AAEL_AAEL007705 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL007705
Entrez Gene ID	5569522
Full Name	E3 ubiquitin-protein ligase HECTD1
Synonyms	AAEL007705
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021848327.1	XP_021704019.1	E3 ubiquitin-protein ligase HECTD1 isoform X5
XM_021848324.1	XP_021704016.1	E3 ubiquitin-protein ligase HECTD1 isoform X2
XM_021848323.1	XP_021704015.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_021848322.1	XP_021704014.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_021848325.1	XP_021704017.1	E3 ubiquitin-protein ligase HECTD1 isoform X3
XM_021848326.1	XP_021704018.1	E3 ubiquitin-protein ligase HECTD1 isoform X4
XM_001652783.1	XP_001652833.1	AAEL007705-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL007705 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL007705 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe124649	XM_021848327.1 Latest version!	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OAe05212	XM_001652783.1 Latest version!	Aedes aegypti AAEL007705-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe124645	XM_021848323.1 Latest version!	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe124645	XM_021848322.1 Latest version!	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe124646	XM_021848324.1 Latest version!	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe124647	XM_021848325.1 Latest version!	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe124648	XM_021848326.1 Latest version!	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL007705 ( XM_021848327.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021704019.1 Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OAe124649
Clone ID Related Accession (Same CDS sequence)	XM_021848327.1
Accession Version	XM_021848327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACGT GCGGTGAAAA TCTGCATCTG CACCCGGACG ACGACAAAAG CAATAACAAC 
AACGACAAAC GCTGTTGTCG AAGCAATTGG TCCGATCTTA GCATTGCGGA CGACGAAGAT 
GGCCATCTGG TGGTGCTTAC CATTATCGAA GAGCTCATGT CCAAGACGCA AGACGATTTC 
CTCGATCACT TTGCCCGGCT CGGAGTGTTC TCCAAAGTTC AGGCCCTGAT GGGAGAAAAC 
GGCTTCAGCG GGGGCGAAGG TGATAACAAC GATGTGATCA AATCCCAAGA CGAGCTCAAA 
CCGTCTGCGA CCGGAGCACA AGATGCTTCG GTTGTCTCCA CTACTGGAAG CTCCTCCTCT 
GGAACTGCCA CTACTAATAC GGTGGAAGAT GCCAAAGAAA TCCTTCCCGG GAAGGCGTAC 
CACTGGCGAG ACTGGAGTAT TTGTCGAGGT CGCGACTGCT TGTACGTATG GTCGGATTCA 
GCCGCCTTGG AGCTTTCGAA CGGATCCAAT GGGTGGTTCC GGTTCATCCT AGACGGGAAA 
TTGGCCACTA TGTACTCCAG TGGTAGTCCA GAGAATGGCA GTGATAGCTC AGGCAAGGGA 
AGGACAGCGG ATTCCTTAGC CGGCGAAGAA AACCGTGGTG AATTCCTAGA AAAATTACAA 
CGAGCTCGCG GAGCGGTACG GCAGGGAACT CCCTCCCAGC CGATTCTTTC GTCGCCGAGC 
CTTTCGCGAA TCGTGGTCGG CAATTGGGTG CTGCAGAGCC AAAAAGAGCA TCAGCTACAT 
ATCAACAACT CCGAAGGTCA CCAGGTGACG ATCCTGCAGG ACGAACTGCC CGGATTCATC 
TTCGAGAGCA ATCGCGGCAC GAAGCATACT TTCACGGCGG AAACCACTCT AGGACCGGAC 
TTCGCCGCCG GATGGATCAA CACCAAGAAG AAGAAGATGC GTTCGAAAGC GGAGGCTCAG 
AAATATCAGG TTAAAAACAT CGCCCGAGAT CTGTACAACC GGTACTTCAA AGCAGCTCAA 
GCCGTCCCGC GGGGCGCCGT GGCAAAACTG TGCACCATCG TGCGTCAAAT CGAGGCAGCC 
CTGGAGGAAC AATGTGCCCC TAAGGCGTCC AGTTTGCTGC AGGTTCGGAT TCAACCTGAC 
GCTTCTAAAG CAACGAACAG CACATGGCAG GAGAAGCTGT ACAACGCTCT GCACGATCTT 
GTGCAACTGT TGAACGACGA TGGAGTCATC AGTGCTTACG AGATGCACAG TTCCGGACTT 
GTACAATCCT TGGTGGCCGT GCTGTCCCGG AACTACTGGG AACTGGAGAT GAACCGGAGC 
AAGGCCAACA AGTACCAGAA GCAACGGATA TCCATTTTCA AGAAGTGCAT GTATGGCGAT 
ACCAAAAATG GCAAAAACAC GGCCAGCATT CTGGTGCAGA AGTTGGTATC TGTATTGGAG 
AGCATTGAGA AGCTTCCGGT GCACATGTAT GACTCCCCAG GGGGTAGCTA TGGGTTGCAG 
ATTCTCACAA AGCGACTGAG CTTCCGACTT GAGCGGGCAG CTTGTGAGCA AACTCTCTTC 
GATAGGACCG GTAGAAACCT GAAAATGGAA CCATTGGCAA CCGTTGGCCA GCTGAACAAG 
TATCTTCTGA AAATGGTAGC AAAGCAGTGG TACGATATGG ATCGATCGTC CTTCTTGTAC 
TTGAAAAAGC TGAAAGAGGC AAAACCAGGA AGTGTACACT TCAAGCACCA GCACGATTTC 
GACGAGAATG GTCTCGTGTA CTATATCGGG ACGAACGGAA AAACCACGGA ATGGGTCAAT 
CCTGCTCAGT ACGGATTGGT TACTGTCACA AGTAGCGAAG GCAAACAGCT CCCGTACGGT 
AAGCTGGAAG ATATCCTGTC GAGAGATAGC GTTAGCGTGA ATTGTCACAC CAAGGATAAC 
AAGAAATCGT GGTTCGCCAT CGATTTGGGA ATGTTTATCA TTCCAACTGC TTATACTTTG 
CGACACGCCC GTGGCTATGG CCGCTCTGCT CTGAGAAATT GGATGTTCCA GATGTCAAAG 
GATGGAATAA ATTGGGCGAC AATGTTGACC CATTCGGATG ATAAAAGTTT GGCAGAACCC 
GGAAGCACTT GCACTTGGCC ACTCGAATGC TCTGCCGAAG AACAGCAGGG ATGGCGTCAC 
GTGAGAATCC ATCAAAATGG ACGGAATGCT TCAGGGCAGA CCCATTACCT TAGCTTGAGT 
GGATTTGAAA TCTACGGAAA GGTTGTCTCG GTTTGTGATG ATATGGGAAA AGCTGCGGCC 
AAGGAGAATG AAGCTCGGCT TAGGAAAGAG CGACGACAGA TTCGAGCTCA GTTGAAGTAT 
ATCACTCAAG GTGCACGCGT TGTTCGAGGA GTTGATTGGC ATTGGGATGA TCAAGATGGA 
AGTCCTCCTG GAGAAGGAAC CGTAACTGGT GAGATTCATA ACGGTTGGAT CGATGTAAAG 
TGGGACCATG GTTTAAGAAA CTCCTATCGC ATGGGAGCCG AAGGCAAATA TGATCTGAAG 
CTGTCAAACA GTGAAAACCT GACTGCACCT TACGATATGA ACAACTCCGG TGCTGGACTT 
GTTCCAATTA ACGCAGGAAG CGTGTCTTCG GCGAAGAAAG TATATGATAA GTCGTTGAAC 
GTTCTGACCA GTCGCAAATC GAGCTCTACT CCGAGCCTCC CGGAAGCAAC GGAGAACAAG 
TCATCAGTAG CTTCGACTGA ACAAGCCACT TCCGTGGATA ACCTTGCATG GAAACAAGCC 
GTAGAAGTGA TTGCCGAAAA TGTGCTATCC TGTGCTCGAT CTGATTTGGC CAATACCAGT 
GGTGGAAGTA GCAGCAATGA CTTATCCGCT CCCGGATCCA ACAATAATAA TCTGAACAAC 
CAGGAAGTGT CTGTGATTGT CCACTCGTTG GGAGAGCGGG GAAACATCCC AGATCTGTCG 
CAAATCAATA CAAGTACTTC GACTCTAGTC TCGGATTTGG CCACCATCAC GGAGAATCTA 
ACACTCTCGG ATAACATCAA GAACAACATC AGCATCGGAA GTAGCACCCA GTTTGTGAGC 
AACTTTGGAA CCCAACTTGC AGCTTGCTCA TCCTCGTCTT CGTCCGAAGA CAACAACAAA 
ACCAACAACA TCAATGAAAC CAATAACAAA ATCAATCTCA ACAACAGCAG CAGCAGTAGT 
TCCGGTAAAA CGGCATACCT GCCAACCAAG CTCGACGTGT TGGACAAAAT GAGGGAAGGC 
GTCGACATGC TTCGGAACAA TACCAACAAT TTGCTGTCCT CGGAATTGCT CACCCAGTCA 
AATCTACTCT CGTCCGTAAA GATTGCACTT CCGACGCCAC AACAACCGCA GCAAACTGGA 
GCGACAGCTC CCTCAGGACC TTTCTTCGTG GCCAGCACAA GCACTAGCAG CACGAGCACT 
CTCCCTTCGG GTGATAAAAT CGTCGGAGAT GTCAAGTTTA ACAATACCAT CAACAATAAT 
ACCGCTTCCG GTGGCGTAAC GAAGAAAGTC CTGAACGAAG TCAAACAGCA GGAACCGGAC 
GATCGCGACA TTGCCAACAA TTTGAAGAAC AACACCATTG TCGTCGATTC GGTGGAAGTT 
GGTGCAGCAG GAAGTTCGAA GGAATCAACT GCTGAGTCAC CTTCGGCGGC GGTCACGGTC 
AATCCGATGA GTGTGAGTGT CCCAAATCTG ACGAGCAATG ATAACAATGC TTCACAGGAG 
GTACAAACTC CACCAGGGTT GTTGGAAACG TTTGCGGCCA TAGCTCGAAG GCGTACTTCT 
GGAAGCTCTG TATCCCATCA TGTGAACCCT AGCTCAAACG CTTCAACCGC TTCTTCCGGA 
ACCAGTGGCA CAAACAATGC TCAACCCAAC AATCAGCTGA GTAGCCTTGG GTCCAATATT 
CAAGCAGCGA ACTCTAGCTT CTTCCCGAGG GGGCAAAACT CCGTTACTAG TCTGGTGAAA 
TTGGCCCTTT CGAGCAACTT CCACAGTGGA TTGTTGAGTA CCGCACAAAG TTATCCTAGT 
CTCTCGAGTT CGAGTTCGAA TAATGCAAAT TCGGTCGCGG TAAGTGGAGG AACCAACAAC 
AATTCAGCGA GCGGAGTCGG TTCCAACAAT GGATCCGTTC AAAGCGGAAA TGTCCAAGCC 
GTGTTGAATC CAGCACTGAC GATGAGTCTC ACCTCAACCA GTAGCGATAG TGAGCAGGTG 
TCTTTGGAAG ATTTCCTTGA ACAGTGCCGA GCTCCAACAT TGCTGGGAGA TTTAGACGAC 
GACGAAGACA TGGAGGACGA GAATGATGAC GATGAAAATG AGGATGAATA CGAAGAAGTC 
GGAAATACTT TGCTTCAAGT GATGGTTTCT CGTAATTTGT TATCGTTTAT GGATGAGGAG 
ACTTTGGAGA ATCGTCTGGC TGCCGCTGGC AAACGCAAAT CGTGGGACGA CGAATTCGTG 
CTTAAACGCC AATTCTCGGC ACTCATTCCT GCATTCGATC CGCGTCCTGG CAGGACCAAC 
GTCAATCAAA CAAGTGATCT GGAAATACCT GCTCCCGGCA GTTCTACAGA CAGTAGTCAT 
CCGAGTAGCA GCCACTCTGA GCATGCTCCT TTGCCTCAAC CGTCCCTTGC ACTTCTTCTG 
CGTGGTCCCA ACATAAACGG AGTAAATGAT GTGGAAATTC CACTGTCGCA GCCCGATTGG 
ACAATATTCC GAGCGGTCCA AGAACTGATC CTTCAAACCA ACATGACCAA GCAGGATAAG 
TTCCGCAAAA TTTGGCAACC AACGTACACC ATCGTCTACA GGGAAGCCTC TTCTTCGGCC 
GGACTCGGTT CCTGCCGCGG TGAGGACTTC AGCAGCGGAG AAGAAGGACG GGCCACCCCC 
GTCGTTTCCA TGTTTTCCCA ACGCAGTGGA GGATCTACCC TTTCGCCTAG CTCTCCGATC 
CCCGGAACAC CACTCAATCC AACCGCCACC GCTCACTGCA CTGTGGACGA CGTCTTGCAG 
CTTCTCGGCC AATTGAATGC CATCAATCGA ACGCTTACAT CTTCTCCATC CAACAACGAC 
AAGAACCTCA TCCCGGACAT GGAATCCAAC AATCTCAACG CGGATATATT CTTGAGCAAG 
AAAATTACCA ACAAACTGCA GCAGCAAATC CAAGACCCAC TAGTCCTGGC GAGTGGAAGC 
CTGCCGAAAT GGTGCGAAGA CTTCAACCAA AGCTGTCCGT TCCTGTTCCC GTTCGAGACG 
CGGCAGCTGT ACTTCAACTG TACGGCTTTC GGCGCCTCGA GGAGCATCGT GTGGCTGCAG 
TCGCAGCGGG ATGTGAACCT GGAACGGCAA CGGGCTCCCG GGTTGAGCCC ACGGCATGCC 
GATCAGCACG AGTTCCGCGT TGGGCGGTTG AAGCACGAGC GCGTCAAGGT TCCACGCGGT 
GAGAATCTAC TCGAATGGGC ACAACAGGTT ATGAAAGTTC ACTGTAATCG CAAGTCGGTG 
TTGGAGGTGG AATTCGTTGG AGAGGAAGGC ACTGGATTGG GTCCGACTTT GGAATTCTAC 
GCCCTGGTTG CAGCTGAACT GCAGCGAAGT GATCTGGGAA TGTGGCTGTG CGACGATGAA 
CCTAAGCTAA TCGAAGACGA AATCGATCTA GGAGAGGGAA GCAAACCCAT CGGTTATTAC 
GTTCGTCGTT CAACCGGATT GTTCCCGGCG CCGTTGCCAC AGGAATCTGA AATTTGTGAC 
TTTGTGTCAA ACTACTTCTG GTTCTTGGGA GTGTTTCTGG CGAAGGTACT GCAGGACGGT 
CGTCTAGTCG ATCTGCCACT GTCGAACAGC TTCTTACAAC TGTTATGTAA TAACAAATCT 
CTCGCTAGAG GTTCACTAAG CGAACTTTCT TCCAAGTCTG CCTTGCACGA TGATGTGATG 
ATTTCTTCTT TAATGTCCGA GGAAAGCGAT CGTGATCTGG TGGATTCATA TCAGTCAAAA 
TTGGCTAACG ATGGCTGTTG GTATGATGGA ATCCTGTCCC AAGAAAATCT CCAAGAAATC 
GATCCGATTC GGTACGAGTT CTTGAAAGAA CTGCAAGAGT TGGTGCAGCA GAAACAAAAT 
ATCGAACAAA ATGATGATCT CACCTCCGAA GAGAAGCTAC TGCAAATTAG CGAGCTTAAA 
TTCAACACCA AAACTGGCAG TGTAGCTTTG GAGGACCTCG CTCTAACGTT CACCTATCTA 
CCAAGTTCGA AGAACTACGG CTACCAATCG GCAGATCTGA TTCCAAACGG TTCCAACATT 
GATGTAACGA TCAACAACGT CGAAGAGTAC TGCAATTTGA CGATCAACTT CTGCCTGCAA 
GAAGGCATTG CTAAGCAACT TACTGCGTTC CATCGAGGTT TCTGTGAAGT GTTCCCGCTG 
AACAAATTGG CCGCTTTCAC ATCGGAAGAA ATCCGCAAGA TGCTTTGTGG CGAGCAGAAC 
CCAGAGTGGA CACGGGAGGA CATTATGACC TACACGGAAC CGAAACTCGG ATACTCCAAG 
GAAAGCCCTG GATTCCTGCG TTTCGTGAAC GTGTTGATGG GGATGAATGC CAGCGAAAGG 
AAAGCATTTT TGCAGTTCAC CACCGGCTGC AGCAGTTTAC CACCCGGTGG GCTGGCCAAT 
TTGCACCCGC GGCTGACAGT GGTGCGGAAG GTAGATGCCG GCGAAGGTTC CTACCCTTCG 
GTCAACACCT GTGTCCACTA CCTCAAATTG CCGGACTATC CGAATGAGGA AATCTTGCGG 
GAACGATTGC TGACGGCCAC AAAAGAAAAG GGATTCCATC TTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021704019.1
CDS	1..6768
Translation	MATCGENLHLHPDDDKSNNNNDKRCCRSNWSDLSIADDEDGHLVVLTIIEELMSKTQDDFLDHFARLGVFSKVQALMGENGFSGGEGDNNDVIKSQDELKPSATGAQDASVVSTTGSSSSGTATTNTVEDAKEILPGKAYHWRDWSICRGRDCLYVWSDSAALELSNGSNGWFRFILDGKLATMYSSGSPENGSDSSGKGRTADSLAGEENRGEFLEKLQRARGAVRQGTPSQPILSSPSLSRIVVGNWVLQSQKEHQLHINNSEGHQVTILQDELPGFIFESNRGTKHTFTAETTLGPDFAAGWINTKKKKMRSKAEAQKYQVKNIARDLYNRYFKAAQAVPRGAVAKLCTIVRQIEAALEEQCAPKASSLLQVRIQPDASKATNSTWQEKLYNALHDLVQLLNDDGVISAYEMHSSGLVQSLVAVLSRNYWELEMNRSKANKYQKQRISIFKKCMYGDTKNGKNTASILVQKLVSVLESIEKLPVHMYDSPGGSYGLQILTKRLSFRLERAACEQTLFDRTGRNLKMEPLATVGQLNKYLLKMVAKQWYDMDRSSFLYLKKLKEAKPGSVHFKHQHDFDENGLVYYIGTNGKTTEWVNPAQYGLVTVTSSEGKQLPYGKLEDILSRDSVSVNCHTKDNKKSWFAIDLGMFIIPTAYTLRHARGYGRSALRNWMFQMSKDGINWATMLTHSDDKSLAEPGSTCTWPLECSAEEQQGWRHVRIHQNGRNASGQTHYLSLSGFEIYGKVVSVCDDMGKAAAKENEARLRKERRQIRAQLKYITQGARVVRGVDWHWDDQDGSPPGEGTVTGEIHNGWIDVKWDHGLRNSYRMGAEGKYDLKLSNSENLTAPYDMNNSGAGLVPINAGSVSSAKKVYDKSLNVLTSRKSSSTPSLPEATENKSSVASTEQATSVDNLAWKQAVEVIAENVLSCARSDLANTSGGSSSNDLSAPGSNNNNLNNQEVSVIVHSLGERGNIPDLSQINTSTSTLVSDLATITENLTLSDNIKNNISIGSSTQFVSNFGTQLAACSSSSSSEDNNKTNNINETNNKINLNNSSSSSSGKTAYLPTKLDVLDKMREGVDMLRNNTNNLLSSELLTQSNLLSSVKIALPTPQQPQQTGATAPSGPFFVASTSTSSTSTLPSGDKIVGDVKFNNTINNNTASGGVTKKVLNEVKQQEPDDRDIANNLKNNTIVVDSVEVGAAGSSKESTAESPSAAVTVNPMSVSVPNLTSNDNNASQEVQTPPGLLETFAAIARRRTSGSSVSHHVNPSSNASTASSGTSGTNNAQPNNQLSSLGSNIQAANSSFFPRGQNSVTSLVKLALSSNFHSGLLSTAQSYPSLSSSSSNNANSVAVSGGTNNNSASGVGSNNGSVQSGNVQAVLNPALTMSLTSTSSDSEQVSLEDFLEQCRAPTLLGDLDDDEDMEDENDDDENEDEYEEVGNTLLQVMVSRNLLSFMDEETLENRLAAAGKRKSWDDEFVLKRQFSALIPAFDPRPGRTNVNQTSDLEIPAPGSSTDSSHPSSSHSEHAPLPQPSLALLLRGPNINGVNDVEIPLSQPDWTIFRAVQELILQTNMTKQDKFRKIWQPTYTIVYREASSSAGLGSCRGEDFSSGEEGRATPVVSMFSQRSGGSTLSPSSPIPGTPLNPTATAHCTVDDVLQLLGQLNAINRTLTSSPSNNDKNLIPDMESNNLNADIFLSKKITNKLQQQIQDPLVLASGSLPKWCEDFNQSCPFLFPFETRQLYFNCTAFGASRSIVWLQSQRDVNLERQRAPGLSPRHADQHEFRVGRLKHERVKVPRGENLLEWAQQVMKVHCNRKSVLEVEFVGEEGTGLGPTLEFYALVAAELQRSDLGMWLCDDEPKLIEDEIDLGEGSKPIGYYVRRSTGLFPAPLPQESEICDFVSNYFWFLGVFLAKVLQDGRLVDLPLSNSFLQLLCNNKSLARGSLSELSSKSALHDDVMISSLMSEESDRDLVDSYQSKLANDGCWYDGILSQENLQEIDPIRYEFLKELQELVQQKQNIEQNDDLTSEEKLLQISELKFNTKTGSVALEDLALTFTYLPSSKNYGYQSADLIPNGSNIDVTINNVEEYCNLTINFCLQEGIAKQLTAFHRGFCEVFPLNKLAAFTSEEIRKMLCGEQNPEWTREDIMTYTEPKLGYSKESPGFLRFVNVLMGMNASERKAFLQFTTGCSSLPPGGLANLHPRLTVVRKVDAGEGSYPSVNTCVHYLKLPDYPNEEILRERLLTATKEKGFHLN

Target ORF information:

RefSeq Version	XM_021848327.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848327.1

ORF Insert Sequence:

ATGGCAACGT GCGGTGAAAA TCTGCATCTG CACCCGGACG ACGACAAAAG CAATAACAAC 
AACGACAAAC GCTGTTGTCG AAGCAATTGG TCCGATCTTA GCATTGCGGA CGACGAAGAT 
GGCCATCTGG TGGTGCTTAC CATTATCGAA GAGCTCATGT CCAAGACGCA AGACGATTTC 
CTCGATCACT TTGCCCGGCT CGGAGTGTTC TCCAAAGTTC AGGCCCTGAT GGGAGAAAAC 
GGCTTCAGCG GGGGCGAAGG TGATAACAAC GATGTGATCA AATCCCAAGA CGAGCTCAAA 
CCGTCTGCGA CCGGAGCACA AGATGCTTCG GTTGTCTCCA CTACTGGAAG CTCCTCCTCT 
GGAACTGCCA CTACTAATAC GGTGGAAGAT GCCAAAGAAA TCCTTCCCGG GAAGGCGTAC 
CACTGGCGAG ACTGGAGTAT TTGTCGAGGT CGCGACTGCT TGTACGTATG GTCGGATTCA 
GCCGCCTTGG AGCTTTCGAA CGGATCCAAT GGGTGGTTCC GGTTCATCCT AGACGGGAAA 
TTGGCCACTA TGTACTCCAG TGGTAGTCCA GAGAATGGCA GTGATAGCTC AGGCAAGGGA 
AGGACAGCGG ATTCCTTAGC CGGCGAAGAA AACCGTGGTG AATTCCTAGA AAAATTACAA 
CGAGCTCGCG GAGCGGTACG GCAGGGAACT CCCTCCCAGC CGATTCTTTC GTCGCCGAGC 
CTTTCGCGAA TCGTGGTCGG CAATTGGGTG CTGCAGAGCC AAAAAGAGCA TCAGCTACAT 
ATCAACAACT CCGAAGGTCA CCAGGTGACG ATCCTGCAGG ACGAACTGCC CGGATTCATC 
TTCGAGAGCA ATCGCGGCAC GAAGCATACT TTCACGGCGG AAACCACTCT AGGACCGGAC 
TTCGCCGCCG GATGGATCAA CACCAAGAAG AAGAAGATGC GTTCGAAAGC GGAGGCTCAG 
AAATATCAGG TTAAAAACAT CGCCCGAGAT CTGTACAACC GGTACTTCAA AGCAGCTCAA 
GCCGTCCCGC GGGGCGCCGT GGCAAAACTG TGCACCATCG TGCGTCAAAT CGAGGCAGCC 
CTGGAGGAAC AATGTGCCCC TAAGGCGTCC AGTTTGCTGC AGGTTCGGAT TCAACCTGAC 
GCTTCTAAAG CAACGAACAG CACATGGCAG GAGAAGCTGT ACAACGCTCT GCACGATCTT 
GTGCAACTGT TGAACGACGA TGGAGTCATC AGTGCTTACG AGATGCACAG TTCCGGACTT 
GTACAATCCT TGGTGGCCGT GCTGTCCCGG AACTACTGGG AACTGGAGAT GAACCGGAGC 
AAGGCCAACA AGTACCAGAA GCAACGGATA TCCATTTTCA AGAAGTGCAT GTATGGCGAT 
ACCAAAAATG GCAAAAACAC GGCCAGCATT CTGGTGCAGA AGTTGGTATC TGTATTGGAG 
AGCATTGAGA AGCTTCCGGT GCACATGTAT GACTCCCCAG GGGGTAGCTA TGGGTTGCAG 
ATTCTCACAA AGCGACTGAG CTTCCGACTT GAGCGGGCAG CTTGTGAGCA AACTCTCTTC 
GATAGGACCG GTAGAAACCT GAAAATGGAA CCATTGGCAA CCGTTGGCCA GCTGAACAAG 
TATCTTCTGA AAATGGTAGC AAAGCAGTGG TACGATATGG ATCGATCGTC CTTCTTGTAC 
TTGAAAAAGC TGAAAGAGGC AAAACCAGGA AGTGTACACT TCAAGCACCA GCACGATTTC 
GACGAGAATG GTCTCGTGTA CTATATCGGG ACGAACGGAA AAACCACGGA ATGGGTCAAT 
CCTGCTCAGT ACGGATTGGT TACTGTCACA AGTAGCGAAG GCAAACAGCT CCCGTACGGT 
AAGCTGGAAG ATATCCTGTC GAGAGATAGC GTTAGCGTGA ATTGTCACAC CAAGGATAAC 
AAGAAATCGT GGTTCGCCAT CGATTTGGGA ATGTTTATCA TTCCAACTGC TTATACTTTG 
CGACACGCCC GTGGCTATGG CCGCTCTGCT CTGAGAAATT GGATGTTCCA GATGTCAAAG 
GATGGAATAA ATTGGGCGAC AATGTTGACC CATTCGGATG ATAAAAGTTT GGCAGAACCC 
GGAAGCACTT GCACTTGGCC ACTCGAATGC TCTGCCGAAG AACAGCAGGG ATGGCGTCAC 
GTGAGAATCC ATCAAAATGG ACGGAATGCT TCAGGGCAGA CCCATTACCT TAGCTTGAGT 
GGATTTGAAA TCTACGGAAA GGTTGTCTCG GTTTGTGATG ATATGGGAAA AGCTGCGGCC 
AAGGAGAATG AAGCTCGGCT TAGGAAAGAG CGACGACAGA TTCGAGCTCA GTTGAAGTAT 
ATCACTCAAG GTGCACGCGT TGTTCGAGGA GTTGATTGGC ATTGGGATGA TCAAGATGGA 
AGTCCTCCTG GAGAAGGAAC CGTAACTGGT GAGATTCATA ACGGTTGGAT CGATGTAAAG 
TGGGACCATG GTTTAAGAAA CTCCTATCGC ATGGGAGCCG AAGGCAAATA TGATCTGAAG 
CTGTCAAACA GTGAAAACCT GACTGCACCT TACGATATGA ACAACTCCGG TGCTGGACTT 
GTTCCAATTA ACGCAGGAAG CGTGTCTTCG GCGAAGAAAG TATATGATAA GTCGTTGAAC 
GTTCTGACCA GTCGCAAATC GAGCTCTACT CCGAGCCTCC CGGAAGCAAC GGAGAACAAG 
TCATCAGTAG CTTCGACTGA ACAAGCCACT TCCGTGGATA ACCTTGCATG GAAACAAGCC 
GTAGAAGTGA TTGCCGAAAA TGTGCTATCC TGTGCTCGAT CTGATTTGGC CAATACCAGT 
GGTGGAAGTA GCAGCAATGA CTTATCCGCT CCCGGATCCA ACAATAATAA TCTGAACAAC 
CAGGAAGTGT CTGTGATTGT CCACTCGTTG GGAGAGCGGG GAAACATCCC AGATCTGTCG 
CAAATCAATA CAAGTACTTC GACTCTAGTC TCGGATTTGG CCACCATCAC GGAGAATCTA 
ACACTCTCGG ATAACATCAA GAACAACATC AGCATCGGAA GTAGCACCCA GTTTGTGAGC 
AACTTTGGAA CCCAACTTGC AGCTTGCTCA TCCTCGTCTT CGTCCGAAGA CAACAACAAA 
ACCAACAACA TCAATGAAAC CAATAACAAA ATCAATCTCA ACAACAGCAG CAGCAGTAGT 
TCCGGTAAAA CGGCATACCT GCCAACCAAG CTCGACGTGT TGGACAAAAT GAGGGAAGGC 
GTCGACATGC TTCGGAACAA TACCAACAAT TTGCTGTCCT CGGAATTGCT CACCCAGTCA 
AATCTACTCT CGTCCGTAAA GATTGCACTT CCGACGCCAC AACAACCGCA GCAAACTGGA 
GCGACAGCTC CCTCAGGACC TTTCTTCGTG GCCAGCACAA GCACTAGCAG CACGAGCACT 
CTCCCTTCGG GTGATAAAAT CGTCGGAGAT GTCAAGTTTA ACAATACCAT CAACAATAAT 
ACCGCTTCCG GTGGCGTAAC GAAGAAAGTC CTGAACGAAG TCAAACAGCA GGAACCGGAC 
GATCGCGACA TTGCCAACAA TTTGAAGAAC AACACCATTG TCGTCGATTC GGTGGAAGTT 
GGTGCAGCAG GAAGTTCGAA GGAATCAACT GCTGAGTCAC CTTCGGCGGC GGTCACGGTC 
AATCCGATGA GTGTGAGTGT CCCAAATCTG ACGAGCAATG ATAACAATGC TTCACAGGAG 
GTACAAACTC CACCAGGGTT GTTGGAAACG TTTGCGGCCA TAGCTCGAAG GCGTACTTCT 
GGAAGCTCTG TATCCCATCA TGTGAACCCT AGCTCAAACG CTTCAACCGC TTCTTCCGGA 
ACCAGTGGCA CAAACAATGC TCAACCCAAC AATCAGCTGA GTAGCCTTGG GTCCAATATT 
CAAGCAGCGA ACTCTAGCTT CTTCCCGAGG GGGCAAAACT CCGTTACTAG TCTGGTGAAA 
TTGGCCCTTT CGAGCAACTT CCACAGTGGA TTGTTGAGTA CCGCACAAAG TTATCCTAGT 
CTCTCGAGTT CGAGTTCGAA TAATGCAAAT TCGGTCGCGG TAAGTGGAGG AACCAACAAC 
AATTCAGCGA GCGGAGTCGG TTCCAACAAT GGATCCGTTC AAAGCGGAAA TGTCCAAGCC 
GTGTTGAATC CAGCACTGAC GATGAGTCTC ACCTCAACCA GTAGCGATAG TGAGCAGGTG 
TCTTTGGAAG ATTTCCTTGA ACAGTGCCGA GCTCCAACAT TGCTGGGAGA TTTAGACGAC 
GACGAAGACA TGGAGGACGA GAATGATGAC GATGAAAATG AGGATGAATA CGAAGAAGTC 
GGAAATACTT TGCTTCAAGT GATGGTTTCT CGTAATTTGT TATCGTTTAT GGATGAGGAG 
ACTTTGGAGA ATCGTCTGGC TGCCGCTGGC AAACGCAAAT CGTGGGACGA CGAATTCGTG 
CTTAAACGCC AATTCTCGGC ACTCATTCCT GCATTCGATC CGCGTCCTGG CAGGACCAAC 
GTCAATCAAA CAAGTGATCT GGAAATACCT GCTCCCGGCA GTTCTACAGA CAGTAGTCAT 
CCGAGTAGCA GCCACTCTGA GCATGCTCCT TTGCCTCAAC CGTCCCTTGC ACTTCTTCTG 
CGTGGTCCCA ACATAAACGG AGTAAATGAT GTGGAAATTC CACTGTCGCA GCCCGATTGG 
ACAATATTCC GAGCGGTCCA AGAACTGATC CTTCAAACCA ACATGACCAA GCAGGATAAG 
TTCCGCAAAA TTTGGCAACC AACGTACACC ATCGTCTACA GGGAAGCCTC TTCTTCGGCC 
GGACTCGGTT CCTGCCGCGG TGAGGACTTC AGCAGCGGAG AAGAAGGACG GGCCACCCCC 
GTCGTTTCCA TGTTTTCCCA ACGCAGTGGA GGATCTACCC TTTCGCCTAG CTCTCCGATC 
CCCGGAACAC CACTCAATCC AACCGCCACC GCTCACTGCA CTGTGGACGA CGTCTTGCAG 
CTTCTCGGCC AATTGAATGC CATCAATCGA ACGCTTACAT CTTCTCCATC CAACAACGAC 
AAGAACCTCA TCCCGGACAT GGAATCCAAC AATCTCAACG CGGATATATT CTTGAGCAAG 
AAAATTACCA ACAAACTGCA GCAGCAAATC CAAGACCCAC TAGTCCTGGC GAGTGGAAGC 
CTGCCGAAAT GGTGCGAAGA CTTCAACCAA AGCTGTCCGT TCCTGTTCCC GTTCGAGACG 
CGGCAGCTGT ACTTCAACTG TACGGCTTTC GGCGCCTCGA GGAGCATCGT GTGGCTGCAG 
TCGCAGCGGG ATGTGAACCT GGAACGGCAA CGGGCTCCCG GGTTGAGCCC ACGGCATGCC 
GATCAGCACG AGTTCCGCGT TGGGCGGTTG AAGCACGAGC GCGTCAAGGT TCCACGCGGT 
GAGAATCTAC TCGAATGGGC ACAACAGGTT ATGAAAGTTC ACTGTAATCG CAAGTCGGTG 
TTGGAGGTGG AATTCGTTGG AGAGGAAGGC ACTGGATTGG GTCCGACTTT GGAATTCTAC 
GCCCTGGTTG CAGCTGAACT GCAGCGAAGT GATCTGGGAA TGTGGCTGTG CGACGATGAA 
CCTAAGCTAA TCGAAGACGA AATCGATCTA GGAGAGGGAA GCAAACCCAT CGGTTATTAC 
GTTCGTCGTT CAACCGGATT GTTCCCGGCG CCGTTGCCAC AGGAATCTGA AATTTGTGAC 
TTTGTGTCAA ACTACTTCTG GTTCTTGGGA GTGTTTCTGG CGAAGGTACT GCAGGACGGT 
CGTCTAGTCG ATCTGCCACT GTCGAACAGC TTCTTACAAC TGTTATGTAA TAACAAATCT 
CTCGCTAGAG GTTCACTAAG CGAACTTTCT TCCAAGTCTG CCTTGCACGA TGATGTGATG 
ATTTCTTCTT TAATGTCCGA GGAAAGCGAT CGTGATCTGG TGGATTCATA TCAGTCAAAA 
TTGGCTAACG ATGGCTGTTG GTATGATGGA ATCCTGTCCC AAGAAAATCT CCAAGAAATC 
GATCCGATTC GGTACGAGTT CTTGAAAGAA CTGCAAGAGT TGGTGCAGCA GAAACAAAAT 
ATCGAACAAA ATGATGATCT CACCTCCGAA GAGAAGCTAC TGCAAATTAG CGAGCTTAAA 
TTCAACACCA AAACTGGCAG TGTAGCTTTG GAGGACCTCG CTCTAACGTT CACCTATCTA 
CCAAGTTCGA AGAACTACGG CTACCAATCG GCAGATCTGA TTCCAAACGG TTCCAACATT 
GATGTAACGA TCAACAACGT CGAAGAGTAC TGCAATTTGA CGATCAACTT CTGCCTGCAA 
GAAGGCATTG CTAAGCAACT TACTGCGTTC CATCGAGGTT TCTGTGAAGT GTTCCCGCTG 
AACAAATTGG CCGCTTTCAC ATCGGAAGAA ATCCGCAAGA TGCTTTGTGG CGAGCAGAAC 
CCAGAGTGGA CACGGGAGGA CATTATGACC TACACGGAAC CGAAACTCGG ATACTCCAAG 
GAAAGCCCTG GATTCCTGCG TTTCGTGAAC GTGTTGATGG GGATGAATGC CAGCGAAAGG 
AAAGCATTTT TGCAGTTCAC CACCGGCTGC AGCAGTTTAC CACCCGGTGG GCTGGCCAAT 
TTGCACCCGC GGCTGACAGT GGTGCGGAAG GTAGATGCCG GCGAAGGTTC CTACCCTTCG 
GTCAACACCT GTGTCCACTA CCTCAAATTG CCGGACTATC CGAATGAGGA AATCTTGCGG 
GAACGATTGC TGACGGCCAC AAAAGAAAAG GGATTCCATC TTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL007705 ( XM_001652783.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001652833.1 Aedes aegypti AAEL007705-RA partial mRNA.

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CloneID	OAe05212
Clone ID Related Accession (Same CDS sequence)	XM_001652783.1
Accession Version	XM_001652783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL007705-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810990). COMPLETENESS: incomplete on the 5' end.

ATGACATCTG AAACGAGAGC AGCTTGCCAC AGGAAAACAA ACCGTCCAAC AGTGTCGCTC 
TCGTCTCGTT CAGACGACGT TCGAATGGGT GACGTTGATC CAGAAACGCT GCTAGAATGG 
CTGTCGATGG GGCAGGGAGA CGAACGGGAT ATGCAGTTGA TTGCCCTGGA GCAGCTATGT 
ATGCTTCTGC TGATGTCCGA CAACGTTGAT CGTTGTTTCG AAAGTTGTCC CCCGCGAACG 
TTCCTCCCAG CGCTGTGTAA AATATTTTTA GATGAACTAG CGCCAGAGAA TGTACTGGAA 
GTGACAGCAC GTGCCATCAC CTACTATCTA GACGTATCAT CCGAGTGCAC ACGTCGCATT 
GTAGCGATCG ATGGTGCGAT CAAAGCTATC TGCAACCGGT TGGTGGTGGC CGATCTCGAA 
AGCAGAACTA GTCGTGATCT TGCGGAGCAG TGCATCAAAG TACTTGAGCT CATATGCACT 
CGAGAAGCAG GGGCCGTGTT TGAAGGAGGT GGACTTAATT GTGTGCTTTC ATTCATTCGG 
GACAATGGGT CTCAGATCCA CAAAGACACA CTCCACTCGG CAATGGCCGT GGTGTCCAGG 
CTATGTACCA AGGTGGAGCC ACAAGGTGCT AATGTACAAA CTTGTGTAGA AAGTCTAAGT 
ACTCTGCTAC AGCACGAAGA TCCGCTCGTA GCAGACGGAG CATTGAAATG CTTTGCTTCG 
GTGGCAGATC GATTTACTAG AAAAGGAGTT GATCCTGCCC CACTTGCAGA GTATGGACTA 
GTAACTGAGT TGTTAAATCG ACTAAGCAAC GCTGCAGGAC CACAAACATC AGCCTTGACA 
GCCGGAGCTA GTTCAAATGC GACGCCCGCT GCACAGGTCA ACAGTCAAGA GACCACAGGA 
ACAGTGCAGC TTTCATCGTC AGCACCAAAA ACACAGGGAG CAGCCGAAGC AGGGCGATCG 
AGTCAATCGA TCGCCACAAC TATATCACTG TTATCTACCT TGTGTCGAGG GTCACCATCG 
ATCACGCATG ATCTCCTCCG ATCCAATCTA CCAGATGCTA TGGAACGAGC CTTCAAAGGT 
GATGAACGAT GTGTTCTTGA CTGTATGAGG CTTGCCGATC TCATCCTGCT GCTGTTGTTC 
GAAGGTCGAC AAGCTCTTGG CAGGGTGGGT GGATCTAGCG GTCAGTTAGC TCCTCGTGTG 
CGAAGAGCAG ACTCTAGTAC TGAGCGAACA CATCGACAGT TGATCGATTG CATACGTAGT 
AAGGACACCG AAGCCCTAAT CGAAGCTATC GAATCGGGTG GAATCGATGT CAACTGCATG 
GACGATGTCG GTCAAACATT ACTTAACTGG GCTTCGGCCT TTGGTACATT AGAAATGGTA 
GAGTTTTTAT GTGATAAAGG TGCTGACGTC AACAAAGGCC AGCGAAGTTC CTCTCTGCAT 
TATGCGGCCT GTTTCGGTCG ACCCGGCATA GCAAAAGTAC TGCTCAAACA CGGTGCCAAT 
CCTGACCTAC GGGACGAAGA CGGCAAAACA CCACTGGATA AGGCTCGCGA ACGGCCGGAT 
GAAGGCCACC GTGAAGTTGC TTCCATTCTT CAGTCGCCTG GCGAATGGAT GACCGCGGCA 
ACACGATCGG ACTTGAAAAC CGACCCCGAA GACGGGGAAA GCGAGCCCCG CGGAGATCCG 
GAGATGGCAC CCGTCTATCT CAAGTTCTTT CTACCAATAT TCTGCAAGAC GTTCCAAAGC 
ACAATGCTAG CCAGCGTTCG ACGGTCTAGT TTAGGTTTGA TCAAGAAGAT GATTCAATAT 
GTCCAACCAG AAATGCTGTC GAGGCTTTGT TCCTCGGAGG GCCTCCAAAG TCATGAGCAC 
AGCCTAGGAA CTTTACTAGT CGAAGTCGTC GCCAGTGTTT TGGACAACGA GGACGACGAA 
GATGGCCATC TGGTGGTGCT TACCATTATC GAAGAGCTCA TGTCCAAGAC GCAAGACGAT 
TTCCTCGATC ACTTTGCCCG GCTCGGAGTG TTCTCCAAAG TGCAGGCCCT GATGGGAGAA 
AACGGCTTCA GCGGGGGCGA AGGTGATAAC AACGATGTGA TCAAATCCCA AGACGAGCTC 
AAACCGTCTG CGACCGGAGC ACAAGATGCC TCGGTTGTCT CCACTACTGG AAGCTCCTCC 
TCTGGAACTG CCACTACTAA TACGGTGGAA GATGCCAAAG AAATCCTTCC CGGGAAGGCG 
TACCACTGGC GAGACTGGAG TATTTGTCGA GGTCGCGACT GCTTGTACGT GTGGTCGGAT 
TCAGCCGCCT TGGAGCTTTC GAACGGATCC AATGGGTGGT TCCGGTTCAT CCTGGACGGG 
AAATTGGCCA CTATGTACTC CAGTGGTAGT CCAGAGAATG GCAGTGATAG CTCAGAAAAC 
CGTGGTGAAT TCCTAGAAAA ATTACAACGA GCTCGCGGAG CGGTACGGCA GGGAACTCCC 
TCCCAGCCGA TTCTTTCGTC GCCGAGCCTT TCGCGAATCG TGGTCGGCAA TTGGGTGCTG 
CAGAGCCAAA AAGAGCATCA GCTACATATC AACAACTCCG AAGGTCACCA GGTGACGATC 
CTGCAGGACG AACTGCCCGG ATTCATCTTC GAGAGCAATC GCGGCACGAA GCATACTTTC 
ACGGCGGAAA CCACTCTGGG ACCGGACTTC GCCGCCGGAT GGATCAACAC CAAGAAGAAG 
AAGATGCGTT CGAAGGCGGA GGCTCAGAAA TATCAGGTTA AAAACATCGC CCGAGATCTG 
TACAACCGGT ATTTCAAAGC AGCTCAAGCC GTCCCGCGGG GCGCCGTGGC AAAACTGTGC 
ACCATCGTGC GTCAAATCGA GGCAGCCCTG GAGGAACAAT GTGCCCCTAA GGCGTCCAGT 
TTGCTGCAGG TTCGGATTCA ACCTGACGCT TCTAAAGCAA CGAACAGTAC ATGGCAGGAG 
AAGCTGTACA ACGCTCTGCA CGATCTGGTG CAACTGTTGA ACGACGATGG AGTCATCAGT 
GCTTACGAGA TGCACAGTTC CGGACTTGTA CAATCCTTGG TGGCCGTCCT GTCCCGGAAC 
TACTGGGAAC TCGAAATGAA CCGGAGCAAG GCCAACAAGT ACCAGAAACA GCGGATATCC 
ATTTTCAAAA AGTGCATGTA TGGCGATACC AAAAATGGCA AAAACACGGC CAGCATTCTG 
GTGCAGAAGT TGGTATCTGT ATTGGAGAGC ATTGAGAAGC TTCCGGTGCA CATGTATGAC 
TCCCCAGGGG GTAGCTATGG GTTGCAGATT CTCACGAAGC GATTGAGCTT CCGACTTGAG 
CGGGCAGCTT GTGAGCAAAC TCTCTTCGAT AGGACCGGTA GAAACCTGAA AATGGAACCA 
TTGGCAACCG TTGGCCAGCT GAACAAGTAT CTTCTGAAAA TGGTAGCAAA GCAGTGGTAC 
GATATGGATC GATCGTCCTT CTTGTACTTG AAAAAGCTGA AAGAGGCAAA ACCAGGAAGT 
GTACACTTCA AGCACCAGCA CGATTTCGAC GAGAATGGTC TTGTGTACTA TATCGGGACG 
AACGGAAAAA CCACGGAATG GGTCAATCCT GCTCAGTACG GATTGGTTAC TGTCACAAGT 
AGCGAAGGCA AACAGCTCCC GTACGGTAAG CTGGAAGATA TCCTGTCGAG AGATAGCGTT 
AGCGTGAATT GTCACACCAA GGATAACAAG AAATCGTGGT TCGCCATCGA TTTGGGAATG 
TTTATCATTC CAACTGCTTA TACTTTGCGA CACGCCCGTG GCTATGGCCG CTCTGCTCTG 
AGAAATTGGA TGTTCCAGAT GTCGAAGGAT GGAATAAATT GGGCGACAAT GCTGACCCAT 
TCGGATGATA AAAGTTTGGC AGAACCCGGA AGCACTTGCA CTTGGCCACT CGAATGCTCT 
GCGGAAGAAC AGCAGGGATG GCGTCACGTG AGAATCCATC AAAATGGACG GAATGCTTCA 
GGGCAGACCC ATTACCTAAG CTTGAGTGGA TTTGAAATCT ACGGAAAGGT TGTCTCGGTT 
TGTGACGATA TGGGAAAAGC TGCGGCCAAG GAGAATGAAG CTCGGCTTAG GAAAGAGCGA 
CGACAGATTC GAGCTCAGTT GAAGTATATC ACTCAAGGTG CACGCGTTGT TCGAGGAGTT 
GATTGGCATT GGGATGATCA AGATGGAAGT CCTCCTGGAG AAGGAACTGT AACTGGTGAG 
ATTCATAACG GTTGGATCGA TGTAAAGTGG GACCATGGTT TAAGAAACTC CTATCGCATG 
GGAGCCGAAG GCAAATATGA TCTGAAGCTG TCAAACAGTG AAAACCTGAC TGCACCTTAC 
GATATGAACA ACTCCGGTGC TGGACTTGTT CCAATTAACG CTGGAAGCGT ATCTTCGGCG 
AAGAAAGTAT ATGATAAGTC GTTGAACGTT CTGACCAGTC GCAAATCGAG CTCTACTCCG 
AGCCTCCCGG AAGCAACGGA GAACAAGTCA TCAGTAGCTT CGACTGAACA AGCCACTTCC 
GTGGATAACC TTGCATGGAA ACAAGCCGTA GAAGTGATTG CCGAAAATGT GCTATCCTGT 
GCTCGATCTG ATTTGGCCAA TACCAGTGGT GGAAGTAGCA GCAATGACTT ATCCGCTCCC 
GGATCCAACA ATAATAATCT GAACAACCAG GAAGTGTCTG TGATTGTCCA CTCGTTGGGA 
GAGCGGGGAA ACATCCCAGA TCTGTCGCAA ATCAATACAA GTACTTCGAC TCTAGTCTCG 
GATTTGGCCA CCATCACGGA GAATCTAACG CTTTCGGATA ACATCAAGAA TAACATCAGC 
ACTGGAAGCA GCACGCAGTT TGTGAGCAAT TTTGGAACCC AACTTGCAGC TTGCTCATCC 
TCGTCTTCGT CCGAAGACAA CAACAAAACC AACAACATCA ACGAAACCAA TAACAAGATC 
AATCTCAACA ACAGCAGCAG CAGTAGTTCC GGCAAAACGG CATACCTGCC AACCAAGCTC 
GACGTGTTGG ACAAAATGAG GGAAGGCGTC GACATGCTTC GGAACAATAC CAACAATTTG 
CTGTCCTCGG AATTGCTCAC CCAGTCAAAT CTACTCTCGT CCGTAAAGAT TGCACTTCCG 
ACGCCACAAC AACCGCAGCA AACTGGAGCG ACAGCTCCCG CAGGACCTTT CTTCGTGGCC 
AGCACAAGCA CCAGCAGCAC GAGCACTCTC CCTTCGGGTG ATAAAATCGT TGGAGATGTC 
AAGTTTAACA ATACCATCAA CAACAATACC GCTTCCGGTG GCGTAACGAA GAAAGTCCTG 
AACGAAGTCA AGCAGCAGGA ACCGGATGAT CGCGACATTG CCAACAATCT GAAGAACAAC 
ACCATTGTCG TCGATTCGGT GGAAGTTGGT GCAGCAGGAA GTTCGAAGGA ATCAACTGCT 
GAGTCACCTT CGGCGGCGGT CACGGTCAAT CCGATGAGTG TGAGTGTCCC GAATCTGACG 
AGCAATGAGA ACAATGCTTC ACAAGAGGTC CAAACTCCAC CAGGGTTGTT GGAAACGTTT 
GCAGCCATAG CTCGAAGGCG TACGTCTGGA AGTTCTGTAT CCCATCATGT GAACCCTAGC 
TCAAACGCTT CAACCGCTTC TTCCGGAACC AGTGGCACAA ACAATACTCA ACCCAACAAT 
CAGCTGAGTA GCCTTGGGTC CAATATTCAA GCAGCGAACT CTAGCTTCTT CCCGAGGGGA 
CAAAACTCCG TTACTAGTCT GGTGAAATTG GCCCTTTCGA GCAACTTCCA CAGTGGATTG 
TTGAGTACCG CACAAAGTTA TCCTAGTCTC TCGAGTTCGA GTTCGAATAA TGCAAATTCG 
GTCGCGGTAA GTGGAGGAAC TAACAACAAT TCGGCGAGCG GCGTCGGTTC CAACAATGGA 
TCGGTTCAAA GCGGAAACGT CCAAGCCGTG TTGAATCCAG CACTGACGAT GAGTCTCACC 
TCAACCAGTA GCGATAGTGA GCAGGTGTCT TTGGAAGATT TCCTTGAACA GTGCCGAGCT 
CCAACATTGC TGGGAGATTT GGACGACGAC GAAGACATGG AGGACGAGAA TGATGACGAT 
GAAAATGAGG ATGAATACGA AGAAGTCGGA AATACTTTGC TCCAAGTGAT GGTTTCTCGT 
AATTTGTTAT CGTTTATGGA TGAGGAGACT TTGGAGAATC GTCTGGCTGC CGCTGGCAAA 
CGCAAATCGT GGGACGACGA ATTCGTGCTT AAACGCCAAT TCTCGGCACT CATTCCTGCA 
TTCGATCCGC GTCCTGGCAG GACCAACGTC AATCAAACTA GTGATCTGGA AATACCTGCT 
CCCGGCAGTT CTACAGACAG TAGTCATCCG AGTAGCAGTC ACTCTGAGCA TGCTCCTTTG 
CCTCAACCGT CCCTTGCACT TCTTCTGCGT GGTCCCAACA TAAACGGAGT AAGTGATGTG 
GAAATTCCAC TGTCGCAGCC CGATTGGACA ATATTCCGAG CGGTCCAAGA ACTGATCCTT 
CAAACCAACA TGACCAAGCA GGATAAGTTC CGCAAAATTT GGCAACCAAC GTACACCATC 
GTCTACAGGG AAGCCTCTTC TTCTGCCGGA CTCGGTTCCT GCCGCGGTGA GGACTTCAGC 
AGCGGAGAAG AAGGACGGGC CACCCCCGTC GTTTCCATGT TTTCCCAACG CAGTGGAGGA 
TCTACCCTTT CGCCTAGCTC TCCGATCCCC GGAACACCAC TCAATCCAAC CGCCACCGCT 
CACTGCACTG TGGACGACGT CTTGCAGCTT CTCGGCCAGT TGAATGCCAT CAATCGAACG 
CTTACATCTT CTCCATCCAA CAACGACAAG AACCTCATCC CGGACATGGA ATCCAACAAT 
CTCAACGCGG ATATATTCTT GAGCAAGAAA ATTACCAACA AACTGCAGCA GCAAATTCAA 
GACCCACTAG TCCTGGCGAG TGGAAGCCTA CCGAAATGGT GCGAAGACTT CAACCAAAGC 
TGTCCGTTCC TGTTCCCGTT CGAGACGCGG CAGCTGTACT TCAACTGTAC GGCTTTCGGC 
GCCTCGAGGA GCATCGTGTG GCTCCAGTCG CAGCGGGATG TGAACCTGGA ACGGCAACGG 
GCTCCCGGGT TGAGCCCACG GCATGCCGAT CAGCACGAGT TCCGCGTTGG GCGGTTGAAG 
CACGAGCGCG TCAAGGTTCC ACGCGGTGAG AATCTACTCG AATGGGCACA ACAGGTTATG 
AAAGTTCACT GTAATCGCAA GTCGGTGTTG GAGGTGGAAT TCGTTGGAGA GGAAGGCACC 
GGATTGGGTC CGACTTTGGA ATTCTACGCC CTGGTTGCAG CTGAACTGCA GCGAAGTGAT 
CTGGGAATGT GGCTGTGCGA CGATGAACCT AAGCTAATCG AAGACGAAAT CGATCTAGGA 
GAGGGAAGCA AACCCATCGG TTATTACGTT CGTCGTTCAA CCGGATTGTT CCCGGCGCCG 
TTGCCACAGG AATCTGAAAT TTGTGACTTT GTGTCAAACT ACTTCTGGTT CTTGGGAGTG 
TTTCTGGCGA AGGTACTGCA GGACGGTCGT CTAGTCGATC TGCCACTGTC GAACAGCTTC 
TTACAACTGT TATGTAATAA CAAATCTCTC GCTAGAGGTT CACTAAGCGA ACTTTCTTCC 
AAGTCTGCCT TGCACGATGA TGTGATGATT TCTTCTTTAA TGTCCGAGGA AAGCGATCGT 
GATCTGGTGG ATTCATATCA GTCAAAATTG GCTAACGATG GCTGTTGGTA TGATGGAATC 
CTGTCCCAAG AAAATCTCCA GGAAATCGAT CCGATTCGGT ACGAGTTCTT GAAAGAACTG 
CAAGAGTTGG TGCAGCAGAA ACAAAATATC GAACAAAATG ATGATCTCAC CTCCGAAGAG 
AAGCTACTGC AAATTAGCGA GCTTAAATTC AACACCAAAA CTGGCAGTGT AGCTTTGGAG 
GACCTCGCTC TAACGTTCAC CTATCTACCA AGTTCGAAGA ACTACGGCTA CCAATCGGCA 
GATCTGATTC CAAACGGTTC CAACATTGAT GTAACGATCA ACAACGTCGA AGAGTACTGC 
AATTTGACGA TCAACTTCTG CCTGCAAGAA GGCATTGCTA AGCAACTTAC TGCGTTCCAT 
CGAGGTTTCT GTGAAGTGTT CCCGCTGAAC AAATTGGCCG CTTTCACATC GGAAGAAATC 
CGCAAGATGC TTTGTGGCGA GCAGAACCCA GAGTGGACAC GGGAGGACAT TATGACCTAC 
ACGGAACCGA AACTTGGATA CTCCAAGGAA AGCCCTGGAT TCCTGCGTTT CGTGAACGTG 
TTGATGGGAA TGAATGCCAG CGAAAGGAAA GCATTTTTGC AGTTCACCAC CGGCTGCAGC 
AGTTTACCAC CCGGTGGGCT GGCCAATTTG CACCCGCGGC TGACAGTGGT GCGGAAGGTA 
GATGCCGGCG AAGGTTCCTA CCCTTCGGTC AACACCTGTG TCCACTACCT CAAATTGCCG 
GACTATCCGA ATGAGGAAAT CTTGCGGGAA CGATTGCTGA CGGCCACAAA AGAAAAGGGA 
TTCCATCTTA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001652833.1
CDS	1..8535
Translation	MTSETRAACHRKTNRPTVSLSSRSDDVRMGDVDPETLLEWLSMGQGDERDMQLIALEQLCMLLLMSDNVDRCFESCPPRTFLPALCKIFLDELAPENVLEVTARAITYYLDVSSECTRRIVAIDGAIKAICNRLVVADLESRTSRDLAEQCIKVLELICTREAGAVFEGGGLNCVLSFIRDNGSQIHKDTLHSAMAVVSRLCTKVEPQGANVQTCVESLSTLLQHEDPLVADGALKCFASVADRFTRKGVDPAPLAEYGLVTELLNRLSNAAGPQTSALTAGASSNATPAAQVNSQETTGTVQLSSSAPKTQGAAEAGRSSQSIATTISLLSTLCRGSPSITHDLLRSNLPDAMERAFKGDERCVLDCMRLADLILLLLFEGRQALGRVGGSSGQLAPRVRRADSSTERTHRQLIDCIRSKDTEALIEAIESGGIDVNCMDDVGQTLLNWASAFGTLEMVEFLCDKGADVNKGQRSSSLHYAACFGRPGIAKVLLKHGANPDLRDEDGKTPLDKARERPDEGHREVASILQSPGEWMTAATRSDLKTDPEDGESEPRGDPEMAPVYLKFFLPIFCKTFQSTMLASVRRSSLGLIKKMIQYVQPEMLSRLCSSEGLQSHEHSLGTLLVEVVASVLDNEDDEDGHLVVLTIIEELMSKTQDDFLDHFARLGVFSKVQALMGENGFSGGEGDNNDVIKSQDELKPSATGAQDASVVSTTGSSSSGTATTNTVEDAKEILPGKAYHWRDWSICRGRDCLYVWSDSAALELSNGSNGWFRFILDGKLATMYSSGSPENGSDSSENRGEFLEKLQRARGAVRQGTPSQPILSSPSLSRIVVGNWVLQSQKEHQLHINNSEGHQVTILQDELPGFIFESNRGTKHTFTAETTLGPDFAAGWINTKKKKMRSKAEAQKYQVKNIARDLYNRYFKAAQAVPRGAVAKLCTIVRQIEAALEEQCAPKASSLLQVRIQPDASKATNSTWQEKLYNALHDLVQLLNDDGVISAYEMHSSGLVQSLVAVLSRNYWELEMNRSKANKYQKQRISIFKKCMYGDTKNGKNTASILVQKLVSVLESIEKLPVHMYDSPGGSYGLQILTKRLSFRLERAACEQTLFDRTGRNLKMEPLATVGQLNKYLLKMVAKQWYDMDRSSFLYLKKLKEAKPGSVHFKHQHDFDENGLVYYIGTNGKTTEWVNPAQYGLVTVTSSEGKQLPYGKLEDILSRDSVSVNCHTKDNKKSWFAIDLGMFIIPTAYTLRHARGYGRSALRNWMFQMSKDGINWATMLTHSDDKSLAEPGSTCTWPLECSAEEQQGWRHVRIHQNGRNASGQTHYLSLSGFEIYGKVVSVCDDMGKAAAKENEARLRKERRQIRAQLKYITQGARVVRGVDWHWDDQDGSPPGEGTVTGEIHNGWIDVKWDHGLRNSYRMGAEGKYDLKLSNSENLTAPYDMNNSGAGLVPINAGSVSSAKKVYDKSLNVLTSRKSSSTPSLPEATENKSSVASTEQATSVDNLAWKQAVEVIAENVLSCARSDLANTSGGSSSNDLSAPGSNNNNLNNQEVSVIVHSLGERGNIPDLSQINTSTSTLVSDLATITENLTLSDNIKNNISTGSSTQFVSNFGTQLAACSSSSSSEDNNKTNNINETNNKINLNNSSSSSSGKTAYLPTKLDVLDKMREGVDMLRNNTNNLLSSELLTQSNLLSSVKIALPTPQQPQQTGATAPAGPFFVASTSTSSTSTLPSGDKIVGDVKFNNTINNNTASGGVTKKVLNEVKQQEPDDRDIANNLKNNTIVVDSVEVGAAGSSKESTAESPSAAVTVNPMSVSVPNLTSNENNASQEVQTPPGLLETFAAIARRRTSGSSVSHHVNPSSNASTASSGTSGTNNTQPNNQLSSLGSNIQAANSSFFPRGQNSVTSLVKLALSSNFHSGLLSTAQSYPSLSSSSSNNANSVAVSGGTNNNSASGVGSNNGSVQSGNVQAVLNPALTMSLTSTSSDSEQVSLEDFLEQCRAPTLLGDLDDDEDMEDENDDDENEDEYEEVGNTLLQVMVSRNLLSFMDEETLENRLAAAGKRKSWDDEFVLKRQFSALIPAFDPRPGRTNVNQTSDLEIPAPGSSTDSSHPSSSHSEHAPLPQPSLALLLRGPNINGVSDVEIPLSQPDWTIFRAVQELILQTNMTKQDKFRKIWQPTYTIVYREASSSAGLGSCRGEDFSSGEEGRATPVVSMFSQRSGGSTLSPSSPIPGTPLNPTATAHCTVDDVLQLLGQLNAINRTLTSSPSNNDKNLIPDMESNNLNADIFLSKKITNKLQQQIQDPLVLASGSLPKWCEDFNQSCPFLFPFETRQLYFNCTAFGASRSIVWLQSQRDVNLERQRAPGLSPRHADQHEFRVGRLKHERVKVPRGENLLEWAQQVMKVHCNRKSVLEVEFVGEEGTGLGPTLEFYALVAAELQRSDLGMWLCDDEPKLIEDEIDLGEGSKPIGYYVRRSTGLFPAPLPQESEICDFVSNYFWFLGVFLAKVLQDGRLVDLPLSNSFLQLLCNNKSLARGSLSELSSKSALHDDVMISSLMSEESDRDLVDSYQSKLANDGCWYDGILSQENLQEIDPIRYEFLKELQELVQQKQNIEQNDDLTSEEKLLQISELKFNTKTGSVALEDLALTFTYLPSSKNYGYQSADLIPNGSNIDVTINNVEEYCNLTINFCLQEGIAKQLTAFHRGFCEVFPLNKLAAFTSEEIRKMLCGEQNPEWTREDIMTYTEPKLGYSKESPGFLRFVNVLMGMNASERKAFLQFTTGCSSLPPGGLANLHPRLTVVRKVDAGEGSYPSVNTCVHYLKLPDYPNEEILRERLLTATKEKGFHLN

Target ORF information:

RefSeq Version	XM_001652783.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL007705-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001652783.1

ORF Insert Sequence:

ATGACATCTG AAACGAGAGC AGCTTGCCAC AGGAAAACAA ACCGTCCAAC AGTGTCGCTC 
TCGTCTCGTT CAGACGACGT TCGAATGGGT GACGTTGATC CAGAAACGCT GCTAGAATGG 
CTGTCGATGG GGCAGGGAGA CGAACGGGAT ATGCAGTTGA TTGCCCTGGA GCAGCTATGT 
ATGCTTCTGC TGATGTCCGA CAACGTTGAT CGTTGTTTCG AAAGTTGTCC CCCGCGAACG 
TTCCTCCCAG CGCTGTGTAA AATATTTTTA GATGAACTAG CGCCAGAGAA TGTACTGGAA 
GTGACAGCAC GTGCCATCAC CTACTATCTA GACGTATCAT CCGAGTGCAC ACGTCGCATT 
GTAGCGATCG ATGGTGCGAT CAAAGCTATC TGCAACCGGT TGGTGGTGGC CGATCTCGAA 
AGCAGAACTA GTCGTGATCT TGCGGAGCAG TGCATCAAAG TACTTGAGCT CATATGCACT 
CGAGAAGCAG GGGCCGTGTT TGAAGGAGGT GGACTTAATT GTGTGCTTTC ATTCATTCGG 
GACAATGGGT CTCAGATCCA CAAAGACACA CTCCACTCGG CAATGGCCGT GGTGTCCAGG 
CTATGTACCA AGGTGGAGCC ACAAGGTGCT AATGTACAAA CTTGTGTAGA AAGTCTAAGT 
ACTCTGCTAC AGCACGAAGA TCCGCTCGTA GCAGACGGAG CATTGAAATG CTTTGCTTCG 
GTGGCAGATC GATTTACTAG AAAAGGAGTT GATCCTGCCC CACTTGCAGA GTATGGACTA 
GTAACTGAGT TGTTAAATCG ACTAAGCAAC GCTGCAGGAC CACAAACATC AGCCTTGACA 
GCCGGAGCTA GTTCAAATGC GACGCCCGCT GCACAGGTCA ACAGTCAAGA GACCACAGGA 
ACAGTGCAGC TTTCATCGTC AGCACCAAAA ACACAGGGAG CAGCCGAAGC AGGGCGATCG 
AGTCAATCGA TCGCCACAAC TATATCACTG TTATCTACCT TGTGTCGAGG GTCACCATCG 
ATCACGCATG ATCTCCTCCG ATCCAATCTA CCAGATGCTA TGGAACGAGC CTTCAAAGGT 
GATGAACGAT GTGTTCTTGA CTGTATGAGG CTTGCCGATC TCATCCTGCT GCTGTTGTTC 
GAAGGTCGAC AAGCTCTTGG CAGGGTGGGT GGATCTAGCG GTCAGTTAGC TCCTCGTGTG 
CGAAGAGCAG ACTCTAGTAC TGAGCGAACA CATCGACAGT TGATCGATTG CATACGTAGT 
AAGGACACCG AAGCCCTAAT CGAAGCTATC GAATCGGGTG GAATCGATGT CAACTGCATG 
GACGATGTCG GTCAAACATT ACTTAACTGG GCTTCGGCCT TTGGTACATT AGAAATGGTA 
GAGTTTTTAT GTGATAAAGG TGCTGACGTC AACAAAGGCC AGCGAAGTTC CTCTCTGCAT 
TATGCGGCCT GTTTCGGTCG ACCCGGCATA GCAAAAGTAC TGCTCAAACA CGGTGCCAAT 
CCTGACCTAC GGGACGAAGA CGGCAAAACA CCACTGGATA AGGCTCGCGA ACGGCCGGAT 
GAAGGCCACC GTGAAGTTGC TTCCATTCTT CAGTCGCCTG GCGAATGGAT GACCGCGGCA 
ACACGATCGG ACTTGAAAAC CGACCCCGAA GACGGGGAAA GCGAGCCCCG CGGAGATCCG 
GAGATGGCAC CCGTCTATCT CAAGTTCTTT CTACCAATAT TCTGCAAGAC GTTCCAAAGC 
ACAATGCTAG CCAGCGTTCG ACGGTCTAGT TTAGGTTTGA TCAAGAAGAT GATTCAATAT 
GTCCAACCAG AAATGCTGTC GAGGCTTTGT TCCTCGGAGG GCCTCCAAAG TCATGAGCAC 
AGCCTAGGAA CTTTACTAGT CGAAGTCGTC GCCAGTGTTT TGGACAACGA GGACGACGAA 
GATGGCCATC TGGTGGTGCT TACCATTATC GAAGAGCTCA TGTCCAAGAC GCAAGACGAT 
TTCCTCGATC ACTTTGCCCG GCTCGGAGTG TTCTCCAAAG TGCAGGCCCT GATGGGAGAA 
AACGGCTTCA GCGGGGGCGA AGGTGATAAC AACGATGTGA TCAAATCCCA AGACGAGCTC 
AAACCGTCTG CGACCGGAGC ACAAGATGCC TCGGTTGTCT CCACTACTGG AAGCTCCTCC 
TCTGGAACTG CCACTACTAA TACGGTGGAA GATGCCAAAG AAATCCTTCC CGGGAAGGCG 
TACCACTGGC GAGACTGGAG TATTTGTCGA GGTCGCGACT GCTTGTACGT GTGGTCGGAT 
TCAGCCGCCT TGGAGCTTTC GAACGGATCC AATGGGTGGT TCCGGTTCAT CCTGGACGGG 
AAATTGGCCA CTATGTACTC CAGTGGTAGT CCAGAGAATG GCAGTGATAG CTCAGAAAAC 
CGTGGTGAAT TCCTAGAAAA ATTACAACGA GCTCGCGGAG CGGTACGGCA GGGAACTCCC 
TCCCAGCCGA TTCTTTCGTC GCCGAGCCTT TCGCGAATCG TGGTCGGCAA TTGGGTGCTG 
CAGAGCCAAA AAGAGCATCA GCTACATATC AACAACTCCG AAGGTCACCA GGTGACGATC 
CTGCAGGACG AACTGCCCGG ATTCATCTTC GAGAGCAATC GCGGCACGAA GCATACTTTC 
ACGGCGGAAA CCACTCTGGG ACCGGACTTC GCCGCCGGAT GGATCAACAC CAAGAAGAAG 
AAGATGCGTT CGAAGGCGGA GGCTCAGAAA TATCAGGTTA AAAACATCGC CCGAGATCTG 
TACAACCGGT ATTTCAAAGC AGCTCAAGCC GTCCCGCGGG GCGCCGTGGC AAAACTGTGC 
ACCATCGTGC GTCAAATCGA GGCAGCCCTG GAGGAACAAT GTGCCCCTAA GGCGTCCAGT 
TTGCTGCAGG TTCGGATTCA ACCTGACGCT TCTAAAGCAA CGAACAGTAC ATGGCAGGAG 
AAGCTGTACA ACGCTCTGCA CGATCTGGTG CAACTGTTGA ACGACGATGG AGTCATCAGT 
GCTTACGAGA TGCACAGTTC CGGACTTGTA CAATCCTTGG TGGCCGTCCT GTCCCGGAAC 
TACTGGGAAC TCGAAATGAA CCGGAGCAAG GCCAACAAGT ACCAGAAACA GCGGATATCC 
ATTTTCAAAA AGTGCATGTA TGGCGATACC AAAAATGGCA AAAACACGGC CAGCATTCTG 
GTGCAGAAGT TGGTATCTGT ATTGGAGAGC ATTGAGAAGC TTCCGGTGCA CATGTATGAC 
TCCCCAGGGG GTAGCTATGG GTTGCAGATT CTCACGAAGC GATTGAGCTT CCGACTTGAG 
CGGGCAGCTT GTGAGCAAAC TCTCTTCGAT AGGACCGGTA GAAACCTGAA AATGGAACCA 
TTGGCAACCG TTGGCCAGCT GAACAAGTAT CTTCTGAAAA TGGTAGCAAA GCAGTGGTAC 
GATATGGATC GATCGTCCTT CTTGTACTTG AAAAAGCTGA AAGAGGCAAA ACCAGGAAGT 
GTACACTTCA AGCACCAGCA CGATTTCGAC GAGAATGGTC TTGTGTACTA TATCGGGACG 
AACGGAAAAA CCACGGAATG GGTCAATCCT GCTCAGTACG GATTGGTTAC TGTCACAAGT 
AGCGAAGGCA AACAGCTCCC GTACGGTAAG CTGGAAGATA TCCTGTCGAG AGATAGCGTT 
AGCGTGAATT GTCACACCAA GGATAACAAG AAATCGTGGT TCGCCATCGA TTTGGGAATG 
TTTATCATTC CAACTGCTTA TACTTTGCGA CACGCCCGTG GCTATGGCCG CTCTGCTCTG 
AGAAATTGGA TGTTCCAGAT GTCGAAGGAT GGAATAAATT GGGCGACAAT GCTGACCCAT 
TCGGATGATA AAAGTTTGGC AGAACCCGGA AGCACTTGCA CTTGGCCACT CGAATGCTCT 
GCGGAAGAAC AGCAGGGATG GCGTCACGTG AGAATCCATC AAAATGGACG GAATGCTTCA 
GGGCAGACCC ATTACCTAAG CTTGAGTGGA TTTGAAATCT ACGGAAAGGT TGTCTCGGTT 
TGTGACGATA TGGGAAAAGC TGCGGCCAAG GAGAATGAAG CTCGGCTTAG GAAAGAGCGA 
CGACAGATTC GAGCTCAGTT GAAGTATATC ACTCAAGGTG CACGCGTTGT TCGAGGAGTT 
GATTGGCATT GGGATGATCA AGATGGAAGT CCTCCTGGAG AAGGAACTGT AACTGGTGAG 
ATTCATAACG GTTGGATCGA TGTAAAGTGG GACCATGGTT TAAGAAACTC CTATCGCATG 
GGAGCCGAAG GCAAATATGA TCTGAAGCTG TCAAACAGTG AAAACCTGAC TGCACCTTAC 
GATATGAACA ACTCCGGTGC TGGACTTGTT CCAATTAACG CTGGAAGCGT ATCTTCGGCG 
AAGAAAGTAT ATGATAAGTC GTTGAACGTT CTGACCAGTC GCAAATCGAG CTCTACTCCG 
AGCCTCCCGG AAGCAACGGA GAACAAGTCA TCAGTAGCTT CGACTGAACA AGCCACTTCC 
GTGGATAACC TTGCATGGAA ACAAGCCGTA GAAGTGATTG CCGAAAATGT GCTATCCTGT 
GCTCGATCTG ATTTGGCCAA TACCAGTGGT GGAAGTAGCA GCAATGACTT ATCCGCTCCC 
GGATCCAACA ATAATAATCT GAACAACCAG GAAGTGTCTG TGATTGTCCA CTCGTTGGGA 
GAGCGGGGAA ACATCCCAGA TCTGTCGCAA ATCAATACAA GTACTTCGAC TCTAGTCTCG 
GATTTGGCCA CCATCACGGA GAATCTAACG CTTTCGGATA ACATCAAGAA TAACATCAGC 
ACTGGAAGCA GCACGCAGTT TGTGAGCAAT TTTGGAACCC AACTTGCAGC TTGCTCATCC 
TCGTCTTCGT CCGAAGACAA CAACAAAACC AACAACATCA ACGAAACCAA TAACAAGATC 
AATCTCAACA ACAGCAGCAG CAGTAGTTCC GGCAAAACGG CATACCTGCC AACCAAGCTC 
GACGTGTTGG ACAAAATGAG GGAAGGCGTC GACATGCTTC GGAACAATAC CAACAATTTG 
CTGTCCTCGG AATTGCTCAC CCAGTCAAAT CTACTCTCGT CCGTAAAGAT TGCACTTCCG 
ACGCCACAAC AACCGCAGCA AACTGGAGCG ACAGCTCCCG CAGGACCTTT CTTCGTGGCC 
AGCACAAGCA CCAGCAGCAC GAGCACTCTC CCTTCGGGTG ATAAAATCGT TGGAGATGTC 
AAGTTTAACA ATACCATCAA CAACAATACC GCTTCCGGTG GCGTAACGAA GAAAGTCCTG 
AACGAAGTCA AGCAGCAGGA ACCGGATGAT CGCGACATTG CCAACAATCT GAAGAACAAC 
ACCATTGTCG TCGATTCGGT GGAAGTTGGT GCAGCAGGAA GTTCGAAGGA ATCAACTGCT 
GAGTCACCTT CGGCGGCGGT CACGGTCAAT CCGATGAGTG TGAGTGTCCC GAATCTGACG 
AGCAATGAGA ACAATGCTTC ACAAGAGGTC CAAACTCCAC CAGGGTTGTT GGAAACGTTT 
GCAGCCATAG CTCGAAGGCG TACGTCTGGA AGTTCTGTAT CCCATCATGT GAACCCTAGC 
TCAAACGCTT CAACCGCTTC TTCCGGAACC AGTGGCACAA ACAATACTCA ACCCAACAAT 
CAGCTGAGTA GCCTTGGGTC CAATATTCAA GCAGCGAACT CTAGCTTCTT CCCGAGGGGA 
CAAAACTCCG TTACTAGTCT GGTGAAATTG GCCCTTTCGA GCAACTTCCA CAGTGGATTG 
TTGAGTACCG CACAAAGTTA TCCTAGTCTC TCGAGTTCGA GTTCGAATAA TGCAAATTCG 
GTCGCGGTAA GTGGAGGAAC TAACAACAAT TCGGCGAGCG GCGTCGGTTC CAACAATGGA 
TCGGTTCAAA GCGGAAACGT CCAAGCCGTG TTGAATCCAG CACTGACGAT GAGTCTCACC 
TCAACCAGTA GCGATAGTGA GCAGGTGTCT TTGGAAGATT TCCTTGAACA GTGCCGAGCT 
CCAACATTGC TGGGAGATTT GGACGACGAC GAAGACATGG AGGACGAGAA TGATGACGAT 
GAAAATGAGG ATGAATACGA AGAAGTCGGA AATACTTTGC TCCAAGTGAT GGTTTCTCGT 
AATTTGTTAT CGTTTATGGA TGAGGAGACT TTGGAGAATC GTCTGGCTGC CGCTGGCAAA 
CGCAAATCGT GGGACGACGA ATTCGTGCTT AAACGCCAAT TCTCGGCACT CATTCCTGCA 
TTCGATCCGC GTCCTGGCAG GACCAACGTC AATCAAACTA GTGATCTGGA AATACCTGCT 
CCCGGCAGTT CTACAGACAG TAGTCATCCG AGTAGCAGTC ACTCTGAGCA TGCTCCTTTG 
CCTCAACCGT CCCTTGCACT TCTTCTGCGT GGTCCCAACA TAAACGGAGT AAGTGATGTG 
GAAATTCCAC TGTCGCAGCC CGATTGGACA ATATTCCGAG CGGTCCAAGA ACTGATCCTT 
CAAACCAACA TGACCAAGCA GGATAAGTTC CGCAAAATTT GGCAACCAAC GTACACCATC 
GTCTACAGGG AAGCCTCTTC TTCTGCCGGA CTCGGTTCCT GCCGCGGTGA GGACTTCAGC 
AGCGGAGAAG AAGGACGGGC CACCCCCGTC GTTTCCATGT TTTCCCAACG CAGTGGAGGA 
TCTACCCTTT CGCCTAGCTC TCCGATCCCC GGAACACCAC TCAATCCAAC CGCCACCGCT 
CACTGCACTG TGGACGACGT CTTGCAGCTT CTCGGCCAGT TGAATGCCAT CAATCGAACG 
CTTACATCTT CTCCATCCAA CAACGACAAG AACCTCATCC CGGACATGGA ATCCAACAAT 
CTCAACGCGG ATATATTCTT GAGCAAGAAA ATTACCAACA AACTGCAGCA GCAAATTCAA 
GACCCACTAG TCCTGGCGAG TGGAAGCCTA CCGAAATGGT GCGAAGACTT CAACCAAAGC 
TGTCCGTTCC TGTTCCCGTT CGAGACGCGG CAGCTGTACT TCAACTGTAC GGCTTTCGGC 
GCCTCGAGGA GCATCGTGTG GCTCCAGTCG CAGCGGGATG TGAACCTGGA ACGGCAACGG 
GCTCCCGGGT TGAGCCCACG GCATGCCGAT CAGCACGAGT TCCGCGTTGG GCGGTTGAAG 
CACGAGCGCG TCAAGGTTCC ACGCGGTGAG AATCTACTCG AATGGGCACA ACAGGTTATG 
AAAGTTCACT GTAATCGCAA GTCGGTGTTG GAGGTGGAAT TCGTTGGAGA GGAAGGCACC 
GGATTGGGTC CGACTTTGGA ATTCTACGCC CTGGTTGCAG CTGAACTGCA GCGAAGTGAT 
CTGGGAATGT GGCTGTGCGA CGATGAACCT AAGCTAATCG AAGACGAAAT CGATCTAGGA 
GAGGGAAGCA AACCCATCGG TTATTACGTT CGTCGTTCAA CCGGATTGTT CCCGGCGCCG 
TTGCCACAGG AATCTGAAAT TTGTGACTTT GTGTCAAACT ACTTCTGGTT CTTGGGAGTG 
TTTCTGGCGA AGGTACTGCA GGACGGTCGT CTAGTCGATC TGCCACTGTC GAACAGCTTC 
TTACAACTGT TATGTAATAA CAAATCTCTC GCTAGAGGTT CACTAAGCGA ACTTTCTTCC 
AAGTCTGCCT TGCACGATGA TGTGATGATT TCTTCTTTAA TGTCCGAGGA AAGCGATCGT 
GATCTGGTGG ATTCATATCA GTCAAAATTG GCTAACGATG GCTGTTGGTA TGATGGAATC 
CTGTCCCAAG AAAATCTCCA GGAAATCGAT CCGATTCGGT ACGAGTTCTT GAAAGAACTG 
CAAGAGTTGG TGCAGCAGAA ACAAAATATC GAACAAAATG ATGATCTCAC CTCCGAAGAG 
AAGCTACTGC AAATTAGCGA GCTTAAATTC AACACCAAAA CTGGCAGTGT AGCTTTGGAG 
GACCTCGCTC TAACGTTCAC CTATCTACCA AGTTCGAAGA ACTACGGCTA CCAATCGGCA 
GATCTGATTC CAAACGGTTC CAACATTGAT GTAACGATCA ACAACGTCGA AGAGTACTGC 
AATTTGACGA TCAACTTCTG CCTGCAAGAA GGCATTGCTA AGCAACTTAC TGCGTTCCAT 
CGAGGTTTCT GTGAAGTGTT CCCGCTGAAC AAATTGGCCG CTTTCACATC GGAAGAAATC 
CGCAAGATGC TTTGTGGCGA GCAGAACCCA GAGTGGACAC GGGAGGACAT TATGACCTAC 
ACGGAACCGA AACTTGGATA CTCCAAGGAA AGCCCTGGAT TCCTGCGTTT CGTGAACGTG 
TTGATGGGAA TGAATGCCAG CGAAAGGAAA GCATTTTTGC AGTTCACCAC CGGCTGCAGC 
AGTTTACCAC CCGGTGGGCT GGCCAATTTG CACCCGCGGC TGACAGTGGT GCGGAAGGTA 
GATGCCGGCG AAGGTTCCTA CCCTTCGGTC AACACCTGTG TCCACTACCT CAAATTGCCG 
GACTATCCGA ATGAGGAAAT CTTGCGGGAA CGATTGCTGA CGGCCACAAA AGAAAAGGGA 
TTCCATCTTA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL007705 ( XM_021848323.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021704015.1 Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X2, mRNA.

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CloneID	OAe124645
Clone ID Related Accession (Same CDS sequence)	XM_021848323.1 , XM_021848322.1
Accession Version	XM_021848323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGATA AGCTACAGCT GGCCGTCACA GCCATCAGCA 
CCATCTCTAC TGCCCTTAAC CGTTTCCTCC TCTTCTACCA ATACTACTAG TAGCAATAGT 
ATTCTAGCCA AATATAGTTG CGCTGACCGA GTAAGCAAGT ATGTCCAGAA CCTGCGACTG 
CAACAAAACC AGCAGCAGCC ACCAGAGCGG CGTCGTAACT CTAGTTCTTA CATAATTCCA 
CCGCCGCCGC CAATGCTGCC GTTGACCTCC TCCCAGGACG ACGAAGATGG CCATCTGGTG 
GTGCTTACCA TTATCGAAGA GCTCATGTCC AAGACGCAAG ACGATTTCCT CGATCACTTT 
GCCCGGCTCG GAGTGTTCTC CAAAGTTCAG GCCCTGATGG GAGAAAACGG CTTCAGCGGG 
GGCGAAGGTG ATAACAACGA TGTGATCAAA TCCCAAGACG AGCTCAAACC GTCTGCGACC 
GGAGCACAAG ATGCTTCGGT TGTCTCCACT ACTGGAAGCT CCTCCTCTGG AACTGCCACT 
ACTAATACGG TGGAAGATGC CAAAGAAATC CTTCCCGGGA AGGCGTACCA CTGGCGAGAC 
TGGAGTATTT GTCGAGGTCG CGACTGCTTG TACGTATGGT CGGATTCAGC CGCCTTGGAG 
CTTTCGAACG GATCCAATGG GTGGTTCCGG TTCATCCTAG ACGGGAAATT GGCCACTATG 
TACTCCAGTG GTAGTCCAGA GAATGGCAGT GATAGCTCAG GCAAGGGAAG GACAGCGGAT 
TCCTTAGCCG GCGAAGAAAA CCGTGGTGAA TTCCTAGAAA AATTACAACG AGCTCGCGGA 
GCGGTACGGC AGGGAACTCC CTCCCAGCCG ATTCTTTCGT CGCCGAGCCT TTCGCGAATC 
GTGGTCGGCA ATTGGGTGCT GCAGAGCCAA AAAGAGCATC AGCTACATAT CAACAACTCC 
GAAGGTCACC AGGTGACGAT CCTGCAGGAC GAACTGCCCG GATTCATCTT CGAGAGCAAT 
CGCGGCACGA AGCATACTTT CACGGCGGAA ACCACTCTAG GACCGGACTT CGCCGCCGGA 
TGGATCAACA CCAAGAAGAA GAAGATGCGT TCGAAAGCGG AGGCTCAGAA ATATCAGGTT 
AAAAACATCG CCCGAGATCT GTACAACCGG TACTTCAAAG CAGCTCAAGC CGTCCCGCGG 
GGCGCCGTGG CAAAACTGTG CACCATCGTG CGTCAAATCG AGGCAGCCCT GGAGGAACAA 
TGTGCCCCTA AGGCGTCCAG TTTGCTGCAG GTTCGGATTC AACCTGACGC TTCTAAAGCA 
ACGAACAGCA CATGGCAGGA GAAGCTGTAC AACGCTCTGC ACGATCTTGT GCAACTGTTG 
AACGACGATG GAGTCATCAG TGCTTACGAG ATGCACAGTT CCGGACTTGT ACAATCCTTG 
GTGGCCGTGC TGTCCCGGAA CTACTGGGAA CTGGAGATGA ACCGGAGCAA GGCCAACAAG 
TACCAGAAGC AACGGATATC CATTTTCAAG AAGTGCATGT ATGGCGATAC CAAAAATGGC 
AAAAACACGG CCAGCATTCT GGTGCAGAAG TTGGTATCTG TATTGGAGAG CATTGAGAAG 
CTTCCGGTGC ACATGTATGA CTCCCCAGGG GGTAGCTATG GGTTGCAGAT TCTCACAAAG 
CGACTGAGCT TCCGACTTGA GCGGGCAGCT TGTGAGCAAA CTCTCTTCGA TAGGACCGGT 
AGAAACCTGA AAATGGAACC ATTGGCAACC GTTGGCCAGC TGAACAAGTA TCTTCTGAAA 
ATGGTAGCAA AGCAGTGGTA CGATATGGAT CGATCGTCCT TCTTGTACTT GAAAAAGCTG 
AAAGAGGCAA AACCAGGAAG TGTACACTTC AAGCACCAGC ACGATTTCGA CGAGAATGGT 
CTCGTGTACT ATATCGGGAC GAACGGAAAA ACCACGGAAT GGGTCAATCC TGCTCAGTAC 
GGATTGGTTA CTGTCACAAG TAGCGAAGGC AAACAGCTCC CGTACGGTAA GCTGGAAGAT 
ATCCTGTCGA GAGATAGCGT TAGCGTGAAT TGTCACACCA AGGATAACAA GAAATCGTGG 
TTCGCCATCG ATTTGGGAAT GTTTATCATT CCAACTGCTT ATACTTTGCG ACACGCCCGT 
GGCTATGGCC GCTCTGCTCT GAGAAATTGG ATGTTCCAGA TGTCAAAGGA TGGAATAAAT 
TGGGCGACAA TGTTGACCCA TTCGGATGAT AAAAGTTTGG CAGAACCCGG AAGCACTTGC 
ACTTGGCCAC TCGAATGCTC TGCCGAAGAA CAGCAGGGAT GGCGTCACGT GAGAATCCAT 
CAAAATGGAC GGAATGCTTC AGGGCAGACC CATTACCTTA GCTTGAGTGG ATTTGAAATC 
TACGGAAAGG TTGTCTCGGT TTGTGATGAT ATGGGAAAAG CTGCGGCCAA GGAGAATGAA 
GCTCGGCTTA GGAAAGAGCG ACGACAGATT CGAGCTCAGT TGAAGTATAT CACTCAAGGT 
GCACGCGTTG TTCGAGGAGT TGATTGGCAT TGGGATGATC AAGATGGAAG TCCTCCTGGA 
GAAGGAACCG TAACTGGTGA GATTCATAAC GGTTGGATCG ATGTAAAGTG GGACCATGGT 
TTAAGAAACT CCTATCGCAT GGGAGCCGAA GGCAAATATG ATCTGAAGCT GTCAAACAGT 
GAAAACCTGA CTGCACCTTA CGATATGAAC AACTCCGGTG CTGGACTTGT TCCAATTAAC 
GCAGGAAGCG TGTCTTCGGC GAAGAAAGTA TATGATAAGT CGTTGAACGT TCTGACCAGT 
CGCAAATCGA GCTCTACTCC GAGCCTCCCG GAAGCAACGG AGAACAAGTC ATCAGTAGCT 
TCGACTGAAC AAGCCACTTC CGTGGATAAC CTTGCATGGA AACAAGCCGT AGAAGTGATT 
GCCGAAAATG TGCTATCCTG TGCTCGATCT GATTTGGCCA ATACCAGTGG TGGAAGTAGC 
AGCAATGACT TATCCGCTCC CGGATCCAAC AATAATAATC TGAACAACCA GGAAGTGTCT 
GTGATTGTCC ACTCGTTGGG AGAGCGGGGA AACATCCCAG ATCTGTCGCA AATCAATACA 
AGTACTTCGA CTCTAGTCTC GGATTTGGCC ACCATCACGG AGAATCTAAC ACTCTCGGAT 
AACATCAAGA ACAACATCAG CATCGGAAGT AGCACCCAGT TTGTGAGCAA CTTTGGAACC 
CAACTTGCAG CTTGCTCATC CTCGTCTTCG TCCGAAGACA ACAACAAAAC CAACAACATC 
AATGAAACCA ATAACAAAAT CAATCTCAAC AACAGCAGCA GCAGTAGTTC CGGTAAAACG 
GCATACCTGC CAACCAAGCT CGACGTGTTG GACAAAATGA GGGAAGGCGT CGACATGCTT 
CGGAACAATA CCAACAATTT GCTGTCCTCG GAATTGCTCA CCCAGTCAAA TCTACTCTCG 
TCCGTAAAGA TTGCACTTCC GACGCCACAA CAACCGCAGC AAACTGGAGC GACAGCTCCC 
TCAGGACCTT TCTTCGTGGC CAGCACAAGC ACTAGCAGCA CGAGCACTCT CCCTTCGGGT 
GATAAAATCG TCGGAGATGT CAAGTTTAAC AATACCATCA ACAATAATAC CGCTTCCGGT 
GGCGTAACGA AGAAAGTCCT GAACGAAGTC AAACAGCAGG AACCGGACGA TCGCGACATT 
GCCAACAATT TGAAGAACAA CACCATTGTC GTCGATTCGG TGGAAGTTGG TGCAGCAGGA 
AGTTCGAAGG AATCAACTGC TGAGTCACCT TCGGCGGCGG TCACGGTCAA TCCGATGAGT 
GTGAGTGTCC CAAATCTGAC GAGCAATGAT AACAATGCTT CACAGGAGGT ACAAACTCCA 
CCAGGGTTGT TGGAAACGTT TGCGGCCATA GCTCGAAGGC GTACTTCTGG AAGCTCTGTA 
TCCCATCATG TGAACCCTAG CTCAAACGCT TCAACCGCTT CTTCCGGAAC CAGTGGCACA 
AACAATGCTC AACCCAACAA TCAGCTGAGT AGCCTTGGGT CCAATATTCA AGCAGCGAAC 
TCTAGCTTCT TCCCGAGGGG GCAAAACTCC GTTACTAGTC TGGTGAAATT GGCCCTTTCG 
AGCAACTTCC ACAGTGGATT GTTGAGTACC GCACAAAGTT ATCCTAGTCT CTCGAGTTCG 
AGTTCGAATA ATGCAAATTC GGTCGCGGTA AGTGGAGGAA CCAACAACAA TTCAGCGAGC 
GGAGTCGGTT CCAACAATGG ATCCGTTCAA AGCGGAAATG TCCAAGCCGT GTTGAATCCA 
GCACTGACGA TGAGTCTCAC CTCAACCAGT AGCGATAGTG AGCAGGTGTC TTTGGAAGAT 
TTCCTTGAAC AGTGCCGAGC TCCAACATTG CTGGGAGATT TAGACGACGA CGAAGACATG 
GAGGACGAGA ATGATGACGA TGAAAATGAG GATGAATACG AAGAAGTCGG AAATACTTTG 
CTTCAAGTGA TGGTTTCTCG TAATTTGTTA TCGTTTATGG ATGAGGAGAC TTTGGAGAAT 
CGTCTGGCTG CCGCTGGCAA ACGCAAATCG TGGGACGACG AATTCGTGCT TAAACGCCAA 
TTCTCGGCAC TCATTCCTGC ATTCGATCCG CGTCCTGGCA GGACCAACGT CAATCAAACA 
AGTGATCTGG AAATACCTGC TCCCGGCAGT TCTACAGACA GTAGTCATCC GAGTAGCAGC 
CACTCTGAGC ATGCTCCTTT GCCTCAACCG TCCCTTGCAC TTCTTCTGCG TGGTCCCAAC 
ATAAACGGAG TAAATGATGT GGAAATTCCA CTGTCGCAGC CCGATTGGAC AATATTCCGA 
GCGGTCCAAG AACTGATCCT TCAAACCAAC ATGACCAAGC AGGATAAGTT CCGCAAAATT 
TGGCAACCAA CGTACACCAT CGTCTACAGG GAAGCCTCTT CTTCGGCCGG ACTCGGTTCC 
TGCCGCGGTG AGGACTTCAG CAGCGGAGAA GAAGGACGGG CCACCCCCGT CGTTTCCATG 
TTTTCCCAAC GCAGTGGAGG ATCTACCCTT TCGCCTAGCT CTCCGATCCC CGGAACACCA 
CTCAATCCAA CCGCCACCGC TCACTGCACT GTGGACGACG TCTTGCAGCT TCTCGGCCAA 
TTGAATGCCA TCAATCGAAC GCTTACATCT TCTCCATCCA ACAACGACAA GAACCTCATC 
CCGGACATGG AATCCAACAA TCTCAACGCG GATATATTCT TGAGCAAGAA AATTACCAAC 
AAACTGCAGC AGCAAATCCA AGACCCACTA GTCCTGGCGA GTGGAAGCCT GCCGAAATGG 
TGCGAAGACT TCAACCAAAG CTGTCCGTTC CTGTTCCCGT TCGAGACGCG GCAGCTGTAC 
TTCAACTGTA CGGCTTTCGG CGCCTCGAGG AGCATCGTGT GGCTGCAGTC GCAGCGGGAT 
GTGAACCTGG AACGGCAACG GGCTCCCGGG TTGAGCCCAC GGCATGCCGA TCAGCACGAG 
TTCCGCGTTG GGCGGTTGAA GCACGAGCGC GTCAAGGTTC CACGCGGTGA GAATCTACTC 
GAATGGGCAC AACAGGTTAT GAAAGTTCAC TGTAATCGCA AGTCGGTGTT GGAGGTGGAA 
TTCGTTGGAG AGGAAGGCAC TGGATTGGGT CCGACTTTGG AATTCTACGC CCTGGTTGCA 
GCTGAACTGC AGCGAAGTGA TCTGGGAATG TGGCTGTGCG ACGATGAACC TAAGCTAATC 
GAAGACGAAA TCGATCTAGG AGAGGGAAGC AAACCCATCG GTTATTACGT TCGTCGTTCA 
ACCGGATTGT TCCCGGCGCC GTTGCCACAG GAATCTGAAA TTTGTGACTT TGTGTCAAAC 
TACTTCTGGT TCTTGGGAGT GTTTCTGGCG AAGGTACTGC AGGACGGTCG TCTAGTCGAT 
CTGCCACTGT CGAACAGCTT CTTACAACTG TTATGTAATA ACAAATCTCT CGCTAGAGGT 
TCACTAAGCG AACTTTCTTC CAAGTCTGCC TTGCACGATG ATGTGATGAT TTCTTCTTTA 
ATGTCCGAGG AAAGCGATCG TGATCTGGTG GATTCATATC AGTCAAAATT GGCTAACGAT 
GGCTGTTGGT ATGATGGAAT CCTGTCCCAA GAAAATCTCC AAGAAATCGA TCCGATTCGG 
TACGAGTTCT TGAAAGAACT GCAAGAGTTG GTGCAGCAGA AACAAAATAT CGAACAAAAT 
GATGATCTCA CCTCCGAAGA GAAGCTACTG CAAATTAGCG AGCTTAAATT CAACACCAAA 
ACTGGCAGTG TAGCTTTGGA GGACCTCGCT CTAACGTTCA CCTATCTACC AAGTTCGAAG 
AACTACGGCT ACCAATCGGC AGATCTGATT CCAAACGGTT CCAACATTGA TGTAACGATC 
AACAACGTCG AAGAGTACTG CAATTTGACG ATCAACTTCT GCCTGCAAGA AGGCATTGCT 
AAGCAACTTA CTGCGTTCCA TCGAGGTTTC TGTGAAGTGT TCCCGCTGAA CAAATTGGCC 
GCTTTCACAT CGGAAGAAAT CCGCAAGATG CTTTGTGGCG AGCAGAACCC AGAGTGGACA 
CGGGAGGACA TTATGACCTA CACGGAACCG AAACTCGGAT ACTCCAAGGA AAGCCCTGGA 
TTCCTGCGTT TCGTGAACGT GTTGATGGGG ATGAATGCCA GCGAAAGGAA AGCATTTTTG 
CAGTTCACCA CCGGCTGCAG CAGTTTACCA CCCGGTGGGC TGGCCAATTT GCACCCGCGG 
CTGACAGTGG TGCGGAAGGT AGATGCCGGC GAAGGTTCCT ACCCTTCGGT CAACACCTGT 
GTCCACTACC TCAAATTGCC GGACTATCCG AATGAGGAAA TCTTGCGGGA ACGATTGCTG 
ACGGCCACAA AAGAAAAGGG ATTCCATCTT AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021704015.1
CDS	98..8833
Translation	MGDVDPETLLEWLSMGQGDERDMQLIALEQLCMLLLMSDNVDRCFESCPPRTFLPALCKIFLDELAPENVLEVTARAITYYLDVSSECTRRIVAIDGAIKAICNRLVVADLESRTSRDLAEQCIKVLELICTREAGAVFEGGGLNCVLSFIRDNGSQIHKDTLHSAMAVVSRLCTKVEPQGANVQTCVESLSTLLQHEDPLVADGALKCFASVADRFTRKGVDPAPLAEYGLVTELLNRLSNAAGPQTSALTAGASSNATPAAQVNSQETTGTVQLSSSAPKTQGAAEAGRSSQSIATTISLLSTLCRGSPSITHDLLRSNLPDAMERAFKGDERCVLDCMRLADLILLLLFEGRQALGRVGGSSGQLAPRVRRADSSTERTHRQLIDCIRSKDTEALIEAIESGGIDVNCMDDVGQTLLNWASAFGTLEMVEFLCDKGADVNKGQRSSSLHYAACFGRPGIAKVLLKHGANPDLRDEDGKTPLDKARERPDEGHREVASILQSPGEWMTAATRSDLKTDPEDGESEPRGDPEMAPVYLKFFLPIFCKTFQSTMLASVRRSSLGLIKKMIQYVQPEMLSRLCSSEGLQSHEHSLGTLLVEVVASVLDNEISYSWPSQPSAPSLLPLTVSSSSTNTTSSNSILAKYSCADRVSKYVQNLRLQQNQQQPPERRRNSSSYIIPPPPPMLPLTSSQDDEDGHLVVLTIIEELMSKTQDDFLDHFARLGVFSKVQALMGENGFSGGEGDNNDVIKSQDELKPSATGAQDASVVSTTGSSSSGTATTNTVEDAKEILPGKAYHWRDWSICRGRDCLYVWSDSAALELSNGSNGWFRFILDGKLATMYSSGSPENGSDSSGKGRTADSLAGEENRGEFLEKLQRARGAVRQGTPSQPILSSPSLSRIVVGNWVLQSQKEHQLHINNSEGHQVTILQDELPGFIFESNRGTKHTFTAETTLGPDFAAGWINTKKKKMRSKAEAQKYQVKNIARDLYNRYFKAAQAVPRGAVAKLCTIVRQIEAALEEQCAPKASSLLQVRIQPDASKATNSTWQEKLYNALHDLVQLLNDDGVISAYEMHSSGLVQSLVAVLSRNYWELEMNRSKANKYQKQRISIFKKCMYGDTKNGKNTASILVQKLVSVLESIEKLPVHMYDSPGGSYGLQILTKRLSFRLERAACEQTLFDRTGRNLKMEPLATVGQLNKYLLKMVAKQWYDMDRSSFLYLKKLKEAKPGSVHFKHQHDFDENGLVYYIGTNGKTTEWVNPAQYGLVTVTSSEGKQLPYGKLEDILSRDSVSVNCHTKDNKKSWFAIDLGMFIIPTAYTLRHARGYGRSALRNWMFQMSKDGINWATMLTHSDDKSLAEPGSTCTWPLECSAEEQQGWRHVRIHQNGRNASGQTHYLSLSGFEIYGKVVSVCDDMGKAAAKENEARLRKERRQIRAQLKYITQGARVVRGVDWHWDDQDGSPPGEGTVTGEIHNGWIDVKWDHGLRNSYRMGAEGKYDLKLSNSENLTAPYDMNNSGAGLVPINAGSVSSAKKVYDKSLNVLTSRKSSSTPSLPEATENKSSVASTEQATSVDNLAWKQAVEVIAENVLSCARSDLANTSGGSSSNDLSAPGSNNNNLNNQEVSVIVHSLGERGNIPDLSQINTSTSTLVSDLATITENLTLSDNIKNNISIGSSTQFVSNFGTQLAACSSSSSSEDNNKTNNINETNNKINLNNSSSSSSGKTAYLPTKLDVLDKMREGVDMLRNNTNNLLSSELLTQSNLLSSVKIALPTPQQPQQTGATAPSGPFFVASTSTSSTSTLPSGDKIVGDVKFNNTINNNTASGGVTKKVLNEVKQQEPDDRDIANNLKNNTIVVDSVEVGAAGSSKESTAESPSAAVTVNPMSVSVPNLTSNDNNASQEVQTPPGLLETFAAIARRRTSGSSVSHHVNPSSNASTASSGTSGTNNAQPNNQLSSLGSNIQAANSSFFPRGQNSVTSLVKLALSSNFHSGLLSTAQSYPSLSSSSSNNANSVAVSGGTNNNSASGVGSNNGSVQSGNVQAVLNPALTMSLTSTSSDSEQVSLEDFLEQCRAPTLLGDLDDDEDMEDENDDDENEDEYEEVGNTLLQVMVSRNLLSFMDEETLENRLAAAGKRKSWDDEFVLKRQFSALIPAFDPRPGRTNVNQTSDLEIPAPGSSTDSSHPSSSHSEHAPLPQPSLALLLRGPNINGVNDVEIPLSQPDWTIFRAVQELILQTNMTKQDKFRKIWQPTYTIVYREASSSAGLGSCRGEDFSSGEEGRATPVVSMFSQRSGGSTLSPSSPIPGTPLNPTATAHCTVDDVLQLLGQLNAINRTLTSSPSNNDKNLIPDMESNNLNADIFLSKKITNKLQQQIQDPLVLASGSLPKWCEDFNQSCPFLFPFETRQLYFNCTAFGASRSIVWLQSQRDVNLERQRAPGLSPRHADQHEFRVGRLKHERVKVPRGENLLEWAQQVMKVHCNRKSVLEVEFVGEEGTGLGPTLEFYALVAAELQRSDLGMWLCDDEPKLIEDEIDLGEGSKPIGYYVRRSTGLFPAPLPQESEICDFVSNYFWFLGVFLAKVLQDGRLVDLPLSNSFLQLLCNNKSLARGSLSELSSKSALHDDVMISSLMSEESDRDLVDSYQSKLANDGCWYDGILSQENLQEIDPIRYEFLKELQELVQQKQNIEQNDDLTSEEKLLQISELKFNTKTGSVALEDLALTFTYLPSSKNYGYQSADLIPNGSNIDVTINNVEEYCNLTINFCLQEGIAKQLTAFHRGFCEVFPLNKLAAFTSEEIRKMLCGEQNPEWTREDIMTYTEPKLGYSKESPGFLRFVNVLMGMNASERKAFLQFTTGCSSLPPGGLANLHPRLTVVRKVDAGEGSYPSVNTCVHYLKLPDYPNEEILRERLLTATKEKGFHLN

Target ORF information:

RefSeq Version	XM_021848323.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848323.1

ORF Insert Sequence:

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGATA AGCTACAGCT GGCCGTCACA GCCATCAGCA 
CCATCTCTAC TGCCCTTAAC CGTTTCCTCC TCTTCTACCA ATACTACTAG TAGCAATAGT 
ATTCTAGCCA AATATAGTTG CGCTGACCGA GTAAGCAAGT ATGTCCAGAA CCTGCGACTG 
CAACAAAACC AGCAGCAGCC ACCAGAGCGG CGTCGTAACT CTAGTTCTTA CATAATTCCA 
CCGCCGCCGC CAATGCTGCC GTTGACCTCC TCCCAGGACG ACGAAGATGG CCATCTGGTG 
GTGCTTACCA TTATCGAAGA GCTCATGTCC AAGACGCAAG ACGATTTCCT CGATCACTTT 
GCCCGGCTCG GAGTGTTCTC CAAAGTTCAG GCCCTGATGG GAGAAAACGG CTTCAGCGGG 
GGCGAAGGTG ATAACAACGA TGTGATCAAA TCCCAAGACG AGCTCAAACC GTCTGCGACC 
GGAGCACAAG ATGCTTCGGT TGTCTCCACT ACTGGAAGCT CCTCCTCTGG AACTGCCACT 
ACTAATACGG TGGAAGATGC CAAAGAAATC CTTCCCGGGA AGGCGTACCA CTGGCGAGAC 
TGGAGTATTT GTCGAGGTCG CGACTGCTTG TACGTATGGT CGGATTCAGC CGCCTTGGAG 
CTTTCGAACG GATCCAATGG GTGGTTCCGG TTCATCCTAG ACGGGAAATT GGCCACTATG 
TACTCCAGTG GTAGTCCAGA GAATGGCAGT GATAGCTCAG GCAAGGGAAG GACAGCGGAT 
TCCTTAGCCG GCGAAGAAAA CCGTGGTGAA TTCCTAGAAA AATTACAACG AGCTCGCGGA 
GCGGTACGGC AGGGAACTCC CTCCCAGCCG ATTCTTTCGT CGCCGAGCCT TTCGCGAATC 
GTGGTCGGCA ATTGGGTGCT GCAGAGCCAA AAAGAGCATC AGCTACATAT CAACAACTCC 
GAAGGTCACC AGGTGACGAT CCTGCAGGAC GAACTGCCCG GATTCATCTT CGAGAGCAAT 
CGCGGCACGA AGCATACTTT CACGGCGGAA ACCACTCTAG GACCGGACTT CGCCGCCGGA 
TGGATCAACA CCAAGAAGAA GAAGATGCGT TCGAAAGCGG AGGCTCAGAA ATATCAGGTT 
AAAAACATCG CCCGAGATCT GTACAACCGG TACTTCAAAG CAGCTCAAGC CGTCCCGCGG 
GGCGCCGTGG CAAAACTGTG CACCATCGTG CGTCAAATCG AGGCAGCCCT GGAGGAACAA 
TGTGCCCCTA AGGCGTCCAG TTTGCTGCAG GTTCGGATTC AACCTGACGC TTCTAAAGCA 
ACGAACAGCA CATGGCAGGA GAAGCTGTAC AACGCTCTGC ACGATCTTGT GCAACTGTTG 
AACGACGATG GAGTCATCAG TGCTTACGAG ATGCACAGTT CCGGACTTGT ACAATCCTTG 
GTGGCCGTGC TGTCCCGGAA CTACTGGGAA CTGGAGATGA ACCGGAGCAA GGCCAACAAG 
TACCAGAAGC AACGGATATC CATTTTCAAG AAGTGCATGT ATGGCGATAC CAAAAATGGC 
AAAAACACGG CCAGCATTCT GGTGCAGAAG TTGGTATCTG TATTGGAGAG CATTGAGAAG 
CTTCCGGTGC ACATGTATGA CTCCCCAGGG GGTAGCTATG GGTTGCAGAT TCTCACAAAG 
CGACTGAGCT TCCGACTTGA GCGGGCAGCT TGTGAGCAAA CTCTCTTCGA TAGGACCGGT 
AGAAACCTGA AAATGGAACC ATTGGCAACC GTTGGCCAGC TGAACAAGTA TCTTCTGAAA 
ATGGTAGCAA AGCAGTGGTA CGATATGGAT CGATCGTCCT TCTTGTACTT GAAAAAGCTG 
AAAGAGGCAA AACCAGGAAG TGTACACTTC AAGCACCAGC ACGATTTCGA CGAGAATGGT 
CTCGTGTACT ATATCGGGAC GAACGGAAAA ACCACGGAAT GGGTCAATCC TGCTCAGTAC 
GGATTGGTTA CTGTCACAAG TAGCGAAGGC AAACAGCTCC CGTACGGTAA GCTGGAAGAT 
ATCCTGTCGA GAGATAGCGT TAGCGTGAAT TGTCACACCA AGGATAACAA GAAATCGTGG 
TTCGCCATCG ATTTGGGAAT GTTTATCATT CCAACTGCTT ATACTTTGCG ACACGCCCGT 
GGCTATGGCC GCTCTGCTCT GAGAAATTGG ATGTTCCAGA TGTCAAAGGA TGGAATAAAT 
TGGGCGACAA TGTTGACCCA TTCGGATGAT AAAAGTTTGG CAGAACCCGG AAGCACTTGC 
ACTTGGCCAC TCGAATGCTC TGCCGAAGAA CAGCAGGGAT GGCGTCACGT GAGAATCCAT 
CAAAATGGAC GGAATGCTTC AGGGCAGACC CATTACCTTA GCTTGAGTGG ATTTGAAATC 
TACGGAAAGG TTGTCTCGGT TTGTGATGAT ATGGGAAAAG CTGCGGCCAA GGAGAATGAA 
GCTCGGCTTA GGAAAGAGCG ACGACAGATT CGAGCTCAGT TGAAGTATAT CACTCAAGGT 
GCACGCGTTG TTCGAGGAGT TGATTGGCAT TGGGATGATC AAGATGGAAG TCCTCCTGGA 
GAAGGAACCG TAACTGGTGA GATTCATAAC GGTTGGATCG ATGTAAAGTG GGACCATGGT 
TTAAGAAACT CCTATCGCAT GGGAGCCGAA GGCAAATATG ATCTGAAGCT GTCAAACAGT 
GAAAACCTGA CTGCACCTTA CGATATGAAC AACTCCGGTG CTGGACTTGT TCCAATTAAC 
GCAGGAAGCG TGTCTTCGGC GAAGAAAGTA TATGATAAGT CGTTGAACGT TCTGACCAGT 
CGCAAATCGA GCTCTACTCC GAGCCTCCCG GAAGCAACGG AGAACAAGTC ATCAGTAGCT 
TCGACTGAAC AAGCCACTTC CGTGGATAAC CTTGCATGGA AACAAGCCGT AGAAGTGATT 
GCCGAAAATG TGCTATCCTG TGCTCGATCT GATTTGGCCA ATACCAGTGG TGGAAGTAGC 
AGCAATGACT TATCCGCTCC CGGATCCAAC AATAATAATC TGAACAACCA GGAAGTGTCT 
GTGATTGTCC ACTCGTTGGG AGAGCGGGGA AACATCCCAG ATCTGTCGCA AATCAATACA 
AGTACTTCGA CTCTAGTCTC GGATTTGGCC ACCATCACGG AGAATCTAAC ACTCTCGGAT 
AACATCAAGA ACAACATCAG CATCGGAAGT AGCACCCAGT TTGTGAGCAA CTTTGGAACC 
CAACTTGCAG CTTGCTCATC CTCGTCTTCG TCCGAAGACA ACAACAAAAC CAACAACATC 
AATGAAACCA ATAACAAAAT CAATCTCAAC AACAGCAGCA GCAGTAGTTC CGGTAAAACG 
GCATACCTGC CAACCAAGCT CGACGTGTTG GACAAAATGA GGGAAGGCGT CGACATGCTT 
CGGAACAATA CCAACAATTT GCTGTCCTCG GAATTGCTCA CCCAGTCAAA TCTACTCTCG 
TCCGTAAAGA TTGCACTTCC GACGCCACAA CAACCGCAGC AAACTGGAGC GACAGCTCCC 
TCAGGACCTT TCTTCGTGGC CAGCACAAGC ACTAGCAGCA CGAGCACTCT CCCTTCGGGT 
GATAAAATCG TCGGAGATGT CAAGTTTAAC AATACCATCA ACAATAATAC CGCTTCCGGT 
GGCGTAACGA AGAAAGTCCT GAACGAAGTC AAACAGCAGG AACCGGACGA TCGCGACATT 
GCCAACAATT TGAAGAACAA CACCATTGTC GTCGATTCGG TGGAAGTTGG TGCAGCAGGA 
AGTTCGAAGG AATCAACTGC TGAGTCACCT TCGGCGGCGG TCACGGTCAA TCCGATGAGT 
GTGAGTGTCC CAAATCTGAC GAGCAATGAT AACAATGCTT CACAGGAGGT ACAAACTCCA 
CCAGGGTTGT TGGAAACGTT TGCGGCCATA GCTCGAAGGC GTACTTCTGG AAGCTCTGTA 
TCCCATCATG TGAACCCTAG CTCAAACGCT TCAACCGCTT CTTCCGGAAC CAGTGGCACA 
AACAATGCTC AACCCAACAA TCAGCTGAGT AGCCTTGGGT CCAATATTCA AGCAGCGAAC 
TCTAGCTTCT TCCCGAGGGG GCAAAACTCC GTTACTAGTC TGGTGAAATT GGCCCTTTCG 
AGCAACTTCC ACAGTGGATT GTTGAGTACC GCACAAAGTT ATCCTAGTCT CTCGAGTTCG 
AGTTCGAATA ATGCAAATTC GGTCGCGGTA AGTGGAGGAA CCAACAACAA TTCAGCGAGC 
GGAGTCGGTT CCAACAATGG ATCCGTTCAA AGCGGAAATG TCCAAGCCGT GTTGAATCCA 
GCACTGACGA TGAGTCTCAC CTCAACCAGT AGCGATAGTG AGCAGGTGTC TTTGGAAGAT 
TTCCTTGAAC AGTGCCGAGC TCCAACATTG CTGGGAGATT TAGACGACGA CGAAGACATG 
GAGGACGAGA ATGATGACGA TGAAAATGAG GATGAATACG AAGAAGTCGG AAATACTTTG 
CTTCAAGTGA TGGTTTCTCG TAATTTGTTA TCGTTTATGG ATGAGGAGAC TTTGGAGAAT 
CGTCTGGCTG CCGCTGGCAA ACGCAAATCG TGGGACGACG AATTCGTGCT TAAACGCCAA 
TTCTCGGCAC TCATTCCTGC ATTCGATCCG CGTCCTGGCA GGACCAACGT CAATCAAACA 
AGTGATCTGG AAATACCTGC TCCCGGCAGT TCTACAGACA GTAGTCATCC GAGTAGCAGC 
CACTCTGAGC ATGCTCCTTT GCCTCAACCG TCCCTTGCAC TTCTTCTGCG TGGTCCCAAC 
ATAAACGGAG TAAATGATGT GGAAATTCCA CTGTCGCAGC CCGATTGGAC AATATTCCGA 
GCGGTCCAAG AACTGATCCT TCAAACCAAC ATGACCAAGC AGGATAAGTT CCGCAAAATT 
TGGCAACCAA CGTACACCAT CGTCTACAGG GAAGCCTCTT CTTCGGCCGG ACTCGGTTCC 
TGCCGCGGTG AGGACTTCAG CAGCGGAGAA GAAGGACGGG CCACCCCCGT CGTTTCCATG 
TTTTCCCAAC GCAGTGGAGG ATCTACCCTT TCGCCTAGCT CTCCGATCCC CGGAACACCA 
CTCAATCCAA CCGCCACCGC TCACTGCACT GTGGACGACG TCTTGCAGCT TCTCGGCCAA 
TTGAATGCCA TCAATCGAAC GCTTACATCT TCTCCATCCA ACAACGACAA GAACCTCATC 
CCGGACATGG AATCCAACAA TCTCAACGCG GATATATTCT TGAGCAAGAA AATTACCAAC 
AAACTGCAGC AGCAAATCCA AGACCCACTA GTCCTGGCGA GTGGAAGCCT GCCGAAATGG 
TGCGAAGACT TCAACCAAAG CTGTCCGTTC CTGTTCCCGT TCGAGACGCG GCAGCTGTAC 
TTCAACTGTA CGGCTTTCGG CGCCTCGAGG AGCATCGTGT GGCTGCAGTC GCAGCGGGAT 
GTGAACCTGG AACGGCAACG GGCTCCCGGG TTGAGCCCAC GGCATGCCGA TCAGCACGAG 
TTCCGCGTTG GGCGGTTGAA GCACGAGCGC GTCAAGGTTC CACGCGGTGA GAATCTACTC 
GAATGGGCAC AACAGGTTAT GAAAGTTCAC TGTAATCGCA AGTCGGTGTT GGAGGTGGAA 
TTCGTTGGAG AGGAAGGCAC TGGATTGGGT CCGACTTTGG AATTCTACGC CCTGGTTGCA 
GCTGAACTGC AGCGAAGTGA TCTGGGAATG TGGCTGTGCG ACGATGAACC TAAGCTAATC 
GAAGACGAAA TCGATCTAGG AGAGGGAAGC AAACCCATCG GTTATTACGT TCGTCGTTCA 
ACCGGATTGT TCCCGGCGCC GTTGCCACAG GAATCTGAAA TTTGTGACTT TGTGTCAAAC 
TACTTCTGGT TCTTGGGAGT GTTTCTGGCG AAGGTACTGC AGGACGGTCG TCTAGTCGAT 
CTGCCACTGT CGAACAGCTT CTTACAACTG TTATGTAATA ACAAATCTCT CGCTAGAGGT 
TCACTAAGCG AACTTTCTTC CAAGTCTGCC TTGCACGATG ATGTGATGAT TTCTTCTTTA 
ATGTCCGAGG AAAGCGATCG TGATCTGGTG GATTCATATC AGTCAAAATT GGCTAACGAT 
GGCTGTTGGT ATGATGGAAT CCTGTCCCAA GAAAATCTCC AAGAAATCGA TCCGATTCGG 
TACGAGTTCT TGAAAGAACT GCAAGAGTTG GTGCAGCAGA AACAAAATAT CGAACAAAAT 
GATGATCTCA CCTCCGAAGA GAAGCTACTG CAAATTAGCG AGCTTAAATT CAACACCAAA 
ACTGGCAGTG TAGCTTTGGA GGACCTCGCT CTAACGTTCA CCTATCTACC AAGTTCGAAG 
AACTACGGCT ACCAATCGGC AGATCTGATT CCAAACGGTT CCAACATTGA TGTAACGATC 
AACAACGTCG AAGAGTACTG CAATTTGACG ATCAACTTCT GCCTGCAAGA AGGCATTGCT 
AAGCAACTTA CTGCGTTCCA TCGAGGTTTC TGTGAAGTGT TCCCGCTGAA CAAATTGGCC 
GCTTTCACAT CGGAAGAAAT CCGCAAGATG CTTTGTGGCG AGCAGAACCC AGAGTGGACA 
CGGGAGGACA TTATGACCTA CACGGAACCG AAACTCGGAT ACTCCAAGGA AAGCCCTGGA 
TTCCTGCGTT TCGTGAACGT GTTGATGGGG ATGAATGCCA GCGAAAGGAA AGCATTTTTG 
CAGTTCACCA CCGGCTGCAG CAGTTTACCA CCCGGTGGGC TGGCCAATTT GCACCCGCGG 
CTGACAGTGG TGCGGAAGGT AGATGCCGGC GAAGGTTCCT ACCCTTCGGT CAACACCTGT 
GTCCACTACC TCAAATTGCC GGACTATCCG AATGAGGAAA TCTTGCGGGA ACGATTGCTG 
ACGGCCACAA AAGAAAAGGG ATTCCATCTT AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL007705 ( XM_021848322.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021704014.1 Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X1, mRNA.

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CloneID	OAe124645
Clone ID Related Accession (Same CDS sequence)	XM_021848323.1 , XM_021848322.1
Accession Version	XM_021848322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGATA AGCTACAGCT GGCCGTCACA GCCATCAGCA 
CCATCTCTAC TGCCCTTAAC CGTTTCCTCC TCTTCTACCA ATACTACTAG TAGCAATAGT 
ATTCTAGCCA AATATAGTTG CGCTGACCGA GTAAGCAAGT ATGTCCAGAA CCTGCGACTG 
CAACAAAACC AGCAGCAGCC ACCAGAGCGG CGTCGTAACT CTAGTTCTTA CATAATTCCA 
CCGCCGCCGC CAATGCTGCC GTTGACCTCC TCCCAGGACG ACGAAGATGG CCATCTGGTG 
GTGCTTACCA TTATCGAAGA GCTCATGTCC AAGACGCAAG ACGATTTCCT CGATCACTTT 
GCCCGGCTCG GAGTGTTCTC CAAAGTTCAG GCCCTGATGG GAGAAAACGG CTTCAGCGGG 
GGCGAAGGTG ATAACAACGA TGTGATCAAA TCCCAAGACG AGCTCAAACC GTCTGCGACC 
GGAGCACAAG ATGCTTCGGT TGTCTCCACT ACTGGAAGCT CCTCCTCTGG AACTGCCACT 
ACTAATACGG TGGAAGATGC CAAAGAAATC CTTCCCGGGA AGGCGTACCA CTGGCGAGAC 
TGGAGTATTT GTCGAGGTCG CGACTGCTTG TACGTATGGT CGGATTCAGC CGCCTTGGAG 
CTTTCGAACG GATCCAATGG GTGGTTCCGG TTCATCCTAG ACGGGAAATT GGCCACTATG 
TACTCCAGTG GTAGTCCAGA GAATGGCAGT GATAGCTCAG GCAAGGGAAG GACAGCGGAT 
TCCTTAGCCG GCGAAGAAAA CCGTGGTGAA TTCCTAGAAA AATTACAACG AGCTCGCGGA 
GCGGTACGGC AGGGAACTCC CTCCCAGCCG ATTCTTTCGT CGCCGAGCCT TTCGCGAATC 
GTGGTCGGCA ATTGGGTGCT GCAGAGCCAA AAAGAGCATC AGCTACATAT CAACAACTCC 
GAAGGTCACC AGGTGACGAT CCTGCAGGAC GAACTGCCCG GATTCATCTT CGAGAGCAAT 
CGCGGCACGA AGCATACTTT CACGGCGGAA ACCACTCTAG GACCGGACTT CGCCGCCGGA 
TGGATCAACA CCAAGAAGAA GAAGATGCGT TCGAAAGCGG AGGCTCAGAA ATATCAGGTT 
AAAAACATCG CCCGAGATCT GTACAACCGG TACTTCAAAG CAGCTCAAGC CGTCCCGCGG 
GGCGCCGTGG CAAAACTGTG CACCATCGTG CGTCAAATCG AGGCAGCCCT GGAGGAACAA 
TGTGCCCCTA AGGCGTCCAG TTTGCTGCAG GTTCGGATTC AACCTGACGC TTCTAAAGCA 
ACGAACAGCA CATGGCAGGA GAAGCTGTAC AACGCTCTGC ACGATCTTGT GCAACTGTTG 
AACGACGATG GAGTCATCAG TGCTTACGAG ATGCACAGTT CCGGACTTGT ACAATCCTTG 
GTGGCCGTGC TGTCCCGGAA CTACTGGGAA CTGGAGATGA ACCGGAGCAA GGCCAACAAG 
TACCAGAAGC AACGGATATC CATTTTCAAG AAGTGCATGT ATGGCGATAC CAAAAATGGC 
AAAAACACGG CCAGCATTCT GGTGCAGAAG TTGGTATCTG TATTGGAGAG CATTGAGAAG 
CTTCCGGTGC ACATGTATGA CTCCCCAGGG GGTAGCTATG GGTTGCAGAT TCTCACAAAG 
CGACTGAGCT TCCGACTTGA GCGGGCAGCT TGTGAGCAAA CTCTCTTCGA TAGGACCGGT 
AGAAACCTGA AAATGGAACC ATTGGCAACC GTTGGCCAGC TGAACAAGTA TCTTCTGAAA 
ATGGTAGCAA AGCAGTGGTA CGATATGGAT CGATCGTCCT TCTTGTACTT GAAAAAGCTG 
AAAGAGGCAA AACCAGGAAG TGTACACTTC AAGCACCAGC ACGATTTCGA CGAGAATGGT 
CTCGTGTACT ATATCGGGAC GAACGGAAAA ACCACGGAAT GGGTCAATCC TGCTCAGTAC 
GGATTGGTTA CTGTCACAAG TAGCGAAGGC AAACAGCTCC CGTACGGTAA GCTGGAAGAT 
ATCCTGTCGA GAGATAGCGT TAGCGTGAAT TGTCACACCA AGGATAACAA GAAATCGTGG 
TTCGCCATCG ATTTGGGAAT GTTTATCATT CCAACTGCTT ATACTTTGCG ACACGCCCGT 
GGCTATGGCC GCTCTGCTCT GAGAAATTGG ATGTTCCAGA TGTCAAAGGA TGGAATAAAT 
TGGGCGACAA TGTTGACCCA TTCGGATGAT AAAAGTTTGG CAGAACCCGG AAGCACTTGC 
ACTTGGCCAC TCGAATGCTC TGCCGAAGAA CAGCAGGGAT GGCGTCACGT GAGAATCCAT 
CAAAATGGAC GGAATGCTTC AGGGCAGACC CATTACCTTA GCTTGAGTGG ATTTGAAATC 
TACGGAAAGG TTGTCTCGGT TTGTGATGAT ATGGGAAAAG CTGCGGCCAA GGAGAATGAA 
GCTCGGCTTA GGAAAGAGCG ACGACAGATT CGAGCTCAGT TGAAGTATAT CACTCAAGGT 
GCACGCGTTG TTCGAGGAGT TGATTGGCAT TGGGATGATC AAGATGGAAG TCCTCCTGGA 
GAAGGAACCG TAACTGGTGA GATTCATAAC GGTTGGATCG ATGTAAAGTG GGACCATGGT 
TTAAGAAACT CCTATCGCAT GGGAGCCGAA GGCAAATATG ATCTGAAGCT GTCAAACAGT 
GAAAACCTGA CTGCACCTTA CGATATGAAC AACTCCGGTG CTGGACTTGT TCCAATTAAC 
GCAGGAAGCG TGTCTTCGGC GAAGAAAGTA TATGATAAGT CGTTGAACGT TCTGACCAGT 
CGCAAATCGA GCTCTACTCC GAGCCTCCCG GAAGCAACGG AGAACAAGTC ATCAGTAGCT 
TCGACTGAAC AAGCCACTTC CGTGGATAAC CTTGCATGGA AACAAGCCGT AGAAGTGATT 
GCCGAAAATG TGCTATCCTG TGCTCGATCT GATTTGGCCA ATACCAGTGG TGGAAGTAGC 
AGCAATGACT TATCCGCTCC CGGATCCAAC AATAATAATC TGAACAACCA GGAAGTGTCT 
GTGATTGTCC ACTCGTTGGG AGAGCGGGGA AACATCCCAG ATCTGTCGCA AATCAATACA 
AGTACTTCGA CTCTAGTCTC GGATTTGGCC ACCATCACGG AGAATCTAAC ACTCTCGGAT 
AACATCAAGA ACAACATCAG CATCGGAAGT AGCACCCAGT TTGTGAGCAA CTTTGGAACC 
CAACTTGCAG CTTGCTCATC CTCGTCTTCG TCCGAAGACA ACAACAAAAC CAACAACATC 
AATGAAACCA ATAACAAAAT CAATCTCAAC AACAGCAGCA GCAGTAGTTC CGGTAAAACG 
GCATACCTGC CAACCAAGCT CGACGTGTTG GACAAAATGA GGGAAGGCGT CGACATGCTT 
CGGAACAATA CCAACAATTT GCTGTCCTCG GAATTGCTCA CCCAGTCAAA TCTACTCTCG 
TCCGTAAAGA TTGCACTTCC GACGCCACAA CAACCGCAGC AAACTGGAGC GACAGCTCCC 
TCAGGACCTT TCTTCGTGGC CAGCACAAGC ACTAGCAGCA CGAGCACTCT CCCTTCGGGT 
GATAAAATCG TCGGAGATGT CAAGTTTAAC AATACCATCA ACAATAATAC CGCTTCCGGT 
GGCGTAACGA AGAAAGTCCT GAACGAAGTC AAACAGCAGG AACCGGACGA TCGCGACATT 
GCCAACAATT TGAAGAACAA CACCATTGTC GTCGATTCGG TGGAAGTTGG TGCAGCAGGA 
AGTTCGAAGG AATCAACTGC TGAGTCACCT TCGGCGGCGG TCACGGTCAA TCCGATGAGT 
GTGAGTGTCC CAAATCTGAC GAGCAATGAT AACAATGCTT CACAGGAGGT ACAAACTCCA 
CCAGGGTTGT TGGAAACGTT TGCGGCCATA GCTCGAAGGC GTACTTCTGG AAGCTCTGTA 
TCCCATCATG TGAACCCTAG CTCAAACGCT TCAACCGCTT CTTCCGGAAC CAGTGGCACA 
AACAATGCTC AACCCAACAA TCAGCTGAGT AGCCTTGGGT CCAATATTCA AGCAGCGAAC 
TCTAGCTTCT TCCCGAGGGG GCAAAACTCC GTTACTAGTC TGGTGAAATT GGCCCTTTCG 
AGCAACTTCC ACAGTGGATT GTTGAGTACC GCACAAAGTT ATCCTAGTCT CTCGAGTTCG 
AGTTCGAATA ATGCAAATTC GGTCGCGGTA AGTGGAGGAA CCAACAACAA TTCAGCGAGC 
GGAGTCGGTT CCAACAATGG ATCCGTTCAA AGCGGAAATG TCCAAGCCGT GTTGAATCCA 
GCACTGACGA TGAGTCTCAC CTCAACCAGT AGCGATAGTG AGCAGGTGTC TTTGGAAGAT 
TTCCTTGAAC AGTGCCGAGC TCCAACATTG CTGGGAGATT TAGACGACGA CGAAGACATG 
GAGGACGAGA ATGATGACGA TGAAAATGAG GATGAATACG AAGAAGTCGG AAATACTTTG 
CTTCAAGTGA TGGTTTCTCG TAATTTGTTA TCGTTTATGG ATGAGGAGAC TTTGGAGAAT 
CGTCTGGCTG CCGCTGGCAA ACGCAAATCG TGGGACGACG AATTCGTGCT TAAACGCCAA 
TTCTCGGCAC TCATTCCTGC ATTCGATCCG CGTCCTGGCA GGACCAACGT CAATCAAACA 
AGTGATCTGG AAATACCTGC TCCCGGCAGT TCTACAGACA GTAGTCATCC GAGTAGCAGC 
CACTCTGAGC ATGCTCCTTT GCCTCAACCG TCCCTTGCAC TTCTTCTGCG TGGTCCCAAC 
ATAAACGGAG TAAATGATGT GGAAATTCCA CTGTCGCAGC CCGATTGGAC AATATTCCGA 
GCGGTCCAAG AACTGATCCT TCAAACCAAC ATGACCAAGC AGGATAAGTT CCGCAAAATT 
TGGCAACCAA CGTACACCAT CGTCTACAGG GAAGCCTCTT CTTCGGCCGG ACTCGGTTCC 
TGCCGCGGTG AGGACTTCAG CAGCGGAGAA GAAGGACGGG CCACCCCCGT CGTTTCCATG 
TTTTCCCAAC GCAGTGGAGG ATCTACCCTT TCGCCTAGCT CTCCGATCCC CGGAACACCA 
CTCAATCCAA CCGCCACCGC TCACTGCACT GTGGACGACG TCTTGCAGCT TCTCGGCCAA 
TTGAATGCCA TCAATCGAAC GCTTACATCT TCTCCATCCA ACAACGACAA GAACCTCATC 
CCGGACATGG AATCCAACAA TCTCAACGCG GATATATTCT TGAGCAAGAA AATTACCAAC 
AAACTGCAGC AGCAAATCCA AGACCCACTA GTCCTGGCGA GTGGAAGCCT GCCGAAATGG 
TGCGAAGACT TCAACCAAAG CTGTCCGTTC CTGTTCCCGT TCGAGACGCG GCAGCTGTAC 
TTCAACTGTA CGGCTTTCGG CGCCTCGAGG AGCATCGTGT GGCTGCAGTC GCAGCGGGAT 
GTGAACCTGG AACGGCAACG GGCTCCCGGG TTGAGCCCAC GGCATGCCGA TCAGCACGAG 
TTCCGCGTTG GGCGGTTGAA GCACGAGCGC GTCAAGGTTC CACGCGGTGA GAATCTACTC 
GAATGGGCAC AACAGGTTAT GAAAGTTCAC TGTAATCGCA AGTCGGTGTT GGAGGTGGAA 
TTCGTTGGAG AGGAAGGCAC TGGATTGGGT CCGACTTTGG AATTCTACGC CCTGGTTGCA 
GCTGAACTGC AGCGAAGTGA TCTGGGAATG TGGCTGTGCG ACGATGAACC TAAGCTAATC 
GAAGACGAAA TCGATCTAGG AGAGGGAAGC AAACCCATCG GTTATTACGT TCGTCGTTCA 
ACCGGATTGT TCCCGGCGCC GTTGCCACAG GAATCTGAAA TTTGTGACTT TGTGTCAAAC 
TACTTCTGGT TCTTGGGAGT GTTTCTGGCG AAGGTACTGC AGGACGGTCG TCTAGTCGAT 
CTGCCACTGT CGAACAGCTT CTTACAACTG TTATGTAATA ACAAATCTCT CGCTAGAGGT 
TCACTAAGCG AACTTTCTTC CAAGTCTGCC TTGCACGATG ATGTGATGAT TTCTTCTTTA 
ATGTCCGAGG AAAGCGATCG TGATCTGGTG GATTCATATC AGTCAAAATT GGCTAACGAT 
GGCTGTTGGT ATGATGGAAT CCTGTCCCAA GAAAATCTCC AAGAAATCGA TCCGATTCGG 
TACGAGTTCT TGAAAGAACT GCAAGAGTTG GTGCAGCAGA AACAAAATAT CGAACAAAAT 
GATGATCTCA CCTCCGAAGA GAAGCTACTG CAAATTAGCG AGCTTAAATT CAACACCAAA 
ACTGGCAGTG TAGCTTTGGA GGACCTCGCT CTAACGTTCA CCTATCTACC AAGTTCGAAG 
AACTACGGCT ACCAATCGGC AGATCTGATT CCAAACGGTT CCAACATTGA TGTAACGATC 
AACAACGTCG AAGAGTACTG CAATTTGACG ATCAACTTCT GCCTGCAAGA AGGCATTGCT 
AAGCAACTTA CTGCGTTCCA TCGAGGTTTC TGTGAAGTGT TCCCGCTGAA CAAATTGGCC 
GCTTTCACAT CGGAAGAAAT CCGCAAGATG CTTTGTGGCG AGCAGAACCC AGAGTGGACA 
CGGGAGGACA TTATGACCTA CACGGAACCG AAACTCGGAT ACTCCAAGGA AAGCCCTGGA 
TTCCTGCGTT TCGTGAACGT GTTGATGGGG ATGAATGCCA GCGAAAGGAA AGCATTTTTG 
CAGTTCACCA CCGGCTGCAG CAGTTTACCA CCCGGTGGGC TGGCCAATTT GCACCCGCGG 
CTGACAGTGG TGCGGAAGGT AGATGCCGGC GAAGGTTCCT ACCCTTCGGT CAACACCTGT 
GTCCACTACC TCAAATTGCC GGACTATCCG AATGAGGAAA TCTTGCGGGA ACGATTGCTG 
ACGGCCACAA AAGAAAAGGG ATTCCATCTT AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021704014.1
CDS	934..9669
Translation	MGDVDPETLLEWLSMGQGDERDMQLIALEQLCMLLLMSDNVDRCFESCPPRTFLPALCKIFLDELAPENVLEVTARAITYYLDVSSECTRRIVAIDGAIKAICNRLVVADLESRTSRDLAEQCIKVLELICTREAGAVFEGGGLNCVLSFIRDNGSQIHKDTLHSAMAVVSRLCTKVEPQGANVQTCVESLSTLLQHEDPLVADGALKCFASVADRFTRKGVDPAPLAEYGLVTELLNRLSNAAGPQTSALTAGASSNATPAAQVNSQETTGTVQLSSSAPKTQGAAEAGRSSQSIATTISLLSTLCRGSPSITHDLLRSNLPDAMERAFKGDERCVLDCMRLADLILLLLFEGRQALGRVGGSSGQLAPRVRRADSSTERTHRQLIDCIRSKDTEALIEAIESGGIDVNCMDDVGQTLLNWASAFGTLEMVEFLCDKGADVNKGQRSSSLHYAACFGRPGIAKVLLKHGANPDLRDEDGKTPLDKARERPDEGHREVASILQSPGEWMTAATRSDLKTDPEDGESEPRGDPEMAPVYLKFFLPIFCKTFQSTMLASVRRSSLGLIKKMIQYVQPEMLSRLCSSEGLQSHEHSLGTLLVEVVASVLDNEISYSWPSQPSAPSLLPLTVSSSSTNTTSSNSILAKYSCADRVSKYVQNLRLQQNQQQPPERRRNSSSYIIPPPPPMLPLTSSQDDEDGHLVVLTIIEELMSKTQDDFLDHFARLGVFSKVQALMGENGFSGGEGDNNDVIKSQDELKPSATGAQDASVVSTTGSSSSGTATTNTVEDAKEILPGKAYHWRDWSICRGRDCLYVWSDSAALELSNGSNGWFRFILDGKLATMYSSGSPENGSDSSGKGRTADSLAGEENRGEFLEKLQRARGAVRQGTPSQPILSSPSLSRIVVGNWVLQSQKEHQLHINNSEGHQVTILQDELPGFIFESNRGTKHTFTAETTLGPDFAAGWINTKKKKMRSKAEAQKYQVKNIARDLYNRYFKAAQAVPRGAVAKLCTIVRQIEAALEEQCAPKASSLLQVRIQPDASKATNSTWQEKLYNALHDLVQLLNDDGVISAYEMHSSGLVQSLVAVLSRNYWELEMNRSKANKYQKQRISIFKKCMYGDTKNGKNTASILVQKLVSVLESIEKLPVHMYDSPGGSYGLQILTKRLSFRLERAACEQTLFDRTGRNLKMEPLATVGQLNKYLLKMVAKQWYDMDRSSFLYLKKLKEAKPGSVHFKHQHDFDENGLVYYIGTNGKTTEWVNPAQYGLVTVTSSEGKQLPYGKLEDILSRDSVSVNCHTKDNKKSWFAIDLGMFIIPTAYTLRHARGYGRSALRNWMFQMSKDGINWATMLTHSDDKSLAEPGSTCTWPLECSAEEQQGWRHVRIHQNGRNASGQTHYLSLSGFEIYGKVVSVCDDMGKAAAKENEARLRKERRQIRAQLKYITQGARVVRGVDWHWDDQDGSPPGEGTVTGEIHNGWIDVKWDHGLRNSYRMGAEGKYDLKLSNSENLTAPYDMNNSGAGLVPINAGSVSSAKKVYDKSLNVLTSRKSSSTPSLPEATENKSSVASTEQATSVDNLAWKQAVEVIAENVLSCARSDLANTSGGSSSNDLSAPGSNNNNLNNQEVSVIVHSLGERGNIPDLSQINTSTSTLVSDLATITENLTLSDNIKNNISIGSSTQFVSNFGTQLAACSSSSSSEDNNKTNNINETNNKINLNNSSSSSSGKTAYLPTKLDVLDKMREGVDMLRNNTNNLLSSELLTQSNLLSSVKIALPTPQQPQQTGATAPSGPFFVASTSTSSTSTLPSGDKIVGDVKFNNTINNNTASGGVTKKVLNEVKQQEPDDRDIANNLKNNTIVVDSVEVGAAGSSKESTAESPSAAVTVNPMSVSVPNLTSNDNNASQEVQTPPGLLETFAAIARRRTSGSSVSHHVNPSSNASTASSGTSGTNNAQPNNQLSSLGSNIQAANSSFFPRGQNSVTSLVKLALSSNFHSGLLSTAQSYPSLSSSSSNNANSVAVSGGTNNNSASGVGSNNGSVQSGNVQAVLNPALTMSLTSTSSDSEQVSLEDFLEQCRAPTLLGDLDDDEDMEDENDDDENEDEYEEVGNTLLQVMVSRNLLSFMDEETLENRLAAAGKRKSWDDEFVLKRQFSALIPAFDPRPGRTNVNQTSDLEIPAPGSSTDSSHPSSSHSEHAPLPQPSLALLLRGPNINGVNDVEIPLSQPDWTIFRAVQELILQTNMTKQDKFRKIWQPTYTIVYREASSSAGLGSCRGEDFSSGEEGRATPVVSMFSQRSGGSTLSPSSPIPGTPLNPTATAHCTVDDVLQLLGQLNAINRTLTSSPSNNDKNLIPDMESNNLNADIFLSKKITNKLQQQIQDPLVLASGSLPKWCEDFNQSCPFLFPFETRQLYFNCTAFGASRSIVWLQSQRDVNLERQRAPGLSPRHADQHEFRVGRLKHERVKVPRGENLLEWAQQVMKVHCNRKSVLEVEFVGEEGTGLGPTLEFYALVAAELQRSDLGMWLCDDEPKLIEDEIDLGEGSKPIGYYVRRSTGLFPAPLPQESEICDFVSNYFWFLGVFLAKVLQDGRLVDLPLSNSFLQLLCNNKSLARGSLSELSSKSALHDDVMISSLMSEESDRDLVDSYQSKLANDGCWYDGILSQENLQEIDPIRYEFLKELQELVQQKQNIEQNDDLTSEEKLLQISELKFNTKTGSVALEDLALTFTYLPSSKNYGYQSADLIPNGSNIDVTINNVEEYCNLTINFCLQEGIAKQLTAFHRGFCEVFPLNKLAAFTSEEIRKMLCGEQNPEWTREDIMTYTEPKLGYSKESPGFLRFVNVLMGMNASERKAFLQFTTGCSSLPPGGLANLHPRLTVVRKVDAGEGSYPSVNTCVHYLKLPDYPNEEILRERLLTATKEKGFHLN

Target ORF information:

RefSeq Version	XM_021848322.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848322.1

ORF Insert Sequence:

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGATA AGCTACAGCT GGCCGTCACA GCCATCAGCA 
CCATCTCTAC TGCCCTTAAC CGTTTCCTCC TCTTCTACCA ATACTACTAG TAGCAATAGT 
ATTCTAGCCA AATATAGTTG CGCTGACCGA GTAAGCAAGT ATGTCCAGAA CCTGCGACTG 
CAACAAAACC AGCAGCAGCC ACCAGAGCGG CGTCGTAACT CTAGTTCTTA CATAATTCCA 
CCGCCGCCGC CAATGCTGCC GTTGACCTCC TCCCAGGACG ACGAAGATGG CCATCTGGTG 
GTGCTTACCA TTATCGAAGA GCTCATGTCC AAGACGCAAG ACGATTTCCT CGATCACTTT 
GCCCGGCTCG GAGTGTTCTC CAAAGTTCAG GCCCTGATGG GAGAAAACGG CTTCAGCGGG 
GGCGAAGGTG ATAACAACGA TGTGATCAAA TCCCAAGACG AGCTCAAACC GTCTGCGACC 
GGAGCACAAG ATGCTTCGGT TGTCTCCACT ACTGGAAGCT CCTCCTCTGG AACTGCCACT 
ACTAATACGG TGGAAGATGC CAAAGAAATC CTTCCCGGGA AGGCGTACCA CTGGCGAGAC 
TGGAGTATTT GTCGAGGTCG CGACTGCTTG TACGTATGGT CGGATTCAGC CGCCTTGGAG 
CTTTCGAACG GATCCAATGG GTGGTTCCGG TTCATCCTAG ACGGGAAATT GGCCACTATG 
TACTCCAGTG GTAGTCCAGA GAATGGCAGT GATAGCTCAG GCAAGGGAAG GACAGCGGAT 
TCCTTAGCCG GCGAAGAAAA CCGTGGTGAA TTCCTAGAAA AATTACAACG AGCTCGCGGA 
GCGGTACGGC AGGGAACTCC CTCCCAGCCG ATTCTTTCGT CGCCGAGCCT TTCGCGAATC 
GTGGTCGGCA ATTGGGTGCT GCAGAGCCAA AAAGAGCATC AGCTACATAT CAACAACTCC 
GAAGGTCACC AGGTGACGAT CCTGCAGGAC GAACTGCCCG GATTCATCTT CGAGAGCAAT 
CGCGGCACGA AGCATACTTT CACGGCGGAA ACCACTCTAG GACCGGACTT CGCCGCCGGA 
TGGATCAACA CCAAGAAGAA GAAGATGCGT TCGAAAGCGG AGGCTCAGAA ATATCAGGTT 
AAAAACATCG CCCGAGATCT GTACAACCGG TACTTCAAAG CAGCTCAAGC CGTCCCGCGG 
GGCGCCGTGG CAAAACTGTG CACCATCGTG CGTCAAATCG AGGCAGCCCT GGAGGAACAA 
TGTGCCCCTA AGGCGTCCAG TTTGCTGCAG GTTCGGATTC AACCTGACGC TTCTAAAGCA 
ACGAACAGCA CATGGCAGGA GAAGCTGTAC AACGCTCTGC ACGATCTTGT GCAACTGTTG 
AACGACGATG GAGTCATCAG TGCTTACGAG ATGCACAGTT CCGGACTTGT ACAATCCTTG 
GTGGCCGTGC TGTCCCGGAA CTACTGGGAA CTGGAGATGA ACCGGAGCAA GGCCAACAAG 
TACCAGAAGC AACGGATATC CATTTTCAAG AAGTGCATGT ATGGCGATAC CAAAAATGGC 
AAAAACACGG CCAGCATTCT GGTGCAGAAG TTGGTATCTG TATTGGAGAG CATTGAGAAG 
CTTCCGGTGC ACATGTATGA CTCCCCAGGG GGTAGCTATG GGTTGCAGAT TCTCACAAAG 
CGACTGAGCT TCCGACTTGA GCGGGCAGCT TGTGAGCAAA CTCTCTTCGA TAGGACCGGT 
AGAAACCTGA AAATGGAACC ATTGGCAACC GTTGGCCAGC TGAACAAGTA TCTTCTGAAA 
ATGGTAGCAA AGCAGTGGTA CGATATGGAT CGATCGTCCT TCTTGTACTT GAAAAAGCTG 
AAAGAGGCAA AACCAGGAAG TGTACACTTC AAGCACCAGC ACGATTTCGA CGAGAATGGT 
CTCGTGTACT ATATCGGGAC GAACGGAAAA ACCACGGAAT GGGTCAATCC TGCTCAGTAC 
GGATTGGTTA CTGTCACAAG TAGCGAAGGC AAACAGCTCC CGTACGGTAA GCTGGAAGAT 
ATCCTGTCGA GAGATAGCGT TAGCGTGAAT TGTCACACCA AGGATAACAA GAAATCGTGG 
TTCGCCATCG ATTTGGGAAT GTTTATCATT CCAACTGCTT ATACTTTGCG ACACGCCCGT 
GGCTATGGCC GCTCTGCTCT GAGAAATTGG ATGTTCCAGA TGTCAAAGGA TGGAATAAAT 
TGGGCGACAA TGTTGACCCA TTCGGATGAT AAAAGTTTGG CAGAACCCGG AAGCACTTGC 
ACTTGGCCAC TCGAATGCTC TGCCGAAGAA CAGCAGGGAT GGCGTCACGT GAGAATCCAT 
CAAAATGGAC GGAATGCTTC AGGGCAGACC CATTACCTTA GCTTGAGTGG ATTTGAAATC 
TACGGAAAGG TTGTCTCGGT TTGTGATGAT ATGGGAAAAG CTGCGGCCAA GGAGAATGAA 
GCTCGGCTTA GGAAAGAGCG ACGACAGATT CGAGCTCAGT TGAAGTATAT CACTCAAGGT 
GCACGCGTTG TTCGAGGAGT TGATTGGCAT TGGGATGATC AAGATGGAAG TCCTCCTGGA 
GAAGGAACCG TAACTGGTGA GATTCATAAC GGTTGGATCG ATGTAAAGTG GGACCATGGT 
TTAAGAAACT CCTATCGCAT GGGAGCCGAA GGCAAATATG ATCTGAAGCT GTCAAACAGT 
GAAAACCTGA CTGCACCTTA CGATATGAAC AACTCCGGTG CTGGACTTGT TCCAATTAAC 
GCAGGAAGCG TGTCTTCGGC GAAGAAAGTA TATGATAAGT CGTTGAACGT TCTGACCAGT 
CGCAAATCGA GCTCTACTCC GAGCCTCCCG GAAGCAACGG AGAACAAGTC ATCAGTAGCT 
TCGACTGAAC AAGCCACTTC CGTGGATAAC CTTGCATGGA AACAAGCCGT AGAAGTGATT 
GCCGAAAATG TGCTATCCTG TGCTCGATCT GATTTGGCCA ATACCAGTGG TGGAAGTAGC 
AGCAATGACT TATCCGCTCC CGGATCCAAC AATAATAATC TGAACAACCA GGAAGTGTCT 
GTGATTGTCC ACTCGTTGGG AGAGCGGGGA AACATCCCAG ATCTGTCGCA AATCAATACA 
AGTACTTCGA CTCTAGTCTC GGATTTGGCC ACCATCACGG AGAATCTAAC ACTCTCGGAT 
AACATCAAGA ACAACATCAG CATCGGAAGT AGCACCCAGT TTGTGAGCAA CTTTGGAACC 
CAACTTGCAG CTTGCTCATC CTCGTCTTCG TCCGAAGACA ACAACAAAAC CAACAACATC 
AATGAAACCA ATAACAAAAT CAATCTCAAC AACAGCAGCA GCAGTAGTTC CGGTAAAACG 
GCATACCTGC CAACCAAGCT CGACGTGTTG GACAAAATGA GGGAAGGCGT CGACATGCTT 
CGGAACAATA CCAACAATTT GCTGTCCTCG GAATTGCTCA CCCAGTCAAA TCTACTCTCG 
TCCGTAAAGA TTGCACTTCC GACGCCACAA CAACCGCAGC AAACTGGAGC GACAGCTCCC 
TCAGGACCTT TCTTCGTGGC CAGCACAAGC ACTAGCAGCA CGAGCACTCT CCCTTCGGGT 
GATAAAATCG TCGGAGATGT CAAGTTTAAC AATACCATCA ACAATAATAC CGCTTCCGGT 
GGCGTAACGA AGAAAGTCCT GAACGAAGTC AAACAGCAGG AACCGGACGA TCGCGACATT 
GCCAACAATT TGAAGAACAA CACCATTGTC GTCGATTCGG TGGAAGTTGG TGCAGCAGGA 
AGTTCGAAGG AATCAACTGC TGAGTCACCT TCGGCGGCGG TCACGGTCAA TCCGATGAGT 
GTGAGTGTCC CAAATCTGAC GAGCAATGAT AACAATGCTT CACAGGAGGT ACAAACTCCA 
CCAGGGTTGT TGGAAACGTT TGCGGCCATA GCTCGAAGGC GTACTTCTGG AAGCTCTGTA 
TCCCATCATG TGAACCCTAG CTCAAACGCT TCAACCGCTT CTTCCGGAAC CAGTGGCACA 
AACAATGCTC AACCCAACAA TCAGCTGAGT AGCCTTGGGT CCAATATTCA AGCAGCGAAC 
TCTAGCTTCT TCCCGAGGGG GCAAAACTCC GTTACTAGTC TGGTGAAATT GGCCCTTTCG 
AGCAACTTCC ACAGTGGATT GTTGAGTACC GCACAAAGTT ATCCTAGTCT CTCGAGTTCG 
AGTTCGAATA ATGCAAATTC GGTCGCGGTA AGTGGAGGAA CCAACAACAA TTCAGCGAGC 
GGAGTCGGTT CCAACAATGG ATCCGTTCAA AGCGGAAATG TCCAAGCCGT GTTGAATCCA 
GCACTGACGA TGAGTCTCAC CTCAACCAGT AGCGATAGTG AGCAGGTGTC TTTGGAAGAT 
TTCCTTGAAC AGTGCCGAGC TCCAACATTG CTGGGAGATT TAGACGACGA CGAAGACATG 
GAGGACGAGA ATGATGACGA TGAAAATGAG GATGAATACG AAGAAGTCGG AAATACTTTG 
CTTCAAGTGA TGGTTTCTCG TAATTTGTTA TCGTTTATGG ATGAGGAGAC TTTGGAGAAT 
CGTCTGGCTG CCGCTGGCAA ACGCAAATCG TGGGACGACG AATTCGTGCT TAAACGCCAA 
TTCTCGGCAC TCATTCCTGC ATTCGATCCG CGTCCTGGCA GGACCAACGT CAATCAAACA 
AGTGATCTGG AAATACCTGC TCCCGGCAGT TCTACAGACA GTAGTCATCC GAGTAGCAGC 
CACTCTGAGC ATGCTCCTTT GCCTCAACCG TCCCTTGCAC TTCTTCTGCG TGGTCCCAAC 
ATAAACGGAG TAAATGATGT GGAAATTCCA CTGTCGCAGC CCGATTGGAC AATATTCCGA 
GCGGTCCAAG AACTGATCCT TCAAACCAAC ATGACCAAGC AGGATAAGTT CCGCAAAATT 
TGGCAACCAA CGTACACCAT CGTCTACAGG GAAGCCTCTT CTTCGGCCGG ACTCGGTTCC 
TGCCGCGGTG AGGACTTCAG CAGCGGAGAA GAAGGACGGG CCACCCCCGT CGTTTCCATG 
TTTTCCCAAC GCAGTGGAGG ATCTACCCTT TCGCCTAGCT CTCCGATCCC CGGAACACCA 
CTCAATCCAA CCGCCACCGC TCACTGCACT GTGGACGACG TCTTGCAGCT TCTCGGCCAA 
TTGAATGCCA TCAATCGAAC GCTTACATCT TCTCCATCCA ACAACGACAA GAACCTCATC 
CCGGACATGG AATCCAACAA TCTCAACGCG GATATATTCT TGAGCAAGAA AATTACCAAC 
AAACTGCAGC AGCAAATCCA AGACCCACTA GTCCTGGCGA GTGGAAGCCT GCCGAAATGG 
TGCGAAGACT TCAACCAAAG CTGTCCGTTC CTGTTCCCGT TCGAGACGCG GCAGCTGTAC 
TTCAACTGTA CGGCTTTCGG CGCCTCGAGG AGCATCGTGT GGCTGCAGTC GCAGCGGGAT 
GTGAACCTGG AACGGCAACG GGCTCCCGGG TTGAGCCCAC GGCATGCCGA TCAGCACGAG 
TTCCGCGTTG GGCGGTTGAA GCACGAGCGC GTCAAGGTTC CACGCGGTGA GAATCTACTC 
GAATGGGCAC AACAGGTTAT GAAAGTTCAC TGTAATCGCA AGTCGGTGTT GGAGGTGGAA 
TTCGTTGGAG AGGAAGGCAC TGGATTGGGT CCGACTTTGG AATTCTACGC CCTGGTTGCA 
GCTGAACTGC AGCGAAGTGA TCTGGGAATG TGGCTGTGCG ACGATGAACC TAAGCTAATC 
GAAGACGAAA TCGATCTAGG AGAGGGAAGC AAACCCATCG GTTATTACGT TCGTCGTTCA 
ACCGGATTGT TCCCGGCGCC GTTGCCACAG GAATCTGAAA TTTGTGACTT TGTGTCAAAC 
TACTTCTGGT TCTTGGGAGT GTTTCTGGCG AAGGTACTGC AGGACGGTCG TCTAGTCGAT 
CTGCCACTGT CGAACAGCTT CTTACAACTG TTATGTAATA ACAAATCTCT CGCTAGAGGT 
TCACTAAGCG AACTTTCTTC CAAGTCTGCC TTGCACGATG ATGTGATGAT TTCTTCTTTA 
ATGTCCGAGG AAAGCGATCG TGATCTGGTG GATTCATATC AGTCAAAATT GGCTAACGAT 
GGCTGTTGGT ATGATGGAAT CCTGTCCCAA GAAAATCTCC AAGAAATCGA TCCGATTCGG 
TACGAGTTCT TGAAAGAACT GCAAGAGTTG GTGCAGCAGA AACAAAATAT CGAACAAAAT 
GATGATCTCA CCTCCGAAGA GAAGCTACTG CAAATTAGCG AGCTTAAATT CAACACCAAA 
ACTGGCAGTG TAGCTTTGGA GGACCTCGCT CTAACGTTCA CCTATCTACC AAGTTCGAAG 
AACTACGGCT ACCAATCGGC AGATCTGATT CCAAACGGTT CCAACATTGA TGTAACGATC 
AACAACGTCG AAGAGTACTG CAATTTGACG ATCAACTTCT GCCTGCAAGA AGGCATTGCT 
AAGCAACTTA CTGCGTTCCA TCGAGGTTTC TGTGAAGTGT TCCCGCTGAA CAAATTGGCC 
GCTTTCACAT CGGAAGAAAT CCGCAAGATG CTTTGTGGCG AGCAGAACCC AGAGTGGACA 
CGGGAGGACA TTATGACCTA CACGGAACCG AAACTCGGAT ACTCCAAGGA AAGCCCTGGA 
TTCCTGCGTT TCGTGAACGT GTTGATGGGG ATGAATGCCA GCGAAAGGAA AGCATTTTTG 
CAGTTCACCA CCGGCTGCAG CAGTTTACCA CCCGGTGGGC TGGCCAATTT GCACCCGCGG 
CTGACAGTGG TGCGGAAGGT AGATGCCGGC GAAGGTTCCT ACCCTTCGGT CAACACCTGT 
GTCCACTACC TCAAATTGCC GGACTATCCG AATGAGGAAA TCTTGCGGGA ACGATTGCTG 
ACGGCCACAA AAGAAAAGGG ATTCCATCTT AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL007705 ( XM_021848324.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021704016.1 Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X3, mRNA.

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CloneID	OAe124646
Clone ID Related Accession (Same CDS sequence)	XM_021848324.1
Accession Version	XM_021848324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGATA AGCTACAGCT GGCCGTCACA GCCATCAGCA 
CCATCTCTAC TGCCCTTAAC CGTTTCCTCC TCTTCTACCA ATACTACTAG TAGCAATAGT 
ATTCTAGCCA AATATAGTTG CGCTGACCGA GTAAGCAAGT ATGTCCAGAA CCTGCGACTG 
CAACAAAACC AGCAGCAGCC ACCAGAGCGG CGTCGTAACT CTAGTTCTTA CATAATTCCA 
CCGCCGCCGC CAATGCTGCC GTTGACCTCC TCCCAGGACG ACGAAGATGG CCATCTGGTG 
GTGCTTACCA TTATCGAAGA GCTCATGTCC AAGACGCAAG ACGATTTCCT CGATCACTTT 
GCCCGGCTCG GAGTGTTCTC CAAAGTTCAG GCCCTGATGG GAGAAAACGG CTTCAGCGGG 
GGCGAAGGTG ATAACAACGA TGTGATCAAA TCCCAAGACG AGCTCAAACC GTCTGCGACC 
GGAGCACAAG ATGCTTCGGT TGTCTCCACT ACTGGAAGCT CCTCCTCTGG AACTGCCACT 
ACTAATACGG TGGAAGATGC CAAAGAAATC CTTCCCGGGA AGGCGTACCA CTGGCGAGAC 
TGGAGTATTT GTCGAGGTCG CGACTGCTTG TACGTATGGT CGGATTCAGC CGCCTTGGAG 
CTTTCGAACG GATCCAATGG GTGGTTCCGG TTCATCCTAG ACGGGAAATT GGCCACTATG 
TACTCCAGTG GTAGTCCAGA GAATGGCAGT GATAGCTCAG AAAACCGTGG TGAATTCCTA 
GAAAAATTAC AACGAGCTCG CGGAGCGGTA CGGCAGGGAA CTCCCTCCCA GCCGATTCTT 
TCGTCGCCGA GCCTTTCGCG AATCGTGGTC GGCAATTGGG TGCTGCAGAG CCAAAAAGAG 
CATCAGCTAC ATATCAACAA CTCCGAAGGT CACCAGGTGA CGATCCTGCA GGACGAACTG 
CCCGGATTCA TCTTCGAGAG CAATCGCGGC ACGAAGCATA CTTTCACGGC GGAAACCACT 
CTAGGACCGG ACTTCGCCGC CGGATGGATC AACACCAAGA AGAAGAAGAT GCGTTCGAAA 
GCGGAGGCTC AGAAATATCA GGTTAAAAAC ATCGCCCGAG ATCTGTACAA CCGGTACTTC 
AAAGCAGCTC AAGCCGTCCC GCGGGGCGCC GTGGCAAAAC TGTGCACCAT CGTGCGTCAA 
ATCGAGGCAG CCCTGGAGGA ACAATGTGCC CCTAAGGCGT CCAGTTTGCT GCAGGTTCGG 
ATTCAACCTG ACGCTTCTAA AGCAACGAAC AGCACATGGC AGGAGAAGCT GTACAACGCT 
CTGCACGATC TTGTGCAACT GTTGAACGAC GATGGAGTCA TCAGTGCTTA CGAGATGCAC 
AGTTCCGGAC TTGTACAATC CTTGGTGGCC GTGCTGTCCC GGAACTACTG GGAACTGGAG 
ATGAACCGGA GCAAGGCCAA CAAGTACCAG AAGCAACGGA TATCCATTTT CAAGAAGTGC 
ATGTATGGCG ATACCAAAAA TGGCAAAAAC ACGGCCAGCA TTCTGGTGCA GAAGTTGGTA 
TCTGTATTGG AGAGCATTGA GAAGCTTCCG GTGCACATGT ATGACTCCCC AGGGGGTAGC 
TATGGGTTGC AGATTCTCAC AAAGCGACTG AGCTTCCGAC TTGAGCGGGC AGCTTGTGAG 
CAAACTCTCT TCGATAGGAC CGGTAGAAAC CTGAAAATGG AACCATTGGC AACCGTTGGC 
CAGCTGAACA AGTATCTTCT GAAAATGGTA GCAAAGCAGT GGTACGATAT GGATCGATCG 
TCCTTCTTGT ACTTGAAAAA GCTGAAAGAG GCAAAACCAG GAAGTGTACA CTTCAAGCAC 
CAGCACGATT TCGACGAGAA TGGTCTCGTG TACTATATCG GGACGAACGG AAAAACCACG 
GAATGGGTCA ATCCTGCTCA GTACGGATTG GTTACTGTCA CAAGTAGCGA AGGCAAACAG 
CTCCCGTACG GTAAGCTGGA AGATATCCTG TCGAGAGATA GCGTTAGCGT GAATTGTCAC 
ACCAAGGATA ACAAGAAATC GTGGTTCGCC ATCGATTTGG GAATGTTTAT CATTCCAACT 
GCTTATACTT TGCGACACGC CCGTGGCTAT GGCCGCTCTG CTCTGAGAAA TTGGATGTTC 
CAGATGTCAA AGGATGGAAT AAATTGGGCG ACAATGTTGA CCCATTCGGA TGATAAAAGT 
TTGGCAGAAC CCGGAAGCAC TTGCACTTGG CCACTCGAAT GCTCTGCCGA AGAACAGCAG 
GGATGGCGTC ACGTGAGAAT CCATCAAAAT GGACGGAATG CTTCAGGGCA GACCCATTAC 
CTTAGCTTGA GTGGATTTGA AATCTACGGA AAGGTTGTCT CGGTTTGTGA TGATATGGGA 
AAAGCTGCGG CCAAGGAGAA TGAAGCTCGG CTTAGGAAAG AGCGACGACA GATTCGAGCT 
CAGTTGAAGT ATATCACTCA AGGTGCACGC GTTGTTCGAG GAGTTGATTG GCATTGGGAT 
GATCAAGATG GAAGTCCTCC TGGAGAAGGA ACCGTAACTG GTGAGATTCA TAACGGTTGG 
ATCGATGTAA AGTGGGACCA TGGTTTAAGA AACTCCTATC GCATGGGAGC CGAAGGCAAA 
TATGATCTGA AGCTGTCAAA CAGTGAAAAC CTGACTGCAC CTTACGATAT GAACAACTCC 
GGTGCTGGAC TTGTTCCAAT TAACGCAGGA AGCGTGTCTT CGGCGAAGAA AGTATATGAT 
AAGTCGTTGA ACGTTCTGAC CAGTCGCAAA TCGAGCTCTA CTCCGAGCCT CCCGGAAGCA 
ACGGAGAACA AGTCATCAGT AGCTTCGACT GAACAAGCCA CTTCCGTGGA TAACCTTGCA 
TGGAAACAAG CCGTAGAAGT GATTGCCGAA AATGTGCTAT CCTGTGCTCG ATCTGATTTG 
GCCAATACCA GTGGTGGAAG TAGCAGCAAT GACTTATCCG CTCCCGGATC CAACAATAAT 
AATCTGAACA ACCAGGAAGT GTCTGTGATT GTCCACTCGT TGGGAGAGCG GGGAAACATC 
CCAGATCTGT CGCAAATCAA TACAAGTACT TCGACTCTAG TCTCGGATTT GGCCACCATC 
ACGGAGAATC TAACACTCTC GGATAACATC AAGAACAACA TCAGCATCGG AAGTAGCACC 
CAGTTTGTGA GCAACTTTGG AACCCAACTT GCAGCTTGCT CATCCTCGTC TTCGTCCGAA 
GACAACAACA AAACCAACAA CATCAATGAA ACCAATAACA AAATCAATCT CAACAACAGC 
AGCAGCAGTA GTTCCGGTAA AACGGCATAC CTGCCAACCA AGCTCGACGT GTTGGACAAA 
ATGAGGGAAG GCGTCGACAT GCTTCGGAAC AATACCAACA ATTTGCTGTC CTCGGAATTG 
CTCACCCAGT CAAATCTACT CTCGTCCGTA AAGATTGCAC TTCCGACGCC ACAACAACCG 
CAGCAAACTG GAGCGACAGC TCCCTCAGGA CCTTTCTTCG TGGCCAGCAC AAGCACTAGC 
AGCACGAGCA CTCTCCCTTC GGGTGATAAA ATCGTCGGAG ATGTCAAGTT TAACAATACC 
ATCAACAATA ATACCGCTTC CGGTGGCGTA ACGAAGAAAG TCCTGAACGA AGTCAAACAG 
CAGGAACCGG ACGATCGCGA CATTGCCAAC AATTTGAAGA ACAACACCAT TGTCGTCGAT 
TCGGTGGAAG TTGGTGCAGC AGGAAGTTCG AAGGAATCAA CTGCTGAGTC ACCTTCGGCG 
GCGGTCACGG TCAATCCGAT GAGTGTGAGT GTCCCAAATC TGACGAGCAA TGATAACAAT 
GCTTCACAGG AGGTACAAAC TCCACCAGGG TTGTTGGAAA CGTTTGCGGC CATAGCTCGA 
AGGCGTACTT CTGGAAGCTC TGTATCCCAT CATGTGAACC CTAGCTCAAA CGCTTCAACC 
GCTTCTTCCG GAACCAGTGG CACAAACAAT GCTCAACCCA ACAATCAGCT GAGTAGCCTT 
GGGTCCAATA TTCAAGCAGC GAACTCTAGC TTCTTCCCGA GGGGGCAAAA CTCCGTTACT 
AGTCTGGTGA AATTGGCCCT TTCGAGCAAC TTCCACAGTG GATTGTTGAG TACCGCACAA 
AGTTATCCTA GTCTCTCGAG TTCGAGTTCG AATAATGCAA ATTCGGTCGC GGTAAGTGGA 
GGAACCAACA ACAATTCAGC GAGCGGAGTC GGTTCCAACA ATGGATCCGT TCAAAGCGGA 
AATGTCCAAG CCGTGTTGAA TCCAGCACTG ACGATGAGTC TCACCTCAAC CAGTAGCGAT 
AGTGAGCAGG TGTCTTTGGA AGATTTCCTT GAACAGTGCC GAGCTCCAAC ATTGCTGGGA 
GATTTAGACG ACGACGAAGA CATGGAGGAC GAGAATGATG ACGATGAAAA TGAGGATGAA 
TACGAAGAAG TCGGAAATAC TTTGCTTCAA GTGATGGTTT CTCGTAATTT GTTATCGTTT 
ATGGATGAGG AGACTTTGGA GAATCGTCTG GCTGCCGCTG GCAAACGCAA ATCGTGGGAC 
GACGAATTCG TGCTTAAACG CCAATTCTCG GCACTCATTC CTGCATTCGA TCCGCGTCCT 
GGCAGGACCA ACGTCAATCA AACAAGTGAT CTGGAAATAC CTGCTCCCGG CAGTTCTACA 
GACAGTAGTC ATCCGAGTAG CAGCCACTCT GAGCATGCTC CTTTGCCTCA ACCGTCCCTT 
GCACTTCTTC TGCGTGGTCC CAACATAAAC GGAGTAAATG ATGTGGAAAT TCCACTGTCG 
CAGCCCGATT GGACAATATT CCGAGCGGTC CAAGAACTGA TCCTTCAAAC CAACATGACC 
AAGCAGGATA AGTTCCGCAA AATTTGGCAA CCAACGTACA CCATCGTCTA CAGGGAAGCC 
TCTTCTTCGG CCGGACTCGG TTCCTGCCGC GGTGAGGACT TCAGCAGCGG AGAAGAAGGA 
CGGGCCACCC CCGTCGTTTC CATGTTTTCC CAACGCAGTG GAGGATCTAC CCTTTCGCCT 
AGCTCTCCGA TCCCCGGAAC ACCACTCAAT CCAACCGCCA CCGCTCACTG CACTGTGGAC 
GACGTCTTGC AGCTTCTCGG CCAATTGAAT GCCATCAATC GAACGCTTAC ATCTTCTCCA 
TCCAACAACG ACAAGAACCT CATCCCGGAC ATGGAATCCA ACAATCTCAA CGCGGATATA 
TTCTTGAGCA AGAAAATTAC CAACAAACTG CAGCAGCAAA TCCAAGACCC ACTAGTCCTG 
GCGAGTGGAA GCCTGCCGAA ATGGTGCGAA GACTTCAACC AAAGCTGTCC GTTCCTGTTC 
CCGTTCGAGA CGCGGCAGCT GTACTTCAAC TGTACGGCTT TCGGCGCCTC GAGGAGCATC 
GTGTGGCTGC AGTCGCAGCG GGATGTGAAC CTGGAACGGC AACGGGCTCC CGGGTTGAGC 
CCACGGCATG CCGATCAGCA CGAGTTCCGC GTTGGGCGGT TGAAGCACGA GCGCGTCAAG 
GTTCCACGCG GTGAGAATCT ACTCGAATGG GCACAACAGG TTATGAAAGT TCACTGTAAT 
CGCAAGTCGG TGTTGGAGGT GGAATTCGTT GGAGAGGAAG GCACTGGATT GGGTCCGACT 
TTGGAATTCT ACGCCCTGGT TGCAGCTGAA CTGCAGCGAA GTGATCTGGG AATGTGGCTG 
TGCGACGATG AACCTAAGCT AATCGAAGAC GAAATCGATC TAGGAGAGGG AAGCAAACCC 
ATCGGTTATT ACGTTCGTCG TTCAACCGGA TTGTTCCCGG CGCCGTTGCC ACAGGAATCT 
GAAATTTGTG ACTTTGTGTC AAACTACTTC TGGTTCTTGG GAGTGTTTCT GGCGAAGGTA 
CTGCAGGACG GTCGTCTAGT CGATCTGCCA CTGTCGAACA GCTTCTTACA ACTGTTATGT 
AATAACAAAT CTCTCGCTAG AGGTTCACTA AGCGAACTTT CTTCCAAGTC TGCCTTGCAC 
GATGATGTGA TGATTTCTTC TTTAATGTCC GAGGAAAGCG ATCGTGATCT GGTGGATTCA 
TATCAGTCAA AATTGGCTAA CGATGGCTGT TGGTATGATG GAATCCTGTC CCAAGAAAAT 
CTCCAAGAAA TCGATCCGAT TCGGTACGAG TTCTTGAAAG AACTGCAAGA GTTGGTGCAG 
CAGAAACAAA ATATCGAACA AAATGATGAT CTCACCTCCG AAGAGAAGCT ACTGCAAATT 
AGCGAGCTTA AATTCAACAC CAAAACTGGC AGTGTAGCTT TGGAGGACCT CGCTCTAACG 
TTCACCTATC TACCAAGTTC GAAGAACTAC GGCTACCAAT CGGCAGATCT GATTCCAAAC 
GGTTCCAACA TTGATGTAAC GATCAACAAC GTCGAAGAGT ACTGCAATTT GACGATCAAC 
TTCTGCCTGC AAGAAGGCAT TGCTAAGCAA CTTACTGCGT TCCATCGAGG TTTCTGTGAA 
GTGTTCCCGC TGAACAAATT GGCCGCTTTC ACATCGGAAG AAATCCGCAA GATGCTTTGT 
GGCGAGCAGA ACCCAGAGTG GACACGGGAG GACATTATGA CCTACACGGA ACCGAAACTC 
GGATACTCCA AGGAAAGCCC TGGATTCCTG CGTTTCGTGA ACGTGTTGAT GGGGATGAAT 
GCCAGCGAAA GGAAAGCATT TTTGCAGTTC ACCACCGGCT GCAGCAGTTT ACCACCCGGT 
GGGCTGGCCA ATTTGCACCC GCGGCTGACA GTGGTGCGGA AGGTAGATGC CGGCGAAGGT 
TCCTACCCTT CGGTCAACAC CTGTGTCCAC TACCTCAAAT TGCCGGACTA TCCGAATGAG 
GAAATCTTGC GGGAACGATT GCTGACGGCC ACAAAAGAAA AGGGATTCCA TCTTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021704016.1
CDS	934..9633
Translation	MGDVDPETLLEWLSMGQGDERDMQLIALEQLCMLLLMSDNVDRCFESCPPRTFLPALCKIFLDELAPENVLEVTARAITYYLDVSSECTRRIVAIDGAIKAICNRLVVADLESRTSRDLAEQCIKVLELICTREAGAVFEGGGLNCVLSFIRDNGSQIHKDTLHSAMAVVSRLCTKVEPQGANVQTCVESLSTLLQHEDPLVADGALKCFASVADRFTRKGVDPAPLAEYGLVTELLNRLSNAAGPQTSALTAGASSNATPAAQVNSQETTGTVQLSSSAPKTQGAAEAGRSSQSIATTISLLSTLCRGSPSITHDLLRSNLPDAMERAFKGDERCVLDCMRLADLILLLLFEGRQALGRVGGSSGQLAPRVRRADSSTERTHRQLIDCIRSKDTEALIEAIESGGIDVNCMDDVGQTLLNWASAFGTLEMVEFLCDKGADVNKGQRSSSLHYAACFGRPGIAKVLLKHGANPDLRDEDGKTPLDKARERPDEGHREVASILQSPGEWMTAATRSDLKTDPEDGESEPRGDPEMAPVYLKFFLPIFCKTFQSTMLASVRRSSLGLIKKMIQYVQPEMLSRLCSSEGLQSHEHSLGTLLVEVVASVLDNEISYSWPSQPSAPSLLPLTVSSSSTNTTSSNSILAKYSCADRVSKYVQNLRLQQNQQQPPERRRNSSSYIIPPPPPMLPLTSSQDDEDGHLVVLTIIEELMSKTQDDFLDHFARLGVFSKVQALMGENGFSGGEGDNNDVIKSQDELKPSATGAQDASVVSTTGSSSSGTATTNTVEDAKEILPGKAYHWRDWSICRGRDCLYVWSDSAALELSNGSNGWFRFILDGKLATMYSSGSPENGSDSSENRGEFLEKLQRARGAVRQGTPSQPILSSPSLSRIVVGNWVLQSQKEHQLHINNSEGHQVTILQDELPGFIFESNRGTKHTFTAETTLGPDFAAGWINTKKKKMRSKAEAQKYQVKNIARDLYNRYFKAAQAVPRGAVAKLCTIVRQIEAALEEQCAPKASSLLQVRIQPDASKATNSTWQEKLYNALHDLVQLLNDDGVISAYEMHSSGLVQSLVAVLSRNYWELEMNRSKANKYQKQRISIFKKCMYGDTKNGKNTASILVQKLVSVLESIEKLPVHMYDSPGGSYGLQILTKRLSFRLERAACEQTLFDRTGRNLKMEPLATVGQLNKYLLKMVAKQWYDMDRSSFLYLKKLKEAKPGSVHFKHQHDFDENGLVYYIGTNGKTTEWVNPAQYGLVTVTSSEGKQLPYGKLEDILSRDSVSVNCHTKDNKKSWFAIDLGMFIIPTAYTLRHARGYGRSALRNWMFQMSKDGINWATMLTHSDDKSLAEPGSTCTWPLECSAEEQQGWRHVRIHQNGRNASGQTHYLSLSGFEIYGKVVSVCDDMGKAAAKENEARLRKERRQIRAQLKYITQGARVVRGVDWHWDDQDGSPPGEGTVTGEIHNGWIDVKWDHGLRNSYRMGAEGKYDLKLSNSENLTAPYDMNNSGAGLVPINAGSVSSAKKVYDKSLNVLTSRKSSSTPSLPEATENKSSVASTEQATSVDNLAWKQAVEVIAENVLSCARSDLANTSGGSSSNDLSAPGSNNNNLNNQEVSVIVHSLGERGNIPDLSQINTSTSTLVSDLATITENLTLSDNIKNNISIGSSTQFVSNFGTQLAACSSSSSSEDNNKTNNINETNNKINLNNSSSSSSGKTAYLPTKLDVLDKMREGVDMLRNNTNNLLSSELLTQSNLLSSVKIALPTPQQPQQTGATAPSGPFFVASTSTSSTSTLPSGDKIVGDVKFNNTINNNTASGGVTKKVLNEVKQQEPDDRDIANNLKNNTIVVDSVEVGAAGSSKESTAESPSAAVTVNPMSVSVPNLTSNDNNASQEVQTPPGLLETFAAIARRRTSGSSVSHHVNPSSNASTASSGTSGTNNAQPNNQLSSLGSNIQAANSSFFPRGQNSVTSLVKLALSSNFHSGLLSTAQSYPSLSSSSSNNANSVAVSGGTNNNSASGVGSNNGSVQSGNVQAVLNPALTMSLTSTSSDSEQVSLEDFLEQCRAPTLLGDLDDDEDMEDENDDDENEDEYEEVGNTLLQVMVSRNLLSFMDEETLENRLAAAGKRKSWDDEFVLKRQFSALIPAFDPRPGRTNVNQTSDLEIPAPGSSTDSSHPSSSHSEHAPLPQPSLALLLRGPNINGVNDVEIPLSQPDWTIFRAVQELILQTNMTKQDKFRKIWQPTYTIVYREASSSAGLGSCRGEDFSSGEEGRATPVVSMFSQRSGGSTLSPSSPIPGTPLNPTATAHCTVDDVLQLLGQLNAINRTLTSSPSNNDKNLIPDMESNNLNADIFLSKKITNKLQQQIQDPLVLASGSLPKWCEDFNQSCPFLFPFETRQLYFNCTAFGASRSIVWLQSQRDVNLERQRAPGLSPRHADQHEFRVGRLKHERVKVPRGENLLEWAQQVMKVHCNRKSVLEVEFVGEEGTGLGPTLEFYALVAAELQRSDLGMWLCDDEPKLIEDEIDLGEGSKPIGYYVRRSTGLFPAPLPQESEICDFVSNYFWFLGVFLAKVLQDGRLVDLPLSNSFLQLLCNNKSLARGSLSELSSKSALHDDVMISSLMSEESDRDLVDSYQSKLANDGCWYDGILSQENLQEIDPIRYEFLKELQELVQQKQNIEQNDDLTSEEKLLQISELKFNTKTGSVALEDLALTFTYLPSSKNYGYQSADLIPNGSNIDVTINNVEEYCNLTINFCLQEGIAKQLTAFHRGFCEVFPLNKLAAFTSEEIRKMLCGEQNPEWTREDIMTYTEPKLGYSKESPGFLRFVNVLMGMNASERKAFLQFTTGCSSLPPGGLANLHPRLTVVRKVDAGEGSYPSVNTCVHYLKLPDYPNEEILRERLLTATKEKGFHLN

Target ORF information:

RefSeq Version	XM_021848324.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848324.1

ORF Insert Sequence:

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGATA AGCTACAGCT GGCCGTCACA GCCATCAGCA 
CCATCTCTAC TGCCCTTAAC CGTTTCCTCC TCTTCTACCA ATACTACTAG TAGCAATAGT 
ATTCTAGCCA AATATAGTTG CGCTGACCGA GTAAGCAAGT ATGTCCAGAA CCTGCGACTG 
CAACAAAACC AGCAGCAGCC ACCAGAGCGG CGTCGTAACT CTAGTTCTTA CATAATTCCA 
CCGCCGCCGC CAATGCTGCC GTTGACCTCC TCCCAGGACG ACGAAGATGG CCATCTGGTG 
GTGCTTACCA TTATCGAAGA GCTCATGTCC AAGACGCAAG ACGATTTCCT CGATCACTTT 
GCCCGGCTCG GAGTGTTCTC CAAAGTTCAG GCCCTGATGG GAGAAAACGG CTTCAGCGGG 
GGCGAAGGTG ATAACAACGA TGTGATCAAA TCCCAAGACG AGCTCAAACC GTCTGCGACC 
GGAGCACAAG ATGCTTCGGT TGTCTCCACT ACTGGAAGCT CCTCCTCTGG AACTGCCACT 
ACTAATACGG TGGAAGATGC CAAAGAAATC CTTCCCGGGA AGGCGTACCA CTGGCGAGAC 
TGGAGTATTT GTCGAGGTCG CGACTGCTTG TACGTATGGT CGGATTCAGC CGCCTTGGAG 
CTTTCGAACG GATCCAATGG GTGGTTCCGG TTCATCCTAG ACGGGAAATT GGCCACTATG 
TACTCCAGTG GTAGTCCAGA GAATGGCAGT GATAGCTCAG AAAACCGTGG TGAATTCCTA 
GAAAAATTAC AACGAGCTCG CGGAGCGGTA CGGCAGGGAA CTCCCTCCCA GCCGATTCTT 
TCGTCGCCGA GCCTTTCGCG AATCGTGGTC GGCAATTGGG TGCTGCAGAG CCAAAAAGAG 
CATCAGCTAC ATATCAACAA CTCCGAAGGT CACCAGGTGA CGATCCTGCA GGACGAACTG 
CCCGGATTCA TCTTCGAGAG CAATCGCGGC ACGAAGCATA CTTTCACGGC GGAAACCACT 
CTAGGACCGG ACTTCGCCGC CGGATGGATC AACACCAAGA AGAAGAAGAT GCGTTCGAAA 
GCGGAGGCTC AGAAATATCA GGTTAAAAAC ATCGCCCGAG ATCTGTACAA CCGGTACTTC 
AAAGCAGCTC AAGCCGTCCC GCGGGGCGCC GTGGCAAAAC TGTGCACCAT CGTGCGTCAA 
ATCGAGGCAG CCCTGGAGGA ACAATGTGCC CCTAAGGCGT CCAGTTTGCT GCAGGTTCGG 
ATTCAACCTG ACGCTTCTAA AGCAACGAAC AGCACATGGC AGGAGAAGCT GTACAACGCT 
CTGCACGATC TTGTGCAACT GTTGAACGAC GATGGAGTCA TCAGTGCTTA CGAGATGCAC 
AGTTCCGGAC TTGTACAATC CTTGGTGGCC GTGCTGTCCC GGAACTACTG GGAACTGGAG 
ATGAACCGGA GCAAGGCCAA CAAGTACCAG AAGCAACGGA TATCCATTTT CAAGAAGTGC 
ATGTATGGCG ATACCAAAAA TGGCAAAAAC ACGGCCAGCA TTCTGGTGCA GAAGTTGGTA 
TCTGTATTGG AGAGCATTGA GAAGCTTCCG GTGCACATGT ATGACTCCCC AGGGGGTAGC 
TATGGGTTGC AGATTCTCAC AAAGCGACTG AGCTTCCGAC TTGAGCGGGC AGCTTGTGAG 
CAAACTCTCT TCGATAGGAC CGGTAGAAAC CTGAAAATGG AACCATTGGC AACCGTTGGC 
CAGCTGAACA AGTATCTTCT GAAAATGGTA GCAAAGCAGT GGTACGATAT GGATCGATCG 
TCCTTCTTGT ACTTGAAAAA GCTGAAAGAG GCAAAACCAG GAAGTGTACA CTTCAAGCAC 
CAGCACGATT TCGACGAGAA TGGTCTCGTG TACTATATCG GGACGAACGG AAAAACCACG 
GAATGGGTCA ATCCTGCTCA GTACGGATTG GTTACTGTCA CAAGTAGCGA AGGCAAACAG 
CTCCCGTACG GTAAGCTGGA AGATATCCTG TCGAGAGATA GCGTTAGCGT GAATTGTCAC 
ACCAAGGATA ACAAGAAATC GTGGTTCGCC ATCGATTTGG GAATGTTTAT CATTCCAACT 
GCTTATACTT TGCGACACGC CCGTGGCTAT GGCCGCTCTG CTCTGAGAAA TTGGATGTTC 
CAGATGTCAA AGGATGGAAT AAATTGGGCG ACAATGTTGA CCCATTCGGA TGATAAAAGT 
TTGGCAGAAC CCGGAAGCAC TTGCACTTGG CCACTCGAAT GCTCTGCCGA AGAACAGCAG 
GGATGGCGTC ACGTGAGAAT CCATCAAAAT GGACGGAATG CTTCAGGGCA GACCCATTAC 
CTTAGCTTGA GTGGATTTGA AATCTACGGA AAGGTTGTCT CGGTTTGTGA TGATATGGGA 
AAAGCTGCGG CCAAGGAGAA TGAAGCTCGG CTTAGGAAAG AGCGACGACA GATTCGAGCT 
CAGTTGAAGT ATATCACTCA AGGTGCACGC GTTGTTCGAG GAGTTGATTG GCATTGGGAT 
GATCAAGATG GAAGTCCTCC TGGAGAAGGA ACCGTAACTG GTGAGATTCA TAACGGTTGG 
ATCGATGTAA AGTGGGACCA TGGTTTAAGA AACTCCTATC GCATGGGAGC CGAAGGCAAA 
TATGATCTGA AGCTGTCAAA CAGTGAAAAC CTGACTGCAC CTTACGATAT GAACAACTCC 
GGTGCTGGAC TTGTTCCAAT TAACGCAGGA AGCGTGTCTT CGGCGAAGAA AGTATATGAT 
AAGTCGTTGA ACGTTCTGAC CAGTCGCAAA TCGAGCTCTA CTCCGAGCCT CCCGGAAGCA 
ACGGAGAACA AGTCATCAGT AGCTTCGACT GAACAAGCCA CTTCCGTGGA TAACCTTGCA 
TGGAAACAAG CCGTAGAAGT GATTGCCGAA AATGTGCTAT CCTGTGCTCG ATCTGATTTG 
GCCAATACCA GTGGTGGAAG TAGCAGCAAT GACTTATCCG CTCCCGGATC CAACAATAAT 
AATCTGAACA ACCAGGAAGT GTCTGTGATT GTCCACTCGT TGGGAGAGCG GGGAAACATC 
CCAGATCTGT CGCAAATCAA TACAAGTACT TCGACTCTAG TCTCGGATTT GGCCACCATC 
ACGGAGAATC TAACACTCTC GGATAACATC AAGAACAACA TCAGCATCGG AAGTAGCACC 
CAGTTTGTGA GCAACTTTGG AACCCAACTT GCAGCTTGCT CATCCTCGTC TTCGTCCGAA 
GACAACAACA AAACCAACAA CATCAATGAA ACCAATAACA AAATCAATCT CAACAACAGC 
AGCAGCAGTA GTTCCGGTAA AACGGCATAC CTGCCAACCA AGCTCGACGT GTTGGACAAA 
ATGAGGGAAG GCGTCGACAT GCTTCGGAAC AATACCAACA ATTTGCTGTC CTCGGAATTG 
CTCACCCAGT CAAATCTACT CTCGTCCGTA AAGATTGCAC TTCCGACGCC ACAACAACCG 
CAGCAAACTG GAGCGACAGC TCCCTCAGGA CCTTTCTTCG TGGCCAGCAC AAGCACTAGC 
AGCACGAGCA CTCTCCCTTC GGGTGATAAA ATCGTCGGAG ATGTCAAGTT TAACAATACC 
ATCAACAATA ATACCGCTTC CGGTGGCGTA ACGAAGAAAG TCCTGAACGA AGTCAAACAG 
CAGGAACCGG ACGATCGCGA CATTGCCAAC AATTTGAAGA ACAACACCAT TGTCGTCGAT 
TCGGTGGAAG TTGGTGCAGC AGGAAGTTCG AAGGAATCAA CTGCTGAGTC ACCTTCGGCG 
GCGGTCACGG TCAATCCGAT GAGTGTGAGT GTCCCAAATC TGACGAGCAA TGATAACAAT 
GCTTCACAGG AGGTACAAAC TCCACCAGGG TTGTTGGAAA CGTTTGCGGC CATAGCTCGA 
AGGCGTACTT CTGGAAGCTC TGTATCCCAT CATGTGAACC CTAGCTCAAA CGCTTCAACC 
GCTTCTTCCG GAACCAGTGG CACAAACAAT GCTCAACCCA ACAATCAGCT GAGTAGCCTT 
GGGTCCAATA TTCAAGCAGC GAACTCTAGC TTCTTCCCGA GGGGGCAAAA CTCCGTTACT 
AGTCTGGTGA AATTGGCCCT TTCGAGCAAC TTCCACAGTG GATTGTTGAG TACCGCACAA 
AGTTATCCTA GTCTCTCGAG TTCGAGTTCG AATAATGCAA ATTCGGTCGC GGTAAGTGGA 
GGAACCAACA ACAATTCAGC GAGCGGAGTC GGTTCCAACA ATGGATCCGT TCAAAGCGGA 
AATGTCCAAG CCGTGTTGAA TCCAGCACTG ACGATGAGTC TCACCTCAAC CAGTAGCGAT 
AGTGAGCAGG TGTCTTTGGA AGATTTCCTT GAACAGTGCC GAGCTCCAAC ATTGCTGGGA 
GATTTAGACG ACGACGAAGA CATGGAGGAC GAGAATGATG ACGATGAAAA TGAGGATGAA 
TACGAAGAAG TCGGAAATAC TTTGCTTCAA GTGATGGTTT CTCGTAATTT GTTATCGTTT 
ATGGATGAGG AGACTTTGGA GAATCGTCTG GCTGCCGCTG GCAAACGCAA ATCGTGGGAC 
GACGAATTCG TGCTTAAACG CCAATTCTCG GCACTCATTC CTGCATTCGA TCCGCGTCCT 
GGCAGGACCA ACGTCAATCA AACAAGTGAT CTGGAAATAC CTGCTCCCGG CAGTTCTACA 
GACAGTAGTC ATCCGAGTAG CAGCCACTCT GAGCATGCTC CTTTGCCTCA ACCGTCCCTT 
GCACTTCTTC TGCGTGGTCC CAACATAAAC GGAGTAAATG ATGTGGAAAT TCCACTGTCG 
CAGCCCGATT GGACAATATT CCGAGCGGTC CAAGAACTGA TCCTTCAAAC CAACATGACC 
AAGCAGGATA AGTTCCGCAA AATTTGGCAA CCAACGTACA CCATCGTCTA CAGGGAAGCC 
TCTTCTTCGG CCGGACTCGG TTCCTGCCGC GGTGAGGACT TCAGCAGCGG AGAAGAAGGA 
CGGGCCACCC CCGTCGTTTC CATGTTTTCC CAACGCAGTG GAGGATCTAC CCTTTCGCCT 
AGCTCTCCGA TCCCCGGAAC ACCACTCAAT CCAACCGCCA CCGCTCACTG CACTGTGGAC 
GACGTCTTGC AGCTTCTCGG CCAATTGAAT GCCATCAATC GAACGCTTAC ATCTTCTCCA 
TCCAACAACG ACAAGAACCT CATCCCGGAC ATGGAATCCA ACAATCTCAA CGCGGATATA 
TTCTTGAGCA AGAAAATTAC CAACAAACTG CAGCAGCAAA TCCAAGACCC ACTAGTCCTG 
GCGAGTGGAA GCCTGCCGAA ATGGTGCGAA GACTTCAACC AAAGCTGTCC GTTCCTGTTC 
CCGTTCGAGA CGCGGCAGCT GTACTTCAAC TGTACGGCTT TCGGCGCCTC GAGGAGCATC 
GTGTGGCTGC AGTCGCAGCG GGATGTGAAC CTGGAACGGC AACGGGCTCC CGGGTTGAGC 
CCACGGCATG CCGATCAGCA CGAGTTCCGC GTTGGGCGGT TGAAGCACGA GCGCGTCAAG 
GTTCCACGCG GTGAGAATCT ACTCGAATGG GCACAACAGG TTATGAAAGT TCACTGTAAT 
CGCAAGTCGG TGTTGGAGGT GGAATTCGTT GGAGAGGAAG GCACTGGATT GGGTCCGACT 
TTGGAATTCT ACGCCCTGGT TGCAGCTGAA CTGCAGCGAA GTGATCTGGG AATGTGGCTG 
TGCGACGATG AACCTAAGCT AATCGAAGAC GAAATCGATC TAGGAGAGGG AAGCAAACCC 
ATCGGTTATT ACGTTCGTCG TTCAACCGGA TTGTTCCCGG CGCCGTTGCC ACAGGAATCT 
GAAATTTGTG ACTTTGTGTC AAACTACTTC TGGTTCTTGG GAGTGTTTCT GGCGAAGGTA 
CTGCAGGACG GTCGTCTAGT CGATCTGCCA CTGTCGAACA GCTTCTTACA ACTGTTATGT 
AATAACAAAT CTCTCGCTAG AGGTTCACTA AGCGAACTTT CTTCCAAGTC TGCCTTGCAC 
GATGATGTGA TGATTTCTTC TTTAATGTCC GAGGAAAGCG ATCGTGATCT GGTGGATTCA 
TATCAGTCAA AATTGGCTAA CGATGGCTGT TGGTATGATG GAATCCTGTC CCAAGAAAAT 
CTCCAAGAAA TCGATCCGAT TCGGTACGAG TTCTTGAAAG AACTGCAAGA GTTGGTGCAG 
CAGAAACAAA ATATCGAACA AAATGATGAT CTCACCTCCG AAGAGAAGCT ACTGCAAATT 
AGCGAGCTTA AATTCAACAC CAAAACTGGC AGTGTAGCTT TGGAGGACCT CGCTCTAACG 
TTCACCTATC TACCAAGTTC GAAGAACTAC GGCTACCAAT CGGCAGATCT GATTCCAAAC 
GGTTCCAACA TTGATGTAAC GATCAACAAC GTCGAAGAGT ACTGCAATTT GACGATCAAC 
TTCTGCCTGC AAGAAGGCAT TGCTAAGCAA CTTACTGCGT TCCATCGAGG TTTCTGTGAA 
GTGTTCCCGC TGAACAAATT GGCCGCTTTC ACATCGGAAG AAATCCGCAA GATGCTTTGT 
GGCGAGCAGA ACCCAGAGTG GACACGGGAG GACATTATGA CCTACACGGA ACCGAAACTC 
GGATACTCCA AGGAAAGCCC TGGATTCCTG CGTTTCGTGA ACGTGTTGAT GGGGATGAAT 
GCCAGCGAAA GGAAAGCATT TTTGCAGTTC ACCACCGGCT GCAGCAGTTT ACCACCCGGT 
GGGCTGGCCA ATTTGCACCC GCGGCTGACA GTGGTGCGGA AGGTAGATGC CGGCGAAGGT 
TCCTACCCTT CGGTCAACAC CTGTGTCCAC TACCTCAAAT TGCCGGACTA TCCGAATGAG 
GAAATCTTGC GGGAACGATT GCTGACGGCC ACAAAAGAAA AGGGATTCCA TCTTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL007705 ( XM_021848325.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021704017.1 Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X4, mRNA.

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CloneID	OAe124647
Clone ID Related Accession (Same CDS sequence)	XM_021848325.1
Accession Version	XM_021848325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGGAC GACGAAGATG GCCATCTGGT GGTGCTTACC 
ATTATCGAAG AGCTCATGTC CAAGACGCAA GACGATTTCC TCGATCACTT TGCCCGGCTC 
GGAGTGTTCT CCAAAGTTCA GGCCCTGATG GGAGAAAACG GCTTCAGCGG GGGCGAAGGT 
GATAACAACG ATGTGATCAA ATCCCAAGAC GAGCTCAAAC CGTCTGCGAC CGGAGCACAA 
GATGCTTCGG TTGTCTCCAC TACTGGAAGC TCCTCCTCTG GAACTGCCAC TACTAATACG 
GTGGAAGATG CCAAAGAAAT CCTTCCCGGG AAGGCGTACC ACTGGCGAGA CTGGAGTATT 
TGTCGAGGTC GCGACTGCTT GTACGTATGG TCGGATTCAG CCGCCTTGGA GCTTTCGAAC 
GGATCCAATG GGTGGTTCCG GTTCATCCTA GACGGGAAAT TGGCCACTAT GTACTCCAGT 
GGTAGTCCAG AGAATGGCAG TGATAGCTCA GGCAAGGGAA GGACAGCGGA TTCCTTAGCC 
GGCGAAGAAA ACCGTGGTGA ATTCCTAGAA AAATTACAAC GAGCTCGCGG AGCGGTACGG 
CAGGGAACTC CCTCCCAGCC GATTCTTTCG TCGCCGAGCC TTTCGCGAAT CGTGGTCGGC 
AATTGGGTGC TGCAGAGCCA AAAAGAGCAT CAGCTACATA TCAACAACTC CGAAGGTCAC 
CAGGTGACGA TCCTGCAGGA CGAACTGCCC GGATTCATCT TCGAGAGCAA TCGCGGCACG 
AAGCATACTT TCACGGCGGA AACCACTCTA GGACCGGACT TCGCCGCCGG ATGGATCAAC 
ACCAAGAAGA AGAAGATGCG TTCGAAAGCG GAGGCTCAGA AATATCAGGT TAAAAACATC 
GCCCGAGATC TGTACAACCG GTACTTCAAA GCAGCTCAAG CCGTCCCGCG GGGCGCCGTG 
GCAAAACTGT GCACCATCGT GCGTCAAATC GAGGCAGCCC TGGAGGAACA ATGTGCCCCT 
AAGGCGTCCA GTTTGCTGCA GGTTCGGATT CAACCTGACG CTTCTAAAGC AACGAACAGC 
ACATGGCAGG AGAAGCTGTA CAACGCTCTG CACGATCTTG TGCAACTGTT GAACGACGAT 
GGAGTCATCA GTGCTTACGA GATGCACAGT TCCGGACTTG TACAATCCTT GGTGGCCGTG 
CTGTCCCGGA ACTACTGGGA ACTGGAGATG AACCGGAGCA AGGCCAACAA GTACCAGAAG 
CAACGGATAT CCATTTTCAA GAAGTGCATG TATGGCGATA CCAAAAATGG CAAAAACACG 
GCCAGCATTC TGGTGCAGAA GTTGGTATCT GTATTGGAGA GCATTGAGAA GCTTCCGGTG 
CACATGTATG ACTCCCCAGG GGGTAGCTAT GGGTTGCAGA TTCTCACAAA GCGACTGAGC 
TTCCGACTTG AGCGGGCAGC TTGTGAGCAA ACTCTCTTCG ATAGGACCGG TAGAAACCTG 
AAAATGGAAC CATTGGCAAC CGTTGGCCAG CTGAACAAGT ATCTTCTGAA AATGGTAGCA 
AAGCAGTGGT ACGATATGGA TCGATCGTCC TTCTTGTACT TGAAAAAGCT GAAAGAGGCA 
AAACCAGGAA GTGTACACTT CAAGCACCAG CACGATTTCG ACGAGAATGG TCTCGTGTAC 
TATATCGGGA CGAACGGAAA AACCACGGAA TGGGTCAATC CTGCTCAGTA CGGATTGGTT 
ACTGTCACAA GTAGCGAAGG CAAACAGCTC CCGTACGGTA AGCTGGAAGA TATCCTGTCG 
AGAGATAGCG TTAGCGTGAA TTGTCACACC AAGGATAACA AGAAATCGTG GTTCGCCATC 
GATTTGGGAA TGTTTATCAT TCCAACTGCT TATACTTTGC GACACGCCCG TGGCTATGGC 
CGCTCTGCTC TGAGAAATTG GATGTTCCAG ATGTCAAAGG ATGGAATAAA TTGGGCGACA 
ATGTTGACCC ATTCGGATGA TAAAAGTTTG GCAGAACCCG GAAGCACTTG CACTTGGCCA 
CTCGAATGCT CTGCCGAAGA ACAGCAGGGA TGGCGTCACG TGAGAATCCA TCAAAATGGA 
CGGAATGCTT CAGGGCAGAC CCATTACCTT AGCTTGAGTG GATTTGAAAT CTACGGAAAG 
GTTGTCTCGG TTTGTGATGA TATGGGAAAA GCTGCGGCCA AGGAGAATGA AGCTCGGCTT 
AGGAAAGAGC GACGACAGAT TCGAGCTCAG TTGAAGTATA TCACTCAAGG TGCACGCGTT 
GTTCGAGGAG TTGATTGGCA TTGGGATGAT CAAGATGGAA GTCCTCCTGG AGAAGGAACC 
GTAACTGGTG AGATTCATAA CGGTTGGATC GATGTAAAGT GGGACCATGG TTTAAGAAAC 
TCCTATCGCA TGGGAGCCGA AGGCAAATAT GATCTGAAGC TGTCAAACAG TGAAAACCTG 
ACTGCACCTT ACGATATGAA CAACTCCGGT GCTGGACTTG TTCCAATTAA CGCAGGAAGC 
GTGTCTTCGG CGAAGAAAGT ATATGATAAG TCGTTGAACG TTCTGACCAG TCGCAAATCG 
AGCTCTACTC CGAGCCTCCC GGAAGCAACG GAGAACAAGT CATCAGTAGC TTCGACTGAA 
CAAGCCACTT CCGTGGATAA CCTTGCATGG AAACAAGCCG TAGAAGTGAT TGCCGAAAAT 
GTGCTATCCT GTGCTCGATC TGATTTGGCC AATACCAGTG GTGGAAGTAG CAGCAATGAC 
TTATCCGCTC CCGGATCCAA CAATAATAAT CTGAACAACC AGGAAGTGTC TGTGATTGTC 
CACTCGTTGG GAGAGCGGGG AAACATCCCA GATCTGTCGC AAATCAATAC AAGTACTTCG 
ACTCTAGTCT CGGATTTGGC CACCATCACG GAGAATCTAA CACTCTCGGA TAACATCAAG 
AACAACATCA GCATCGGAAG TAGCACCCAG TTTGTGAGCA ACTTTGGAAC CCAACTTGCA 
GCTTGCTCAT CCTCGTCTTC GTCCGAAGAC AACAACAAAA CCAACAACAT CAATGAAACC 
AATAACAAAA TCAATCTCAA CAACAGCAGC AGCAGTAGTT CCGGTAAAAC GGCATACCTG 
CCAACCAAGC TCGACGTGTT GGACAAAATG AGGGAAGGCG TCGACATGCT TCGGAACAAT 
ACCAACAATT TGCTGTCCTC GGAATTGCTC ACCCAGTCAA ATCTACTCTC GTCCGTAAAG 
ATTGCACTTC CGACGCCACA ACAACCGCAG CAAACTGGAG CGACAGCTCC CTCAGGACCT 
TTCTTCGTGG CCAGCACAAG CACTAGCAGC ACGAGCACTC TCCCTTCGGG TGATAAAATC 
GTCGGAGATG TCAAGTTTAA CAATACCATC AACAATAATA CCGCTTCCGG TGGCGTAACG 
AAGAAAGTCC TGAACGAAGT CAAACAGCAG GAACCGGACG ATCGCGACAT TGCCAACAAT 
TTGAAGAACA ACACCATTGT CGTCGATTCG GTGGAAGTTG GTGCAGCAGG AAGTTCGAAG 
GAATCAACTG CTGAGTCACC TTCGGCGGCG GTCACGGTCA ATCCGATGAG TGTGAGTGTC 
CCAAATCTGA CGAGCAATGA TAACAATGCT TCACAGGAGG TACAAACTCC ACCAGGGTTG 
TTGGAAACGT TTGCGGCCAT AGCTCGAAGG CGTACTTCTG GAAGCTCTGT ATCCCATCAT 
GTGAACCCTA GCTCAAACGC TTCAACCGCT TCTTCCGGAA CCAGTGGCAC AAACAATGCT 
CAACCCAACA ATCAGCTGAG TAGCCTTGGG TCCAATATTC AAGCAGCGAA CTCTAGCTTC 
TTCCCGAGGG GGCAAAACTC CGTTACTAGT CTGGTGAAAT TGGCCCTTTC GAGCAACTTC 
CACAGTGGAT TGTTGAGTAC CGCACAAAGT TATCCTAGTC TCTCGAGTTC GAGTTCGAAT 
AATGCAAATT CGGTCGCGGT AAGTGGAGGA ACCAACAACA ATTCAGCGAG CGGAGTCGGT 
TCCAACAATG GATCCGTTCA AAGCGGAAAT GTCCAAGCCG TGTTGAATCC AGCACTGACG 
ATGAGTCTCA CCTCAACCAG TAGCGATAGT GAGCAGGTGT CTTTGGAAGA TTTCCTTGAA 
CAGTGCCGAG CTCCAACATT GCTGGGAGAT TTAGACGACG ACGAAGACAT GGAGGACGAG 
AATGATGACG ATGAAAATGA GGATGAATAC GAAGAAGTCG GAAATACTTT GCTTCAAGTG 
ATGGTTTCTC GTAATTTGTT ATCGTTTATG GATGAGGAGA CTTTGGAGAA TCGTCTGGCT 
GCCGCTGGCA AACGCAAATC GTGGGACGAC GAATTCGTGC TTAAACGCCA ATTCTCGGCA 
CTCATTCCTG CATTCGATCC GCGTCCTGGC AGGACCAACG TCAATCAAAC AAGTGATCTG 
GAAATACCTG CTCCCGGCAG TTCTACAGAC AGTAGTCATC CGAGTAGCAG CCACTCTGAG 
CATGCTCCTT TGCCTCAACC GTCCCTTGCA CTTCTTCTGC GTGGTCCCAA CATAAACGGA 
GTAAATGATG TGGAAATTCC ACTGTCGCAG CCCGATTGGA CAATATTCCG AGCGGTCCAA 
GAACTGATCC TTCAAACCAA CATGACCAAG CAGGATAAGT TCCGCAAAAT TTGGCAACCA 
ACGTACACCA TCGTCTACAG GGAAGCCTCT TCTTCGGCCG GACTCGGTTC CTGCCGCGGT 
GAGGACTTCA GCAGCGGAGA AGAAGGACGG GCCACCCCCG TCGTTTCCAT GTTTTCCCAA 
CGCAGTGGAG GATCTACCCT TTCGCCTAGC TCTCCGATCC CCGGAACACC ACTCAATCCA 
ACCGCCACCG CTCACTGCAC TGTGGACGAC GTCTTGCAGC TTCTCGGCCA ATTGAATGCC 
ATCAATCGAA CGCTTACATC TTCTCCATCC AACAACGACA AGAACCTCAT CCCGGACATG 
GAATCCAACA ATCTCAACGC GGATATATTC TTGAGCAAGA AAATTACCAA CAAACTGCAG 
CAGCAAATCC AAGACCCACT AGTCCTGGCG AGTGGAAGCC TGCCGAAATG GTGCGAAGAC 
TTCAACCAAA GCTGTCCGTT CCTGTTCCCG TTCGAGACGC GGCAGCTGTA CTTCAACTGT 
ACGGCTTTCG GCGCCTCGAG GAGCATCGTG TGGCTGCAGT CGCAGCGGGA TGTGAACCTG 
GAACGGCAAC GGGCTCCCGG GTTGAGCCCA CGGCATGCCG ATCAGCACGA GTTCCGCGTT 
GGGCGGTTGA AGCACGAGCG CGTCAAGGTT CCACGCGGTG AGAATCTACT CGAATGGGCA 
CAACAGGTTA TGAAAGTTCA CTGTAATCGC AAGTCGGTGT TGGAGGTGGA ATTCGTTGGA 
GAGGAAGGCA CTGGATTGGG TCCGACTTTG GAATTCTACG CCCTGGTTGC AGCTGAACTG 
CAGCGAAGTG ATCTGGGAAT GTGGCTGTGC GACGATGAAC CTAAGCTAAT CGAAGACGAA 
ATCGATCTAG GAGAGGGAAG CAAACCCATC GGTTATTACG TTCGTCGTTC AACCGGATTG 
TTCCCGGCGC CGTTGCCACA GGAATCTGAA ATTTGTGACT TTGTGTCAAA CTACTTCTGG 
TTCTTGGGAG TGTTTCTGGC GAAGGTACTG CAGGACGGTC GTCTAGTCGA TCTGCCACTG 
TCGAACAGCT TCTTACAACT GTTATGTAAT AACAAATCTC TCGCTAGAGG TTCACTAAGC 
GAACTTTCTT CCAAGTCTGC CTTGCACGAT GATGTGATGA TTTCTTCTTT AATGTCCGAG 
GAAAGCGATC GTGATCTGGT GGATTCATAT CAGTCAAAAT TGGCTAACGA TGGCTGTTGG 
TATGATGGAA TCCTGTCCCA AGAAAATCTC CAAGAAATCG ATCCGATTCG GTACGAGTTC 
TTGAAAGAAC TGCAAGAGTT GGTGCAGCAG AAACAAAATA TCGAACAAAA TGATGATCTC 
ACCTCCGAAG AGAAGCTACT GCAAATTAGC GAGCTTAAAT TCAACACCAA AACTGGCAGT 
GTAGCTTTGG AGGACCTCGC TCTAACGTTC ACCTATCTAC CAAGTTCGAA GAACTACGGC 
TACCAATCGG CAGATCTGAT TCCAAACGGT TCCAACATTG ATGTAACGAT CAACAACGTC 
GAAGAGTACT GCAATTTGAC GATCAACTTC TGCCTGCAAG AAGGCATTGC TAAGCAACTT 
ACTGCGTTCC ATCGAGGTTT CTGTGAAGTG TTCCCGCTGA ACAAATTGGC CGCTTTCACA 
TCGGAAGAAA TCCGCAAGAT GCTTTGTGGC GAGCAGAACC CAGAGTGGAC ACGGGAGGAC 
ATTATGACCT ACACGGAACC GAAACTCGGA TACTCCAAGG AAAGCCCTGG ATTCCTGCGT 
TTCGTGAACG TGTTGATGGG GATGAATGCC AGCGAAAGGA AAGCATTTTT GCAGTTCACC 
ACCGGCTGCA GCAGTTTACC ACCCGGTGGG CTGGCCAATT TGCACCCGCG GCTGACAGTG 
GTGCGGAAGG TAGATGCCGG CGAAGGTTCC TACCCTTCGG TCAACACCTG TGTCCACTAC 
CTCAAATTGC CGGACTATCC GAATGAGGAA ATCTTGCGGG AACGATTGCT GACGGCCACA 
AAAGAAAAGG GATTCCATCT TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021704017.1
CDS	934..9420
Translation	MGDVDPETLLEWLSMGQGDERDMQLIALEQLCMLLLMSDNVDRCFESCPPRTFLPALCKIFLDELAPENVLEVTARAITYYLDVSSECTRRIVAIDGAIKAICNRLVVADLESRTSRDLAEQCIKVLELICTREAGAVFEGGGLNCVLSFIRDNGSQIHKDTLHSAMAVVSRLCTKVEPQGANVQTCVESLSTLLQHEDPLVADGALKCFASVADRFTRKGVDPAPLAEYGLVTELLNRLSNAAGPQTSALTAGASSNATPAAQVNSQETTGTVQLSSSAPKTQGAAEAGRSSQSIATTISLLSTLCRGSPSITHDLLRSNLPDAMERAFKGDERCVLDCMRLADLILLLLFEGRQALGRVGGSSGQLAPRVRRADSSTERTHRQLIDCIRSKDTEALIEAIESGGIDVNCMDDVGQTLLNWASAFGTLEMVEFLCDKGADVNKGQRSSSLHYAACFGRPGIAKVLLKHGANPDLRDEDGKTPLDKARERPDEGHREVASILQSPGEWMTAATRSDLKTDPEDGESEPRGDPEMAPVYLKFFLPIFCKTFQSTMLASVRRSSLGLIKKMIQYVQPEMLSRLCSSEGLQSHEHSLGTLLVEVVASVLDNEDDEDGHLVVLTIIEELMSKTQDDFLDHFARLGVFSKVQALMGENGFSGGEGDNNDVIKSQDELKPSATGAQDASVVSTTGSSSSGTATTNTVEDAKEILPGKAYHWRDWSICRGRDCLYVWSDSAALELSNGSNGWFRFILDGKLATMYSSGSPENGSDSSGKGRTADSLAGEENRGEFLEKLQRARGAVRQGTPSQPILSSPSLSRIVVGNWVLQSQKEHQLHINNSEGHQVTILQDELPGFIFESNRGTKHTFTAETTLGPDFAAGWINTKKKKMRSKAEAQKYQVKNIARDLYNRYFKAAQAVPRGAVAKLCTIVRQIEAALEEQCAPKASSLLQVRIQPDASKATNSTWQEKLYNALHDLVQLLNDDGVISAYEMHSSGLVQSLVAVLSRNYWELEMNRSKANKYQKQRISIFKKCMYGDTKNGKNTASILVQKLVSVLESIEKLPVHMYDSPGGSYGLQILTKRLSFRLERAACEQTLFDRTGRNLKMEPLATVGQLNKYLLKMVAKQWYDMDRSSFLYLKKLKEAKPGSVHFKHQHDFDENGLVYYIGTNGKTTEWVNPAQYGLVTVTSSEGKQLPYGKLEDILSRDSVSVNCHTKDNKKSWFAIDLGMFIIPTAYTLRHARGYGRSALRNWMFQMSKDGINWATMLTHSDDKSLAEPGSTCTWPLECSAEEQQGWRHVRIHQNGRNASGQTHYLSLSGFEIYGKVVSVCDDMGKAAAKENEARLRKERRQIRAQLKYITQGARVVRGVDWHWDDQDGSPPGEGTVTGEIHNGWIDVKWDHGLRNSYRMGAEGKYDLKLSNSENLTAPYDMNNSGAGLVPINAGSVSSAKKVYDKSLNVLTSRKSSSTPSLPEATENKSSVASTEQATSVDNLAWKQAVEVIAENVLSCARSDLANTSGGSSSNDLSAPGSNNNNLNNQEVSVIVHSLGERGNIPDLSQINTSTSTLVSDLATITENLTLSDNIKNNISIGSSTQFVSNFGTQLAACSSSSSSEDNNKTNNINETNNKINLNNSSSSSSGKTAYLPTKLDVLDKMREGVDMLRNNTNNLLSSELLTQSNLLSSVKIALPTPQQPQQTGATAPSGPFFVASTSTSSTSTLPSGDKIVGDVKFNNTINNNTASGGVTKKVLNEVKQQEPDDRDIANNLKNNTIVVDSVEVGAAGSSKESTAESPSAAVTVNPMSVSVPNLTSNDNNASQEVQTPPGLLETFAAIARRRTSGSSVSHHVNPSSNASTASSGTSGTNNAQPNNQLSSLGSNIQAANSSFFPRGQNSVTSLVKLALSSNFHSGLLSTAQSYPSLSSSSSNNANSVAVSGGTNNNSASGVGSNNGSVQSGNVQAVLNPALTMSLTSTSSDSEQVSLEDFLEQCRAPTLLGDLDDDEDMEDENDDDENEDEYEEVGNTLLQVMVSRNLLSFMDEETLENRLAAAGKRKSWDDEFVLKRQFSALIPAFDPRPGRTNVNQTSDLEIPAPGSSTDSSHPSSSHSEHAPLPQPSLALLLRGPNINGVNDVEIPLSQPDWTIFRAVQELILQTNMTKQDKFRKIWQPTYTIVYREASSSAGLGSCRGEDFSSGEEGRATPVVSMFSQRSGGSTLSPSSPIPGTPLNPTATAHCTVDDVLQLLGQLNAINRTLTSSPSNNDKNLIPDMESNNLNADIFLSKKITNKLQQQIQDPLVLASGSLPKWCEDFNQSCPFLFPFETRQLYFNCTAFGASRSIVWLQSQRDVNLERQRAPGLSPRHADQHEFRVGRLKHERVKVPRGENLLEWAQQVMKVHCNRKSVLEVEFVGEEGTGLGPTLEFYALVAAELQRSDLGMWLCDDEPKLIEDEIDLGEGSKPIGYYVRRSTGLFPAPLPQESEICDFVSNYFWFLGVFLAKVLQDGRLVDLPLSNSFLQLLCNNKSLARGSLSELSSKSALHDDVMISSLMSEESDRDLVDSYQSKLANDGCWYDGILSQENLQEIDPIRYEFLKELQELVQQKQNIEQNDDLTSEEKLLQISELKFNTKTGSVALEDLALTFTYLPSSKNYGYQSADLIPNGSNIDVTINNVEEYCNLTINFCLQEGIAKQLTAFHRGFCEVFPLNKLAAFTSEEIRKMLCGEQNPEWTREDIMTYTEPKLGYSKESPGFLRFVNVLMGMNASERKAFLQFTTGCSSLPPGGLANLHPRLTVVRKVDAGEGSYPSVNTCVHYLKLPDYPNEEILRERLLTATKEKGFHLN

Target ORF information:

RefSeq Version	XM_021848325.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848325.1

ORF Insert Sequence:

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGGAC GACGAAGATG GCCATCTGGT GGTGCTTACC 
ATTATCGAAG AGCTCATGTC CAAGACGCAA GACGATTTCC TCGATCACTT TGCCCGGCTC 
GGAGTGTTCT CCAAAGTTCA GGCCCTGATG GGAGAAAACG GCTTCAGCGG GGGCGAAGGT 
GATAACAACG ATGTGATCAA ATCCCAAGAC GAGCTCAAAC CGTCTGCGAC CGGAGCACAA 
GATGCTTCGG TTGTCTCCAC TACTGGAAGC TCCTCCTCTG GAACTGCCAC TACTAATACG 
GTGGAAGATG CCAAAGAAAT CCTTCCCGGG AAGGCGTACC ACTGGCGAGA CTGGAGTATT 
TGTCGAGGTC GCGACTGCTT GTACGTATGG TCGGATTCAG CCGCCTTGGA GCTTTCGAAC 
GGATCCAATG GGTGGTTCCG GTTCATCCTA GACGGGAAAT TGGCCACTAT GTACTCCAGT 
GGTAGTCCAG AGAATGGCAG TGATAGCTCA GGCAAGGGAA GGACAGCGGA TTCCTTAGCC 
GGCGAAGAAA ACCGTGGTGA ATTCCTAGAA AAATTACAAC GAGCTCGCGG AGCGGTACGG 
CAGGGAACTC CCTCCCAGCC GATTCTTTCG TCGCCGAGCC TTTCGCGAAT CGTGGTCGGC 
AATTGGGTGC TGCAGAGCCA AAAAGAGCAT CAGCTACATA TCAACAACTC CGAAGGTCAC 
CAGGTGACGA TCCTGCAGGA CGAACTGCCC GGATTCATCT TCGAGAGCAA TCGCGGCACG 
AAGCATACTT TCACGGCGGA AACCACTCTA GGACCGGACT TCGCCGCCGG ATGGATCAAC 
ACCAAGAAGA AGAAGATGCG TTCGAAAGCG GAGGCTCAGA AATATCAGGT TAAAAACATC 
GCCCGAGATC TGTACAACCG GTACTTCAAA GCAGCTCAAG CCGTCCCGCG GGGCGCCGTG 
GCAAAACTGT GCACCATCGT GCGTCAAATC GAGGCAGCCC TGGAGGAACA ATGTGCCCCT 
AAGGCGTCCA GTTTGCTGCA GGTTCGGATT CAACCTGACG CTTCTAAAGC AACGAACAGC 
ACATGGCAGG AGAAGCTGTA CAACGCTCTG CACGATCTTG TGCAACTGTT GAACGACGAT 
GGAGTCATCA GTGCTTACGA GATGCACAGT TCCGGACTTG TACAATCCTT GGTGGCCGTG 
CTGTCCCGGA ACTACTGGGA ACTGGAGATG AACCGGAGCA AGGCCAACAA GTACCAGAAG 
CAACGGATAT CCATTTTCAA GAAGTGCATG TATGGCGATA CCAAAAATGG CAAAAACACG 
GCCAGCATTC TGGTGCAGAA GTTGGTATCT GTATTGGAGA GCATTGAGAA GCTTCCGGTG 
CACATGTATG ACTCCCCAGG GGGTAGCTAT GGGTTGCAGA TTCTCACAAA GCGACTGAGC 
TTCCGACTTG AGCGGGCAGC TTGTGAGCAA ACTCTCTTCG ATAGGACCGG TAGAAACCTG 
AAAATGGAAC CATTGGCAAC CGTTGGCCAG CTGAACAAGT ATCTTCTGAA AATGGTAGCA 
AAGCAGTGGT ACGATATGGA TCGATCGTCC TTCTTGTACT TGAAAAAGCT GAAAGAGGCA 
AAACCAGGAA GTGTACACTT CAAGCACCAG CACGATTTCG ACGAGAATGG TCTCGTGTAC 
TATATCGGGA CGAACGGAAA AACCACGGAA TGGGTCAATC CTGCTCAGTA CGGATTGGTT 
ACTGTCACAA GTAGCGAAGG CAAACAGCTC CCGTACGGTA AGCTGGAAGA TATCCTGTCG 
AGAGATAGCG TTAGCGTGAA TTGTCACACC AAGGATAACA AGAAATCGTG GTTCGCCATC 
GATTTGGGAA TGTTTATCAT TCCAACTGCT TATACTTTGC GACACGCCCG TGGCTATGGC 
CGCTCTGCTC TGAGAAATTG GATGTTCCAG ATGTCAAAGG ATGGAATAAA TTGGGCGACA 
ATGTTGACCC ATTCGGATGA TAAAAGTTTG GCAGAACCCG GAAGCACTTG CACTTGGCCA 
CTCGAATGCT CTGCCGAAGA ACAGCAGGGA TGGCGTCACG TGAGAATCCA TCAAAATGGA 
CGGAATGCTT CAGGGCAGAC CCATTACCTT AGCTTGAGTG GATTTGAAAT CTACGGAAAG 
GTTGTCTCGG TTTGTGATGA TATGGGAAAA GCTGCGGCCA AGGAGAATGA AGCTCGGCTT 
AGGAAAGAGC GACGACAGAT TCGAGCTCAG TTGAAGTATA TCACTCAAGG TGCACGCGTT 
GTTCGAGGAG TTGATTGGCA TTGGGATGAT CAAGATGGAA GTCCTCCTGG AGAAGGAACC 
GTAACTGGTG AGATTCATAA CGGTTGGATC GATGTAAAGT GGGACCATGG TTTAAGAAAC 
TCCTATCGCA TGGGAGCCGA AGGCAAATAT GATCTGAAGC TGTCAAACAG TGAAAACCTG 
ACTGCACCTT ACGATATGAA CAACTCCGGT GCTGGACTTG TTCCAATTAA CGCAGGAAGC 
GTGTCTTCGG CGAAGAAAGT ATATGATAAG TCGTTGAACG TTCTGACCAG TCGCAAATCG 
AGCTCTACTC CGAGCCTCCC GGAAGCAACG GAGAACAAGT CATCAGTAGC TTCGACTGAA 
CAAGCCACTT CCGTGGATAA CCTTGCATGG AAACAAGCCG TAGAAGTGAT TGCCGAAAAT 
GTGCTATCCT GTGCTCGATC TGATTTGGCC AATACCAGTG GTGGAAGTAG CAGCAATGAC 
TTATCCGCTC CCGGATCCAA CAATAATAAT CTGAACAACC AGGAAGTGTC TGTGATTGTC 
CACTCGTTGG GAGAGCGGGG AAACATCCCA GATCTGTCGC AAATCAATAC AAGTACTTCG 
ACTCTAGTCT CGGATTTGGC CACCATCACG GAGAATCTAA CACTCTCGGA TAACATCAAG 
AACAACATCA GCATCGGAAG TAGCACCCAG TTTGTGAGCA ACTTTGGAAC CCAACTTGCA 
GCTTGCTCAT CCTCGTCTTC GTCCGAAGAC AACAACAAAA CCAACAACAT CAATGAAACC 
AATAACAAAA TCAATCTCAA CAACAGCAGC AGCAGTAGTT CCGGTAAAAC GGCATACCTG 
CCAACCAAGC TCGACGTGTT GGACAAAATG AGGGAAGGCG TCGACATGCT TCGGAACAAT 
ACCAACAATT TGCTGTCCTC GGAATTGCTC ACCCAGTCAA ATCTACTCTC GTCCGTAAAG 
ATTGCACTTC CGACGCCACA ACAACCGCAG CAAACTGGAG CGACAGCTCC CTCAGGACCT 
TTCTTCGTGG CCAGCACAAG CACTAGCAGC ACGAGCACTC TCCCTTCGGG TGATAAAATC 
GTCGGAGATG TCAAGTTTAA CAATACCATC AACAATAATA CCGCTTCCGG TGGCGTAACG 
AAGAAAGTCC TGAACGAAGT CAAACAGCAG GAACCGGACG ATCGCGACAT TGCCAACAAT 
TTGAAGAACA ACACCATTGT CGTCGATTCG GTGGAAGTTG GTGCAGCAGG AAGTTCGAAG 
GAATCAACTG CTGAGTCACC TTCGGCGGCG GTCACGGTCA ATCCGATGAG TGTGAGTGTC 
CCAAATCTGA CGAGCAATGA TAACAATGCT TCACAGGAGG TACAAACTCC ACCAGGGTTG 
TTGGAAACGT TTGCGGCCAT AGCTCGAAGG CGTACTTCTG GAAGCTCTGT ATCCCATCAT 
GTGAACCCTA GCTCAAACGC TTCAACCGCT TCTTCCGGAA CCAGTGGCAC AAACAATGCT 
CAACCCAACA ATCAGCTGAG TAGCCTTGGG TCCAATATTC AAGCAGCGAA CTCTAGCTTC 
TTCCCGAGGG GGCAAAACTC CGTTACTAGT CTGGTGAAAT TGGCCCTTTC GAGCAACTTC 
CACAGTGGAT TGTTGAGTAC CGCACAAAGT TATCCTAGTC TCTCGAGTTC GAGTTCGAAT 
AATGCAAATT CGGTCGCGGT AAGTGGAGGA ACCAACAACA ATTCAGCGAG CGGAGTCGGT 
TCCAACAATG GATCCGTTCA AAGCGGAAAT GTCCAAGCCG TGTTGAATCC AGCACTGACG 
ATGAGTCTCA CCTCAACCAG TAGCGATAGT GAGCAGGTGT CTTTGGAAGA TTTCCTTGAA 
CAGTGCCGAG CTCCAACATT GCTGGGAGAT TTAGACGACG ACGAAGACAT GGAGGACGAG 
AATGATGACG ATGAAAATGA GGATGAATAC GAAGAAGTCG GAAATACTTT GCTTCAAGTG 
ATGGTTTCTC GTAATTTGTT ATCGTTTATG GATGAGGAGA CTTTGGAGAA TCGTCTGGCT 
GCCGCTGGCA AACGCAAATC GTGGGACGAC GAATTCGTGC TTAAACGCCA ATTCTCGGCA 
CTCATTCCTG CATTCGATCC GCGTCCTGGC AGGACCAACG TCAATCAAAC AAGTGATCTG 
GAAATACCTG CTCCCGGCAG TTCTACAGAC AGTAGTCATC CGAGTAGCAG CCACTCTGAG 
CATGCTCCTT TGCCTCAACC GTCCCTTGCA CTTCTTCTGC GTGGTCCCAA CATAAACGGA 
GTAAATGATG TGGAAATTCC ACTGTCGCAG CCCGATTGGA CAATATTCCG AGCGGTCCAA 
GAACTGATCC TTCAAACCAA CATGACCAAG CAGGATAAGT TCCGCAAAAT TTGGCAACCA 
ACGTACACCA TCGTCTACAG GGAAGCCTCT TCTTCGGCCG GACTCGGTTC CTGCCGCGGT 
GAGGACTTCA GCAGCGGAGA AGAAGGACGG GCCACCCCCG TCGTTTCCAT GTTTTCCCAA 
CGCAGTGGAG GATCTACCCT TTCGCCTAGC TCTCCGATCC CCGGAACACC ACTCAATCCA 
ACCGCCACCG CTCACTGCAC TGTGGACGAC GTCTTGCAGC TTCTCGGCCA ATTGAATGCC 
ATCAATCGAA CGCTTACATC TTCTCCATCC AACAACGACA AGAACCTCAT CCCGGACATG 
GAATCCAACA ATCTCAACGC GGATATATTC TTGAGCAAGA AAATTACCAA CAAACTGCAG 
CAGCAAATCC AAGACCCACT AGTCCTGGCG AGTGGAAGCC TGCCGAAATG GTGCGAAGAC 
TTCAACCAAA GCTGTCCGTT CCTGTTCCCG TTCGAGACGC GGCAGCTGTA CTTCAACTGT 
ACGGCTTTCG GCGCCTCGAG GAGCATCGTG TGGCTGCAGT CGCAGCGGGA TGTGAACCTG 
GAACGGCAAC GGGCTCCCGG GTTGAGCCCA CGGCATGCCG ATCAGCACGA GTTCCGCGTT 
GGGCGGTTGA AGCACGAGCG CGTCAAGGTT CCACGCGGTG AGAATCTACT CGAATGGGCA 
CAACAGGTTA TGAAAGTTCA CTGTAATCGC AAGTCGGTGT TGGAGGTGGA ATTCGTTGGA 
GAGGAAGGCA CTGGATTGGG TCCGACTTTG GAATTCTACG CCCTGGTTGC AGCTGAACTG 
CAGCGAAGTG ATCTGGGAAT GTGGCTGTGC GACGATGAAC CTAAGCTAAT CGAAGACGAA 
ATCGATCTAG GAGAGGGAAG CAAACCCATC GGTTATTACG TTCGTCGTTC AACCGGATTG 
TTCCCGGCGC CGTTGCCACA GGAATCTGAA ATTTGTGACT TTGTGTCAAA CTACTTCTGG 
TTCTTGGGAG TGTTTCTGGC GAAGGTACTG CAGGACGGTC GTCTAGTCGA TCTGCCACTG 
TCGAACAGCT TCTTACAACT GTTATGTAAT AACAAATCTC TCGCTAGAGG TTCACTAAGC 
GAACTTTCTT CCAAGTCTGC CTTGCACGAT GATGTGATGA TTTCTTCTTT AATGTCCGAG 
GAAAGCGATC GTGATCTGGT GGATTCATAT CAGTCAAAAT TGGCTAACGA TGGCTGTTGG 
TATGATGGAA TCCTGTCCCA AGAAAATCTC CAAGAAATCG ATCCGATTCG GTACGAGTTC 
TTGAAAGAAC TGCAAGAGTT GGTGCAGCAG AAACAAAATA TCGAACAAAA TGATGATCTC 
ACCTCCGAAG AGAAGCTACT GCAAATTAGC GAGCTTAAAT TCAACACCAA AACTGGCAGT 
GTAGCTTTGG AGGACCTCGC TCTAACGTTC ACCTATCTAC CAAGTTCGAA GAACTACGGC 
TACCAATCGG CAGATCTGAT TCCAAACGGT TCCAACATTG ATGTAACGAT CAACAACGTC 
GAAGAGTACT GCAATTTGAC GATCAACTTC TGCCTGCAAG AAGGCATTGC TAAGCAACTT 
ACTGCGTTCC ATCGAGGTTT CTGTGAAGTG TTCCCGCTGA ACAAATTGGC CGCTTTCACA 
TCGGAAGAAA TCCGCAAGAT GCTTTGTGGC GAGCAGAACC CAGAGTGGAC ACGGGAGGAC 
ATTATGACCT ACACGGAACC GAAACTCGGA TACTCCAAGG AAAGCCCTGG ATTCCTGCGT 
TTCGTGAACG TGTTGATGGG GATGAATGCC AGCGAAAGGA AAGCATTTTT GCAGTTCACC 
ACCGGCTGCA GCAGTTTACC ACCCGGTGGG CTGGCCAATT TGCACCCGCG GCTGACAGTG 
GTGCGGAAGG TAGATGCCGG CGAAGGTTCC TACCCTTCGG TCAACACCTG TGTCCACTAC 
CTCAAATTGC CGGACTATCC GAATGAGGAA ATCTTGCGGG AACGATTGCT GACGGCCACA 
AAAGAAAAGG GATTCCATCT TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL007705 ( XM_021848326.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021704018.1 Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X5, mRNA.

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CloneID	OAe124648
Clone ID Related Accession (Same CDS sequence)	XM_021848326.1
Accession Version	XM_021848326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGGAC GACGAAGATG GCCATCTGGT GGTGCTTACC 
ATTATCGAAG AGCTCATGTC CAAGACGCAA GACGATTTCC TCGATCACTT TGCCCGGCTC 
GGAGTGTTCT CCAAAGTTCA GGCCCTGATG GGAGAAAACG GCTTCAGCGG GGGCGAAGGT 
GATAACAACG ATGTGATCAA ATCCCAAGAC GAGCTCAAAC CGTCTGCGAC CGGAGCACAA 
GATGCTTCGG TTGTCTCCAC TACTGGAAGC TCCTCCTCTG GAACTGCCAC TACTAATACG 
GTGGAAGATG CCAAAGAAAT CCTTCCCGGG AAGGCGTACC ACTGGCGAGA CTGGAGTATT 
TGTCGAGGTC GCGACTGCTT GTACGTATGG TCGGATTCAG CCGCCTTGGA GCTTTCGAAC 
GGATCCAATG GGTGGTTCCG GTTCATCCTA GACGGGAAAT TGGCCACTAT GTACTCCAGT 
GGTAGTCCAG AGAATGGCAG TGATAGCTCA GAAAACCGTG GTGAATTCCT AGAAAAATTA 
CAACGAGCTC GCGGAGCGGT ACGGCAGGGA ACTCCCTCCC AGCCGATTCT TTCGTCGCCG 
AGCCTTTCGC GAATCGTGGT CGGCAATTGG GTGCTGCAGA GCCAAAAAGA GCATCAGCTA 
CATATCAACA ACTCCGAAGG TCACCAGGTG ACGATCCTGC AGGACGAACT GCCCGGATTC 
ATCTTCGAGA GCAATCGCGG CACGAAGCAT ACTTTCACGG CGGAAACCAC TCTAGGACCG 
GACTTCGCCG CCGGATGGAT CAACACCAAG AAGAAGAAGA TGCGTTCGAA AGCGGAGGCT 
CAGAAATATC AGGTTAAAAA CATCGCCCGA GATCTGTACA ACCGGTACTT CAAAGCAGCT 
CAAGCCGTCC CGCGGGGCGC CGTGGCAAAA CTGTGCACCA TCGTGCGTCA AATCGAGGCA 
GCCCTGGAGG AACAATGTGC CCCTAAGGCG TCCAGTTTGC TGCAGGTTCG GATTCAACCT 
GACGCTTCTA AAGCAACGAA CAGCACATGG CAGGAGAAGC TGTACAACGC TCTGCACGAT 
CTTGTGCAAC TGTTGAACGA CGATGGAGTC ATCAGTGCTT ACGAGATGCA CAGTTCCGGA 
CTTGTACAAT CCTTGGTGGC CGTGCTGTCC CGGAACTACT GGGAACTGGA GATGAACCGG 
AGCAAGGCCA ACAAGTACCA GAAGCAACGG ATATCCATTT TCAAGAAGTG CATGTATGGC 
GATACCAAAA ATGGCAAAAA CACGGCCAGC ATTCTGGTGC AGAAGTTGGT ATCTGTATTG 
GAGAGCATTG AGAAGCTTCC GGTGCACATG TATGACTCCC CAGGGGGTAG CTATGGGTTG 
CAGATTCTCA CAAAGCGACT GAGCTTCCGA CTTGAGCGGG CAGCTTGTGA GCAAACTCTC 
TTCGATAGGA CCGGTAGAAA CCTGAAAATG GAACCATTGG CAACCGTTGG CCAGCTGAAC 
AAGTATCTTC TGAAAATGGT AGCAAAGCAG TGGTACGATA TGGATCGATC GTCCTTCTTG 
TACTTGAAAA AGCTGAAAGA GGCAAAACCA GGAAGTGTAC ACTTCAAGCA CCAGCACGAT 
TTCGACGAGA ATGGTCTCGT GTACTATATC GGGACGAACG GAAAAACCAC GGAATGGGTC 
AATCCTGCTC AGTACGGATT GGTTACTGTC ACAAGTAGCG AAGGCAAACA GCTCCCGTAC 
GGTAAGCTGG AAGATATCCT GTCGAGAGAT AGCGTTAGCG TGAATTGTCA CACCAAGGAT 
AACAAGAAAT CGTGGTTCGC CATCGATTTG GGAATGTTTA TCATTCCAAC TGCTTATACT 
TTGCGACACG CCCGTGGCTA TGGCCGCTCT GCTCTGAGAA ATTGGATGTT CCAGATGTCA 
AAGGATGGAA TAAATTGGGC GACAATGTTG ACCCATTCGG ATGATAAAAG TTTGGCAGAA 
CCCGGAAGCA CTTGCACTTG GCCACTCGAA TGCTCTGCCG AAGAACAGCA GGGATGGCGT 
CACGTGAGAA TCCATCAAAA TGGACGGAAT GCTTCAGGGC AGACCCATTA CCTTAGCTTG 
AGTGGATTTG AAATCTACGG AAAGGTTGTC TCGGTTTGTG ATGATATGGG AAAAGCTGCG 
GCCAAGGAGA ATGAAGCTCG GCTTAGGAAA GAGCGACGAC AGATTCGAGC TCAGTTGAAG 
TATATCACTC AAGGTGCACG CGTTGTTCGA GGAGTTGATT GGCATTGGGA TGATCAAGAT 
GGAAGTCCTC CTGGAGAAGG AACCGTAACT GGTGAGATTC ATAACGGTTG GATCGATGTA 
AAGTGGGACC ATGGTTTAAG AAACTCCTAT CGCATGGGAG CCGAAGGCAA ATATGATCTG 
AAGCTGTCAA ACAGTGAAAA CCTGACTGCA CCTTACGATA TGAACAACTC CGGTGCTGGA 
CTTGTTCCAA TTAACGCAGG AAGCGTGTCT TCGGCGAAGA AAGTATATGA TAAGTCGTTG 
AACGTTCTGA CCAGTCGCAA ATCGAGCTCT ACTCCGAGCC TCCCGGAAGC AACGGAGAAC 
AAGTCATCAG TAGCTTCGAC TGAACAAGCC ACTTCCGTGG ATAACCTTGC ATGGAAACAA 
GCCGTAGAAG TGATTGCCGA AAATGTGCTA TCCTGTGCTC GATCTGATTT GGCCAATACC 
AGTGGTGGAA GTAGCAGCAA TGACTTATCC GCTCCCGGAT CCAACAATAA TAATCTGAAC 
AACCAGGAAG TGTCTGTGAT TGTCCACTCG TTGGGAGAGC GGGGAAACAT CCCAGATCTG 
TCGCAAATCA ATACAAGTAC TTCGACTCTA GTCTCGGATT TGGCCACCAT CACGGAGAAT 
CTAACACTCT CGGATAACAT CAAGAACAAC ATCAGCATCG GAAGTAGCAC CCAGTTTGTG 
AGCAACTTTG GAACCCAACT TGCAGCTTGC TCATCCTCGT CTTCGTCCGA AGACAACAAC 
AAAACCAACA ACATCAATGA AACCAATAAC AAAATCAATC TCAACAACAG CAGCAGCAGT 
AGTTCCGGTA AAACGGCATA CCTGCCAACC AAGCTCGACG TGTTGGACAA AATGAGGGAA 
GGCGTCGACA TGCTTCGGAA CAATACCAAC AATTTGCTGT CCTCGGAATT GCTCACCCAG 
TCAAATCTAC TCTCGTCCGT AAAGATTGCA CTTCCGACGC CACAACAACC GCAGCAAACT 
GGAGCGACAG CTCCCTCAGG ACCTTTCTTC GTGGCCAGCA CAAGCACTAG CAGCACGAGC 
ACTCTCCCTT CGGGTGATAA AATCGTCGGA GATGTCAAGT TTAACAATAC CATCAACAAT 
AATACCGCTT CCGGTGGCGT AACGAAGAAA GTCCTGAACG AAGTCAAACA GCAGGAACCG 
GACGATCGCG ACATTGCCAA CAATTTGAAG AACAACACCA TTGTCGTCGA TTCGGTGGAA 
GTTGGTGCAG CAGGAAGTTC GAAGGAATCA ACTGCTGAGT CACCTTCGGC GGCGGTCACG 
GTCAATCCGA TGAGTGTGAG TGTCCCAAAT CTGACGAGCA ATGATAACAA TGCTTCACAG 
GAGGTACAAA CTCCACCAGG GTTGTTGGAA ACGTTTGCGG CCATAGCTCG AAGGCGTACT 
TCTGGAAGCT CTGTATCCCA TCATGTGAAC CCTAGCTCAA ACGCTTCAAC CGCTTCTTCC 
GGAACCAGTG GCACAAACAA TGCTCAACCC AACAATCAGC TGAGTAGCCT TGGGTCCAAT 
ATTCAAGCAG CGAACTCTAG CTTCTTCCCG AGGGGGCAAA ACTCCGTTAC TAGTCTGGTG 
AAATTGGCCC TTTCGAGCAA CTTCCACAGT GGATTGTTGA GTACCGCACA AAGTTATCCT 
AGTCTCTCGA GTTCGAGTTC GAATAATGCA AATTCGGTCG CGGTAAGTGG AGGAACCAAC 
AACAATTCAG CGAGCGGAGT CGGTTCCAAC AATGGATCCG TTCAAAGCGG AAATGTCCAA 
GCCGTGTTGA ATCCAGCACT GACGATGAGT CTCACCTCAA CCAGTAGCGA TAGTGAGCAG 
GTGTCTTTGG AAGATTTCCT TGAACAGTGC CGAGCTCCAA CATTGCTGGG AGATTTAGAC 
GACGACGAAG ACATGGAGGA CGAGAATGAT GACGATGAAA ATGAGGATGA ATACGAAGAA 
GTCGGAAATA CTTTGCTTCA AGTGATGGTT TCTCGTAATT TGTTATCGTT TATGGATGAG 
GAGACTTTGG AGAATCGTCT GGCTGCCGCT GGCAAACGCA AATCGTGGGA CGACGAATTC 
GTGCTTAAAC GCCAATTCTC GGCACTCATT CCTGCATTCG ATCCGCGTCC TGGCAGGACC 
AACGTCAATC AAACAAGTGA TCTGGAAATA CCTGCTCCCG GCAGTTCTAC AGACAGTAGT 
CATCCGAGTA GCAGCCACTC TGAGCATGCT CCTTTGCCTC AACCGTCCCT TGCACTTCTT 
CTGCGTGGTC CCAACATAAA CGGAGTAAAT GATGTGGAAA TTCCACTGTC GCAGCCCGAT 
TGGACAATAT TCCGAGCGGT CCAAGAACTG ATCCTTCAAA CCAACATGAC CAAGCAGGAT 
AAGTTCCGCA AAATTTGGCA ACCAACGTAC ACCATCGTCT ACAGGGAAGC CTCTTCTTCG 
GCCGGACTCG GTTCCTGCCG CGGTGAGGAC TTCAGCAGCG GAGAAGAAGG ACGGGCCACC 
CCCGTCGTTT CCATGTTTTC CCAACGCAGT GGAGGATCTA CCCTTTCGCC TAGCTCTCCG 
ATCCCCGGAA CACCACTCAA TCCAACCGCC ACCGCTCACT GCACTGTGGA CGACGTCTTG 
CAGCTTCTCG GCCAATTGAA TGCCATCAAT CGAACGCTTA CATCTTCTCC ATCCAACAAC 
GACAAGAACC TCATCCCGGA CATGGAATCC AACAATCTCA ACGCGGATAT ATTCTTGAGC 
AAGAAAATTA CCAACAAACT GCAGCAGCAA ATCCAAGACC CACTAGTCCT GGCGAGTGGA 
AGCCTGCCGA AATGGTGCGA AGACTTCAAC CAAAGCTGTC CGTTCCTGTT CCCGTTCGAG 
ACGCGGCAGC TGTACTTCAA CTGTACGGCT TTCGGCGCCT CGAGGAGCAT CGTGTGGCTG 
CAGTCGCAGC GGGATGTGAA CCTGGAACGG CAACGGGCTC CCGGGTTGAG CCCACGGCAT 
GCCGATCAGC ACGAGTTCCG CGTTGGGCGG TTGAAGCACG AGCGCGTCAA GGTTCCACGC 
GGTGAGAATC TACTCGAATG GGCACAACAG GTTATGAAAG TTCACTGTAA TCGCAAGTCG 
GTGTTGGAGG TGGAATTCGT TGGAGAGGAA GGCACTGGAT TGGGTCCGAC TTTGGAATTC 
TACGCCCTGG TTGCAGCTGA ACTGCAGCGA AGTGATCTGG GAATGTGGCT GTGCGACGAT 
GAACCTAAGC TAATCGAAGA CGAAATCGAT CTAGGAGAGG GAAGCAAACC CATCGGTTAT 
TACGTTCGTC GTTCAACCGG ATTGTTCCCG GCGCCGTTGC CACAGGAATC TGAAATTTGT 
GACTTTGTGT CAAACTACTT CTGGTTCTTG GGAGTGTTTC TGGCGAAGGT ACTGCAGGAC 
GGTCGTCTAG TCGATCTGCC ACTGTCGAAC AGCTTCTTAC AACTGTTATG TAATAACAAA 
TCTCTCGCTA GAGGTTCACT AAGCGAACTT TCTTCCAAGT CTGCCTTGCA CGATGATGTG 
ATGATTTCTT CTTTAATGTC CGAGGAAAGC GATCGTGATC TGGTGGATTC ATATCAGTCA 
AAATTGGCTA ACGATGGCTG TTGGTATGAT GGAATCCTGT CCCAAGAAAA TCTCCAAGAA 
ATCGATCCGA TTCGGTACGA GTTCTTGAAA GAACTGCAAG AGTTGGTGCA GCAGAAACAA 
AATATCGAAC AAAATGATGA TCTCACCTCC GAAGAGAAGC TACTGCAAAT TAGCGAGCTT 
AAATTCAACA CCAAAACTGG CAGTGTAGCT TTGGAGGACC TCGCTCTAAC GTTCACCTAT 
CTACCAAGTT CGAAGAACTA CGGCTACCAA TCGGCAGATC TGATTCCAAA CGGTTCCAAC 
ATTGATGTAA CGATCAACAA CGTCGAAGAG TACTGCAATT TGACGATCAA CTTCTGCCTG 
CAAGAAGGCA TTGCTAAGCA ACTTACTGCG TTCCATCGAG GTTTCTGTGA AGTGTTCCCG 
CTGAACAAAT TGGCCGCTTT CACATCGGAA GAAATCCGCA AGATGCTTTG TGGCGAGCAG 
AACCCAGAGT GGACACGGGA GGACATTATG ACCTACACGG AACCGAAACT CGGATACTCC 
AAGGAAAGCC CTGGATTCCT GCGTTTCGTG AACGTGTTGA TGGGGATGAA TGCCAGCGAA 
AGGAAAGCAT TTTTGCAGTT CACCACCGGC TGCAGCAGTT TACCACCCGG TGGGCTGGCC 
AATTTGCACC CGCGGCTGAC AGTGGTGCGG AAGGTAGATG CCGGCGAAGG TTCCTACCCT 
TCGGTCAACA CCTGTGTCCA CTACCTCAAA TTGCCGGACT ATCCGAATGA GGAAATCTTG 
CGGGAACGAT TGCTGACGGC CACAAAAGAA AAGGGATTCC ATCTTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021704018.1
CDS	934..9384
Translation	MGDVDPETLLEWLSMGQGDERDMQLIALEQLCMLLLMSDNVDRCFESCPPRTFLPALCKIFLDELAPENVLEVTARAITYYLDVSSECTRRIVAIDGAIKAICNRLVVADLESRTSRDLAEQCIKVLELICTREAGAVFEGGGLNCVLSFIRDNGSQIHKDTLHSAMAVVSRLCTKVEPQGANVQTCVESLSTLLQHEDPLVADGALKCFASVADRFTRKGVDPAPLAEYGLVTELLNRLSNAAGPQTSALTAGASSNATPAAQVNSQETTGTVQLSSSAPKTQGAAEAGRSSQSIATTISLLSTLCRGSPSITHDLLRSNLPDAMERAFKGDERCVLDCMRLADLILLLLFEGRQALGRVGGSSGQLAPRVRRADSSTERTHRQLIDCIRSKDTEALIEAIESGGIDVNCMDDVGQTLLNWASAFGTLEMVEFLCDKGADVNKGQRSSSLHYAACFGRPGIAKVLLKHGANPDLRDEDGKTPLDKARERPDEGHREVASILQSPGEWMTAATRSDLKTDPEDGESEPRGDPEMAPVYLKFFLPIFCKTFQSTMLASVRRSSLGLIKKMIQYVQPEMLSRLCSSEGLQSHEHSLGTLLVEVVASVLDNEDDEDGHLVVLTIIEELMSKTQDDFLDHFARLGVFSKVQALMGENGFSGGEGDNNDVIKSQDELKPSATGAQDASVVSTTGSSSSGTATTNTVEDAKEILPGKAYHWRDWSICRGRDCLYVWSDSAALELSNGSNGWFRFILDGKLATMYSSGSPENGSDSSENRGEFLEKLQRARGAVRQGTPSQPILSSPSLSRIVVGNWVLQSQKEHQLHINNSEGHQVTILQDELPGFIFESNRGTKHTFTAETTLGPDFAAGWINTKKKKMRSKAEAQKYQVKNIARDLYNRYFKAAQAVPRGAVAKLCTIVRQIEAALEEQCAPKASSLLQVRIQPDASKATNSTWQEKLYNALHDLVQLLNDDGVISAYEMHSSGLVQSLVAVLSRNYWELEMNRSKANKYQKQRISIFKKCMYGDTKNGKNTASILVQKLVSVLESIEKLPVHMYDSPGGSYGLQILTKRLSFRLERAACEQTLFDRTGRNLKMEPLATVGQLNKYLLKMVAKQWYDMDRSSFLYLKKLKEAKPGSVHFKHQHDFDENGLVYYIGTNGKTTEWVNPAQYGLVTVTSSEGKQLPYGKLEDILSRDSVSVNCHTKDNKKSWFAIDLGMFIIPTAYTLRHARGYGRSALRNWMFQMSKDGINWATMLTHSDDKSLAEPGSTCTWPLECSAEEQQGWRHVRIHQNGRNASGQTHYLSLSGFEIYGKVVSVCDDMGKAAAKENEARLRKERRQIRAQLKYITQGARVVRGVDWHWDDQDGSPPGEGTVTGEIHNGWIDVKWDHGLRNSYRMGAEGKYDLKLSNSENLTAPYDMNNSGAGLVPINAGSVSSAKKVYDKSLNVLTSRKSSSTPSLPEATENKSSVASTEQATSVDNLAWKQAVEVIAENVLSCARSDLANTSGGSSSNDLSAPGSNNNNLNNQEVSVIVHSLGERGNIPDLSQINTSTSTLVSDLATITENLTLSDNIKNNISIGSSTQFVSNFGTQLAACSSSSSSEDNNKTNNINETNNKINLNNSSSSSSGKTAYLPTKLDVLDKMREGVDMLRNNTNNLLSSELLTQSNLLSSVKIALPTPQQPQQTGATAPSGPFFVASTSTSSTSTLPSGDKIVGDVKFNNTINNNTASGGVTKKVLNEVKQQEPDDRDIANNLKNNTIVVDSVEVGAAGSSKESTAESPSAAVTVNPMSVSVPNLTSNDNNASQEVQTPPGLLETFAAIARRRTSGSSVSHHVNPSSNASTASSGTSGTNNAQPNNQLSSLGSNIQAANSSFFPRGQNSVTSLVKLALSSNFHSGLLSTAQSYPSLSSSSSNNANSVAVSGGTNNNSASGVGSNNGSVQSGNVQAVLNPALTMSLTSTSSDSEQVSLEDFLEQCRAPTLLGDLDDDEDMEDENDDDENEDEYEEVGNTLLQVMVSRNLLSFMDEETLENRLAAAGKRKSWDDEFVLKRQFSALIPAFDPRPGRTNVNQTSDLEIPAPGSSTDSSHPSSSHSEHAPLPQPSLALLLRGPNINGVNDVEIPLSQPDWTIFRAVQELILQTNMTKQDKFRKIWQPTYTIVYREASSSAGLGSCRGEDFSSGEEGRATPVVSMFSQRSGGSTLSPSSPIPGTPLNPTATAHCTVDDVLQLLGQLNAINRTLTSSPSNNDKNLIPDMESNNLNADIFLSKKITNKLQQQIQDPLVLASGSLPKWCEDFNQSCPFLFPFETRQLYFNCTAFGASRSIVWLQSQRDVNLERQRAPGLSPRHADQHEFRVGRLKHERVKVPRGENLLEWAQQVMKVHCNRKSVLEVEFVGEEGTGLGPTLEFYALVAAELQRSDLGMWLCDDEPKLIEDEIDLGEGSKPIGYYVRRSTGLFPAPLPQESEICDFVSNYFWFLGVFLAKVLQDGRLVDLPLSNSFLQLLCNNKSLARGSLSELSSKSALHDDVMISSLMSEESDRDLVDSYQSKLANDGCWYDGILSQENLQEIDPIRYEFLKELQELVQQKQNIEQNDDLTSEEKLLQISELKFNTKTGSVALEDLALTFTYLPSSKNYGYQSADLIPNGSNIDVTINNVEEYCNLTINFCLQEGIAKQLTAFHRGFCEVFPLNKLAAFTSEEIRKMLCGEQNPEWTREDIMTYTEPKLGYSKESPGFLRFVNVLMGMNASERKAFLQFTTGCSSLPPGGLANLHPRLTVVRKVDAGEGSYPSVNTCVHYLKLPDYPNEEILRERLLTATKEKGFHLN

Target ORF information:

RefSeq Version	XM_021848326.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti E3 ubiquitin-protein ligase HECTD1 (LOC5569522), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021848326.1

ORF Insert Sequence:

ATGGGTGACG TTGATCCAGA AACGCTGCTA GAATGGCTGT CGATGGGGCA GGGAGACGAA 
CGGGATATGC AGTTGATTGC CCTGGAGCAG CTATGTATGC TTCTGCTGAT GTCCGACAAC 
GTTGATCGTT GTTTCGAAAG TTGTCCCCCG CGAACGTTCC TCCCAGCGCT GTGTAAAATA 
TTTTTAGATG AACTAGCGCC AGAGAATGTA CTGGAAGTGA CAGCACGTGC CATCACCTAC 
TATCTAGACG TATCATCCGA GTGCACACGT CGCATTGTAG CGATCGATGG TGCGATCAAA 
GCTATCTGCA ACCGGTTGGT GGTGGCCGAT CTCGAAAGCA GAACTAGTCG TGATCTTGCG 
GAGCAGTGCA TCAAAGTACT TGAGCTCATA TGCACTCGAG AAGCAGGGGC CGTGTTTGAA 
GGAGGTGGAC TTAATTGTGT GCTTTCATTC ATTCGGGACA ATGGGTCTCA GATCCACAAA 
GACACACTCC ACTCGGCAAT GGCCGTGGTG TCCAGGCTAT GTACCAAGGT GGAGCCACAA 
GGTGCTAATG TACAAACTTG TGTAGAAAGT CTAAGTACTC TGCTACAGCA CGAAGATCCG 
CTCGTAGCAG ACGGAGCATT GAAATGCTTT GCTTCGGTGG CAGATCGATT TACTAGAAAA 
GGAGTTGATC CTGCCCCACT TGCAGAGTAT GGACTAGTAA CTGAGTTGTT AAATCGACTA 
AGCAACGCTG CAGGACCACA AACATCAGCC TTGACAGCTG GAGCTAGTTC AAATGCGACG 
CCCGCTGCAC AGGTCAACAG TCAAGAGACT ACAGGAACAG TGCAGCTTTC ATCGTCAGCA 
CCCAAAACAC AAGGAGCAGC CGAAGCAGGG CGATCGAGTC AATCGATCGC CACAACTATA 
TCACTGTTAT CTACCTTGTG TCGAGGGTCA CCATCGATCA CGCATGATCT CCTCCGATCC 
AATCTACCAG ATGCTATGGA ACGAGCCTTC AAAGGTGATG AACGATGTGT TCTCGACTGT 
ATGAGGCTTG CCGATCTCAT CCTGCTGCTG TTGTTCGAAG GTCGACAAGC TCTTGGCAGG 
GTGGGTGGAT CTAGCGGTCA GTTAGCTCCT CGTGTGCGAA GAGCAGACTC TAGTACTGAG 
CGAACACATC GACAGTTGAT CGATTGCATA CGTAGTAAGG ACACCGAAGC CCTAATCGAA 
GCTATCGAAT CGGGTGGAAT CGATGTCAAC TGCATGGACG ATGTCGGTCA AACATTACTT 
AACTGGGCTT CGGCCTTTGG TACATTAGAA ATGGTAGAGT TTTTATGTGA TAAAGGTGCT 
GACGTCAACA AAGGCCAGCG AAGTTCCTCT CTGCATTATG CGGCCTGTTT CGGTCGACCC 
GGCATAGCAA AAGTACTGCT CAAACACGGT GCCAATCCTG ACCTACGGGA CGAAGACGGC 
AAAACACCAC TGGACAAGGC TCGCGAACGG CCGGATGAAG GCCACCGTGA AGTTGCTTCC 
ATTCTTCAGT CGCCTGGAGA ATGGATGACC GCGGCAACAC GATCGGACTT GAAAACCGAC 
CCCGAAGACG GGGAAAGCGA GCCCCGCGGA GATCCGGAGA TGGCACCCGT CTATCTCAAG 
TTCTTTCTAC CAATATTCTG CAAGACGTTC CAAAGCACAA TGCTAGCCAG CGTTCGACGG 
TCTAGTTTAG GTCTGATCAA GAAGATGATT CAATATGTCC AACCAGAAAT GCTGTCGAGG 
CTTTGTTCCT CGGAGGGCCT CCAAAGTCAT GAGCACAGCC TAGGAACTTT ACTAGTCGAA 
GTCGTCGCCA GTGTTTTGGA CAACGAGGAC GACGAAGATG GCCATCTGGT GGTGCTTACC 
ATTATCGAAG AGCTCATGTC CAAGACGCAA GACGATTTCC TCGATCACTT TGCCCGGCTC 
GGAGTGTTCT CCAAAGTTCA GGCCCTGATG GGAGAAAACG GCTTCAGCGG GGGCGAAGGT 
GATAACAACG ATGTGATCAA ATCCCAAGAC GAGCTCAAAC CGTCTGCGAC CGGAGCACAA 
GATGCTTCGG TTGTCTCCAC TACTGGAAGC TCCTCCTCTG GAACTGCCAC TACTAATACG 
GTGGAAGATG CCAAAGAAAT CCTTCCCGGG AAGGCGTACC ACTGGCGAGA CTGGAGTATT 
TGTCGAGGTC GCGACTGCTT GTACGTATGG TCGGATTCAG CCGCCTTGGA GCTTTCGAAC 
GGATCCAATG GGTGGTTCCG GTTCATCCTA GACGGGAAAT TGGCCACTAT GTACTCCAGT 
GGTAGTCCAG AGAATGGCAG TGATAGCTCA GAAAACCGTG GTGAATTCCT AGAAAAATTA 
CAACGAGCTC GCGGAGCGGT ACGGCAGGGA ACTCCCTCCC AGCCGATTCT TTCGTCGCCG 
AGCCTTTCGC GAATCGTGGT CGGCAATTGG GTGCTGCAGA GCCAAAAAGA GCATCAGCTA 
CATATCAACA ACTCCGAAGG TCACCAGGTG ACGATCCTGC AGGACGAACT GCCCGGATTC 
ATCTTCGAGA GCAATCGCGG CACGAAGCAT ACTTTCACGG CGGAAACCAC TCTAGGACCG 
GACTTCGCCG CCGGATGGAT CAACACCAAG AAGAAGAAGA TGCGTTCGAA AGCGGAGGCT 
CAGAAATATC AGGTTAAAAA CATCGCCCGA GATCTGTACA ACCGGTACTT CAAAGCAGCT 
CAAGCCGTCC CGCGGGGCGC CGTGGCAAAA CTGTGCACCA TCGTGCGTCA AATCGAGGCA 
GCCCTGGAGG AACAATGTGC CCCTAAGGCG TCCAGTTTGC TGCAGGTTCG GATTCAACCT 
GACGCTTCTA AAGCAACGAA CAGCACATGG CAGGAGAAGC TGTACAACGC TCTGCACGAT 
CTTGTGCAAC TGTTGAACGA CGATGGAGTC ATCAGTGCTT ACGAGATGCA CAGTTCCGGA 
CTTGTACAAT CCTTGGTGGC CGTGCTGTCC CGGAACTACT GGGAACTGGA GATGAACCGG 
AGCAAGGCCA ACAAGTACCA GAAGCAACGG ATATCCATTT TCAAGAAGTG CATGTATGGC 
GATACCAAAA ATGGCAAAAA CACGGCCAGC ATTCTGGTGC AGAAGTTGGT ATCTGTATTG 
GAGAGCATTG AGAAGCTTCC GGTGCACATG TATGACTCCC CAGGGGGTAG CTATGGGTTG 
CAGATTCTCA CAAAGCGACT GAGCTTCCGA CTTGAGCGGG CAGCTTGTGA GCAAACTCTC 
TTCGATAGGA CCGGTAGAAA CCTGAAAATG GAACCATTGG CAACCGTTGG CCAGCTGAAC 
AAGTATCTTC TGAAAATGGT AGCAAAGCAG TGGTACGATA TGGATCGATC GTCCTTCTTG 
TACTTGAAAA AGCTGAAAGA GGCAAAACCA GGAAGTGTAC ACTTCAAGCA CCAGCACGAT 
TTCGACGAGA ATGGTCTCGT GTACTATATC GGGACGAACG GAAAAACCAC GGAATGGGTC 
AATCCTGCTC AGTACGGATT GGTTACTGTC ACAAGTAGCG AAGGCAAACA GCTCCCGTAC 
GGTAAGCTGG AAGATATCCT GTCGAGAGAT AGCGTTAGCG TGAATTGTCA CACCAAGGAT 
AACAAGAAAT CGTGGTTCGC CATCGATTTG GGAATGTTTA TCATTCCAAC TGCTTATACT 
TTGCGACACG CCCGTGGCTA TGGCCGCTCT GCTCTGAGAA ATTGGATGTT CCAGATGTCA 
AAGGATGGAA TAAATTGGGC GACAATGTTG ACCCATTCGG ATGATAAAAG TTTGGCAGAA 
CCCGGAAGCA CTTGCACTTG GCCACTCGAA TGCTCTGCCG AAGAACAGCA GGGATGGCGT 
CACGTGAGAA TCCATCAAAA TGGACGGAAT GCTTCAGGGC AGACCCATTA CCTTAGCTTG 
AGTGGATTTG AAATCTACGG AAAGGTTGTC TCGGTTTGTG ATGATATGGG AAAAGCTGCG 
GCCAAGGAGA ATGAAGCTCG GCTTAGGAAA GAGCGACGAC AGATTCGAGC TCAGTTGAAG 
TATATCACTC AAGGTGCACG CGTTGTTCGA GGAGTTGATT GGCATTGGGA TGATCAAGAT 
GGAAGTCCTC CTGGAGAAGG AACCGTAACT GGTGAGATTC ATAACGGTTG GATCGATGTA 
AAGTGGGACC ATGGTTTAAG AAACTCCTAT CGCATGGGAG CCGAAGGCAA ATATGATCTG 
AAGCTGTCAA ACAGTGAAAA CCTGACTGCA CCTTACGATA TGAACAACTC CGGTGCTGGA 
CTTGTTCCAA TTAACGCAGG AAGCGTGTCT TCGGCGAAGA AAGTATATGA TAAGTCGTTG 
AACGTTCTGA CCAGTCGCAA ATCGAGCTCT ACTCCGAGCC TCCCGGAAGC AACGGAGAAC 
AAGTCATCAG TAGCTTCGAC TGAACAAGCC ACTTCCGTGG ATAACCTTGC ATGGAAACAA 
GCCGTAGAAG TGATTGCCGA AAATGTGCTA TCCTGTGCTC GATCTGATTT GGCCAATACC 
AGTGGTGGAA GTAGCAGCAA TGACTTATCC GCTCCCGGAT CCAACAATAA TAATCTGAAC 
AACCAGGAAG TGTCTGTGAT TGTCCACTCG TTGGGAGAGC GGGGAAACAT CCCAGATCTG 
TCGCAAATCA ATACAAGTAC TTCGACTCTA GTCTCGGATT TGGCCACCAT CACGGAGAAT 
CTAACACTCT CGGATAACAT CAAGAACAAC ATCAGCATCG GAAGTAGCAC CCAGTTTGTG 
AGCAACTTTG GAACCCAACT TGCAGCTTGC TCATCCTCGT CTTCGTCCGA AGACAACAAC 
AAAACCAACA ACATCAATGA AACCAATAAC AAAATCAATC TCAACAACAG CAGCAGCAGT 
AGTTCCGGTA AAACGGCATA CCTGCCAACC AAGCTCGACG TGTTGGACAA AATGAGGGAA 
GGCGTCGACA TGCTTCGGAA CAATACCAAC AATTTGCTGT CCTCGGAATT GCTCACCCAG 
TCAAATCTAC TCTCGTCCGT AAAGATTGCA CTTCCGACGC CACAACAACC GCAGCAAACT 
GGAGCGACAG CTCCCTCAGG ACCTTTCTTC GTGGCCAGCA CAAGCACTAG CAGCACGAGC 
ACTCTCCCTT CGGGTGATAA AATCGTCGGA GATGTCAAGT TTAACAATAC CATCAACAAT 
AATACCGCTT CCGGTGGCGT AACGAAGAAA GTCCTGAACG AAGTCAAACA GCAGGAACCG 
GACGATCGCG ACATTGCCAA CAATTTGAAG AACAACACCA TTGTCGTCGA TTCGGTGGAA 
GTTGGTGCAG CAGGAAGTTC GAAGGAATCA ACTGCTGAGT CACCTTCGGC GGCGGTCACG 
GTCAATCCGA TGAGTGTGAG TGTCCCAAAT CTGACGAGCA ATGATAACAA TGCTTCACAG 
GAGGTACAAA CTCCACCAGG GTTGTTGGAA ACGTTTGCGG CCATAGCTCG AAGGCGTACT 
TCTGGAAGCT CTGTATCCCA TCATGTGAAC CCTAGCTCAA ACGCTTCAAC CGCTTCTTCC 
GGAACCAGTG GCACAAACAA TGCTCAACCC AACAATCAGC TGAGTAGCCT TGGGTCCAAT 
ATTCAAGCAG CGAACTCTAG CTTCTTCCCG AGGGGGCAAA ACTCCGTTAC TAGTCTGGTG 
AAATTGGCCC TTTCGAGCAA CTTCCACAGT GGATTGTTGA GTACCGCACA AAGTTATCCT 
AGTCTCTCGA GTTCGAGTTC GAATAATGCA AATTCGGTCG CGGTAAGTGG AGGAACCAAC 
AACAATTCAG CGAGCGGAGT CGGTTCCAAC AATGGATCCG TTCAAAGCGG AAATGTCCAA 
GCCGTGTTGA ATCCAGCACT GACGATGAGT CTCACCTCAA CCAGTAGCGA TAGTGAGCAG 
GTGTCTTTGG AAGATTTCCT TGAACAGTGC CGAGCTCCAA CATTGCTGGG AGATTTAGAC 
GACGACGAAG ACATGGAGGA CGAGAATGAT GACGATGAAA ATGAGGATGA ATACGAAGAA 
GTCGGAAATA CTTTGCTTCA AGTGATGGTT TCTCGTAATT TGTTATCGTT TATGGATGAG 
GAGACTTTGG AGAATCGTCT GGCTGCCGCT GGCAAACGCA AATCGTGGGA CGACGAATTC 
GTGCTTAAAC GCCAATTCTC GGCACTCATT CCTGCATTCG ATCCGCGTCC TGGCAGGACC 
AACGTCAATC AAACAAGTGA TCTGGAAATA CCTGCTCCCG GCAGTTCTAC AGACAGTAGT 
CATCCGAGTA GCAGCCACTC TGAGCATGCT CCTTTGCCTC AACCGTCCCT TGCACTTCTT 
CTGCGTGGTC CCAACATAAA CGGAGTAAAT GATGTGGAAA TTCCACTGTC GCAGCCCGAT 
TGGACAATAT TCCGAGCGGT CCAAGAACTG ATCCTTCAAA CCAACATGAC CAAGCAGGAT 
AAGTTCCGCA AAATTTGGCA ACCAACGTAC ACCATCGTCT ACAGGGAAGC CTCTTCTTCG 
GCCGGACTCG GTTCCTGCCG CGGTGAGGAC TTCAGCAGCG GAGAAGAAGG ACGGGCCACC 
CCCGTCGTTT CCATGTTTTC CCAACGCAGT GGAGGATCTA CCCTTTCGCC TAGCTCTCCG 
ATCCCCGGAA CACCACTCAA TCCAACCGCC ACCGCTCACT GCACTGTGGA CGACGTCTTG 
CAGCTTCTCG GCCAATTGAA TGCCATCAAT CGAACGCTTA CATCTTCTCC ATCCAACAAC 
GACAAGAACC TCATCCCGGA CATGGAATCC AACAATCTCA ACGCGGATAT ATTCTTGAGC 
AAGAAAATTA CCAACAAACT GCAGCAGCAA ATCCAAGACC CACTAGTCCT GGCGAGTGGA 
AGCCTGCCGA AATGGTGCGA AGACTTCAAC CAAAGCTGTC CGTTCCTGTT CCCGTTCGAG 
ACGCGGCAGC TGTACTTCAA CTGTACGGCT TTCGGCGCCT CGAGGAGCAT CGTGTGGCTG 
CAGTCGCAGC GGGATGTGAA CCTGGAACGG CAACGGGCTC CCGGGTTGAG CCCACGGCAT 
GCCGATCAGC ACGAGTTCCG CGTTGGGCGG TTGAAGCACG AGCGCGTCAA GGTTCCACGC 
GGTGAGAATC TACTCGAATG GGCACAACAG GTTATGAAAG TTCACTGTAA TCGCAAGTCG 
GTGTTGGAGG TGGAATTCGT TGGAGAGGAA GGCACTGGAT TGGGTCCGAC TTTGGAATTC 
TACGCCCTGG TTGCAGCTGA ACTGCAGCGA AGTGATCTGG GAATGTGGCT GTGCGACGAT 
GAACCTAAGC TAATCGAAGA CGAAATCGAT CTAGGAGAGG GAAGCAAACC CATCGGTTAT 
TACGTTCGTC GTTCAACCGG ATTGTTCCCG GCGCCGTTGC CACAGGAATC TGAAATTTGT 
GACTTTGTGT CAAACTACTT CTGGTTCTTG GGAGTGTTTC TGGCGAAGGT ACTGCAGGAC 
GGTCGTCTAG TCGATCTGCC ACTGTCGAAC AGCTTCTTAC AACTGTTATG TAATAACAAA 
TCTCTCGCTA GAGGTTCACT AAGCGAACTT TCTTCCAAGT CTGCCTTGCA CGATGATGTG 
ATGATTTCTT CTTTAATGTC CGAGGAAAGC GATCGTGATC TGGTGGATTC ATATCAGTCA 
AAATTGGCTA ACGATGGCTG TTGGTATGAT GGAATCCTGT CCCAAGAAAA TCTCCAAGAA 
ATCGATCCGA TTCGGTACGA GTTCTTGAAA GAACTGCAAG AGTTGGTGCA GCAGAAACAA 
AATATCGAAC AAAATGATGA TCTCACCTCC GAAGAGAAGC TACTGCAAAT TAGCGAGCTT 
AAATTCAACA CCAAAACTGG CAGTGTAGCT TTGGAGGACC TCGCTCTAAC GTTCACCTAT 
CTACCAAGTT CGAAGAACTA CGGCTACCAA TCGGCAGATC TGATTCCAAA CGGTTCCAAC 
ATTGATGTAA CGATCAACAA CGTCGAAGAG TACTGCAATT TGACGATCAA CTTCTGCCTG 
CAAGAAGGCA TTGCTAAGCA ACTTACTGCG TTCCATCGAG GTTTCTGTGA AGTGTTCCCG 
CTGAACAAAT TGGCCGCTTT CACATCGGAA GAAATCCGCA AGATGCTTTG TGGCGAGCAG 
AACCCAGAGT GGACACGGGA GGACATTATG ACCTACACGG AACCGAAACT CGGATACTCC 
AAGGAAAGCC CTGGATTCCT GCGTTTCGTG AACGTGTTGA TGGGGATGAA TGCCAGCGAA 
AGGAAAGCAT TTTTGCAGTT CACCACCGGC TGCAGCAGTT TACCACCCGG TGGGCTGGCC 
AATTTGCACC CGCGGCTGAC AGTGGTGCGG AAGGTAGATG CCGGCGAAGG TTCCTACCCT 
TCGGTCAACA CCTGTGTCCA CTACCTCAAA TTGCCGGACT ATCCGAATGA GGAAATCTTG 
CGGGAACGAT TGCTGACGGC CACAAAAGAA AAGGGATTCC ATCTTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

AAEL_AAEL007705 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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