Dpse\GA12272 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA12272
Entrez Gene ID	4800270
Full Name	GA12272 gene product from transcript GA12272-RA
Synonyms	GA12272,dpse_GLEANR_4684
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001357566.3	XP_001357603.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA12272 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA12272 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe18952	XM_001357566.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA12272), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA12272 ( XM_001357566.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001357603.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA12272), mRNA.

Price & Availability↑

CloneID	ODe18952
Clone ID Related Accession (Same CDS sequence)	XM_001357566.3
Accession Version	XM_001357566.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001357566.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTTTGA ACTCTGGAAG GAGGGGGAGG TGTGCTCGAG AGCCCACGGC CCACGCCACT 
TCCACTTTCG TTCGGGTCGC AGAAGTCGCA GTAGAGCAAA AGCGACTTTC TTTGTCAAAC 
CAACGATCGA TCGATCCAAG ACCTTATATG GAAGAGAGGC CCCCTCCCCA CTGCTTCTAC 
CACTCTCTGC TAACCAGTTT GAGGCGCAGT CGGCGACGCG GCCGCTGCCG TTGCTGCTTG 
GTTCTCGACG CGGATTCAAA TGATTCTTGT TTGGCTTTCA TAAAAAGTCT TTGGCCAACT 
TTCTTGAGCT TCATTCGCGC TCCACTCGCG CGATCCGTCA GTTCGTTACG ACCCAAAATG 
AAAGCGGTTC TCTGTGTGCT GCCCCTGTTA GCGGCCTTGG TGTGCTCCCT GCCCACCGCC 
GAGAAGGAGA GCTCCTCCAA GGGCGCCTCC TTCGCCAGTG CGACCTCGCA GCTGATTGCC 
ATGCAGCTGC TGAAGTTCAA CAAAGACATC GACGCCAACC AGGTGCACTC GCCCCTGGGA 
GTGACCTCCA TTCTGGGTGT GCTGGCAGAG GCCTCCGAGG GCGAGACCTA CGCGGAGTTC 
ACCAAGGTCT TCGGATATCC CAAGGATCGC TCCCAGCTGC GGGACGTGTA CCGTCGAGTG 
CTAGGACGCT ACCAGAACCG AGATGCCGCC GTGGCACTGC CCTCGTTCCA GACCTGGTTC 
TACATCTACC GCAACCACAG CGCCCGGGAG GAGTTCAAGG AGCTGCTGCA GCAGCACTAC 
TTCGTGGAGG TGAAGGACAT CAACCGGAAG GACTACGACT GGAACGAGCC CAACACTTCG 
CTCCAGCTCC AGCCAGAGGG AAGCGGATCC GATGCCTCCG AGGCCGGGGA GGAGAGCCTC 
AGCAGCACGG AGCAGAGTAA CAGCAAGGAT GTGATCGGAT TCGAGACCCT GAAGCGCATC 
AACCTGGACG ACGATTTGCT GTCAGTGCCC GCCGACACCT ACGGCGAGGA GGTGCTGGAC 
AAGGAGGCCT CCAAGCTGGA CCGCGACGTG GACGACAAGC AGTATGTGGA AAAACCAGTT 
GCCCAGGCGG AGGCCGAGCA GCTGCAAAGG GAGCAGGCGG CAGCCACCAC CACCACGGAG 
GCGGAAGCGG AGGCCACAAC CCCAGAAGCA GAAGCAGCAG CAGAAGCAGC AGCAGAGGCT 
GTGGAAAAAC AGGAAAAACC AGCCATCGCC GCAGCTGGGG AAAATCCAGT GGAGAAGCAG 
CAGAACAAGA GGAGCGACAA CGAGGAGAAT CAGAGTCAGA ATCTCAATCT GGAGGAGAAC 
GAGACGGTGC AGGAGGAGGA GAAGCTGCGC AAAGTGGTCG GCTCTGAGGC GGTGACCGCC 
GGTGAACCCG AGAAGGTGCG TCTACCCCTC CAGAAACTGG AAAGCGCCGT CAACACAGCG 
GCGTCCAAGG AGGGCGGTGC CGACGAGATA ATGCTGGCCC TGGAGTCGCA TCTGGGAGCG 
GTTAGTCGGG TCTATGGAGC CCGCAGTCTG TTCCGGCAGG ACGACATCAC CTCTGCCCTG 
AGCGCCAACT CCATTACGGG CCGCTCCGCC GGAGCCAAGT CCAAGATGCT GCTCTTCAAC 
GGCCTGTACT ACAGGGGGAG CTGGGCCACG CCCTTCTACC AACTGAGGGA TGGGAGTGAC 
GAGTTCTTCT TCATGACCAA CGAGGACGCC ATGAAGGCTC CGATGATGCA CGCGCGCGGC 
CAGTTCCTGG TGGCCGATCT GCCCCAGCTG AAGGCCCGCG TCCTGTCGCT GCCCTACGAG 
ACCTCGCGCT ACGCCCTGTG CATCCTGCTG CCCGACGAGA CGGAGGGCCT CCACGATGTC 
ATCGCCCAGA TGCAGCCCAG CGACTTCCAG CTGGGACGCG AGAAGGCGCA GCTCCGGCAG 
ATGCACGTGT CCCTGCCCAA GTTCCAGGTG GAGGAGACCT CGCGCTCCGA GTCGATGCTC 
AAGCAGATGG GCCTGCGGCG CCTCTTCTCC CGCACCGAGG CCCAGTTGGG GCTGCTCTCA 
GAGGACCCCG ACGTCCATGT GGATGAGATT GTCCAGTTCG TGAATGTCCG CGTGGACGAG 
GGCGGCAGCA GCGCCAACGC CCTCTCCGCG GCCACCCAGG CCCGCTCTCC ACAGGCTGCA 
GAGGATTCCG CCTCTGTTCT GCCCGTGCCC GAGCCGGAGC CGGAGCCGGG CGTGGAGCGG 
TTCGAGGTGA ACCGCCCCTT CGCCTACTTC ATCATGGACT GCGAGGAGCA GTTCATCCTC 
GCCTCTGGCA AGGTCTACAC GCCAGAGTTC AAGGACGATC TGCCCGGTGT TTCTGTGGAG 
ATTGAGCTGG AACAGTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001357603.3
CDS	71..2431
Translation	MTLNSGRRGRCAREPTAHATSTFVRVAEVAVEQKRLSLSNQRSIDPRPYMEERPPPHCFYHSLLTSLRRSRRRGRCRCCLVLDADSNDSCLAFIKSLWPTFLSFIRAPLARSVSSLRPKMKAVLCVLPLLAALVCSLPTAEKESSSKGASFASATSQLIAMQLLKFNKDIDANQVHSPLGVTSILGVLAEASEGETYAEFTKVFGYPKDRSQLRDVYRRVLGRYQNRDAAVALPSFQTWFYIYRNHSAREEFKELLQQHYFVEVKDINRKDYDWNEPNTSLQLQPEGSGSDASEAGEESLSSTEQSNSKDVIGFETLKRINLDDDLLSVPADTYGEEVLDKEASKLDRDVDDKQYVEKPVAQAEAEQLQREQAAATTTTEAEAEATTPEAEAAAEAAAEAVEKQEKPAIAAAGENPVEKQQNKRSDNEENQSQNLNLEENETVQEEEKLRKVVGSEAVTAGEPEKVRLPLQKLESAVNTAASKEGGADEIMLALESHLGAVSRVYGARSLFRQDDITSALSANSITGRSAGAKSKMLLFNGLYYRGSWATPFYQLRDGSDEFFFMTNEDAMKAPMMHARGQFLVADLPQLKARVLSLPYETSRYALCILLPDETEGLHDVIAQMQPSDFQLGREKAQLRQMHVSLPKFQVEETSRSESMLKQMGLRRLFSRTEAQLGLLSEDPDVHVDEIVQFVNVRVDEGGSSANALSAATQARSPQAAEDSASVLPVPEPEPEPGVERFEVNRPFAYFIMDCEEQFILASGKVYTPEFKDDLPGVSVEIELEQS

Target ORF information:

RefSeq Version	XM_001357566.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA12272), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001357566.3

ORF Insert Sequence:

ATGACTTTGA ACTCTGGAAG GAGGGGGAGG TGTGCTCGAG AGCCCACGGC CCACGCCACT 
TCCACTTTCG TTCGGGTCGC AGAAGTCGCA GTAGAGCAAA AGCGACTTTC TTTGTCAAAC 
CAACGATCGA TCGATCCAAG ACCTTATATG GAAGAGAGGC CCCCTCCCCA CTGCTTCTAC 
CACTCTCTGC TAACCAGTTT GAGGCGCAGT CGGCGACGCG GCCGCTGCCG TTGCTGCTTG 
GTTCTCGACG CGGATTCAAA TGATTCTTGT TTGGCTTTCA TAAAAAGTCT TTGGCCAACT 
TTCTTGAGCT TCATTCGCGC TCCACTCGCG CGATCCGTCA GTTCGTTACG ACCCAAAATG 
AAAGCGGTTC TCTGTGTGCT GCCCCTGTTA GCGGCCTTGG TGTGCTCCCT GCCCACCGCC 
GAGAAGGAGA GCTCCTCCAA GGGCGCCTCC TTCGCCAGTG CGACCTCGCA GCTGATTGCC 
ATGCAGCTGC TGAAGTTCAA CAAAGACATC GACGCCAACC AGGTGCACTC GCCCCTGGGA 
GTGACCTCCA TTCTGGGTGT GCTGGCAGAG GCCTCCGAGG GCGAGACCTA CGCGGAGTTC 
ACCAAGGTCT TCGGATATCC CAAGGATCGC TCCCAGCTGC GGGACGTGTA CCGTCGAGTG 
CTAGGACGCT ACCAGAACCG AGATGCCGCC GTGGCACTGC CCTCGTTCCA GACCTGGTTC 
TACATCTACC GCAACCACAG CGCCCGGGAG GAGTTCAAGG AGCTGCTGCA GCAGCACTAC 
TTCGTGGAGG TGAAGGACAT CAACCGGAAG GACTACGACT GGAACGAGCC CAACACTTCG 
CTCCAGCTCC AGCCAGAGGG AAGCGGATCC GATGCCTCCG AGGCCGGGGA GGAGAGCCTC 
AGCAGCACGG AGCAGAGTAA CAGCAAGGAT GTGATCGGAT TCGAGACCCT GAAGCGCATC 
AACCTGGACG ACGATTTGCT GTCAGTGCCC GCCGACACCT ACGGCGAGGA GGTGCTGGAC 
AAGGAGGCCT CCAAGCTGGA CCGCGACGTG GACGACAAGC AGTATGTGGA AAAACCAGTT 
GCCCAGGCGG AGGCCGAGCA GCTGCAAAGG GAGCAGGCGG CAGCCACCAC CACCACGGAG 
GCGGAAGCGG AGGCCACAAC CCCAGAAGCA GAAGCAGCAG CAGAAGCAGC AGCAGAGGCT 
GTGGAAAAAC AGGAAAAACC AGCCATCGCC GCAGCTGGGG AAAATCCAGT GGAGAAGCAG 
CAGAACAAGA GGAGCGACAA CGAGGAGAAT CAGAGTCAGA ATCTCAATCT GGAGGAGAAC 
GAGACGGTGC AGGAGGAGGA GAAGCTGCGC AAAGTGGTCG GCTCTGAGGC GGTGACCGCC 
GGTGAACCCG AGAAGGTGCG TCTACCCCTC CAGAAACTGG AAAGCGCCGT CAACACAGCG 
GCGTCCAAGG AGGGCGGTGC CGACGAGATA ATGCTGGCCC TGGAGTCGCA TCTGGGAGCG 
GTTAGTCGGG TCTATGGAGC CCGCAGTCTG TTCCGGCAGG ACGACATCAC CTCTGCCCTG 
AGCGCCAACT CCATTACGGG CCGCTCCGCC GGAGCCAAGT CCAAGATGCT GCTCTTCAAC 
GGCCTGTACT ACAGGGGGAG CTGGGCCACG CCCTTCTACC AACTGAGGGA TGGGAGTGAC 
GAGTTCTTCT TCATGACCAA CGAGGACGCC ATGAAGGCTC CGATGATGCA CGCGCGCGGC 
CAGTTCCTGG TGGCCGATCT GCCCCAGCTG AAGGCCCGCG TCCTGTCGCT GCCCTACGAG 
ACCTCGCGCT ACGCCCTGTG CATCCTGCTG CCCGACGAGA CGGAGGGCCT CCACGATGTC 
ATCGCCCAGA TGCAGCCCAG CGACTTCCAG CTGGGACGCG AGAAGGCGCA GCTCCGGCAG 
ATGCACGTGT CCCTGCCCAA GTTCCAGGTG GAGGAGACCT CGCGCTCCGA GTCGATGCTC 
AAGCAGATGG GCCTGCGGCG CCTCTTCTCC CGCACCGAGG CCCAGTTGGG GCTGCTCTCA 
GAGGACCCCG ACGTCCATGT GGATGAGATT GTCCAGTTCG TGAATGTCCG CGTGGACGAG 
GGCGGCAGCA GCGCCAACGC CCTCTCCGCG GCCACCCAGG CCCGCTCTCC ACAGGCTGCA 
GAGGATTCCG CCTCTGTTCT GCCCGTGCCC GAGCCGGAGC CGGAGCCGGG CGTGGAGCGG 
TTCGAGGTGA ACCGCCCCTT CGCCTACTTC ATCATGGACT GCGAGGAGCA GTTCATCCTC 
GCCTCTGGCA AGGTCTACAC GCCAGAGTTC AAGGACGATC TGCCCGGTGT TTCTGTGGAG 
ATTGAGCTGG AACAGTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA12272 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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