AAEL_AAEL005334 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL005334
Entrez Gene ID	5566334
Full Name	titin
Synonyms	AAEL005333,AAEL005334
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021841052.1	XP_021696744.1	titin
XM_001650679.1	XP_001650729.1	AAEL005334-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL005334 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL005334 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe02449	XM_001650679.1 Latest version!	Aedes aegypti AAEL005334-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OAe119286	XM_021841052.1 Latest version!	Aedes aegypti titin (LOC5566334), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL005334 ( XM_001650679.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001650729.1 Aedes aegypti AAEL005334-RA partial mRNA.

Price & Availability↑

CloneID	OAe02449
Clone ID Related Accession (Same CDS sequence)	XM_001650679.1
Accession Version	XM_001650679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL005334-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810361). COMPLETENESS: incomplete on both ends.

ATGCTGGTTC CTGGAACTCC GAACAATTCG GCACCGGATG TCGTGACGCT TCGATGGAAA 
CGGCCGACCA GTGATGGCGG GTCGCCAATT CTCGGCTATA TAATTGAGCA TCGACGAACA 
GGTTCGCCGC ACTGGGTTCG TGCCTCTGCT CAGCTGATCC AGGCCACTGA GCTGTCCTTG 
AGTGGACTCG AACCAGGATG GCGCTACCAG TTCCGGGTAA CGGCACAAAA TATCGTTGGA 
CAATCGGATT GCAGTGAGCT ATCCGATCCG TTGACGGTGA CCCTACAAAG GGCGGCAATT 
GCTCCACCAC GGTTCGTTCA AGAACTGTAC GATACAACTG CCATCGAAAA TGAGAAAATT 
GAATTCAGAG TCTCGGTTGC CGGAAGCCCT GCGCCTCAGA TCGGCTGGTA TAAGGATGGG 
TTCGAGATAT TCAGTAGTCG ACGAACGAAG ATCATTACCG AAGCGGACTT AAGCATACTT 
GTGATCCATC AAGCAGCGTT GACGGATGAA GGAGAGTTCA AATGCACTGC AACGAATCGC 
GCAGGTCACG CTGTAACCAT ATGTAGTCTA TCTATCGACG CGTATCCTAA GATACGTCTT 
CCTAGACAGT ACGAAGAAGG CTTGATTGTT GAAGCCGACG AAGTAATACG TCTTAAAGTA 
GGTGTAGCCG GTCAACCTAC TCCAATAGTC GAGTGGAGCC ACAACGGTGA ACTCATAGAA 
AACGGTGGTC GCTACGAGAT CGAAACCAAC GACAAGAACT CGTCCCTTCG AATCGCCAAC 
GCCCAAAGAA GTGACCGTGG CGAATATAAC CTGAGGGCGT TCAACAAGCT AGGTGAAGAC 
TATGCATATT TCTTGGTAAC TGTCACAGCT AAGCCAGAAC CTCCGAGCAA AGTTCAGATC 
AGCATCGGTC TTAGCAAAGC CATCACCCTC AGTTGGTCTG CCCCTGAAGA TGACGGTGGC 
TGCAAGATCG GTAACTACAT AGTAGAATAC TATCGAGTCG GATGGGATGT CTGGCTCAAG 
GCAACCACCT GTCGACAACT CACCACTACC CTCAGTGGAC TCTTCGAAGG TTCCCAATAC 
CGATTCCGAG TGAAGGCAGA AAATCCGTAC GGCCTGAGTG AACCTTCGGA AGAATCCGAC 
ACGCTCTTCA TCCCGGATCC GAAACGCGGA ATCAACAGTG CCACGCAAAT TCCGGAACCC 
GCACCCCCAG AAGACGAACC CACCCCGCAA GCCATCCCTC GTAAACGCCG AGACGTTTCT 
CACTCACCAA CCAGGGAAGC CGAACATATC AAGAAGGTGA AAGCCTTCAC CCCAGAAACC 
TACAGCCGGG ATGAAATCGT CCGCGAAATG TCCTACGGAA CTCGGGACGA AGACTTCTTC 
CAACCGAAGC AATCCACCGA AGCACCACCC AAACCACCAG CCGTCGTTAT CACCGAACCA 
GCACCAACCC CCAAGCAAGA ACCCACCCCG CCTCAATCGC CGAAACCCCA ACGACCCCAA 
TCGTTCCCAT CCCCACTGAA AGCGATGGAG AAGTTCTCCA AGGAGGCCAG TCCACTCTTC 
AGTCGGCCGT CCCCATCCCC ATCGGACAAT CAGCTGTCCC CGCAGCCGAT CTCGCAGCCG 
ACGTCCCCAT CCGACGGTGC CAAGTCGCCG ACCATGCACA ACAGCAGCGA GTTCATGCTG 
GTCCTGTACA ACGAACCGGA AGCGAAAAAT AATACACGCA AAATCCATTT CATCAAGTTC 
ATCAGTTCGT CGAAACAGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001650729.1
CDS	1..1764
Translation	MLVPGTPNNSAPDVVTLRWKRPTSDGGSPILGYIIEHRRTGSPHWVRASAQLIQATELSLSGLEPGWRYQFRVTAQNIVGQSDCSELSDPLTVTLQRAAIAPPRFVQELYDTTAIENEKIEFRVSVAGSPAPQIGWYKDGFEIFSSRRTKIITEADLSILVIHQAALTDEGEFKCTATNRAGHAVTICSLSIDAYPKIRLPRQYEEGLIVEADEVIRLKVGVAGQPTPIVEWSHNGELIENGGRYEIETNDKNSSLRIANAQRSDRGEYNLRAFNKLGEDYAYFLVTVTAKPEPPSKVQISIGLSKAITLSWSAPEDDGGCKIGNYIVEYYRVGWDVWLKATTCRQLTTTLSGLFEGSQYRFRVKAENPYGLSEPSEESDTLFIPDPKRGINSATQIPEPAPPEDEPTPQAIPRKRRDVSHSPTREAEHIKKVKAFTPETYSRDEIVREMSYGTRDEDFFQPKQSTEAPPKPPAVVITEPAPTPKQEPTPPQSPKPQRPQSFPSPLKAMEKFSKEASPLFSRPSPSPSDNQLSPQPISQPTSPSDGAKSPTMHNSSEFMLVLYNEPEAKNNTRKIHFIKFISSSKQM

Target ORF information:

RefSeq Version	XM_001650679.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL005334-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001650679.1

ORF Insert Sequence:

ATGCTGGTTC CTGGAACTCC GAACAATTCG GCACCGGATG TCGTGACGCT TCGATGGAAA 
CGGCCGACCA GTGATGGCGG GTCGCCAATT CTCGGCTATA TAATTGAGCA TCGACGAACA 
GGTTCGCCGC ACTGGGTTCG TGCCTCTGCT CAGCTGATCC AGGCCACTGA GCTGTCCTTG 
AGTGGACTCG AACCAGGATG GCGCTACCAG TTCCGGGTAA CGGCACAAAA TATCGTTGGA 
CAATCGGATT GCAGTGAGCT ATCCGATCCG TTGACGGTGA CCCTACAAAG GGCGGCAATT 
GCTCCACCAC GGTTCGTTCA AGAACTGTAC GATACAACTG CCATCGAAAA TGAGAAAATT 
GAATTCAGAG TCTCGGTTGC CGGAAGCCCT GCGCCTCAGA TCGGCTGGTA TAAGGATGGG 
TTCGAGATAT TCAGTAGTCG ACGAACGAAG ATCATTACCG AAGCGGACTT AAGCATACTT 
GTGATCCATC AAGCAGCGTT GACGGATGAA GGAGAGTTCA AATGCACTGC AACGAATCGC 
GCAGGTCACG CTGTAACCAT ATGTAGTCTA TCTATCGACG CGTATCCTAA GATACGTCTT 
CCTAGACAGT ACGAAGAAGG CTTGATTGTT GAAGCCGACG AAGTAATACG TCTTAAAGTA 
GGTGTAGCCG GTCAACCTAC TCCAATAGTC GAGTGGAGCC ACAACGGTGA ACTCATAGAA 
AACGGTGGTC GCTACGAGAT CGAAACCAAC GACAAGAACT CGTCCCTTCG AATCGCCAAC 
GCCCAAAGAA GTGACCGTGG CGAATATAAC CTGAGGGCGT TCAACAAGCT AGGTGAAGAC 
TATGCATATT TCTTGGTAAC TGTCACAGCT AAGCCAGAAC CTCCGAGCAA AGTTCAGATC 
AGCATCGGTC TTAGCAAAGC CATCACCCTC AGTTGGTCTG CCCCTGAAGA TGACGGTGGC 
TGCAAGATCG GTAACTACAT AGTAGAATAC TATCGAGTCG GATGGGATGT CTGGCTCAAG 
GCAACCACCT GTCGACAACT CACCACTACC CTCAGTGGAC TCTTCGAAGG TTCCCAATAC 
CGATTCCGAG TGAAGGCAGA AAATCCGTAC GGCCTGAGTG AACCTTCGGA AGAATCCGAC 
ACGCTCTTCA TCCCGGATCC GAAACGCGGA ATCAACAGTG CCACGCAAAT TCCGGAACCC 
GCACCCCCAG AAGACGAACC CACCCCGCAA GCCATCCCTC GTAAACGCCG AGACGTTTCT 
CACTCACCAA CCAGGGAAGC CGAACATATC AAGAAGGTGA AAGCCTTCAC CCCAGAAACC 
TACAGCCGGG ATGAAATCGT CCGCGAAATG TCCTACGGAA CTCGGGACGA AGACTTCTTC 
CAACCGAAGC AATCCACCGA AGCACCACCC AAACCACCAG CCGTCGTTAT CACCGAACCA 
GCACCAACCC CCAAGCAAGA ACCCACCCCG CCTCAATCGC CGAAACCCCA ACGACCCCAA 
TCGTTCCCAT CCCCACTGAA AGCGATGGAG AAGTTCTCCA AGGAGGCCAG TCCACTCTTC 
AGTCGGCCGT CCCCATCCCC ATCGGACAAT CAGCTGTCCC CGCAGCCGAT CTCGCAGCCG 
ACGTCCCCAT CCGACGGTGC CAAGTCGCCG ACCATGCACA ACAGCAGCGA GTTCATGCTG 
GTCCTGTACA ACGAACCGGA AGCGAAAAAT AATACACGCA AAATCCATTT CATCAAGTTC 
ATCAGTTCGT CGAAACAGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL005334 ( XM_021841052.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021696744.1 Aedes aegypti titin (LOC5566334), mRNA.

Price & Availability↑

CloneID	OAe119286
Clone ID Related Accession (Same CDS sequence)	XM_021841052.1
Accession Version	XM_021841052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	titin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATC AACCAACGAA ACACTCCGGA AAGCCGAAGA AGGCCGTCCA CTGGAAGTCA 
GCAGAGCCGC CTTCACCCCC GGGGAAGCCT ATGCTGGTTC CTGGAACTCC GAACAATTCG 
GCACCGGATG TCGTGACGCT TCGATGGAAA CGGCCCACCA GTGATGGCGG GTCGCCAATT 
CTCGGCTATA TAATTGAACA TCGGCGAACA GGATCGCCAC ACTGGGTTCG TGCCTCTGCT 
CAGCTGATCC AGGCCACTGA GCTGTCCTTG AGTGGACTCG AACCAGGGTG GCGTTACCAG 
TTCCGGGTAA CGGCTCAAAA TATCGTTGGA CAATCGGATT GCAGTGAATT ATCCGATCCG 
TTGACGGTGA CCCTACAAAG GGCGGCCATT GCTCCACCAC GGTTCGTTCA AGAACTGTAC 
GATACAACTG CCATCGAAAA TGAGAAAATT GAATTCCGAG TCTCGGTTGC CGGAAGTCCT 
GCGCCACAGA TCGGCTGGTA TAAGGATGGG TTCGAGATAT TCAGTAGTCG GCGAACGAAG 
ATCATTACCG AAGCGGACTT GAGCATACTT GTGATCCATC AGGCAGCGTT GACGGATGAA 
GGAGAGTTCA AATGCACTGC AACGAATCGC GCAGGTCACG CTGTAACCAT ATGTAGTCTA 
TCTATCGACG CGTATCCTAA GATACGTCTT CCTAGACAGT ACGAAGAAGG CTTGATTGTT 
GAAGCCGACG AAGTAATACG TCTTAAAGTA GGTGTAGCCG GTCAACCTAC TCCAATAGTC 
GAGTGGAGCC ACAACGGTGA ACTCATAGAA AACGGTGGTC GCTACGAGAT CGAAACCAAC 
GACAAGAACT CGTCCCTTCG AATCGCCAAC GCCCAAAGAA GTGATCGTGG CGAATATAAC 
CTGCGGGCGT TCAACAAGCT AGGTGAAGAC TACGCATACT TCTTGGTAAC TGTCACAGCT 
AAGCCAGAAC CTCCGAGCAA AGTTCAGATC AGCATCGGTC TTAGCAAAGC CATCACCCTC 
AGTTGGTCTG CCCCTGAAGA CGACGGTGGC TGCAAGATCG GTAACTACAT AGTAGAATAC 
TATCGAGTCG GATGGGATGT CTGGCTCAAG GCAACCACCT GTCGACAACT CACCACTACC 
CTCAGTGGAC TCTTCGAAGG TTCCCAATAC CGATTCCGAG TGAAGGCAGA AAATCCGTAC 
GGCCTGAGTG AACCTTCCGA AGAATCCGAC ACGCTCTTCA TTCCGGATCC GAAGCGCGGA 
ATCAACAGTG CCACGCAAAT TCCGGAACCC GCACCCCCAG AAGACGAACC CACCCCGCAA 
GCCATCCCTC GTAAACGCCG AGACGTTTCT CACTCACCAA CCAGGGAAGC CGAACATATC 
AAGAAGGTGA AAGCCTTCAC CCCGGAAACT TACAGCCGGG ACGAAATCGT CCGCGAAATG 
TCCTACGGAA CTCGGGACGA AGACTTCTTC CAACCGAAGC AATCCACCGA AGCACCACCC 
AAACCACCAG CCGTCGTTAT CACCGAACCA GCACCAACAC CCAAGCAAGA ACCCACCCCG 
CCTCCATCTC CAAAACCGCA ACGACCCCAA TCGTTCCCAT CCCCACTGAA AGCGATGGAG 
AAATTCTCCA AGGAGGCCAG TCCACTCTTC AGTCGGCCGT CCCCATCCCC GTCGGACAAT 
CAGCTGTCCC CGCAGCCGAT CTCGCAGCCG ACGTCCCCAT CCGACGGTGC CAAGTCGCCG 
ACCATGCACA ACAGCAGCGA GTTCATGCTG GTCCTGTACA ACGAACCGGA AGCGAAAAAT 
AATACACGAA ACTCAACCTT CGATTTTGAG CTGGAGGACC TGGCAGCTCC GCCACCCCCG 
CTATCTCTGT CGGCTCCGGA GCTAAACGTG GAACCACCAC CAGCCCCGAG AATGCGCCCT 
GCAGTCAGTT CCACGGAGTT GCTCTACGAG CGGGCGATGG CACGATTCTA CCAAGCAGTC 
GCCATGGAGG AAACTGAAAA CCAACGCAAG GAAGCCGAAC GGAGAAGAAG CATAAGCGTT 
GTCGAGACGC AGCATCAGCA GCAGCAGCAA CAACTACAGC AGCAACCAGA AACAGCGGGA 
GTGGTGAGTC GGGTGTCGGA TCGCAGGAGC AGCCTTCGAC GGCGTCTGTC TGGGGATAAG 
GAAACGATCG TCAAGCAGTC TAGTTTCGAA TCGCAACATT CCAGTACCGT GGATGATGTG 
ATACCAGAGG AGAAGGACGA GGTAGATAAG GTAAAGGATT TGGGTGAAGA AGCGGGAGCA 
GTGGCTATGG ATAAGGTGCC GGATTTGGAC GAGGAGCTGT CAATATCTTC GTCAATGGAT 
TCAATGCTGG AGGAGTTGAA GCGGAGGGAG TCGGCACGAT CGAGTCGATC GAGGATAATG 
AGTGATGACG AAGAAATGGA GACGTATCAT CCGGGACCGA GAGTAATGTC TCCGTACAGG 
AATCCTGATC CAAGCCAGGC CGTTCAAGTG TTAACGAAGC CATTACCGAT GCCGGATCCG 
AACTTTGTTC CGAAGCCTAT TTTGAAACGA CCGTCAACAG AGATCTTGAC TAAAGACGAG 
AAAGAGAAAA AGGTTGCTCC GAGTAAACCG GAGCGCAAGA GTTTGTCCAA TATCTTTGAC 
CGCAAGTCTC CGGCGAAAGA AGAGCGGAAG CCAAGTCCAA TTCGAACTGC TCCTCAGGTG 
GAGAAGGAAA AGACACCCGA AGTAGAGGAG AAGAAACCGG AACCACCACA GTCTCCAGAG 
CCTTCAAGTG AGAAGCAAGC ACCTACGGGT CAGGAGATCA ACACAGCAAT CAAATCGGAA 
GCTGCTCGTC AGAGACGATT GGAATCTAGG CAGAGCTCTA TCGAAGAGTC TAGGGCAGTG 
GCTGATTTCT ACAGCGAAAT CGTCCAGCAA GTCGAAACTG CCAAGAAACC CCGTCAGTTG 
CCTTTGTACA TGAACCCGGA CGAGGCTCGT AAATTGAATG AACGAGGGCG TTCTCGGGAG 
ACTTCGTATG AACGGTTAGT TGGGTCTCGC TCTGGATCAA GGTCTCCCAT GCCTCCGGAA 
GAAACCCATC GGGCTCAAAA ATCTCGGTCG CCTTCGCTGA CTTCAGAGCA AACTATAACG 
AAAGGAAAAC CTGCACGGGA GAGCCGTATT CTTCGGCAGC GATCGGCCTC GATCGACAAC 
GACACCAGCC GACCATCGTC CGCGATGAAA GAGCATCCTC CAATGCCACA GACTCAACCG 
GAAACTGCAT TTAACTCTAG CGGAGCTATC GAAGAACCAA CGAGGCGCAG TCGACCATTG 
CGTGAGTCTA CAGCGATCGC CAAGAAGCGA AATGTTTCTC AGACACGCTC CCGCAGCAGT 
TCAGGAGTTC GCGATGGAGT TGCAACTCCG GAAAAGACTC CAATGGACGT AGCTAGGCGT 
GCACGACTTC GTAGTGCTTC CAAGTCCCCT GCGGCACTAC AGCGAACTCC CATAGCTGAG 
CCACTTCCAG AACCACCGAA GCCACCGCTA ACTCCTTCAG TCGCTCCAAC GCTTCCAGTC 
ACTTCAACGC CTTCAGTTCC ACCAACGCCT TCAACCCCTG TTGAACAACC GAAACCGCAA 
GAGCAACGAT CCCGCTCACA GTCCCAGGCT CCTGAATCAC CCCCTGCAGA AACCACTTAC 
CAATCCACTG TATCCTACCT AACGGACGTG GCACTGTTTG CCCTCGCCTG TTGGTTATAC 
CTCTTCAAGA ACCCCAAGCT AGCGATCCCG ATCATCGTAC TGATCATGTA CCGCCACATT 
AGGGATGCCC TCCGGCATCG GATGCCACCG TGGATGAAGC GAACCCTGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021696744.1
CDS	358..4191
Translation	MGNQPTKHSGKPKKAVHWKSAEPPSPPGKPMLVPGTPNNSAPDVVTLRWKRPTSDGGSPILGYIIEHRRTGSPHWVRASAQLIQATELSLSGLEPGWRYQFRVTAQNIVGQSDCSELSDPLTVTLQRAAIAPPRFVQELYDTTAIENEKIEFRVSVAGSPAPQIGWYKDGFEIFSSRRTKIITEADLSILVIHQAALTDEGEFKCTATNRAGHAVTICSLSIDAYPKIRLPRQYEEGLIVEADEVIRLKVGVAGQPTPIVEWSHNGELIENGGRYEIETNDKNSSLRIANAQRSDRGEYNLRAFNKLGEDYAYFLVTVTAKPEPPSKVQISIGLSKAITLSWSAPEDDGGCKIGNYIVEYYRVGWDVWLKATTCRQLTTTLSGLFEGSQYRFRVKAENPYGLSEPSEESDTLFIPDPKRGINSATQIPEPAPPEDEPTPQAIPRKRRDVSHSPTREAEHIKKVKAFTPETYSRDEIVREMSYGTRDEDFFQPKQSTEAPPKPPAVVITEPAPTPKQEPTPPPSPKPQRPQSFPSPLKAMEKFSKEASPLFSRPSPSPSDNQLSPQPISQPTSPSDGAKSPTMHNSSEFMLVLYNEPEAKNNTRNSTFDFELEDLAAPPPPLSLSAPELNVEPPPAPRMRPAVSSTELLYERAMARFYQAVAMEETENQRKEAERRRSISVVETQHQQQQQQLQQQPETAGVVSRVSDRRSSLRRRLSGDKETIVKQSSFESQHSSTVDDVIPEEKDEVDKVKDLGEEAGAVAMDKVPDLDEELSISSSMDSMLEELKRRESARSSRSRIMSDDEEMETYHPGPRVMSPYRNPDPSQAVQVLTKPLPMPDPNFVPKPILKRPSTEILTKDEKEKKVAPSKPERKSLSNIFDRKSPAKEERKPSPIRTAPQVEKEKTPEVEEKKPEPPQSPEPSSEKQAPTGQEINTAIKSEAARQRRLESRQSSIEESRAVADFYSEIVQQVETAKKPRQLPLYMNPDEARKLNERGRSRETSYERLVGSRSGSRSPMPPEETHRAQKSRSPSLTSEQTITKGKPARESRILRQRSASIDNDTSRPSSAMKEHPPMPQTQPETAFNSSGAIEEPTRRSRPLRESTAIAKKRNVSQTRSRSSSGVRDGVATPEKTPMDVARRARLRSASKSPAALQRTPIAEPLPEPPKPPLTPSVAPTLPVTSTPSVPPTPSTPVEQPKPQEQRSRSQSQAPESPPAETTYQSTVSYLTDVALFALACWLYLFKNPKLAIPIIVLIMYRHIRDALRHRMPPWMKRTLS

Target ORF information:

RefSeq Version	XM_021841052.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti titin (LOC5566334), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021841052.1

ORF Insert Sequence:

ATGGGAAATC AACCAACGAA ACACTCCGGA AAGCCGAAGA AGGCCGTCCA CTGGAAGTCA 
GCAGAGCCGC CTTCACCCCC GGGGAAGCCT ATGCTGGTTC CTGGAACTCC GAACAATTCG 
GCACCGGATG TCGTGACGCT TCGATGGAAA CGGCCCACCA GTGATGGCGG GTCGCCAATT 
CTCGGCTATA TAATTGAACA TCGGCGAACA GGATCGCCAC ACTGGGTTCG TGCCTCTGCT 
CAGCTGATCC AGGCCACTGA GCTGTCCTTG AGTGGACTCG AACCAGGGTG GCGTTACCAG 
TTCCGGGTAA CGGCTCAAAA TATCGTTGGA CAATCGGATT GCAGTGAATT ATCCGATCCG 
TTGACGGTGA CCCTACAAAG GGCGGCCATT GCTCCACCAC GGTTCGTTCA AGAACTGTAC 
GATACAACTG CCATCGAAAA TGAGAAAATT GAATTCCGAG TCTCGGTTGC CGGAAGTCCT 
GCGCCACAGA TCGGCTGGTA TAAGGATGGG TTCGAGATAT TCAGTAGTCG GCGAACGAAG 
ATCATTACCG AAGCGGACTT GAGCATACTT GTGATCCATC AGGCAGCGTT GACGGATGAA 
GGAGAGTTCA AATGCACTGC AACGAATCGC GCAGGTCACG CTGTAACCAT ATGTAGTCTA 
TCTATCGACG CGTATCCTAA GATACGTCTT CCTAGACAGT ACGAAGAAGG CTTGATTGTT 
GAAGCCGACG AAGTAATACG TCTTAAAGTA GGTGTAGCCG GTCAACCTAC TCCAATAGTC 
GAGTGGAGCC ACAACGGTGA ACTCATAGAA AACGGTGGTC GCTACGAGAT CGAAACCAAC 
GACAAGAACT CGTCCCTTCG AATCGCCAAC GCCCAAAGAA GTGATCGTGG CGAATATAAC 
CTGCGGGCGT TCAACAAGCT AGGTGAAGAC TACGCATACT TCTTGGTAAC TGTCACAGCT 
AAGCCAGAAC CTCCGAGCAA AGTTCAGATC AGCATCGGTC TTAGCAAAGC CATCACCCTC 
AGTTGGTCTG CCCCTGAAGA CGACGGTGGC TGCAAGATCG GTAACTACAT AGTAGAATAC 
TATCGAGTCG GATGGGATGT CTGGCTCAAG GCAACCACCT GTCGACAACT CACCACTACC 
CTCAGTGGAC TCTTCGAAGG TTCCCAATAC CGATTCCGAG TGAAGGCAGA AAATCCGTAC 
GGCCTGAGTG AACCTTCCGA AGAATCCGAC ACGCTCTTCA TTCCGGATCC GAAGCGCGGA 
ATCAACAGTG CCACGCAAAT TCCGGAACCC GCACCCCCAG AAGACGAACC CACCCCGCAA 
GCCATCCCTC GTAAACGCCG AGACGTTTCT CACTCACCAA CCAGGGAAGC CGAACATATC 
AAGAAGGTGA AAGCCTTCAC CCCGGAAACT TACAGCCGGG ACGAAATCGT CCGCGAAATG 
TCCTACGGAA CTCGGGACGA AGACTTCTTC CAACCGAAGC AATCCACCGA AGCACCACCC 
AAACCACCAG CCGTCGTTAT CACCGAACCA GCACCAACAC CCAAGCAAGA ACCCACCCCG 
CCTCCATCTC CAAAACCGCA ACGACCCCAA TCGTTCCCAT CCCCACTGAA AGCGATGGAG 
AAATTCTCCA AGGAGGCCAG TCCACTCTTC AGTCGGCCGT CCCCATCCCC GTCGGACAAT 
CAGCTGTCCC CGCAGCCGAT CTCGCAGCCG ACGTCCCCAT CCGACGGTGC CAAGTCGCCG 
ACCATGCACA ACAGCAGCGA GTTCATGCTG GTCCTGTACA ACGAACCGGA AGCGAAAAAT 
AATACACGAA ACTCAACCTT CGATTTTGAG CTGGAGGACC TGGCAGCTCC GCCACCCCCG 
CTATCTCTGT CGGCTCCGGA GCTAAACGTG GAACCACCAC CAGCCCCGAG AATGCGCCCT 
GCAGTCAGTT CCACGGAGTT GCTCTACGAG CGGGCGATGG CACGATTCTA CCAAGCAGTC 
GCCATGGAGG AAACTGAAAA CCAACGCAAG GAAGCCGAAC GGAGAAGAAG CATAAGCGTT 
GTCGAGACGC AGCATCAGCA GCAGCAGCAA CAACTACAGC AGCAACCAGA AACAGCGGGA 
GTGGTGAGTC GGGTGTCGGA TCGCAGGAGC AGCCTTCGAC GGCGTCTGTC TGGGGATAAG 
GAAACGATCG TCAAGCAGTC TAGTTTCGAA TCGCAACATT CCAGTACCGT GGATGATGTG 
ATACCAGAGG AGAAGGACGA GGTAGATAAG GTAAAGGATT TGGGTGAAGA AGCGGGAGCA 
GTGGCTATGG ATAAGGTGCC GGATTTGGAC GAGGAGCTGT CAATATCTTC GTCAATGGAT 
TCAATGCTGG AGGAGTTGAA GCGGAGGGAG TCGGCACGAT CGAGTCGATC GAGGATAATG 
AGTGATGACG AAGAAATGGA GACGTATCAT CCGGGACCGA GAGTAATGTC TCCGTACAGG 
AATCCTGATC CAAGCCAGGC CGTTCAAGTG TTAACGAAGC CATTACCGAT GCCGGATCCG 
AACTTTGTTC CGAAGCCTAT TTTGAAACGA CCGTCAACAG AGATCTTGAC TAAAGACGAG 
AAAGAGAAAA AGGTTGCTCC GAGTAAACCG GAGCGCAAGA GTTTGTCCAA TATCTTTGAC 
CGCAAGTCTC CGGCGAAAGA AGAGCGGAAG CCAAGTCCAA TTCGAACTGC TCCTCAGGTG 
GAGAAGGAAA AGACACCCGA AGTAGAGGAG AAGAAACCGG AACCACCACA GTCTCCAGAG 
CCTTCAAGTG AGAAGCAAGC ACCTACGGGT CAGGAGATCA ACACAGCAAT CAAATCGGAA 
GCTGCTCGTC AGAGACGATT GGAATCTAGG CAGAGCTCTA TCGAAGAGTC TAGGGCAGTG 
GCTGATTTCT ACAGCGAAAT CGTCCAGCAA GTCGAAACTG CCAAGAAACC CCGTCAGTTG 
CCTTTGTACA TGAACCCGGA CGAGGCTCGT AAATTGAATG AACGAGGGCG TTCTCGGGAG 
ACTTCGTATG AACGGTTAGT TGGGTCTCGC TCTGGATCAA GGTCTCCCAT GCCTCCGGAA 
GAAACCCATC GGGCTCAAAA ATCTCGGTCG CCTTCGCTGA CTTCAGAGCA AACTATAACG 
AAAGGAAAAC CTGCACGGGA GAGCCGTATT CTTCGGCAGC GATCGGCCTC GATCGACAAC 
GACACCAGCC GACCATCGTC CGCGATGAAA GAGCATCCTC CAATGCCACA GACTCAACCG 
GAAACTGCAT TTAACTCTAG CGGAGCTATC GAAGAACCAA CGAGGCGCAG TCGACCATTG 
CGTGAGTCTA CAGCGATCGC CAAGAAGCGA AATGTTTCTC AGACACGCTC CCGCAGCAGT 
TCAGGAGTTC GCGATGGAGT TGCAACTCCG GAAAAGACTC CAATGGACGT AGCTAGGCGT 
GCACGACTTC GTAGTGCTTC CAAGTCCCCT GCGGCACTAC AGCGAACTCC CATAGCTGAG 
CCACTTCCAG AACCACCGAA GCCACCGCTA ACTCCTTCAG TCGCTCCAAC GCTTCCAGTC 
ACTTCAACGC CTTCAGTTCC ACCAACGCCT TCAACCCCTG TTGAACAACC GAAACCGCAA 
GAGCAACGAT CCCGCTCACA GTCCCAGGCT CCTGAATCAC CCCCTGCAGA AACCACTTAC 
CAATCCACTG TATCCTACCT AACGGACGTG GCACTGTTTG CCCTCGCCTG TTGGTTATAC 
CTCTTCAAGA ACCCCAAGCT AGCGATCCCG ATCATCGTAC TGATCATGTA CCGCCACATT 
AGGGATGCCC TCCGGCATCG GATGCCACCG TGGATGAAGC GAACCCTGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL005334 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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