PAAG_02977 cDNA ORF clone, Paracoccidioides lutzii Pb01

Gene
Clones
gRNAs

Summary

Gene Symbol	PAAG_02977
Entrez Gene ID	9097920
Full Name	mycocerosic acid synthase
Gene Type	protein-coding
Organism	Paracoccidioides lutzii Pb01

mRNA and Protein(s)

mRNA	Protein	Name
XM_015844808.1	XP_015702014.1	mycocerosic acid synthase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis.
Desjardins CA, Champion MD, Holder JW, Muszewska A, Goldberg J, Bailão AM, Brigido MM, Ferreira ME, Garcia AM, Grynberg M, Gujja S, Heiman DI, Henn MR, Kodira CD, León-Narváez H, Longo LV, Ma LJ, Malavazi I, Matsuo AL, Morais FV, Pereira M, Rodríguez-Brito S, Sakthikumar S, Salem-Izacc SM, Sykes SM, Teixeira MM, Vallejo MC, Walter ME, Yandava C, Young S, Zeng Q, Zucker J, Felipe MS, Goldman GH, Haas BJ, McEwen JG, Nino-Vega G, Puccia R, San-Blas G, Soares CM, Birren BW, Cuomo CA
PLoS genetics7(10)e1002345(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PAAG_02977 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAAG_02977 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo123508	XM_015844808.1 Latest version!	Paracoccidioides lutzii Pb01 mycocerosic acid synthase (PAAG_02977), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAAG_02977 ( XM_015844808.1 ) cDNA ORF clone, Paracoccidioides lutzii Pb01 -> XP_015702014.1 Paracoccidioides lutzii Pb01 mycocerosic acid synthase (PAAG_02977), partial mRNA.

Price & Availability↑

CloneID	OPo123508
Clone ID Related Accession (Same CDS sequence)	XM_015844808.1
Accession Version	XM_015844808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1507564800000
Organism	Paracoccidioides lutzii Pb01
Product	mycocerosic acid synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_015440954). COMPLETENESS: incomplete on both ends.

ATGAATCCAG AAAAAGGGGC GGTTGTGGGC AAGTGTAGTG GGGGCAATTT GATGCCATTG 
GCTATTGTTG GCATGTCCTG TCGTCTTTCG GGAGATGTCA CCAGCCCTGA TACTCTCTGG 
GAGTTGTGCT CTCGTCGGCG AAGTGGTTGG TCCACGATCC CAGAGCATCG ATTCAATGCT 
GCGGCTTTCT ACCATCCCAA TCCGGATAGA AATGGAGCTT TCAACTTTAA TGGCGGCTAC 
TTTCTCAAGG AAGACATCGG TCTCTTTGAT GCGCCATTCT TTAATCTCAC CAAGCACGAG 
GCAGAGTCCA TTGACCCACA GCAACGTCTG ATGCTTGAAT GCACCTACGA GGCGCTTGAG 
AATGGAGGTA TTACATTGGA ATCACTGGCT GATGATCAGG TGGGCGTCTT CGTTGGCGCA 
TCGATCTCGG ATTACGATTT GAACAATTAC AAGGATAGCG AAAACATTTC ACGGTACAAC 
GCAACCGGAT GCGCGTTGTC ACTTCAATCC AACAGAATTT CATACTATTT CAACTTCAAA 
GGTCCAAGTA TTACCATTGA TACTGCATGC TCGTCTAGCT TGACCGCATT ACATGTCGCC 
TGTCAAAGCC TTAGATCACG GGAATCGACC TGCGCAATTG TCGGCGGTTG CCATCTAAAC 
CTGTTACCGG ATGTGTTCAT CTCCATGTCT CTCCAAAGGC TTTTCTCTAA TGCTGGAAAA 
TGTTACCCAT TTGATCATAG AGCGCAGAGT GGATTTGGAC CTGGAGAGGG TGCAGCGTGT 
ATTGTCCTCA AACCGTTGGA TGTTGCGATT GAAGCCGGCG ACTCAATAAG GGCTGTAATA 
GTCAACAGTG GGATAAATCA AGACGGGCGG ACCCAGGGAG TTACGATGCC GAATATTGCC 
GCTCAGGAAC AACTTATTAG ATCGGTTTAT GCGCAAGCTC ACATAGACCC TATAGATACC 
GGATACGTGG AAGCGCATGG GACTGGCACC AAAGTTGGCG ACCCGATAGA GGCGAGAGCG 
CTTCATACTG TTTTCGGCAA AGGAAGGCCG TCGATGAAAC CTTTGTTTGT GGGCTCACTT 
AAATCTAACG TCGGGCATAC TGAAGGGGCA AGTGGAGTGG TTTCAGTAAT AAAAACGGCA 
ATGATGCTTG AGAAGGGTTT TATTTTACCG AACTGTGATT TCGAGAAACC GAATAATGCT 
ATCCCATTGG AGGAATGGAA TTTAAAGGTG CCCAAAAGAC AAGTGCCATG GCCCCGCGAG 
AAGACTTACG CCAGCGTCAA TAATTTTGGA TTTGGAGGAT CAAATGCCCA TGTCATTCTA 
AAGAAGGCCC CGCCTCTATC AGAAGTTGCC TGGAAAGCCC GATCCGATCT TGAGACGCCA 
GCACCAAAAC CTTCCCTTCT GCGCAAGGTT TATACTCTGT CCTCACATAA CAGGGGGGGC 
TTATCTCAGC AGATAAAGGC CCTTGAAATA TACTTAGAGA AGCATCCAGA AGCTTTCGAT 
CTTCACTTGA TGGACAACTT GGCCTACACA CTTTGTCAGC GACGAAATCA CCTTCCGTGG 
AGAGTTGCAG TTTCGGCTCC GTCTAGCACA CAGCTGATAG AAGTTCTGTC TACAAATATC 
ACACCCAAGC GGTCTGCAGG GGAGCAAGTA ATCGGATTTG TTTTTACTGG CCAGGGTGCC 
CAATGGAATG CCATGGGTAG AGAGCTCCTT GTTGCCTATC CGATATTTAG AGCCACAATG 
GAAGCGGCAG ATCAGTGCCT GGTTAAACTA GGTGCTATGT TTTCCTTGAT CGATGAGTTA 
TCCAAGGATA CTGGTACAAG TTTTATTTAT GAAGCATGTT TCAGTCAGCC CTCTTGTACA 
GCGATTCAGA TAGCTTTGAC AGAGCTTCTA AGTTCGTGGG GAATACAGCC TGTTTCCGTG 
GTTGGCCACT CTAGCGGTGA GATTGCTGCG GCGTATGCTG CCGGTATGCT TTCGCTCGAA 
GATTGCATCA CCATTGCCTA CACTCGGGGA ACCGTAGCAG ACCAAATATC GAAGGAATTT 
CCAGAAGTCA AAGGAGCCAT GTTGGCCGTT GGGGCGGGTG CATCGGATAT AGGCCCTCTT 
CTTGACACTC TTCAAGGAGG AAGTGCAGTA ATTGCCTGCG TCAATAGTCA AAAGAGTGTT 
ACGGTCTCTG GCGATGAAAC GGCAATAAGT GAATTGCAAA GCAAGGTTGA CGATCAAGGA 
ATTTTCAATA GAAAACTCCC AGTGAACGTA GCATATCACT CCTATCATAT GAGACTAGTT 
TCGGAAAAAT ATCGGTCGAT GATGGGAAAC ATCGTCCCCA AATCCGCAGC CATCAAATTC 
CACTCATCAG TGTTCGGTCA CCAAGCCACA TATTCCGATC TTACCGGTGA TTATTGGGTA 
GACAACTTCG TATCTCCTGT CGAGTTTGTA CGCGGCTTAC AAAGCCTCCT GTTGGAAAGT 
CGAGCTACCT CTGGCAAACA AATAAATATG CTTGTTGAGA TTGGACCACA CCCAGCACTG 
AAGGGCCCAA TCCGAGATAT TCTACAGCAT GATTCCCTGG GTGGAGAGAT AAAATATCTG 
CATAGTCTTA AACGCGAACA AGACGCCGTT GAGGCAATGC AGGCACTGGG GACGGAGTTA 
TTCATGAATG GGGCTAAACT CAACTTCAAC TCCATCAACT TTCCATCCGA CAAAGGAAAG 
AGACCTGTCT TGCTAACGAA TCTGCCGAGT TATCCTTGGA ACCACACTAC AAAATATTGG 
CAAACCTCCC GTCTGTCTCA AAATCACTGT CATAAGCCTT TTCCACGAAA CGATATTCTT 
GGCTCACTCT CTGTAGAAAG CGATGATATA GAACCCAGAT GGAGAAACGT TCTGCGGGCA 
GATGACCATC CATGGATTCG ACATCATAAA GTTCAAGGAA ACAATGTATA TCCTATGACT 
GGTTACCTTG CCATGGTGCT GGAAGCCGCA ACCCAGCATT CCATTATGAA AAAGGTAGAG 
TTCGATCACT TTGTATTTCG GGAAGTGTCT ATTGGGCGAG CCCTGATTAT TTCGGACACG 
TCTGCAGTCG AAACTATGAC TACTCTAAGA CCGTACGCTG AGGGCACTAG AATATCGTCG 
GATGCTTGGA GTGAATTTCG AGTATTTTCA TGGTCGGATA CTAAAGGATG GGATGAGCAT 
TGTCGAGGAC TTGTGAAGGT TTATAAAATC GGGGGCCCAA ACCCCATTGA TGGCTCTTAT 
CAGCACAGAG AGAACTCCCT TTATATCAAA GAGCAGGTAG CTGCATTTCA CTCCAATTGT 
GCATCTACCC CTGATGTCCA TGAAATGTAT GACGCTGTTG CTGCCAATGC TTTGGAGTAT 
GGCCCGTTAT TTCAAGCAGC ATCGAAAGTT TTCGTCGGTA ATCAACAAGC GATATTCGAC 
ATATCGGTGA CTGACACAAG AGGTTCTATG CCTCAAGAGA CTGAAACCGA CTTGATCATT 
CATCCTGCGG CTTTAGATGC GTGTGTGCAA ATTGTATGGC CTCTCTTGGG ATTTACTAAC 
CCGGGGCCGC ATTCCCTTTA CCTACCATCC TTCTTAAAAT GCCTGTCTGT TCCCTGCTCA 
ACGTCCATGC CAATTGGAGG CCAGTTTCGG GTCTATGGGA GTCGATTGAA CTCACTCTCT 
CATGGCGTTC CAATCAACCA CGATATATTT GTTACCTCCG CTGAAAATCC CACCGAAATT 
CTCTTAAAAA TGGAGGGGTT AACGTTAACA CCTGTTCATG ATGACATAGC GTCTGCGAAT 
GGTGAGCCCA AAGTGTTATG CTATAAGCTT TGCTTAGAGC CCTGCCTAGA GTTTTTGACC 
ACGGAAAATT TGAGATCATT TGATGGGGCT CATAACACGA ACCAGAACGA AAAGAGCCAT 
GTTCACATCC TCAATCAGGT TTCGTTTTAT TTCCTCGAGA AAGCGTTAAA ACAGCTTGAC 
AAAGAGGAAT ACCGTAGCCT CCGTCAGAAA CACGAGCCGT TCTATCAGTG GATACGAACT 
TTGAACTTAC AGACAGAGAA TGGAAGAATT AGACTTCATC ACTCAGATTG GTCTCAATTC 
AACGAGGAAA GGTGGTCATT CGTTATTAAC ATGGCTCGGT GTATGGGGAC AGTGGGGAAA 
GTGACATGTT CCATTGGTGA AAATTTTGTA CCGATTTTAA ATCGGGGAAT AGCCTCAATA 
TCTACCAGTC TTGGTGACGA TCTACTGGAA CAATTCTACG ATGAGCTTAG TCCCCTTAAC 
CGGAATTACG CCAACGCCGC CATTTGTATC GACAAGATGG CTTTCCAGTA CCCTGACATG 
GAAATTCTTG AGATCGGAGC TGGCACAGGG GAAGGATCTT TACGCATACT GCGAATTTTG 
GGGGGTGGTG ACACTGGAAA AATGCGGAGA TTCGCCAAAT ACACGTACAC TGCTAAAAGC 
GCCCATACAC TCGTTAAAGC TAAGCCTAAG TTTCAAGCCT GGGGTAGGTT CGTGTCATGT 
CGAAACTTGA ATATTTCGCA AGATCCTTCT GGCCAAGGTT ATTTACCAAA ATCGTACGAT 
CTAATAATCG CTTGTCATTT ATCGGATTCG ACAGAATGTT TACATGAAGC CATGGCCAAT 
GTGAATGGAC TTCTGAAACC TGGAGGGAAA CTGGTTTTGC TCGATTGGAC GGCCAGACAT 
TTATATCAGT TCGTTCCCAA ATTGCTTTCA GGCTCTTGGT CAACCGAAAG ATCAGGTCGT 
GCGGGTAGTT CTATTCGGAA TAAAGACGAG TGGGACAAGC TGTTCCGAGA AACAAAGTTT 
TCTGGATTAG ACCTATCTCT GGACGACTAT CCAGAGACCC TTGAGCAGTC CAGTAGTCTG 
ATGCTATCAA CCGTCCAAGC ATCCGATGAA TTATCCGGCA AGGAAATAGC TCTAGTTTAC 
CACGGGGGAG TCAATGACTC TCTCGCACCT GGCCTTATCC GCGAGTTGGA AAATATAACT 
GGCATCCGCC CCTCCGTTGA AGAACTCTGC CAAGTTAATG CAAAAGGTAG ACTATGCATT 
TTTTTCGGCG AACTATATCA GCCGCTTTTA TCCAGCATGA CACCGGATCT TCTTTCAGCA 
ATTCAACTTC TAGTATCTAG TGCCAGTGGT ATTCTCTGGG TCATTCGTCA AGACCACCTA 
GTCTCGGGCT CACCTGAATC CAGCATGGCG ATCGGGCTAG CCAGAACATT ACGCTCAGAA 
ACGGCGATAC AACTTGCGAC TCTAGAGCTG GAAGGCAATC CCCACTATGG CGAAGCTGAC 
CAAATAATGA AGGTCTTCAG AGCTGTATTT GGTACTGAAC CTAGTCTCAG GCAAGGAGAT 
ATGGAATATG TGGTTAAAAA TAGACTTATT TGTGTTTCGC GCGTTTTTGC AGATCCAGAA 
ACGAACAAAT ATGTTCACGA AGAGTCACAC AGAATCCCCC CTGAACTACA GCCTTTCTAT 
CAAAACAACA GGCGCCTCAA ACTAGCGATG GGACATCATG GCACTCTGGA CAGTTCTTAT 
TTTACGGATG AGCTCTCAAC AACTCAGGTC CCAGCCAATT GGATAGAAAT CGAAACTCGA 
TTTATAGGAC TAAATTTCAA AGATATTATG ATTGCCATGG GTCAACTCCA AAACGGTCAT 
TTAGGGAATG AATGTAGCGG AGTCGTTACC GCGATTGGGG ATAAAGTGAC GGACTTCAAA 
GTTGGCGACC GGATTTGTGC CGTCTCTGAG GGAGCTTTTG CCAATTACGC TCGCTTTCCG 
GCATCAAGCG CCTGGAAGAT TCCAGATAAG ATGAGTTTTG ATGTAGCAGC ATCCATTCCA 
ATTGTATTTT GCACAGCGTA TTACTCTTTG TTTGATGTTG GTCGTCTGCA ACGGGGGGAG 
CGGATTCTCC TTCATGCCGC CGGAGGAGGG GTAGGCCAAG CGGCCATTAT CCTCGCTCAA 
TCGATTGGTG CGGAGATTTA TGCAACTGTT GGTAGTGCTG ATAAGAAGAA GTTTCTAATG 
GAAACCTACA ACATCGACGA AACAAGAATC TTTTTCAGTC GGGATATTTC TTTCGCTGAC 
AGAATCCGCA AAGCCACGGA TGGAGCAGGC GTTGATGTTG TCTTGAATTC TCTTGCTGGT 
GAAATGCTAC AGGCAACTTG GGAATGCATT GCTCCGTTTG GGCGATTCAT AGAAATTGGT 
AAAAAGGACA TTGTACGGAA TTCGAGACTT GAAATGATCC ACTTCGACCG GAACGTAAGT 
TTTGCATCTG TTGATTTGAC ACTCTTGGCC AACAAACGCC CGCAGTTGAT GAAAAAAGTT 
CTAGCTGGAG TCTTTGGGAT GTTTGAAAAT GGCAGTGCTA GGAGTATATC GCCAATTACG 
CGCTTTTCAA TATCGGATTT CGAGAAGGCG TGTCATAATT TACAGACTGG CCGAAGTATC 
GGAAAGATTG TCATCGAAGC GGGGAAGAAT GCTTGCGTCA AGGTTTATCC AAGAAAGAAG 
CAAGGAGATA TTCTCTTTGC GGAAGCCTCC TACATAGTCG TGGGTGGTAC GGGAGGGCTC 
GGACGAAATA TTACGTCATG GTTAGCTGAG AAAGGGGCGC GACATATCAT TGTCGTCTCA 
CGAAGCGGTG CCTCGGATGA AAGAGTTGGG GAGATGATTA AGGATCTCGC TGCAGACGGA 
GCAACTGTCG TTGTATGCCA ATGCGATATC AGCAAAAAAG AGGAAGTCGA AACCAAACTG 
GCAACAATAC TACCGCAAGC GCCACCTGTG CGCGGGGTAA TATATTGCGC AATGGTTCTC 
CGGGACACGC TGTTCGAACG GATGACCTTT GATGACTACG ACGGGGTTGT GAAACCAAAG 
GTTGCAGGAG TGTGGAATCT ACACCATGCG CTCGTAGGAT TAAGCTGTGA TCTTGATTTC 
TTCATCACTC TCTCTTCAAT TGCTAGTCTT GTCGGTAACA GGGGACAAGC TGCATATGCC 
GCTGGTGGCA CTTTCATGAC AGCTTTCGCA CAATATGGAA ACGCGCGTGG TTTACCTTGC 
ACTACTATCG ATCTGGCTCC CGTTCAAGGA ATCGGGTATC TCGCGGAAAA TCAGCAGAGG 
AAGAACGAAG TCGAAAACTC GCTAGTCGTG GATTGGGTTA ATGAAACCCA GCTTCGAGGA 
CTTCTTGCCG CCGCCATCAG AGGCGATATG GCCAATACCT GTCACCACCA CTGCATCACC 
GGCCTAGGAA TATTGAAATC CATCTCCGGT AAAGAGACAC CGTGGTGGGC CTTGGATCCA 
AGGTTTTCAC ATCTCGTTCA GTCGTGCGCC TCTGATGAAG GCCAAACATG CGACCCCGCA 
TCTTCTCTAG CCACAGCATC TCCCGGTCTT GCGGTGAAAA ATACAGCTGA TCGCGCAGAC 
GCAGAAAGAA TAGTTACGGA TGCTCTTGTA CAAAAAGTAT CCACCATAAT GATGCGGTCC 
GTCGACGAAA TTGACCCTTC AAAACCTCTG GGCACATACG GGCTGGACTC CCTGATCACC 
ATTGAGGTGC GCAATTGGAT CTCTCGCGAG CTCCAAGCGA GCTTGCAAAT CATGGAGATT 
CTCGTGACCA AGTCTGTGGT AGAGCTGGCG GGGCTGATAT TGAAGAAATC AAAGATCGTG 
AGCGTGGAGG CGAAATCGCG ATGGGGGCTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015702014.1
CDS	1..7656
Translation	MNPEKGAVVGKCSGGNLMPLAIVGMSCRLSGDVTSPDTLWELCSRRRSGWSTIPEHRFNAAAFYHPNPDRNGAFNFNGGYFLKEDIGLFDAPFFNLTKHEAESIDPQQRLMLECTYEALENGGITLESLADDQVGVFVGASISDYDLNNYKDSENISRYNATGCALSLQSNRISYYFNFKGPSITIDTACSSSLTALHVACQSLRSRESTCAIVGGCHLNLLPDVFISMSLQRLFSNAGKCYPFDHRAQSGFGPGEGAACIVLKPLDVAIEAGDSIRAVIVNSGINQDGRTQGVTMPNIAAQEQLIRSVYAQAHIDPIDTGYVEAHGTGTKVGDPIEARALHTVFGKGRPSMKPLFVGSLKSNVGHTEGASGVVSVIKTAMMLEKGFILPNCDFEKPNNAIPLEEWNLKVPKRQVPWPREKTYASVNNFGFGGSNAHVILKKAPPLSEVAWKARSDLETPAPKPSLLRKVYTLSSHNRGGLSQQIKALEIYLEKHPEAFDLHLMDNLAYTLCQRRNHLPWRVAVSAPSSTQLIEVLSTNITPKRSAGEQVIGFVFTGQGAQWNAMGRELLVAYPIFRATMEAADQCLVKLGAMFSLIDELSKDTGTSFIYEACFSQPSCTAIQIALTELLSSWGIQPVSVVGHSSGEIAAAYAAGMLSLEDCITIAYTRGTVADQISKEFPEVKGAMLAVGAGASDIGPLLDTLQGGSAVIACVNSQKSVTVSGDETAISELQSKVDDQGIFNRKLPVNVAYHSYHMRLVSEKYRSMMGNIVPKSAAIKFHSSVFGHQATYSDLTGDYWVDNFVSPVEFVRGLQSLLLESRATSGKQINMLVEIGPHPALKGPIRDILQHDSLGGEIKYLHSLKREQDAVEAMQALGTELFMNGAKLNFNSINFPSDKGKRPVLLTNLPSYPWNHTTKYWQTSRLSQNHCHKPFPRNDILGSLSVESDDIEPRWRNVLRADDHPWIRHHKVQGNNVYPMTGYLAMVLEAATQHSIMKKVEFDHFVFREVSIGRALIISDTSAVETMTTLRPYAEGTRISSDAWSEFRVFSWSDTKGWDEHCRGLVKVYKIGGPNPIDGSYQHRENSLYIKEQVAAFHSNCASTPDVHEMYDAVAANALEYGPLFQAASKVFVGNQQAIFDISVTDTRGSMPQETETDLIIHPAALDACVQIVWPLLGFTNPGPHSLYLPSFLKCLSVPCSTSMPIGGQFRVYGSRLNSLSHGVPINHDIFVTSAENPTEILLKMEGLTLTPVHDDIASANGEPKVLCYKLCLEPCLEFLTTENLRSFDGAHNTNQNEKSHVHILNQVSFYFLEKALKQLDKEEYRSLRQKHEPFYQWIRTLNLQTENGRIRLHHSDWSQFNEERWSFVINMARCMGTVGKVTCSIGENFVPILNRGIASISTSLGDDLLEQFYDELSPLNRNYANAAICIDKMAFQYPDMEILEIGAGTGEGSLRILRILGGGDTGKMRRFAKYTYTAKSAHTLVKAKPKFQAWGRFVSCRNLNISQDPSGQGYLPKSYDLIIACHLSDSTECLHEAMANVNGLLKPGGKLVLLDWTARHLYQFVPKLLSGSWSTERSGRAGSSIRNKDEWDKLFRETKFSGLDLSLDDYPETLEQSSSLMLSTVQASDELSGKEIALVYHGGVNDSLAPGLIRELENITGIRPSVEELCQVNAKGRLCIFFGELYQPLLSSMTPDLLSAIQLLVSSASGILWVIRQDHLVSGSPESSMAIGLARTLRSETAIQLATLELEGNPHYGEADQIMKVFRAVFGTEPSLRQGDMEYVVKNRLICVSRVFADPETNKYVHEESHRIPPELQPFYQNNRRLKLAMGHHGTLDSSYFTDELSTTQVPANWIEIETRFIGLNFKDIMIAMGQLQNGHLGNECSGVVTAIGDKVTDFKVGDRICAVSEGAFANYARFPASSAWKIPDKMSFDVAASIPIVFCTAYYSLFDVGRLQRGERILLHAAGGGVGQAAIILAQSIGAEIYATVGSADKKKFLMETYNIDETRIFFSRDISFADRIRKATDGAGVDVVLNSLAGEMLQATWECIAPFGRFIEIGKKDIVRNSRLEMIHFDRNVSFASVDLTLLANKRPQLMKKVLAGVFGMFENGSARSISPITRFSISDFEKACHNLQTGRSIGKIVIEAGKNACVKVYPRKKQGDILFAEASYIVVGGTGGLGRNITSWLAEKGARHIIVVSRSGASDERVGEMIKDLAADGATVVVCQCDISKKEEVETKLATILPQAPPVRGVIYCAMVLRDTLFERMTFDDYDGVVKPKVAGVWNLHHALVGLSCDLDFFITLSSIASLVGNRGQAAYAAGGTFMTAFAQYGNARGLPCTTIDLAPVQGIGYLAENQQRKNEVENSLVVDWVNETQLRGLLAAAIRGDMANTCHHHCITGLGILKSISGKETPWWALDPRFSHLVQSCASDEGQTCDPASSLATASPGLAVKNTADRADAERIVTDALVQKVSTIMMRSVDEIDPSKPLGTYGLDSLITIEVRNWISRELQASLQIMEILVTKSVVELAGLILKKSKIVSVEAKSRWGLS

Target ORF information:

RefSeq Version	XM_015844808.1
Organism	Paracoccidioides lutzii Pb01
Definition	Paracoccidioides lutzii Pb01 mycocerosic acid synthase (PAAG_02977), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015844808.1

ORF Insert Sequence:

ATGAATCCAG AAAAAGGGGC GGTTGTGGGC AAGTGTAGTG GGGGCAATTT GATGCCATTG 
GCTATTGTTG GCATGTCCTG TCGTCTTTCG GGAGATGTCA CCAGCCCTGA TACTCTCTGG 
GAGTTGTGCT CTCGTCGGCG AAGTGGTTGG TCCACGATCC CAGAGCATCG ATTCAATGCT 
GCGGCTTTCT ACCATCCCAA TCCGGATAGA AATGGAGCTT TCAACTTTAA TGGCGGCTAC 
TTTCTCAAGG AAGACATCGG TCTCTTTGAT GCGCCATTCT TTAATCTCAC CAAGCACGAG 
GCAGAGTCCA TTGACCCACA GCAACGTCTG ATGCTTGAAT GCACCTACGA GGCGCTTGAG 
AATGGAGGTA TTACATTGGA ATCACTGGCT GATGATCAGG TGGGCGTCTT CGTTGGCGCA 
TCGATCTCGG ATTACGATTT GAACAATTAC AAGGATAGCG AAAACATTTC ACGGTACAAC 
GCAACCGGAT GCGCGTTGTC ACTTCAATCC AACAGAATTT CATACTATTT CAACTTCAAA 
GGTCCAAGTA TTACCATTGA TACTGCATGC TCGTCTAGCT TGACCGCATT ACATGTCGCC 
TGTCAAAGCC TTAGATCACG GGAATCGACC TGCGCAATTG TCGGCGGTTG CCATCTAAAC 
CTGTTACCGG ATGTGTTCAT CTCCATGTCT CTCCAAAGGC TTTTCTCTAA TGCTGGAAAA 
TGTTACCCAT TTGATCATAG AGCGCAGAGT GGATTTGGAC CTGGAGAGGG TGCAGCGTGT 
ATTGTCCTCA AACCGTTGGA TGTTGCGATT GAAGCCGGCG ACTCAATAAG GGCTGTAATA 
GTCAACAGTG GGATAAATCA AGACGGGCGG ACCCAGGGAG TTACGATGCC GAATATTGCC 
GCTCAGGAAC AACTTATTAG ATCGGTTTAT GCGCAAGCTC ACATAGACCC TATAGATACC 
GGATACGTGG AAGCGCATGG GACTGGCACC AAAGTTGGCG ACCCGATAGA GGCGAGAGCG 
CTTCATACTG TTTTCGGCAA AGGAAGGCCG TCGATGAAAC CTTTGTTTGT GGGCTCACTT 
AAATCTAACG TCGGGCATAC TGAAGGGGCA AGTGGAGTGG TTTCAGTAAT AAAAACGGCA 
ATGATGCTTG AGAAGGGTTT TATTTTACCG AACTGTGATT TCGAGAAACC GAATAATGCT 
ATCCCATTGG AGGAATGGAA TTTAAAGGTG CCCAAAAGAC AAGTGCCATG GCCCCGCGAG 
AAGACTTACG CCAGCGTCAA TAATTTTGGA TTTGGAGGAT CAAATGCCCA TGTCATTCTA 
AAGAAGGCCC CGCCTCTATC AGAAGTTGCC TGGAAAGCCC GATCCGATCT TGAGACGCCA 
GCACCAAAAC CTTCCCTTCT GCGCAAGGTT TATACTCTGT CCTCACATAA CAGGGGGGGC 
TTATCTCAGC AGATAAAGGC CCTTGAAATA TACTTAGAGA AGCATCCAGA AGCTTTCGAT 
CTTCACTTGA TGGACAACTT GGCCTACACA CTTTGTCAGC GACGAAATCA CCTTCCGTGG 
AGAGTTGCAG TTTCGGCTCC GTCTAGCACA CAGCTGATAG AAGTTCTGTC TACAAATATC 
ACACCCAAGC GGTCTGCAGG GGAGCAAGTA ATCGGATTTG TTTTTACTGG CCAGGGTGCC 
CAATGGAATG CCATGGGTAG AGAGCTCCTT GTTGCCTATC CGATATTTAG AGCCACAATG 
GAAGCGGCAG ATCAGTGCCT GGTTAAACTA GGTGCTATGT TTTCCTTGAT CGATGAGTTA 
TCCAAGGATA CTGGTACAAG TTTTATTTAT GAAGCATGTT TCAGTCAGCC CTCTTGTACA 
GCGATTCAGA TAGCTTTGAC AGAGCTTCTA AGTTCGTGGG GAATACAGCC TGTTTCCGTG 
GTTGGCCACT CTAGCGGTGA GATTGCTGCG GCGTATGCTG CCGGTATGCT TTCGCTCGAA 
GATTGCATCA CCATTGCCTA CACTCGGGGA ACCGTAGCAG ACCAAATATC GAAGGAATTT 
CCAGAAGTCA AAGGAGCCAT GTTGGCCGTT GGGGCGGGTG CATCGGATAT AGGCCCTCTT 
CTTGACACTC TTCAAGGAGG AAGTGCAGTA ATTGCCTGCG TCAATAGTCA AAAGAGTGTT 
ACGGTCTCTG GCGATGAAAC GGCAATAAGT GAATTGCAAA GCAAGGTTGA CGATCAAGGA 
ATTTTCAATA GAAAACTCCC AGTGAACGTA GCATATCACT CCTATCATAT GAGACTAGTT 
TCGGAAAAAT ATCGGTCGAT GATGGGAAAC ATCGTCCCCA AATCCGCAGC CATCAAATTC 
CACTCATCAG TGTTCGGTCA CCAAGCCACA TATTCCGATC TTACCGGTGA TTATTGGGTA 
GACAACTTCG TATCTCCTGT CGAGTTTGTA CGCGGCTTAC AAAGCCTCCT GTTGGAAAGT 
CGAGCTACCT CTGGCAAACA AATAAATATG CTTGTTGAGA TTGGACCACA CCCAGCACTG 
AAGGGCCCAA TCCGAGATAT TCTACAGCAT GATTCCCTGG GTGGAGAGAT AAAATATCTG 
CATAGTCTTA AACGCGAACA AGACGCCGTT GAGGCAATGC AGGCACTGGG GACGGAGTTA 
TTCATGAATG GGGCTAAACT CAACTTCAAC TCCATCAACT TTCCATCCGA CAAAGGAAAG 
AGACCTGTCT TGCTAACGAA TCTGCCGAGT TATCCTTGGA ACCACACTAC AAAATATTGG 
CAAACCTCCC GTCTGTCTCA AAATCACTGT CATAAGCCTT TTCCACGAAA CGATATTCTT 
GGCTCACTCT CTGTAGAAAG CGATGATATA GAACCCAGAT GGAGAAACGT TCTGCGGGCA 
GATGACCATC CATGGATTCG ACATCATAAA GTTCAAGGAA ACAATGTATA TCCTATGACT 
GGTTACCTTG CCATGGTGCT GGAAGCCGCA ACCCAGCATT CCATTATGAA AAAGGTAGAG 
TTCGATCACT TTGTATTTCG GGAAGTGTCT ATTGGGCGAG CCCTGATTAT TTCGGACACG 
TCTGCAGTCG AAACTATGAC TACTCTAAGA CCGTACGCTG AGGGCACTAG AATATCGTCG 
GATGCTTGGA GTGAATTTCG AGTATTTTCA TGGTCGGATA CTAAAGGATG GGATGAGCAT 
TGTCGAGGAC TTGTGAAGGT TTATAAAATC GGGGGCCCAA ACCCCATTGA TGGCTCTTAT 
CAGCACAGAG AGAACTCCCT TTATATCAAA GAGCAGGTAG CTGCATTTCA CTCCAATTGT 
GCATCTACCC CTGATGTCCA TGAAATGTAT GACGCTGTTG CTGCCAATGC TTTGGAGTAT 
GGCCCGTTAT TTCAAGCAGC ATCGAAAGTT TTCGTCGGTA ATCAACAAGC GATATTCGAC 
ATATCGGTGA CTGACACAAG AGGTTCTATG CCTCAAGAGA CTGAAACCGA CTTGATCATT 
CATCCTGCGG CTTTAGATGC GTGTGTGCAA ATTGTATGGC CTCTCTTGGG ATTTACTAAC 
CCGGGGCCGC ATTCCCTTTA CCTACCATCC TTCTTAAAAT GCCTGTCTGT TCCCTGCTCA 
ACGTCCATGC CAATTGGAGG CCAGTTTCGG GTCTATGGGA GTCGATTGAA CTCACTCTCT 
CATGGCGTTC CAATCAACCA CGATATATTT GTTACCTCCG CTGAAAATCC CACCGAAATT 
CTCTTAAAAA TGGAGGGGTT AACGTTAACA CCTGTTCATG ATGACATAGC GTCTGCGAAT 
GGTGAGCCCA AAGTGTTATG CTATAAGCTT TGCTTAGAGC CCTGCCTAGA GTTTTTGACC 
ACGGAAAATT TGAGATCATT TGATGGGGCT CATAACACGA ACCAGAACGA AAAGAGCCAT 
GTTCACATCC TCAATCAGGT TTCGTTTTAT TTCCTCGAGA AAGCGTTAAA ACAGCTTGAC 
AAAGAGGAAT ACCGTAGCCT CCGTCAGAAA CACGAGCCGT TCTATCAGTG GATACGAACT 
TTGAACTTAC AGACAGAGAA TGGAAGAATT AGACTTCATC ACTCAGATTG GTCTCAATTC 
AACGAGGAAA GGTGGTCATT CGTTATTAAC ATGGCTCGGT GTATGGGGAC AGTGGGGAAA 
GTGACATGTT CCATTGGTGA AAATTTTGTA CCGATTTTAA ATCGGGGAAT AGCCTCAATA 
TCTACCAGTC TTGGTGACGA TCTACTGGAA CAATTCTACG ATGAGCTTAG TCCCCTTAAC 
CGGAATTACG CCAACGCCGC CATTTGTATC GACAAGATGG CTTTCCAGTA CCCTGACATG 
GAAATTCTTG AGATCGGAGC TGGCACAGGG GAAGGATCTT TACGCATACT GCGAATTTTG 
GGGGGTGGTG ACACTGGAAA AATGCGGAGA TTCGCCAAAT ACACGTACAC TGCTAAAAGC 
GCCCATACAC TCGTTAAAGC TAAGCCTAAG TTTCAAGCCT GGGGTAGGTT CGTGTCATGT 
CGAAACTTGA ATATTTCGCA AGATCCTTCT GGCCAAGGTT ATTTACCAAA ATCGTACGAT 
CTAATAATCG CTTGTCATTT ATCGGATTCG ACAGAATGTT TACATGAAGC CATGGCCAAT 
GTGAATGGAC TTCTGAAACC TGGAGGGAAA CTGGTTTTGC TCGATTGGAC GGCCAGACAT 
TTATATCAGT TCGTTCCCAA ATTGCTTTCA GGCTCTTGGT CAACCGAAAG ATCAGGTCGT 
GCGGGTAGTT CTATTCGGAA TAAAGACGAG TGGGACAAGC TGTTCCGAGA AACAAAGTTT 
TCTGGATTAG ACCTATCTCT GGACGACTAT CCAGAGACCC TTGAGCAGTC CAGTAGTCTG 
ATGCTATCAA CCGTCCAAGC ATCCGATGAA TTATCCGGCA AGGAAATAGC TCTAGTTTAC 
CACGGGGGAG TCAATGACTC TCTCGCACCT GGCCTTATCC GCGAGTTGGA AAATATAACT 
GGCATCCGCC CCTCCGTTGA AGAACTCTGC CAAGTTAATG CAAAAGGTAG ACTATGCATT 
TTTTTCGGCG AACTATATCA GCCGCTTTTA TCCAGCATGA CACCGGATCT TCTTTCAGCA 
ATTCAACTTC TAGTATCTAG TGCCAGTGGT ATTCTCTGGG TCATTCGTCA AGACCACCTA 
GTCTCGGGCT CACCTGAATC CAGCATGGCG ATCGGGCTAG CCAGAACATT ACGCTCAGAA 
ACGGCGATAC AACTTGCGAC TCTAGAGCTG GAAGGCAATC CCCACTATGG CGAAGCTGAC 
CAAATAATGA AGGTCTTCAG AGCTGTATTT GGTACTGAAC CTAGTCTCAG GCAAGGAGAT 
ATGGAATATG TGGTTAAAAA TAGACTTATT TGTGTTTCGC GCGTTTTTGC AGATCCAGAA 
ACGAACAAAT ATGTTCACGA AGAGTCACAC AGAATCCCCC CTGAACTACA GCCTTTCTAT 
CAAAACAACA GGCGCCTCAA ACTAGCGATG GGACATCATG GCACTCTGGA CAGTTCTTAT 
TTTACGGATG AGCTCTCAAC AACTCAGGTC CCAGCCAATT GGATAGAAAT CGAAACTCGA 
TTTATAGGAC TAAATTTCAA AGATATTATG ATTGCCATGG GTCAACTCCA AAACGGTCAT 
TTAGGGAATG AATGTAGCGG AGTCGTTACC GCGATTGGGG ATAAAGTGAC GGACTTCAAA 
GTTGGCGACC GGATTTGTGC CGTCTCTGAG GGAGCTTTTG CCAATTACGC TCGCTTTCCG 
GCATCAAGCG CCTGGAAGAT TCCAGATAAG ATGAGTTTTG ATGTAGCAGC ATCCATTCCA 
ATTGTATTTT GCACAGCGTA TTACTCTTTG TTTGATGTTG GTCGTCTGCA ACGGGGGGAG 
CGGATTCTCC TTCATGCCGC CGGAGGAGGG GTAGGCCAAG CGGCCATTAT CCTCGCTCAA 
TCGATTGGTG CGGAGATTTA TGCAACTGTT GGTAGTGCTG ATAAGAAGAA GTTTCTAATG 
GAAACCTACA ACATCGACGA AACAAGAATC TTTTTCAGTC GGGATATTTC TTTCGCTGAC 
AGAATCCGCA AAGCCACGGA TGGAGCAGGC GTTGATGTTG TCTTGAATTC TCTTGCTGGT 
GAAATGCTAC AGGCAACTTG GGAATGCATT GCTCCGTTTG GGCGATTCAT AGAAATTGGT 
AAAAAGGACA TTGTACGGAA TTCGAGACTT GAAATGATCC ACTTCGACCG GAACGTAAGT 
TTTGCATCTG TTGATTTGAC ACTCTTGGCC AACAAACGCC CGCAGTTGAT GAAAAAAGTT 
CTAGCTGGAG TCTTTGGGAT GTTTGAAAAT GGCAGTGCTA GGAGTATATC GCCAATTACG 
CGCTTTTCAA TATCGGATTT CGAGAAGGCG TGTCATAATT TACAGACTGG CCGAAGTATC 
GGAAAGATTG TCATCGAAGC GGGGAAGAAT GCTTGCGTCA AGGTTTATCC AAGAAAGAAG 
CAAGGAGATA TTCTCTTTGC GGAAGCCTCC TACATAGTCG TGGGTGGTAC GGGAGGGCTC 
GGACGAAATA TTACGTCATG GTTAGCTGAG AAAGGGGCGC GACATATCAT TGTCGTCTCA 
CGAAGCGGTG CCTCGGATGA AAGAGTTGGG GAGATGATTA AGGATCTCGC TGCAGACGGA 
GCAACTGTCG TTGTATGCCA ATGCGATATC AGCAAAAAAG AGGAAGTCGA AACCAAACTG 
GCAACAATAC TACCGCAAGC GCCACCTGTG CGCGGGGTAA TATATTGCGC AATGGTTCTC 
CGGGACACGC TGTTCGAACG GATGACCTTT GATGACTACG ACGGGGTTGT GAAACCAAAG 
GTTGCAGGAG TGTGGAATCT ACACCATGCG CTCGTAGGAT TAAGCTGTGA TCTTGATTTC 
TTCATCACTC TCTCTTCAAT TGCTAGTCTT GTCGGTAACA GGGGACAAGC TGCATATGCC 
GCTGGTGGCA CTTTCATGAC AGCTTTCGCA CAATATGGAA ACGCGCGTGG TTTACCTTGC 
ACTACTATCG ATCTGGCTCC CGTTCAAGGA ATCGGGTATC TCGCGGAAAA TCAGCAGAGG 
AAGAACGAAG TCGAAAACTC GCTAGTCGTG GATTGGGTTA ATGAAACCCA GCTTCGAGGA 
CTTCTTGCCG CCGCCATCAG AGGCGATATG GCCAATACCT GTCACCACCA CTGCATCACC 
GGCCTAGGAA TATTGAAATC CATCTCCGGT AAAGAGACAC CGTGGTGGGC CTTGGATCCA 
AGGTTTTCAC ATCTCGTTCA GTCGTGCGCC TCTGATGAAG GCCAAACATG CGACCCCGCA 
TCTTCTCTAG CCACAGCATC TCCCGGTCTT GCGGTGAAAA ATACAGCTGA TCGCGCAGAC 
GCAGAAAGAA TAGTTACGGA TGCTCTTGTA CAAAAAGTAT CCACCATAAT GATGCGGTCC 
GTCGACGAAA TTGACCCTTC AAAACCTCTG GGCACATACG GGCTGGACTC CCTGATCACC 
ATTGAGGTGC GCAATTGGAT CTCTCGCGAG CTCCAAGCGA GCTTGCAAAT CATGGAGATT 
CTCGTGACCA AGTCTGTGGT AGAGCTGGCG GGGCTGATAT TGAAGAAATC AAAGATCGTG 
AGCGTGGAGG CGAAATCGCG ATGGGGGCTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis.
PLoS genetics7(10)e1002345(2011 Oct)
Desjardins CA,Champion MD,Holder JW,Muszewska A,Goldberg J,Bailão AM,Brigido MM,Ferreira ME,Garcia AM,Grynberg M,Gujja S,Heiman DI,Henn MR,Kodira CD,León-Narváez H,Longo LV,Ma LJ,Malavazi I,Matsuo AL,Morais FV,Pereira M,Rodríguez-Brito S,Sakthikumar S,Salem-Izacc SM,Sykes SM,Teixeira MM,Vallejo MC,Walter ME,Yandava C,Young S,Zeng Q,Zucker J,Felipe MS,Goldman GH,Haas BJ,McEwen JG,Nino-Vega G,Puccia R,San-Blas G,Soares CM,Birren BW,Cuomo CA

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PAAG_02977 cDNA ORF clone, Paracoccidioides lutzii Pb01

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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