LOC8064184 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8064184
Entrez Gene ID	8064184
Full Name	probable calcium-transporting ATPase 6, plasma membrane-type
Gene Type	protein-coding
Organism	Sorghum bicolor(sorghum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002439961.1	XP_002440006.1	hypothetical protein
XM_002439961.2	XP_002440006.1	probable calcium-transporting ATPase 6, plasma membrane-type

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Sorghum bicolor genome and the diversification of grasses.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KF, Messing J, Rokhsar DS
Nature457(7229)551-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC8064184 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8064184 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc05108	XM_002439961.2 Latest version!	Sorghum bicolor probable calcium-transporting ATPase 6, plasma membrane-type (LOC8064184), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OSc05108	XM_002439961.1	Sorghum bicolor hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8064184 ( XM_002439961.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002440006.1 Sorghum bicolor hypothetical protein, mRNA.

Price & Availability↑

CloneID	OSc05108
Clone ID Related Accession (Same CDS sequence)	XM_002439961.1 , XM_002439961.2
Accession Version	XM_002439961.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1247328000000
Organism	Sorghum bicolor(Sorghum)
Product	hypothetical protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012878). COMPLETENESS: incomplete on both ends.

ATGGAGTCGA GCTCGAGCAG GAGCTGGAGC ATCGACAGCT ACCTCAACGA GCACTTCGAC 
ATCCCCGCCA AGAACCCGCC CAGCGAGGCC CGCCTCCGGT GGCGCCGCGC CGTCGGCCTC 
GTCGTCCGCA ACCGTCGCCG CCGCTTCCGC ATGTTCTCCG GCCTCCACGC CCTCGACGAC 
GCCCAGCGCC GCAAGATCCT GGGAAAAGTT CAGGTTGTAA TTAATGTGCA CAAGGCAGCG 
CTGCATTTTA TTGACGGTAT AAGACGATAC CACCTATCTC CTGAGCTTAT CGAAGCAGGA 
TTTTGTATCA GTCCAGATGA ACTGGCAGCA ATTACTGGAA TTCGTGAAGA TTATACAATC 
TTCAAGACTC ATGGTGGAAT TAGTGGTATA TCTAGGAAAA TCAAAGCATC ATTGGAAGAT 
GGCATCAAGG AAACTGAAAT AGCAACCAGG CAGAAACTGT ATGGCAGTAA TAAGCATGCT 
GAGAAGCCTC CTAGAAGCTT CTGGATGTTT GTCTGGGATG CATTGCATGA CCTGACGTTG 
ATTATTCTAA TTGTTTGTGC TGTTGTTTCT CTAGTAGTTG GCCTTGCCAC TGAGGGGTGG 
CCGAAGGGCA TCTATGACGG CCTTGGGATA ATAACCAGCA TTTTATTGGT GGTGCTGGTT 
ACAGCATCCA GTGATTACAA ACAGTCACGG AAATTCATGG AGCTGGACTG TGAGAAAAAG 
AAGATATATG CCCTTGTCAC TAGAGATAGG AAAACCAAAA GGGTACTGAT TCATGATTTG 
GTTGTGGGTG ACATCTTGCA TCTTTCAATA GGTGATGTGG TTCCTGCAGA TGGCCTGTTC 
ATCTCTGGCT ACTGCTTAGT CATAGATGAA TCTAGTTTAT CAGGTGAAAG TGAACCAGTT 
CATGTTTTTG AAGAAAAGCC TTTCATTCAT GCTGGAAGTA AAGTGGTAGA TGGGACTGCC 
AAGATGCTTG TAACTGCTGT TGGTATGCGT ACTGAATGGG GCAAAATCAT GGATACTCTG 
AATGATGATG GCGTGGATGA AACCCCTTTA CAAGTTAAGC TTAATGGTGT GGCAACAATC 
ATTGGACAGA TCGGGTTGGT GTTTGCTATC CTCACATTTT TGGTACTTCT AGTGAGGTTC 
CTTGTTGACA AGGGGAAGGA CGTTGGGTTG TTAAATTGGT CAGCAAATGA TGCACTGACA 
ATAGTGAACT ACTTTGCTAT TGCAGTGACA ATCATTGTTG TCGCAGTCCC TGAGGGTCTA 
CCACTGGCTG TCACTCTTAG CCTTGCGTTT GCCATGCAAA AGTTGATGAA TGATAAAGCA 
CTAGTGAGGC ATCTCGCTGC ATGTGAAACC ATGGGTTCAG CTAGTTGTAT TTGCACTGAT 
AAGACTGGAA CTTTGACGAC CAACCACATG ATCGTTGACA AGGTTTGGAT CGGTGATGTA 
TCCAAGTCTG TCAATGGTGA TACAAATATG AATGAGCTGA AGGCTGCTAC TGCCGAAAGT 
GCTGTGGACA TACTTATTCA AGGAATATTT GTGAACACTG GTTCTGAGAT CGTGAAGGGA 
GACGATGGCA AAAAAACTAT CTTAGGCACA CCAACTGAAG CAGCATTATT GGAGTTTGGC 
TTGATCTTGC AAGGAGACCT GTATGGTGAG TACAATAAGT TGGCAAGAGT CAAAGTAGAG 
CCTTTTAACT CTGTTAAGAA AAAGATGTCT GTGCTAGTAC AGTTACCTAA TGGAGGCCTC 
CGGTCCTTCT GTAAAGGTGC ATCGGAACTT ATTTTAGGGC AGTGTGATAC TTTCCTCAAT 
AGTGAAGGAA ATTTAGCACC ACTGTCAGAA ATGCAAAAGC AAAATGTCTT AAATATAATA 
AACTCATTTG CTTCTGAGGC ATTAAGAACG CTTTGTATTG CATTTAAGGA TCTGAGTGAA 
ATTCCTGATG ACCAGACAAT ACCAGAGGAT GGTTACACAC TAATAGCACT TTTTGGCATA 
AAGGATCCAG TCCGTCCCGG TGTAAGGGAT GCAGTAATGA CCTGCATGGC TGCTGGAATC 
AAAGTAAAAA TGGTGACTGG AGACAACATC AATACTGCTA AAGCCATTGC CAAGGAATGT 
GGAATATTGA CCGAGGATGG AATAGCAATC GAAGGAAGAG AGCTTCACGA TAAGAGCGCA 
GATGAATTGA AGGAGATCCT GCCTAAAATT CAGGTAATGG CCCGGTCCTT GCCGATGGAC 
AAATATAAAT TGGTGACAAG CCTAAAAAGT ATGTATCAAG AGGTCGTGGC AGTTACTGGT 
GATGGAACAA ACGATGCTCC AGCATTGCGT GAGTCAGACA TAGGTCTGGC CATGGGCATT 
GCCGGCACTG AGGTTGCGAA AGAAAATGCC GATGTTATAA TAATGGATGA TAATTTCAGT 
ACCATTGTAA ATGTTGCTAG ATGGGGTCGT GCGGTTTACT TGAACATTCA GAAGTTTGTG 
CAGTTCCAGC TTACAGTTAA TATAGTGGCT CTGATAGTGA ATTTCATCTC AGCGTGTATC 
ATAGGTACTG CACCACTTAC TGCCGTTCAG TTGCTATGGG TGAACATGAT CATGGATACA 
TTAGGAGCCT TGGCCTTAGC GACAGAGCCA CCTAATGATG AAATGATGAA GAGACCACCT 
GTGAGACGGG GGCATGGTTT CATCACTCAG GTCATGTGGA GAAATATTCT TGGGCAGGCT 
TTATACCAGC TCCTTGTACT TGGTACTCTC ATGTTTGTTG GTAAGAGGAT CCTTAATATC 
GAAGGCCCAA ATGCTGATAT AACGATCAAT ACCCTCATAT TCAACTCTTT TGTGTTTTGC 
CAGGTTTTCA ACGAGATAAA TAGCAGGGAA ATGGAAAAGA TCAACGTGTT CAGAGGGATA 
CTGAAAAATT GGATCTTTAT CAGTATATTG ACAGCAACCG TCGTATTCCA AGTAATTATA 
GTGGAGTTTC TTGGAACCTT TGCAAACACC ATACCGCTGA GTTGGAAGCT GTGGTTGCTC 
AGTATTATTC TTGGCTCGGT CAGTATGGTC ATCTCTGTTA TCGTCAAGTG CATTCCCGTC 
GAATCTAGAA AAACAAATAT TAAGCCCCAT GGCTATGAGT TGATTCCTGA AGCCCCAGAA 
ACAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002440006.1
CDS	1..3129
Translation	MESSSSRSWSIDSYLNEHFDIPAKNPPSEARLRWRRAVGLVVRNRRRRFRMFSGLHALDDAQRRKILGKVQVVINVHKAALHFIDGIRRYHLSPELIEAGFCISPDELAAITGIREDYTIFKTHGGISGISRKIKASLEDGIKETEIATRQKLYGSNKHAEKPPRSFWMFVWDALHDLTLIILIVCAVVSLVVGLATEGWPKGIYDGLGIITSILLVVLVTASSDYKQSRKFMELDCEKKKIYALVTRDRKTKRVLIHDLVVGDILHLSIGDVVPADGLFISGYCLVIDESSLSGESEPVHVFEEKPFIHAGSKVVDGTAKMLVTAVGMRTEWGKIMDTLNDDGVDETPLQVKLNGVATIIGQIGLVFAILTFLVLLVRFLVDKGKDVGLLNWSANDALTIVNYFAIAVTIIVVAVPEGLPLAVTLSLAFAMQKLMNDKALVRHLAACETMGSASCICTDKTGTLTTNHMIVDKVWIGDVSKSVNGDTNMNELKAATAESAVDILIQGIFVNTGSEIVKGDDGKKTILGTPTEAALLEFGLILQGDLYGEYNKLARVKVEPFNSVKKKMSVLVQLPNGGLRSFCKGASELILGQCDTFLNSEGNLAPLSEMQKQNVLNIINSFASEALRTLCIAFKDLSEIPDDQTIPEDGYTLIALFGIKDPVRPGVRDAVMTCMAAGIKVKMVTGDNINTAKAIAKECGILTEDGIAIEGRELHDKSADELKEILPKIQVMARSLPMDKYKLVTSLKSMYQEVVAVTGDGTNDAPALRESDIGLAMGIAGTEVAKENADVIIMDDNFSTIVNVARWGRAVYLNIQKFVQFQLTVNIVALIVNFISACIIGTAPLTAVQLLWVNMIMDTLGALALATEPPNDEMMKRPPVRRGHGFITQVMWRNILGQALYQLLVLGTLMFVGKRILNIEGPNADITINTLIFNSFVFCQVFNEINSREMEKINVFRGILKNWIFISILTATVVFQVIIVEFLGTFANTIPLSWKLWLLSIILGSVSMVISVIVKCIPVESRKTNIKPHGYELIPEAPETV

Target ORF information:

RefSeq Version	XM_002439961.1
Organism	Sorghum bicolor(Sorghum)
Definition	Sorghum bicolor hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002439961.1

ORF Insert Sequence:

ATGGAGTCGA GCTCGAGCAG GAGCTGGAGC ATCGACAGCT ACCTCAACGA GCACTTCGAC 
ATCCCCGCCA AGAACCCGCC CAGCGAGGCC CGCCTCCGGT GGCGCCGCGC CGTCGGCCTC 
GTCGTCCGCA ACCGTCGCCG CCGCTTCCGC ATGTTCTCCG GCCTCCACGC CCTCGACGAC 
GCCCAGCGCC GCAAGATCCT GGGAAAAGTT CAGGTTGTAA TTAATGTGCA CAAGGCAGCG 
CTGCATTTTA TTGACGGTAT AAGACGATAC CACCTATCTC CTGAGCTTAT CGAAGCAGGA 
TTTTGTATCA GTCCAGATGA ACTGGCAGCA ATTACTGGAA TTCGTGAAGA TTATACAATC 
TTCAAGACTC ATGGTGGAAT TAGTGGTATA TCTAGGAAAA TCAAAGCATC ATTGGAAGAT 
GGCATCAAGG AAACTGAAAT AGCAACCAGG CAGAAACTGT ATGGCAGTAA TAAGCATGCT 
GAGAAGCCTC CTAGAAGCTT CTGGATGTTT GTCTGGGATG CATTGCATGA CCTGACGTTG 
ATTATTCTAA TTGTTTGTGC TGTTGTTTCT CTAGTAGTTG GCCTTGCCAC TGAGGGGTGG 
CCGAAGGGCA TCTATGACGG CCTTGGGATA ATAACCAGCA TTTTATTGGT GGTGCTGGTT 
ACAGCATCCA GTGATTACAA ACAGTCACGG AAATTCATGG AGCTGGACTG TGAGAAAAAG 
AAGATATATG CCCTTGTCAC TAGAGATAGG AAAACCAAAA GGGTACTGAT TCATGATTTG 
GTTGTGGGTG ACATCTTGCA TCTTTCAATA GGTGATGTGG TTCCTGCAGA TGGCCTGTTC 
ATCTCTGGCT ACTGCTTAGT CATAGATGAA TCTAGTTTAT CAGGTGAAAG TGAACCAGTT 
CATGTTTTTG AAGAAAAGCC TTTCATTCAT GCTGGAAGTA AAGTGGTAGA TGGGACTGCC 
AAGATGCTTG TAACTGCTGT TGGTATGCGT ACTGAATGGG GCAAAATCAT GGATACTCTG 
AATGATGATG GCGTGGATGA AACCCCTTTA CAAGTTAAGC TTAATGGTGT GGCAACAATC 
ATTGGACAGA TCGGGTTGGT GTTTGCTATC CTCACATTTT TGGTACTTCT AGTGAGGTTC 
CTTGTTGACA AGGGGAAGGA CGTTGGGTTG TTAAATTGGT CAGCAAATGA TGCACTGACA 
ATAGTGAACT ACTTTGCTAT TGCAGTGACA ATCATTGTTG TCGCAGTCCC TGAGGGTCTA 
CCACTGGCTG TCACTCTTAG CCTTGCGTTT GCCATGCAAA AGTTGATGAA TGATAAAGCA 
CTAGTGAGGC ATCTCGCTGC ATGTGAAACC ATGGGTTCAG CTAGTTGTAT TTGCACTGAT 
AAGACTGGAA CTTTGACGAC CAACCACATG ATCGTTGACA AGGTTTGGAT CGGTGATGTA 
TCCAAGTCTG TCAATGGTGA TACAAATATG AATGAGCTGA AGGCTGCTAC TGCCGAAAGT 
GCTGTGGACA TACTTATTCA AGGAATATTT GTGAACACTG GTTCTGAGAT CGTGAAGGGA 
GACGATGGCA AAAAAACTAT CTTAGGCACA CCAACTGAAG CAGCATTATT GGAGTTTGGC 
TTGATCTTGC AAGGAGACCT GTATGGTGAG TACAATAAGT TGGCAAGAGT CAAAGTAGAG 
CCTTTTAACT CTGTTAAGAA AAAGATGTCT GTGCTAGTAC AGTTACCTAA TGGAGGCCTC 
CGGTCCTTCT GTAAAGGTGC ATCGGAACTT ATTTTAGGGC AGTGTGATAC TTTCCTCAAT 
AGTGAAGGAA ATTTAGCACC ACTGTCAGAA ATGCAAAAGC AAAATGTCTT AAATATAATA 
AACTCATTTG CTTCTGAGGC ATTAAGAACG CTTTGTATTG CATTTAAGGA TCTGAGTGAA 
ATTCCTGATG ACCAGACAAT ACCAGAGGAT GGTTACACAC TAATAGCACT TTTTGGCATA 
AAGGATCCAG TCCGTCCCGG TGTAAGGGAT GCAGTAATGA CCTGCATGGC TGCTGGAATC 
AAAGTAAAAA TGGTGACTGG AGACAACATC AATACTGCTA AAGCCATTGC CAAGGAATGT 
GGAATATTGA CCGAGGATGG AATAGCAATC GAAGGAAGAG AGCTTCACGA TAAGAGCGCA 
GATGAATTGA AGGAGATCCT GCCTAAAATT CAGGTAATGG CCCGGTCCTT GCCGATGGAC 
AAATATAAAT TGGTGACAAG CCTAAAAAGT ATGTATCAAG AGGTCGTGGC AGTTACTGGT 
GATGGAACAA ACGATGCTCC AGCATTGCGT GAGTCAGACA TAGGTCTGGC CATGGGCATT 
GCCGGCACTG AGGTTGCGAA AGAAAATGCC GATGTTATAA TAATGGATGA TAATTTCAGT 
ACCATTGTAA ATGTTGCTAG ATGGGGTCGT GCGGTTTACT TGAACATTCA GAAGTTTGTG 
CAGTTCCAGC TTACAGTTAA TATAGTGGCT CTGATAGTGA ATTTCATCTC AGCGTGTATC 
ATAGGTACTG CACCACTTAC TGCCGTTCAG TTGCTATGGG TGAACATGAT CATGGATACA 
TTAGGAGCCT TGGCCTTAGC GACAGAGCCA CCTAATGATG AAATGATGAA GAGACCACCT 
GTGAGACGGG GGCATGGTTT CATCACTCAG GTCATGTGGA GAAATATTCT TGGGCAGGCT 
TTATACCAGC TCCTTGTACT TGGTACTCTC ATGTTTGTTG GTAAGAGGAT CCTTAATATC 
GAAGGCCCAA ATGCTGATAT AACGATCAAT ACCCTCATAT TCAACTCTTT TGTGTTTTGC 
CAGGTTTTCA ACGAGATAAA TAGCAGGGAA ATGGAAAAGA TCAACGTGTT CAGAGGGATA 
CTGAAAAATT GGATCTTTAT CAGTATATTG ACAGCAACCG TCGTATTCCA AGTAATTATA 
GTGGAGTTTC TTGGAACCTT TGCAAACACC ATACCGCTGA GTTGGAAGCT GTGGTTGCTC 
AGTATTATTC TTGGCTCGGT CAGTATGGTC ATCTCTGTTA TCGTCAAGTG CATTCCCGTC 
GAATCTAGAA AAACAAATAT TAAGCCCCAT GGCTATGAGT TGATTCCTGA AGCCCCAGAA 
ACAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8064184 ( XM_002439961.2 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002440006.1 Sorghum bicolor probable calcium-transporting ATPase 6, plasma membrane-type (LOC8064184), mRNA.

Price & Availability↑

CloneID	OSc05108
Clone ID Related Accession (Same CDS sequence)	XM_002439961.1 , XM_002439961.2
Accession Version	XM_002439961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	probable calcium-transporting ATPase 6, plasma membrane-type
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012878.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 13, 2017 this sequence version replaced XM_002439961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGA GCTCGAGCAG GAGCTGGAGC ATCGACAGCT ACCTCAACGA GCACTTCGAC 
ATCCCCGCCA AGAACCCGCC CAGCGAGGCC CGCCTCCGGT GGCGCCGCGC CGTCGGCCTC 
GTCGTCCGCA ACCGTCGCCG CCGCTTCCGC ATGTTCTCCG GCCTCCACGC CCTCGACGAC 
GCCCAGCGCC GCAAGATCCT GGGAAAAGTT CAGGTTGTAA TTAATGTGCA CAAGGCAGCG 
CTGCATTTTA TTGACGGTAT AAGACGATAC CACCTATCTC CTGAGCTTAT CGAAGCAGGA 
TTTTGTATCA GTCCAGATGA ACTGGCAGCA ATTACTGGAA TTCGTGAAGA TTATACAATC 
TTCAAGACTC ATGGTGGAAT TAGTGGTATA TCTAGGAAAA TCAAAGCATC ATTGGAAGAT 
GGCATCAAGG AAACTGAAAT AGCAACCAGG CAGAAACTGT ATGGCAGTAA TAAGCATGCT 
GAGAAGCCTC CTAGAAGCTT CTGGATGTTT GTCTGGGATG CATTGCATGA CCTGACGTTG 
ATTATTCTAA TTGTTTGTGC TGTTGTTTCT CTAGTAGTTG GCCTTGCCAC TGAGGGGTGG 
CCGAAGGGCA TCTATGACGG CCTTGGGATA ATAACCAGCA TTTTATTGGT GGTGCTGGTT 
ACAGCATCCA GTGATTACAA ACAGTCACGG AAATTCATGG AGCTGGACTG TGAGAAAAAG 
AAGATATATG CCCTTGTCAC TAGAGATAGG AAAACCAAAA GGGTACTGAT TCATGATTTG 
GTTGTGGGTG ACATCTTGCA TCTTTCAATA GGTGATGTGG TTCCTGCAGA TGGCCTGTTC 
ATCTCTGGCT ACTGCTTAGT CATAGATGAA TCTAGTTTAT CAGGTGAAAG TGAACCAGTT 
CATGTTTTTG AAGAAAAGCC TTTCATTCAT GCTGGAAGTA AAGTGGTAGA TGGGACTGCC 
AAGATGCTTG TAACTGCTGT TGGTATGCGT ACTGAATGGG GCAAAATCAT GGATACTCTG 
AATGATGATG GCGTGGATGA AACCCCTTTA CAAGTTAAGC TTAATGGTGT GGCAACAATC 
ATTGGACAGA TCGGGTTGGT GTTTGCTATC CTCACATTTT TGGTACTTCT AGTGAGGTTC 
CTTGTTGACA AGGGGAAGGA CGTTGGGTTG TTAAATTGGT CAGCAAATGA TGCACTGACA 
ATAGTGAACT ACTTTGCTAT TGCAGTGACA ATCATTGTTG TCGCAGTCCC TGAGGGTCTA 
CCACTGGCTG TCACTCTTAG CCTTGCGTTT GCCATGCAAA AGTTGATGAA TGATAAAGCA 
CTAGTGAGGC ATCTCGCTGC ATGTGAAACC ATGGGTTCAG CTAGTTGTAT TTGCACTGAT 
AAGACTGGAA CTTTGACGAC CAACCACATG ATCGTTGACA AGGTTTGGAT CGGTGATGTA 
TCCAAGTCTG TCAATGGTGA TACAAATATG AATGAGCTGA AGGCTGCTAC TGCCGAAAGT 
GCTGTGGACA TACTTATTCA AGGAATATTT GTGAACACTG GTTCTGAGAT CGTGAAGGGA 
GACGATGGCA AAAAAACTAT CTTAGGCACA CCAACTGAAG CAGCATTATT GGAGTTTGGC 
TTGATCTTGC AAGGAGACCT GTATGGTGAG TACAATAAGT TGGCAAGAGT CAAAGTAGAG 
CCTTTTAACT CTGTTAAGAA AAAGATGTCT GTGCTAGTAC AGTTACCTAA TGGAGGCCTC 
CGGTCCTTCT GTAAAGGTGC ATCGGAACTT ATTTTAGGGC AGTGTGATAC TTTCCTCAAT 
AGTGAAGGAA ATTTAGCACC ACTGTCAGAA ATGCAAAAGC AAAATGTCTT AAATATAATA 
AACTCATTTG CTTCTGAGGC ATTAAGAACG CTTTGTATTG CATTTAAGGA TCTGAGTGAA 
ATTCCTGATG ACCAGACAAT ACCAGAGGAT GGTTACACAC TAATAGCACT TTTTGGCATA 
AAGGATCCAG TCCGTCCCGG TGTAAGGGAT GCAGTAATGA CCTGCATGGC TGCTGGAATC 
AAAGTAAAAA TGGTGACTGG AGACAACATC AATACTGCTA AAGCCATTGC CAAGGAATGT 
GGAATATTGA CCGAGGATGG AATAGCAATC GAAGGAAGAG AGCTTCACGA TAAGAGCGCA 
GATGAATTGA AGGAGATCCT GCCTAAAATT CAGGTAATGG CCCGGTCCTT GCCGATGGAC 
AAATATAAAT TGGTGACAAG CCTAAAAAGT ATGTATCAAG AGGTCGTGGC AGTTACTGGT 
GATGGAACAA ACGATGCTCC AGCATTGCGT GAGTCAGACA TAGGTCTGGC CATGGGCATT 
GCCGGCACTG AGGTTGCGAA AGAAAATGCC GATGTTATAA TAATGGATGA TAATTTCAGT 
ACCATTGTAA ATGTTGCTAG ATGGGGTCGT GCGGTTTACT TGAACATTCA GAAGTTTGTG 
CAGTTCCAGC TTACAGTTAA TATAGTGGCT CTGATAGTGA ATTTCATCTC AGCGTGTATC 
ATAGGTACTG CACCACTTAC TGCCGTTCAG TTGCTATGGG TGAACATGAT CATGGATACA 
TTAGGAGCCT TGGCCTTAGC GACAGAGCCA CCTAATGATG AAATGATGAA GAGACCACCT 
GTGAGACGGG GGCATGGTTT CATCACTCAG GTCATGTGGA GAAATATTCT TGGGCAGGCT 
TTATACCAGC TCCTTGTACT TGGTACTCTC ATGTTTGTTG GTAAGAGGAT CCTTAATATC 
GAAGGCCCAA ATGCTGATAT AACGATCAAT ACCCTCATAT TCAACTCTTT TGTGTTTTGC 
CAGGTTTTCA ACGAGATAAA TAGCAGGGAA ATGGAAAAGA TCAACGTGTT CAGAGGGATA 
CTGAAAAATT GGATCTTTAT CAGTATATTG ACAGCAACCG TCGTATTCCA AGTAATTATA 
GTGGAGTTTC TTGGAACCTT TGCAAACACC ATACCGCTGA GTTGGAAGCT GTGGTTGCTC 
AGTATTATTC TTGGCTCGGT CAGTATGGTC ATCTCTGTTA TCGTCAAGTG CATTCCCGTC 
GAATCTAGAA AAACAAATAT TAAGCCCCAT GGCTATGAGT TGATTCCTGA AGCCCCAGAA 
ACAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002440006.1
CDS	188..3316
Translation	MESSSSRSWSIDSYLNEHFDIPAKNPPSEARLRWRRAVGLVVRNRRRRFRMFSGLHALDDAQRRKILGKVQVVINVHKAALHFIDGIRRYHLSPELIEAGFCISPDELAAITGIREDYTIFKTHGGISGISRKIKASLEDGIKETEIATRQKLYGSNKHAEKPPRSFWMFVWDALHDLTLIILIVCAVVSLVVGLATEGWPKGIYDGLGIITSILLVVLVTASSDYKQSRKFMELDCEKKKIYALVTRDRKTKRVLIHDLVVGDILHLSIGDVVPADGLFISGYCLVIDESSLSGESEPVHVFEEKPFIHAGSKVVDGTAKMLVTAVGMRTEWGKIMDTLNDDGVDETPLQVKLNGVATIIGQIGLVFAILTFLVLLVRFLVDKGKDVGLLNWSANDALTIVNYFAIAVTIIVVAVPEGLPLAVTLSLAFAMQKLMNDKALVRHLAACETMGSASCICTDKTGTLTTNHMIVDKVWIGDVSKSVNGDTNMNELKAATAESAVDILIQGIFVNTGSEIVKGDDGKKTILGTPTEAALLEFGLILQGDLYGEYNKLARVKVEPFNSVKKKMSVLVQLPNGGLRSFCKGASELILGQCDTFLNSEGNLAPLSEMQKQNVLNIINSFASEALRTLCIAFKDLSEIPDDQTIPEDGYTLIALFGIKDPVRPGVRDAVMTCMAAGIKVKMVTGDNINTAKAIAKECGILTEDGIAIEGRELHDKSADELKEILPKIQVMARSLPMDKYKLVTSLKSMYQEVVAVTGDGTNDAPALRESDIGLAMGIAGTEVAKENADVIIMDDNFSTIVNVARWGRAVYLNIQKFVQFQLTVNIVALIVNFISACIIGTAPLTAVQLLWVNMIMDTLGALALATEPPNDEMMKRPPVRRGHGFITQVMWRNILGQALYQLLVLGTLMFVGKRILNIEGPNADITINTLIFNSFVFCQVFNEINSREMEKINVFRGILKNWIFISILTATVVFQVIIVEFLGTFANTIPLSWKLWLLSIILGSVSMVISVIVKCIPVESRKTNIKPHGYELIPEAPETV

Target ORF information:

RefSeq Version	XM_002439961.2
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor probable calcium-transporting ATPase 6, plasma membrane-type (LOC8064184), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002439961.2

ORF Insert Sequence:

ATGGAGTCGA GCTCGAGCAG GAGCTGGAGC ATCGACAGCT ACCTCAACGA GCACTTCGAC 
ATCCCCGCCA AGAACCCGCC CAGCGAGGCC CGCCTCCGGT GGCGCCGCGC CGTCGGCCTC 
GTCGTCCGCA ACCGTCGCCG CCGCTTCCGC ATGTTCTCCG GCCTCCACGC CCTCGACGAC 
GCCCAGCGCC GCAAGATCCT GGGAAAAGTT CAGGTTGTAA TTAATGTGCA CAAGGCAGCG 
CTGCATTTTA TTGACGGTAT AAGACGATAC CACCTATCTC CTGAGCTTAT CGAAGCAGGA 
TTTTGTATCA GTCCAGATGA ACTGGCAGCA ATTACTGGAA TTCGTGAAGA TTATACAATC 
TTCAAGACTC ATGGTGGAAT TAGTGGTATA TCTAGGAAAA TCAAAGCATC ATTGGAAGAT 
GGCATCAAGG AAACTGAAAT AGCAACCAGG CAGAAACTGT ATGGCAGTAA TAAGCATGCT 
GAGAAGCCTC CTAGAAGCTT CTGGATGTTT GTCTGGGATG CATTGCATGA CCTGACGTTG 
ATTATTCTAA TTGTTTGTGC TGTTGTTTCT CTAGTAGTTG GCCTTGCCAC TGAGGGGTGG 
CCGAAGGGCA TCTATGACGG CCTTGGGATA ATAACCAGCA TTTTATTGGT GGTGCTGGTT 
ACAGCATCCA GTGATTACAA ACAGTCACGG AAATTCATGG AGCTGGACTG TGAGAAAAAG 
AAGATATATG CCCTTGTCAC TAGAGATAGG AAAACCAAAA GGGTACTGAT TCATGATTTG 
GTTGTGGGTG ACATCTTGCA TCTTTCAATA GGTGATGTGG TTCCTGCAGA TGGCCTGTTC 
ATCTCTGGCT ACTGCTTAGT CATAGATGAA TCTAGTTTAT CAGGTGAAAG TGAACCAGTT 
CATGTTTTTG AAGAAAAGCC TTTCATTCAT GCTGGAAGTA AAGTGGTAGA TGGGACTGCC 
AAGATGCTTG TAACTGCTGT TGGTATGCGT ACTGAATGGG GCAAAATCAT GGATACTCTG 
AATGATGATG GCGTGGATGA AACCCCTTTA CAAGTTAAGC TTAATGGTGT GGCAACAATC 
ATTGGACAGA TCGGGTTGGT GTTTGCTATC CTCACATTTT TGGTACTTCT AGTGAGGTTC 
CTTGTTGACA AGGGGAAGGA CGTTGGGTTG TTAAATTGGT CAGCAAATGA TGCACTGACA 
ATAGTGAACT ACTTTGCTAT TGCAGTGACA ATCATTGTTG TCGCAGTCCC TGAGGGTCTA 
CCACTGGCTG TCACTCTTAG CCTTGCGTTT GCCATGCAAA AGTTGATGAA TGATAAAGCA 
CTAGTGAGGC ATCTCGCTGC ATGTGAAACC ATGGGTTCAG CTAGTTGTAT TTGCACTGAT 
AAGACTGGAA CTTTGACGAC CAACCACATG ATCGTTGACA AGGTTTGGAT CGGTGATGTA 
TCCAAGTCTG TCAATGGTGA TACAAATATG AATGAGCTGA AGGCTGCTAC TGCCGAAAGT 
GCTGTGGACA TACTTATTCA AGGAATATTT GTGAACACTG GTTCTGAGAT CGTGAAGGGA 
GACGATGGCA AAAAAACTAT CTTAGGCACA CCAACTGAAG CAGCATTATT GGAGTTTGGC 
TTGATCTTGC AAGGAGACCT GTATGGTGAG TACAATAAGT TGGCAAGAGT CAAAGTAGAG 
CCTTTTAACT CTGTTAAGAA AAAGATGTCT GTGCTAGTAC AGTTACCTAA TGGAGGCCTC 
CGGTCCTTCT GTAAAGGTGC ATCGGAACTT ATTTTAGGGC AGTGTGATAC TTTCCTCAAT 
AGTGAAGGAA ATTTAGCACC ACTGTCAGAA ATGCAAAAGC AAAATGTCTT AAATATAATA 
AACTCATTTG CTTCTGAGGC ATTAAGAACG CTTTGTATTG CATTTAAGGA TCTGAGTGAA 
ATTCCTGATG ACCAGACAAT ACCAGAGGAT GGTTACACAC TAATAGCACT TTTTGGCATA 
AAGGATCCAG TCCGTCCCGG TGTAAGGGAT GCAGTAATGA CCTGCATGGC TGCTGGAATC 
AAAGTAAAAA TGGTGACTGG AGACAACATC AATACTGCTA AAGCCATTGC CAAGGAATGT 
GGAATATTGA CCGAGGATGG AATAGCAATC GAAGGAAGAG AGCTTCACGA TAAGAGCGCA 
GATGAATTGA AGGAGATCCT GCCTAAAATT CAGGTAATGG CCCGGTCCTT GCCGATGGAC 
AAATATAAAT TGGTGACAAG CCTAAAAAGT ATGTATCAAG AGGTCGTGGC AGTTACTGGT 
GATGGAACAA ACGATGCTCC AGCATTGCGT GAGTCAGACA TAGGTCTGGC CATGGGCATT 
GCCGGCACTG AGGTTGCGAA AGAAAATGCC GATGTTATAA TAATGGATGA TAATTTCAGT 
ACCATTGTAA ATGTTGCTAG ATGGGGTCGT GCGGTTTACT TGAACATTCA GAAGTTTGTG 
CAGTTCCAGC TTACAGTTAA TATAGTGGCT CTGATAGTGA ATTTCATCTC AGCGTGTATC 
ATAGGTACTG CACCACTTAC TGCCGTTCAG TTGCTATGGG TGAACATGAT CATGGATACA 
TTAGGAGCCT TGGCCTTAGC GACAGAGCCA CCTAATGATG AAATGATGAA GAGACCACCT 
GTGAGACGGG GGCATGGTTT CATCACTCAG GTCATGTGGA GAAATATTCT TGGGCAGGCT 
TTATACCAGC TCCTTGTACT TGGTACTCTC ATGTTTGTTG GTAAGAGGAT CCTTAATATC 
GAAGGCCCAA ATGCTGATAT AACGATCAAT ACCCTCATAT TCAACTCTTT TGTGTTTTGC 
CAGGTTTTCA ACGAGATAAA TAGCAGGGAA ATGGAAAAGA TCAACGTGTT CAGAGGGATA 
CTGAAAAATT GGATCTTTAT CAGTATATTG ACAGCAACCG TCGTATTCCA AGTAATTATA 
GTGGAGTTTC TTGGAACCTT TGCAAACACC ATACCGCTGA GTTGGAAGCT GTGGTTGCTC 
AGTATTATTC TTGGCTCGGT CAGTATGGTC ATCTCTGTTA TCGTCAAGTG CATTCCCGTC 
GAATCTAGAA AAACAAATAT TAAGCCCCAT GGCTATGAGT TGATTCCTGA AGCCCCAGAA 
ACAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sorghum bicolor genome and the diversification of grasses.
Nature457(7229)551-6(2009 Jan)
Paterson AH,Bowers JE,Bruggmann R,Dubchak I,Grimwood J,Gundlach H,Haberer G,Hellsten U,Mitros T,Poliakov A,Schmutz J,Spannagl M,Tang H,Wang X,Wicker T,Bharti AK,Chapman J,Feltus FA,Gowik U,Grigoriev IV,Lyons E,Maher CA,Martis M,Narechania A,Otillar RP,Penning BW,Salamov AA,Wang Y,Zhang L,Carpita NC,Freeling M,Gingle AR,Hash CT,Keller B,Klein P,Kresovich S,McCann MC,Ming R,Peterson DG,Mehboob-ur-Rahman,Ware D,Westhoff P,Mayer KF,Messing J,Rokhsar DS

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LOC8064184 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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