Dpse\GA23740 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA23740
Entrez Gene ID	6900518
Full Name	GA23740 gene product from transcript GA23740-RA
Synonyms	GA23740,dpse_GLEANR_13011
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015187386.1	XP_015042872.1	uncharacterized protein, isoform B
XM_002134869.2	XP_002134905.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA23740 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA23740 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe12584	XM_002134869.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA23740), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe26238	XM_015187386.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA23740), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA23740 ( XM_002134869.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002134905.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA23740), mRNA.

Price & Availability↑

CloneID	ODe12584
Clone ID Related Accession (Same CDS sequence)	XM_002134869.2
Accession Version	XM_002134869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_002134869.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAACCG AGACTACCAA AATGATCTAT ACCCCAGCAG ACATCAAAAT GGAAGTTGAA 
ATCGGTGAAC CACCACAGCC GCCAGTGAAA TGTTCCAACT TCTTCAGCAA TCACTGGAAG 
GGCCTTGTGG TCTTTCTGGT TCCTCTGTTA TGCCTGCCCA TAATGCTGCT GAACAATACA 
CCCGAATATC GCTGCATGTA TCTGCTATTG GTGATGGCCG TGTTCTGGGT GACAGAAGCC 
CTGCCCCTCT ATGTGACCTC AATGATACCC ATTGTGGCGT TCCCCGTGAT GGGAATTATG 
AGCTCCGATC AGACCTGTCG TTTGTACTTC AAAGACACGC TGGTCATGTT CATGGGTGGC 
ATTATGGTGG CCCTGGCCGT GGAATACTGT AATCTGCACA AGCGCCTCGC CTTGAGGGTA 
ATCCAGATCG TGGGCTGCAG TCCCCGGAGA TTACACTTTG GCCTGATTAT GGTTACCATG 
TTTCTGTCCA TGTGGATCTC GAACGCCGCC TGCACGGCTA TGATGTGTCC GATTATTCAG 
GCTGTGCTGG AGGAGCTGCA GGCCCAGGGC GTCTGCAAGA TCAACCACGA GCCCCAATAT 
CAAATTGTCG GGGGCAACAA GAAGAACAAC GAGGATGAGC CACCATATCC CACCAAGATC 
ACTCTTTGCT ACTATTTGGG CATTGCCTAC GCCTCATCGC TGGGCGGCTG TGGAACCATC 
ATTGGAACGG CCACCAATCT CACCTTCAAG GGCATCTACG AGTCGCGTTT CCCCACCTCC 
ACCGAACAGA TGGATTTCCC CACATTCATG TTCTACTCGG TGCCAGCGAT GTTGATCTAC 
ACATTCCTGA CATTCCTCTT CCTGCAGTGG CACTTCATGG GCCTGTTCCG TCCCAAGAGC 
AAGGAGGCTC AGGAAGTGCA GTTGGGCAAG GATGGCGCCG ATGTGGCCAA GAAAGTGATC 
GATCAGCGTT ACAAGGAGCT GGGCCCCATG AGCATCCACG AGATTCAAGT GATGATTCTG 
TTTATATTCA TGGTCATCAT GTACTTCACC CGCAAACCTG GCATCTTCCT GGGCTGGGCC 
GATTGGCTGA ACACCAAGGA CATTCGCAAC TCTATGCCAA CTATTTTCGT CGTCATCATG 
TGCTTCATTC TGCCAGCGAA CTATGCTTTC CTGCGCTACT GCTCGAGACG TGGCGGTCCA 
GTGCCCACGG GCCCGACCCC CTCTCTGATT ACCTGGAAGT TCATCCAGAC CAAGGTGCCA 
TGGGGCTTGG TCTTCCTTTT GGGCGGTGGC TTTGCCCTGG CCGAGGGCAG CAAAGTGAGC 
GGCATGGCCA AGCTGATTGG CAACGCCCTG ATTGGCCTGA AGGTCTTGCC AAATGCTGTA 
CTCCTGCTGG TGGTCATCCT GGTTGCTGTT GTGCTCACGG CCTTCAGTTC CAATGTGGCT 
ATTGCCAACA TTATTATTCC CGTACTGGCG GAGATGTCGC TGGCCATTGA GATTCATCCG 
CTGTATCTGA TTCTGCCCGC TGGTCTGGCC TGCAGCATGG CCTTCCACCT GCCAGTGAGC 
ACGCCACCCA ATGCTCTGGT TGCCGGCTAT GCCAACATTA GAACAAAGGA CATGGCCATT 
GCTGGAATCG GCCCCACCAT CATCACCATC ATTATCCTGT TTATCTTCTG TCAAACCTGG 
GGCCTGGTTG TCTACCCGAA CCTCAACTCG TTCCCCGAGT GGGCGCGTCT CGCGGCCGAA 
GCAGCTGTCA ACAAGACGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002134905.1
CDS	177..1940
Translation	MATETTKMIYTPADIKMEVEIGEPPQPPVKCSNFFSNHWKGLVVFLVPLLCLPIMLLNNTPEYRCMYLLLVMAVFWVTEALPLYVTSMIPIVAFPVMGIMSSDQTCRLYFKDTLVMFMGGIMVALAVEYCNLHKRLALRVIQIVGCSPRRLHFGLIMVTMFLSMWISNAACTAMMCPIIQAVLEELQAQGVCKINHEPQYQIVGGNKKNNEDEPPYPTKITLCYYLGIAYASSLGGCGTIIGTATNLTFKGIYESRFPTSTEQMDFPTFMFYSVPAMLIYTFLTFLFLQWHFMGLFRPKSKEAQEVQLGKDGADVAKKVIDQRYKELGPMSIHEIQVMILFIFMVIMYFTRKPGIFLGWADWLNTKDIRNSMPTIFVVIMCFILPANYAFLRYCSRRGGPVPTGPTPSLITWKFIQTKVPWGLVFLLGGGFALAEGSKVSGMAKLIGNALIGLKVLPNAVLLLVVILVAVVLTAFSSNVAIANIIIPVLAEMSLAIEIHPLYLILPAGLACSMAFHLPVSTPPNALVAGYANIRTKDMAIAGIGPTIITIIILFIFCQTWGLVVYPNLNSFPEWARLAAEAAVNKTH

Target ORF information:

RefSeq Version	XM_002134869.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA23740), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002134869.2

ORF Insert Sequence:

ATGGCAACCG AGACTACCAA AATGATCTAT ACCCCAGCAG ACATCAAAAT GGAAGTTGAA 
ATCGGTGAAC CACCACAGCC GCCAGTGAAA TGTTCCAACT TCTTCAGCAA TCACTGGAAG 
GGCCTTGTGG TCTTTCTGGT TCCTCTGTTA TGCCTGCCCA TAATGCTGCT GAACAATACA 
CCCGAATATC GCTGCATGTA TCTGCTATTG GTGATGGCCG TGTTCTGGGT GACAGAAGCC 
CTGCCCCTCT ATGTGACCTC AATGATACCC ATTGTGGCGT TCCCCGTGAT GGGAATTATG 
AGCTCCGATC AGACCTGTCG TTTGTACTTC AAAGACACGC TGGTCATGTT CATGGGTGGC 
ATTATGGTGG CCCTGGCCGT GGAATACTGT AATCTGCACA AGCGCCTCGC CTTGAGGGTA 
ATCCAGATCG TGGGCTGCAG TCCCCGGAGA TTACACTTTG GCCTGATTAT GGTTACCATG 
TTTCTGTCCA TGTGGATCTC GAACGCCGCC TGCACGGCTA TGATGTGTCC GATTATTCAG 
GCTGTGCTGG AGGAGCTGCA GGCCCAGGGC GTCTGCAAGA TCAACCACGA GCCCCAATAT 
CAAATTGTCG GGGGCAACAA GAAGAACAAC GAGGATGAGC CACCATATCC CACCAAGATC 
ACTCTTTGCT ACTATTTGGG CATTGCCTAC GCCTCATCGC TGGGCGGCTG TGGAACCATC 
ATTGGAACGG CCACCAATCT CACCTTCAAG GGCATCTACG AGTCGCGTTT CCCCACCTCC 
ACCGAACAGA TGGATTTCCC CACATTCATG TTCTACTCGG TGCCAGCGAT GTTGATCTAC 
ACATTCCTGA CATTCCTCTT CCTGCAGTGG CACTTCATGG GCCTGTTCCG TCCCAAGAGC 
AAGGAGGCTC AGGAAGTGCA GTTGGGCAAG GATGGCGCCG ATGTGGCCAA GAAAGTGATC 
GATCAGCGTT ACAAGGAGCT GGGCCCCATG AGCATCCACG AGATTCAAGT GATGATTCTG 
TTTATATTCA TGGTCATCAT GTACTTCACC CGCAAACCTG GCATCTTCCT GGGCTGGGCC 
GATTGGCTGA ACACCAAGGA CATTCGCAAC TCTATGCCAA CTATTTTCGT CGTCATCATG 
TGCTTCATTC TGCCAGCGAA CTATGCTTTC CTGCGCTACT GCTCGAGACG TGGCGGTCCA 
GTGCCCACGG GCCCGACCCC CTCTCTGATT ACCTGGAAGT TCATCCAGAC CAAGGTGCCA 
TGGGGCTTGG TCTTCCTTTT GGGCGGTGGC TTTGCCCTGG CCGAGGGCAG CAAAGTGAGC 
GGCATGGCCA AGCTGATTGG CAACGCCCTG ATTGGCCTGA AGGTCTTGCC AAATGCTGTA 
CTCCTGCTGG TGGTCATCCT GGTTGCTGTT GTGCTCACGG CCTTCAGTTC CAATGTGGCT 
ATTGCCAACA TTATTATTCC CGTACTGGCG GAGATGTCGC TGGCCATTGA GATTCATCCG 
CTGTATCTGA TTCTGCCCGC TGGTCTGGCC TGCAGCATGG CCTTCCACCT GCCAGTGAGC 
ACGCCACCCA ATGCTCTGGT TGCCGGCTAT GCCAACATTA GAACAAAGGA CATGGCCATT 
GCTGGAATCG GCCCCACCAT CATCACCATC ATTATCCTGT TTATCTTCTG TCAAACCTGG 
GGCCTGGTTG TCTACCCGAA CCTCAACTCG TTCCCCGAGT GGGCGCGTCT CGCGGCCGAA 
GCAGCTGTCA ACAAGACGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA23740 ( XM_015187386.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015042872.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA23740), mRNA.

Price & Availability↑

CloneID	ODe26238
Clone ID Related Accession (Same CDS sequence)	XM_015187386.1
Accession Version	XM_015187386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGTTG AAATCGGTGA ACCACCACAG CCGCCAGTGA AATGTTCCAA CTTCTTCAGC 
AATCACTGGA AGGGCCTTGT GGTCTTTCTG GTTCCTCTGT TATGCCTGCC CATAATGCTG 
CTGAACAATA CACCCGAATA TCGCTGCATG TATCTGCTAT TGGTGATGGC CGTGTTCTGG 
GTGACAGAAG CCCTGCCCCT CTATGTGACC TCAATGATAC CCATTGTGGC GTTCCCCGTG 
ATGGGAATTA TGAGCTCCGA TCAGACCTGT CGTTTGTACT TCAAAGACAC GCTGGTCATG 
TTCATGGGTG GCATTATGGT GGCCCTGGCC GTGGAATACT GTAATCTGCA CAAGCGCCTC 
GCCTTGAGGG TAATCCAGAT CGTGGGCTGC AGTCCCCGGA GATTACACTT TGGCCTGATT 
ATGGTTACCA TGTTTCTGTC CATGTGGATC TCGAACGCCG CCTGCACGGC TATGATGTGT 
CCGATTATTC AGGCTGTGCT GGAGGAGCTG CAGGCCCAGG GCGTCTGCAA GATCAACCAC 
GAGCCCCAAT ATCAAATTGT CGGGGGCAAC AAGAAGAACA ACGAGGATGA GCCACCATAT 
CCCACCAAGA TCACTCTTTG CTACTATTTG GGCATTGCCT ACGCCTCATC GCTGGGCGGC 
TGTGGAACCA TCATTGGAAC GGCCACCAAT CTCACCTTCA AGGGCATCTA CGAGTCGCGT 
TTCCCCACCT CCACCGAACA GATGGATTTC CCCACATTCA TGTTCTACTC GGTGCCAGCG 
ATGTTGATCT ACACATTCCT GACATTCCTC TTCCTGCAGT GGCACTTCAT GGGCCTGTTC 
CGTCCCAAGA GCAAGGAGGC TCAGGAAGTG CAGTTGGGCA AGGATGGCGC CGATGTGGCC 
AAGAAAGTGA TCGATCAGCG TTACAAGGAG CTGGGCCCCA TGAGCATCCA CGAGATTCAA 
GTGATGATTC TGTTTATATT CATGGTCATC ATGTACTTCA CCCGCAAACC TGGCATCTTC 
CTGGGCTGGG CCGATTGGCT GAACACCAAG GACATTCGCA ACTCTATGCC AACTATTTTC 
GTCGTCATCA TGTGCTTCAT TCTGCCAGCG AACTATGCTT TCCTGCGCTA CTGCTCGAGA 
CGTGGCGGTC CAGTGCCCAC GGGCCCGACC CCCTCTCTGA TTACCTGGAA GTTCATCCAG 
ACCAAGGTGC CATGGGGCTT GGTCTTCCTT TTGGGCGGTG GCTTTGCCCT GGCCGAGGGC 
AGCAAAGTGA GCGGCATGGC CAAGCTGATT GGCAACGCCC TGATTGGCCT GAAGGTCTTG 
CCAAATGCTG TACTCCTGCT GGTGGTCATC CTGGTTGCTG TTGTGCTCAC GGCCTTCAGT 
TCCAATGTGG CTATTGCCAA CATTATTATT CCCGTACTGG CGGAGATGTC GCTGGCCATT 
GAGATTCATC CGCTGTATCT GATTCTGCCC GCTGGTCTGG CCTGCAGCAT GGCCTTCCAC 
CTGCCAGTGA GCACGCCACC CAATGCTCTG GTTGCCGGCT ATGCCAACAT TAGAACAAAG 
GACATGGCCA TTGCTGGAAT CGGCCCCACC ATCATCACCA TCATTATCCT GTTTATCTTC 
TGTCAAACCT GGGGCCTGGT TGTCTACCCG AACCTCAACT CGTTCCCCGA GTGGGCGCGT 
CTCGCGGCCG AAGCAGCTGT CAACAAGACG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015042872.1
CDS	152..1867
Translation	MEVEIGEPPQPPVKCSNFFSNHWKGLVVFLVPLLCLPIMLLNNTPEYRCMYLLLVMAVFWVTEALPLYVTSMIPIVAFPVMGIMSSDQTCRLYFKDTLVMFMGGIMVALAVEYCNLHKRLALRVIQIVGCSPRRLHFGLIMVTMFLSMWISNAACTAMMCPIIQAVLEELQAQGVCKINHEPQYQIVGGNKKNNEDEPPYPTKITLCYYLGIAYASSLGGCGTIIGTATNLTFKGIYESRFPTSTEQMDFPTFMFYSVPAMLIYTFLTFLFLQWHFMGLFRPKSKEAQEVQLGKDGADVAKKVIDQRYKELGPMSIHEIQVMILFIFMVIMYFTRKPGIFLGWADWLNTKDIRNSMPTIFVVIMCFILPANYAFLRYCSRRGGPVPTGPTPSLITWKFIQTKVPWGLVFLLGGGFALAEGSKVSGMAKLIGNALIGLKVLPNAVLLLVVILVAVVLTAFSSNVAIANIIIPVLAEMSLAIEIHPLYLILPAGLACSMAFHLPVSTPPNALVAGYANIRTKDMAIAGIGPTIITIIILFIFCQTWGLVVYPNLNSFPEWARLAAEAAVNKTH

Target ORF information:

RefSeq Version	XM_015187386.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA23740), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187386.1

ORF Insert Sequence:

ATGGAAGTTG AAATCGGTGA ACCACCACAG CCGCCAGTGA AATGTTCCAA CTTCTTCAGC 
AATCACTGGA AGGGCCTTGT GGTCTTTCTG GTTCCTCTGT TATGCCTGCC CATAATGCTG 
CTGAACAATA CACCCGAATA TCGCTGCATG TATCTGCTAT TGGTGATGGC CGTGTTCTGG 
GTGACAGAAG CCCTGCCCCT CTATGTGACC TCAATGATAC CCATTGTGGC GTTCCCCGTG 
ATGGGAATTA TGAGCTCCGA TCAGACCTGT CGTTTGTACT TCAAAGACAC GCTGGTCATG 
TTCATGGGTG GCATTATGGT GGCCCTGGCC GTGGAATACT GTAATCTGCA CAAGCGCCTC 
GCCTTGAGGG TAATCCAGAT CGTGGGCTGC AGTCCCCGGA GATTACACTT TGGCCTGATT 
ATGGTTACCA TGTTTCTGTC CATGTGGATC TCGAACGCCG CCTGCACGGC TATGATGTGT 
CCGATTATTC AGGCTGTGCT GGAGGAGCTG CAGGCCCAGG GCGTCTGCAA GATCAACCAC 
GAGCCCCAAT ATCAAATTGT CGGGGGCAAC AAGAAGAACA ACGAGGATGA GCCACCATAT 
CCCACCAAGA TCACTCTTTG CTACTATTTG GGCATTGCCT ACGCCTCATC GCTGGGCGGC 
TGTGGAACCA TCATTGGAAC GGCCACCAAT CTCACCTTCA AGGGCATCTA CGAGTCGCGT 
TTCCCCACCT CCACCGAACA GATGGATTTC CCCACATTCA TGTTCTACTC GGTGCCAGCG 
ATGTTGATCT ACACATTCCT GACATTCCTC TTCCTGCAGT GGCACTTCAT GGGCCTGTTC 
CGTCCCAAGA GCAAGGAGGC TCAGGAAGTG CAGTTGGGCA AGGATGGCGC CGATGTGGCC 
AAGAAAGTGA TCGATCAGCG TTACAAGGAG CTGGGCCCCA TGAGCATCCA CGAGATTCAA 
GTGATGATTC TGTTTATATT CATGGTCATC ATGTACTTCA CCCGCAAACC TGGCATCTTC 
CTGGGCTGGG CCGATTGGCT GAACACCAAG GACATTCGCA ACTCTATGCC AACTATTTTC 
GTCGTCATCA TGTGCTTCAT TCTGCCAGCG AACTATGCTT TCCTGCGCTA CTGCTCGAGA 
CGTGGCGGTC CAGTGCCCAC GGGCCCGACC CCCTCTCTGA TTACCTGGAA GTTCATCCAG 
ACCAAGGTGC CATGGGGCTT GGTCTTCCTT TTGGGCGGTG GCTTTGCCCT GGCCGAGGGC 
AGCAAAGTGA GCGGCATGGC CAAGCTGATT GGCAACGCCC TGATTGGCCT GAAGGTCTTG 
CCAAATGCTG TACTCCTGCT GGTGGTCATC CTGGTTGCTG TTGTGCTCAC GGCCTTCAGT 
TCCAATGTGG CTATTGCCAA CATTATTATT CCCGTACTGG CGGAGATGTC GCTGGCCATT 
GAGATTCATC CGCTGTATCT GATTCTGCCC GCTGGTCTGG CCTGCAGCAT GGCCTTCCAC 
CTGCCAGTGA GCACGCCACC CAATGCTCTG GTTGCCGGCT ATGCCAACAT TAGAACAAAG 
GACATGGCCA TTGCTGGAAT CGGCCCCACC ATCATCACCA TCATTATCCT GTTTATCTTC 
TGTCAAACCT GGGGCCTGGT TGTCTACCCG AACCTCAACT CGTTCCCCGA GTGGGCGCGT 
CTCGCGGCCG AAGCAGCTGT CAACAAGACG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA23740 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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