCT CTAACGGGAT ACTTCCTGGA TTGTGGGTCG 
ATCATAGAAA CCGATTTGCT CGGAAATCAA ACCGTTTCAA AGCCAGGTTT GGCCAAGCAT 
TACCACAAGT TGATAAAGGA AATGTGGTGT AAGAACAGAA GGTACGTTGT CCCCAGTGGA 
ATCCTGTATG GAATTCGAAT TGTACATCCT ATGTTCCGAG GCTACCAACA GCATGACACC 
CAAGAATTTC TTCGCTGCTT CATGGATCAG CTACATGAAG AGTTGAAGGA AGTTATTCAA 
CCACCGCCAG AGCTTTTATC ACTGCATGGC CGGAACGATG AAGACAATGA AGATCATTCA 
CCTTGTCCTT CGCCATCGCC TTCGCAATCC GAGGCAGAGT ATGAAACATG TGACAGCGGT 
GTTTCGGAGC AATCGAGCCT CTCGGATGAT ATATCAGTTA ATAATAAACG AACTAGAATG 
ATTTCGAGAT CTCCTAGCCC CACCGCAAAC CAAAGAAGTG CAACCTCGAA CAGTCGGCAA 
CAATCGCCGA TAGGAAGCAA TCGGAGCAAC TCCAGCAGCA GTGGAAGCAT GTCTTCTATG 
CAGCAGCAGC AGCAAGCCGG CTCCAGTGAT CAGCAACAAC AGAAACCTGC AAAGGACAAA 
CTTCCGGAAC GGTCCATCAT CAGCGACATA TTTGATGGGA AGCTTCTCTC ATCTGTACAA 
TGCTTAACCT GCGATCGTAT TTCAACTCGT GAAGAAACAT TTCAGGATCT ATCGTTACCA 
ATCCCCGGCA AAGATCATCT GGCAGTGTTG CATCAGAACC ACCCGGGAAT GCCTTCTCCT 
TCAGCTCATC CTTCGTCTCC GGTGGCTGGA AGTAGCAGTG GAATCACATG CTCTGATGCG 
GTCTATCCGG TAACCGGCGA TAGCTGGATG TGGTGGTTTT GGAACTGGCC TTTTTGGAGT 
TGGGTTCGAT CATGGTTCTG GGGACCAGCC GTTACACTGC ATGACTGCAT GGCAGCCTTC 
TTCAGCGCGG ACGAGCTGAA AGGAGACAAC ATGTACAGCT GCGAAAAGTG TAACAAACTC 
CGGAATGGGG TGAAGTTTTC CCGTGTTCTT GCCTTGCCTG AAATGTTGTG CGTCCATTTG 
AAACGTTTCC GGCACGATCT CTCCTACAGC TCGAAAATAT CCAGCCCCGT TAATTTCCCC 
ATGAACGGAC TAGACATGAG GCCATATCTG CACAAGGACT GCAAATCGGA AGTGACCTCG 
TACGATCTCT GTGCGGTCAT TTGTCACCAC GGCACGGTGG GAGGCGGACA TTATACCAGC 
TTTGCGAAAC ACGAACCCAC CGGGAAATGG TTCGAGTTCG ATGACCAGCT GGTGACGCAA 
GTCAGCCCGG AGGATGTCCA GAACTGTGAA GCTTATGTGC TGTTCTATCG CAAGAACAAC 
AGCAAAATGA GTACGATTCG GGCACAGGCC AGTGATCTGA TCAGCGTCCA GGAGCACGCT 
TCTGACATAA GGTTCTACAT TTCCCGGAAT TGGTTGCACA GATTCAACAC GTTTGCTGAA 
CCCGGACCGA TAGACAACTG GACGTTGCTC TGTCCCCATG GCGCGTTTCC ACCACACAAA 
GCTCCGTACT TTTCGAAGCT GGTAGTGCCA ATCCCGCAGA TCTTGTGGGA CTTTCTGTAC 
AAAAAGTTCG GTGGAGGCCC GGTGTGCAAT CACATCTTCC CGTGTCATAT TTGCAAGAAG 
GCAGCCGAAT CGTTGTCCAG GAGGCAACGA GCCGAGCTGG AAGAATTCAC CGCCTGTAAT 
GACGAGTATC AGCAGTACAA TGATACTCCA ACCACGATCT ACGCGATCTC GATGGCATGG 
TTCCGACAGT GGCAACTGTT TGCTCGCGGT GTCACCACGG AAGAACCAGG ACCTATCGAT 
AACAAAACCA TTGCAGTTCC GGGGGATACA AATGTTCCGC TTCGAAGCGT GCGCCAAGGA 
TCGGACTACG CCCAGATCAA CTCGGCCTTG TGGCACTTTT TCCACGGCAT CTACGGTGGA 
GGGCCGGAAA TCGTACTCCG CGGTAATCCG GTTCCGCCGC CAGAACCGAA AATCCCGAAA 
GTGGCTAAGG AACGAGAAAT TGAACCCATG GACGTGAGTG AACCAACTCC ACCAGTGAAA 
ACCACTGCTC CGGCAAGCGT GACCCAGCAG CATCAGCATC AGAAGGAGCA TTCCGCTGAA 
GCGACCACAG TGGATCCATC TCCAAGTACC CAATCTACAA CGGCGAAACC CCAGAAAAGT 
GTCTCCTTTG AGGACAACGA AGGTTACCAC GAAAACGACG ACGATCATAA CGAATCGTCT 
CGATCAGTGC AGTTGAAGCG CAAGCAGCTG AAGCAGCAGT CGAAGGAATC GAAGAAATCC 
GAATCCGACA GCGCCGATGG ACTGGTGACC TATCTTCGGC GGAAGCAACT CAAGCACCAA 
CAGCACGTGG CCACTAGCCA AGGGGCGGGA GGGGACGGTA ATACGGAATC GCTGGAGATT 
GTCAACAGGA AGGATCGTCG GCACCGGTCC GGAATGAAGT CGAACGGGTT TTTCGGGCCG 
GAAGGAAAGT ACCAAACGAA CACCGACACT GTCAATGGTG GGATCCCACG AACGGAATCG 
TTTTCCCAAC CATCGTCAAA TGGAACCAAA GTCAATTATA GTACCATGAG CAACTCCGTC 
TCCGCCATGG CGTTCTCAAA CTCCTTAGAT GACTCCCATA GTGCCAATGC AAGTGAAACA 
CCCGCCGGTG GTCACCCTTC CAAGTCGGTA GATGAAATAA TGCCTCTGCT CAACCAAGCG 
AGCATAACGA GCACGTCGCT GGATGAGGAC GGCCTAGGTG CCGACGAAAG CGATAACAAC 
GAAGGACCAG TGACAGCCAA AGGACGTTCC CACCAATCCA GCCGGAAGGC TCATGGAAAT 
CGATCCGAGT CGGAGAACAG CCAGAGCGGT GAACGCGGAA TGATGTCGTT CAGTAGAAAG 
AAGCTAAAAT CCAAATACAT GAAGAGTAAG GCAGCGAAAG CTGGGATCGT TCTGGACGGT 
GCCAATGGGC AAATTACCAA TGCAGCAAAC GCTGCCAGTG ATAGCGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5572305 ( XM_001653949.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001653999.2 Aedes aegypti ubiquitin carboxyl-terminal hydrolase 20 (LOC5572305), transcript variant X2, mRNA.

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CloneID	OAe129333
Clone ID Related Accession (Same CDS sequence)	XM_001653949.2
Accession Version	XM_001653949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001653949.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGAA GTGGGGGCAG CAAGTGTTCT CATCTGGACA ATGTTGTTCA TCTCTCGTTG 
GTCGAGCTGT GCAAGCTCAA GAAGGGTGAA CCATGTACGG AGTGCGACGC CAACGGTCCA 
AACCTGTGGA TATGTCTGCA GAAAAATTGT CTCCACATTG GCTGCTCGGA GCAGTACAAC 
GACCACAGTA CGACCCACTT CCAAACGAAC CGCACCCATT GCATCCACAT GAACCTGTCC 
TCTCAAAGGA TATGGTGCTA TTTGTGCGAG TGCGAAGTGT TCCTGGTGCA GCAACAACAA 
CAGCAACAGC AGCAGCTGAG ACGAACCTCG GTGGTGAGCA ACGATTCGAG TGATGCCAGT 
CGATACTCGG CCAACAACAA TAACGAGAAG CTGCTGATGT ATGGGGATCG GTTTGCCAGT 
GGATTTGGAG GTGGCGAATC CTGTGATAGC AGCTGCGAAG AAGACGAGTT TGAGAAGCAT 
GGTAAATGGA ATGGGCTGGT CGGGTTACAG AATATAGCAA ATACTTGCTA TATGAACTCG 
GCCATCCAAG CACTGAGCAA CAGTCCTCCT CTAACGGGAT ACTTCCTGGA TTGTGGGTCG 
ATCATAGAAA CCGATTTGCT CGGAAATCAA ACCGTTTCAA AGCCAGGTTT GGCCAAGCAT 
TACCACAAGT TGATAAAGGA AATGTGGTGT AAGAACAGAA GGTACGTTGT CCCCAGTGGA 
ATCCTGTATG GAATTCGAAT TGTACATCCT ATGTTCCGAG GCTACCAACA GCATGACACC 
CAAGAATTTC TTCGCTGCTT CATGGATCAG CTACATGAAG AGTTGAAGGA AGTTATTCAA 
CCACCGCCAG AGCTTTTATC ACTGCATGGC CGGAACGATG AAGACAATGA AGATCATTCA 
CCTTGTCCTT CGCCATCGCC TTCGCAATCC GAGGCAGAGT ATGAAACATG TGACAGCGGT 
GTTTCGGAGC AATCGAGCCT CTCGGATGAT ATATCAGTTA ATAATAAACG AACTAGAATG 
ATTTCGAGAT CTCCTAGCCC CACCGCAAAC CAAAGAAGTG CAACCTCGAA CAGTCGGCAA 
CAATCGCCGA TAGGAAGCAA TCGGAGCAAC TCCAGCAGCA GTGGAAGCAT GTCTTCTATG 
CAGCAGCAGC AGCAAGCCGG CTCCAGTGAT CAGCAACAAC AGAAACCTGC AAAGGACAAA 
CTTCCGGAAC GGTCCATCAT CAGCGACATA TTTGATGGGA AGCTTCTCTC ATCTGTACAA 
TGCTTAACCT GCGATCGTAT TTCAACTCGT GAAGAAACAT TTCAGGATCT ATCGTTACCA 
ATCCCCGGCA AAGATCATCT GGCAGTGTTG CATCAGAACC ACCCGGGAAT GCCTTCTCCT 
TCAGCTCATC CTTCGTCTCC GGTGGCTGGA AGTAGCAGTG GAATCACATG CTCTGATGCG 
GTCTATCCGG TAACCGGCGA TAGCTGGATG TGGTGGTTTT GGAACTGGCC TTTTTGGAGT 
TGGGTTCGAT CATGGTTCTG GGGACCAGCC GTTACACTGC ATGACTGCAT GGCAGCCTTC 
TTCAGCGCGG ACGAGCTGAA AGGAGACAAC ATGTACAGCT GCGAAAAGTG TAACAAACTC 
CGGAATGGGG TGAAGTTTTC CCGTGTTCTT GCCTTGCCTG AAATGTTGTG CGTCCATTTG 
AAACGTTTCC GGCACGATCT CTCCTACAGC TCGAAAATAT CCAGCCCCGT TAATTTCCCC 
ATGAACGGAC TAGACATGAG GCCATATCTG CACAAGGACT GCAAATCGGA AGTGACCTCG 
TACGATCTCT GTGCGGTCAT TTGTCACCAC GGCACGGTGG GAGGCGGACA TTATACCAGC 
TTTGCGAAAC ACGAACCCAC CGGGAAATGG TTCGAGTTCG ATGACCAGCT GGTGACGCAA 
GTCAGCCCGG AGGATGTCCA GAACTGTGAA GCTTATGTGC TGTTCTATCG CAAGAACAAC 
AGCAAAATGA GTACGATTCG GGCACAGGCC AGTGATCTGA TCAGCGTCCA GGAGCACGCT 
TCTGACATAA GGTTCTACAT TTCCCGGAAT TGGTTGCACA GATTCAACAC GTTTGCTGAA 
CCCGGACCGA TAGACAACTG GACGTTGCTC TGTCCCCATG GCGCGTTTCC ACCACACAAA 
GCTCCGTACT TTTCGAAGCT GGTAGTGCCA ATCCCGCAGA TCTTGTGGGA CTTTCTGTAC 
AAAAAGTTCG GTGGAGGCCC GGTGTGCAAT CACATCTTCC CGTGTCATAT TTGCAAGAAG 
GCAGCCGAAT CGTTGTCCAG GAGGCAACGA GCCGAGCTGG AAGAATTCAC CGCCTGTAAT 
GACGAGTATC AGTACAATGA TACTCCAACC ACGATCTACG CGATCTCGAT GGCATGGTTC 
CGACAGTGGC AACTGTTTGC TCGCGGTGTC ACCACGGAAG AACCAGGACC TATCGATAAC 
AAAACCATTG CAGTTCCGGG GGATACAAAT GTTCCGCTTC GAAGCGTGCG CCAAGGATCG 
GACTACGCCC AGATCAACTC GGCCTTGTGG CACTTTTTCC ACGGCATCTA CGGTGGAGGG 
CCGGAAATCG TACTCCGCGG TAATCCGGTT CCGCCGCCAG AACCGAAAAT CCCGAAAGTG 
GCTAAGGAAC GAGAAATTGA ACCCATGGAC GTGAGTGAAC CAACTCCACC AGTGAAAACC 
ACTGCTCCGG CAAGCGTGAC CCAGCAGCAT CAGCATCAGA AGGAGCATTC CGCTGAAGCG 
ACCACAGTGG ATCCATCTCC AAGTACCCAA TCTACAACGG CGAAACCCCA GAAAAGTGTC 
TCCTTTGAGG ACAACGAAGG TTACCACGAA AACGACGACG ATCATAACGA ATCGTCTCGA 
TCAGTGCAGT TGAAGCGCAA GCAGCTGAAG CAGCAGTCGA AGGAATCGAA GAAATCCGAA 
TCCGACAGCG CCGATGGACT GGTGACCTAT CTTCGGCGGA AGCAACTCAA GCACCAACAG 
CACGTGGCCA CTAGCCAAGG GGCGGGAGGG GACGGTAATA CGGAATCGCT GGAGATTGTC 
AACAGGAAGG ATCGTCGGCA CCGGTCCGGA ATGAAGTCGA ACGGGTTTTT CGGGCCGGAA 
GGAAAGTACC AAACGAACAC CGACACTGTC AATGGTGGGA TCCCACGAAC GGAATCGTTT 
TCCCAACCAT CGTCAAATGG AACCAAAGTC AATTATAGTA CCATGAGCAA CTCCGTCTCC 
GCCATGGCGT TCTCAAACTC CTTAGATGAC TCCCATAGTG CCAATGCAAG TGAAACACCC 
GCCGGTGGTC ACCCTTCCAA GTCGGTAGAT GAAATAATGC CTCTGCTCAA CCAAGCGAGC 
ATAACGAGCA CGTCGCTGGA TGAGGACGGC CTAGGTGCCG ACGAAAGCGA TAACAACGAA 
GGACCAGTGA CAGCCAAAGG ACGTTCCCAC CAATCCAGCC GGAAGGCTCA TGGAAATCGA 
TCCGAGTCGG AGAACAGCCA GAGCGGTGAA CGCGGAATGA TGTCGTTCAG TAGAAAGAAG 
CTAAAATCCA AATACATGAA GAGTAAGGCA GCGAAAGCTG GGATCGTTCT GGACGGTGCC 
AATGGGCAAA TTACCAATGC AGCAAACGCT GCCAGTGATA GCGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001653999.2
CDS	1001..4651
Translation	MARSGGSKCSHLDNVVHLSLVELCKLKKGEPCTECDANGPNLWICLQKNCLHIGCSEQYNDHSTTHFQTNRTHCIHMNLSSQRIWCYLCECEVFLVQQQQQQQQQLRRTSVVSNDSSDASRYSANNNNEKLLMYGDRFASGFGGGESCDSSCEEDEFEKHGKWNGLVGLQNIANTCYMNSAIQALSNSPPLTGYFLDCGSIIETDLLGNQTVSKPGLAKHYHKLIKEMWCKNRRYVVPSGILYGIRIVHPMFRGYQQHDTQEFLRCFMDQLHEELKEVIQPPPELLSLHGRNDEDNEDHSPCPSPSPSQSEAEYETCDSGVSEQSSLSDDISVNNKRTRMISRSPSPTANQRSATSNSRQQSPIGSNRSNSSSSGSMSSMQQQQQAGSSDQQQQKPAKDKLPERSIISDIFDGKLLSSVQCLTCDRISTREETFQDLSLPIPGKDHLAVLHQNHPGMPSPSAHPSSPVAGSSSGITCSDAVYPVTGDSWMWWFWNWPFWSWVRSWFWGPAVTLHDCMAAFFSADELKGDNMYSCEKCNKLRNGVKFSRVLALPEMLCVHLKRFRHDLSYSSKISSPVNFPMNGLDMRPYLHKDCKSEVTSYDLCAVICHHGTVGGGHYTSFAKHEPTGKWFEFDDQLVTQVSPEDVQNCEAYVLFYRKNNSKMSTIRAQASDLISVQEHASDIRFYISRNWLHRFNTFAEPGPIDNWTLLCPHGAFPPHKAPYFSKLVVPIPQILWDFLYKKFGGGPVCNHIFPCHICKKAAESLSRRQRAELEEFTACNDEYQYNDTPTTIYAISMAWFRQWQLFARGVTTEEPGPIDNKTIAVPGDTNVPLRSVRQGSDYAQINSALWHFFHGIYGGGPEIVLRGNPVPPPEPKIPKVAKEREIEPMDVSEPTPPVKTTAPASVTQQHQHQKEHSAEATTVDPSPSTQSTTAKPQKSVSFEDNEGYHENDDDHNESSRSVQLKRKQLKQQSKESKKSESDSADGLVTYLRRKQLKHQQHVATSQGAGGDGNTESLEIVNRKDRRHRSGMKSNGFFGPEGKYQTNTDTVNGGIPRTESFSQPSSNGTKVNYSTMSNSVSAMAFSNSLDDSHSANASETPAGGHPSKSVDEIMPLLNQASITSTSLDEDGLGADESDNNEGPVTAKGRSHQSSRKAHGNRSESENSQSGERGMMSFSRKKLKSKYMKSKAAKAGIVLDGANGQITNAANAASDSEK

Target ORF information:

RefSeq Version	XM_001653949.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti ubiquitin carboxyl-terminal hydrolase 20 (LOC5572305), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001653949.2

ORF Insert Sequence:

ATGGCTCGAA GTGGGGGCAG CAAGTGTTCT CATCTGGACA ATGTTGTTCA TCTCTCGTTG 
GTCGAGCTGT GCAAGCTCAA GAAGGGTGAA CCATGTACGG AGTGCGACGC CAACGGTCCA 
AACCTGTGGA TATGTCTGCA GAAAAATTGT CTCCACATTG GCTGCTCGGA GCAGTACAAC 
GACCACAGTA CGACCCACTT CCAAACGAAC CGCACCCATT GCATCCACAT GAACCTGTCC 
TCTCAAAGGA TATGGTGCTA TTTGTGCGAG TGCGAAGTGT TCCTGGTGCA GCAACAACAA 
CAGCAACAGC AGCAGCTGAG ACGAACCTCG GTGGTGAGCA ACGATTCGAG TGATGCCAGT 
CGATACTCGG CCAACAACAA TAACGAGAAG CTGCTGATGT ATGGGGATCG GTTTGCCAGT 
GGATTTGGAG GTGGCGAATC CTGTGATAGC AGCTGCGAAG AAGACGAGTT TGAGAAGCAT 
GGTAAATGGA ATGGGCTGGT CGGGTTACAG AATATAGCAA ATACTTGCTA TATGAACTCG 
GCCATCCAAG CACTGAGCAA CAGTCCTCCT CTAACGGGAT ACTTCCTGGA TTGTGGGTCG 
ATCATAGAAA CCGATTTGCT CGGAAATCAA ACCGTTTCAA AGCCAGGTTT GGCCAAGCAT 
TACCACAAGT TGATAAAGGA AATGTGGTGT AAGAACAGAA GGTACGTTGT CCCCAGTGGA 
ATCCTGTATG GAATTCGAAT TGTACATCCT ATGTTCCGAG GCTACCAACA GCATGACACC 
CAAGAATTTC TTCGCTGCTT CATGGATCAG CTACATGAAG AGTTGAAGGA AGTTATTCAA 
CCACCGCCAG AGCTTTTATC ACTGCATGGC CGGAACGATG AAGACAATGA AGATCATTCA 
CCTTGTCCTT CGCCATCGCC TTCGCAATCC GAGGCAGAGT ATGAAACATG TGACAGCGGT 
GTTTCGGAGC AATCGAGCCT CTCGGATGAT ATATCAGTTA ATAATAAACG AACTAGAATG 
ATTTCGAGAT CTCCTAGCCC CACCGCAAAC CAAAGAAGTG CAACCTCGAA CAGTCGGCAA 
CAATCGCCGA TAGGAAGCAA TCGGAGCAAC TCCAGCAGCA GTGGAAGCAT GTCTTCTATG 
CAGCAGCAGC AGCAAGCCGG CTCCAGTGAT CAGCAACAAC AGAAACCTGC AAAGGACAAA 
CTTCCGGAAC GGTCCATCAT CAGCGACATA TTTGATGGGA AGCTTCTCTC ATCTGTACAA 
TGCTTAACCT GCGATCGTAT TTCAACTCGT GAAGAAACAT TTCAGGATCT ATCGTTACCA 
ATCCCCGGCA AAGATCATCT GGCAGTGTTG CATCAGAACC ACCCGGGAAT GCCTTCTCCT 
TCAGCTCATC CTTCGTCTCC GGTGGCTGGA AGTAGCAGTG GAATCACATG CTCTGATGCG 
GTCTATCCGG TAACCGGCGA TAGCTGGATG TGGTGGTTTT GGAACTGGCC TTTTTGGAGT 
TGGGTTCGAT CATGGTTCTG GGGACCAGCC GTTACACTGC ATGACTGCAT GGCAGCCTTC 
TTCAGCGCGG ACGAGCTGAA AGGAGACAAC ATGTACAGCT GCGAAAAGTG TAACAAACTC 
CGGAATGGGG TGAAGTTTTC CCGTGTTCTT GCCTTGCCTG AAATGTTGTG CGTCCATTTG 
AAACGTTTCC GGCACGATCT CTCCTACAGC TCGAAAATAT CCAGCCCCGT TAATTTCCCC 
ATGAACGGAC TAGACATGAG GCCATATCTG CACAAGGACT GCAAATCGGA AGTGACCTCG 
TACGATCTCT GTGCGGTCAT TTGTCACCAC GGCACGGTGG GAGGCGGACA TTATACCAGC 
TTTGCGAAAC ACGAACCCAC CGGGAAATGG TTCGAGTTCG ATGACCAGCT GGTGACGCAA 
GTCAGCCCGG AGGATGTCCA GAACTGTGAA GCTTATGTGC TGTTCTATCG CAAGAACAAC 
AGCAAAATGA GTACGATTCG GGCACAGGCC AGTGATCTGA TCAGCGTCCA GGAGCACGCT 
TCTGACATAA GGTTCTACAT TTCCCGGAAT TGGTTGCACA GATTCAACAC GTTTGCTGAA 
CCCGGACCGA TAGACAACTG GACGTTGCTC TGTCCCCATG GCGCGTTTCC ACCACACAAA 
GCTCCGTACT TTTCGAAGCT GGTAGTGCCA ATCCCGCAGA TCTTGTGGGA CTTTCTGTAC 
AAAAAGTTCG GTGGAGGCCC GGTGTGCAAT CACATCTTCC CGTGTCATAT TTGCAAGAAG 
GCAGCCGAAT CGTTGTCCAG GAGGCAACGA GCCGAGCTGG AAGAATTCAC CGCCTGTAAT 
GACGAGTATC AGTACAATGA TACTCCAACC ACGATCTACG CGATCTCGAT GGCATGGTTC 
CGACAGTGGC AACTGTTTGC TCGCGGTGTC ACCACGGAAG AACCAGGACC TATCGATAAC 
AAAACCATTG CAGTTCCGGG GGATACAAAT GTTCCGCTTC GAAGCGTGCG CCAAGGATCG 
GACTACGCCC AGATCAACTC GGCCTTGTGG CACTTTTTCC ACGGCATCTA CGGTGGAGGG 
CCGGAAATCG TACTCCGCGG TAATCCGGTT CCGCCGCCAG AACCGAAAAT CCCGAAAGTG 
GCTAAGGAAC GAGAAATTGA ACCCATGGAC GTGAGTGAAC CAACTCCACC AGTGAAAACC 
ACTGCTCCGG CAAGCGTGAC CCAGCAGCAT CAGCATCAGA AGGAGCATTC CGCTGAAGCG 
ACCACAGTGG ATCCATCTCC AAGTACCCAA TCTACAACGG CGAAACCCCA GAAAAGTGTC 
TCCTTTGAGG ACAACGAAGG TTACCACGAA AACGACGACG ATCATAACGA ATCGTCTCGA 
TCAGTGCAGT TGAAGCGCAA GCAGCTGAAG CAGCAGTCGA AGGAATCGAA GAAATCCGAA 
TCCGACAGCG CCGATGGACT GGTGACCTAT CTTCGGCGGA AGCAACTCAA GCACCAACAG 
CACGTGGCCA CTAGCCAAGG GGCGGGAGGG GACGGTAATA CGGAATCGCT GGAGATTGTC 
AACAGGAAGG ATCGTCGGCA CCGGTCCGGA ATGAAGTCGA ACGGGTTTTT CGGGCCGGAA 
GGAAAGTACC AAACGAACAC CGACACTGTC AATGGTGGGA TCCCACGAAC GGAATCGTTT 
TCCCAACCAT CGTCAAATGG AACCAAAGTC AATTATAGTA CCATGAGCAA CTCCGTCTCC 
GCCATGGCGT TCTCAAACTC CTTAGATGAC TCCCATAGTG CCAATGCAAG TGAAACACCC 
GCCGGTGGTC ACCCTTCCAA GTCGGTAGAT GAAATAATGC CTCTGCTCAA CCAAGCGAGC 
ATAACGAGCA CGTCGCTGGA TGAGGACGGC CTAGGTGCCG ACGAAAGCGA TAACAACGAA 
GGACCAGTGA CAGCCAAAGG ACGTTCCCAC CAATCCAGCC GGAAGGCTCA TGGAAATCGA 
TCCGAGTCGG AGAACAGCCA GAGCGGTGAA CGCGGAATGA TGTCGTTCAG TAGAAAGAAG 
CTAAAATCCA AATACATGAA GAGTAAGGCA GCGAAAGCTG GGATCGTTCT GGACGGTGCC 
AATGGGCAAA TTACCAATGC AGCAAACGCT GCCAGTGATA GCGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5572305 ( XM_021854464.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021710156.1 Aedes aegypti ubiquitin carboxyl-terminal hydrolase 20 (LOC5572305), transcript variant X3, mRNA.

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CloneID	OAe129334
Clone ID Related Accession (Same CDS sequence)	XM_021854464.1
Accession Version	XM_021854464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGAA GTGGGGGCAG CAAGTGTTCT CATCTGGACA ATGTTGTTCA TCTCTCGTTG 
GTCGAGCTGT GCAAGCTCAA GAAGGGTGAA CCATGTACGG AGTGCGACGC CAACGGTCCA 
AACCTGTGGA TATGTCTGCA GAAAAATTGT CTCCACATTG GCTGCTCGGA GCAGTACAAC 
GACCACAGTA CGACCCACTT CCAAACGAAC CGCACCCATT GCATCCACAT GAACCTGTCC 
TCTCAAAGGA TATGGTGCTA TTTGTGCGAG TGCGAAGTGT TCCTGGTGCA GCAACAACAA 
CAGCAACAGC AGCAGCTGAG ACGAACCTCG GTGGTGAGCA ACGATTCGAG TGATGCCAGT 
CGATACTCGG CCAACAACAA TAACGAGAAG CTGCTGATGT ATGGGGATCG GTTTGCCAGT 
GGATTTGGAG GTGGCGAATC CTGTGATAGC AGCTGCGAAG AAGACGAGTT TGAGAAGCAT 
GGTAAATGGA ATGGGCTGGT CGGGTTACAG AATATAGCAA ATACTTGCTA TATGAACTCG 
GCCATCCAAG CACTGAGCAA CAGTCCTCCT CTAACGGGAT ACTTCCTGGA TTGTGGGTCG 
ATCATAGAAA CCGATTTGCT CGGAAATCAA ACCGTTTCAA AGCCAGGTTT GGCCAAGCAT 
TACCACAAGT TGATAAAGGA AATGTGGTGT AAGAACAGAA GGTACGTTGT CCCCAGTGGA 
ATCCTGTATG GAATTCGAAT TGTACATCCT ATGTTCCGAG GCTACCAACA GCATGACACC 
CAAGAATTTC TTCGCTGCTT CATGGATCAG CTACATGAAG AGTTGAAGGA AGTTATTCAA 
CCACCGCCAG AGCTTTTATC ACTGCATGGC CGGAACGATG AAGACAATGA AGATCATTCA 
CCTTGTCCTT CGCCATCGCC TTCGCAATCC GAGGCAGAGT ATGAAACATG TGACAGCGGT 
GTTTCGGAGC AATCGAGCCT CTCGGATGAT ATATCAGTTA ATAATAAACG AACTAGAATG 
ATTTCGAGAT CTCCTAGCCC CACCGCAAAC CAAAGAAGTG CAACCTCGAA CAGTCGGCAA 
CAATCGCCGA TAGGAAGCAA TCGGAGCAAC TCCAGCAGCA GTGGAAGCAT GTCTTCTATG 
CAGCAGCAGC AGCAAGCCGG CTCCAGTGAT CAGCAACAAC AGAAACCTGC AAAGGACAAA 
CTTCCGGAAC GGTCCATCAT CAGCGACATA TTTGATGGGA AGCTTCTCTC ATCTGTACAA 
TGCTTAACCT GCGATCGTAT TTCAACTCGT GAAGAAACAT TTCAGGATCT ATCGTTACCA 
ATCCCCGGCA AAGATCATCT GGCAGTGTTG CATCAGAACC ACCCGGGAAT GCCTTCTCCT 
TCAGCTCATC CTTCGTCTCC GGTGGCTGGA AGTAGCAGTG GAATCACATG CTCTGATGCG 
GTCTATCCGG TAACCGGCGA TAGCTGGATG TGGTGGTTTT GGAACTGGCC TTTTTGGAGT 
TGGGTTCGAT CATGGTTCTG GGGACCAGCC GTTACACTGC ATGACTGCAT GGCAGCCTTC 
TTCAGCGCGG ACGAGCTGAA AGGAGACAAC ATGTACAGCT GCGAAAAGTG TAACAAACTC 
CGGAATGGGG TGAAGTTTTC CCGTGTTCTT GCCTTGCCTG AAATGTTGTG CGTCCATTTG 
AAACGTTTCC GGCACGATCT CTCCTACAGC TCGAAAATAT CCAGCCCCGT TAATTTCCCC 
ATGAACGGAC TAGACATGAG GCCATATCTG CACAAGGACT GCAAATCGGA AGTGACCTCG 
TACGATCTCT GTGCGGTCAT TTGTCACCAC GGCACGGTGG GAGGCGGACA TTATACCAGC 
TTTGCGAAAC ACGAACCCAC CGGGAAATGG TTCGAGTTCG ATGACCAGCT GGTGACGCAA 
GTCAGCCCGG AGGATGTCCA GAACTGTGAA GCTTATGTGC TGTTCTATCG CAAGAACAAC 
AGCAAAATGA GTACGATTCG GGCACAGGCC AGTGATCTGA TCAGCGTCCA GGAGCACGCT 
TCTGACATAA GGTTCTACAT TTCCCGGAAT TGGTTGCACA GATTCAACAC GTTTGCTGAA 
CCCGGACCGA TAGACAACTG GACGTTGCTC TGTCCCCATG GCGCGTTTCC ACCACACAAA 
GCTCCGTACT TTTCGAAGCT GGTAGTGCCA ATCCCGCAGA TCTTGTGGGA CTTTCTGTAC 
AAAAAGTTCG GTGGAGGCCC GGTGTGCAAT CACATCTTCC CGTGTCATAT TTGCAAGAAG 
GCAGCCGAAT CGTTGTCCAG GAGGCAACGA GCCGAGCTGG AAGAATTCAC CGCCTGTAAT 
GACGAGTATC AGCAGTACAA TGATACTCCA ACCACGATCT ACGCGATCTC GATGGCATGG 
TTCCGACAGT GGCAACTGTT TGCTCGCGGT GTCACCACGG AAGAACCAGG ACCTATCGAT 
AACAAAACCA TTGCAGTTCC GGGGGATACA AATGTTCCGC TTCGAAGCGT GCGCCAAGGA 
TCGGACTACG CCCAGATCAA CTCGGCCTTG TGGCACTTTT TCCACGGCAT CTACGGTGGA 
GGGCCGGAAA TCGTACTCCG CGGTAATCCG GTTCCGCCGC CAGAACCGAA AATCCCGAAA 
GTGGCTAAGG AACGAGAAAT TGAACCCATG GACGTGAGTG AACCAACTCC ACCAGTGAAA 
ACCACTGCTC CGGCAAGCGT GACCCAGCAG CATCAGCATC AGAAGGAGCA TTCCGCTGAA 
GCGACCACAG TGGATCCATC TCCAAGTACC CAATCTACAA CGGCGAAACC CCAGAAAAGT 
GTCTCCTTTG AGGACAACGA AGGTTACCAC GAAAACGACG ACGATCATAA CGAATCGTCT 
CGATCAGTGC AGTTGAAGCG CAAGCAGCTG AAGCAGCAGT CGAAGGAATC GAAGAAATCC 
GAATCCGACA GCGCCGATGG ACTGGTGACC TATCTTCGGC GGAAGCAACT CAAGCACCAA 
CAGCACGTGG CCACTAGCCA AGGGGCGGGA GGGGACGGTA ATACGGAATC GCTGGAGATT 
GTCAACAGGA AGGATCGTCG GCACCGGTCC GGAATGAAGT CGAACGGGTT TTTCGGGCCG 
GAAGGTATGT GGCAAAATAA GTGTGTAGGA CCCCGATTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021710156.1
CDS	1002..4163
Translation	MARSGGSKCSHLDNVVHLSLVELCKLKKGEPCTECDANGPNLWICLQKNCLHIGCSEQYNDHSTTHFQTNRTHCIHMNLSSQRIWCYLCECEVFLVQQQQQQQQQLRRTSVVSNDSSDASRYSANNNNEKLLMYGDRFASGFGGGESCDSSCEEDEFEKHGKWNGLVGLQNIANTCYMNSAIQALSNSPPLTGYFLDCGSIIETDLLGNQTVSKPGLAKHYHKLIKEMWCKNRRYVVPSGILYGIRIVHPMFRGYQQHDTQEFLRCFMDQLHEELKEVIQPPPELLSLHGRNDEDNEDHSPCPSPSPSQSEAEYETCDSGVSEQSSLSDDISVNNKRTRMISRSPSPTANQRSATSNSRQQSPIGSNRSNSSSSGSMSSMQQQQQAGSSDQQQQKPAKDKLPERSIISDIFDGKLLSSVQCLTCDRISTREETFQDLSLPIPGKDHLAVLHQNHPGMPSPSAHPSSPVAGSSSGITCSDAVYPVTGDSWMWWFWNWPFWSWVRSWFWGPAVTLHDCMAAFFSADELKGDNMYSCEKCNKLRNGVKFSRVLALPEMLCVHLKRFRHDLSYSSKISSPVNFPMNGLDMRPYLHKDCKSEVTSYDLCAVICHHGTVGGGHYTSFAKHEPTGKWFEFDDQLVTQVSPEDVQNCEAYVLFYRKNNSKMSTIRAQASDLISVQEHASDIRFYISRNWLHRFNTFAEPGPIDNWTLLCPHGAFPPHKAPYFSKLVVPIPQILWDFLYKKFGGGPVCNHIFPCHICKKAAESLSRRQRAELEEFTACNDEYQQYNDTPTTIYAISMAWFRQWQLFARGVTTEEPGPIDNKTIAVPGDTNVPLRSVRQGSDYAQINSALWHFFHGIYGGGPEIVLRGNPVPPPEPKIPKVAKEREIEPMDVSEPTPPVKTTAPASVTQQHQHQKEHSAEATTVDPSPSTQSTTAKPQKSVSFEDNEGYHENDDDHNESSRSVQLKRKQLKQQSKESKKSESDSADGLVTYLRRKQLKHQQHVATSQGAGGDGNTESLEIVNRKDRRHRSGMKSNGFFGPEGMWQNKCVGPRF

Target ORF information:

RefSeq Version	XM_021854464.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti ubiquitin carboxyl-terminal hydrolase 20 (LOC5572305), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021854464.1

ORF Insert Sequence:

ATGGCTCGAA GTGGGGGCAG CAAGTGTTCT CATCTGGACA ATGTTGTTCA TCTCTCGTTG 
GTCGAGCTGT GCAAGCTCAA GAAGGGTGAA CCATGTACGG AGTGCGACGC CAACGGTCCA 
AACCTGTGGA TATGTCTGCA GAAAAATTGT CTCCACATTG GCTGCTCGGA GCAGTACAAC 
GACCACAGTA CGACCCACTT CCAAACGAAC CGCACCCATT GCATCCACAT GAACCTGTCC 
TCTCAAAGGA TATGGTGCTA TTTGTGCGAG TGCGAAGTGT TCCTGGTGCA GCAACAACAA 
CAGCAACAGC AGCAGCTGAG ACGAACCTCG GTGGTGAGCA ACGATTCGAG TGATGCCAGT 
CGATACTCGG CCAACAACAA TAACGAGAAG CTGCTGATGT ATGGGGATCG GTTTGCCAGT 
GGATTTGGAG GTGGCGAATC CTGTGATAGC AGCTGCGAAG AAGACGAGTT TGAGAAGCAT 
GGTAAATGGA ATGGGCTGGT CGGGTTACAG AATATAGCAA ATACTTGCTA TATGAACTCG 
GCCATCCAAG CACTGAGCAA CAGTCCTCCT CTAACGGGAT ACTTCCTGGA TTGTGGGTCG 
ATCATAGAAA CCGATTTGCT CGGAAATCAA ACCGTTTCAA AGCCAGGTTT GGCCAAGCAT 
TACCACAAGT TGATAAAGGA AATGTGGTGT AAGAACAGAA GGTACGTTGT CCCCAGTGGA 
ATCCTGTATG GAATTCGAAT TGTACATCCT ATGTTCCGAG GCTACCAACA GCATGACACC 
CAAGAATTTC TTCGCTGCTT CATGGATCAG CTACATGAAG AGTTGAAGGA AGTTATTCAA 
CCACCGCCAG AGCTTTTATC ACTGCATGGC CGGAACGATG AAGACAATGA AGATCATTCA 
CCTTGTCCTT CGCCATCGCC TTCGCAATCC GAGGCAGAGT ATGAAACATG TGACAGCGGT 
GTTTCGGAGC AATCGAGCCT CTCGGATGAT ATATCAGTTA ATAATAAACG AACTAGAATG 
ATTTCGAGAT CTCCTAGCCC CACCGCAAAC CAAAGAAGTG CAACCTCGAA CAGTCGGCAA 
CAATCGCCGA TAGGAAGCAA TCGGAGCAAC TCCAGCAGCA GTGGAAGCAT GTCTTCTATG 
CAGCAGCAGC AGCAAGCCGG CTCCAGTGAT CAGCAACAAC AGAAACCTGC AAAGGACAAA 
CTTCCGGAAC GGTCCATCAT CAGCGACATA TTTGATGGGA AGCTTCTCTC ATCTGTACAA 
TGCTTAACCT GCGATCGTAT TTCAACTCGT GAAGAAACAT TTCAGGATCT ATCGTTACCA 
ATCCCCGGCA AAGATCATCT GGCAGTGTTG CATCAGAACC ACCCGGGAAT GCCTTCTCCT 
TCAGCTCATC CTTCGTCTCC GGTGGCTGGA AGTAGCAGTG GAATCACATG CTCTGATGCG 
GTCTATCCGG TAACCGGCGA TAGCTGGATG TGGTGGTTTT GGAACTGGCC TTTTTGGAGT 
TGGGTTCGAT CATGGTTCTG GGGACCAGCC GTTACACTGC ATGACTGCAT GGCAGCCTTC 
TTCAGCGCGG ACGAGCTGAA AGGAGACAAC ATGTACAGCT GCGAAAAGTG TAACAAACTC 
CGGAATGGGG TGAAGTTTTC CCGTGTTCTT GCCTTGCCTG AAATGTTGTG CGTCCATTTG 
AAACGTTTCC GGCACGATCT CTCCTACAGC TCGAAAATAT CCAGCCCCGT TAATTTCCCC 
ATGAACGGAC TAGACATGAG GCCATATCTG CACAAGGACT GCAAATCGGA AGTGACCTCG 
TACGATCTCT GTGCGGTCAT TTGTCACCAC GGCACGGTGG GAGGCGGACA TTATACCAGC 
TTTGCGAAAC ACGAACCCAC CGGGAAATGG TTCGAGTTCG ATGACCAGCT GGTGACGCAA 
GTCAGCCCGG AGGATGTCCA GAACTGTGAA GCTTATGTGC TGTTCTATCG CAAGAACAAC 
AGCAAAATGA GTACGATTCG GGCACAGGCC AGTGATCTGA TCAGCGTCCA GGAGCACGCT 
TCTGACATAA GGTTCTACAT TTCCCGGAAT TGGTTGCACA GATTCAACAC GTTTGCTGAA 
CCCGGACCGA TAGACAACTG GACGTTGCTC TGTCCCCATG GCGCGTTTCC ACCACACAAA 
GCTCCGTACT TTTCGAAGCT GGTAGTGCCA ATCCCGCAGA TCTTGTGGGA CTTTCTGTAC 
AAAAAGTTCG GTGGAGGCCC GGTGTGCAAT CACATCTTCC CGTGTCATAT TTGCAAGAAG 
GCAGCCGAAT CGTTGTCCAG GAGGCAACGA GCCGAGCTGG AAGAATTCAC CGCCTGTAAT 
GACGAGTATC AGCAGTACAA TGATACTCCA ACCACGATCT ACGCGATCTC GATGGCATGG 
TTCCGACAGT GGCAACTGTT TGCTCGCGGT GTCACCACGG AAGAACCAGG ACCTATCGAT 
AACAAAACCA TTGCAGTTCC GGGGGATACA AATGTTCCGC TTCGAAGCGT GCGCCAAGGA 
TCGGACTACG CCCAGATCAA CTCGGCCTTG TGGCACTTTT TCCACGGCAT CTACGGTGGA 
GGGCCGGAAA TCGTACTCCG CGGTAATCCG GTTCCGCCGC CAGAACCGAA AATCCCGAAA 
GTGGCTAAGG AACGAGAAAT TGAACCCATG GACGTGAGTG AACCAACTCC ACCAGTGAAA 
ACCACTGCTC CGGCAAGCGT GACCCAGCAG CATCAGCATC AGAAGGAGCA TTCCGCTGAA 
GCGACCACAG TGGATCCATC TCCAAGTACC CAATCTACAA CGGCGAAACC CCAGAAAAGT 
GTCTCCTTTG AGGACAACGA AGGTTACCAC GAAAACGACG ACGATCATAA CGAATCGTCT 
CGATCAGTGC AGTTGAAGCG CAAGCAGCTG AAGCAGCAGT CGAAGGAATC GAAGAAATCC 
GAATCCGACA GCGCCGATGG ACTGGTGACC TATCTTCGGC GGAAGCAACT CAAGCACCAA 
CAGCACGTGG CCACTAGCCA AGGGGCGGGA GGGGACGGTA ATACGGAATC GCTGGAGATT 
GTCAACAGGA AGGATCGTCG GCACCGGTCC GGAATGAAGT CGAACGGGTT TTTCGGGCCG 
GAAGGTATGT GGCAAAATAA GTGTGTAGGA CCCCGATTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5572305 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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