lox9 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	lox9
Entrez Gene ID	100037806
Full Name	lipoxygenase 9
Synonyms	GRMZM2G017616
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
NM_001112507.1	NP_001105977.1	linoleate 13S-lipoxygenase9
XM_008651174.2	XP_008649396.1	lipoxygenase 9 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the maize lipoxygenase gene family in relation to aflatoxin accumulation resistance.
Ogunola OF, Hawkins LK, Mylroie E, Kolomiets MV, Borrego E, Tang JD, Williams WP, Warburton ML
PloS one12(7)e0181265(2017)

Maize death acids, 9-lipoxygenase-derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators.
Christensen SA, Huffaker A, Kaplan F, Sims J, Ziemann S, Doehlemann G, Ji L, Schmitz RJ, Kolomiets MV, Alborn HT, Mori N, Jander G, Ni X, Sartor RC, Byers S, Abdo Z, Schmelz EA
Proceedings of the National Academy of Sciences of the United States of America112(36)11407-12(2015 Sep)

Recombinant maize 9-lipoxygenase: expression, purification, and properties.
Osipova EV, Chechetkin IR, Gogolev YV, Tarasova NB
Biochemistry. Biokhimiia75(7)861-5(2010 Jul)

The B73 maize genome: complexity, diversity, and dynamics.
Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C, Zhang J, Fulton L, Graves TA, Minx P, Reily AD, Courtney L, Kruchowski SS, Tomlinson C, Strong C, Delehaunty K, Fronick C, Courtney B, Rock SM, Belter E, Du F, Kim K, Abbott RM, Cotton M, Levy A, Marchetto P, Ochoa K, Jackson SM, Gillam B, Chen W, Yan L, Higginbotham J, Cardenas M, Waligorski J, Applebaum E, Phelps L, Falcone J, Kanchi K, Thane T, Scimone A, Thane N, Henke J, Wang T, Ruppert J, Shah N, Rotter K, Hodges J, Ingenthron E, Cordes M, Kohlberg S, Sgro J, Delgado B, Mead K, Chinwalla A, Leonard S, Crouse K, Collura K, Kudrna D, Currie J, He R, Angelova A, Rajasekar S, Mueller T, Lomeli R, Scara G, Ko A, Delaney K, Wissotski M, Lopez G, Campos D, Braidotti M, Ashley E, Golser W, Kim H, Lee S, Lin J, Dujmic Z, Kim W, Talag J, Zuccolo A, Fan C, Sebastian A, Kramer M, Spiegel L, Nascimento L, Zutavern T, Miller B, Ambroise C, Muller S, Spooner W, Narechania A, Ren L, Wei S, Kumari S, Faga B, Levy MJ, McMahan L, Van Buren P, Vaughn MW, Ying K, Yeh CT, Emrich SJ, Jia Y, Kalyanaraman A, Hsia AP, Barbazuk WB, Baucom RS, Brutnell TP, Carpita NC, Chaparro C, Chia JM, Deragon JM, Estill JC, Fu Y, Jeddeloh JA, Han Y, Lee H, Li P, Lisch DR, Liu S, Liu Z, Nagel DH, McCann MC, SanMiguel P, Myers AM, Nettleton D, Nguyen J, Penning BW, Ponnala L, Schneider KL, Schwartz DC, Sharma A, Soderlund C, Springer NM, Sun Q, Wang H, Waterman M, Westerman R, Wolfgruber TK, Yang L, Yu Y, Zhang L, Zhou S, Zhu Q, Bennetzen JL, Dawe RK, Jiang J, Jiang N, Presting GG, Wessler SR, Aluru S, Martienssen RA, Clifton SW, McCombie WR, Wing RA, Wilson RK
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)

Specificity of oxidation of linoleic acid homologs by plant lipoxygenases.
Chechetkin IR, Osipova EV, Tarasova NB, Mukhitova FK, Hamberg M, Gogolev YV, Grechkin AN
Biochemistry. Biokhimiia74(8)855-61(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lox9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lox9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OZa00291	NM_001112507.1 Latest version!	Zea mays linoleate 13S-lipoxygenase9 (lox9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OZa22862	XM_008651174.2 Latest version!	Zea mays lipoxygenase 9 (lox9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lox9 ( NM_001112507.1 ) cDNA ORF clone, Zea mays -> NP_001105977.1 Zea mays linoleate 13S-lipoxygenase9 (lox9), mRNA.

Price & Availability↑

CloneID	OZa00291
Clone ID Related Accession (Same CDS sequence)	NM_001112507.1
Accession Version	NM_001112507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526572800000
Organism	Zea mays
Product	linoleate 13S-lipoxygenase9
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ335767.1. ##Evidence-Data-START## Transcript exon combination :: DQ335767.1, BT053870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780084, SAMN00780085 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCGC CATGCAGGGA CCTCACGGGC CTTCGCCGGG GGCTTGCCGC GGCGAGGCTG 
GCGCCGGGGC GCCGGGGCGC GAACAAGCTC TGCCTCGCGC CGTTCCACCA CCGCGCGCCG 
CCGCCGCAGC AGCAGCAGGG GAGGAGGAGG CAGCGGCAGG GGCTCAAGGT GGTGGCTGCC 
ATCAGCGAGG ACCTGCCGAG GCTCGCGGCG CCCGGGACCG GGAAGGGCGC GCCGGAGGGC 
AGGCGGCCGG AGAAGGTGCT GGTGCGGGCC GCGCTCACGG TGCGCCGCAA GCACAAGGAG 
GACCTCAAGG AGGCGCTGGC CGGCCACCTC GACGCGCTAT GGGACATGGT TGGCCGGAGC 
GTCGCGCTCG AGCTCATTAG CACCAAGATC CACCCCAGGA CCAAGAAGCC GTTGCACAGC 
GGTCAGGCAT CGATCAAAGA CTGGTGCCAG AAGAGGGGCG TCAAGGGAGA GCACGTCGTG 
TACACGGCTG AGTTCATGGT GGATTCAGAC TTCGGCGAGC CTGGCGCCAT CACCGTGGCC 
AACCGGCACC ATCGGGAGTT CTTTCTGGAA AGCATCGTCG TCGAGGGTGG GCTGCCGTGT 
GGTCCGGTGC ACTTCGCCTG CAACTCCTGG GTGCAGTCCA CCAGGGAACT GCCGGGAAAG 
AGGGTGTTCT TCAGTAACAA GCCGTACTTA CCATCTGAAA CACCACCCGG GCTTAGAGAA 
CTACGGGATA AGGAATTGAA GGACCTTAGA GGAGATGGCA CAGGAGTACG GAAGCTATCT 
GACAGAATCT ATGATTATGC AACATACAAT GATTTGGGGA ATCCAGATCG GGGGAAAGAG 
TTCATCAGGC CAATCCTTGG AGGTGACAAC ATCCCTTATC CACGGCGATG CCGAACTGGT 
CGCCCACCAA CTGATACAAA CATGCTAGCT GAGAGCAGAG TAGAGAAACC TCACAGGATA 
TATGTACCTC GGGATGAGGC GTTTGAGGAG CTGAAGCAAG GTGCCTTCTC ATCAGGGAGG 
CTGCGGGCAG TACTTCATAC GCTTATCCCA TCGATGATTG CAACCATCTC AGCTGAGACC 
CACAGCTTCC AAGGCTTTCA CCACGTTGAT AATCTATACA AGGAAGGCCT CAGGCTGAAG 
TTGGGTCTCC AGGAGCACCT GTTCCAGAAA ATACCCCTTG TTCAGAAGAT TCAGGAATCA 
AGTGAGGGGA TGCTTCGCTA TGATACACCT AGAATTCTTT CCAAGGACAA GTTTGCATGG 
CTCCGTGACG ATGAGTTTGC TCGACAGACT GTGGCAGGAA TAAACCCAGT TAGCATCACC 
AGGCTCACGG TTTTCCCACC AGTAAGCAAG ATGGATCCTG CAATCTATGG CCCGCCAGAA 
TCATCGATCA CAGAAGCGCA CATCACTGGA CAGCTCAATG GACTCACGGT ACAACAGGCG 
GTGGACGAGG CAAAGCTGTT CATATTGGAC TACCATGATG TATATATGCC ATTCCTGGAC 
CGGATCAATG CAATAGAGGG GCGGAAGGCG TATGCAACCC GGACAATCTT GTTTCTTACC 
AAAGCTGGCA CATTGAAACC GATTGCTATT GAACTTAGCC TTCCTCCGAG CAAGGCAGGC 
GAGCCCCGGC CAAGCAAGGT CCTTACTCCT CCCGCTGATG CTACCTCCAA TTGGCTATGG 
ATGCTTGCCA AAGCTCATGT CAGCTCCAAC GACGCCGGTG TTCACCAGCT CGTTAATCAC 
TGGCTGAGGA CACATGCTGT AATGGAGCCG TTCATATTGG CAGCGCATCG GCGTATGAGC 
GCAATGCACC CAGTCTTCAA GCTCCTGCAC CCCCACATGC GGTACACGCT GGAGATCAAT 
GCGCTTGCAC GGCAGAGCCT GATCAGCGCG GACGGTGTCA TCGAGTCGTG CTTCACCCCT 
GGTCCCGTCT CCTTCGAGAT CAGCGCTGCA TACTACCGCG ACCACTGGCG GTTCGACCTA 
GAGGGCCTCC CTTCTGACCT CGTCCGCAGG AGAGTGGCCG TGGAGGATGC GTCGCAGCCT 
CATGGCATCA GACTCCTCAT CGAGGACTAC CCTTACGCAA ACGACGGGCT CCTGCTGTGG 
TCGGCCATTC GCAGCTGGGT GGAGTCGTAT GTGCAGCTCT ACTACCCGGA CGCCGGCACC 
GTTCAGTCCG ACGACGAGCT CCAAGGGTGG TACCACGAAA CGGTCCACGT CGGGCACGCC 
GACATCCGGC ACGCGCCCTG GTGGCCCTCG CTCTCCACGC CGGGGGACCT CGCGTCCATC 
CTGACCACGC TCGTCTGGCT CGCGTCGGCG CAGCACGCGG CGCTCAACTT CGGGCAGTAC 
CCGCTGGGCG GGTACGTCCC GAACCGCCCG CCGCTGATGC GCCGGCTGCT ACCGGACCCG 
GAGCGCGACG CCGCCGAGTA CGCGACGTTC ATGGCGGACC CGCACCGGTT CTTCCTGAAC 
GCGATGCCCG GGGTGCTGGA GGCCACCAAG TTCATGGCTG TGGTGGACAC GCTGTCGACG 
CACTCCCCCG ACGAGGAGTA CCTCGGCGAG GAGCGCGACG AGCCGTGGAC GGGCGACGCT 
GCCGCCGTGG CGGCGCACGA CATGTTCACG GCCGACGTGC GCCGCGCCGA GGAAGCCATC 
GATAGTCGAA ACGCGGACCA GCGCAGGAAG AACCGGTGCG GCGCCGGGGT GCTGCCGTAC 
GAGCTGCTGG CGCCCAGCTC GCCGCCGGGG GTCACGTGCC GCGGCGTGCC TAACAGCATC 
TCCATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001105977.1
CDS	126..2894
Translation	MAAPCRDLTGLRRGLAAARLAPGRRGANKLCLAPFHHRAPPPQQQQGRRRQRQGLKVVAAISEDLPRLAAPGTGKGAPEGRRPEKVLVRAALTVRRKHKEDLKEALAGHLDALWDMVGRSVALELISTKIHPRTKKPLHSGQASIKDWCQKRGVKGEHVVYTAEFMVDSDFGEPGAITVANRHHREFFLESIVVEGGLPCGPVHFACNSWVQSTRELPGKRVFFSNKPYLPSETPPGLRELRDKELKDLRGDGTGVRKLSDRIYDYATYNDLGNPDRGKEFIRPILGGDNIPYPRRCRTGRPPTDTNMLAESRVEKPHRIYVPRDEAFEELKQGAFSSGRLRAVLHTLIPSMIATISAETHSFQGFHHVDNLYKEGLRLKLGLQEHLFQKIPLVQKIQESSEGMLRYDTPRILSKDKFAWLRDDEFARQTVAGINPVSITRLTVFPPVSKMDPAIYGPPESSITEAHITGQLNGLTVQQAVDEAKLFILDYHDVYMPFLDRINAIEGRKAYATRTILFLTKAGTLKPIAIELSLPPSKAGEPRPSKVLTPPADATSNWLWMLAKAHVSSNDAGVHQLVNHWLRTHAVMEPFILAAHRRMSAMHPVFKLLHPHMRYTLEINALARQSLISADGVIESCFTPGPVSFEISAAYYRDHWRFDLEGLPSDLVRRRVAVEDASQPHGIRLLIEDYPYANDGLLLWSAIRSWVESYVQLYYPDAGTVQSDDELQGWYHETVHVGHADIRHAPWWPSLSTPGDLASILTTLVWLASAQHAALNFGQYPLGGYVPNRPPLMRRLLPDPERDAAEYATFMADPHRFFLNAMPGVLEATKFMAVVDTLSTHSPDEEYLGEERDEPWTGDAAAVAAHDMFTADVRRAEEAIDSRNADQRRKNRCGAGVLPYELLAPSSPPGVTCRGVPNSISI

Target ORF information:

RefSeq Version	NM_001112507.1
Organism	Zea mays
Definition	Zea mays linoleate 13S-lipoxygenase9 (lox9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112507.1

ORF Insert Sequence:

ATGGCCGCGC CATGCAGGGA CCTCACGGGC CTTCGCCGGG GGCTTGCCGC GGCGAGGCTG 
GCGCCGGGGC GCCGGGGCGC GAACAAGCTC TGCCTCGCGC CGTTCCACCA CCGCGCGCCG 
CCGCCGCAGC AGCAGCAGGG GAGGAGGAGG CAGCGGCAGG GGCTCAAGGT GGTGGCTGCC 
ATCAGCGAGG ACCTGCCGAG GCTCGCGGCG CCCGGGACCG GGAAGGGCGC GCCGGAGGGC 
AGGCGGCCGG AGAAGGTGCT GGTGCGGGCC GCGCTCACGG TGCGCCGCAA GCACAAGGAG 
GACCTCAAGG AGGCGCTGGC CGGCCACCTC GACGCGCTAT GGGACATGGT TGGCCGGAGC 
GTCGCGCTCG AGCTCATTAG CACCAAGATC CACCCCAGGA CCAAGAAGCC GTTGCACAGC 
GGTCAGGCAT CGATCAAAGA CTGGTGCCAG AAGAGGGGCG TCAAGGGAGA GCACGTCGTG 
TACACGGCTG AGTTCATGGT GGATTCAGAC TTCGGCGAGC CTGGCGCCAT CACCGTGGCC 
AACCGGCACC ATCGGGAGTT CTTTCTGGAA AGCATCGTCG TCGAGGGTGG GCTGCCGTGT 
GGTCCGGTGC ACTTCGCCTG CAACTCCTGG GTGCAGTCCA CCAGGGAACT GCCGGGAAAG 
AGGGTGTTCT TCAGTAACAA GCCGTACTTA CCATCTGAAA CACCACCCGG GCTTAGAGAA 
CTACGGGATA AGGAATTGAA GGACCTTAGA GGAGATGGCA CAGGAGTACG GAAGCTATCT 
GACAGAATCT ATGATTATGC AACATACAAT GATTTGGGGA ATCCAGATCG GGGGAAAGAG 
TTCATCAGGC CAATCCTTGG AGGTGACAAC ATCCCTTATC CACGGCGATG CCGAACTGGT 
CGCCCACCAA CTGATACAAA CATGCTAGCT GAGAGCAGAG TAGAGAAACC TCACAGGATA 
TATGTACCTC GGGATGAGGC GTTTGAGGAG CTGAAGCAAG GTGCCTTCTC ATCAGGGAGG 
CTGCGGGCAG TACTTCATAC GCTTATCCCA TCGATGATTG CAACCATCTC AGCTGAGACC 
CACAGCTTCC AAGGCTTTCA CCACGTTGAT AATCTATACA AGGAAGGCCT CAGGCTGAAG 
TTGGGTCTCC AGGAGCACCT GTTCCAGAAA ATACCCCTTG TTCAGAAGAT TCAGGAATCA 
AGTGAGGGGA TGCTTCGCTA TGATACACCT AGAATTCTTT CCAAGGACAA GTTTGCATGG 
CTCCGTGACG ATGAGTTTGC TCGACAGACT GTGGCAGGAA TAAACCCAGT TAGCATCACC 
AGGCTCACGG TTTTCCCACC AGTAAGCAAG ATGGATCCTG CAATCTATGG CCCGCCAGAA 
TCATCGATCA CAGAAGCGCA CATCACTGGA CAGCTCAATG GACTCACGGT ACAACAGGCG 
GTGGACGAGG CAAAGCTGTT CATATTGGAC TACCATGATG TATATATGCC ATTCCTGGAC 
CGGATCAATG CAATAGAGGG GCGGAAGGCG TATGCAACCC GGACAATCTT GTTTCTTACC 
AAAGCTGGCA CATTGAAACC GATTGCTATT GAACTTAGCC TTCCTCCGAG CAAGGCAGGC 
GAGCCCCGGC CAAGCAAGGT CCTTACTCCT CCCGCTGATG CTACCTCCAA TTGGCTATGG 
ATGCTTGCCA AAGCTCATGT CAGCTCCAAC GACGCCGGTG TTCACCAGCT CGTTAATCAC 
TGGCTGAGGA CACATGCTGT AATGGAGCCG TTCATATTGG CAGCGCATCG GCGTATGAGC 
GCAATGCACC CAGTCTTCAA GCTCCTGCAC CCCCACATGC GGTACACGCT GGAGATCAAT 
GCGCTTGCAC GGCAGAGCCT GATCAGCGCG GACGGTGTCA TCGAGTCGTG CTTCACCCCT 
GGTCCCGTCT CCTTCGAGAT CAGCGCTGCA TACTACCGCG ACCACTGGCG GTTCGACCTA 
GAGGGCCTCC CTTCTGACCT CGTCCGCAGG AGAGTGGCCG TGGAGGATGC GTCGCAGCCT 
CATGGCATCA GACTCCTCAT CGAGGACTAC CCTTACGCAA ACGACGGGCT CCTGCTGTGG 
TCGGCCATTC GCAGCTGGGT GGAGTCGTAT GTGCAGCTCT ACTACCCGGA CGCCGGCACC 
GTTCAGTCCG ACGACGAGCT CCAAGGGTGG TACCACGAAA CGGTCCACGT CGGGCACGCC 
GACATCCGGC ACGCGCCCTG GTGGCCCTCG CTCTCCACGC CGGGGGACCT CGCGTCCATC 
CTGACCACGC TCGTCTGGCT CGCGTCGGCG CAGCACGCGG CGCTCAACTT CGGGCAGTAC 
CCGCTGGGCG GGTACGTCCC GAACCGCCCG CCGCTGATGC GCCGGCTGCT ACCGGACCCG 
GAGCGCGACG CCGCCGAGTA CGCGACGTTC ATGGCGGACC CGCACCGGTT CTTCCTGAAC 
GCGATGCCCG GGGTGCTGGA GGCCACCAAG TTCATGGCTG TGGTGGACAC GCTGTCGACG 
CACTCCCCCG ACGAGGAGTA CCTCGGCGAG GAGCGCGACG AGCCGTGGAC GGGCGACGCT 
GCCGCCGTGG CGGCGCACGA CATGTTCACG GCCGACGTGC GCCGCGCCGA GGAAGCCATC 
GATAGTCGAA ACGCGGACCA GCGCAGGAAG AACCGGTGCG GCGCCGGGGT GCTGCCGTAC 
GAGCTGCTGG CGCCCAGCTC GCCGCCGGGG GTCACGTGCC GCGGCGTGCC TAACAGCATC 
TCCATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lox9 ( XM_008651174.2 ) cDNA ORF clone, Zea mays -> XP_008649396.1 Zea mays lipoxygenase 9 (lox9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OZa22862
Clone ID Related Accession (Same CDS sequence)	XM_008651174.2
Accession Version	XM_008651174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	lipoxygenase 9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024459.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2017 this sequence version replaced XM_008651174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGC CATGCAGGGA CCTCACGGGC CTTCGCCGGG GGCTTGCCGC GGCGAGGCTG 
GCGCCGGGGC GCCGGGGCGC GAACAAGCTC TGCCTCGCGC CGTTCCACCA CCGCGCGCCG 
CCGCCGCAGC AGCAGCAGGG GAGGAGGAGG CAGCGGCAGG GGCTCAAGGT GGTGGCTGCC 
ATCAGCGAGG ACCTGCCGAG GCTCGCGGCG CCCGGGACCG GGAAGGGCGC GCCGGAGGGC 
AGGCGGCCGG AGAAGGTGCT GGTGCGGGCC GCGCTCACGG TGCGCCGCAA GCACAAGGAG 
GACCTCAAGG AGGCGCTGGC CGGCCACCTC GACGCGCTAT GGGACATGGT TGGCCGGAGC 
GTCGCGCTCG AGCTCATTAG CACCAAGATC CACCCCAGGA CCAAGAAGCC GTTGCACAGC 
GGTCAGGCAT CGATCAAAGA CTGGTGCCAG AAGAGGGGCG TCAAGGGAGA GCACGTCGTG 
TACACGGCTG AGTTCATGGT GGATTCAGAC TTCGGCGAGC CTGGCGCCAT CACCGTGGCC 
AACCGGCACC ATCGGGAGTT CTTTCTGGAA AGCATCGTCG TCGAGGGTGG GCTGCCGTGT 
GGTCCGGTGC ACTTCGCCTG CAACTCCTGG GTGCAGTCCA CCAGGGAACT GCCGGGAAAG 
AGGGTGTTCT TCAGTAACAA GCCGTACTTA CCATCTGAAA CACCACCCGG GCTTAGAGAA 
CTACGGGATA AGGAATTGAA GGACCTTAGA GGAGATGGCA CAGGAGTACG GAAGCTATCT 
GACAGAATCT ATGATTATGC AACATACAAT GATTTGGGGA ATCCAGATCG GGGGAAAGAG 
TTCATCAGGC CAATCCTTGG AGGTGACAAC ATCCCTTATC CACGGCGATG CCGAACTGGT 
CGCCCACCAA CTGATACAAA CATGCTAGCT GAGAGCAGAG TAGAGAAACC TCACAGGATA 
TATGTACCTC GGGATGAGGC GTTTGAGGAG CTGAAGCAAG GTGCCTTCTC ATCAGGGAGG 
CTGCGGGCAG TACTTCATAC GCTTATCCCA TCGATGATTG CAACCATCTC AGCTGAGACC 
CACAGCTTCC AAGGCTTTCA CCACGTTGAT AATCTATACA AGGAAGGCCT CAGGCTGAAG 
TTGGGTCTCC AGGAGCACCT GTTCCAGAAA ATACCCCTTG TTCAGAAGAT TCAGGAATCA 
AGTGAGGGGA TGCTTCGCTA TGATACACCT AGAATTCTTT CCAAGGACAA GTTTGCATGG 
CTCCGTGACG ATGAGTTTGC TCGACAGACT GTGGCAGGAA TAAACCCAGT TAGCATCACC 
AGGCTCACGG TTTTCCCACC AGTAAGCAAG ATGGATCCTG CAATCTATGG CCCGCCAGAA 
TCATCGATCA CAGAAGCGCA CATCACTGGA CAGCTCAATG GACTCACGGT ACAACAGGCG 
GTGGACGAGG CAAAGCTGTT CATATTGGAC TACCATGATG TATATATGCC ATTCCTGGAC 
CGGATCAATG CAATAGAGGG GCGGAAGGCG TATGCAACCC GGACAATCTT GTTTCTTACC 
AAAGCTGGCA CATTGAAACC GATTGCTATT GAACTTAGCC TTCCTCCGAG CAAGGCAGGC 
GAGCCCCGGC CAAGCAAGGT CCTTACTCCT CCCGCTGATG CTACCTCCAA TTGGCTATGG 
ATGCTTGCCA AAGCTCATGT CAGCTCCAAC GACGCCGGTG TTCACCAGCT CGTTAATCAC 
TGGCTGAGGA CACATGCTGT AATGGAGCCG TTCATATTGG CAGCGCATCG GCGTATGAGC 
GCAATGCACC CAGTCTTCAA GCTCCTGCAC CCCCACATGC GGTACACGCT GGAGATCAAT 
GCGCTAGCAC GGCAGAGCCT GATCAGCGCG GACGGTGTCA TCGAGTCGTG CTTCACCCCT 
GGTCCCGTCT CCTTCGAGAT CAGCGCTGCA TACTACCGCG ACCACTGGCG GAGAGTGGCC 
GTGGAGGATG CGTCGCAGCC TCATGGCATC AGACTCCTCA TCGAGGACTA CCCTTACGCA 
AACGACGGGC TCCTGCTGTG GTCGGCCATT CGCAGCTGGG TGGAGTCGTA TGTGCAGCTC 
TACTACCCGG ACGCCGGCAC CGTTCAGTCC GACGACGAGC TCCAAGGGTG GTACCACGAA 
ACGGTCCACG TCGGGCACGC CGACATCCGG CACGCGCCCT GGTGGCCCTC GCTCTCCACG 
CCGGGGGACC TCGCGTCCAT CCTGACCACG CTCGTCTGGC TCGCGTCGGC GCAGCACGCG 
GCGCTCAACT TCGGGCAGTA CCCGCTGGGC GGGTACGTCC CGAACCGCCC GCCGCTGATG 
CGCCGGCTGC TACCGGACCC GGAGCGCGAC GCCGCCGAGT ACGCGACGTT CATGGCGGAC 
CCGCACCGGT TCTTCCTGAA CGCGATGCCC GGGGTGCTGG AGGCCACCAA GTTCATGGCT 
GTGGTGGACA CGCTGTCGAC GCACTCCCCC GACGAGGAGT ACCTCGGCGA GGAGCGCGAC 
GAGCCGTGGA CGGGCGACGC TGCCGCCGTG GCGGCGCACG ACATGTTCAC GGCCGACGTG 
CGCCGCGCCG AGGAAGCCAT CGATAGTCGA AACGCGGACC AGCGCAGGAA GAACCGGTGC 
GGCGCCGGGG TGCTGCCGTA CGAGCTGCTG GCGCCCAGCT CGCCGCCGGG GGTCACGTGC 
CGCGGCGTGC CTAACAGCAT CTCCATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008649396.1
CDS	285..3014
Translation	MAAPCRDLTGLRRGLAAARLAPGRRGANKLCLAPFHHRAPPPQQQQGRRRQRQGLKVVAAISEDLPRLAAPGTGKGAPEGRRPEKVLVRAALTVRRKHKEDLKEALAGHLDALWDMVGRSVALELISTKIHPRTKKPLHSGQASIKDWCQKRGVKGEHVVYTAEFMVDSDFGEPGAITVANRHHREFFLESIVVEGGLPCGPVHFACNSWVQSTRELPGKRVFFSNKPYLPSETPPGLRELRDKELKDLRGDGTGVRKLSDRIYDYATYNDLGNPDRGKEFIRPILGGDNIPYPRRCRTGRPPTDTNMLAESRVEKPHRIYVPRDEAFEELKQGAFSSGRLRAVLHTLIPSMIATISAETHSFQGFHHVDNLYKEGLRLKLGLQEHLFQKIPLVQKIQESSEGMLRYDTPRILSKDKFAWLRDDEFARQTVAGINPVSITRLTVFPPVSKMDPAIYGPPESSITEAHITGQLNGLTVQQAVDEAKLFILDYHDVYMPFLDRINAIEGRKAYATRTILFLTKAGTLKPIAIELSLPPSKAGEPRPSKVLTPPADATSNWLWMLAKAHVSSNDAGVHQLVNHWLRTHAVMEPFILAAHRRMSAMHPVFKLLHPHMRYTLEINALARQSLISADGVIESCFTPGPVSFEISAAYYRDHWRRVAVEDASQPHGIRLLIEDYPYANDGLLLWSAIRSWVESYVQLYYPDAGTVQSDDELQGWYHETVHVGHADIRHAPWWPSLSTPGDLASILTTLVWLASAQHAALNFGQYPLGGYVPNRPPLMRRLLPDPERDAAEYATFMADPHRFFLNAMPGVLEATKFMAVVDTLSTHSPDEEYLGEERDEPWTGDAAAVAAHDMFTADVRRAEEAIDSRNADQRRKNRCGAGVLPYELLAPSSPPGVTCRGVPNSISI

Target ORF information:

RefSeq Version	XM_008651174.2
Organism	Zea mays
Definition	Zea mays lipoxygenase 9 (lox9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008651174.2

ORF Insert Sequence:

ATGGCCGCGC CATGCAGGGA CCTCACGGGC CTTCGCCGGG GGCTTGCCGC GGCGAGGCTG 
GCGCCGGGGC GCCGGGGCGC GAACAAGCTC TGCCTCGCGC CGTTCCACCA CCGCGCGCCG 
CCGCCGCAGC AGCAGCAGGG GAGGAGGAGG CAGCGGCAGG GGCTCAAGGT GGTGGCTGCC 
ATCAGCGAGG ACCTGCCGAG GCTCGCGGCG CCCGGGACCG GGAAGGGCGC GCCGGAGGGC 
AGGCGGCCGG AGAAGGTGCT GGTGCGGGCC GCGCTCACGG TGCGCCGCAA GCACAAGGAG 
GACCTCAAGG AGGCGCTGGC CGGCCACCTC GACGCGCTAT GGGACATGGT TGGCCGGAGC 
GTCGCGCTCG AGCTCATTAG CACCAAGATC CACCCCAGGA CCAAGAAGCC GTTGCACAGC 
GGTCAGGCAT CGATCAAAGA CTGGTGCCAG AAGAGGGGCG TCAAGGGAGA GCACGTCGTG 
TACACGGCTG AGTTCATGGT GGATTCAGAC TTCGGCGAGC CTGGCGCCAT CACCGTGGCC 
AACCGGCACC ATCGGGAGTT CTTTCTGGAA AGCATCGTCG TCGAGGGTGG GCTGCCGTGT 
GGTCCGGTGC ACTTCGCCTG CAACTCCTGG GTGCAGTCCA CCAGGGAACT GCCGGGAAAG 
AGGGTGTTCT TCAGTAACAA GCCGTACTTA CCATCTGAAA CACCACCCGG GCTTAGAGAA 
CTACGGGATA AGGAATTGAA GGACCTTAGA GGAGATGGCA CAGGAGTACG GAAGCTATCT 
GACAGAATCT ATGATTATGC AACATACAAT GATTTGGGGA ATCCAGATCG GGGGAAAGAG 
TTCATCAGGC CAATCCTTGG AGGTGACAAC ATCCCTTATC CACGGCGATG CCGAACTGGT 
CGCCCACCAA CTGATACAAA CATGCTAGCT GAGAGCAGAG TAGAGAAACC TCACAGGATA 
TATGTACCTC GGGATGAGGC GTTTGAGGAG CTGAAGCAAG GTGCCTTCTC ATCAGGGAGG 
CTGCGGGCAG TACTTCATAC GCTTATCCCA TCGATGATTG CAACCATCTC AGCTGAGACC 
CACAGCTTCC AAGGCTTTCA CCACGTTGAT AATCTATACA AGGAAGGCCT CAGGCTGAAG 
TTGGGTCTCC AGGAGCACCT GTTCCAGAAA ATACCCCTTG TTCAGAAGAT TCAGGAATCA 
AGTGAGGGGA TGCTTCGCTA TGATACACCT AGAATTCTTT CCAAGGACAA GTTTGCATGG 
CTCCGTGACG ATGAGTTTGC TCGACAGACT GTGGCAGGAA TAAACCCAGT TAGCATCACC 
AGGCTCACGG TTTTCCCACC AGTAAGCAAG ATGGATCCTG CAATCTATGG CCCGCCAGAA 
TCATCGATCA CAGAAGCGCA CATCACTGGA CAGCTCAATG GACTCACGGT ACAACAGGCG 
GTGGACGAGG CAAAGCTGTT CATATTGGAC TACCATGATG TATATATGCC ATTCCTGGAC 
CGGATCAATG CAATAGAGGG GCGGAAGGCG TATGCAACCC GGACAATCTT GTTTCTTACC 
AAAGCTGGCA CATTGAAACC GATTGCTATT GAACTTAGCC TTCCTCCGAG CAAGGCAGGC 
GAGCCCCGGC CAAGCAAGGT CCTTACTCCT CCCGCTGATG CTACCTCCAA TTGGCTATGG 
ATGCTTGCCA AAGCTCATGT CAGCTCCAAC GACGCCGGTG TTCACCAGCT CGTTAATCAC 
TGGCTGAGGA CACATGCTGT AATGGAGCCG TTCATATTGG CAGCGCATCG GCGTATGAGC 
GCAATGCACC CAGTCTTCAA GCTCCTGCAC CCCCACATGC GGTACACGCT GGAGATCAAT 
GCGCTAGCAC GGCAGAGCCT GATCAGCGCG GACGGTGTCA TCGAGTCGTG CTTCACCCCT 
GGTCCCGTCT CCTTCGAGAT CAGCGCTGCA TACTACCGCG ACCACTGGCG GAGAGTGGCC 
GTGGAGGATG CGTCGCAGCC TCATGGCATC AGACTCCTCA TCGAGGACTA CCCTTACGCA 
AACGACGGGC TCCTGCTGTG GTCGGCCATT CGCAGCTGGG TGGAGTCGTA TGTGCAGCTC 
TACTACCCGG ACGCCGGCAC CGTTCAGTCC GACGACGAGC TCCAAGGGTG GTACCACGAA 
ACGGTCCACG TCGGGCACGC CGACATCCGG CACGCGCCCT GGTGGCCCTC GCTCTCCACG 
CCGGGGGACC TCGCGTCCAT CCTGACCACG CTCGTCTGGC TCGCGTCGGC GCAGCACGCG 
GCGCTCAACT TCGGGCAGTA CCCGCTGGGC GGGTACGTCC CGAACCGCCC GCCGCTGATG 
CGCCGGCTGC TACCGGACCC GGAGCGCGAC GCCGCCGAGT ACGCGACGTT CATGGCGGAC 
CCGCACCGGT TCTTCCTGAA CGCGATGCCC GGGGTGCTGG AGGCCACCAA GTTCATGGCT 
GTGGTGGACA CGCTGTCGAC GCACTCCCCC GACGAGGAGT ACCTCGGCGA GGAGCGCGAC 
GAGCCGTGGA CGGGCGACGC TGCCGCCGTG GCGGCGCACG ACATGTTCAC GGCCGACGTG 
CGCCGCGCCG AGGAAGCCAT CGATAGTCGA AACGCGGACC AGCGCAGGAA GAACCGGTGC 
GGCGCCGGGG TGCTGCCGTA CGAGCTGCTG GCGCCCAGCT CGCCGCCGGG GGTCACGTGC 
CGCGGCGTGC CTAACAGCAT CTCCATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of the maize lipoxygenase gene family in relation to aflatoxin accumulation resistance.
PloS one12(7)e0181265(2017)
Ogunola OF,Hawkins LK,Mylroie E,Kolomiets MV,Borrego E,Tang JD,Williams WP,Warburton ML

Maize death acids, 9-lipoxygenase-derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators.
Proceedings of the National Academy of Sciences of the United States of America112(36)11407-12(2015 Sep)
Christensen SA,Huffaker A,Kaplan F,Sims J,Ziemann S,Doehlemann G,Ji L,Schmitz RJ,Kolomiets MV,Alborn HT,Mori N,Jander G,Ni X,Sartor RC,Byers S,Abdo Z,Schmelz EA

Recombinant maize 9-lipoxygenase: expression, purification, and properties.
Biochemistry. Biokhimiia75(7)861-5(2010 Jul)
Osipova EV,Chechetkin IR,Gogolev YV,Tarasova NB

The B73 maize genome: complexity, diversity, and dynamics.
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)
Schnable PS,Ware D,Fulton RS,Stein JC,Wei F,Pasternak S,Liang C,Zhang J,Fulton L,Graves TA,Minx P,Reily AD,Courtney L,Kruchowski SS,Tomlinson C,Strong C,Delehaunty K,Fronick C,Courtney B,Rock SM,Belter E,Du F,Kim K,Abbott RM,Cotton M,Levy A,Marchetto P,Ochoa K,Jackson SM,Gillam B,Chen W,Yan L,Higginbotham J,Cardenas M,Waligorski J,Applebaum E,Phelps L,Falcone J,Kanchi K,Thane T,Scimone A,Thane N,Henke J,Wang T,Ruppert J,Shah N,Rotter K,Hodges J,Ingenthron E,Cordes M,Kohlberg S,Sgro J,Delgado B,Mead K,Chinwalla A,Leonard S,Crouse K,Collura K,Kudrna D,Currie J,He R,Angelova A,Rajasekar S,Mueller T,Lomeli R,Scara G,Ko A,Delaney K,Wissotski M,Lopez G,Campos D,Braidotti M,Ashley E,Golser W,Kim H,Lee S,Lin J,Dujmic Z,Kim W,Talag J,Zuccolo A,Fan C,Sebastian A,Kramer M,Spiegel L,Nascimento L,Zutavern T,Miller B,Ambroise C,Muller S,Spooner W,Narechania A,Ren L,Wei S,Kumari S,Faga B,Levy MJ,McMahan L,Van Buren P,Vaughn MW,Ying K,Yeh CT,Emrich SJ,Jia Y,Kalyanaraman A,Hsia AP,Barbazuk WB,Baucom RS,Brutnell TP,Carpita NC,Chaparro C,Chia JM,Deragon JM,Estill JC,Fu Y,Jeddeloh JA,Han Y,Lee H,Li P,Lisch DR,Liu S,Liu Z,Nagel DH,McCann MC,SanMiguel P,Myers AM,Nettleton D,Nguyen J,Penning BW,Ponnala L,Schneider KL,Schwartz DC,Sharma A,Soderlund C,Springer NM,Sun Q,Wang H,Waterman M,Westerman R,Wolfgruber TK,Yang L,Yu Y,Zhang L,Zhou S,Zhu Q,Bennetzen JL,Dawe RK,Jiang J,Jiang N,Presting GG,Wessler SR,Aluru S,Martienssen RA,Clifton SW,McCombie WR,Wing RA,Wilson RK

Specificity of oxidation of linoleic acid homologs by plant lipoxygenases.
Biochemistry. Biokhimiia74(8)855-61(2009 Aug)
Chechetkin IR,Osipova EV,Tarasova NB,Mukhitova FK,Hamberg M,Gogolev YV,Grechkin AN

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lox9 cDNA ORF clone, Zea mays

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Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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