AAEL_AAEL009258 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL009258
Entrez Gene ID	5571743
Full Name	uncharacterized LOC5571743
Synonyms	AAEL009258
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021839308.1	XP_021695000.1	uncharacterized protein LOC5571743
XM_001659838.1	XP_001659888.1	AAEL009258-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL009258 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL009258 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe07139	XM_001659838.1 Latest version!	Aedes aegypti AAEL009258-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe117981	XM_021839308.1 Latest version!	Aedes aegypti uncharacterized LOC5571743 (LOC5571743), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL009258 ( XM_001659838.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001659888.1 Aedes aegypti AAEL009258-RA partial mRNA.

Price & Availability↑

CloneID	OAe07139
Clone ID Related Accession (Same CDS sequence)	XM_001659838.1
Accession Version	XM_001659838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL009258-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811113). COMPLETENESS: incomplete on both ends.

ATGGTCCAAT TCGACTGGCG GCGTATGTGT CGCAAGAAGC GAAAGGTTCC TCAGGGCGGT 
GCTATCCTGG ATCAAGTGAT TTCCCAGTCG GCCAATGCTT CTAATCAGTG CTTGCTGTAT 
AAGATGGCTA ATTACAAACG GGGTGGAGAT TTGATCGATG CGTTCCAGGT CGGAGGGCAG 
AAGGCCGTGG AGCAGTTGAT TCGGGAGCAG TTTGGAGTTT TTATGTACAA CAATGGCCGC 
GGACAGATCA TCAATAGGGC CGAGTATCTA CGGTGGAAGT ACATGGATAA TCATGAGGTC 
GTCATCCCGA TTGAGGCGTC GTTGTCTCCG CATGATCCTT TGGGCAAATG GGTCGATCAT 
AAGGCCTGCT GGCAGATGCA GTATCGTGGA TTGCTGGGCG AGAGTTTGCT GCACGTGTTG 
ATCATCTGCG ATACGAAGAT TCACACGAAG TTGGCCCGCA TCCTGCTGCG CGTGTTCCCC 
GAGCTGTCGA TCGACGTGAT GGAAGGCGAG GAGTACCTGG GCGCTAGTGC ACTCCACTTG 
GCGATCGCGT ACAGCAACAA CGAGTTGGTG GGCGATCTGA TCGATGCCGG AGCGGACGTG 
TCCCAGCGCG CCATCGGACG GTTCTTCCTT CCACGGGATC AGCAGGGCTT GAAGCCGGCC 
AAGAACACCG ACTACGAGGG GTTGGCCTAC TTGGGCGAGT ATCCGCTGGC GTGGGCCGCC 
TGCTGCGCCA ATGAGTCCGT CTATAACCTG CTGCTGGAAT GCGGGGCGGA TCCGAACGCG 
CAGGACAGCT TCGGGAACAT GATTCTGCAC ATGGTCGTCG TCTGCGATAA GCTGGATATG 
TTCGGGTATG CCCTTCGTCA TCCGAAGCTT CCCTGTAAAA ATGGGATCGT CAATGAGTCC 
GGACTCACGC CGTTGACCTT GGCCTGTCGA TTGGGTCGTG ATGAAGTGTT CCGTGAGATG 
TTGGAGTTGT CGGCGAGGGA GTTTTGGCGG TACAGTAACA TCACCTGTTC CGGATATCCG 
TTGAACGCAT TGGACACGTT GATGCCCGAC GGAACTACCA ATTGGAATTC GGCGCTGTTT 
ATCATCCTCA ACGGAACCAA GGAAGAGCAT TTGAACATGC TGGACGGTGG CATCGTGGAA 
CGTCTGCTGG ACGAGAAGTG GAAGACGTTC GCTCGGAACC AGTTCATCAA GCGACTGCTG 
ATCCTCGCTC TGCATCTGTT CTGCTTGTCG TGTTCGGTCT ACTTGAGACC GGTGCGAGTT 
TTCAAGGAGG AGAGTGAAGA GGGAAGCGAT GGAGGAGATG GAGCGGGTAC AGCTGCTCCG 
GACGGTGTCG ATGATGATGA GGACATTGAT TTGACGACCT GGTTCCGGTA TGGATTCGAG 
ATCGCAACCG TGATGGGAGT ACTGAGCTAC GTGGTTCTTC AACAGGGTGA TGAGATCAAG 
AATCAGGGAT TGATCTCGTT TATAAAGTCA CTTGGAAATG CTCCGGCGAA AGCTATCTTC 
CTGATCTCCA ATCTGATGAT CTTGGCTTGC ATCCCGTTCC GAATGATGGG TGACGTTGAA 
CACGAGGAGG CCATTCTGTT GTTCGCAGTC CCAGGATCAT GGTTCCTGCT GATGTTCTTT 
GCTGGCGCCA TCGGCTTAAC AGGTCCCTTC GTCACCATGA TCTTCTCTAT GATCACGGGT 
GACATGTTCA CCTTCGGCAT CATCTACATG ATCGTTCTGT TCGGATTTTC CCAGGCGTTC 
TACTTCCTGT ACAAAGGTCA TCCGGAAGCG GAGGATTCTC CATTTGGGAG CTACTTCGGC 
ACTTGGATGG GCCTGTTCCA GACAACCCTC GGCGATTACG ATTACGCCGA CTTGAATCTT 
ACGACCTACC CGAACCTGGC GAAAACCGTG TTTATCATAT TCATGATCTT CGTGCCGATC 
CTGTTGTTGA ACATGTTGAT CGCTATGATG GGCAACACCT ACGCGTACGT CATTGAGCAA 
GCTGAAAAGG AAGGCATGAA ACAGTGGGCC AAGATCGTGG TCAACCTGGA GCGGGCGGTC 
AAGCAGGAGG ACGCCAAAAA GTACCTGGAG GAGTACTCGA TCGGGTTGGG ACCGTCGGAT 
GATCCGAGGT ACGAGATCCG TGGAGTCATG GTCATCAAGA GCAAGAGTAA GACCCGTGCC 
AGGCAGCGCA AGGGAGCGGT AAGTAACTGG AAGAGCGTTC TGCGTGTCAC GTTGAACGAG 
CTGAAGAAGC GTAACATGAC CGGCGAGGAG CTGCGGAGGA TCATGTGGGG ACGATCGTCG 
ATCACTTCTC CGGCCAAGAT CTCCAAGAAG AAAAAGATCT ACGAAGAGGA GGACCCCTTC 
GCCATCACCA ATGCTATCGA TGTGATGTCC TTCACGCAGG ACATCGTCAT GGTAAGTAGC 
GAACCGACCG GACCGCCCAC AATCGACCCC TCCAAACCGG CGACGATCGC CGTCAAGCCC 
CAAACGGCAC CCGCAGCGCA ACCGATCCCG GAGATTCCTC CTCAACCGCC GCTGGTCAAA 
CGGCAGTCCG TGGTTAGTGC TCCGCCGGCC TACGACGATT TCCCCCCGAT GAAGGACTAC 
AAGGATCCCC TGCGGGAGCT GGTCATCATC TCGGAGTCGC CCTCGGTGGA CGACCATTAC 
GTGCAGAGCT GCAAGACCCT CGCGAATGAT GCGTCCACCC TGGATCACGA CACCTTGGGT 
CAGTTAAATC CCTTCCTGGA GGCGAAGGAC GTGGTCGATC CGGTGAAGGA ACGCGAGTTT 
CTCAAAACAC TGGAGGCCCT GGAGGACACC GACAGCGAAG CGGCCGAAAA GCCGGTCCTG 
GGCAAGATCT CCCTGATACG ACGCGCCAAG TCAGCCGTCT CGAGAACCAC TTCCAGGAAG 
AAGAAAACCG ACCAGCATCC GCTGTTTACG ATCGCTTGGG ATGACAAGAA CCTGACCCGG 
ACGCATCCGG AGGACTTTGG AGCGCTGAAC ACGGCCTACG AGTACAGTGC CGAAGATTTC 
AAGCAAGACG TGGACGAGGA AGCGAGGGAG GAGGAGGAAG ACGAGGGTGT GACGGTGGAG 
GAAGTGCACC GCCGAATGGA AGAGCTACAC CATCGCGGCC GGGCTTCCTT GGAGCGGGAC 
AGCAACTCGA CCGAGAGCAG CAAGAAACAT CCGCACAGGA AGCGGCAAGG TATGGGCCGA 
GGTCGGAACA ACAAGGTGTC GCCGGATACG TCCAACGAGA GCGTATCCGG CAAGAAGGAC 
AAACGGATGA AGTCGGCGCC GACGGGTGGA GGGACCTCGC CACCGGATCC ACTGGAACCA 
TGGAGCACAA GGGATATTTG CAACATTAAT AAACTTTTGG ACACCGATAC GCAGGATGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001659888.1
CDS	1..3363
Translation	MVQFDWRRMCRKKRKVPQGGAILDQVISQSANASNQCLLYKMANYKRGGDLIDAFQVGGQKAVEQLIREQFGVFMYNNGRGQIINRAEYLRWKYMDNHEVVIPIEASLSPHDPLGKWVDHKACWQMQYRGLLGESLLHVLIICDTKIHTKLARILLRVFPELSIDVMEGEEYLGASALHLAIAYSNNELVGDLIDAGADVSQRAIGRFFLPRDQQGLKPAKNTDYEGLAYLGEYPLAWAACCANESVYNLLLECGADPNAQDSFGNMILHMVVVCDKLDMFGYALRHPKLPCKNGIVNESGLTPLTLACRLGRDEVFREMLELSAREFWRYSNITCSGYPLNALDTLMPDGTTNWNSALFIILNGTKEEHLNMLDGGIVERLLDEKWKTFARNQFIKRLLILALHLFCLSCSVYLRPVRVFKEESEEGSDGGDGAGTAAPDGVDDDEDIDLTTWFRYGFEIATVMGVLSYVVLQQGDEIKNQGLISFIKSLGNAPAKAIFLISNLMILACIPFRMMGDVEHEEAILLFAVPGSWFLLMFFAGAIGLTGPFVTMIFSMITGDMFTFGIIYMIVLFGFSQAFYFLYKGHPEAEDSPFGSYFGTWMGLFQTTLGDYDYADLNLTTYPNLAKTVFIIFMIFVPILLLNMLIAMMGNTYAYVIEQAEKEGMKQWAKIVVNLERAVKQEDAKKYLEEYSIGLGPSDDPRYEIRGVMVIKSKSKTRARQRKGAVSNWKSVLRVTLNELKKRNMTGEELRRIMWGRSSITSPAKISKKKKIYEEEDPFAITNAIDVMSFTQDIVMVSSEPTGPPTIDPSKPATIAVKPQTAPAAQPIPEIPPQPPLVKRQSVVSAPPAYDDFPPMKDYKDPLRELVIISESPSVDDHYVQSCKTLANDASTLDHDTLGQLNPFLEAKDVVDPVKEREFLKTLEALEDTDSEAAEKPVLGKISLIRRAKSAVSRTTSRKKKTDQHPLFTIAWDDKNLTRTHPEDFGALNTAYEYSAEDFKQDVDEEAREEEEDEGVTVEEVHRRMEELHHRGRASLERDSNSTESSKKHPHRKRQGMGRGRNNKVSPDTSNESVSGKKDKRMKSAPTGGGTSPPDPLEPWSTRDICNINKLLDTDTQDE

Target ORF information:

RefSeq Version	XM_001659838.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL009258-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001659838.1

ORF Insert Sequence:

ATGGTCCAAT TCGACTGGCG GCGTATGTGT CGCAAGAAGC GAAAGGTTCC TCAGGGCGGT 
GCTATCCTGG ATCAAGTGAT TTCCCAGTCG GCCAATGCTT CTAATCAGTG CTTGCTGTAT 
AAGATGGCTA ATTACAAACG GGGTGGAGAT TTGATCGATG CGTTCCAGGT CGGAGGGCAG 
AAGGCCGTGG AGCAGTTGAT TCGGGAGCAG TTTGGAGTTT TTATGTACAA CAATGGCCGC 
GGACAGATCA TCAATAGGGC CGAGTATCTA CGGTGGAAGT ACATGGATAA TCATGAGGTC 
GTCATCCCGA TTGAGGCGTC GTTGTCTCCG CATGATCCTT TGGGCAAATG GGTCGATCAT 
AAGGCCTGCT GGCAGATGCA GTATCGTGGA TTGCTGGGCG AGAGTTTGCT GCACGTGTTG 
ATCATCTGCG ATACGAAGAT TCACACGAAG TTGGCCCGCA TCCTGCTGCG CGTGTTCCCC 
GAGCTGTCGA TCGACGTGAT GGAAGGCGAG GAGTACCTGG GCGCTAGTGC ACTCCACTTG 
GCGATCGCGT ACAGCAACAA CGAGTTGGTG GGCGATCTGA TCGATGCCGG AGCGGACGTG 
TCCCAGCGCG CCATCGGACG GTTCTTCCTT CCACGGGATC AGCAGGGCTT GAAGCCGGCC 
AAGAACACCG ACTACGAGGG GTTGGCCTAC TTGGGCGAGT ATCCGCTGGC GTGGGCCGCC 
TGCTGCGCCA ATGAGTCCGT CTATAACCTG CTGCTGGAAT GCGGGGCGGA TCCGAACGCG 
CAGGACAGCT TCGGGAACAT GATTCTGCAC ATGGTCGTCG TCTGCGATAA GCTGGATATG 
TTCGGGTATG CCCTTCGTCA TCCGAAGCTT CCCTGTAAAA ATGGGATCGT CAATGAGTCC 
GGACTCACGC CGTTGACCTT GGCCTGTCGA TTGGGTCGTG ATGAAGTGTT CCGTGAGATG 
TTGGAGTTGT CGGCGAGGGA GTTTTGGCGG TACAGTAACA TCACCTGTTC CGGATATCCG 
TTGAACGCAT TGGACACGTT GATGCCCGAC GGAACTACCA ATTGGAATTC GGCGCTGTTT 
ATCATCCTCA ACGGAACCAA GGAAGAGCAT TTGAACATGC TGGACGGTGG CATCGTGGAA 
CGTCTGCTGG ACGAGAAGTG GAAGACGTTC GCTCGGAACC AGTTCATCAA GCGACTGCTG 
ATCCTCGCTC TGCATCTGTT CTGCTTGTCG TGTTCGGTCT ACTTGAGACC GGTGCGAGTT 
TTCAAGGAGG AGAGTGAAGA GGGAAGCGAT GGAGGAGATG GAGCGGGTAC AGCTGCTCCG 
GACGGTGTCG ATGATGATGA GGACATTGAT TTGACGACCT GGTTCCGGTA TGGATTCGAG 
ATCGCAACCG TGATGGGAGT ACTGAGCTAC GTGGTTCTTC AACAGGGTGA TGAGATCAAG 
AATCAGGGAT TGATCTCGTT TATAAAGTCA CTTGGAAATG CTCCGGCGAA AGCTATCTTC 
CTGATCTCCA ATCTGATGAT CTTGGCTTGC ATCCCGTTCC GAATGATGGG TGACGTTGAA 
CACGAGGAGG CCATTCTGTT GTTCGCAGTC CCAGGATCAT GGTTCCTGCT GATGTTCTTT 
GCTGGCGCCA TCGGCTTAAC AGGTCCCTTC GTCACCATGA TCTTCTCTAT GATCACGGGT 
GACATGTTCA CCTTCGGCAT CATCTACATG ATCGTTCTGT TCGGATTTTC CCAGGCGTTC 
TACTTCCTGT ACAAAGGTCA TCCGGAAGCG GAGGATTCTC CATTTGGGAG CTACTTCGGC 
ACTTGGATGG GCCTGTTCCA GACAACCCTC GGCGATTACG ATTACGCCGA CTTGAATCTT 
ACGACCTACC CGAACCTGGC GAAAACCGTG TTTATCATAT TCATGATCTT CGTGCCGATC 
CTGTTGTTGA ACATGTTGAT CGCTATGATG GGCAACACCT ACGCGTACGT CATTGAGCAA 
GCTGAAAAGG AAGGCATGAA ACAGTGGGCC AAGATCGTGG TCAACCTGGA GCGGGCGGTC 
AAGCAGGAGG ACGCCAAAAA GTACCTGGAG GAGTACTCGA TCGGGTTGGG ACCGTCGGAT 
GATCCGAGGT ACGAGATCCG TGGAGTCATG GTCATCAAGA GCAAGAGTAA GACCCGTGCC 
AGGCAGCGCA AGGGAGCGGT AAGTAACTGG AAGAGCGTTC TGCGTGTCAC GTTGAACGAG 
CTGAAGAAGC GTAACATGAC CGGCGAGGAG CTGCGGAGGA TCATGTGGGG ACGATCGTCG 
ATCACTTCTC CGGCCAAGAT CTCCAAGAAG AAAAAGATCT ACGAAGAGGA GGACCCCTTC 
GCCATCACCA ATGCTATCGA TGTGATGTCC TTCACGCAGG ACATCGTCAT GGTAAGTAGC 
GAACCGACCG GACCGCCCAC AATCGACCCC TCCAAACCGG CGACGATCGC CGTCAAGCCC 
CAAACGGCAC CCGCAGCGCA ACCGATCCCG GAGATTCCTC CTCAACCGCC GCTGGTCAAA 
CGGCAGTCCG TGGTTAGTGC TCCGCCGGCC TACGACGATT TCCCCCCGAT GAAGGACTAC 
AAGGATCCCC TGCGGGAGCT GGTCATCATC TCGGAGTCGC CCTCGGTGGA CGACCATTAC 
GTGCAGAGCT GCAAGACCCT CGCGAATGAT GCGTCCACCC TGGATCACGA CACCTTGGGT 
CAGTTAAATC CCTTCCTGGA GGCGAAGGAC GTGGTCGATC CGGTGAAGGA ACGCGAGTTT 
CTCAAAACAC TGGAGGCCCT GGAGGACACC GACAGCGAAG CGGCCGAAAA GCCGGTCCTG 
GGCAAGATCT CCCTGATACG ACGCGCCAAG TCAGCCGTCT CGAGAACCAC TTCCAGGAAG 
AAGAAAACCG ACCAGCATCC GCTGTTTACG ATCGCTTGGG ATGACAAGAA CCTGACCCGG 
ACGCATCCGG AGGACTTTGG AGCGCTGAAC ACGGCCTACG AGTACAGTGC CGAAGATTTC 
AAGCAAGACG TGGACGAGGA AGCGAGGGAG GAGGAGGAAG ACGAGGGTGT GACGGTGGAG 
GAAGTGCACC GCCGAATGGA AGAGCTACAC CATCGCGGCC GGGCTTCCTT GGAGCGGGAC 
AGCAACTCGA CCGAGAGCAG CAAGAAACAT CCGCACAGGA AGCGGCAAGG TATGGGCCGA 
GGTCGGAACA ACAAGGTGTC GCCGGATACG TCCAACGAGA GCGTATCCGG CAAGAAGGAC 
AAACGGATGA AGTCGGCGCC GACGGGTGGA GGGACCTCGC CACCGGATCC ACTGGAACCA 
TGGAGCACAA GGGATATTTG CAACATTAAT AAACTTTTGG ACACCGATAC GCAGGATGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL009258 ( XM_021839308.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021695000.1 Aedes aegypti uncharacterized LOC5571743 (LOC5571743), mRNA.

Price & Availability↑

CloneID	OAe117981
Clone ID Related Accession (Same CDS sequence)	XM_021839308.1
Accession Version	XM_021839308.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5571743
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAAT TCGACTGGCG GCGTATGTGT CGCAAGAAGC GAAAGGTTCC TCAGGGCGGT 
GCTATCCTGG ATCAAGTGAT TTCCCAGTCG GCCAATGCTT CTAATCAGTG CTTGCTGTAT 
AAGATGGCTA ATTACAAACG GGGTGGAGAT TTGATCGATG CGTTCCAGGT CGGAGGGCAG 
AAGGCCGTGG AGCAGTTGAT TCGGGAGCAG TTTGGAGTTT TTATGTACAA CAATGGCCGC 
GGACAGATCA TCAATAGGGC CGAGTATCTA CGGTGGAAGT ACATGGATAA TCATGAGGTC 
GTCATCCCGA TTGAGGCGTC GTTGTCTCCG CATGATCCTT TGGGCAAATG GGTCGATCAT 
AAGGCCTGCT GGCAGATGCA GTATCGTGGA TTGCTGGGCG AGAGTTTGCT GCACGTGTTG 
ATCATCTGCG ATACGAAGAT TCACACGAAG TTGGCCCGCA TCCTGCTGCG CGTGTTCCCC 
GAGCTGTCGA TCGACGTGAT GGAAGGCGAG GAGTACCTGG GCGCTAGTGC ACTCCACTTG 
GCGATCGCGT ACAGCAACAA CGAGTTGGTG GGCGATCTGA TCGATGCCGG AGCGGACGTG 
TCCCAGCGCG CCATCGGACG GTTCTTCCTT CCACGGGATC AGCAGGGCTT GAAGCCGGCC 
AAGAACACCG ACTACGAGGG GTTGGCCTAC TTGGGCGAGT ATCCGCTGGC GTGGGCCGCC 
TGCTGCGCCA ATGAGTCCGT CTATAACCTG CTGCTGGAAT GCGGGGCGGA TCCGAACGCG 
CAGGACAGCT TCGGGAACAT GATTCTGCAC ATGGTCGTCG TCTGCGATAA GCTGGATATG 
TTCGGGTATG CCCTTCGTCA TCCGAAGCTT CCCTGTAAAA ATGGGATCGT CAATGAGTCC 
GGACTCACGC CGTTGACCTT GGCCTGTCGA TTGGGTCGTG ATGAAGTGTT CCGTGAGATG 
TTGGAGTTGT CGGCGAGGGA GTTTTGGCGG TACAGTAACA TCACCTGTTC CGGATATCCG 
TTGAACGCAT TGGACACGTT GATGCCCGAC GGAACTACCA ATTGGAATTC GGCGCTGTTT 
ATCATCCTCA ACGGAACCAA GGAAGAGCAT TTGAACATGC TGGACGGTGG CATCGTGGAA 
CGTCTGCTGG ACGAGAAGTG GAAGACGTTC GCTCGGAACC AGTTCATCAA GCGACTGCTG 
ATCCTCGCTC TGCATCTGTT CTGCTTGTCG TGCTCGGTCT ACTTGAGACC GGTGCGAGTT 
TTCAAGGAGG AGAGTGAAGA GGGAAGCGAT GGAGGAGATG GAGTGGGTAC AGCTGCTCCG 
GACGGTGTCG ATGATGATGA GGACATTGAT TTGACGACCT GGTTCCGGTA TGGATTCGAG 
ATCGCAACCG TGATGGGAGT ACTGAGCTAC GTGGTTCTTC AACAGGGTGA TGAGATCAAG 
AATCAGGGAT TGATCTCGTT TATAAAGTCA CTTGGAAATG CTCCGGCGAA AGCTATCTTC 
CTGATCTCCA ATCTGATGAT CTTGGCTTGC ATCCCGTTCC GAATGATGGG TGACGTTGAA 
CACGAGGAGG CCATTCTGTT GTTCGCAGTC CCAGGATCAT GGTTCCTGCT GATGTTCTTT 
GCTGGCGCCA TCGGCTTAAC AGGTCCCTTC GTCACCATGA TCTTCTCTAT GATCACGGGT 
GACATGTTCA CCTTCGGCAT CATCTACATG ATCGTTCTGT TCGGATTTTC CCAGGCGTTC 
TACTTCCTGT ACAAAGGTCA TCCGGAAGCG GAGGATTCTC CATTTGGGAG CTACTTCGGC 
ACTTGGATGG GCCTGTTCCA GACAACCCTC GGCGATTACG ATTACGCCGA CTTGAATCTT 
ACGACCTACC CGAACCTGGC GAAAACCGTG TTTATCATAT TCATGATCTT CGTGCCGATC 
CTGTTGTTGA ACATGTTGAT CGCTATGATG GGCAACACCT ACGCGTACGT CATTGAGCAA 
GCTGAAAAGG AAGGCATGAA ACAGTGGGCC AAGATCGTGG TCAACCTGGA GCGGGCGGTC 
AAGCAGGAGG ACGCCAAAAA GTACCTGGAG GAGTACTCGA TCGGGTTGGG ACCGTCGGAT 
GATCCGAGGT ACGAGATCCG TGGAGTCATG GTCATCAAGA GCAAGAGTAA GACCCGTGCC 
AGGCAGCGCA AGGGAGCGGT AAGTAACTGG AAGAGCGTTC TGCGTGTCAC GTTGAACGAG 
CTGAAGAAGC GTAACATGAC CGGCGAGGAG CTGCGGAGGA TCATGTGGGG ACGATCGTCG 
ATCACTTCTC CGGCCAAGAT CTCCAAGAAG AAAAAGATCT ACGAAGAGGA GGACCCCTTC 
GCCATCACCA ATGCTATCGA TGTGATGTCC TTCACGCAGG ACATCGTCAT GGTAAGTAGC 
GAACCGACCG GACCGCCCAC AATCGACCCC TCCAAACCGG CGACGATCGC CGTCAAGCCC 
CAAACGGCAC CCGCAGCGCA ACCGATCCCG GAGATTCCTC CTCAACCGCC GCTGGTCAAA 
CGGCAGTCCG TGGTTAGTGC TCCGCCGGCC TACGACGATT TCCCCCCGAT GAAGGACTAC 
AAGGATCCCC TGCGGGAGCT GGTCATCATC TCGGAGTCGC CCTCGGTGGA CGACCATTAC 
GTGCAGAGCT GCAAGACCCT CGCGAATGAT GCGTCCACCC TGGATCACGT GCACGAGATC 
AACCTGACCA AGCAGAACCA GCTCCGGCAG GAGGAACAGA AGCAACAACA ACAGCAGACC 
TTGGGTCAGT TAAATCCCTT CCTGGAGGCG AAGGACGTGG TCGATCCGGT GAAGGAACGC 
GAGTTTCTCA AAACACTGGA GGCCCTGGAG GACACCGACA GCGAAGCGGC CGAAAAGCCG 
GTCCTGGGCA AGATCTCCCT GATACGACGC GCCAAGTCAG CCGTCTCGAG AACCACTTCC 
AGGAAGAAGA AAACCGACCA GCATCCGCTG TTCACGATCG CTTGGGATGA CAAGAACCTG 
ACCCGGACCC ATCCGGAGGA CTTTGGAGCG CTGAACACGG CCTACGAGTA CAGTGCCGAA 
GATTTCAAGC AAGACGTGGA CGAGGAAGCG AGGGAGGAGG AGGAAGACGA GGGTGTGACG 
GTGGAGGAAG TGCACCGCCG AATGGAAGAG CTGCACCATC GCGGCCGGGC TTCCTTGGAG 
CGGGACAGCA ACTCGACCGA GAGCAGCAAG AAACATCCGC ACAGGAAGCG GCAAGGTATG 
GGCCGAGGTC GGAACAACAA GGTGTCGCCG GATACGTCCA ACGAGAGCGT ATCCGGCAAG 
AAGGACAAAC GGATGAAGTC GGCGCCGACG GGTGGAGGTG GAGTTCGTCA TCGGCAGCTT 
ATGGACCAGG AAACGGGAGG GACCTCGCCA CCGGATCCAC TGGAACCATG GAGCACAAGG 
GATATTTGCA ACATTAATAA ACTTTTGGAC ACCGATACGC AGGATGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021695000.1
CDS	130..3600
Translation	MVQFDWRRMCRKKRKVPQGGAILDQVISQSANASNQCLLYKMANYKRGGDLIDAFQVGGQKAVEQLIREQFGVFMYNNGRGQIINRAEYLRWKYMDNHEVVIPIEASLSPHDPLGKWVDHKACWQMQYRGLLGESLLHVLIICDTKIHTKLARILLRVFPELSIDVMEGEEYLGASALHLAIAYSNNELVGDLIDAGADVSQRAIGRFFLPRDQQGLKPAKNTDYEGLAYLGEYPLAWAACCANESVYNLLLECGADPNAQDSFGNMILHMVVVCDKLDMFGYALRHPKLPCKNGIVNESGLTPLTLACRLGRDEVFREMLELSAREFWRYSNITCSGYPLNALDTLMPDGTTNWNSALFIILNGTKEEHLNMLDGGIVERLLDEKWKTFARNQFIKRLLILALHLFCLSCSVYLRPVRVFKEESEEGSDGGDGVGTAAPDGVDDDEDIDLTTWFRYGFEIATVMGVLSYVVLQQGDEIKNQGLISFIKSLGNAPAKAIFLISNLMILACIPFRMMGDVEHEEAILLFAVPGSWFLLMFFAGAIGLTGPFVTMIFSMITGDMFTFGIIYMIVLFGFSQAFYFLYKGHPEAEDSPFGSYFGTWMGLFQTTLGDYDYADLNLTTYPNLAKTVFIIFMIFVPILLLNMLIAMMGNTYAYVIEQAEKEGMKQWAKIVVNLERAVKQEDAKKYLEEYSIGLGPSDDPRYEIRGVMVIKSKSKTRARQRKGAVSNWKSVLRVTLNELKKRNMTGEELRRIMWGRSSITSPAKISKKKKIYEEEDPFAITNAIDVMSFTQDIVMVSSEPTGPPTIDPSKPATIAVKPQTAPAAQPIPEIPPQPPLVKRQSVVSAPPAYDDFPPMKDYKDPLRELVIISESPSVDDHYVQSCKTLANDASTLDHVHEINLTKQNQLRQEEQKQQQQQTLGQLNPFLEAKDVVDPVKEREFLKTLEALEDTDSEAAEKPVLGKISLIRRAKSAVSRTTSRKKKTDQHPLFTIAWDDKNLTRTHPEDFGALNTAYEYSAEDFKQDVDEEAREEEEDEGVTVEEVHRRMEELHHRGRASLERDSNSTESSKKHPHRKRQGMGRGRNNKVSPDTSNESVSGKKDKRMKSAPTGGGGVRHRQLMDQETGGTSPPDPLEPWSTRDICNINKLLDTDTQDE

Target ORF information:

RefSeq Version	XM_021839308.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti uncharacterized LOC5571743 (LOC5571743), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021839308.1

ORF Insert Sequence:

ATGGTCCAAT TCGACTGGCG GCGTATGTGT CGCAAGAAGC GAAAGGTTCC TCAGGGCGGT 
GCTATCCTGG ATCAAGTGAT TTCCCAGTCG GCCAATGCTT CTAATCAGTG CTTGCTGTAT 
AAGATGGCTA ATTACAAACG GGGTGGAGAT TTGATCGATG CGTTCCAGGT CGGAGGGCAG 
AAGGCCGTGG AGCAGTTGAT TCGGGAGCAG TTTGGAGTTT TTATGTACAA CAATGGCCGC 
GGACAGATCA TCAATAGGGC CGAGTATCTA CGGTGGAAGT ACATGGATAA TCATGAGGTC 
GTCATCCCGA TTGAGGCGTC GTTGTCTCCG CATGATCCTT TGGGCAAATG GGTCGATCAT 
AAGGCCTGCT GGCAGATGCA GTATCGTGGA TTGCTGGGCG AGAGTTTGCT GCACGTGTTG 
ATCATCTGCG ATACGAAGAT TCACACGAAG TTGGCCCGCA TCCTGCTGCG CGTGTTCCCC 
GAGCTGTCGA TCGACGTGAT GGAAGGCGAG GAGTACCTGG GCGCTAGTGC ACTCCACTTG 
GCGATCGCGT ACAGCAACAA CGAGTTGGTG GGCGATCTGA TCGATGCCGG AGCGGACGTG 
TCCCAGCGCG CCATCGGACG GTTCTTCCTT CCACGGGATC AGCAGGGCTT GAAGCCGGCC 
AAGAACACCG ACTACGAGGG GTTGGCCTAC TTGGGCGAGT ATCCGCTGGC GTGGGCCGCC 
TGCTGCGCCA ATGAGTCCGT CTATAACCTG CTGCTGGAAT GCGGGGCGGA TCCGAACGCG 
CAGGACAGCT TCGGGAACAT GATTCTGCAC ATGGTCGTCG TCTGCGATAA GCTGGATATG 
TTCGGGTATG CCCTTCGTCA TCCGAAGCTT CCCTGTAAAA ATGGGATCGT CAATGAGTCC 
GGACTCACGC CGTTGACCTT GGCCTGTCGA TTGGGTCGTG ATGAAGTGTT CCGTGAGATG 
TTGGAGTTGT CGGCGAGGGA GTTTTGGCGG TACAGTAACA TCACCTGTTC CGGATATCCG 
TTGAACGCAT TGGACACGTT GATGCCCGAC GGAACTACCA ATTGGAATTC GGCGCTGTTT 
ATCATCCTCA ACGGAACCAA GGAAGAGCAT TTGAACATGC TGGACGGTGG CATCGTGGAA 
CGTCTGCTGG ACGAGAAGTG GAAGACGTTC GCTCGGAACC AGTTCATCAA GCGACTGCTG 
ATCCTCGCTC TGCATCTGTT CTGCTTGTCG TGCTCGGTCT ACTTGAGACC GGTGCGAGTT 
TTCAAGGAGG AGAGTGAAGA GGGAAGCGAT GGAGGAGATG GAGTGGGTAC AGCTGCTCCG 
GACGGTGTCG ATGATGATGA GGACATTGAT TTGACGACCT GGTTCCGGTA TGGATTCGAG 
ATCGCAACCG TGATGGGAGT ACTGAGCTAC GTGGTTCTTC AACAGGGTGA TGAGATCAAG 
AATCAGGGAT TGATCTCGTT TATAAAGTCA CTTGGAAATG CTCCGGCGAA AGCTATCTTC 
CTGATCTCCA ATCTGATGAT CTTGGCTTGC ATCCCGTTCC GAATGATGGG TGACGTTGAA 
CACGAGGAGG CCATTCTGTT GTTCGCAGTC CCAGGATCAT GGTTCCTGCT GATGTTCTTT 
GCTGGCGCCA TCGGCTTAAC AGGTCCCTTC GTCACCATGA TCTTCTCTAT GATCACGGGT 
GACATGTTCA CCTTCGGCAT CATCTACATG ATCGTTCTGT TCGGATTTTC CCAGGCGTTC 
TACTTCCTGT ACAAAGGTCA TCCGGAAGCG GAGGATTCTC CATTTGGGAG CTACTTCGGC 
ACTTGGATGG GCCTGTTCCA GACAACCCTC GGCGATTACG ATTACGCCGA CTTGAATCTT 
ACGACCTACC CGAACCTGGC GAAAACCGTG TTTATCATAT TCATGATCTT CGTGCCGATC 
CTGTTGTTGA ACATGTTGAT CGCTATGATG GGCAACACCT ACGCGTACGT CATTGAGCAA 
GCTGAAAAGG AAGGCATGAA ACAGTGGGCC AAGATCGTGG TCAACCTGGA GCGGGCGGTC 
AAGCAGGAGG ACGCCAAAAA GTACCTGGAG GAGTACTCGA TCGGGTTGGG ACCGTCGGAT 
GATCCGAGGT ACGAGATCCG TGGAGTCATG GTCATCAAGA GCAAGAGTAA GACCCGTGCC 
AGGCAGCGCA AGGGAGCGGT AAGTAACTGG AAGAGCGTTC TGCGTGTCAC GTTGAACGAG 
CTGAAGAAGC GTAACATGAC CGGCGAGGAG CTGCGGAGGA TCATGTGGGG ACGATCGTCG 
ATCACTTCTC CGGCCAAGAT CTCCAAGAAG AAAAAGATCT ACGAAGAGGA GGACCCCTTC 
GCCATCACCA ATGCTATCGA TGTGATGTCC TTCACGCAGG ACATCGTCAT GGTAAGTAGC 
GAACCGACCG GACCGCCCAC AATCGACCCC TCCAAACCGG CGACGATCGC CGTCAAGCCC 
CAAACGGCAC CCGCAGCGCA ACCGATCCCG GAGATTCCTC CTCAACCGCC GCTGGTCAAA 
CGGCAGTCCG TGGTTAGTGC TCCGCCGGCC TACGACGATT TCCCCCCGAT GAAGGACTAC 
AAGGATCCCC TGCGGGAGCT GGTCATCATC TCGGAGTCGC CCTCGGTGGA CGACCATTAC 
GTGCAGAGCT GCAAGACCCT CGCGAATGAT GCGTCCACCC TGGATCACGT GCACGAGATC 
AACCTGACCA AGCAGAACCA GCTCCGGCAG GAGGAACAGA AGCAACAACA ACAGCAGACC 
TTGGGTCAGT TAAATCCCTT CCTGGAGGCG AAGGACGTGG TCGATCCGGT GAAGGAACGC 
GAGTTTCTCA AAACACTGGA GGCCCTGGAG GACACCGACA GCGAAGCGGC CGAAAAGCCG 
GTCCTGGGCA AGATCTCCCT GATACGACGC GCCAAGTCAG CCGTCTCGAG AACCACTTCC 
AGGAAGAAGA AAACCGACCA GCATCCGCTG TTCACGATCG CTTGGGATGA CAAGAACCTG 
ACCCGGACCC ATCCGGAGGA CTTTGGAGCG CTGAACACGG CCTACGAGTA CAGTGCCGAA 
GATTTCAAGC AAGACGTGGA CGAGGAAGCG AGGGAGGAGG AGGAAGACGA GGGTGTGACG 
GTGGAGGAAG TGCACCGCCG AATGGAAGAG CTGCACCATC GCGGCCGGGC TTCCTTGGAG 
CGGGACAGCA ACTCGACCGA GAGCAGCAAG AAACATCCGC ACAGGAAGCG GCAAGGTATG 
GGCCGAGGTC GGAACAACAA GGTGTCGCCG GATACGTCCA ACGAGAGCGT ATCCGGCAAG 
AAGGACAAAC GGATGAAGTC GGCGCCGACG GGTGGAGGTG GAGTTCGTCA TCGGCAGCTT 
ATGGACCAGG AAACGGGAGG GACCTCGCCA CCGGATCCAC TGGAACCATG GAGCACAAGG 
GATATTTGCA ACATTAATAA ACTTTTGGAC ACCGATACGC AGGATGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL009258 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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