OT_ostta08g02510 cDNA ORF clone, Ostreococcus tauri

Gene
Clones
gRNAs

Summary

Gene Symbol	OT_ostta08g02510
Entrez Gene ID	9831633
Full Name	Carbamoyl-phosphate synthase large subunit,CPSase domain
Synonyms	Ot08g02450
Gene Type	protein-coding
Organism	Ostreococcus tauri

mRNA and Protein(s)

mRNA	Protein	Name
XM_003080767.1	XP_003080815.1	putative carbamoyl phosphate synthetase (ISS)
XM_022983593.1	XP_022840996.1	Carbamoyl-phosphate synthase large subunit,CPSase domain

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.
Blanc-Mathieu R, Verhelst B, Derelle E, Rombauts S, Bouget FY, Carré I, Château A, Eyre-Walker A, Grimsley N, Moreau H, Piégu B, Rivals E, Schackwitz W, Van de Peer Y, Piganeau G
BMC genomics151103(2014 Dec)

Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features.
Derelle E, Ferraz C, Rombauts S, Rouzé P, Worden AZ, Robbens S, Partensky F, Degroeve S, Echeynié S, Cooke R, Saeys Y, Wuyts J, Jabbari K, Bowler C, Panaud O, Piégu B, Ball SG, Ral JP, Bouget FY, Piganeau G, De Baets B, Picard A, Delseny M, Demaille J, Van de Peer Y, Moreau H
Proceedings of the National Academy of Sciences of the United States of America103(31)11647-52(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following OT_ostta08g02510 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OT_ostta08g02510 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOd00815	XM_003080767.1 Latest version!	Ostreococcus tauri putative carbamoyl phosphate synthetase (ISS) (Ot08g02450) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOd76309	XM_022983593.1 Latest version!	Ostreococcus tauri Carbamoyl-phosphate synthase large subunit,CPSase domain (OT_ostta08g02510), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OT_ostta08g02510 ( XM_003080767.1 ) cDNA ORF clone, Ostreococcus tauri -> XP_003080815.1 Ostreococcus tauri putative carbamoyl phosphate synthetase (ISS) (Ot08g02450) mRNA, complete cds.

Price & Availability↑

CloneID	OOd00815
Clone ID Related Accession (Same CDS sequence)	XM_003080767.1
Accession Version	XM_003080767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1373472000000
Organism	Ostreococcus tauri
Product	putative carbamoyl phosphate synthetase (ISS)
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_014433). COMPLETENESS: incomplete on both ends.

ATGGGCGTGC ACACTGGTGA TTCGATCACC ATCGCACCGG CTCAAACGCT CACCGACAAG 
GAATACCAGC GTCTTCGTGA TGCCTCGGTT GCCATCATCC GTGAGATTGG CGTCGAGTGT 
GGTGGTTCCA ACGTTCAGAT GGCTGTCAAC CCGGTCGACG GCCAGGTCAT GATCATTGAA 
ATGAACCCGC GTGTGTCTCG CTCGTCTGCT CTTGCGTCCA AGGCGACCGG TTTCCCGATC 
GCTAAGATGG CCGCGAAGCT CGCTGTCGGT TGCACTCTCG ACGGTATCCC TAACGACATC 
ACACTCAAGA CCCCGGCGTC TTTCGAGCCT TCCATCGATT ATGTCGTCAC GAAGATTCCT 
CGATTCGCTT TCGAAAAGTT CCCGGGCGCG AAGCCCGTGC TTACGACACA GATGAAGTCT 
GTCGGTGAGG CCATGGCCAT GGGTCGCACT TGGCAAGAGT CTTTCCAAAA GGCGTTCCGT 
TCTCTCGAAA CCGGCTTCGC CGGTTGGGGT CTCGGTAAGA AGGATGGGTT CATGCAAGGT 
GACGTTGCTG CGATCAACGA GGGCCTACTC GTGCCGAACC CCGAGCGCAT TGTTGTCATT 
CATGAAGCGT TCCTCGCGGG TTTCACGGAG GATCAGATCA TCAAGGCAAC GACGATGGAC 
CCATGGTTCA TCCGACAGCT CGGTGAACTC TACCAGACCG AGTGCTGGCT CAAATCGCTC 
AAGTCACTCG ACGATCTTTC CGAGAACGAT TGGTTCGAAG TTAAGAGGCG TGGCTACAGC 
GATTCACAAA TTTCCATGGC TTTCCCGGGC ACGACTGAGT TGCAAGTTCG CAAGGAGCGC 
ACGGCGAAGG GTTGCAAGCC CTCCATGAAG CGCGTGGATA CGTGCGCGGC CGAATTCGTT 
GCGGACACTC CGTATATGTA TTCCTCGTAC GACGGCTTCG ACGAATCTGA ACCGACGGAC 
AACAAGAAAA TCCTCATTCT GGGTGGTGGT CCGAACCGCA TCGGTCAAGG TATTGAGTTC 
GACTACTGCT GCTGCCATGC CGCGTTCGCC CTCGCGGACG CTGGTTACGA AACCATCATG 
CTCAACAGTA ACCCGGAGAC TGTGTCTACC GACTACGATA CCTCTGATCG CCTGTACTTC 
GAACCGCTGA CGGTTGAAGA TGTCTTGAAT GTGTGCGAAG TTGAGCGTCC GGAGGGCATC 
ATCGTTCAGT TCGGTGGCCA AACTCCTTTG AGCCTCGCGA CAAGGCTTGA AGCTGCCCTC 
AAGGAGAACC CGATTCCGGC GGCGTCCGGC AACGGTTTTA CACAGATTTT GGGTACCCCT 
CCGGACTCAA TCGATGCTGC AGAAGATCGC GAGCGCTGGA TCGATATTTT GGAAGAACTC 
AACATTCTTC AACCGCCGGG AGGTGTTGCG AGATCTGAGG AAGAAGCGTT GAAGATTGCT 
GAAAAACTTA CCTACCCTGT CATGGTTCGT CCGTCTTACG TCCTCGGTGG GCGCGCCATG 
GAGATCGTTG GCTCTAACGC CGACTTGAAG CGTTACATCA AGACTGCCGT CGAAGTCGAC 
GAAGATCGCC CGGTGCTTGT CGATAAGTAC CTCCAAAACG CGACCGAGAT CGATTGCGAT 
GCATTGTGCG ATAAGGATGG CAACGTTGTC ATCGGTGGTA TCATGGAACA CATTGAGCAA 
GCGGGAGTGC ACTCTGGTGA CTCTGCGTGC TCCTTACCAA CGCAAACTAT CCCTGAATCT 
GCCCTTGCTA CCATTCGTGA ATGGACCCCG AAGCTCGCGA AACGACTCGG TGTCATCGGT 
CTCATCAACA TTCAGTACGC TGTGACCCCG GAGGGTACGC CGTACATCAT TGAGGCGAAC 
CCGCGTGCTT CTCGTACTGT GCCGTTCGTG GCGAAGGCGA TTGGTCATCC GTTGGCTAAG 
TACGCGTCGT TGGTGATGGC TGGAAAGACG CTCAAGGAAA TCGGCTTCAC GGAAGAAGTC 
AAATTGAACC ACGTCGCCGT GAAGGAAGCG GTCCTTCCGT TCGACAAGTT CCCGGGTGCG 
GACACTTTGC TTGGTCCGGA GATGAGAAGT ACTGGTGAGG TGATGGGCAT TGATCGTGAC 
TTCAGCCGTG CTTATTGCAA GGCCCAGCTC GCTGCGGGAC AGCGACTGCC CACCTCCGGC 
AAGGTCTTCA TTTCCGTGCG CGATTCTGAC AAGGACGCCA TAGTTGACAT AGCTCGTGAC 
TTCGTCAGCA TGAACTACAC GGTCGTGTCG ACCGGTGGTA CGGCGACTCA CTTAGAAAAG 
GCTGGTGTTG CTGTCACTAA GGTGCTCAAG TTGCACGAAG GCCGACCGAA CGTCTCCGAC 
ATGATTCGTG ATGGTGAAAT TGGTATGATG GTGGTTACCT CGTCTGGTGA CGCCCAAGAT 
TTGGTGGATG GCCGCAAGAT CCGTCGCGAG GCGGTTGGCC TGAAGGTGCC GCTTGTCACG 
ACCATTGCCG GCGCGAAGGC CACGGCCGGT GCGGTTCGCG TCTTGCAGAA GGGTGACCTC 
AAAATGGACG CGCTGCAAGA CTTTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003080815.1
CDS	1..2550
Translation	MGVHTGDSITIAPAQTLTDKEYQRLRDASVAIIREIGVECGGSNVQMAVNPVDGQVMIIEMNPRVSRSSALASKATGFPIAKMAAKLAVGCTLDGIPNDITLKTPASFEPSIDYVVTKIPRFAFEKFPGAKPVLTTQMKSVGEAMAMGRTWQESFQKAFRSLETGFAGWGLGKKDGFMQGDVAAINEGLLVPNPERIVVIHEAFLAGFTEDQIIKATTMDPWFIRQLGELYQTECWLKSLKSLDDLSENDWFEVKRRGYSDSQISMAFPGTTELQVRKERTAKGCKPSMKRVDTCAAEFVADTPYMYSSYDGFDESEPTDNKKILILGGGPNRIGQGIEFDYCCCHAAFALADAGYETIMLNSNPETVSTDYDTSDRLYFEPLTVEDVLNVCEVERPEGIIVQFGGQTPLSLATRLEAALKENPIPAASGNGFTQILGTPPDSIDAAEDRERWIDILEELNILQPPGGVARSEEEALKIAEKLTYPVMVRPSYVLGGRAMEIVGSNADLKRYIKTAVEVDEDRPVLVDKYLQNATEIDCDALCDKDGNVVIGGIMEHIEQAGVHSGDSACSLPTQTIPESALATIREWTPKLAKRLGVIGLINIQYAVTPEGTPYIIEANPRASRTVPFVAKAIGHPLAKYASLVMAGKTLKEIGFTEEVKLNHVAVKEAVLPFDKFPGADTLLGPEMRSTGEVMGIDRDFSRAYCKAQLAAGQRLPTSGKVFISVRDSDKDAIVDIARDFVSMNYTVVSTGGTATHLEKAGVAVTKVLKLHEGRPNVSDMIRDGEIGMMVVTSSGDAQDLVDGRKIRREAVGLKVPLVTTIAGAKATAGAVRVLQKGDLKMDALQDFF

Target ORF information:

RefSeq Version	XM_003080767.1
Organism	Ostreococcus tauri
Definition	Ostreococcus tauri putative carbamoyl phosphate synthetase (ISS) (Ot08g02450) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003080767.1

ORF Insert Sequence:

ATGGGCGTGC ACACTGGTGA TTCGATCACC ATCGCACCGG CTCAAACGCT CACCGACAAG 
GAATACCAGC GTCTTCGTGA TGCCTCGGTT GCCATCATCC GTGAGATTGG CGTCGAGTGT 
GGTGGTTCCA ACGTTCAGAT GGCTGTCAAC CCGGTCGACG GCCAGGTCAT GATCATTGAA 
ATGAACCCGC GTGTGTCTCG CTCGTCTGCT CTTGCGTCCA AGGCGACCGG TTTCCCGATC 
GCTAAGATGG CCGCGAAGCT CGCTGTCGGT TGCACTCTCG ACGGTATCCC TAACGACATC 
ACACTCAAGA CCCCGGCGTC TTTCGAGCCT TCCATCGATT ATGTCGTCAC GAAGATTCCT 
CGATTCGCTT TCGAAAAGTT CCCGGGCGCG AAGCCCGTGC TTACGACACA GATGAAGTCT 
GTCGGTGAGG CCATGGCCAT GGGTCGCACT TGGCAAGAGT CTTTCCAAAA GGCGTTCCGT 
TCTCTCGAAA CCGGCTTCGC CGGTTGGGGT CTCGGTAAGA AGGATGGGTT CATGCAAGGT 
GACGTTGCTG CGATCAACGA GGGCCTACTC GTGCCGAACC CCGAGCGCAT TGTTGTCATT 
CATGAAGCGT TCCTCGCGGG TTTCACGGAG GATCAGATCA TCAAGGCAAC GACGATGGAC 
CCATGGTTCA TCCGACAGCT CGGTGAACTC TACCAGACCG AGTGCTGGCT CAAATCGCTC 
AAGTCACTCG ACGATCTTTC CGAGAACGAT TGGTTCGAAG TTAAGAGGCG TGGCTACAGC 
GATTCACAAA TTTCCATGGC TTTCCCGGGC ACGACTGAGT TGCAAGTTCG CAAGGAGCGC 
ACGGCGAAGG GTTGCAAGCC CTCCATGAAG CGCGTGGATA CGTGCGCGGC CGAATTCGTT 
GCGGACACTC CGTATATGTA TTCCTCGTAC GACGGCTTCG ACGAATCTGA ACCGACGGAC 
AACAAGAAAA TCCTCATTCT GGGTGGTGGT CCGAACCGCA TCGGTCAAGG TATTGAGTTC 
GACTACTGCT GCTGCCATGC CGCGTTCGCC CTCGCGGACG CTGGTTACGA AACCATCATG 
CTCAACAGTA ACCCGGAGAC TGTGTCTACC GACTACGATA CCTCTGATCG CCTGTACTTC 
GAACCGCTGA CGGTTGAAGA TGTCTTGAAT GTGTGCGAAG TTGAGCGTCC GGAGGGCATC 
ATCGTTCAGT TCGGTGGCCA AACTCCTTTG AGCCTCGCGA CAAGGCTTGA AGCTGCCCTC 
AAGGAGAACC CGATTCCGGC GGCGTCCGGC AACGGTTTTA CACAGATTTT GGGTACCCCT 
CCGGACTCAA TCGATGCTGC AGAAGATCGC GAGCGCTGGA TCGATATTTT GGAAGAACTC 
AACATTCTTC AACCGCCGGG AGGTGTTGCG AGATCTGAGG AAGAAGCGTT GAAGATTGCT 
GAAAAACTTA CCTACCCTGT CATGGTTCGT CCGTCTTACG TCCTCGGTGG GCGCGCCATG 
GAGATCGTTG GCTCTAACGC CGACTTGAAG CGTTACATCA AGACTGCCGT CGAAGTCGAC 
GAAGATCGCC CGGTGCTTGT CGATAAGTAC CTCCAAAACG CGACCGAGAT CGATTGCGAT 
GCATTGTGCG ATAAGGATGG CAACGTTGTC ATCGGTGGTA TCATGGAACA CATTGAGCAA 
GCGGGAGTGC ACTCTGGTGA CTCTGCGTGC TCCTTACCAA CGCAAACTAT CCCTGAATCT 
GCCCTTGCTA CCATTCGTGA ATGGACCCCG AAGCTCGCGA AACGACTCGG TGTCATCGGT 
CTCATCAACA TTCAGTACGC TGTGACCCCG GAGGGTACGC CGTACATCAT TGAGGCGAAC 
CCGCGTGCTT CTCGTACTGT GCCGTTCGTG GCGAAGGCGA TTGGTCATCC GTTGGCTAAG 
TACGCGTCGT TGGTGATGGC TGGAAAGACG CTCAAGGAAA TCGGCTTCAC GGAAGAAGTC 
AAATTGAACC ACGTCGCCGT GAAGGAAGCG GTCCTTCCGT TCGACAAGTT CCCGGGTGCG 
GACACTTTGC TTGGTCCGGA GATGAGAAGT ACTGGTGAGG TGATGGGCAT TGATCGTGAC 
TTCAGCCGTG CTTATTGCAA GGCCCAGCTC GCTGCGGGAC AGCGACTGCC CACCTCCGGC 
AAGGTCTTCA TTTCCGTGCG CGATTCTGAC AAGGACGCCA TAGTTGACAT AGCTCGTGAC 
TTCGTCAGCA TGAACTACAC GGTCGTGTCG ACCGGTGGTA CGGCGACTCA CTTAGAAAAG 
GCTGGTGTTG CTGTCACTAA GGTGCTCAAG TTGCACGAAG GCCGACCGAA CGTCTCCGAC 
ATGATTCGTG ATGGTGAAAT TGGTATGATG GTGGTTACCT CGTCTGGTGA CGCCCAAGAT 
TTGGTGGATG GCCGCAAGAT CCGTCGCGAG GCGGTTGGCC TGAAGGTGCC GCTTGTCACG 
ACCATTGCCG GCGCGAAGGC CACGGCCGGT GCGGTTCGCG TCTTGCAGAA GGGTGACCTC 
AAAATGGACG CGCTGCAAGA CTTTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OT_ostta08g02510 ( XM_022983593.1 ) cDNA ORF clone, Ostreococcus tauri -> XP_022840996.1 Ostreococcus tauri Carbamoyl-phosphate synthase large subunit,CPSase domain (OT_ostta08g02510), partial mRNA.

Price & Availability↑

CloneID	OOd76309
Clone ID Related Accession (Same CDS sequence)	XM_022983593.1
Accession Version	XM_022983593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509984000000
Organism	Ostreococcus tauri
Product	Carbamoyl-phosphate synthase large subunit,CPSase domain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_014433). COMPLETENESS: incomplete on both ends.

ATGTCGTCCA CGCGACGCGC GATCGACGGC GCGCGAGGGG GGATGTGCGG CGGACGCGTC 
ACCGCGACGG CGACGCGAGG GCGACGAGTG ACGCGCCCGG CGCGGTGGAC GACGACGCGG 
GCGGAGAGCG AGGTTTTTCG AGCGTGGGAT CAGGCGACGA AGAGCGCGAT GCGGACCGAT 
CTGAAGAAGA TTATGATTCT CGGTGCGGGA CCGATCGTTA TCGGACAGGC GTGCGAGTTT 
GATTACTCCG GTACCCAGGC GTGCAAGGCG CTGCGAAGCT TGGGATACGA CGTCGTCCTC 
GTGAACTCCA ACCCGGCGAC GATCATGACG GATCCGGAGA CTGCGTCTCG TACGTACGTT 
ACTCCGATGA CTCCGGCGAG CGTCGAGCAA ATCATCGCCA AGGAAAAGCC GGACGCTTTG 
TTGCCAACCA TGGGTGGCCA AACCGCGCTC AACTTGACAA AGGCGCTCGC CGAGTCGGGG 
ATCTTGGAGA AGCACAACGT TGAACTCATC GGCGCTAAGC TCGATGCGAT CAATAAGGCT 
GAAGACCGTC AGCTTTTCAA GAATGCCATG GACAAAATTG GCCTGAGCAC GCCCAAGTCT 
GGCACGGCTG AGACGTGGGA GCAGGCGAAA GCCATCGCCG CGGACATCGG TACGCTCCCG 
CTCATCATTC GTCCGGCGTT CACTCTCGGT GGCTCCGGTG GTGGTATCGC GTACAACATG 
GATGAATTCG AAACGATCGT CAAGGGTGGG CTCGATGCGT CCGCGACGAG CCAAGTTTTG 
ATTGAGCAGT CTTTGCTCGG CTGGAAGGAG TACGAGCTCG AGGTGATGCG CGACCTTGCC 
GACAACGTCG TCATTGTGTG TTCAATTGAA AACTTTGATC CGATGGGCGT GCACACTGGT 
GATTCGATCA CCATCGCACC GGCTCAAACG CTCACCGACA AGGAATACCA GCGTCTTCGT 
GATGCCTCGG TTGCCATCAT CCGTGAGATT GGCGTCGAGT GTGGTGGTTC CAACGTTCAG 
ATGGCTGTCA ACCCGGTCGA CGGCCAGGTC ATGATCATTG AAATGAACCC GCGTGTGTCT 
CGCTCGTCTG CTCTTGCGTC CAAGGCGACC GGTTTCCCGA TCGCTAAGAT GGCCGCGAAG 
CTCGCTGTCG GTTGCACTCT CGACGGTATC CCTAACGACA TCACACTCAA GACCCCGGCG 
TCTTTCGAGC CTTCCATCGA TTATGTCGTC ACGAAGATTC CTCGATTCGC TTTCGAAAAG 
TTCCCGGGCG CGAAGCCCGT GCTTACGACA CAGATGAAGT CTGTCGGTGA GGCCATGGCC 
ATGGGTCGCA CTTGGCAAGA GTCTTTCCAA AAGGCGTTCC GTTCTCTCGA AACCGGCTTC 
GCCGGTTGGG GTCTCGGTAA GAAGGATGGG TTCATGCAAG GTGACGTTGC TGCGATCAAC 
GAGGGCCTAC TCGTGCCGAA CCCCGAGCGC ATTGTTGTCA TTCATGAAGC GTTCCTCGCG 
GGTTTCACGG AGGATCAGAT CATCAAGGCA ACGACGATGG ACCCATGGTT CATCCGACAG 
CTCGGTGAAC TCTACCAGAC CGAGTGCTGG CTCAAATCGC TCAAGTCACT CGACGATCTT 
TCCGAGAACG ATTGGTTCGA AGTTAAGAGG CGTGGCTACA GCGATTCACA AATTTCCATG 
GCTTTCCCGG GCACGACTGA GTTGCAAGTT CGCAAGGAGC GCACGGCGAA GGGTTGCAAG 
CCCTCCATGA AGCGCGTGGA TACGTGCGCG GCCGAATTCG TTGCGGACAC TCCGTATATG 
TATTCCTCGT ACGACGGCTT CGACGAATCT GAACCGACGG ACAACAAGAA AATCCTCATT 
CTGGGTGGTG GTCCGAACCG CATCGGTCAA GGTATTGAGT TCGACTACTG CTGCTGCCAT 
GCCGCGTTCG CCCTCGCGGA CGCTGGTTAC GAAACCATCA TGCTCAACAG TAACCCGGAG 
ACTGTGTCTA CCGACTACGA TACCTCTGAT CGCCTGTACT TCGAACCGCT GACGGTTGAA 
GATGTCTTGA ATGTGTGCGA AGTTGAGCGT CCGGAGGGCA TCATCGTTCA GTTCGGTGGC 
CAAACTCCTT TGAGCCTCGC GACAAGGCTT GAAGCTGCCC TCAAGGAGAA CCCGATTCCG 
GCGGCGTCCG GCAACGGTTT TACACAGATT TTGGGTACCC CTCCGGACTC AATCGATGCT 
GCAGAAGATC GCGAGCGCTG GATCGATATT TTGGAAGAAC TCAACATTCT TCAACCGCCG 
GGAGGTGTTG CGAGATCTGA GGAAGAAGCG TTGAAGATTG CTGAAAAACT TACCTACCCT 
GTCATGGTTC GTCCGTCTTA CGTCCTCGGT GGGCGCGCCA TGGAGATCGT TGGCTCTAAC 
GCCGACTTGA AGCGTTACAT CAAGACTGCC GTCGAAGTCG ACGAAGATCG CCCGGTGCTT 
GTCGATAAGT ACCTCCAAAA CGCGACCGAG ATCGATTGCG ATGCATTGTG CGATAAGGAT 
GGCAACGTTG TCATCGGTGG TATCATGGAA CACATTGAGC AAGCGGGAGT GCACTCTGGT 
GACTCTGCGT GCTCCTTACC AACGCAAACT ATCCCTGAAT CTGCCCTTGC TACCATTCGT 
GAATGGACCC CGAAGCTCGC GAAACGACTC GGTGTCATCG GTCTCATCAA CATTCAGTAC 
GCTGTGACCC CGGAGGGTAC GCCGTACATC ATTGAGGCGA ACCCGCGTGC TTCTCGTACT 
GTGCCGTTCG TGGCGAAGGC GATTGGTCAT CCGTTGGCTA AGTACGCGTC GTTGGTGATG 
GCTGGAAAGA CGCTCAAGGA AATCGGCTTC ACGGAAGAAG TCAAATTGAA CCACGTCGCC 
GTGAAGGAAG CGGTCCTTCC GTTCGACAAG TTCCCGGGTG CGGACACTTT GCTTGGTCCG 
GAGATGAGAA GTACTGGTGA GGTGATGGGC ATTGATCGTG ACTTCAGCCG TGCTTATTGC 
AAGGCCCAGC TCGCTGCGGG ACAGCGACTG CCCACCTCCG GCAAGGTCTT CATTTCCGTG 
CGCGATTCTG ACAAGGACGC CATAGTTGAC ATAGCTCGTG ACTTCGTCAG CATGAACTAC 
ACGGTCGTGT CGACCGGTGG TACGGCGACT CACTTAGAAA AGGCTGGTGT TGCTGTCACT 
AAGGTGCTCA AGTTGCACGA AGGCCGACCG AACGTCTCCG ACATGATTCG TGATGGTGAA 
ATTGGTATGA TGGTGGTTAC CTCGTCTGGT GACGCCCAAG ATTTGGTGGA TGGCCGCAAG 
ATCCGTCGCG AGGCGGTTGG CCTGAAGGTG CCGCTTGTCA CGACCATTGC CGGCGCGAAG 
GCCACGGCCG GTGCGGTTCG CGTCTTGCAG AAGGGTGACC TCAAAATGGA CGCGCTGCAA 
GACTTTTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022840996.1
CDS	1..3432
Translation	MSSTRRAIDGARGGMCGGRVTATATRGRRVTRPARWTTTRAESEVFRAWDQATKSAMRTDLKKIMILGAGPIVIGQACEFDYSGTQACKALRSLGYDVVLVNSNPATIMTDPETASRTYVTPMTPASVEQIIAKEKPDALLPTMGGQTALNLTKALAESGILEKHNVELIGAKLDAINKAEDRQLFKNAMDKIGLSTPKSGTAETWEQAKAIAADIGTLPLIIRPAFTLGGSGGGIAYNMDEFETIVKGGLDASATSQVLIEQSLLGWKEYELEVMRDLADNVVIVCSIENFDPMGVHTGDSITIAPAQTLTDKEYQRLRDASVAIIREIGVECGGSNVQMAVNPVDGQVMIIEMNPRVSRSSALASKATGFPIAKMAAKLAVGCTLDGIPNDITLKTPASFEPSIDYVVTKIPRFAFEKFPGAKPVLTTQMKSVGEAMAMGRTWQESFQKAFRSLETGFAGWGLGKKDGFMQGDVAAINEGLLVPNPERIVVIHEAFLAGFTEDQIIKATTMDPWFIRQLGELYQTECWLKSLKSLDDLSENDWFEVKRRGYSDSQISMAFPGTTELQVRKERTAKGCKPSMKRVDTCAAEFVADTPYMYSSYDGFDESEPTDNKKILILGGGPNRIGQGIEFDYCCCHAAFALADAGYETIMLNSNPETVSTDYDTSDRLYFEPLTVEDVLNVCEVERPEGIIVQFGGQTPLSLATRLEAALKENPIPAASGNGFTQILGTPPDSIDAAEDRERWIDILEELNILQPPGGVARSEEEALKIAEKLTYPVMVRPSYVLGGRAMEIVGSNADLKRYIKTAVEVDEDRPVLVDKYLQNATEIDCDALCDKDGNVVIGGIMEHIEQAGVHSGDSACSLPTQTIPESALATIREWTPKLAKRLGVIGLINIQYAVTPEGTPYIIEANPRASRTVPFVAKAIGHPLAKYASLVMAGKTLKEIGFTEEVKLNHVAVKEAVLPFDKFPGADTLLGPEMRSTGEVMGIDRDFSRAYCKAQLAAGQRLPTSGKVFISVRDSDKDAIVDIARDFVSMNYTVVSTGGTATHLEKAGVAVTKVLKLHEGRPNVSDMIRDGEIGMMVVTSSGDAQDLVDGRKIRREAVGLKVPLVTTIAGAKATAGAVRVLQKGDLKMDALQDFF

Target ORF information:

RefSeq Version	XM_022983593.1
Organism	Ostreococcus tauri
Definition	Ostreococcus tauri Carbamoyl-phosphate synthase large subunit,CPSase domain (OT_ostta08g02510), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022983593.1

ORF Insert Sequence:

ATGTCGTCCA CGCGACGCGC GATCGACGGC GCGCGAGGGG GGATGTGCGG CGGACGCGTC 
ACCGCGACGG CGACGCGAGG GCGACGAGTG ACGCGCCCGG CGCGGTGGAC GACGACGCGG 
GCGGAGAGCG AGGTTTTTCG AGCGTGGGAT CAGGCGACGA AGAGCGCGAT GCGGACCGAT 
CTGAAGAAGA TTATGATTCT CGGTGCGGGA CCGATCGTTA TCGGACAGGC GTGCGAGTTT 
GATTACTCCG GTACCCAGGC GTGCAAGGCG CTGCGAAGCT TGGGATACGA CGTCGTCCTC 
GTGAACTCCA ACCCGGCGAC GATCATGACG GATCCGGAGA CTGCGTCTCG TACGTACGTT 
ACTCCGATGA CTCCGGCGAG CGTCGAGCAA ATCATCGCCA AGGAAAAGCC GGACGCTTTG 
TTGCCAACCA TGGGTGGCCA AACCGCGCTC AACTTGACAA AGGCGCTCGC CGAGTCGGGG 
ATCTTGGAGA AGCACAACGT TGAACTCATC GGCGCTAAGC TCGATGCGAT CAATAAGGCT 
GAAGACCGTC AGCTTTTCAA GAATGCCATG GACAAAATTG GCCTGAGCAC GCCCAAGTCT 
GGCACGGCTG AGACGTGGGA GCAGGCGAAA GCCATCGCCG CGGACATCGG TACGCTCCCG 
CTCATCATTC GTCCGGCGTT CACTCTCGGT GGCTCCGGTG GTGGTATCGC GTACAACATG 
GATGAATTCG AAACGATCGT CAAGGGTGGG CTCGATGCGT CCGCGACGAG CCAAGTTTTG 
ATTGAGCAGT CTTTGCTCGG CTGGAAGGAG TACGAGCTCG AGGTGATGCG CGACCTTGCC 
GACAACGTCG TCATTGTGTG TTCAATTGAA AACTTTGATC CGATGGGCGT GCACACTGGT 
GATTCGATCA CCATCGCACC GGCTCAAACG CTCACCGACA AGGAATACCA GCGTCTTCGT 
GATGCCTCGG TTGCCATCAT CCGTGAGATT GGCGTCGAGT GTGGTGGTTC CAACGTTCAG 
ATGGCTGTCA ACCCGGTCGA CGGCCAGGTC ATGATCATTG AAATGAACCC GCGTGTGTCT 
CGCTCGTCTG CTCTTGCGTC CAAGGCGACC GGTTTCCCGA TCGCTAAGAT GGCCGCGAAG 
CTCGCTGTCG GTTGCACTCT CGACGGTATC CCTAACGACA TCACACTCAA GACCCCGGCG 
TCTTTCGAGC CTTCCATCGA TTATGTCGTC ACGAAGATTC CTCGATTCGC TTTCGAAAAG 
TTCCCGGGCG CGAAGCCCGT GCTTACGACA CAGATGAAGT CTGTCGGTGA GGCCATGGCC 
ATGGGTCGCA CTTGGCAAGA GTCTTTCCAA AAGGCGTTCC GTTCTCTCGA AACCGGCTTC 
GCCGGTTGGG GTCTCGGTAA GAAGGATGGG TTCATGCAAG GTGACGTTGC TGCGATCAAC 
GAGGGCCTAC TCGTGCCGAA CCCCGAGCGC ATTGTTGTCA TTCATGAAGC GTTCCTCGCG 
GGTTTCACGG AGGATCAGAT CATCAAGGCA ACGACGATGG ACCCATGGTT CATCCGACAG 
CTCGGTGAAC TCTACCAGAC CGAGTGCTGG CTCAAATCGC TCAAGTCACT CGACGATCTT 
TCCGAGAACG ATTGGTTCGA AGTTAAGAGG CGTGGCTACA GCGATTCACA AATTTCCATG 
GCTTTCCCGG GCACGACTGA GTTGCAAGTT CGCAAGGAGC GCACGGCGAA GGGTTGCAAG 
CCCTCCATGA AGCGCGTGGA TACGTGCGCG GCCGAATTCG TTGCGGACAC TCCGTATATG 
TATTCCTCGT ACGACGGCTT CGACGAATCT GAACCGACGG ACAACAAGAA AATCCTCATT 
CTGGGTGGTG GTCCGAACCG CATCGGTCAA GGTATTGAGT TCGACTACTG CTGCTGCCAT 
GCCGCGTTCG CCCTCGCGGA CGCTGGTTAC GAAACCATCA TGCTCAACAG TAACCCGGAG 
ACTGTGTCTA CCGACTACGA TACCTCTGAT CGCCTGTACT TCGAACCGCT GACGGTTGAA 
GATGTCTTGA ATGTGTGCGA AGTTGAGCGT CCGGAGGGCA TCATCGTTCA GTTCGGTGGC 
CAAACTCCTT TGAGCCTCGC GACAAGGCTT GAAGCTGCCC TCAAGGAGAA CCCGATTCCG 
GCGGCGTCCG GCAACGGTTT TACACAGATT TTGGGTACCC CTCCGGACTC AATCGATGCT 
GCAGAAGATC GCGAGCGCTG GATCGATATT TTGGAAGAAC TCAACATTCT TCAACCGCCG 
GGAGGTGTTG CGAGATCTGA GGAAGAAGCG TTGAAGATTG CTGAAAAACT TACCTACCCT 
GTCATGGTTC GTCCGTCTTA CGTCCTCGGT GGGCGCGCCA TGGAGATCGT TGGCTCTAAC 
GCCGACTTGA AGCGTTACAT CAAGACTGCC GTCGAAGTCG ACGAAGATCG CCCGGTGCTT 
GTCGATAAGT ACCTCCAAAA CGCGACCGAG ATCGATTGCG ATGCATTGTG CGATAAGGAT 
GGCAACGTTG TCATCGGTGG TATCATGGAA CACATTGAGC AAGCGGGAGT GCACTCTGGT 
GACTCTGCGT GCTCCTTACC AACGCAAACT ATCCCTGAAT CTGCCCTTGC TACCATTCGT 
GAATGGACCC CGAAGCTCGC GAAACGACTC GGTGTCATCG GTCTCATCAA CATTCAGTAC 
GCTGTGACCC CGGAGGGTAC GCCGTACATC ATTGAGGCGA ACCCGCGTGC TTCTCGTACT 
GTGCCGTTCG TGGCGAAGGC GATTGGTCAT CCGTTGGCTA AGTACGCGTC GTTGGTGATG 
GCTGGAAAGA CGCTCAAGGA AATCGGCTTC ACGGAAGAAG TCAAATTGAA CCACGTCGCC 
GTGAAGGAAG CGGTCCTTCC GTTCGACAAG TTCCCGGGTG CGGACACTTT GCTTGGTCCG 
GAGATGAGAA GTACTGGTGA GGTGATGGGC ATTGATCGTG ACTTCAGCCG TGCTTATTGC 
AAGGCCCAGC TCGCTGCGGG ACAGCGACTG CCCACCTCCG GCAAGGTCTT CATTTCCGTG 
CGCGATTCTG ACAAGGACGC CATAGTTGAC ATAGCTCGTG ACTTCGTCAG CATGAACTAC 
ACGGTCGTGT CGACCGGTGG TACGGCGACT CACTTAGAAA AGGCTGGTGT TGCTGTCACT 
AAGGTGCTCA AGTTGCACGA AGGCCGACCG AACGTCTCCG ACATGATTCG TGATGGTGAA 
ATTGGTATGA TGGTGGTTAC CTCGTCTGGT GACGCCCAAG ATTTGGTGGA TGGCCGCAAG 
ATCCGTCGCG AGGCGGTTGG CCTGAAGGTG CCGCTTGTCA CGACCATTGC CGGCGCGAAG 
GCCACGGCCG GTGCGGTTCG CGTCTTGCAG AAGGGTGACC TCAAAATGGA CGCGCTGCAA 
GACTTTTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.
BMC genomics151103(2014 Dec)
Blanc-Mathieu R,Verhelst B,Derelle E,Rombauts S,Bouget FY,Carré I,Château A,Eyre-Walker A,Grimsley N,Moreau H,Piégu B,Rivals E,Schackwitz W,Van de Peer Y,Piganeau G

Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features.
Proceedings of the National Academy of Sciences of the United States of America103(31)11647-52(2006 Aug)
Derelle E,Ferraz C,Rombauts S,Rouzé P,Worden AZ,Robbens S,Partensky F,Degroeve S,Echeynié S,Cooke R,Saeys Y,Wuyts J,Jabbari K,Bowler C,Panaud O,Piégu B,Ball SG,Ral JP,Bouget FY,Piganeau G,De Baets B,Picard A,Delseny M,Demaille J,Van de Peer Y,Moreau H

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OT_ostta08g02510 cDNA ORF clone, Ostreococcus tauri

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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