LOC5564186 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5564186
Entrez Gene ID	5564186
Full Name	protein toll
Synonyms	AAEL007613,AAEL014337,AeToll1,TOLL1A,Toll1
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001648474.1	XP_001648524.1	AAEL014337-PA
XM_021853596.1	XP_021709288.1	protein toll
XM_021853026.1	XP_021708718.1	protein toll

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5564186 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5564186 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe00556	XM_001648474.1 Latest version!	Aedes aegypti AAEL014337-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OAe130896	XM_021853026.1 Latest version!	Aedes aegypti protein toll (LOC5564186), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe130896	XM_021853596.1 Latest version!	Aedes aegypti protein toll (LOC5564186), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5564186 ( XM_001648474.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001648524.1 Aedes aegypti AAEL014337-RA partial mRNA.

Price & Availability↑

CloneID	OAe00556
Clone ID Related Accession (Same CDS sequence)	XM_001648474.1
Accession Version	XM_001648474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL014337-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001809891). COMPLETENESS: incomplete on both ends.

ATGGCACGGG CGCTCCCATT ACTGGCACTG TTACTCACGC ACAATTTGGC AAGTTCATTT 
CCGCAGCACC ACTACACGGA CTACGTGCTG GGCGGAGAAC TGGTCAGTGG AGGAGGGGCT 
GGCGGAACCA CGACGGGAAT TGGATCCAGT GGCACAGGCG GGACCGGCAC CGGCGCCGGT 
GAACAACGAT TCACATGTCC CGAACCGACG ACCACCACTA GCGATTGCAC TTGCATGTAC 
GCCAGCTCCG ATTACGAAAT TCAATGTCCC GTGATTAATC CGGAAATTAC GGTGAAAATA 
AAACCTGGCA TCTACGCCCA GATACAGTGC TACGAGAAGC AGGAGTTTAG CGACCTGCCA 
ACGTTGAATG TTGGCAACAC GACCCAGGTC AAAATCATCC ATTGCCCGCT GCCACAGCGA 
CAGTCCATTA AGCAGTTCAT CAAATTCCTG GGAGTTAACC ACGTGAAGGA TTTCTGGTAT 
CAGAATTATG GGAAGGATTT CGGAGTGCGA TTGATCCGGC AGCATTTCGA GGGTATGCAT 
GATTTGGAAA AACTTTTCCT CAGCTCAGGA ATCGAAGACA TCCAACCGGA TCTGTTTGCC 
GATCTGCCGA ACCTGAAGTG GCTCATTCTA CGAAGCAATC ATGTCAAACT CCTGCACAAC 
GTTTTCGATA ACCTGACTAA CTTGATTATA CTGGAACTGG GAGCCAACCA GATCACCGAG 
CTTGAACCGG GTCTGTTCAA GAATCAACGG AAACTACGAC ATCTCAACTT GTGGCGCAAT 
CAGCTGCGTA ACATCTCCAA AGAATCTTTC CGCGGAGCGG AAACACTCCA AGAGCTAGAT 
CTCTCCGTCA ACGCGATCGA AACGCTCAAT TCCGATGTGT TCACGTTGCT TCCGGATCTC 
ACTGTCCTGA ATCTCGGGTT CAATCGATTC ACCGTTCTAC CCGATAATCT CCTTTCCGAA 
AACCGCAAGC TGCGGGAGTT CAAATTCATC AATAATCAGG CTCCGCTGCA AACCCTACCG 
GAAGCCTTTC TCGGCAACCT CCCTCAGCTG AAATCGGTCA TCTTGAATCG CTGCAGCTTC 
ACGCATCTGC CCGCATCGCT GTTCCGCGGA TCGAGCGAAA TCACCCACAT GGATCTGAGC 
TACAACCAGC TGGGATCCGT TCCGGAGTTG CTTCTCCGCG ATCAGCTGCG TCTTCAAGAT 
CTGAATTTGG CATACAACGA ACTGGAATCG CTTCCGGAAC ACTTGCTGGA GAACACCCGG 
GAGTTGCTCA CCCTGCGACT GTCGTACAAC CGGCTCTACA ATCTTTCGGC AAACATCTTC 
GCCTCGCTGG ACAAGCTCAC CGAACTGCAC CTGAACGACA ACTTCCTGCA CGCCATCGAT 
CGGCTCACCT TCAGCAACAC CAAAGCCCTC GGAAAGCTGT ACATGCAAAA CAACCACCTC 
TCCTTCCACG ATTTCAGTTT CGTCCTCCAG GAGCAGGACA TCGCCGACAC CGATGGCACA 
CCGTTCCAAT ACCTGGAAAA CCTTCAAATC CTAAACCTCC GCAACAACAG CATCACCACC 
ATCTTCCGTG ATTGGAACTA CAACAACCTG CATCTGCGCG AGCTCGATCT CAGCTACAAC 
AACATCTCCA CCCTGTCCTA TCTGAGCCTA CAGATGTCCG CGTCAATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001648524.1
CDS	1..1671
Translation	MARALPLLALLLTHNLASSFPQHHYTDYVLGGELVSGGGAGGTTTGIGSSGTGGTGTGAGEQRFTCPEPTTTTSDCTCMYASSDYEIQCPVINPEITVKIKPGIYAQIQCYEKQEFSDLPTLNVGNTTQVKIIHCPLPQRQSIKQFIKFLGVNHVKDFWYQNYGKDFGVRLIRQHFEGMHDLEKLFLSSGIEDIQPDLFADLPNLKWLILRSNHVKLLHNVFDNLTNLIILELGANQITELEPGLFKNQRKLRHLNLWRNQLRNISKESFRGAETLQELDLSVNAIETLNSDVFTLLPDLTVLNLGFNRFTVLPDNLLSENRKLREFKFINNQAPLQTLPEAFLGNLPQLKSVILNRCSFTHLPASLFRGSSEITHMDLSYNQLGSVPELLLRDQLRLQDLNLAYNELESLPEHLLENTRELLTLRLSYNRLYNLSANIFASLDKLTELHLNDNFLHAIDRLTFSNTKALGKLYMQNNHLSFHDFSFVLQEQDIADTDGTPFQYLENLQILNLRNNSITTIFRDWNYNNLHLRELDLSYNNISTLSYLSLQMSASI

Target ORF information:

RefSeq Version	XM_001648474.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL014337-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001648474.1

ORF Insert Sequence:

ATGGCACGGG CGCTCCCATT ACTGGCACTG TTACTCACGC ACAATTTGGC AAGTTCATTT 
CCGCAGCACC ACTACACGGA CTACGTGCTG GGCGGAGAAC TGGTCAGTGG AGGAGGGGCT 
GGCGGAACCA CGACGGGAAT TGGATCCAGT GGCACAGGCG GGACCGGCAC CGGCGCCGGT 
GAACAACGAT TCACATGTCC CGAACCGACG ACCACCACTA GCGATTGCAC TTGCATGTAC 
GCCAGCTCCG ATTACGAAAT TCAATGTCCC GTGATTAATC CGGAAATTAC GGTGAAAATA 
AAACCTGGCA TCTACGCCCA GATACAGTGC TACGAGAAGC AGGAGTTTAG CGACCTGCCA 
ACGTTGAATG TTGGCAACAC GACCCAGGTC AAAATCATCC ATTGCCCGCT GCCACAGCGA 
CAGTCCATTA AGCAGTTCAT CAAATTCCTG GGAGTTAACC ACGTGAAGGA TTTCTGGTAT 
CAGAATTATG GGAAGGATTT CGGAGTGCGA TTGATCCGGC AGCATTTCGA GGGTATGCAT 
GATTTGGAAA AACTTTTCCT CAGCTCAGGA ATCGAAGACA TCCAACCGGA TCTGTTTGCC 
GATCTGCCGA ACCTGAAGTG GCTCATTCTA CGAAGCAATC ATGTCAAACT CCTGCACAAC 
GTTTTCGATA ACCTGACTAA CTTGATTATA CTGGAACTGG GAGCCAACCA GATCACCGAG 
CTTGAACCGG GTCTGTTCAA GAATCAACGG AAACTACGAC ATCTCAACTT GTGGCGCAAT 
CAGCTGCGTA ACATCTCCAA AGAATCTTTC CGCGGAGCGG AAACACTCCA AGAGCTAGAT 
CTCTCCGTCA ACGCGATCGA AACGCTCAAT TCCGATGTGT TCACGTTGCT TCCGGATCTC 
ACTGTCCTGA ATCTCGGGTT CAATCGATTC ACCGTTCTAC CCGATAATCT CCTTTCCGAA 
AACCGCAAGC TGCGGGAGTT CAAATTCATC AATAATCAGG CTCCGCTGCA AACCCTACCG 
GAAGCCTTTC TCGGCAACCT CCCTCAGCTG AAATCGGTCA TCTTGAATCG CTGCAGCTTC 
ACGCATCTGC CCGCATCGCT GTTCCGCGGA TCGAGCGAAA TCACCCACAT GGATCTGAGC 
TACAACCAGC TGGGATCCGT TCCGGAGTTG CTTCTCCGCG ATCAGCTGCG TCTTCAAGAT 
CTGAATTTGG CATACAACGA ACTGGAATCG CTTCCGGAAC ACTTGCTGGA GAACACCCGG 
GAGTTGCTCA CCCTGCGACT GTCGTACAAC CGGCTCTACA ATCTTTCGGC AAACATCTTC 
GCCTCGCTGG ACAAGCTCAC CGAACTGCAC CTGAACGACA ACTTCCTGCA CGCCATCGAT 
CGGCTCACCT TCAGCAACAC CAAAGCCCTC GGAAAGCTGT ACATGCAAAA CAACCACCTC 
TCCTTCCACG ATTTCAGTTT CGTCCTCCAG GAGCAGGACA TCGCCGACAC CGATGGCACA 
CCGTTCCAAT ACCTGGAAAA CCTTCAAATC CTAAACCTCC GCAACAACAG CATCACCACC 
ATCTTCCGTG ATTGGAACTA CAACAACCTG CATCTGCGCG AGCTCGATCT CAGCTACAAC 
AACATCTCCA CCCTGTCCTA TCTGAGCCTA CAGATGTCCG CGTCAATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5564186 ( XM_021853596.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709288.1 Aedes aegypti protein toll (LOC5564186), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAe130896
Clone ID Related Accession (Same CDS sequence)	XM_021853596.1 , XM_021853026.1
Accession Version	XM_021853596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	protein toll
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGGG CGCTCCCATT ACTGGCACTG TTACTCACGC ACAATTTGGC AAGTTCATTT 
CCGCAGCACC ACTACACGGA CTACGTGCTG GGCGGAGAAC TGGTCAGTGG AGGAGGGGCT 
GGCGGAACCA CGACGGGAAT TGGATCCAGT GGCACAGGCG GGACCGGCAC CGGCGCCGGT 
GAACAACGAT TCACATGTCC CGAACCGACG ACCACCACTA GCGATTGCAC TTGCATGTAC 
GCCAGCTCCG ATTACGAAAT TCAATGTCCC GTGATTAATC CGGAAATTAC GGTGAAAATA 
AAACCTGGCA TCTACGCCCA GATACAGTGC TACGAGAAGC AGGAGTTTAG CGACCTGCCA 
ACGTTGAATG TTGGCAACAC GACCCAGGTC AAAATCATCC ATTGCCCGCT GCCACAGCGA 
CAGTCCATTA AGCAGTTCAT CAAATTCCTG GGAGTTAACC ACGTGAAGGA TTTCTGGTAT 
CAGAATTATG GGAAGGATTT CGGAGTGCGA TTGATCCGGC AGCATTTCGA GGGTATGCAT 
GATTTGGAAA AACTTTTCCT CAGCTCAGGA ATCGAAGACA TCCAACCGGA TCTGTTTGCC 
GATCTGCCGA ACCTGAAGTG GCTCATTCTA CGAAGCAATC ATGTCAAACT CCTGCACAAC 
GTTTTCGATA ACCTGACTAA CTTGATTATA CTGGAACTGG GAGCCAACCA GATCACCGAG 
CTTGAACCGG GTCTGTTCAA GAATCAACGG AAACTACGAC ATCTCAACTT GTGGCGCAAT 
CAGCTGCGTA ACATCTCCAA AGAATCTTTC CGCGGAGCGG AAACACTCCA AGAGCTAGAT 
CTCTCCGTCA ACGCGATCGA AACGCTCAAT TCCGATGTGT TCACGTTGCT TCCGGATCTC 
ACTGTCCTGA ATCTCGGGTT CAATCGATTC ACCGTTCTAC CCGATAATCT CCTTTCCGAA 
AACCGCAAGC TGCGGGAGTT CAAATTCATC AATAATCAGG CTCCGCTGCA AACCCTACCG 
GAAGCCTTTC TCGGCAACCT CCCTCAGCTG AAATCGGTCA TCTTGAATCG CTGCAGCTTC 
ACGCATCTGC CCGCATCGCT GTTCCGCGGA TCGAGCGAAA TCACCCACAT GGATCTGAGC 
TACAACCAGC TGGGATCCGT TCCGGAGTTG CTTCTCCGCG ATCAGCTGCG TCTTCAAGAT 
CTGAATTTGG CATACAACGA ACTGGAATCG CTTCCGGAAC ACTTGCTGGA GAACACCCGG 
GAGTTGCTCA CCCTGCGACT GTCGTACAAC CGGCTCTACA ATCTTTCGGC AAACATCTTC 
GCCTCGCTGG ACAAGCTCAC CGAACTGCAC CTGAACGACA ACTTCCTGCA CGCCATCGAT 
CGGCTCACCT TCAGCAACAC CAAAGCCCTC GGAAAGCTGT ACATGCAAAA CAACCACCTC 
TCCTTCCACG ATTTCAGTTT CGTCCTCCAG GAGCAGGACA TCGCCGACAC CGATGGCACA 
CCGTTCCAAT ACCTGGAAAA CCTTCAAATC CTAAACCTCC GCAACAACAG CATCACCACC 
ATCTTCCGTG ATTGGAACTA CAACAACCTG CATCTGCGCG AGCTCGATCT CAGCTACAAC 
AACATCTCCA CCCTGTCCTA TCTGAGCCTA CAGTTCCTCT CGCAGGATGT CCGCGTCAAT 
CTAACGCACA ACCGAATATC CGAGATCGAT CTCAAAGACA TGGAGCCCAT CATCACCTCC 
CAGCGATCGC AGGACGACAG CAAGATCTAC GTCTACGTCA ACGACAACCC CCTGAACTGC 
AACTGCGTTA TCTTCTCGTT CGTGCAATAT CTCCTGAACG AGCTGGATAG CGCCGTCTAC 
CGACGGATCC AATTCGTAGC GGACGATCTT CGCTGTTCAG AACCACAGAA CCTAGAAGGC 
GTCCACGTAT CCAAACTGCA AACCAAAGAC CTCCTCTGTC AGCTGGATCA ACCTGGCACG 
GAAATCAAGC ATTGCCCAGC GAACTGCAGC TGCCACGTGC GGCCACTCGA TCGCGGAGTG 
ATCGTCAACT GCACGCGTCA AGGCCTAACG GAAATACCCG CGTTGCCGCA ACCCACCATG 
TTCGGATACA ACTTCATCGA GTTGCACGTG GAGGACAACA ACATTACCGA GTTGCCGACG 
GAGAACTTGA CTGGCTATGG CAAGGTCGCC GAGCTGTACG CCCGGAATAA TGCGATCGTT 
GAACTGCTCC CGGAGAATCT ACCGAGTAGC CTGAGGATAC TGGATGTGAC GCAGAACAAA 
TTCGTTATGC TAAATCAGTC GGTCGTCGAG TCGCTGAACA GCAGTAAGGT CCTGGAGAGC 
TTGCACTTGT CGGGAAATAA ATGGCGTTGC GATTGCAGCT CAGCGCAGTT CCTGAACTTC 
GTCCAGCAGA ATCACAAAAA AATAGCAGAT CTGGGTCAGA TAACGTGCGA AACCGGTGAA 
GAGTTCAGCT TGATCACGGT GAACAGCTTG TGCAACGAGA GCGTGACAAC GGTCATAACC 
GTCAGCGTGA TCTTGTCGAT ATTGGGACTG CTAATAGGGC TGTTCACGGT GCTGTACTTT 
GTGTACCACA TGGAGATCAA GGTGTGGCTG TTCACGCACA ACATGTGCCT GTGGTGGGTC 
TCGGAAGAGG AACTGGACAA GGACAAGCTG TACGATGCCT TCATATCGTA CTCGCACAAG 
GACGAAGAGT TCATCACGGA GCACCTGGTG CCGACCTTGG AGAAGGAACC GATGAACTTC 
AAGACGTGCT GGCATGTGCG CGACTGGATG CCCGGAGAGC TGATTCCGAC GCAGATCGCA 
AAATCCGTGG AGGACTCCCG GCGAACCATC GTCGTCCTGT CGACCAACTT CCTCGAGTCG 
GTTTGGGGTC GCATGGAGTT CCGCACGGCC CACCTCACCT CGATGGAGGA GAAGCGCGCT 
CGGGTGATCG TCATCATCTA CGGAGACATC GGCAGCATCG AAGATCTGGA CCCGGAGCTG 
AAGGCGTACC TCAAGATGAA CACCTACGTC AAGTGGGGTG ATCAATGGTT CTGGGACAAA 
CTGCGCTACG CGATGCCTCA TCCTCCGACC GTCAAAGGAC TCAAGGGCAA GGGTTTGATC 
AAGAACCACC TGAAGAGCTC CGTCGACGAT AAGCTGGAGT TGATCAAGCC CGTTCCGGTT 
ACGCCGCCGC CACTGACGAC TCCTCCAGCC GAAATGAACG GACACGCCAG ATCACCTCCG 
TTCACCATCT CCAACGGAAA GGTCGTCAAC GGCAACGGTT ACCACATCAA TGGACACGTG 
ATGAACGGTC ACGTCAACGG ACACATCAAC GGAGCATTCG TTATCAACAG CAACGCCAAA 
CAGAGCGACG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709288.1
CDS	1756..5130
Translation	MARALPLLALLLTHNLASSFPQHHYTDYVLGGELVSGGGAGGTTTGIGSSGTGGTGTGAGEQRFTCPEPTTTTSDCTCMYASSDYEIQCPVINPEITVKIKPGIYAQIQCYEKQEFSDLPTLNVGNTTQVKIIHCPLPQRQSIKQFIKFLGVNHVKDFWYQNYGKDFGVRLIRQHFEGMHDLEKLFLSSGIEDIQPDLFADLPNLKWLILRSNHVKLLHNVFDNLTNLIILELGANQITELEPGLFKNQRKLRHLNLWRNQLRNISKESFRGAETLQELDLSVNAIETLNSDVFTLLPDLTVLNLGFNRFTVLPDNLLSENRKLREFKFINNQAPLQTLPEAFLGNLPQLKSVILNRCSFTHLPASLFRGSSEITHMDLSYNQLGSVPELLLRDQLRLQDLNLAYNELESLPEHLLENTRELLTLRLSYNRLYNLSANIFASLDKLTELHLNDNFLHAIDRLTFSNTKALGKLYMQNNHLSFHDFSFVLQEQDIADTDGTPFQYLENLQILNLRNNSITTIFRDWNYNNLHLRELDLSYNNISTLSYLSLQFLSQDVRVNLTHNRISEIDLKDMEPIITSQRSQDDSKIYVYVNDNPLNCNCVIFSFVQYLLNELDSAVYRRIQFVADDLRCSEPQNLEGVHVSKLQTKDLLCQLDQPGTEIKHCPANCSCHVRPLDRGVIVNCTRQGLTEIPALPQPTMFGYNFIELHVEDNNITELPTENLTGYGKVAELYARNNAIVELLPENLPSSLRILDVTQNKFVMLNQSVVESLNSSKVLESLHLSGNKWRCDCSSAQFLNFVQQNHKKIADLGQITCETGEEFSLITVNSLCNESVTTVITVSVILSILGLLIGLFTVLYFVYHMEIKVWLFTHNMCLWWVSEEELDKDKLYDAFISYSHKDEEFITEHLVPTLEKEPMNFKTCWHVRDWMPGELIPTQIAKSVEDSRRTIVVLSTNFLESVWGRMEFRTAHLTSMEEKRARVIVIIYGDIGSIEDLDPELKAYLKMNTYVKWGDQWFWDKLRYAMPHPPTVKGLKGKGLIKNHLKSSVDDKLELIKPVPVTPPPLTTPPAEMNGHARSPPFTISNGKVVNGNGYHINGHVMNGHVNGHINGAFVINSNAKQSDV

Target ORF information:

RefSeq Version	XM_021853596.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti protein toll (LOC5564186), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021853596.1

ORF Insert Sequence:

ATGGCACGGG CGCTCCCATT ACTGGCACTG TTACTCACGC ACAATTTGGC AAGTTCATTT 
CCGCAGCACC ACTACACGGA CTACGTGCTG GGCGGAGAAC TGGTCAGTGG AGGAGGGGCT 
GGCGGAACCA CGACGGGAAT TGGATCCAGT GGCACAGGCG GGACCGGCAC CGGCGCCGGT 
GAACAACGAT TCACATGTCC CGAACCGACG ACCACCACTA GCGATTGCAC TTGCATGTAC 
GCCAGCTCCG ATTACGAAAT TCAATGTCCC GTGATTAATC CGGAAATTAC GGTGAAAATA 
AAACCTGGCA TCTACGCCCA GATACAGTGC TACGAGAAGC AGGAGTTTAG CGACCTGCCA 
ACGTTGAATG TTGGCAACAC GACCCAGGTC AAAATCATCC ATTGCCCGCT GCCACAGCGA 
CAGTCCATTA AGCAGTTCAT CAAATTCCTG GGAGTTAACC ACGTGAAGGA TTTCTGGTAT 
CAGAATTATG GGAAGGATTT CGGAGTGCGA TTGATCCGGC AGCATTTCGA GGGTATGCAT 
GATTTGGAAA AACTTTTCCT CAGCTCAGGA ATCGAAGACA TCCAACCGGA TCTGTTTGCC 
GATCTGCCGA ACCTGAAGTG GCTCATTCTA CGAAGCAATC ATGTCAAACT CCTGCACAAC 
GTTTTCGATA ACCTGACTAA CTTGATTATA CTGGAACTGG GAGCCAACCA GATCACCGAG 
CTTGAACCGG GTCTGTTCAA GAATCAACGG AAACTACGAC ATCTCAACTT GTGGCGCAAT 
CAGCTGCGTA ACATCTCCAA AGAATCTTTC CGCGGAGCGG AAACACTCCA AGAGCTAGAT 
CTCTCCGTCA ACGCGATCGA AACGCTCAAT TCCGATGTGT TCACGTTGCT TCCGGATCTC 
ACTGTCCTGA ATCTCGGGTT CAATCGATTC ACCGTTCTAC CCGATAATCT CCTTTCCGAA 
AACCGCAAGC TGCGGGAGTT CAAATTCATC AATAATCAGG CTCCGCTGCA AACCCTACCG 
GAAGCCTTTC TCGGCAACCT CCCTCAGCTG AAATCGGTCA TCTTGAATCG CTGCAGCTTC 
ACGCATCTGC CCGCATCGCT GTTCCGCGGA TCGAGCGAAA TCACCCACAT GGATCTGAGC 
TACAACCAGC TGGGATCCGT TCCGGAGTTG CTTCTCCGCG ATCAGCTGCG TCTTCAAGAT 
CTGAATTTGG CATACAACGA ACTGGAATCG CTTCCGGAAC ACTTGCTGGA GAACACCCGG 
GAGTTGCTCA CCCTGCGACT GTCGTACAAC CGGCTCTACA ATCTTTCGGC AAACATCTTC 
GCCTCGCTGG ACAAGCTCAC CGAACTGCAC CTGAACGACA ACTTCCTGCA CGCCATCGAT 
CGGCTCACCT TCAGCAACAC CAAAGCCCTC GGAAAGCTGT ACATGCAAAA CAACCACCTC 
TCCTTCCACG ATTTCAGTTT CGTCCTCCAG GAGCAGGACA TCGCCGACAC CGATGGCACA 
CCGTTCCAAT ACCTGGAAAA CCTTCAAATC CTAAACCTCC GCAACAACAG CATCACCACC 
ATCTTCCGTG ATTGGAACTA CAACAACCTG CATCTGCGCG AGCTCGATCT CAGCTACAAC 
AACATCTCCA CCCTGTCCTA TCTGAGCCTA CAGTTCCTCT CGCAGGATGT CCGCGTCAAT 
CTAACGCACA ACCGAATATC CGAGATCGAT CTCAAAGACA TGGAGCCCAT CATCACCTCC 
CAGCGATCGC AGGACGACAG CAAGATCTAC GTCTACGTCA ACGACAACCC CCTGAACTGC 
AACTGCGTTA TCTTCTCGTT CGTGCAATAT CTCCTGAACG AGCTGGATAG CGCCGTCTAC 
CGACGGATCC AATTCGTAGC GGACGATCTT CGCTGTTCAG AACCACAGAA CCTAGAAGGC 
GTCCACGTAT CCAAACTGCA AACCAAAGAC CTCCTCTGTC AGCTGGATCA ACCTGGCACG 
GAAATCAAGC ATTGCCCAGC GAACTGCAGC TGCCACGTGC GGCCACTCGA TCGCGGAGTG 
ATCGTCAACT GCACGCGTCA AGGCCTAACG GAAATACCCG CGTTGCCGCA ACCCACCATG 
TTCGGATACA ACTTCATCGA GTTGCACGTG GAGGACAACA ACATTACCGA GTTGCCGACG 
GAGAACTTGA CTGGCTATGG CAAGGTCGCC GAGCTGTACG CCCGGAATAA TGCGATCGTT 
GAACTGCTCC CGGAGAATCT ACCGAGTAGC CTGAGGATAC TGGATGTGAC GCAGAACAAA 
TTCGTTATGC TAAATCAGTC GGTCGTCGAG TCGCTGAACA GCAGTAAGGT CCTGGAGAGC 
TTGCACTTGT CGGGAAATAA ATGGCGTTGC GATTGCAGCT CAGCGCAGTT CCTGAACTTC 
GTCCAGCAGA ATCACAAAAA AATAGCAGAT CTGGGTCAGA TAACGTGCGA AACCGGTGAA 
GAGTTCAGCT TGATCACGGT GAACAGCTTG TGCAACGAGA GCGTGACAAC GGTCATAACC 
GTCAGCGTGA TCTTGTCGAT ATTGGGACTG CTAATAGGGC TGTTCACGGT GCTGTACTTT 
GTGTACCACA TGGAGATCAA GGTGTGGCTG TTCACGCACA ACATGTGCCT GTGGTGGGTC 
TCGGAAGAGG AACTGGACAA GGACAAGCTG TACGATGCCT TCATATCGTA CTCGCACAAG 
GACGAAGAGT TCATCACGGA GCACCTGGTG CCGACCTTGG AGAAGGAACC GATGAACTTC 
AAGACGTGCT GGCATGTGCG CGACTGGATG CCCGGAGAGC TGATTCCGAC GCAGATCGCA 
AAATCCGTGG AGGACTCCCG GCGAACCATC GTCGTCCTGT CGACCAACTT CCTCGAGTCG 
GTTTGGGGTC GCATGGAGTT CCGCACGGCC CACCTCACCT CGATGGAGGA GAAGCGCGCT 
CGGGTGATCG TCATCATCTA CGGAGACATC GGCAGCATCG AAGATCTGGA CCCGGAGCTG 
AAGGCGTACC TCAAGATGAA CACCTACGTC AAGTGGGGTG ATCAATGGTT CTGGGACAAA 
CTGCGCTACG CGATGCCTCA TCCTCCGACC GTCAAAGGAC TCAAGGGCAA GGGTTTGATC 
AAGAACCACC TGAAGAGCTC CGTCGACGAT AAGCTGGAGT TGATCAAGCC CGTTCCGGTT 
ACGCCGCCGC CACTGACGAC TCCTCCAGCC GAAATGAACG GACACGCCAG ATCACCTCCG 
TTCACCATCT CCAACGGAAA GGTCGTCAAC GGCAACGGTT ACCACATCAA TGGACACGTG 
ATGAACGGTC ACGTCAACGG ACACATCAAC GGAGCATTCG TTATCAACAG CAACGCCAAA 
CAGAGCGACG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5564186 ( XM_021853026.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021708718.1 Aedes aegypti protein toll (LOC5564186), transcript variant X1, mRNA.

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CloneID	OAe130896
Clone ID Related Accession (Same CDS sequence)	XM_021853596.1 , XM_021853026.1
Accession Version	XM_021853026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	protein toll
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGGG CGCTCCCATT ACTGGCACTG TTACTCACGC ACAATTTGGC AAGTTCATTT 
CCGCAGCACC ACTACACGGA CTACGTGCTG GGCGGAGAAC TGGTCAGTGG AGGAGGGGCT 
GGCGGAACCA CGACGGGAAT TGGATCCAGT GGCACAGGCG GGACCGGCAC CGGCGCCGGT 
GAACAACGAT TCACATGTCC CGAACCGACG ACCACCACTA GCGATTGCAC TTGCATGTAC 
GCCAGCTCCG ATTACGAAAT TCAATGTCCC GTGATTAATC CGGAAATTAC GGTGAAAATA 
AAACCTGGCA TCTACGCCCA GATACAGTGC TACGAGAAGC AGGAGTTTAG CGACCTGCCA 
ACGTTGAATG TTGGCAACAC GACCCAGGTC AAAATCATCC ATTGCCCGCT GCCACAGCGA 
CAGTCCATTA AGCAGTTCAT CAAATTCCTG GGAGTTAACC ACGTGAAGGA TTTCTGGTAT 
CAGAATTATG GGAAGGATTT CGGAGTGCGA TTGATCCGGC AGCATTTCGA GGGTATGCAT 
GATTTGGAAA AACTTTTCCT CAGCTCAGGA ATCGAAGACA TCCAACCGGA TCTGTTTGCC 
GATCTGCCGA ACCTGAAGTG GCTCATTCTA CGAAGCAATC ATGTCAAACT CCTGCACAAC 
GTTTTCGATA ACCTGACTAA CTTGATTATA CTGGAACTGG GAGCCAACCA GATCACCGAG 
CTTGAACCGG GTCTGTTCAA GAATCAACGG AAACTACGAC ATCTCAACTT GTGGCGCAAT 
CAGCTGCGTA ACATCTCCAA AGAATCTTTC CGCGGAGCGG AAACACTCCA AGAGCTAGAT 
CTCTCCGTCA ACGCGATCGA AACGCTCAAT TCCGATGTGT TCACGTTGCT TCCGGATCTC 
ACTGTCCTGA ATCTCGGGTT CAATCGATTC ACCGTTCTAC CCGATAATCT CCTTTCCGAA 
AACCGCAAGC TGCGGGAGTT CAAATTCATC AATAATCAGG CTCCGCTGCA AACCCTACCG 
GAAGCCTTTC TCGGCAACCT CCCTCAGCTG AAATCGGTCA TCTTGAATCG CTGCAGCTTC 
ACGCATCTGC CCGCATCGCT GTTCCGCGGA TCGAGCGAAA TCACCCACAT GGATCTGAGC 
TACAACCAGC TGGGATCCGT TCCGGAGTTG CTTCTCCGCG ATCAGCTGCG TCTTCAAGAT 
CTGAATTTGG CATACAACGA ACTGGAATCG CTTCCGGAAC ACTTGCTGGA GAACACCCGG 
GAGTTGCTCA CCCTGCGACT GTCGTACAAC CGGCTCTACA ATCTTTCGGC AAACATCTTC 
GCCTCGCTGG ACAAGCTCAC CGAACTGCAC CTGAACGACA ACTTCCTGCA CGCCATCGAT 
CGGCTCACCT TCAGCAACAC CAAAGCCCTC GGAAAGCTGT ACATGCAAAA CAACCACCTC 
TCCTTCCACG ATTTCAGTTT CGTCCTCCAG GAGCAGGACA TCGCCGACAC CGATGGCACA 
CCGTTCCAAT ACCTGGAAAA CCTTCAAATC CTAAACCTCC GCAACAACAG CATCACCACC 
ATCTTCCGTG ATTGGAACTA CAACAACCTG CATCTGCGCG AGCTCGATCT CAGCTACAAC 
AACATCTCCA CCCTGTCCTA TCTGAGCCTA CAGTTCCTCT CGCAGGATGT CCGCGTCAAT 
CTAACGCACA ACCGAATATC CGAGATCGAT CTCAAAGACA TGGAGCCCAT CATCACCTCC 
CAGCGATCGC AGGACGACAG CAAGATCTAC GTCTACGTCA ACGACAACCC CCTGAACTGC 
AACTGCGTTA TCTTCTCGTT CGTGCAATAT CTCCTGAACG AGCTGGATAG CGCCGTCTAC 
CGACGGATCC AATTCGTAGC GGACGATCTT CGCTGTTCAG AACCACAGAA CCTAGAAGGC 
GTCCACGTAT CCAAACTGCA AACCAAAGAC CTCCTCTGTC AGCTGGATCA ACCTGGCACG 
GAAATCAAGC ATTGCCCAGC GAACTGCAGC TGCCACGTGC GGCCACTCGA TCGCGGAGTG 
ATCGTCAACT GCACGCGTCA AGGCCTAACG GAAATACCCG CGTTGCCGCA ACCCACCATG 
TTCGGATACA ACTTCATCGA GTTGCACGTG GAGGACAACA ACATTACCGA GTTGCCGACG 
GAGAACTTGA CTGGCTATGG CAAGGTCGCC GAGCTGTACG CCCGGAATAA TGCGATCGTT 
GAACTGCTCC CGGAGAATCT ACCGAGTAGC CTGAGGATAC TGGATGTGAC GCAGAACAAA 
TTCGTTATGC TAAATCAGTC GGTCGTCGAG TCGCTGAACA GCAGTAAGGT CCTGGAGAGC 
TTGCACTTGT CGGGAAATAA ATGGCGTTGC GATTGCAGCT CAGCGCAGTT CCTGAACTTC 
GTCCAGCAGA ATCACAAAAA AATAGCAGAT CTGGGTCAGA TAACGTGCGA AACCGGTGAA 
GAGTTCAGCT TGATCACGGT GAACAGCTTG TGCAACGAGA GCGTGACAAC GGTCATAACC 
GTCAGCGTGA TCTTGTCGAT ATTGGGACTG CTAATAGGGC TGTTCACGGT GCTGTACTTT 
GTGTACCACA TGGAGATCAA GGTGTGGCTG TTCACGCACA ACATGTGCCT GTGGTGGGTC 
TCGGAAGAGG AACTGGACAA GGACAAGCTG TACGATGCCT TCATATCGTA CTCGCACAAG 
GACGAAGAGT TCATCACGGA GCACCTGGTG CCGACCTTGG AGAAGGAACC GATGAACTTC 
AAGACGTGCT GGCATGTGCG CGACTGGATG CCCGGAGAGC TGATTCCGAC GCAGATCGCA 
AAATCCGTGG AGGACTCCCG GCGAACCATC GTCGTCCTGT CGACCAACTT CCTCGAGTCG 
GTTTGGGGTC GCATGGAGTT CCGCACGGCC CACCTCACCT CGATGGAGGA GAAGCGCGCT 
CGGGTGATCG TCATCATCTA CGGAGACATC GGCAGCATCG AAGATCTGGA CCCGGAGCTG 
AAGGCGTACC TCAAGATGAA CACCTACGTC AAGTGGGGTG ATCAATGGTT CTGGGACAAA 
CTGCGCTACG CGATGCCTCA TCCTCCGACC GTCAAAGGAC TCAAGGGCAA GGGTTTGATC 
AAGAACCACC TGAAGAGCTC CGTCGACGAT AAGCTGGAGT TGATCAAGCC CGTTCCGGTT 
ACGCCGCCGC CACTGACGAC TCCTCCAGCC GAAATGAACG GACACGCCAG ATCACCTCCG 
TTCACCATCT CCAACGGAAA GGTCGTCAAC GGCAACGGTT ACCACATCAA TGGACACGTG 
ATGAACGGTC ACGTCAACGG ACACATCAAC GGAGCATTCG TTATCAACAG CAACGCCAAA 
CAGAGCGACG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021708718.1
CDS	1796..5170
Translation	MARALPLLALLLTHNLASSFPQHHYTDYVLGGELVSGGGAGGTTTGIGSSGTGGTGTGAGEQRFTCPEPTTTTSDCTCMYASSDYEIQCPVINPEITVKIKPGIYAQIQCYEKQEFSDLPTLNVGNTTQVKIIHCPLPQRQSIKQFIKFLGVNHVKDFWYQNYGKDFGVRLIRQHFEGMHDLEKLFLSSGIEDIQPDLFADLPNLKWLILRSNHVKLLHNVFDNLTNLIILELGANQITELEPGLFKNQRKLRHLNLWRNQLRNISKESFRGAETLQELDLSVNAIETLNSDVFTLLPDLTVLNLGFNRFTVLPDNLLSENRKLREFKFINNQAPLQTLPEAFLGNLPQLKSVILNRCSFTHLPASLFRGSSEITHMDLSYNQLGSVPELLLRDQLRLQDLNLAYNELESLPEHLLENTRELLTLRLSYNRLYNLSANIFASLDKLTELHLNDNFLHAIDRLTFSNTKALGKLYMQNNHLSFHDFSFVLQEQDIADTDGTPFQYLENLQILNLRNNSITTIFRDWNYNNLHLRELDLSYNNISTLSYLSLQFLSQDVRVNLTHNRISEIDLKDMEPIITSQRSQDDSKIYVYVNDNPLNCNCVIFSFVQYLLNELDSAVYRRIQFVADDLRCSEPQNLEGVHVSKLQTKDLLCQLDQPGTEIKHCPANCSCHVRPLDRGVIVNCTRQGLTEIPALPQPTMFGYNFIELHVEDNNITELPTENLTGYGKVAELYARNNAIVELLPENLPSSLRILDVTQNKFVMLNQSVVESLNSSKVLESLHLSGNKWRCDCSSAQFLNFVQQNHKKIADLGQITCETGEEFSLITVNSLCNESVTTVITVSVILSILGLLIGLFTVLYFVYHMEIKVWLFTHNMCLWWVSEEELDKDKLYDAFISYSHKDEEFITEHLVPTLEKEPMNFKTCWHVRDWMPGELIPTQIAKSVEDSRRTIVVLSTNFLESVWGRMEFRTAHLTSMEEKRARVIVIIYGDIGSIEDLDPELKAYLKMNTYVKWGDQWFWDKLRYAMPHPPTVKGLKGKGLIKNHLKSSVDDKLELIKPVPVTPPPLTTPPAEMNGHARSPPFTISNGKVVNGNGYHINGHVMNGHVNGHINGAFVINSNAKQSDV

Target ORF information:

RefSeq Version	XM_021853026.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti protein toll (LOC5564186), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021853026.1

ORF Insert Sequence:

ATGGCACGGG CGCTCCCATT ACTGGCACTG TTACTCACGC ACAATTTGGC AAGTTCATTT 
CCGCAGCACC ACTACACGGA CTACGTGCTG GGCGGAGAAC TGGTCAGTGG AGGAGGGGCT 
GGCGGAACCA CGACGGGAAT TGGATCCAGT GGCACAGGCG GGACCGGCAC CGGCGCCGGT 
GAACAACGAT TCACATGTCC CGAACCGACG ACCACCACTA GCGATTGCAC TTGCATGTAC 
GCCAGCTCCG ATTACGAAAT TCAATGTCCC GTGATTAATC CGGAAATTAC GGTGAAAATA 
AAACCTGGCA TCTACGCCCA GATACAGTGC TACGAGAAGC AGGAGTTTAG CGACCTGCCA 
ACGTTGAATG TTGGCAACAC GACCCAGGTC AAAATCATCC ATTGCCCGCT GCCACAGCGA 
CAGTCCATTA AGCAGTTCAT CAAATTCCTG GGAGTTAACC ACGTGAAGGA TTTCTGGTAT 
CAGAATTATG GGAAGGATTT CGGAGTGCGA TTGATCCGGC AGCATTTCGA GGGTATGCAT 
GATTTGGAAA AACTTTTCCT CAGCTCAGGA ATCGAAGACA TCCAACCGGA TCTGTTTGCC 
GATCTGCCGA ACCTGAAGTG GCTCATTCTA CGAAGCAATC ATGTCAAACT CCTGCACAAC 
GTTTTCGATA ACCTGACTAA CTTGATTATA CTGGAACTGG GAGCCAACCA GATCACCGAG 
CTTGAACCGG GTCTGTTCAA GAATCAACGG AAACTACGAC ATCTCAACTT GTGGCGCAAT 
CAGCTGCGTA ACATCTCCAA AGAATCTTTC CGCGGAGCGG AAACACTCCA AGAGCTAGAT 
CTCTCCGTCA ACGCGATCGA AACGCTCAAT TCCGATGTGT TCACGTTGCT TCCGGATCTC 
ACTGTCCTGA ATCTCGGGTT CAATCGATTC ACCGTTCTAC CCGATAATCT CCTTTCCGAA 
AACCGCAAGC TGCGGGAGTT CAAATTCATC AATAATCAGG CTCCGCTGCA AACCCTACCG 
GAAGCCTTTC TCGGCAACCT CCCTCAGCTG AAATCGGTCA TCTTGAATCG CTGCAGCTTC 
ACGCATCTGC CCGCATCGCT GTTCCGCGGA TCGAGCGAAA TCACCCACAT GGATCTGAGC 
TACAACCAGC TGGGATCCGT TCCGGAGTTG CTTCTCCGCG ATCAGCTGCG TCTTCAAGAT 
CTGAATTTGG CATACAACGA ACTGGAATCG CTTCCGGAAC ACTTGCTGGA GAACACCCGG 
GAGTTGCTCA CCCTGCGACT GTCGTACAAC CGGCTCTACA ATCTTTCGGC AAACATCTTC 
GCCTCGCTGG ACAAGCTCAC CGAACTGCAC CTGAACGACA ACTTCCTGCA CGCCATCGAT 
CGGCTCACCT TCAGCAACAC CAAAGCCCTC GGAAAGCTGT ACATGCAAAA CAACCACCTC 
TCCTTCCACG ATTTCAGTTT CGTCCTCCAG GAGCAGGACA TCGCCGACAC CGATGGCACA 
CCGTTCCAAT ACCTGGAAAA CCTTCAAATC CTAAACCTCC GCAACAACAG CATCACCACC 
ATCTTCCGTG ATTGGAACTA CAACAACCTG CATCTGCGCG AGCTCGATCT CAGCTACAAC 
AACATCTCCA CCCTGTCCTA TCTGAGCCTA CAGTTCCTCT CGCAGGATGT CCGCGTCAAT 
CTAACGCACA ACCGAATATC CGAGATCGAT CTCAAAGACA TGGAGCCCAT CATCACCTCC 
CAGCGATCGC AGGACGACAG CAAGATCTAC GTCTACGTCA ACGACAACCC CCTGAACTGC 
AACTGCGTTA TCTTCTCGTT CGTGCAATAT CTCCTGAACG AGCTGGATAG CGCCGTCTAC 
CGACGGATCC AATTCGTAGC GGACGATCTT CGCTGTTCAG AACCACAGAA CCTAGAAGGC 
GTCCACGTAT CCAAACTGCA AACCAAAGAC CTCCTCTGTC AGCTGGATCA ACCTGGCACG 
GAAATCAAGC ATTGCCCAGC GAACTGCAGC TGCCACGTGC GGCCACTCGA TCGCGGAGTG 
ATCGTCAACT GCACGCGTCA AGGCCTAACG GAAATACCCG CGTTGCCGCA ACCCACCATG 
TTCGGATACA ACTTCATCGA GTTGCACGTG GAGGACAACA ACATTACCGA GTTGCCGACG 
GAGAACTTGA CTGGCTATGG CAAGGTCGCC GAGCTGTACG CCCGGAATAA TGCGATCGTT 
GAACTGCTCC CGGAGAATCT ACCGAGTAGC CTGAGGATAC TGGATGTGAC GCAGAACAAA 
TTCGTTATGC TAAATCAGTC GGTCGTCGAG TCGCTGAACA GCAGTAAGGT CCTGGAGAGC 
TTGCACTTGT CGGGAAATAA ATGGCGTTGC GATTGCAGCT CAGCGCAGTT CCTGAACTTC 
GTCCAGCAGA ATCACAAAAA AATAGCAGAT CTGGGTCAGA TAACGTGCGA AACCGGTGAA 
GAGTTCAGCT TGATCACGGT GAACAGCTTG TGCAACGAGA GCGTGACAAC GGTCATAACC 
GTCAGCGTGA TCTTGTCGAT ATTGGGACTG CTAATAGGGC TGTTCACGGT GCTGTACTTT 
GTGTACCACA TGGAGATCAA GGTGTGGCTG TTCACGCACA ACATGTGCCT GTGGTGGGTC 
TCGGAAGAGG AACTGGACAA GGACAAGCTG TACGATGCCT TCATATCGTA CTCGCACAAG 
GACGAAGAGT TCATCACGGA GCACCTGGTG CCGACCTTGG AGAAGGAACC GATGAACTTC 
AAGACGTGCT GGCATGTGCG CGACTGGATG CCCGGAGAGC TGATTCCGAC GCAGATCGCA 
AAATCCGTGG AGGACTCCCG GCGAACCATC GTCGTCCTGT CGACCAACTT CCTCGAGTCG 
GTTTGGGGTC GCATGGAGTT CCGCACGGCC CACCTCACCT CGATGGAGGA GAAGCGCGCT 
CGGGTGATCG TCATCATCTA CGGAGACATC GGCAGCATCG AAGATCTGGA CCCGGAGCTG 
AAGGCGTACC TCAAGATGAA CACCTACGTC AAGTGGGGTG ATCAATGGTT CTGGGACAAA 
CTGCGCTACG CGATGCCTCA TCCTCCGACC GTCAAAGGAC TCAAGGGCAA GGGTTTGATC 
AAGAACCACC TGAAGAGCTC CGTCGACGAT AAGCTGGAGT TGATCAAGCC CGTTCCGGTT 
ACGCCGCCGC CACTGACGAC TCCTCCAGCC GAAATGAACG GACACGCCAG ATCACCTCCG 
TTCACCATCT CCAACGGAAA GGTCGTCAAC GGCAACGGTT ACCACATCAA TGGACACGTG 
ATGAACGGTC ACGTCAACGG ACACATCAAC GGAGCATTCG TTATCAACAG CAACGCCAAA 
CAGAGCGACG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5564186 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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