Dpse\GA21159 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA21159
Entrez Gene ID	4817010
Full Name	GA21159 gene product from transcript GA21159-RA
Synonyms	GA21159,dpse_GLEANR_2379
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015180406.1	XP_015035892.1	uncharacterized protein, isoform B
XM_001356484.3	XP_001356520.2	uncharacterized protein, isoform A
XM_015180407.1	XP_015035893.1	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA21159 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA21159 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe15528	XM_001356484.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA21159), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe17804	XM_015180406.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA21159), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe17805	XM_015180407.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA21159), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA21159 ( XM_001356484.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001356520.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA21159), mRNA.

Price & Availability↑

CloneID	ODe15528
Clone ID Related Accession (Same CDS sequence)	XM_001356484.3
Accession Version	XM_001356484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589964). On Dec 22, 2015 this sequence version replaced XM_001356484.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAAA GATCCAAAGG TGACGTGTCT CGCGTAAACA ACAATCCAAA TGCGCCGCGT 
GTCGTTAAAA ATCCATTACT TAGCGCCTCG GTAACCGGAC TCAGCTACGA AGACTTTCCC 
AACAAGCCGC AACTGTTGCC CGCCGCCCCG CCCGTGGTGC ATGCCCCGCC GCCAGCATCC 
ACACCCGTCC TGATCGCTGG CATTCTGAAT CGCGAGCCAA AAACTTTGGT GACCGTGGGA 
CAGCCATCGA GCATCGATGA CAGCGAAGCC CTCGACGAGA TCAACTTGAA TACGAGCAGC 
GAGGACTCGA GTGGAGTCAG TGGTGGATCG TCGACTGCGT ATCTGCGTGG CACAGGCGAT 
GGTAATGCCA ATCCCTTGCT AGAACCACCA CCGGGCGCTG GAAACGATTC GTTGTTGAGC 
CAGGCGGCCT CATCCTTCAC AGCACTGCCC TCGGTGGCCT CCAATGTGTT TTCCACCTTC 
TCCAAGAGAA TCTACGCGGG CACAAAGGAA TCGGAAGAGC CTAAATTGGA CATCCAGCCC 
ACGTACATAC AGCAGGCGGG ACACTACGCC CCACCGCCAG TAGCTGCAGC ACCACCACCA 
CCATTCTACG CGGCACCACC GGCGGCAGCG AATGAGATTG TTGCTGCCCC AGAACCACCC 
AAGTTCTATG CCCCCACGGA GGTGCCAGCC CCGAACGTAG GCATCGCCAG TGCACCACCA 
CCCACCGCAT CGGGAAATCC CAACACCTAT CGATTCACCG CCAGAAAGAA GCTATACGCA 
CCGATTCCTG GCTTCACCGA GCAGTCGGCA CAGGGTCCGC AGATTCCACA GGCCCCACAG 
GCAGGTCTGC CCTTCCAGAC GCCACCATAT CCAGCCCAAC AGGCAGCTCC ATTTGAGATA 
TCATCAGCAT CTCCTTTTGA GATTCAAGCA GCTCCCCCAG CAGCAGCAGC GACTGCCACA 
GAGGAGCGCA AATCCGGCGG AGGACTCTTT TCTCTGAGCA ATCTTGTGCC CACTGGCGTG 
CTGCAAAACA TCTCCGGACT TGTACAGTCC GCCACAGGCA GGAGCAGCGA GCAGTCCTCT 
AGTAATCCTC CACCATCTGG CGGGGGCTAC TTTGACATCA ACACAGTCGC TCCAGCAAAT 
CCGAGCAACT ATTTCGGTGG CCCACCAGCG GCGGCACCGC CAGCGGGAAG CTACTTTGTG 
CCTGGCGCAG TTCCTGAGGC AGCGACAGCA ATTCCTCAAC CACCAGTAGG TGGGTTCTTT 
CCACAGCCAG CCAATCCGAT ACCGACAGCT GCTCCCACTT CAGTCGCGGC TCCTCCCACA 
GTTGGAGGAT TCTTTGGGGC AATACCACCT GCTAATCCGC CAGCAACTGA TATCAATCCC 
ATCTCGGGAG CACCACCAGT TGGAGGATTC TTTACACCAG GTGTAGCAGC AGTTCCTCCC 
ACAGTTGGAG GATTTTATAC GCCCGTTTCG GCACCGATTG AGATCAATCC TATTGCTGAA 
GCACCACCAC CGGCAGAAGC AGCTATTCCC CCATCATCTG CACTTGCAGG AGGATTTACT 
GCAACCCCAG GAGCAGTGCC ATCTGTGACT ACATCCACGA CAGCAGCTCC TCCGCCAATT 
GGAGGGTTCT TTGATCCTAA TCCAGCAGCA TCTGGTATTC CTCCCACTTC CACAGTTGGA 
GGATTCTTTA CGCCAGCAGC AGTTCCACCT GTGGCCACAG CAGCACCTGT TCCACCTGTT 
GCCACAGTAA CAGCAGCAGC TCCTCCACCA GTAGGAGGAT TCTTTGCACC CGCAGGAGCA 
GGGCCACCAT CCAACGCAAC TCCACTGGCA GGACCACCTC TGGGGACAGC ACCTCCATTG 
GCAGGACCAC CTTTAGGATC AGCAGCAACT GTCGCTGGAC CACCACCTGC CGCTGGACAG 
GCATCGTATC GCCTCCAGAA GGGCACACGT CTCTACAAGA GTCCATTGAC GGCACAGGAA 
ACTGCCACAG CGTCTGCTTT CGGCACTCCC TTTGCCACAA CAGCAGCTGT CCCAGCTGCC 
GTGTTCAATC CATTTGGAAC TGGAGCTTCA GGAGTATTTA ATCCATTAGC TGGCAACCCA 
CCAGTTGGTC AGCAGGAGAA CGAAGATAAC TCAGCCGTTG CACTATTTGC ACCTCCTTCT 
GCACCAGTGG CAGGACAGAC CTTAGATCAA GGACCGGCTC CTCCAGCTGC CTTTGCATCG 
ACAAATCAGG GAAGTCCCTT CCCCGCTCCA CAGCCAGGAC CCCAGGCAAC GCCATTCTTC 
ATTCCACAAA CGGGGGACAG ACCTCCAGTA CCCACCAGCC AGGAAACTGC ACCATCCAGC 
CAAGGAATAT CCTTCTTTGA AGCACCTCCG ACATCGGACA TTGCTCTCTT CACACCGCCA 
CCCGTCCAGG AAACAGCAGC AGCACCAGTC GAAACACAAG GAATATCTTT CTTTGCACCG 
CAGCCAGATC TCGCACCGAA AGCAGCCCAA GAGATTCCAC TGTTTGTCCC GGCAGCAGTT 
GAGGCACCTC CAGAAGGACA TCCCTTGTAT CCACCGACAT CTACAGCAGT CGCAGAGGAC 
ATTCCTGCCG CTGAGAGCCC ATTATTTGTG CCAGTGCCAA CATCGGCAGC AGATCAGAGT 
CAGGGAGTAC CGCTCTATCC ACCAGAAACA GCCACATCTA ACGCTTCATC TAGCGTTCTC 
TTTGCTGCAT CGGAGCATCA ACCGAGTGCA CCATTATTCG AAGCTGTTCA ACCACCAACC 
ACTGAGGCAG TCGATGCTTC AGCAAGCGAT TTCTTTGGAT CTGCCAATGC TGAAGCTGTC 
CCTGCATCTG CAAATACCGA AACGGCTTAC AGCCCATTCG GACAGACGGT GACCACACCC 
TCGCAAGTGC TGCAGACACC ACCGGCACAA GATTTACTGC CACCGCCTAT TGGATTTGTT 
GCGCCACAAC AACCAGAGGC AGAGCCAGAG CCAGAGGTGG CCGCCGCCGC TCCTCCAGCA 
GAATTCGGAG GAGTTACCGA AGCACTAGCC CTAGCCCACC CACCACTTGC ACCATCGCCG 
CTTCAGAGCT TCTTTTCGCA GCCAGCGACT GCTGCCCCAG AGGATTTCAA CTTTTTTGCC 
GCACCAGGCA GCAGCCTGTT TCCAGAACTG CCACAGCAGC GGGGCATAGC GCCGCCCCCT 
TTGGCACAGG AGCCACCGCC GGCAGCAGCA GAATCTGGTA TTGCACCAGG ACTGGGCTTT 
GATCAATTTC TAAGCCAATC CCAGCAAGCG CAAAGCCACA ACTACATTGG AAATTCAAAT 
GAGAATTCCT ATCAACAAAA TTCAAGTGTC AATACATTCT CCGGCTATTT TGGTGGCCCC 
ACTGTGACAG AAGCCCCAGC CCCACCCCCA GCCACAACCA CAGCAGCAGC AGTGCCACTT 
GCTGCTCCAG TTAGTAACTG TGACCCCTCC CATTTCTTTG ATCAAGTGCC ACAGCCACAG 
GCCCCGACAA ACGCTAACGA GGATCAACGC ATACAGAACT TCTTCAACAA CCCGCCACTG 
CAGGATCAGC CAGCGGCACC GGGCGAACTC AAATACGATA TAGTGCACAG CGGTCTGCCC 
GTCAAACAGC TACAGGCACG CTCCCAGACA CCCATCTCCA ATTTGGTGGA GCCCCCATCC 
TCGTCTGCCT GCTCAGAGTT TTCGACACTG GCACCAGCAG CACCCACAGC TCCAGCAACA 
GCTCCGGCAA CCAATCCAAC AGTCGAGCGT CAAACGGGCG AGGATCTACT GCAGTTGCAT 
CAAGCGGAAT TGCCGGAGGA GATCTTGAAA GAGCTCAGAA TGGCCGCAAA TGCAACGACA 
AGCGACAAGG CAGGCACACC CCCTGTTTCT ATAGTTTATA CGCCTGCTGT GGTCCATTGG 
TTCTACAAAC GTAGAGTGGA CGCGAAACAT ATTTGGACAC CATTCAGTCA CTATGATTCA 
GCGCTGCTTG AGACCAGCCT CCACAATGAA GATTCCACAC TAATCGTGCC CGTCGAGGGT 
GGCCGCTATG ATGTCAGCAT CAAGGAACGC ACCAAAACCC CCGTCTATTG GGAGGGCAAG 
GGCATTGAGG TGCGCCGCTG CTCCTGGTTC TACAAGGGCG TAGACTCCAA GTATGTGCCC 
TATTCTGAGG ACACGGCCGC ACTGCTGGAA GACGAGTACA AACGTTCGGC AGAGACTGGC 
GAATGGCATC AGAAGATCAT GCTGGCCAAC GGTGAACAGG TGGTCTTCCA TGGGCCCACA 
GTCATCGTGC ATTTCCTGCC ACAACAGAAT TCGGATTCGT GGGGGGCCAG CGCACAGAGC 
TCCATGCGTC CGCGGGTGGT CAAGAGGGAT CTGGATGACT TTGTCATCAA GCAGGGCGAG 
TCCCAGCGCG TGGATCATCT GTTGTTTATG GTCCATGGCA TTGGTTCGGC CTGTGATCTG 
AAAATGCGTT CAGTGGAGGA AGTGGTGGAT GATTTCAGAG ACATTGCCCA GCAGCTGGTT 
CAGTCGCACT ACAAGAACTC CACAGATATG GGCCTGGTAG GTCGCGTCGA AGTGTTGCCC 
ATCTCCTGGC ATGGCCATCT ACACTCCGAG GAGGAGGGCA TCGATGAGAA GCTAAAATCC 
ATTACCCTAG AGTCGATACC ACGGCTACGT AACTTTACGA ACGACACTCT GCTGGACGTA 
CTCTTCTACA CCAGCCCCAA GTATTGTCAG AAGATTATGA ACACGGTGGC CGATGCTCTC 
AATGAGGTCT ATCTGAAGTA CCGATTCAGG CATCCGGAAT TCAACGGCGG CGTCTCATTG 
GCCGGCCACA GTCTGGGGTC ACTGATCCTA TTCGATCTGC TGTGCCATCA GGAGCCACTG 
AAAGAGAGCG AAGAGGAGAA TAAGGAGAAC CCAGACCAGC TGCCGCAGAA GAACGAAAAC 
AACTACAAGA AAGTGCAACT GCCCTCCGGC GATCTGCTGC CGAAGCAGGT CAGCTACACC 
ATGGGTCCGG CCGGCACTGG ACAGCCGGTC ATCAACTACA CACAGCTCAT CTTCCACCCG 
AAGAAGTTCT TTGCCCTGGG CTCGCCAATC GGCATGTTTG TGACCATTCG GGGCATCGAT 
CAGCTTGGCC TGGACTTTCA CCTGCCCACA TGTCCGGGTT TCTACAATAT ATTCCATCCC 
TTCGATCCGG TGGCCTATCG GATTGAGGCT CTCGTCAATG CGGATATGAA TGGTATACGT 
CCTGTTCTCA TACCCCATCA CAAGGGACGC AAGCGCATGC ATCTGGAGCT GAGGGAGACT 
ATGACGCGTG TGGGGGCAGA TATCAAGCAG AGATTCATGG ACACATTCAA GACCACCCTG 
GACAGTGTGA ACTTCTTGGC CACGGTGACC AAAGTTAAAA AGGAGGCAGA AGAGACGCTC 
GAAAAGCAGG TCTTGAATCA GACTTCGTCG CAGTTCCAGA AAGAAAACGA TGATCAAGAG 
GATATTTCTG TGGCCAGCAC ATCCACAAAG ACAAGAAATC GCACATATTC CAACTCGACA 
ACGGCCTCAG ATCCAGAGTT TGTAGAACTG GACTTTCCGC TAGGCAAACT GAACGATTCG 
AAGCGCGTCG ACTATGTGCT GCAAGAGGCA CCGCTGGAGT TTATCAATGA GTACATCTTT 
GCCTTGAGCA GTCATGTCTG CTACTGGGGC TCTGAAGACA CAATCCTGTT TGTGATGAAG 
GAAATCTATG CCAGCCTCGG GATCAGCACC GACAGCCAGG TGCCCCAGCA GTCCATGACC 
ATCGAGAGGC CCGGCTCATT TGACAGTAGT CAGTCGCTGT TGCCGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001356520.2
CDS	19..5829
Translation	MSERSKGDVSRVNNNPNAPRVVKNPLLSASVTGLSYEDFPNKPQLLPAAPPVVHAPPPASTPVLIAGILNREPKTLVTVGQPSSIDDSEALDEINLNTSSEDSSGVSGGSSTAYLRGTGDGNANPLLEPPPGAGNDSLLSQAASSFTALPSVASNVFSTFSKRIYAGTKESEEPKLDIQPTYIQQAGHYAPPPVAAAPPPPFYAAPPAAANEIVAAPEPPKFYAPTEVPAPNVGIASAPPPTASGNPNTYRFTARKKLYAPIPGFTEQSAQGPQIPQAPQAGLPFQTPPYPAQQAAPFEISSASPFEIQAAPPAAAATATEERKSGGGLFSLSNLVPTGVLQNISGLVQSATGRSSEQSSSNPPPSGGGYFDINTVAPANPSNYFGGPPAAAPPAGSYFVPGAVPEAATAIPQPPVGGFFPQPANPIPTAAPTSVAAPPTVGGFFGAIPPANPPATDINPISGAPPVGGFFTPGVAAVPPTVGGFYTPVSAPIEINPIAEAPPPAEAAIPPSSALAGGFTATPGAVPSVTTSTTAAPPPIGGFFDPNPAASGIPPTSTVGGFFTPAAVPPVATAAPVPPVATVTAAAPPPVGGFFAPAGAGPPSNATPLAGPPLGTAPPLAGPPLGSAATVAGPPPAAGQASYRLQKGTRLYKSPLTAQETATASAFGTPFATTAAVPAAVFNPFGTGASGVFNPLAGNPPVGQQENEDNSAVALFAPPSAPVAGQTLDQGPAPPAAFASTNQGSPFPAPQPGPQATPFFIPQTGDRPPVPTSQETAPSSQGISFFEAPPTSDIALFTPPPVQETAAAPVETQGISFFAPQPDLAPKAAQEIPLFVPAAVEAPPEGHPLYPPTSTAVAEDIPAAESPLFVPVPTSAADQSQGVPLYPPETATSNASSSVLFAASEHQPSAPLFEAVQPPTTEAVDASASDFFGSANAEAVPASANTETAYSPFGQTVTTPSQVLQTPPAQDLLPPPIGFVAPQQPEAEPEPEVAAAAPPAEFGGVTEALALAHPPLAPSPLQSFFSQPATAAPEDFNFFAAPGSSLFPELPQQRGIAPPPLAQEPPPAAAESGIAPGLGFDQFLSQSQQAQSHNYIGNSNENSYQQNSSVNTFSGYFGGPTVTEAPAPPPATTTAAAVPLAAPVSNCDPSHFFDQVPQPQAPTNANEDQRIQNFFNNPPLQDQPAAPGELKYDIVHSGLPVKQLQARSQTPISNLVEPPSSSACSEFSTLAPAAPTAPATAPATNPTVERQTGEDLLQLHQAELPEEILKELRMAANATTSDKAGTPPVSIVYTPAVVHWFYKRRVDAKHIWTPFSHYDSALLETSLHNEDSTLIVPVEGGRYDVSIKERTKTPVYWEGKGIEVRRCSWFYKGVDSKYVPYSEDTAALLEDEYKRSAETGEWHQKIMLANGEQVVFHGPTVIVHFLPQQNSDSWGASAQSSMRPRVVKRDLDDFVIKQGESQRVDHLLFMVHGIGSACDLKMRSVEEVVDDFRDIAQQLVQSHYKNSTDMGLVGRVEVLPISWHGHLHSEEEGIDEKLKSITLESIPRLRNFTNDTLLDVLFYTSPKYCQKIMNTVADALNEVYLKYRFRHPEFNGGVSLAGHSLGSLILFDLLCHQEPLKESEEENKENPDQLPQKNENNYKKVQLPSGDLLPKQVSYTMGPAGTGQPVINYTQLIFHPKKFFALGSPIGMFVTIRGIDQLGLDFHLPTCPGFYNIFHPFDPVAYRIEALVNADMNGIRPVLIPHHKGRKRMHLELRETMTRVGADIKQRFMDTFKTTLDSVNFLATVTKVKKEAEETLEKQVLNQTSSQFQKENDDQEDISVASTSTKTRNRTYSNSTTASDPEFVELDFPLGKLNDSKRVDYVLQEAPLEFINEYIFALSSHVCYWGSEDTILFVMKEIYASLGISTDSQVPQQSMTIERPGSFDSSQSLLPM

Target ORF information:

RefSeq Version	XM_001356484.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA21159), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001356484.3

ORF Insert Sequence:

ATGTCCGAAA GATCCAAAGG TGACGTGTCT CGCGTAAACA ACAATCCAAA TGCGCCGCGT 
GTCGTTAAAA ATCCATTACT TAGCGCCTCG GTAACCGGAC TCAGCTACGA AGACTTTCCC 
AACAAGCCGC AACTGTTGCC CGCCGCCCCG CCCGTGGTGC ATGCCCCGCC GCCAGCATCC 
ACACCCGTCC TGATCGCTGG CATTCTGAAT CGCGAGCCAA AAACTTTGGT GACCGTGGGA 
CAGCCATCGA GCATCGATGA CAGCGAAGCC CTCGACGAGA TCAACTTGAA TACGAGCAGC 
GAGGACTCGA GTGGAGTCAG TGGTGGATCG TCGACTGCGT ATCTGCGTGG CACAGGCGAT 
GGTAATGCCA ATCCCTTGCT AGAACCACCA CCGGGCGCTG GAAACGATTC GTTGTTGAGC 
CAGGCGGCCT CATCCTTCAC AGCACTGCCC TCGGTGGCCT CCAATGTGTT TTCCACCTTC 
TCCAAGAGAA TCTACGCGGG CACAAAGGAA TCGGAAGAGC CTAAATTGGA CATCCAGCCC 
ACGTACATAC AGCAGGCGGG ACACTACGCC CCACCGCCAG TAGCTGCAGC ACCACCACCA 
CCATTCTACG CGGCACCACC GGCGGCAGCG AATGAGATTG TTGCTGCCCC AGAACCACCC 
AAGTTCTATG CCCCCACGGA GGTGCCAGCC CCGAACGTAG GCATCGCCAG TGCACCACCA 
CCCACCGCAT CGGGAAATCC CAACACCTAT CGATTCACCG CCAGAAAGAA GCTATACGCA 
CCGATTCCTG GCTTCACCGA GCAGTCGGCA CAGGGTCCGC AGATTCCACA GGCCCCACAG 
GCAGGTCTGC CCTTCCAGAC GCCACCATAT CCAGCCCAAC AGGCAGCTCC ATTTGAGATA 
TCATCAGCAT CTCCTTTTGA GATTCAAGCA GCTCCCCCAG CAGCAGCAGC GACTGCCACA 
GAGGAGCGCA AATCCGGCGG AGGACTCTTT TCTCTGAGCA ATCTTGTGCC CACTGGCGTG 
CTGCAAAACA TCTCCGGACT TGTACAGTCC GCCACAGGCA GGAGCAGCGA GCAGTCCTCT 
AGTAATCCTC CACCATCTGG CGGGGGCTAC TTTGACATCA ACACAGTCGC TCCAGCAAAT 
CCGAGCAACT ATTTCGGTGG CCCACCAGCG GCGGCACCGC CAGCGGGAAG CTACTTTGTG 
CCTGGCGCAG TTCCTGAGGC AGCGACAGCA ATTCCTCAAC CACCAGTAGG TGGGTTCTTT 
CCACAGCCAG CCAATCCGAT ACCGACAGCT GCTCCCACTT CAGTCGCGGC TCCTCCCACA 
GTTGGAGGAT TCTTTGGGGC AATACCACCT GCTAATCCGC CAGCAACTGA TATCAATCCC 
ATCTCGGGAG CACCACCAGT TGGAGGATTC TTTACACCAG GTGTAGCAGC AGTTCCTCCC 
ACAGTTGGAG GATTTTATAC GCCCGTTTCG GCACCGATTG AGATCAATCC TATTGCTGAA 
GCACCACCAC CGGCAGAAGC AGCTATTCCC CCATCATCTG CACTTGCAGG AGGATTTACT 
GCAACCCCAG GAGCAGTGCC ATCTGTGACT ACATCCACGA CAGCAGCTCC TCCGCCAATT 
GGAGGGTTCT TTGATCCTAA TCCAGCAGCA TCTGGTATTC CTCCCACTTC CACAGTTGGA 
GGATTCTTTA CGCCAGCAGC AGTTCCACCT GTGGCCACAG CAGCACCTGT TCCACCTGTT 
GCCACAGTAA CAGCAGCAGC TCCTCCACCA GTAGGAGGAT TCTTTGCACC CGCAGGAGCA 
GGGCCACCAT CCAACGCAAC TCCACTGGCA GGACCACCTC TGGGGACAGC ACCTCCATTG 
GCAGGACCAC CTTTAGGATC AGCAGCAACT GTCGCTGGAC CACCACCTGC CGCTGGACAG 
GCATCGTATC GCCTCCAGAA GGGCACACGT CTCTACAAGA GTCCATTGAC GGCACAGGAA 
ACTGCCACAG CGTCTGCTTT CGGCACTCCC TTTGCCACAA CAGCAGCTGT CCCAGCTGCC 
GTGTTCAATC CATTTGGAAC TGGAGCTTCA GGAGTATTTA ATCCATTAGC TGGCAACCCA 
CCAGTTGGTC AGCAGGAGAA CGAAGATAAC TCAGCCGTTG CACTATTTGC ACCTCCTTCT 
GCACCAGTGG CAGGACAGAC CTTAGATCAA GGACCGGCTC CTCCAGCTGC CTTTGCATCG 
ACAAATCAGG GAAGTCCCTT CCCCGCTCCA CAGCCAGGAC CCCAGGCAAC GCCATTCTTC 
ATTCCACAAA CGGGGGACAG ACCTCCAGTA CCCACCAGCC AGGAAACTGC ACCATCCAGC 
CAAGGAATAT CCTTCTTTGA AGCACCTCCG ACATCGGACA TTGCTCTCTT CACACCGCCA 
CCCGTCCAGG AAACAGCAGC AGCACCAGTC GAAACACAAG GAATATCTTT CTTTGCACCG 
CAGCCAGATC TCGCACCGAA AGCAGCCCAA GAGATTCCAC TGTTTGTCCC GGCAGCAGTT 
GAGGCACCTC CAGAAGGACA TCCCTTGTAT CCACCGACAT CTACAGCAGT CGCAGAGGAC 
ATTCCTGCCG CTGAGAGCCC ATTATTTGTG CCAGTGCCAA CATCGGCAGC AGATCAGAGT 
CAGGGAGTAC CGCTCTATCC ACCAGAAACA GCCACATCTA ACGCTTCATC TAGCGTTCTC 
TTTGCTGCAT CGGAGCATCA ACCGAGTGCA CCATTATTCG AAGCTGTTCA ACCACCAACC 
ACTGAGGCAG TCGATGCTTC AGCAAGCGAT TTCTTTGGAT CTGCCAATGC TGAAGCTGTC 
CCTGCATCTG CAAATACCGA AACGGCTTAC AGCCCATTCG GACAGACGGT GACCACACCC 
TCGCAAGTGC TGCAGACACC ACCGGCACAA GATTTACTGC CACCGCCTAT TGGATTTGTT 
GCGCCACAAC AACCAGAGGC AGAGCCAGAG CCAGAGGTGG CCGCCGCCGC TCCTCCAGCA 
GAATTCGGAG GAGTTACCGA AGCACTAGCC CTAGCCCACC CACCACTTGC ACCATCGCCG 
CTTCAGAGCT TCTTTTCGCA GCCAGCGACT GCTGCCCCAG AGGATTTCAA CTTTTTTGCC 
GCACCAGGCA GCAGCCTGTT TCCAGAACTG CCACAGCAGC GGGGCATAGC GCCGCCCCCT 
TTGGCACAGG AGCCACCGCC GGCAGCAGCA GAATCTGGTA TTGCACCAGG ACTGGGCTTT 
GATCAATTTC TAAGCCAATC CCAGCAAGCG CAAAGCCACA ACTACATTGG AAATTCAAAT 
GAGAATTCCT ATCAACAAAA TTCAAGTGTC AATACATTCT CCGGCTATTT TGGTGGCCCC 
ACTGTGACAG AAGCCCCAGC CCCACCCCCA GCCACAACCA CAGCAGCAGC AGTGCCACTT 
GCTGCTCCAG TTAGTAACTG TGACCCCTCC CATTTCTTTG ATCAAGTGCC ACAGCCACAG 
GCCCCGACAA ACGCTAACGA GGATCAACGC ATACAGAACT TCTTCAACAA CCCGCCACTG 
CAGGATCAGC CAGCGGCACC GGGCGAACTC AAATACGATA TAGTGCACAG CGGTCTGCCC 
GTCAAACAGC TACAGGCACG CTCCCAGACA CCCATCTCCA ATTTGGTGGA GCCCCCATCC 
TCGTCTGCCT GCTCAGAGTT TTCGACACTG GCACCAGCAG CACCCACAGC TCCAGCAACA 
GCTCCGGCAA CCAATCCAAC AGTCGAGCGT CAAACGGGCG AGGATCTACT GCAGTTGCAT 
CAAGCGGAAT TGCCGGAGGA GATCTTGAAA GAGCTCAGAA TGGCCGCAAA TGCAACGACA 
AGCGACAAGG CAGGCACACC CCCTGTTTCT ATAGTTTATA CGCCTGCTGT GGTCCATTGG 
TTCTACAAAC GTAGAGTGGA CGCGAAACAT ATTTGGACAC CATTCAGTCA CTATGATTCA 
GCGCTGCTTG AGACCAGCCT CCACAATGAA GATTCCACAC TAATCGTGCC CGTCGAGGGT 
GGCCGCTATG ATGTCAGCAT CAAGGAACGC ACCAAAACCC CCGTCTATTG GGAGGGCAAG 
GGCATTGAGG TGCGCCGCTG CTCCTGGTTC TACAAGGGCG TAGACTCCAA GTATGTGCCC 
TATTCTGAGG ACACGGCCGC ACTGCTGGAA GACGAGTACA AACGTTCGGC AGAGACTGGC 
GAATGGCATC AGAAGATCAT GCTGGCCAAC GGTGAACAGG TGGTCTTCCA TGGGCCCACA 
GTCATCGTGC ATTTCCTGCC ACAACAGAAT TCGGATTCGT GGGGGGCCAG CGCACAGAGC 
TCCATGCGTC CGCGGGTGGT CAAGAGGGAT CTGGATGACT TTGTCATCAA GCAGGGCGAG 
TCCCAGCGCG TGGATCATCT GTTGTTTATG GTCCATGGCA TTGGTTCGGC CTGTGATCTG 
AAAATGCGTT CAGTGGAGGA AGTGGTGGAT GATTTCAGAG ACATTGCCCA GCAGCTGGTT 
CAGTCGCACT ACAAGAACTC CACAGATATG GGCCTGGTAG GTCGCGTCGA AGTGTTGCCC 
ATCTCCTGGC ATGGCCATCT ACACTCCGAG GAGGAGGGCA TCGATGAGAA GCTAAAATCC 
ATTACCCTAG AGTCGATACC ACGGCTACGT AACTTTACGA ACGACACTCT GCTGGACGTA 
CTCTTCTACA CCAGCCCCAA GTATTGTCAG AAGATTATGA ACACGGTGGC CGATGCTCTC 
AATGAGGTCT ATCTGAAGTA CCGATTCAGG CATCCGGAAT TCAACGGCGG CGTCTCATTG 
GCCGGCCACA GTCTGGGGTC ACTGATCCTA TTCGATCTGC TGTGCCATCA GGAGCCACTG 
AAAGAGAGCG AAGAGGAGAA TAAGGAGAAC CCAGACCAGC TGCCGCAGAA GAACGAAAAC 
AACTACAAGA AAGTGCAACT GCCCTCCGGC GATCTGCTGC CGAAGCAGGT CAGCTACACC 
ATGGGTCCGG CCGGCACTGG ACAGCCGGTC ATCAACTACA CACAGCTCAT CTTCCACCCG 
AAGAAGTTCT TTGCCCTGGG CTCGCCAATC GGCATGTTTG TGACCATTCG GGGCATCGAT 
CAGCTTGGCC TGGACTTTCA CCTGCCCACA TGTCCGGGTT TCTACAATAT ATTCCATCCC 
TTCGATCCGG TGGCCTATCG GATTGAGGCT CTCGTCAATG CGGATATGAA TGGTATACGT 
CCTGTTCTCA TACCCCATCA CAAGGGACGC AAGCGCATGC ATCTGGAGCT GAGGGAGACT 
ATGACGCGTG TGGGGGCAGA TATCAAGCAG AGATTCATGG ACACATTCAA GACCACCCTG 
GACAGTGTGA ACTTCTTGGC CACGGTGACC AAAGTTAAAA AGGAGGCAGA AGAGACGCTC 
GAAAAGCAGG TCTTGAATCA GACTTCGTCG CAGTTCCAGA AAGAAAACGA TGATCAAGAG 
GATATTTCTG TGGCCAGCAC ATCCACAAAG ACAAGAAATC GCACATATTC CAACTCGACA 
ACGGCCTCAG ATCCAGAGTT TGTAGAACTG GACTTTCCGC TAGGCAAACT GAACGATTCG 
AAGCGCGTCG ACTATGTGCT GCAAGAGGCA CCGCTGGAGT TTATCAATGA GTACATCTTT 
GCCTTGAGCA GTCATGTCTG CTACTGGGGC TCTGAAGACA CAATCCTGTT TGTGATGAAG 
GAAATCTATG CCAGCCTCGG GATCAGCACC GACAGCCAGG TGCCCCAGCA GTCCATGACC 
ATCGAGAGGC CCGGCTCATT TGACAGTAGT CAGTCGCTGT TGCCGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA21159 ( XM_015180406.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015035892.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA21159), mRNA.

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CloneID	ODe17804
Clone ID Related Accession (Same CDS sequence)	XM_015180406.1
Accession Version	XM_015180406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589964). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAAA GATCCAAAGG TGACGTGTCT CGCGTAAACA ACAATCCAAA TGCGCCGCGT 
GTCGTTAAAA ATCCATTACT TAGCGCCTCG GTAACCGGAC TCAGCTACGA AGACTTTCCC 
AACAAGCCGC AACTGTTGCC CGCCGCCCCG CCCGTGGTGC ATGCCCCGCC GCCAGCATCC 
ACACCCGTCC TGATCGCTGG CATTCTGAAT CGCGAGCCAA AAACTTTGGT GACCGTGGGA 
CAGCCATCGA GCATCGATGA CAGCGAAGCC CTCGACGAGA TCAACTTGAA TACGAGCAGC 
GAGGACTCGA GTGGAGTCAG TGGTGGATCG TCGACTGCGT ATCTGCGTGG CACAGGCGAT 
GGTAATGCCA ATCCCTTGCT AGAACCACCA CCGGGCGCTG GAAACGATTC GTTGTTGAGC 
CAGGCGGCCT CATCCTTCAC AGCACTGCCC TCGGTGGCCT CCAATGTGTT TTCCACCTTC 
TCCAAGAGAA TCTACGCGGG CACAAAGGAA TCGGAAGAGC CTAAATTGGA CATCCAGCCC 
ACGTACATAC AGCAGGCGGG ACACTACGCC CCACCGCCAG TAGCTGCAGC ACCACCACCA 
CCATTCTACG CGGCACCACC GGCGGCAGCG AATGAGATTG TTGCTGCCCC AGAACCACCC 
AAGTTCTATG CCCCCACGGA GGTGCCAGCC CCGAACGTAG GCATCGCCAG TGCACCACCA 
CCCACCGCAT CGGGAAATCC CAACACCTAT CGATTCACCG CCAGAAAGAA GCTATACGCA 
CCGATTCCTG GCTTCACCGA GCAGTCGGCA CAGGGTCCGC AGATTCCACA GGCCCCACAG 
GCAGGTCTGC CCTTCCAGAC GCCACCATAT CCAGCCCAAC AGGCAGCTCC ATTTGAGATA 
TCATCAGCAT CTCCTTTTGA GATTCAAGCA GCTCCCCCAG CAGCAGCAGC GACTGCCACA 
GAGGAGCGCA AATCCGGCGG AGGACTCTTT TCTCTGAGCA ATCTTGTGCC CACTGGCGTG 
CTGCAAAACA TCTCCGGACT TGTACAGTCC GCCACAGGCA GGAGCAGCGA GCAGTCCTCT 
AGTAATCCTC CACCATCTGG CGGGGGCTAC TTTGACATCA ACACAGTCGC TCCAGCAAAT 
CCGAGCAACT ATTTCGGTGG CCCACCAGCG GCGGCACCGC CAGCGGGAAG CTACTTTGTG 
CCTGGCGCAG TTCCTGAGGC AGCGACAGCA ATTCCTCAAC CACCAGTAGG TGGGTTCTTT 
CCACAGCCAG CCAATCCGAT ACCGACAGCT GCTCCCACTT CAGTCGCGGC TCCTCCCACA 
GTTGGAGGAT TCTTTGGGGC AATACCACCT GCTAATCCGC CAGCAACTGA TATCAATCCC 
ATCTCGGGAG CACCACCAGT TGGAGGATTC TTTACACCAG GTGTAGCAGC AGTTCCTCCC 
ACAGTTGGAG GATTTTATAC GCCCGTTTCG GCACCGATTG AGATCAATCC TATTGCTGAA 
GCACCACCAC CGGCAGAAGC AGCTATTCCC CCATCATCTG CACTTGCAGG AGGATTTACT 
GCAACCCCAG GAGCAGTGCC ATCTGTGACT ACATCCACGA CAGCAGCTCC TCCGCCAATT 
GGAGGGTTCT TTGATCCTAA TCCAGCAGCA TCTGGTATTC CTCCCACTTC CACAGTTGGA 
GGATTCTTTA CGCCAGCAGC AGTTCCACCT GTGGCCACAG CAGCACCTGT TCCACCTGTT 
GCCACAGTAA CAGCAGCAGC TCCTCCACCA GTAGGAGGAT TCTTTGCACC CGCAGGAGCA 
GGGCCACCAT CCAACGCAAC TCCACTGGCA GGACCACCTC TGGGGACAGC ACCTCCATTG 
GCAGGACCAC CTTTAGGATC AGCAGCAACT GTCGCTGGAC CACCACCTGC CGCTGGACAG 
GCATCGTATC GCCTCCAGAA GGGCACACGT CTCTACAAGA GTCCATTGAC GGCACAGGAA 
ACTGCCACAG CGTCTGCTTT CGGCACTCCC TTTGCCACAA CAGCAGCTGT CCCAGCTGCC 
GTGTTCAATC CATTTGGAAC TGGAGCTTCA GGAGTATTTA ATCCATTAGC TGGCAACCCA 
CCAGTTGGTC AGCAGGAGAA CGAAGATAAC TCAGCCGTTG CACTATTTGC ACCTCCTTCT 
GCACCAGTGG CAGGACAGAC CTTAGATCAA GGACCGGCTC CTCCAGCTGC CTTTGCATCG 
ACAAATCAGG GAAGTCCCTT CCCCGCTCCA CAGCCAGGAC CCCAGGCAAC GCCATTCTTC 
ATTCCACAAA CGGGGGACAG ACCTCCAGTA CCCACCAGCC AGGAAACTGC ACCATCCAGC 
CAAGGAATAT CCTTCTTTGA AGCACCTCCG ACATCGGACA TTGCTCTCTT CACACCGCCA 
CCCGTCCAGG AAACAGCAGC AGCACCAGTC GAAACACAAG GAATATCTTT CTTTGCACCG 
CAGCCAGATC TCGCACCGAA AGCAGCCCAA GAGATTCCAC TGTTTGTCCC GGCAGCAGTT 
GAGGCACCTC CAGAAGGACA TCCCTTGTAT CCACCGACAT CTACAGCAGT CGCAGAGGAC 
ATTCCTGCCG CTGAGAGCCC ATTATTTGTG CCAGTGCCAA CATCGGCAGC AGATCAGAGT 
CAGGGAGTAC CGCTCTATCC ACCAGAAACA GCCACATCTA ACGCTTCATC TAGCGTTCTC 
TTTGCTGCAT CGGAGCATCA ACCGAGTGCA CCATTATTCG AAGCTGTTCA ACCACCAACC 
ACTGAGGCAG TCGATGCTTC AGCAAGCGAT TTCTTTGGAT CTGCCAATGC TGAAGCTGTC 
CCTGCATCTG CAAATACCGA AACGGCTTAC AGCCCATTCG GACAGACGGT GACCACACCC 
TCGCAAGTGC TGCAGACACC ACCGGCACAA GATTTACTGC CACCGCCTAT TGGATTTGTT 
GCGCCACAAC AACCAGAGGC AGAGCCAGAG CCAGAGGTGG CCGCCGCCGC TCCTCCAGCA 
GAATTCGGAG GAGTTACCGA AGCACTAGCC CTAGCCCACC CACCACTTGC ACCATCGCCG 
CTTCAGAGCT TCTTTTCGCA GCCAGCGACT GCTGCCCCAG AGGATTTCAA CTTTTTTGCC 
GCACCAGGCA GCAGCCTGTT TCCAGAACTG CCACAGCAGC GGGGCATAGC GCCGCCCCCT 
TTGGCACAGG AGCCACCGCC GGCAGCAGCA GAATCTGGTA TTGCACCAGG ACTGGGCTTT 
GATCAATTTC TAAGCCAATC CCAGCAAGCG CAAAGCCACA ACTACATTGG AAATTCAAAT 
GAGAATTCCT ATCAACAAAA TTCAAGTGTC AATACATTCT CCGGCTATTT TGGTGGCCCC 
ACTGTGACAG AAGCCCCAGC CCCACCCCCA GCCACAACCA CAGCAGCAGC AGTGCCACTT 
GCTGCTCCAG TTAGTAACTG TGACCCCTCC CATTTCTTTG ATCAAGTGCC ACAGCCACAG 
GCCCCGACAA ACGCTAACGA GGATCAACGC ATACAGAACT TCTTCAACAA CCCGCCACTG 
CAGGATCAGC CAGCGGCACC GGGCGAACTC AAATACGATA TAGTGCACAG CGGTCTGCCC 
GTCAAACAGC TACAGGCACG CTCCCAGACA CCCATCTCCA ATTTGGTGGA GCCCCCATCC 
TCGTCTGCCT GCTCAGAGTT TTCGACACTG GCACCAGCAG CACCCACAGC TCCAGCAACA 
GCTCCGGCAA CCAATCCAAC AGTCGAGCGT CAAACGGGCG AGGATCTACT GCAGTTGCAT 
CAAGCGGAAT TGCCGGAGGA GATCTTGAAA GAGCTCAGAA TGGCCGCAAA TGCAACGACA 
AGCGACAAGG CAGGCACACC CCCTGTTTCT ATAGTTTATA CGCCTGCTGT GGTCCATTGG 
TTCTACAAAC GTAGAGTGGA CGCGAAACAT ATTTGGACAC CATTCAGTCA CTATGATTCA 
GCGCTGCTTG AGACCAGCCT CCACAATGAT TCCACACTAA TCGTGCCCGT CGAGGGTGGC 
CGCTATGATG TCAGCATCAA GGAACGCACC AAAACCCCCG TCTATTGGGA GGGCAAGGGC 
ATTGAGGTGC GCCGCTGCTC CTGGTTCTAC AAGGGCGTAG ACTCCAAGTA TGTGCCCTAT 
TCTGAGGACA CGGCCGCACT GCTGGAAGAC GAGTACAAAC GTTCGGCAGA GACTGGCGAA 
TGGCATCAGA AGATCATGCT GGCCAACGGT GAACAGGTGG TCTTCCATGG GCCCACAGTC 
ATCGTGCATT TCCTGCCACA ACAGAATTCG GATTCGTGGG GGGCCAGCGC ACAGAGCTCC 
ATGCGTCCGC GGGTGGTCAA GAGGGATCTG GATGACTTTG TCATCAAGCA GGGCGAGTCC 
CAGCGCGTGG ATCATCTGTT GTTTATGGTC CATGGCATTG GTTCGGCCTG TGATCTGAAA 
ATGCGTTCAG TGGAGGAAGT GGTGGATGAT TTCAGAGACA TTGCCCAGCA GCTGGTTCAG 
TCGCACTACA AGAACTCCAC AGATATGGGC CTGGTAGGTC GCGTCGAAGT GTTGCCCATC 
TCCTGGCATG GCCATCTACA CTCCGAGGAG GAGGGCATCG ATGAGAAGCT AAAATCCATT 
ACCCTAGAGT CGATACCACG GCTACGTAAC TTTACGAACG ACACTCTGCT GGACGTACTC 
TTCTACACCA GCCCCAAGTA TTGTCAGAAG ATTATGAACA CGGTGGCCGA TGCTCTCAAT 
GAGGTCTATC TGAAGTACCG ATTCAGGCAT CCGGAATTCA ACGGCGGCGT CTCATTGGCC 
GGCCACAGTC TGGGGTCACT GATCCTATTC GATCTGCTGT GCCATCAGGA GCCACTGAAA 
GAGAGCGAAG AGGAGAATAA GGAGAACCCA GACCAGCTGC CGCAGAAGAA CGAAAACAAC 
TACAAGAAAG TGCAACTGCC CTCCGGCGAT CTGCTGCCGA AGCAGGTCAG CTACACCATG 
GGTCCGGCCG GCACTGGACA GCCGGTCATC AACTACACAC AGCTCATCTT CCACCCGAAG 
AAGTTCTTTG CCCTGGGCTC GCCAATCGGC ATGTTTGTGA CCATTCGGGG CATCGATCAG 
CTTGGCCTGG ACTTTCACCT GCCCACATGT CCGGGTTTCT ACAATATATT CCATCCCTTC 
GATCCGGTGG CCTATCGGAT TGAGGCTCTC GTCAATGCGG ATATGAATGG TATACGTCCT 
GTTCTCATAC CCCATCACAA GGGACGCAAG CGCATGCATC TGGAGCTGAG GGAGACTATG 
ACGCGTGTGG GGGCAGATAT CAAGCAGAGA TTCATGGACA CATTCAAGAC CACCCTGGAC 
AGTGTGAACT TCTTGGCCAC GGTGACCAAA GTTAAAAAGG AGGCAGAAGA GACGCTCGAA 
AAGCAGGTCT TGAATCAGAC TTCGTCGCAG TTCCAGAAAG AAAACGATGA TCAAGAGGAT 
ATTTCTGTGG CCAGCACATC CACAAAGACA AGAAATCGCA CATATTCCAA CTCGACAACG 
GCCTCAGATC CAGAGTTTGT AGAACTGGAC TTTCCGCTAG GCAAACTGAA CGATTCGAAG 
CGCGTCGACT ATGTGCTGCA AGAGGCACCG CTGGAGTTTA TCAATGAGTA CATCTTTGCC 
TTGAGCAGTC ATGTCTGCTA CTGGGGCTCT GAAGACACAA TCCTGTTTGT GATGAAGGAA 
ATCTATGCCA GCCTCGGGAT CAGCACCGAC AGCCAGGTGC CCCAGCAGTC CATGACCATC 
GAGAGGCCCG GCTCATTTGA CAGTAGTCAG TCGCTGTTGC CGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015035892.1
CDS	19..5826
Translation	MSERSKGDVSRVNNNPNAPRVVKNPLLSASVTGLSYEDFPNKPQLLPAAPPVVHAPPPASTPVLIAGILNREPKTLVTVGQPSSIDDSEALDEINLNTSSEDSSGVSGGSSTAYLRGTGDGNANPLLEPPPGAGNDSLLSQAASSFTALPSVASNVFSTFSKRIYAGTKESEEPKLDIQPTYIQQAGHYAPPPVAAAPPPPFYAAPPAAANEIVAAPEPPKFYAPTEVPAPNVGIASAPPPTASGNPNTYRFTARKKLYAPIPGFTEQSAQGPQIPQAPQAGLPFQTPPYPAQQAAPFEISSASPFEIQAAPPAAAATATEERKSGGGLFSLSNLVPTGVLQNISGLVQSATGRSSEQSSSNPPPSGGGYFDINTVAPANPSNYFGGPPAAAPPAGSYFVPGAVPEAATAIPQPPVGGFFPQPANPIPTAAPTSVAAPPTVGGFFGAIPPANPPATDINPISGAPPVGGFFTPGVAAVPPTVGGFYTPVSAPIEINPIAEAPPPAEAAIPPSSALAGGFTATPGAVPSVTTSTTAAPPPIGGFFDPNPAASGIPPTSTVGGFFTPAAVPPVATAAPVPPVATVTAAAPPPVGGFFAPAGAGPPSNATPLAGPPLGTAPPLAGPPLGSAATVAGPPPAAGQASYRLQKGTRLYKSPLTAQETATASAFGTPFATTAAVPAAVFNPFGTGASGVFNPLAGNPPVGQQENEDNSAVALFAPPSAPVAGQTLDQGPAPPAAFASTNQGSPFPAPQPGPQATPFFIPQTGDRPPVPTSQETAPSSQGISFFEAPPTSDIALFTPPPVQETAAAPVETQGISFFAPQPDLAPKAAQEIPLFVPAAVEAPPEGHPLYPPTSTAVAEDIPAAESPLFVPVPTSAADQSQGVPLYPPETATSNASSSVLFAASEHQPSAPLFEAVQPPTTEAVDASASDFFGSANAEAVPASANTETAYSPFGQTVTTPSQVLQTPPAQDLLPPPIGFVAPQQPEAEPEPEVAAAAPPAEFGGVTEALALAHPPLAPSPLQSFFSQPATAAPEDFNFFAAPGSSLFPELPQQRGIAPPPLAQEPPPAAAESGIAPGLGFDQFLSQSQQAQSHNYIGNSNENSYQQNSSVNTFSGYFGGPTVTEAPAPPPATTTAAAVPLAAPVSNCDPSHFFDQVPQPQAPTNANEDQRIQNFFNNPPLQDQPAAPGELKYDIVHSGLPVKQLQARSQTPISNLVEPPSSSACSEFSTLAPAAPTAPATAPATNPTVERQTGEDLLQLHQAELPEEILKELRMAANATTSDKAGTPPVSIVYTPAVVHWFYKRRVDAKHIWTPFSHYDSALLETSLHNDSTLIVPVEGGRYDVSIKERTKTPVYWEGKGIEVRRCSWFYKGVDSKYVPYSEDTAALLEDEYKRSAETGEWHQKIMLANGEQVVFHGPTVIVHFLPQQNSDSWGASAQSSMRPRVVKRDLDDFVIKQGESQRVDHLLFMVHGIGSACDLKMRSVEEVVDDFRDIAQQLVQSHYKNSTDMGLVGRVEVLPISWHGHLHSEEEGIDEKLKSITLESIPRLRNFTNDTLLDVLFYTSPKYCQKIMNTVADALNEVYLKYRFRHPEFNGGVSLAGHSLGSLILFDLLCHQEPLKESEEENKENPDQLPQKNENNYKKVQLPSGDLLPKQVSYTMGPAGTGQPVINYTQLIFHPKKFFALGSPIGMFVTIRGIDQLGLDFHLPTCPGFYNIFHPFDPVAYRIEALVNADMNGIRPVLIPHHKGRKRMHLELRETMTRVGADIKQRFMDTFKTTLDSVNFLATVTKVKKEAEETLEKQVLNQTSSQFQKENDDQEDISVASTSTKTRNRTYSNSTTASDPEFVELDFPLGKLNDSKRVDYVLQEAPLEFINEYIFALSSHVCYWGSEDTILFVMKEIYASLGISTDSQVPQQSMTIERPGSFDSSQSLLPM

Target ORF information:

RefSeq Version	XM_015180406.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA21159), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015180406.1

ORF Insert Sequence:

ATGTCCGAAA GATCCAAAGG TGACGTGTCT CGCGTAAACA ACAATCCAAA TGCGCCGCGT 
GTCGTTAAAA ATCCATTACT TAGCGCCTCG GTAACCGGAC TCAGCTACGA AGACTTTCCC 
AACAAGCCGC AACTGTTGCC CGCCGCCCCG CCCGTGGTGC ATGCCCCGCC GCCAGCATCC 
ACACCCGTCC TGATCGCTGG CATTCTGAAT CGCGAGCCAA AAACTTTGGT GACCGTGGGA 
CAGCCATCGA GCATCGATGA CAGCGAAGCC CTCGACGAGA TCAACTTGAA TACGAGCAGC 
GAGGACTCGA GTGGAGTCAG TGGTGGATCG TCGACTGCGT ATCTGCGTGG CACAGGCGAT 
GGTAATGCCA ATCCCTTGCT AGAACCACCA CCGGGCGCTG GAAACGATTC GTTGTTGAGC 
CAGGCGGCCT CATCCTTCAC AGCACTGCCC TCGGTGGCCT CCAATGTGTT TTCCACCTTC 
TCCAAGAGAA TCTACGCGGG CACAAAGGAA TCGGAAGAGC CTAAATTGGA CATCCAGCCC 
ACGTACATAC AGCAGGCGGG ACACTACGCC CCACCGCCAG TAGCTGCAGC ACCACCACCA 
CCATTCTACG CGGCACCACC GGCGGCAGCG AATGAGATTG TTGCTGCCCC AGAACCACCC 
AAGTTCTATG CCCCCACGGA GGTGCCAGCC CCGAACGTAG GCATCGCCAG TGCACCACCA 
CCCACCGCAT CGGGAAATCC CAACACCTAT CGATTCACCG CCAGAAAGAA GCTATACGCA 
CCGATTCCTG GCTTCACCGA GCAGTCGGCA CAGGGTCCGC AGATTCCACA GGCCCCACAG 
GCAGGTCTGC CCTTCCAGAC GCCACCATAT CCAGCCCAAC AGGCAGCTCC ATTTGAGATA 
TCATCAGCAT CTCCTTTTGA GATTCAAGCA GCTCCCCCAG CAGCAGCAGC GACTGCCACA 
GAGGAGCGCA AATCCGGCGG AGGACTCTTT TCTCTGAGCA ATCTTGTGCC CACTGGCGTG 
CTGCAAAACA TCTCCGGACT TGTACAGTCC GCCACAGGCA GGAGCAGCGA GCAGTCCTCT 
AGTAATCCTC CACCATCTGG CGGGGGCTAC TTTGACATCA ACACAGTCGC TCCAGCAAAT 
CCGAGCAACT ATTTCGGTGG CCCACCAGCG GCGGCACCGC CAGCGGGAAG CTACTTTGTG 
CCTGGCGCAG TTCCTGAGGC AGCGACAGCA ATTCCTCAAC CACCAGTAGG TGGGTTCTTT 
CCACAGCCAG CCAATCCGAT ACCGACAGCT GCTCCCACTT CAGTCGCGGC TCCTCCCACA 
GTTGGAGGAT TCTTTGGGGC AATACCACCT GCTAATCCGC CAGCAACTGA TATCAATCCC 
ATCTCGGGAG CACCACCAGT TGGAGGATTC TTTACACCAG GTGTAGCAGC AGTTCCTCCC 
ACAGTTGGAG GATTTTATAC GCCCGTTTCG GCACCGATTG AGATCAATCC TATTGCTGAA 
GCACCACCAC CGGCAGAAGC AGCTATTCCC CCATCATCTG CACTTGCAGG AGGATTTACT 
GCAACCCCAG GAGCAGTGCC ATCTGTGACT ACATCCACGA CAGCAGCTCC TCCGCCAATT 
GGAGGGTTCT TTGATCCTAA TCCAGCAGCA TCTGGTATTC CTCCCACTTC CACAGTTGGA 
GGATTCTTTA CGCCAGCAGC AGTTCCACCT GTGGCCACAG CAGCACCTGT TCCACCTGTT 
GCCACAGTAA CAGCAGCAGC TCCTCCACCA GTAGGAGGAT TCTTTGCACC CGCAGGAGCA 
GGGCCACCAT CCAACGCAAC TCCACTGGCA GGACCACCTC TGGGGACAGC ACCTCCATTG 
GCAGGACCAC CTTTAGGATC AGCAGCAACT GTCGCTGGAC CACCACCTGC CGCTGGACAG 
GCATCGTATC GCCTCCAGAA GGGCACACGT CTCTACAAGA GTCCATTGAC GGCACAGGAA 
ACTGCCACAG CGTCTGCTTT CGGCACTCCC TTTGCCACAA CAGCAGCTGT CCCAGCTGCC 
GTGTTCAATC CATTTGGAAC TGGAGCTTCA GGAGTATTTA ATCCATTAGC TGGCAACCCA 
CCAGTTGGTC AGCAGGAGAA CGAAGATAAC TCAGCCGTTG CACTATTTGC ACCTCCTTCT 
GCACCAGTGG CAGGACAGAC CTTAGATCAA GGACCGGCTC CTCCAGCTGC CTTTGCATCG 
ACAAATCAGG GAAGTCCCTT CCCCGCTCCA CAGCCAGGAC CCCAGGCAAC GCCATTCTTC 
ATTCCACAAA CGGGGGACAG ACCTCCAGTA CCCACCAGCC AGGAAACTGC ACCATCCAGC 
CAAGGAATAT CCTTCTTTGA AGCACCTCCG ACATCGGACA TTGCTCTCTT CACACCGCCA 
CCCGTCCAGG AAACAGCAGC AGCACCAGTC GAAACACAAG GAATATCTTT CTTTGCACCG 
CAGCCAGATC TCGCACCGAA AGCAGCCCAA GAGATTCCAC TGTTTGTCCC GGCAGCAGTT 
GAGGCACCTC CAGAAGGACA TCCCTTGTAT CCACCGACAT CTACAGCAGT CGCAGAGGAC 
ATTCCTGCCG CTGAGAGCCC ATTATTTGTG CCAGTGCCAA CATCGGCAGC AGATCAGAGT 
CAGGGAGTAC CGCTCTATCC ACCAGAAACA GCCACATCTA ACGCTTCATC TAGCGTTCTC 
TTTGCTGCAT CGGAGCATCA ACCGAGTGCA CCATTATTCG AAGCTGTTCA ACCACCAACC 
ACTGAGGCAG TCGATGCTTC AGCAAGCGAT TTCTTTGGAT CTGCCAATGC TGAAGCTGTC 
CCTGCATCTG CAAATACCGA AACGGCTTAC AGCCCATTCG GACAGACGGT GACCACACCC 
TCGCAAGTGC TGCAGACACC ACCGGCACAA GATTTACTGC CACCGCCTAT TGGATTTGTT 
GCGCCACAAC AACCAGAGGC AGAGCCAGAG CCAGAGGTGG CCGCCGCCGC TCCTCCAGCA 
GAATTCGGAG GAGTTACCGA AGCACTAGCC CTAGCCCACC CACCACTTGC ACCATCGCCG 
CTTCAGAGCT TCTTTTCGCA GCCAGCGACT GCTGCCCCAG AGGATTTCAA CTTTTTTGCC 
GCACCAGGCA GCAGCCTGTT TCCAGAACTG CCACAGCAGC GGGGCATAGC GCCGCCCCCT 
TTGGCACAGG AGCCACCGCC GGCAGCAGCA GAATCTGGTA TTGCACCAGG ACTGGGCTTT 
GATCAATTTC TAAGCCAATC CCAGCAAGCG CAAAGCCACA ACTACATTGG AAATTCAAAT 
GAGAATTCCT ATCAACAAAA TTCAAGTGTC AATACATTCT CCGGCTATTT TGGTGGCCCC 
ACTGTGACAG AAGCCCCAGC CCCACCCCCA GCCACAACCA CAGCAGCAGC AGTGCCACTT 
GCTGCTCCAG TTAGTAACTG TGACCCCTCC CATTTCTTTG ATCAAGTGCC ACAGCCACAG 
GCCCCGACAA ACGCTAACGA GGATCAACGC ATACAGAACT TCTTCAACAA CCCGCCACTG 
CAGGATCAGC CAGCGGCACC GGGCGAACTC AAATACGATA TAGTGCACAG CGGTCTGCCC 
GTCAAACAGC TACAGGCACG CTCCCAGACA CCCATCTCCA ATTTGGTGGA GCCCCCATCC 
TCGTCTGCCT GCTCAGAGTT TTCGACACTG GCACCAGCAG CACCCACAGC TCCAGCAACA 
GCTCCGGCAA CCAATCCAAC AGTCGAGCGT CAAACGGGCG AGGATCTACT GCAGTTGCAT 
CAAGCGGAAT TGCCGGAGGA GATCTTGAAA GAGCTCAGAA TGGCCGCAAA TGCAACGACA 
AGCGACAAGG CAGGCACACC CCCTGTTTCT ATAGTTTATA CGCCTGCTGT GGTCCATTGG 
TTCTACAAAC GTAGAGTGGA CGCGAAACAT ATTTGGACAC CATTCAGTCA CTATGATTCA 
GCGCTGCTTG AGACCAGCCT CCACAATGAT TCCACACTAA TCGTGCCCGT CGAGGGTGGC 
CGCTATGATG TCAGCATCAA GGAACGCACC AAAACCCCCG TCTATTGGGA GGGCAAGGGC 
ATTGAGGTGC GCCGCTGCTC CTGGTTCTAC AAGGGCGTAG ACTCCAAGTA TGTGCCCTAT 
TCTGAGGACA CGGCCGCACT GCTGGAAGAC GAGTACAAAC GTTCGGCAGA GACTGGCGAA 
TGGCATCAGA AGATCATGCT GGCCAACGGT GAACAGGTGG TCTTCCATGG GCCCACAGTC 
ATCGTGCATT TCCTGCCACA ACAGAATTCG GATTCGTGGG GGGCCAGCGC ACAGAGCTCC 
ATGCGTCCGC GGGTGGTCAA GAGGGATCTG GATGACTTTG TCATCAAGCA GGGCGAGTCC 
CAGCGCGTGG ATCATCTGTT GTTTATGGTC CATGGCATTG GTTCGGCCTG TGATCTGAAA 
ATGCGTTCAG TGGAGGAAGT GGTGGATGAT TTCAGAGACA TTGCCCAGCA GCTGGTTCAG 
TCGCACTACA AGAACTCCAC AGATATGGGC CTGGTAGGTC GCGTCGAAGT GTTGCCCATC 
TCCTGGCATG GCCATCTACA CTCCGAGGAG GAGGGCATCG ATGAGAAGCT AAAATCCATT 
ACCCTAGAGT CGATACCACG GCTACGTAAC TTTACGAACG ACACTCTGCT GGACGTACTC 
TTCTACACCA GCCCCAAGTA TTGTCAGAAG ATTATGAACA CGGTGGCCGA TGCTCTCAAT 
GAGGTCTATC TGAAGTACCG ATTCAGGCAT CCGGAATTCA ACGGCGGCGT CTCATTGGCC 
GGCCACAGTC TGGGGTCACT GATCCTATTC GATCTGCTGT GCCATCAGGA GCCACTGAAA 
GAGAGCGAAG AGGAGAATAA GGAGAACCCA GACCAGCTGC CGCAGAAGAA CGAAAACAAC 
TACAAGAAAG TGCAACTGCC CTCCGGCGAT CTGCTGCCGA AGCAGGTCAG CTACACCATG 
GGTCCGGCCG GCACTGGACA GCCGGTCATC AACTACACAC AGCTCATCTT CCACCCGAAG 
AAGTTCTTTG CCCTGGGCTC GCCAATCGGC ATGTTTGTGA CCATTCGGGG CATCGATCAG 
CTTGGCCTGG ACTTTCACCT GCCCACATGT CCGGGTTTCT ACAATATATT CCATCCCTTC 
GATCCGGTGG CCTATCGGAT TGAGGCTCTC GTCAATGCGG ATATGAATGG TATACGTCCT 
GTTCTCATAC CCCATCACAA GGGACGCAAG CGCATGCATC TGGAGCTGAG GGAGACTATG 
ACGCGTGTGG GGGCAGATAT CAAGCAGAGA TTCATGGACA CATTCAAGAC CACCCTGGAC 
AGTGTGAACT TCTTGGCCAC GGTGACCAAA GTTAAAAAGG AGGCAGAAGA GACGCTCGAA 
AAGCAGGTCT TGAATCAGAC TTCGTCGCAG TTCCAGAAAG AAAACGATGA TCAAGAGGAT 
ATTTCTGTGG CCAGCACATC CACAAAGACA AGAAATCGCA CATATTCCAA CTCGACAACG 
GCCTCAGATC CAGAGTTTGT AGAACTGGAC TTTCCGCTAG GCAAACTGAA CGATTCGAAG 
CGCGTCGACT ATGTGCTGCA AGAGGCACCG CTGGAGTTTA TCAATGAGTA CATCTTTGCC 
TTGAGCAGTC ATGTCTGCTA CTGGGGCTCT GAAGACACAA TCCTGTTTGT GATGAAGGAA 
ATCTATGCCA GCCTCGGGAT CAGCACCGAC AGCCAGGTGC CCCAGCAGTC CATGACCATC 
GAGAGGCCCG GCTCATTTGA CAGTAGTCAG TCGCTGTTGC CGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA21159 ( XM_015180407.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015035893.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA21159), partial mRNA.

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CloneID	ODe17805
Clone ID Related Accession (Same CDS sequence)	XM_015180407.1
Accession Version	XM_015180407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589964). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGAAATCAA CTCAAATCAA ATCGAAGGAG GAAACTGCGA CTAATATGAT CGGGAAATAC 
GCAAACATTT CTGAGCCAAA AACTTTGGTG ACCGTGGGAC AGCCATCGAG CATCGATGAC 
AGCGAAGCCC TCGACGAGAT CAACTTGAAT ACGAGCAGCG AGGACTCGAG TGGAGTCAGT 
GGTGGATCGT CGACTGCGTA TCTGCGTGGC ACAGGCGATG GTAATGCCAA TCCCTTGCTA 
GAACCACCAC CGGGCGCTGG AAACGATTCG TTGTTGAGCC AGGCGGCCTC ATCCTTCACA 
GCACTGCCCT CGGTGGCCTC CAATGTGTTT TCCACCTTCT CCAAGAGAAT CTACGCGGGC 
ACAAAGGAAT CGGAAGAGCC TAAATTGGAC ATCCAGCCCA CGTACATACA GCAGGCGGGA 
CACTACGCCC CACCGCCAGT AGCTGCAGCA CCACCACCAC CATTCTACGC GGCACCACCG 
GCGGCAGCGA ATGAGATTGT TGCTGCCCCA GAACCACCCA AGTTCTATGC CCCCACGGAG 
GTGCCAGCCC CGAACGTAGG CATCGCCAGT GCACCACCAC CCACCGCATC GGGAAATCCC 
AACACCTATC GATTCACCGC CAGAAAGAAG CTATACGCAC CGATTCCTGG CTTCACCGAG 
CAGTCGGCAC AGGGTCCGCA GATTCCACAG GCCCCACAGG CAGGTCTGCC CTTCCAGACG 
CCACCATATC CAGCCCAACA GGCAGCTCCA TTTGAGATAT CATCAGCATC TCCTTTTGAG 
ATTCAAGCAG CTCCCCCAGC AGCAGCAGCG ACTGCCACAG AGGAGCGCAA ATCCGGCGGA 
GGACTCTTTT CTCTGAGCAA TCTTGTGCCC ACTGGCGTGC TGCAAAACAT CTCCGGACTT 
GTACAGTCCG CCACAGGCAG GAGCAGCGAG CAGTCCTCTA GTAATCCTCC ACCATCTGGC 
GGGGGCTACT TTGACATCAA CACAGTCGCT CCAGCAAATC CGAGCAACTA TTTCGGTGGC 
CCACCAGCGG CGGCACCGCC AGCGGGAAGC TACTTTGTGC CTGGCGCAGT TCCTGAGGCA 
GCGACAGCAA TTCCTCAACC ACCAGTAGGT GGGTTCTTTC CACAGCCAGC CAATCCGATA 
CCGACAGCTG CTCCCACTTC AGTCGCGGCT CCTCCCACAG TTGGAGGATT CTTTGGGGCA 
ATACCACCTG CTAATCCGCC AGCAACTGAT ATCAATCCCA TCTCGGGAGC ACCACCAGTT 
GGAGGATTCT TTACACCAGG TGTAGCAGCA GTTCCTCCCA CAGTTGGAGG ATTTTATACG 
CCCGTTTCGG CACCGATTGA GATCAATCCT ATTGCTGAAG CACCACCACC GGCAGAAGCA 
GCTATTCCCC CATCATCTGC ACTTGCAGGA GGATTTACTG CAACCCCAGG AGCAGTGCCA 
TCTGTGACTA CATCCACGAC AGCAGCTCCT CCGCCAATTG GAGGGTTCTT TGATCCTAAT 
CCAGCAGCAT CTGGTATTCC TCCCACTTCC ACAGTTGGAG GATTCTTTAC GCCAGCAGCA 
GTTCCACCTG TGGCCACAGC AGCACCTGTT CCACCTGTTG CCACAGTAAC AGCAGCAGCT 
CCTCCACCAG TAGGAGGATT CTTTGCACCC GCAGGAGCAG GGCCACCATC CAACGCAACT 
CCACTGGCAG GACCACCTCT GGGGACAGCA CCTCCATTGG CAGGACCACC TTTAGGATCA 
GCAGCAACTG TCGCTGGACC ACCACCTGCC GCTGGACAGG CATCGTATCG CCTCCAGAAG 
GGCACACGTC TCTACAAGAG TCCATTGACG GCACAGGAAA CTGCCACAGC GTCTGCTTTC 
GGCACTCCCT TTGCCACAAC AGCAGCTGTC CCAGCTGCCG TGTTCAATCC ATTTGGAACT 
GGAGCTTCAG GAGTATTTAA TCCATTAGCT GGCAACCCAC CAGTTGGTCA GCAGGAGAAC 
GAAGATAACT CAGCCGTTGC ACTATTTGCA CCTCCTTCTG CACCAGTGGC AGGACAGACC 
TTAGATCAAG GACCGGCTCC TCCAGCTGCC TTTGCATCGA CAAATCAGGG AAGTCCCTTC 
CCCGCTCCAC AGCCAGGACC CCAGGCAACG CCATTCTTCA TTCCACAAAC GGGGGACAGA 
CCTCCAGTAC CCACCAGCCA GGAAACTGCA CCATCCAGCC AAGGAATATC CTTCTTTGAA 
GCACCTCCGA CATCGGACAT TGCTCTCTTC ACACCGCCAC CCGTCCAGGA AACAGCAGCA 
GCACCAGTCG AAACACAAGG AATATCTTTC TTTGCACCGC AGCCAGATCT CGCACCGAAA 
GCAGCCCAAG AGATTCCACT GTTTGTCCCG GCAGCAGTTG AGGCACCTCC AGAAGGACAT 
CCCTTGTATC CACCGACATC TACAGCAGTC GCAGAGGACA TTCCTGCCGC TGAGAGCCCA 
TTATTTGTGC CAGTGCCAAC ATCGGCAGCA GATCAGAGTC AGGGAGTACC GCTCTATCCA 
CCAGAAACAG CCACATCTAA CGCTTCATCT AGCGTTCTCT TTGCTGCATC GGAGCATCAA 
CCGAGTGCAC CATTATTCGA AGCTGTTCAA CCACCAACCA CTGAGGCAGT CGATGCTTCA 
GCAAGCGATT TCTTTGGATC TGCCAATGCT GAAGCTGTCC CTGCATCTGC AAATACCGAA 
ACGGCTTACA GCCCATTCGG ACAGACGGTG ACCACACCCT CGCAAGTGCT GCAGACACCA 
CCGGCACAAG ATTTACTGCC ACCGCCTATT GGATTTGTTG CGCCACAACA ACCAGAGGCA 
GAGCCAGAGC CAGAGGTGGC CGCCGCCGCT CCTCCAGCAG AATTCGGAGG AGTTACCGAA 
GCACTAGCCC TAGCCCACCC ACCACTTGCA CCATCGCCGC TTCAGAGCTT CTTTTCGCAG 
CCAGCGACTG CTGCCCCAGA GGATTTCAAC TTTTTTGCCG CACCAGGCAG CAGCCTGTTT 
CCAGAACTGC CACAGCAGCG GGGCATAGCG CCGCCCCCTT TGGCACAGGA GCCACCGCCG 
GCAGCAGCAG AATCTGGTAT TGCACCAGGA CTGGGCTTTG ATCAATTTCT AAGCCAATCC 
CAGCAAGCGC AAAGCCACAA CTACATTGGA AATTCAAATG AGAATTCCTA TCAACAAAAT 
TCAAGTGTCA ATACATTCTC CGGCTATTTT GGTGGCCCCA CTGTGACAGA AGCCCCAGCC 
CCACCCCCAG CCACAACCAC AGCAGCAGCA GTGCCACTTG CTGCTCCAGT TAGTAACTGT 
GACCCCTCCC ATTTCTTTGA TCAAGTGCCA CAGCCACAGG CCCCGACAAA CGCTAACGAG 
GATCAACGCA TACAGAACTT CTTCAACAAC CCGCCACTGC AGGATCAGCC AGCGGCACCG 
GGCGAACTCA AATACGATAT AGTGCACAGC GGTCTGCCCG TCAAACAGCT ACAGGCACGC 
TCCCAGACAC CCATCTCCAA TTTGGTGGAG CCCCCATCCT CGTCTGCCTG CTCAGAGTTT 
TCGACACTGG CACCAGCAGC ACCCACAGCT CCAGCAACAG CTCCGGCAAC CAATCCAACA 
GTCGAGCGTC AAACGGGCGA GGATCTACTG CAGTTGCATC AAGCGGAATT GCCGGAGGAG 
ATCTTGAAAG AGCTCAGAAT GGCCGCAAAT GCAACGACAA GCGACAAGGC AGGCACACCC 
CCTGTTTCTA TAGTTTATAC GCCTGCTGTG GTCCATTGGT TCTACAAACG TAGAGTGGAC 
GCGAAACATA TTTGGACACC ATTCAGTCAC TATGATTCAG CGCTGCTTGA GACCAGCCTC 
CACAATGAAG ATTCCACACT AATCGTGCCC GTCGAGGGTG GCCGCTATGA TGTCAGCATC 
AAGGAACGCA CCAAAACCCC CGTCTATTGG GAGGGCAAGG GCATTGAGGT GCGCCGCTGC 
TCCTGGTTCT ACAAGGGCGT AGACTCCAAG TATGTGCCCT ATTCTGAGGA CACGGCCGCA 
CTGCTGGAAG ACGAGTACAA ACGTTCGGCA GAGACTGGCG AATGGCATCA GAAGATCATG 
CTGGCCAACG GTGAACAGGT GGTCTTCCAT GGGCCCACAG TCATCGTGCA TTTCCTGCCA 
CAACAGAATT CGGATTCGTG GGGGGCCAGC GCACAGAGCT CCATGCGTCC GCGGGTGGTC 
AAGAGGGATC TGGATGACTT TGTCATCAAG CAGGGCGAGT CCCAGCGCGT GGATCATCTG 
TTGTTTATGG TCCATGGCAT TGGTTCGGCC TGTGATCTGA AAATGCGTTC AGTGGAGGAA 
GTGGTGGATG ATTTCAGAGA CATTGCCCAG CAGCTGGTTC AGTCGCACTA CAAGAACTCC 
ACAGATATGG GCCTGGTAGG TCGCGTCGAA GTGTTGCCCA TCTCCTGGCA TGGCCATCTA 
CACTCCGAGG AGGAGGGCAT CGATGAGAAG CTAAAATCCA TTACCCTAGA GTCGATACCA 
CGGCTACGTA ACTTTACGAA CGACACTCTG CTGGACGTAC TCTTCTACAC CAGCCCCAAG 
TATTGTCAGA AGATTATGAA CACGGTGGCC GATGCTCTCA ATGAGGTCTA TCTGAAGTAC 
CGATTCAGGC ATCCGGAATT CAACGGCGGC GTCTCATTGG CCGGCCACAG TCTGGGGTCA 
CTGATCCTAT TCGATCTGCT GTGCCATCAG GAGCCACTGA AAGAGAGCGA AGAGGAGAAT 
AAGGAGAACC CAGACCAGCT GCCGCAGAAG AACGAAAACA ACTACAAGAA AGTGCAACTG 
CCCTCCGGCG ATCTGCTGCC GAAGCAGGTC AGCTACACCA TGGGTCCGGC CGGCACTGGA 
CAGCCGGTCA TCAACTACAC ACAGCTCATC TTCCACCCGA AGAAGTTCTT TGCCCTGGGC 
TCGCCAATCG GCATGTTTGT GACCATTCGG GGCATCGATC AGCTTGGCCT GGACTTTCAC 
CTGCCCACAT GTCCGGGTTT CTACAATATA TTCCATCCCT TCGATCCGGT GGCCTATCGG 
ATTGAGGCTC TCGTCAATGC GGATATGAAT GGTATACGTC CTGTTCTCAT ACCCCATCAC 
AAGGGACGCA AGCGCATGCA TCTGGAGCTG AGGGAGACTA TGACGCGTGT GGGGGCAGAT 
ATCAAGCAGA GATTCATGGA CACATTCAAG ACCACCCTGG ACAGTGTGAA CTTCTTGGCC 
ACGGTGACCA AAGTTAAAAA GGAGGCAGAA GAGACGCTCG AAAAGCAGGT CTTGAATCAG 
ACTTCGTCGC AGTTCCAGAA AGAAAACGAT GATCAAGAGG ATATTTCTGT GGCCAGCACA 
TCCACAAAGA CAAGAAATCG CACATATTCC AACTCGACAA CGGCCTCAGA TCCAGAGTTT 
GTAGAACTGG ACTTTCCGCT AGGCAAACTG AACGATTCGA AGCGCGTCGA CTATGTGCTG 
CAAGAGGCAC CGCTGGAGTT TATCAATGAG TACATCTTTG CCTTGAGCAG TCATGTCTGC 
TACTGGGGCT CTGAAGACAC AATCCTGTTT GTGATGAAGG AAATCTATGC CAGCCTCGGG 
ATCAGCACCG ACAGCCAGGT GCCCCAGCAG TCCATGACCA TCGAGAGGCC CGGCTCATTT 
GACAGTAGTC AGTCGCTGTT GCCGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015035893.1
CDS	1..5670
Translation	MKSTQIKSKEETATNMIGKYANISEPKTLVTVGQPSSIDDSEALDEINLNTSSEDSSGVSGGSSTAYLRGTGDGNANPLLEPPPGAGNDSLLSQAASSFTALPSVASNVFSTFSKRIYAGTKESEEPKLDIQPTYIQQAGHYAPPPVAAAPPPPFYAAPPAAANEIVAAPEPPKFYAPTEVPAPNVGIASAPPPTASGNPNTYRFTARKKLYAPIPGFTEQSAQGPQIPQAPQAGLPFQTPPYPAQQAAPFEISSASPFEIQAAPPAAAATATEERKSGGGLFSLSNLVPTGVLQNISGLVQSATGRSSEQSSSNPPPSGGGYFDINTVAPANPSNYFGGPPAAAPPAGSYFVPGAVPEAATAIPQPPVGGFFPQPANPIPTAAPTSVAAPPTVGGFFGAIPPANPPATDINPISGAPPVGGFFTPGVAAVPPTVGGFYTPVSAPIEINPIAEAPPPAEAAIPPSSALAGGFTATPGAVPSVTTSTTAAPPPIGGFFDPNPAASGIPPTSTVGGFFTPAAVPPVATAAPVPPVATVTAAAPPPVGGFFAPAGAGPPSNATPLAGPPLGTAPPLAGPPLGSAATVAGPPPAAGQASYRLQKGTRLYKSPLTAQETATASAFGTPFATTAAVPAAVFNPFGTGASGVFNPLAGNPPVGQQENEDNSAVALFAPPSAPVAGQTLDQGPAPPAAFASTNQGSPFPAPQPGPQATPFFIPQTGDRPPVPTSQETAPSSQGISFFEAPPTSDIALFTPPPVQETAAAPVETQGISFFAPQPDLAPKAAQEIPLFVPAAVEAPPEGHPLYPPTSTAVAEDIPAAESPLFVPVPTSAADQSQGVPLYPPETATSNASSSVLFAASEHQPSAPLFEAVQPPTTEAVDASASDFFGSANAEAVPASANTETAYSPFGQTVTTPSQVLQTPPAQDLLPPPIGFVAPQQPEAEPEPEVAAAAPPAEFGGVTEALALAHPPLAPSPLQSFFSQPATAAPEDFNFFAAPGSSLFPELPQQRGIAPPPLAQEPPPAAAESGIAPGLGFDQFLSQSQQAQSHNYIGNSNENSYQQNSSVNTFSGYFGGPTVTEAPAPPPATTTAAAVPLAAPVSNCDPSHFFDQVPQPQAPTNANEDQRIQNFFNNPPLQDQPAAPGELKYDIVHSGLPVKQLQARSQTPISNLVEPPSSSACSEFSTLAPAAPTAPATAPATNPTVERQTGEDLLQLHQAELPEEILKELRMAANATTSDKAGTPPVSIVYTPAVVHWFYKRRVDAKHIWTPFSHYDSALLETSLHNEDSTLIVPVEGGRYDVSIKERTKTPVYWEGKGIEVRRCSWFYKGVDSKYVPYSEDTAALLEDEYKRSAETGEWHQKIMLANGEQVVFHGPTVIVHFLPQQNSDSWGASAQSSMRPRVVKRDLDDFVIKQGESQRVDHLLFMVHGIGSACDLKMRSVEEVVDDFRDIAQQLVQSHYKNSTDMGLVGRVEVLPISWHGHLHSEEEGIDEKLKSITLESIPRLRNFTNDTLLDVLFYTSPKYCQKIMNTVADALNEVYLKYRFRHPEFNGGVSLAGHSLGSLILFDLLCHQEPLKESEEENKENPDQLPQKNENNYKKVQLPSGDLLPKQVSYTMGPAGTGQPVINYTQLIFHPKKFFALGSPIGMFVTIRGIDQLGLDFHLPTCPGFYNIFHPFDPVAYRIEALVNADMNGIRPVLIPHHKGRKRMHLELRETMTRVGADIKQRFMDTFKTTLDSVNFLATVTKVKKEAEETLEKQVLNQTSSQFQKENDDQEDISVASTSTKTRNRTYSNSTTASDPEFVELDFPLGKLNDSKRVDYVLQEAPLEFINEYIFALSSHVCYWGSEDTILFVMKEIYASLGISTDSQVPQQSMTIERPGSFDSSQSLLPM

Target ORF information:

RefSeq Version	XM_015180407.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA21159), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015180407.1

ORF Insert Sequence:

ATGAAATCAA CTCAAATCAA ATCGAAGGAG GAAACTGCGA CTAATATGAT CGGGAAATAC 
GCAAACATTT CTGAGCCAAA AACTTTGGTG ACCGTGGGAC AGCCATCGAG CATCGATGAC 
AGCGAAGCCC TCGACGAGAT CAACTTGAAT ACGAGCAGCG AGGACTCGAG TGGAGTCAGT 
GGTGGATCGT CGACTGCGTA TCTGCGTGGC ACAGGCGATG GTAATGCCAA TCCCTTGCTA 
GAACCACCAC CGGGCGCTGG AAACGATTCG TTGTTGAGCC AGGCGGCCTC ATCCTTCACA 
GCACTGCCCT CGGTGGCCTC CAATGTGTTT TCCACCTTCT CCAAGAGAAT CTACGCGGGC 
ACAAAGGAAT CGGAAGAGCC TAAATTGGAC ATCCAGCCCA CGTACATACA GCAGGCGGGA 
CACTACGCCC CACCGCCAGT AGCTGCAGCA CCACCACCAC CATTCTACGC GGCACCACCG 
GCGGCAGCGA ATGAGATTGT TGCTGCCCCA GAACCACCCA AGTTCTATGC CCCCACGGAG 
GTGCCAGCCC CGAACGTAGG CATCGCCAGT GCACCACCAC CCACCGCATC GGGAAATCCC 
AACACCTATC GATTCACCGC CAGAAAGAAG CTATACGCAC CGATTCCTGG CTTCACCGAG 
CAGTCGGCAC AGGGTCCGCA GATTCCACAG GCCCCACAGG CAGGTCTGCC CTTCCAGACG 
CCACCATATC CAGCCCAACA GGCAGCTCCA TTTGAGATAT CATCAGCATC TCCTTTTGAG 
ATTCAAGCAG CTCCCCCAGC AGCAGCAGCG ACTGCCACAG AGGAGCGCAA ATCCGGCGGA 
GGACTCTTTT CTCTGAGCAA TCTTGTGCCC ACTGGCGTGC TGCAAAACAT CTCCGGACTT 
GTACAGTCCG CCACAGGCAG GAGCAGCGAG CAGTCCTCTA GTAATCCTCC ACCATCTGGC 
GGGGGCTACT TTGACATCAA CACAGTCGCT CCAGCAAATC CGAGCAACTA TTTCGGTGGC 
CCACCAGCGG CGGCACCGCC AGCGGGAAGC TACTTTGTGC CTGGCGCAGT TCCTGAGGCA 
GCGACAGCAA TTCCTCAACC ACCAGTAGGT GGGTTCTTTC CACAGCCAGC CAATCCGATA 
CCGACAGCTG CTCCCACTTC AGTCGCGGCT CCTCCCACAG TTGGAGGATT CTTTGGGGCA 
ATACCACCTG CTAATCCGCC AGCAACTGAT ATCAATCCCA TCTCGGGAGC ACCACCAGTT 
GGAGGATTCT TTACACCAGG TGTAGCAGCA GTTCCTCCCA CAGTTGGAGG ATTTTATACG 
CCCGTTTCGG CACCGATTGA GATCAATCCT ATTGCTGAAG CACCACCACC GGCAGAAGCA 
GCTATTCCCC CATCATCTGC ACTTGCAGGA GGATTTACTG CAACCCCAGG AGCAGTGCCA 
TCTGTGACTA CATCCACGAC AGCAGCTCCT CCGCCAATTG GAGGGTTCTT TGATCCTAAT 
CCAGCAGCAT CTGGTATTCC TCCCACTTCC ACAGTTGGAG GATTCTTTAC GCCAGCAGCA 
GTTCCACCTG TGGCCACAGC AGCACCTGTT CCACCTGTTG CCACAGTAAC AGCAGCAGCT 
CCTCCACCAG TAGGAGGATT CTTTGCACCC GCAGGAGCAG GGCCACCATC CAACGCAACT 
CCACTGGCAG GACCACCTCT GGGGACAGCA CCTCCATTGG CAGGACCACC TTTAGGATCA 
GCAGCAACTG TCGCTGGACC ACCACCTGCC GCTGGACAGG CATCGTATCG CCTCCAGAAG 
GGCACACGTC TCTACAAGAG TCCATTGACG GCACAGGAAA CTGCCACAGC GTCTGCTTTC 
GGCACTCCCT TTGCCACAAC AGCAGCTGTC CCAGCTGCCG TGTTCAATCC ATTTGGAACT 
GGAGCTTCAG GAGTATTTAA TCCATTAGCT GGCAACCCAC CAGTTGGTCA GCAGGAGAAC 
GAAGATAACT CAGCCGTTGC ACTATTTGCA CCTCCTTCTG CACCAGTGGC AGGACAGACC 
TTAGATCAAG GACCGGCTCC TCCAGCTGCC TTTGCATCGA CAAATCAGGG AAGTCCCTTC 
CCCGCTCCAC AGCCAGGACC CCAGGCAACG CCATTCTTCA TTCCACAAAC GGGGGACAGA 
CCTCCAGTAC CCACCAGCCA GGAAACTGCA CCATCCAGCC AAGGAATATC CTTCTTTGAA 
GCACCTCCGA CATCGGACAT TGCTCTCTTC ACACCGCCAC CCGTCCAGGA AACAGCAGCA 
GCACCAGTCG AAACACAAGG AATATCTTTC TTTGCACCGC AGCCAGATCT CGCACCGAAA 
GCAGCCCAAG AGATTCCACT GTTTGTCCCG GCAGCAGTTG AGGCACCTCC AGAAGGACAT 
CCCTTGTATC CACCGACATC TACAGCAGTC GCAGAGGACA TTCCTGCCGC TGAGAGCCCA 
TTATTTGTGC CAGTGCCAAC ATCGGCAGCA GATCAGAGTC AGGGAGTACC GCTCTATCCA 
CCAGAAACAG CCACATCTAA CGCTTCATCT AGCGTTCTCT TTGCTGCATC GGAGCATCAA 
CCGAGTGCAC CATTATTCGA AGCTGTTCAA CCACCAACCA CTGAGGCAGT CGATGCTTCA 
GCAAGCGATT TCTTTGGATC TGCCAATGCT GAAGCTGTCC CTGCATCTGC AAATACCGAA 
ACGGCTTACA GCCCATTCGG ACAGACGGTG ACCACACCCT CGCAAGTGCT GCAGACACCA 
CCGGCACAAG ATTTACTGCC ACCGCCTATT GGATTTGTTG CGCCACAACA ACCAGAGGCA 
GAGCCAGAGC CAGAGGTGGC CGCCGCCGCT CCTCCAGCAG AATTCGGAGG AGTTACCGAA 
GCACTAGCCC TAGCCCACCC ACCACTTGCA CCATCGCCGC TTCAGAGCTT CTTTTCGCAG 
CCAGCGACTG CTGCCCCAGA GGATTTCAAC TTTTTTGCCG CACCAGGCAG CAGCCTGTTT 
CCAGAACTGC CACAGCAGCG GGGCATAGCG CCGCCCCCTT TGGCACAGGA GCCACCGCCG 
GCAGCAGCAG AATCTGGTAT TGCACCAGGA CTGGGCTTTG ATCAATTTCT AAGCCAATCC 
CAGCAAGCGC AAAGCCACAA CTACATTGGA AATTCAAATG AGAATTCCTA TCAACAAAAT 
TCAAGTGTCA ATACATTCTC CGGCTATTTT GGTGGCCCCA CTGTGACAGA AGCCCCAGCC 
CCACCCCCAG CCACAACCAC AGCAGCAGCA GTGCCACTTG CTGCTCCAGT TAGTAACTGT 
GACCCCTCCC ATTTCTTTGA TCAAGTGCCA CAGCCACAGG CCCCGACAAA CGCTAACGAG 
GATCAACGCA TACAGAACTT CTTCAACAAC CCGCCACTGC AGGATCAGCC AGCGGCACCG 
GGCGAACTCA AATACGATAT AGTGCACAGC GGTCTGCCCG TCAAACAGCT ACAGGCACGC 
TCCCAGACAC CCATCTCCAA TTTGGTGGAG CCCCCATCCT CGTCTGCCTG CTCAGAGTTT 
TCGACACTGG CACCAGCAGC ACCCACAGCT CCAGCAACAG CTCCGGCAAC CAATCCAACA 
GTCGAGCGTC AAACGGGCGA GGATCTACTG CAGTTGCATC AAGCGGAATT GCCGGAGGAG 
ATCTTGAAAG AGCTCAGAAT GGCCGCAAAT GCAACGACAA GCGACAAGGC AGGCACACCC 
CCTGTTTCTA TAGTTTATAC GCCTGCTGTG GTCCATTGGT TCTACAAACG TAGAGTGGAC 
GCGAAACATA TTTGGACACC ATTCAGTCAC TATGATTCAG CGCTGCTTGA GACCAGCCTC 
CACAATGAAG ATTCCACACT AATCGTGCCC GTCGAGGGTG GCCGCTATGA TGTCAGCATC 
AAGGAACGCA CCAAAACCCC CGTCTATTGG GAGGGCAAGG GCATTGAGGT GCGCCGCTGC 
TCCTGGTTCT ACAAGGGCGT AGACTCCAAG TATGTGCCCT ATTCTGAGGA CACGGCCGCA 
CTGCTGGAAG ACGAGTACAA ACGTTCGGCA GAGACTGGCG AATGGCATCA GAAGATCATG 
CTGGCCAACG GTGAACAGGT GGTCTTCCAT GGGCCCACAG TCATCGTGCA TTTCCTGCCA 
CAACAGAATT CGGATTCGTG GGGGGCCAGC GCACAGAGCT CCATGCGTCC GCGGGTGGTC 
AAGAGGGATC TGGATGACTT TGTCATCAAG CAGGGCGAGT CCCAGCGCGT GGATCATCTG 
TTGTTTATGG TCCATGGCAT TGGTTCGGCC TGTGATCTGA AAATGCGTTC AGTGGAGGAA 
GTGGTGGATG ATTTCAGAGA CATTGCCCAG CAGCTGGTTC AGTCGCACTA CAAGAACTCC 
ACAGATATGG GCCTGGTAGG TCGCGTCGAA GTGTTGCCCA TCTCCTGGCA TGGCCATCTA 
CACTCCGAGG AGGAGGGCAT CGATGAGAAG CTAAAATCCA TTACCCTAGA GTCGATACCA 
CGGCTACGTA ACTTTACGAA CGACACTCTG CTGGACGTAC TCTTCTACAC CAGCCCCAAG 
TATTGTCAGA AGATTATGAA CACGGTGGCC GATGCTCTCA ATGAGGTCTA TCTGAAGTAC 
CGATTCAGGC ATCCGGAATT CAACGGCGGC GTCTCATTGG CCGGCCACAG TCTGGGGTCA 
CTGATCCTAT TCGATCTGCT GTGCCATCAG GAGCCACTGA AAGAGAGCGA AGAGGAGAAT 
AAGGAGAACC CAGACCAGCT GCCGCAGAAG AACGAAAACA ACTACAAGAA AGTGCAACTG 
CCCTCCGGCG ATCTGCTGCC GAAGCAGGTC AGCTACACCA TGGGTCCGGC CGGCACTGGA 
CAGCCGGTCA TCAACTACAC ACAGCTCATC TTCCACCCGA AGAAGTTCTT TGCCCTGGGC 
TCGCCAATCG GCATGTTTGT GACCATTCGG GGCATCGATC AGCTTGGCCT GGACTTTCAC 
CTGCCCACAT GTCCGGGTTT CTACAATATA TTCCATCCCT TCGATCCGGT GGCCTATCGG 
ATTGAGGCTC TCGTCAATGC GGATATGAAT GGTATACGTC CTGTTCTCAT ACCCCATCAC 
AAGGGACGCA AGCGCATGCA TCTGGAGCTG AGGGAGACTA TGACGCGTGT GGGGGCAGAT 
ATCAAGCAGA GATTCATGGA CACATTCAAG ACCACCCTGG ACAGTGTGAA CTTCTTGGCC 
ACGGTGACCA AAGTTAAAAA GGAGGCAGAA GAGACGCTCG AAAAGCAGGT CTTGAATCAG 
ACTTCGTCGC AGTTCCAGAA AGAAAACGAT GATCAAGAGG ATATTTCTGT GGCCAGCACA 
TCCACAAAGA CAAGAAATCG CACATATTCC AACTCGACAA CGGCCTCAGA TCCAGAGTTT 
GTAGAACTGG ACTTTCCGCT AGGCAAACTG AACGATTCGA AGCGCGTCGA CTATGTGCTG 
CAAGAGGCAC CGCTGGAGTT TATCAATGAG TACATCTTTG CCTTGAGCAG TCATGTCTGC 
TACTGGGGCT CTGAAGACAC AATCCTGTTT GTGATGAAGG AAATCTATGC CAGCCTCGGG 
ATCAGCACCG ACAGCCAGGT GCCCCAGCAG TCCATGACCA TCGAGAGGCC CGGCTCATTT 
GACAGTAGTC AGTCGCTGTT GCCGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA21159 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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