LOC23687986 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC23687986
Entrez Gene ID	23687986
Full Name	DNA ligase 1
Synonyms	AAEL017566
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011495273.1	XP_011493575.1	AAEL017566-PA
XM_021839925.1	XP_021695617.1	DNA ligase 1 isoform X1
XM_011495273.2	XP_011493575.2	DNA ligase 1 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC23687986 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC23687986 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe131374	XM_021839925.1 Latest version!	Aedes aegypti DNA ligase 1 (LOC23687986), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAe131375	XM_011495273.2 Latest version!	Aedes aegypti DNA ligase 1 (LOC23687986), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAe15211	XM_011495273.1 Latest version!	Aedes aegypti AAEL017566-RA mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC23687986 ( XM_021839925.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021695617.1 Aedes aegypti DNA ligase 1 (LOC23687986), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe131374
Clone ID Related Accession (Same CDS sequence)	XM_021839925.1
Accession Version	XM_021839925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	DNA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGAA CTACGATACT TTCCGTTCTG AATGTCACGA CGCAGTGTTC CGTTCGTAAT 
CTGCGAACGG TGCATCTGGC GCGTGTGGCA TATCCCGTGT TGTACCCTCT GAGTGGCCAA 
GGTACTAACA GTTATCCGTT GTGTTGTTCC GTTCTTCGTT TCTACGCCAC CAAACGTAGC 
AAAAAGGCAT CGACCGTTAA ATCCGAAGAT GAGGACAGTC CGATGAAGCG TTCCGGAGGG 
ACTGGACGGA AGTCGGGTGC CGTGTTCGAT TCGTCTCCGG AGGCGTCCCC GGAGAAGAAA 
TCGGTCGATG CGAAGACGAA CGGAGGTGGG AGTGTCCCCA AGCGAAGGCG CATTGCCTCG 
TCCAGTGAGG AGGAAGCAGG AGGATCGCCC AAGAAGTCCA GTCCAAAGAA AGTGGATGAG 
GAGAACCCCA TCAAGAAGGA GAAGCCTAGT CCGAAAGTGA AACCCAAGTC GGAATCGAAA 
CCTAAAAAAG ACGAAAAGAA ATCCCCCAAA TCTTCGGCGA AGAAGAGCTC CCCGAAAGAG 
AAGAAAGCGG TAAAAGAGGA GAAGCCGACT GAGAGCAAGG ACAAGGAAGT GAAGGAAGTT 
AAGAAGGAAG CCGAATCGCC CAAAGACAAA GAAGTGAAGG ATGTCAAGAG GGAAGCTGAA 
TCGCCCAAGA AGGACACTAA ACCGGTGGAC GTGAAGGACA AAACTGCGAA CGTGATGAGT 
TTCTTCACCA GTGCCGGAAA GAAAGATACC GGTTCCAGCA GCGGTTCCAA AGTGGATGGC 
GCCGATTACA ATCCGGGCAA GAAAAACTAC CATCCGATCA ACGACGCCTT CTGGAAGAAG 
GGTGACAAAG TACCGTACTT GGCTTTGGCC AGGACTTTCC AGATAATCGA AGAAACCAGC 
GGGCGGTTGC GGATGATAGA GATTCTGAGT AACTACTTCC GATCGGTGAT TTTGCTCAGT 
CCGAGCGATT TGCTGGCGAG CGTCTATCTC AGTCTGAATC AGTTGGCCCC GGCTTACGAG 
GGCGTTGAGC TGGGTATTGC GGAGCATACG CTGATGAAGG CCATTGCCCA GAGTACGGGA 
CGAAGTCTGG CCCAGATCAA GACCGATGCT CAGAATACGG GAGACTTGGG ACTGGTGGCC 
GAGCAGTCGA AGAGTAGTCA ACGGATGATG TTCCGTCCGG CGCCCCATTC GGTGGAAGGC 
GTCTTTGGGA AGCTTAAGGA AATCGCGAAG ATGACCGGAG CCGCTTCCAT GGCCAAGAAG 
GTCGACAAGA TCCAATCGAT GTTTGTTGCA TGCCGTCACT CGGAAGCTCG TTTCATCATT 
CGCTCGCTGG CCGGGAAGCT GCGGATAGGT CTGGCGGAGC AGTCTTTACT GCAGGCGTTG 
GCTCAAGCAT GTGCCATGAC TCCACCCAAC CAGAAAGATT GCAAAGAACC CATAGTGAAC 
GCGCTGAGCA AGTGCAGTGA AGCAACGGCC AAGGCCAAAG TCGACGAAAT CGCCCTGATC 
CTGAAAACGG TGTACTGTCA GTGCCCGAAC TACAACCAAA TTGTCCCGGT GCTGCTGGAA 
CACGGCATTG ATCACTTGCT AGAGAAATGC CCAATGATGC CGGGAACTCC TCTCAAACCT 
ATGTTGGCCC ATCCCACGAA AGGTGTTCAG GAAGTTCTGG AGCGATTCGA TGGAATCGAC 
TTCACCTGCG AGTGGAAATA CGACGGGGAA CGAGCTCAGA TCCATCTGCT GGAGGATGGC 
ACCGTTAACA TCTATAGCCG TAATCAGGAG AACAACACCA GCAAGTATCC GGACATCATC 
GCGCGGTTAG GCAACACCCG CAAGGAATCT GTAACCACCG CCATCCTGGA TTGCGAAGCC 
GTAGCTTGGG ATCCGGAGAA GAAGCAAATC CTTCCCTTCC AAATCCTCAG CACCCGGAAG 
CGAAAGGACG CCAACGAAGC GGACATCAAG GTCCAGGTGT GCGTCTACAT GTTCGATCTG 
CTGTATCTGA ACGGTAAGCC CCTCGTCGAG CGGCCATTCC TGGAGCGACG AGAACTAATG 
TACAAGCACT TCATCGAGTT CGAAGGCGAG TGGAAGTACG CTACCCGTCT GGACACCGGC 
GACATTGACG AACTGCAGCG CTTCCTGGAC GAAGCCGTCA AGGGCAACTG CGAAGGACTC 
ATGGTCAAAA CCCTCCAGAA GGAAGCCACC TACGAAATCG CCAAACGCTC CCGCAATTGG 
CTCAAGCTCA AAAAGGATTA CCTCTCGGGG GTGGGAGATT CTCTGGATCT GGTCGTAATC 
GGAGGCTACA AGGGCAGGGG CAAGCGAACC GGCACCTACG GAGGTTTCCT CCTTGCTTGC 
TACGACGAAG ACAACGAAGA GTATCAGAGC ATCTGCAAAA TTGGAACCGG ATTCTCCGAC 
GAGGACCTCC AAACGCACAC GGACTTCTTC AAGGACAAAA TCATACCCCG GGCCAAGAAC 
TACTACCGCT ACGATCCATC ACACGAACCG GACGACTGGT TCGAGCCGGC CCAGGTCTGG 
GAGGTGCTCT GTGCCGATCT GTCGCTGAGT CCGGTGCATC GGGCCGGCCA GGGCATCATC 
GATCCGGAGA AGGGCATCTC GCTGCGCTTC CCGCGCTTCA TCAAGATCCG CGACGATAAG 
GGAACGACGG ATGCCACCTC GGCCAAACAG GTCGCCGACA TGTACATGAA CCAGGACCAG 
ATCAAGAATC AGCAGCAGAA CAATCAGCGG GACCCCGAAG AGGACTTCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021695617.1
CDS	73..2826
Translation	MFRTTILSVLNVTTQCSVRNLRTVHLARVAYPVLYPLSGQGTNSYPLCCSVLRFYATKRSKKASTVKSEDEDSPMKRSGGTGRKSGAVFDSSPEASPEKKSVDAKTNGGGSVPKRRRIASSSEEEAGGSPKKSSPKKVDEENPIKKEKPSPKVKPKSESKPKKDEKKSPKSSAKKSSPKEKKAVKEEKPTESKDKEVKEVKKEAESPKDKEVKDVKREAESPKKDTKPVDVKDKTANVMSFFTSAGKKDTGSSSGSKVDGADYNPGKKNYHPINDAFWKKGDKVPYLALARTFQIIEETSGRLRMIEILSNYFRSVILLSPSDLLASVYLSLNQLAPAYEGVELGIAEHTLMKAIAQSTGRSLAQIKTDAQNTGDLGLVAEQSKSSQRMMFRPAPHSVEGVFGKLKEIAKMTGAASMAKKVDKIQSMFVACRHSEARFIIRSLAGKLRIGLAEQSLLQALAQACAMTPPNQKDCKEPIVNALSKCSEATAKAKVDEIALILKTVYCQCPNYNQIVPVLLEHGIDHLLEKCPMMPGTPLKPMLAHPTKGVQEVLERFDGIDFTCEWKYDGERAQIHLLEDGTVNIYSRNQENNTSKYPDIIARLGNTRKESVTTAILDCEAVAWDPEKKQILPFQILSTRKRKDANEADIKVQVCVYMFDLLYLNGKPLVERPFLERRELMYKHFIEFEGEWKYATRLDTGDIDELQRFLDEAVKGNCEGLMVKTLQKEATYEIAKRSRNWLKLKKDYLSGVGDSLDLVVIGGYKGRGKRTGTYGGFLLACYDEDNEEYQSICKIGTGFSDEDLQTHTDFFKDKIIPRAKNYYRYDPSHEPDDWFEPAQVWEVLCADLSLSPVHRAGQGIIDPEKGISLRFPRFIKIRDDKGTTDATSAKQVADMYMNQDQIKNQQQNNQRDPEEDFY

Target ORF information:

RefSeq Version	XM_021839925.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti DNA ligase 1 (LOC23687986), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021839925.1

ORF Insert Sequence:

ATGTTCCGAA CTACGATACT TTCCGTTCTG AATGTCACGA CGCAGTGTTC CGTTCGTAAT 
CTGCGAACGG TGCATCTGGC GCGTGTGGCA TATCCCGTGT TGTACCCTCT GAGTGGCCAA 
GGTACTAACA GTTATCCGTT GTGTTGTTCC GTTCTTCGTT TCTACGCCAC CAAACGTAGC 
AAAAAGGCAT CGACCGTTAA ATCCGAAGAT GAGGACAGTC CGATGAAGCG TTCCGGAGGG 
ACTGGACGGA AGTCGGGTGC CGTGTTCGAT TCGTCTCCGG AGGCGTCCCC GGAGAAGAAA 
TCGGTCGATG CGAAGACGAA CGGAGGTGGG AGTGTCCCCA AGCGAAGGCG CATTGCCTCG 
TCCAGTGAGG AGGAAGCAGG AGGATCGCCC AAGAAGTCCA GTCCAAAGAA AGTGGATGAG 
GAGAACCCCA TCAAGAAGGA GAAGCCTAGT CCGAAAGTGA AACCCAAGTC GGAATCGAAA 
CCTAAAAAAG ACGAAAAGAA ATCCCCCAAA TCTTCGGCGA AGAAGAGCTC CCCGAAAGAG 
AAGAAAGCGG TAAAAGAGGA GAAGCCGACT GAGAGCAAGG ACAAGGAAGT GAAGGAAGTT 
AAGAAGGAAG CCGAATCGCC CAAAGACAAA GAAGTGAAGG ATGTCAAGAG GGAAGCTGAA 
TCGCCCAAGA AGGACACTAA ACCGGTGGAC GTGAAGGACA AAACTGCGAA CGTGATGAGT 
TTCTTCACCA GTGCCGGAAA GAAAGATACC GGTTCCAGCA GCGGTTCCAA AGTGGATGGC 
GCCGATTACA ATCCGGGCAA GAAAAACTAC CATCCGATCA ACGACGCCTT CTGGAAGAAG 
GGTGACAAAG TACCGTACTT GGCTTTGGCC AGGACTTTCC AGATAATCGA AGAAACCAGC 
GGGCGGTTGC GGATGATAGA GATTCTGAGT AACTACTTCC GATCGGTGAT TTTGCTCAGT 
CCGAGCGATT TGCTGGCGAG CGTCTATCTC AGTCTGAATC AGTTGGCCCC GGCTTACGAG 
GGCGTTGAGC TGGGTATTGC GGAGCATACG CTGATGAAGG CCATTGCCCA GAGTACGGGA 
CGAAGTCTGG CCCAGATCAA GACCGATGCT CAGAATACGG GAGACTTGGG ACTGGTGGCC 
GAGCAGTCGA AGAGTAGTCA ACGGATGATG TTCCGTCCGG CGCCCCATTC GGTGGAAGGC 
GTCTTTGGGA AGCTTAAGGA AATCGCGAAG ATGACCGGAG CCGCTTCCAT GGCCAAGAAG 
GTCGACAAGA TCCAATCGAT GTTTGTTGCA TGCCGTCACT CGGAAGCTCG TTTCATCATT 
CGCTCGCTGG CCGGGAAGCT GCGGATAGGT CTGGCGGAGC AGTCTTTACT GCAGGCGTTG 
GCTCAAGCAT GTGCCATGAC TCCACCCAAC CAGAAAGATT GCAAAGAACC CATAGTGAAC 
GCGCTGAGCA AGTGCAGTGA AGCAACGGCC AAGGCCAAAG TCGACGAAAT CGCCCTGATC 
CTGAAAACGG TGTACTGTCA GTGCCCGAAC TACAACCAAA TTGTCCCGGT GCTGCTGGAA 
CACGGCATTG ATCACTTGCT AGAGAAATGC CCAATGATGC CGGGAACTCC TCTCAAACCT 
ATGTTGGCCC ATCCCACGAA AGGTGTTCAG GAAGTTCTGG AGCGATTCGA TGGAATCGAC 
TTCACCTGCG AGTGGAAATA CGACGGGGAA CGAGCTCAGA TCCATCTGCT GGAGGATGGC 
ACCGTTAACA TCTATAGCCG TAATCAGGAG AACAACACCA GCAAGTATCC GGACATCATC 
GCGCGGTTAG GCAACACCCG CAAGGAATCT GTAACCACCG CCATCCTGGA TTGCGAAGCC 
GTAGCTTGGG ATCCGGAGAA GAAGCAAATC CTTCCCTTCC AAATCCTCAG CACCCGGAAG 
CGAAAGGACG CCAACGAAGC GGACATCAAG GTCCAGGTGT GCGTCTACAT GTTCGATCTG 
CTGTATCTGA ACGGTAAGCC CCTCGTCGAG CGGCCATTCC TGGAGCGACG AGAACTAATG 
TACAAGCACT TCATCGAGTT CGAAGGCGAG TGGAAGTACG CTACCCGTCT GGACACCGGC 
GACATTGACG AACTGCAGCG CTTCCTGGAC GAAGCCGTCA AGGGCAACTG CGAAGGACTC 
ATGGTCAAAA CCCTCCAGAA GGAAGCCACC TACGAAATCG CCAAACGCTC CCGCAATTGG 
CTCAAGCTCA AAAAGGATTA CCTCTCGGGG GTGGGAGATT CTCTGGATCT GGTCGTAATC 
GGAGGCTACA AGGGCAGGGG CAAGCGAACC GGCACCTACG GAGGTTTCCT CCTTGCTTGC 
TACGACGAAG ACAACGAAGA GTATCAGAGC ATCTGCAAAA TTGGAACCGG ATTCTCCGAC 
GAGGACCTCC AAACGCACAC GGACTTCTTC AAGGACAAAA TCATACCCCG GGCCAAGAAC 
TACTACCGCT ACGATCCATC ACACGAACCG GACGACTGGT TCGAGCCGGC CCAGGTCTGG 
GAGGTGCTCT GTGCCGATCT GTCGCTGAGT CCGGTGCATC GGGCCGGCCA GGGCATCATC 
GATCCGGAGA AGGGCATCTC GCTGCGCTTC CCGCGCTTCA TCAAGATCCG CGACGATAAG 
GGAACGACGG ATGCCACCTC GGCCAAACAG GTCGCCGACA TGTACATGAA CCAGGACCAG 
ATCAAGAATC AGCAGCAGAA CAATCAGCGG GACCCCGAAG AGGACTTCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC23687986 ( XM_011495273.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_011493575.2 Aedes aegypti DNA ligase 1 (LOC23687986), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAe131375
Clone ID Related Accession (Same CDS sequence)	XM_011495273.2
Accession Version	XM_011495273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	DNA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_011495273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAAA AATCAATATT ATCATTTTTC GCCAAAACCA ACGCCAAGAA AGCGGATGAC 
GAATCGTTGG CCGGTCAGCA GGAAAAGAGC GGAAAGGAGA AGCGACGTTC CGACGGCGAA 
GCGGACAATA ACAATGCGGT TAGCAAAAAG GCATCGACCG TTAAATCCGA AGATGAGGAC 
AGTCCGATGA AGCGTTCCGG AGGGACTGGA CGGAAGTCGG GTGCCGTGTT CGATTCGTCT 
CCGGAGGCGT CCCCGGAGAA GAAATCGGTC GATGCGAAGA CGAACGGAGG TGGGAGTGTC 
CCCAAGCGAA GGCGCATTGC CTCGTCCAGT GAGGAGGAAG CAGGAGGATC GCCCAAGAAG 
TCCAGTCCAA AGAAAGTGGA TGAGGAGAAC CCCATCAAGA AGGAGAAGCC TAGTCCGAAA 
GTGAAACCCA AGTCGGAATC GAAACCTAAA AAAGACGAAA AGAAATCCCC CAAATCTTCG 
GCGAAGAAGA GCTCCCCGAA AGAGAAGAAA GCGGTAAAAG AGGAGAAGCC GACTGAGAGC 
AAGGACAAGG AAGTGAAGGA AGTTAAGAAG GAAGCCGAAT CGCCCAAAGA CAAAGAAGTG 
AAGGATGTCA AGAGGGAAGC TGAATCGCCC AAGAAGGACA CTAAACCGGT GGACGTGAAG 
GACAAAACTG CGAACGTGAT GAGTTTCTTC ACCAGTGCCG GAAAGAAAGA TACCGGTTCC 
AGCAGCGGTT CCAAAGTGGA TGGCGCCGAT TACAATCCGG GCAAGAAAAA CTACCATCCG 
ATCAACGACG CCTTCTGGAA GAAGGGTGAC AAAGTACCGT ACTTGGCTTT GGCCAGGACT 
TTCCAGATAA TCGAAGAAAC CAGCGGGCGG TTGCGGATGA TAGAGATTCT GAGTAACTAC 
TTCCGATCGG TGATTTTGCT CAGTCCGAGC GATTTGCTGG CGAGCGTCTA TCTCAGTCTG 
AATCAGTTGG CCCCGGCTTA CGAGGGCGTT GAGCTGGGTA TTGCGGAGCA TACGCTGATG 
AAGGCCATTG CCCAGAGTAC GGGACGAAGT CTGGCCCAGA TCAAGACCGA TGCTCAGAAT 
ACGGGAGACT TGGGACTGGT GGCCGAGCAG TCGAAGAGTA GTCAACGGAT GATGTTCCGT 
CCGGCGCCCC ATTCGGTGGA AGGCGTCTTT GGGAAGCTTA AGGAAATCGC GAAGATGACC 
GGAGCCGCTT CCATGGCCAA GAAGGTCGAC AAGATCCAAT CGATGTTTGT TGCATGCCGT 
CACTCGGAAG CTCGTTTCAT CATTCGCTCG CTGGCCGGGA AGCTGCGGAT AGGTCTGGCG 
GAGCAGTCTT TACTGCAGGC GTTGGCTCAA GCATGTGCCA TGACTCCACC CAACCAGAAA 
GATTGCAAAG AACCCATAGT GAACGCGCTG AGCAAGTGCA GTGAAGCAAC GGCCAAGGCC 
AAAGTCGACG AAATCGCCCT GATCCTGAAA ACGGTGTACT GTCAGTGCCC GAACTACAAC 
CAAATTGTCC CGGTGCTGCT GGAACACGGC ATTGATCACT TGCTAGAGAA ATGCCCAATG 
ATGCCGGGAA CTCCTCTCAA ACCTATGTTG GCCCATCCCA CGAAAGGTGT TCAGGAAGTT 
CTGGAGCGAT TCGATGGAAT CGACTTCACC TGCGAGTGGA AATACGACGG GGAACGAGCT 
CAGATCCATC TGCTGGAGGA TGGCACCGTT AACATCTATA GCCGTAATCA GGAGAACAAC 
ACCAGCAAGT ATCCGGACAT CATCGCGCGG TTAGGCAACA CCCGCAAGGA ATCTGTAACC 
ACCGCCATCC TGGATTGCGA AGCCGTAGCT TGGGATCCGG AGAAGAAGCA AATCCTTCCC 
TTCCAAATCC TCAGCACCCG GAAGCGAAAG GACGCCAACG AAGCGGACAT CAAGGTCCAG 
GTGTGCGTCT ACATGTTCGA TCTGCTGTAT CTGAACGGTA AGCCCCTCGT CGAGCGGCCA 
TTCCTGGAGC GACGAGAACT AATGTACAAG CACTTCATCG AGTTCGAAGG CGAGTGGAAG 
TACGCTACCC GTCTGGACAC CGGCGACATT GACGAACTGC AGCGCTTCCT GGACGAAGCC 
GTCAAGGGCA ACTGCGAAGG ACTCATGGTC AAAACCCTCC AGAAGGAAGC CACCTACGAA 
ATCGCCAAAC GCTCCCGCAA TTGGCTCAAG CTCAAAAAGG ATTACCTCTC GGGGGTGGGA 
GATTCTCTGG ATCTGGTCGT AATCGGAGGC TACAAGGGCA GGGGCAAGCG AACCGGCACC 
TACGGAGGTT TCCTCCTTGC TTGCTACGAC GAAGACAACG AAGAGTATCA GAGCATCTGC 
AAAATTGGAA CCGGATTCTC CGACGAGGAC CTCCAAACGC ACACGGACTT CTTCAAGGAC 
AAAATCATAC CCCGGGCCAA GAACTACTAC CGCTACGATC CATCACACGA ACCGGACGAC 
TGGTTCGAGC CGGCCCAGGT CTGGGAGGTG CTCTGTGCCG ATCTGTCGCT GAGTCCGGTG 
CATCGGGCCG GCCAGGGCAT CATCGATCCG GAGAAGGGCA TCTCGCTGCG CTTCCCGCGC 
TTCATCAAGA TCCGCGACGA TAAGGGAACG ACGGATGCCA CCTCGGCCAA ACAGGTCGCC 
GACATGTACA TGAACCAGGA CCAGATCAAG AATCAGCAGC AGAACAATCA GCGGGACCCC 
GAAGAGGACT TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011493575.2
CDS	122..2839
Translation	MSQKSILSFFAKTNAKKADDESLAGQQEKSGKEKRRSDGEADNNNAVSKKASTVKSEDEDSPMKRSGGTGRKSGAVFDSSPEASPEKKSVDAKTNGGGSVPKRRRIASSSEEEAGGSPKKSSPKKVDEENPIKKEKPSPKVKPKSESKPKKDEKKSPKSSAKKSSPKEKKAVKEEKPTESKDKEVKEVKKEAESPKDKEVKDVKREAESPKKDTKPVDVKDKTANVMSFFTSAGKKDTGSSSGSKVDGADYNPGKKNYHPINDAFWKKGDKVPYLALARTFQIIEETSGRLRMIEILSNYFRSVILLSPSDLLASVYLSLNQLAPAYEGVELGIAEHTLMKAIAQSTGRSLAQIKTDAQNTGDLGLVAEQSKSSQRMMFRPAPHSVEGVFGKLKEIAKMTGAASMAKKVDKIQSMFVACRHSEARFIIRSLAGKLRIGLAEQSLLQALAQACAMTPPNQKDCKEPIVNALSKCSEATAKAKVDEIALILKTVYCQCPNYNQIVPVLLEHGIDHLLEKCPMMPGTPLKPMLAHPTKGVQEVLERFDGIDFTCEWKYDGERAQIHLLEDGTVNIYSRNQENNTSKYPDIIARLGNTRKESVTTAILDCEAVAWDPEKKQILPFQILSTRKRKDANEADIKVQVCVYMFDLLYLNGKPLVERPFLERRELMYKHFIEFEGEWKYATRLDTGDIDELQRFLDEAVKGNCEGLMVKTLQKEATYEIAKRSRNWLKLKKDYLSGVGDSLDLVVIGGYKGRGKRTGTYGGFLLACYDEDNEEYQSICKIGTGFSDEDLQTHTDFFKDKIIPRAKNYYRYDPSHEPDDWFEPAQVWEVLCADLSLSPVHRAGQGIIDPEKGISLRFPRFIKIRDDKGTTDATSAKQVADMYMNQDQIKNQQQNNQRDPEEDFY

Target ORF information:

RefSeq Version	XM_011495273.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti DNA ligase 1 (LOC23687986), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011495273.2

ORF Insert Sequence:

ATGTCCCAAA AATCAATATT ATCATTTTTC GCCAAAACCA ACGCCAAGAA AGCGGATGAC 
GAATCGTTGG CCGGTCAGCA GGAAAAGAGC GGAAAGGAGA AGCGACGTTC CGACGGCGAA 
GCGGACAATA ACAATGCGGT TAGCAAAAAG GCATCGACCG TTAAATCCGA AGATGAGGAC 
AGTCCGATGA AGCGTTCCGG AGGGACTGGA CGGAAGTCGG GTGCCGTGTT CGATTCGTCT 
CCGGAGGCGT CCCCGGAGAA GAAATCGGTC GATGCGAAGA CGAACGGAGG TGGGAGTGTC 
CCCAAGCGAA GGCGCATTGC CTCGTCCAGT GAGGAGGAAG CAGGAGGATC GCCCAAGAAG 
TCCAGTCCAA AGAAAGTGGA TGAGGAGAAC CCCATCAAGA AGGAGAAGCC TAGTCCGAAA 
GTGAAACCCA AGTCGGAATC GAAACCTAAA AAAGACGAAA AGAAATCCCC CAAATCTTCG 
GCGAAGAAGA GCTCCCCGAA AGAGAAGAAA GCGGTAAAAG AGGAGAAGCC GACTGAGAGC 
AAGGACAAGG AAGTGAAGGA AGTTAAGAAG GAAGCCGAAT CGCCCAAAGA CAAAGAAGTG 
AAGGATGTCA AGAGGGAAGC TGAATCGCCC AAGAAGGACA CTAAACCGGT GGACGTGAAG 
GACAAAACTG CGAACGTGAT GAGTTTCTTC ACCAGTGCCG GAAAGAAAGA TACCGGTTCC 
AGCAGCGGTT CCAAAGTGGA TGGCGCCGAT TACAATCCGG GCAAGAAAAA CTACCATCCG 
ATCAACGACG CCTTCTGGAA GAAGGGTGAC AAAGTACCGT ACTTGGCTTT GGCCAGGACT 
TTCCAGATAA TCGAAGAAAC CAGCGGGCGG TTGCGGATGA TAGAGATTCT GAGTAACTAC 
TTCCGATCGG TGATTTTGCT CAGTCCGAGC GATTTGCTGG CGAGCGTCTA TCTCAGTCTG 
AATCAGTTGG CCCCGGCTTA CGAGGGCGTT GAGCTGGGTA TTGCGGAGCA TACGCTGATG 
AAGGCCATTG CCCAGAGTAC GGGACGAAGT CTGGCCCAGA TCAAGACCGA TGCTCAGAAT 
ACGGGAGACT TGGGACTGGT GGCCGAGCAG TCGAAGAGTA GTCAACGGAT GATGTTCCGT 
CCGGCGCCCC ATTCGGTGGA AGGCGTCTTT GGGAAGCTTA AGGAAATCGC GAAGATGACC 
GGAGCCGCTT CCATGGCCAA GAAGGTCGAC AAGATCCAAT CGATGTTTGT TGCATGCCGT 
CACTCGGAAG CTCGTTTCAT CATTCGCTCG CTGGCCGGGA AGCTGCGGAT AGGTCTGGCG 
GAGCAGTCTT TACTGCAGGC GTTGGCTCAA GCATGTGCCA TGACTCCACC CAACCAGAAA 
GATTGCAAAG AACCCATAGT GAACGCGCTG AGCAAGTGCA GTGAAGCAAC GGCCAAGGCC 
AAAGTCGACG AAATCGCCCT GATCCTGAAA ACGGTGTACT GTCAGTGCCC GAACTACAAC 
CAAATTGTCC CGGTGCTGCT GGAACACGGC ATTGATCACT TGCTAGAGAA ATGCCCAATG 
ATGCCGGGAA CTCCTCTCAA ACCTATGTTG GCCCATCCCA CGAAAGGTGT TCAGGAAGTT 
CTGGAGCGAT TCGATGGAAT CGACTTCACC TGCGAGTGGA AATACGACGG GGAACGAGCT 
CAGATCCATC TGCTGGAGGA TGGCACCGTT AACATCTATA GCCGTAATCA GGAGAACAAC 
ACCAGCAAGT ATCCGGACAT CATCGCGCGG TTAGGCAACA CCCGCAAGGA ATCTGTAACC 
ACCGCCATCC TGGATTGCGA AGCCGTAGCT TGGGATCCGG AGAAGAAGCA AATCCTTCCC 
TTCCAAATCC TCAGCACCCG GAAGCGAAAG GACGCCAACG AAGCGGACAT CAAGGTCCAG 
GTGTGCGTCT ACATGTTCGA TCTGCTGTAT CTGAACGGTA AGCCCCTCGT CGAGCGGCCA 
TTCCTGGAGC GACGAGAACT AATGTACAAG CACTTCATCG AGTTCGAAGG CGAGTGGAAG 
TACGCTACCC GTCTGGACAC CGGCGACATT GACGAACTGC AGCGCTTCCT GGACGAAGCC 
GTCAAGGGCA ACTGCGAAGG ACTCATGGTC AAAACCCTCC AGAAGGAAGC CACCTACGAA 
ATCGCCAAAC GCTCCCGCAA TTGGCTCAAG CTCAAAAAGG ATTACCTCTC GGGGGTGGGA 
GATTCTCTGG ATCTGGTCGT AATCGGAGGC TACAAGGGCA GGGGCAAGCG AACCGGCACC 
TACGGAGGTT TCCTCCTTGC TTGCTACGAC GAAGACAACG AAGAGTATCA GAGCATCTGC 
AAAATTGGAA CCGGATTCTC CGACGAGGAC CTCCAAACGC ACACGGACTT CTTCAAGGAC 
AAAATCATAC CCCGGGCCAA GAACTACTAC CGCTACGATC CATCACACGA ACCGGACGAC 
TGGTTCGAGC CGGCCCAGGT CTGGGAGGTG CTCTGTGCCG ATCTGTCGCT GAGTCCGGTG 
CATCGGGCCG GCCAGGGCAT CATCGATCCG GAGAAGGGCA TCTCGCTGCG CTTCCCGCGC 
TTCATCAAGA TCCGCGACGA TAAGGGAACG ACGGATGCCA CCTCGGCCAA ACAGGTCGCC 
GACATGTACA TGAACCAGGA CCAGATCAAG AATCAGCAGC AGAACAATCA GCGGGACCCC 
GAAGAGGACT TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC23687986 ( XM_011495273.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_011493575.1 Aedes aegypti AAEL017566-RA mRNA.

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CloneID	OAe15211
Clone ID Related Accession (Same CDS sequence)	XM_011495273.1
Accession Version	XM_011495273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL017566-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811279).

ATGTCCCAAA AATCAATATT ATCATTTTTC GCCAAAACCA ACGTCAAGAA AGCGGATGAC 
GAATCGTTGG CCGGTCAGCA GGAAAAGAGC GGAAAGGAGA AGCGACGTTC CGACGGCGAA 
GCGGACAATA ACAATGCGGT TAGCAAAAAG GCATCGACCG TTAAATCTGA AGATGAGGAC 
AGTCCGGTCA AGCGTTCCGG TGGGGCTGGA CGGAAGTCGG CTGCCGTGTT CGATTCGTCT 
CCGGAGGCGT CCCCGGAGAA GAAATCGGTC GATGCGAAGA CGAACGGAGG TGGGAGTGTC 
CCCAAGCGAA GGCGCATTGC CTCGTCTAGT GAGGAGGAAG ACGGAGGATC GCCCAAGAAG 
TCCAGTCCAA AGAAAGTGGA AGAGGAGAAA CCCATCAAGA AGGAGAAGCC TAGTCCGAAA 
GTGAAACCCA AGTCGGAATC GAAACCTAAA CAAGAGGAAA AGAAATCCCC CAAATCTTCG 
GCGAAGAAGA GCTCTCCGAA AGAGAAGAAA GCGGTAAAAG ATGAGAAGCC GACTGAGAGC 
AAGGACAAGG AAGTGAAGGA AGTTAAGAAG GAAGCCGAAT CACCCAAAGA CAAAGAAGTG 
AAGGAAGTTA AGAAGGAAGC CGAATCGCCC AAGAAGGACA CTAAACCGGT GGACGTGAAG 
GACAAAACTG CGAACGTGAT GAGTTTCTTC ACCAGTGCCG GAAAGAAAGA TACCGGTTCC 
GGGAGCGGCT CCAAAGTGGA TGGCGCTGAT TACAATCCGG GCAAGAAAAA CTACCATCCG 
ATCAACGACG CCTTCTGGAA GAAGGGCGAC AAAGTGCCGT ACCTGGCATT GGCCAGGACT 
TTCCAGATAA TCGAAGAAAC CAGCGGGCGG TTGCGGATGA TAGAGATTCT GAGCAACTAC 
TTCCGATCGG TGATTTTGCT CAGTCCGAGC GATTTGCTGG CAAGCGTCTA TCTCAGTCTG 
AATCAGTTGG CCCCGGCTTA CGAGGGCGTT GAACTGGGAA TTGCCGAGCA TACGCTGATG 
AAGGCCATTG CCCAGAGTAC AGGACGAAGT CTGGCCCAGA TCAAGACCGA TGCCCAGAAT 
ACGGGGGACT TGGGACTGGT GGCCGAGCAG TCGAAGAGTA GTCAACGGAT GATGTTCCGT 
CCGGCGCCCC ATTCGGTGGA GGGTGTCTTT GGAAAACTCA AGGAAATCGC GAAGATGACC 
GGAGCCGCTT CCATGGCCAA GAAGGTCGAC AAGATCCAAT CGATGTTTGT TGCATGCCGT 
CACTCGGAAG CTCGTTTCAT CATTCGCTCG CTGGCCGGGA AGCTGCGGAT AGGTCTGGCG 
GAGCAGTCTT TGCTGCAGGC GTTGGCTCAA GCATGTGCCA TGACTCCACC CAACCAGAAA 
GACTGCAAAG AACCCATCGT GAACGCGCTG AGCAAGTGCA GTGAAGCGAC GGCCAAGGCC 
AAAGTCGACG AAATCGCCCT GATCCTGAAA ACGGTGTACT GTCAGTGCCC GAACTACAAC 
CAGATTGTCC CAGTGCTGCT GGAACACGGC ATTGATCACT TGCTAGAGAA ATGCCCAATG 
ATGCCGGGAA CTCCTCTCAA ACCTATGTTG GCCCATCCCA CGAAAGGTGT TCAGGAAGTT 
CTGGAGCGAT TCGATGGCAT CGACTTCACC TGCGAATGGA AATACGACGG GGAACGAGCT 
CAGATCCATC TGCTGGAGGA TGGCACCGTC AACATCTATA GCCGTAATCA GGAGAACAAC 
ACCAGCAAGT ATCCGGACAT CATCGCGCGG TTAGGCAACA CCCGGAAGGA ATCGGTGACC 
AGCGCCATCC TGGACTGCGA AGCCGTAGCT TGGGATCCGG AGAAGAAGCA AATCCTTCCT 
TTCCAAATCC TCAGCACCCG GAAGCGGAAG GACGCCAACG AAGCGGACAT CAAGGTCCAG 
GTGTGCGTCT ACATGTTCGA TCTGTTGTAT CTGAACGGTA AGCCCCTCGT GGAGCGGCCA 
TTCCTGGAGC GACGAGAACT AATGTACAAG CACTTCATCG AGTTCGAAGG CGAGTGGAAG 
TACGCTACCC GTCTGGACAC CGGCGACATT GACGAACTGC AGCGCTTCCT GGACGAAGCC 
GTCAAGGGCA ACTGCGAAGG ACTCATGGTC AAAACCCTCC AGAAGGAAGC CACCTACGAA 
ATCGCCAAAC GCTCCCGCAA TTGGCTCAAG CTCAAAAAGG ATTACCTCTC GGGGGTGGGA 
GATTCTCTGG ATCTGGTCGT AATCGGAGGC TACAAGGGCA GGGGCAAGCG AACCGGCACC 
TACGGAGGTT TCCTCCTTGC CTGCTACGAC GAAGACAACG AAGAGTATCA GAGCATCTGC 
AAAATTGGAA CCGGATTCTC CGACGAGGAC CTCCAAACGC ACACGGACTT CTTCAAGGAC 
AAAATCATCC CCCGGGCCAA GAACTACTAC CGCTACGATT CATCACACGA GCCGGACGAC 
TGGTTCGAGC CGGCCCAGGT CTGGGAGGTG CTCTGTGCCG ATCTGTCGCT GAGTCCAGTG 
CATCGGGCCG GCCAGGGCAT CATCGATCCG GAGAAGGGCA TCTCGCTGCG CTTTCCGCGC 
TTCATCAAGA TCCGCGACGA TAAGGGAACG ACGGATGCCA CCTCGGCCAA ACAGGTCGCC 
GACATGTACA TGAACCAGGA CCAGATCAAG AATCAGCAGC AGAGCAACCA GAGAGACCCC 
GAAGAGGACT TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011493575.1
CDS	81..2798
Translation	MSQKSILSFFAKTNVKKADDESLAGQQEKSGKEKRRSDGEADNNNAVSKKASTVKSEDEDSPVKRSGGAGRKSAAVFDSSPEASPEKKSVDAKTNGGGSVPKRRRIASSSEEEDGGSPKKSSPKKVEEEKPIKKEKPSPKVKPKSESKPKQEEKKSPKSSAKKSSPKEKKAVKDEKPTESKDKEVKEVKKEAESPKDKEVKEVKKEAESPKKDTKPVDVKDKTANVMSFFTSAGKKDTGSGSGSKVDGADYNPGKKNYHPINDAFWKKGDKVPYLALARTFQIIEETSGRLRMIEILSNYFRSVILLSPSDLLASVYLSLNQLAPAYEGVELGIAEHTLMKAIAQSTGRSLAQIKTDAQNTGDLGLVAEQSKSSQRMMFRPAPHSVEGVFGKLKEIAKMTGAASMAKKVDKIQSMFVACRHSEARFIIRSLAGKLRIGLAEQSLLQALAQACAMTPPNQKDCKEPIVNALSKCSEATAKAKVDEIALILKTVYCQCPNYNQIVPVLLEHGIDHLLEKCPMMPGTPLKPMLAHPTKGVQEVLERFDGIDFTCEWKYDGERAQIHLLEDGTVNIYSRNQENNTSKYPDIIARLGNTRKESVTSAILDCEAVAWDPEKKQILPFQILSTRKRKDANEADIKVQVCVYMFDLLYLNGKPLVERPFLERRELMYKHFIEFEGEWKYATRLDTGDIDELQRFLDEAVKGNCEGLMVKTLQKEATYEIAKRSRNWLKLKKDYLSGVGDSLDLVVIGGYKGRGKRTGTYGGFLLACYDEDNEEYQSICKIGTGFSDEDLQTHTDFFKDKIIPRAKNYYRYDSSHEPDDWFEPAQVWEVLCADLSLSPVHRAGQGIIDPEKGISLRFPRFIKIRDDKGTTDATSAKQVADMYMNQDQIKNQQQSNQRDPEEDFY

Target ORF information:

RefSeq Version	XM_011495273.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL017566-RA mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011495273.1

ORF Insert Sequence:

ATGTCCCAAA AATCAATATT ATCATTTTTC GCCAAAACCA ACGTCAAGAA AGCGGATGAC 
GAATCGTTGG CCGGTCAGCA GGAAAAGAGC GGAAAGGAGA AGCGACGTTC CGACGGCGAA 
GCGGACAATA ACAATGCGGT TAGCAAAAAG GCATCGACCG TTAAATCTGA AGATGAGGAC 
AGTCCGGTCA AGCGTTCCGG TGGGGCTGGA CGGAAGTCGG CTGCCGTGTT CGATTCGTCT 
CCGGAGGCGT CCCCGGAGAA GAAATCGGTC GATGCGAAGA CGAACGGAGG TGGGAGTGTC 
CCCAAGCGAA GGCGCATTGC CTCGTCTAGT GAGGAGGAAG ACGGAGGATC GCCCAAGAAG 
TCCAGTCCAA AGAAAGTGGA AGAGGAGAAA CCCATCAAGA AGGAGAAGCC TAGTCCGAAA 
GTGAAACCCA AGTCGGAATC GAAACCTAAA CAAGAGGAAA AGAAATCCCC CAAATCTTCG 
GCGAAGAAGA GCTCTCCGAA AGAGAAGAAA GCGGTAAAAG ATGAGAAGCC GACTGAGAGC 
AAGGACAAGG AAGTGAAGGA AGTTAAGAAG GAAGCCGAAT CACCCAAAGA CAAAGAAGTG 
AAGGAAGTTA AGAAGGAAGC CGAATCGCCC AAGAAGGACA CTAAACCGGT GGACGTGAAG 
GACAAAACTG CGAACGTGAT GAGTTTCTTC ACCAGTGCCG GAAAGAAAGA TACCGGTTCC 
GGGAGCGGCT CCAAAGTGGA TGGCGCTGAT TACAATCCGG GCAAGAAAAA CTACCATCCG 
ATCAACGACG CCTTCTGGAA GAAGGGCGAC AAAGTGCCGT ACCTGGCATT GGCCAGGACT 
TTCCAGATAA TCGAAGAAAC CAGCGGGCGG TTGCGGATGA TAGAGATTCT GAGCAACTAC 
TTCCGATCGG TGATTTTGCT CAGTCCGAGC GATTTGCTGG CAAGCGTCTA TCTCAGTCTG 
AATCAGTTGG CCCCGGCTTA CGAGGGCGTT GAACTGGGAA TTGCCGAGCA TACGCTGATG 
AAGGCCATTG CCCAGAGTAC AGGACGAAGT CTGGCCCAGA TCAAGACCGA TGCCCAGAAT 
ACGGGGGACT TGGGACTGGT GGCCGAGCAG TCGAAGAGTA GTCAACGGAT GATGTTCCGT 
CCGGCGCCCC ATTCGGTGGA GGGTGTCTTT GGAAAACTCA AGGAAATCGC GAAGATGACC 
GGAGCCGCTT CCATGGCCAA GAAGGTCGAC AAGATCCAAT CGATGTTTGT TGCATGCCGT 
CACTCGGAAG CTCGTTTCAT CATTCGCTCG CTGGCCGGGA AGCTGCGGAT AGGTCTGGCG 
GAGCAGTCTT TGCTGCAGGC GTTGGCTCAA GCATGTGCCA TGACTCCACC CAACCAGAAA 
GACTGCAAAG AACCCATCGT GAACGCGCTG AGCAAGTGCA GTGAAGCGAC GGCCAAGGCC 
AAAGTCGACG AAATCGCCCT GATCCTGAAA ACGGTGTACT GTCAGTGCCC GAACTACAAC 
CAGATTGTCC CAGTGCTGCT GGAACACGGC ATTGATCACT TGCTAGAGAA ATGCCCAATG 
ATGCCGGGAA CTCCTCTCAA ACCTATGTTG GCCCATCCCA CGAAAGGTGT TCAGGAAGTT 
CTGGAGCGAT TCGATGGCAT CGACTTCACC TGCGAATGGA AATACGACGG GGAACGAGCT 
CAGATCCATC TGCTGGAGGA TGGCACCGTC AACATCTATA GCCGTAATCA GGAGAACAAC 
ACCAGCAAGT ATCCGGACAT CATCGCGCGG TTAGGCAACA CCCGGAAGGA ATCGGTGACC 
AGCGCCATCC TGGACTGCGA AGCCGTAGCT TGGGATCCGG AGAAGAAGCA AATCCTTCCT 
TTCCAAATCC TCAGCACCCG GAAGCGGAAG GACGCCAACG AAGCGGACAT CAAGGTCCAG 
GTGTGCGTCT ACATGTTCGA TCTGTTGTAT CTGAACGGTA AGCCCCTCGT GGAGCGGCCA 
TTCCTGGAGC GACGAGAACT AATGTACAAG CACTTCATCG AGTTCGAAGG CGAGTGGAAG 
TACGCTACCC GTCTGGACAC CGGCGACATT GACGAACTGC AGCGCTTCCT GGACGAAGCC 
GTCAAGGGCA ACTGCGAAGG ACTCATGGTC AAAACCCTCC AGAAGGAAGC CACCTACGAA 
ATCGCCAAAC GCTCCCGCAA TTGGCTCAAG CTCAAAAAGG ATTACCTCTC GGGGGTGGGA 
GATTCTCTGG ATCTGGTCGT AATCGGAGGC TACAAGGGCA GGGGCAAGCG AACCGGCACC 
TACGGAGGTT TCCTCCTTGC CTGCTACGAC GAAGACAACG AAGAGTATCA GAGCATCTGC 
AAAATTGGAA CCGGATTCTC CGACGAGGAC CTCCAAACGC ACACGGACTT CTTCAAGGAC 
AAAATCATCC CCCGGGCCAA GAACTACTAC CGCTACGATT CATCACACGA GCCGGACGAC 
TGGTTCGAGC CGGCCCAGGT CTGGGAGGTG CTCTGTGCCG ATCTGTCGCT GAGTCCAGTG 
CATCGGGCCG GCCAGGGCAT CATCGATCCG GAGAAGGGCA TCTCGCTGCG CTTTCCGCGC 
TTCATCAAGA TCCGCGACGA TAAGGGAACG ACGGATGCCA CCTCGGCCAA ACAGGTCGCC 
GACATGTACA TGAACCAGGA CCAGATCAAG AATCAGCAGC AGAGCAACCA GAGAGACCCC 
GAAGAGGACT TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC23687986 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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