SORBIDRAFT_09G027620 cDNA ORF clone, Sorghum bicolor(Sorghum)

Gene
Clones
gRNAs

Summary

Gene Symbol	SORBIDRAFT_09G027620
Entrez Gene ID	8061118
Full Name	zinc finger protein BRUTUS
Gene Type	protein-coding
Organism	Sorghum bicolor(Sorghum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002440153.2	XP_002440198.2	zinc finger protein BRUTUS
XM_002440153.1	XP_002440198.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Sorghum bicolor genome and the diversification of grasses.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KF, Messing J, Rokhsar DS
Nature457(7229)551-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SORBIDRAFT_09G027620 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SORBIDRAFT_09G027620 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc04489	XM_002440153.1 Latest version!	Sorghum bicolor hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSc105036	XM_002440153.2 Latest version!	Sorghum bicolor zinc finger protein BRUTUS (LOC8061118), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SORBIDRAFT_09G027620 ( XM_002440153.1 ) cDNA ORF clone, Sorghum bicolor(Sorghum) -> XP_002440198.1 Sorghum bicolor hypothetical protein, mRNA.

Price & Availability↑

CloneID	OSc04489
Clone ID Related Accession (Same CDS sequence)	XM_002440153.1
Accession Version	XM_002440153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1247328000000
Organism	Sorghum bicolor(Sorghum)
Product	hypothetical protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012878). COMPLETENESS: incomplete on the 5' end.

ATGCAGAAAA TTGAGAGTCA CTTCAATGAT GAGGAAATAA AGGTACTTCC TAAAGCTAGG 
ATAAAGTTCT CACCAGAGAA ACAGAGGGAA CTTATGTATC AGAGTCTATG TGTCATGCCA 
CTAAAGCTGT TGGAACGTGT TCTACCATGG TTTGTGGTGA AGCTGAATGA AGCAGAGGCA 
GTATCTTTTC TTCAGAATAT GCGATTAGCA GCACCTTCCT CTGAAACTGC ATTGGTTACC 
CTTCTCTCCG GCTGGGCATG CAAAGGTCGT CTGGAGGACA CATCCAACCC TGGAAAGTTC 
ATATGCACAT CAGGAGCGGT GGGCTGTGCA TCAGATGGAA ATGGTTCCGA AACATGCCAG 
TCATTCTGTC CATGTTATGT AAGTAGCAAT GGAGCTTTTT CGAAACCAGT TAAGCGAGCA 
AGTCATGGAG AATCTAGCAC TAACATTAAT AGAAGTCACT GCTCACAAGA TACGGAAGCC 
TCGCCGTGTA ACAACAGACC CTGTTACATT CCCAGGTTAA GAGTAGAAAG TAGCTACCTT 
GGTGTTAATT CATCTACCCC TGCAAAATCC TTTCGCTCTC TGCCTTACAA TAATTCTGCA 
CCTTCATTAT ATTCAAGCCT TTTTTCATGG GAGACAGATG CACTATCATC TGGCCCAGAT 
AACATTTCTA AGCCAATTGA TACCATATTC AAATTCCACA AGGCAATTCG TAAGGATTTG 
GAGTTTTTAG ATGTGGAATC TGGAAAGCTT ATTGACGGGA ATGAATCTTG CCTTCGCCAA 
TTTATCGGAA GGTTTCGTTT ACTGTGGGGT CTATATAGAG CACACAGCAA TGCTGAAGAT 
GAGATTGTAT TTCCTGCTCT TGAATCGAAG GAGACATTGC ACAATGTTAG TCATTCATAC 
ACTCTTGATC ACAAGCAGGA AGAAGAATTG TTCCAAGATA TATCCATTGT CCTATCTGAA 
CTTTCTCAAC TACATGATGG TTTGAGCCAT CCTCTTGATG TTGAAGCTGG AACAAATGAT 
ATTTCAAATA ATGAGATTGA TTGGGCTAAA AAGCGTAATG AACTTTTGAC AAAGCTTCAA 
GGATTATGCA AGTCTATCCA TGTCACATTG TCAAATCACG TTCACAGAGA AGAACTTGAA 
TTGTGGCCAT TGTTCGATAA ACATTTTTCT GTAGATGAGC AGGATAAGAT TATTGGACGT 
ATAATTGGAA CTACTGGTGC TGAGGTTCTG CAGTCAATGT TACCTTGGGT TACATCAGCA 
CTTAGTCTAG AGGAACAGAA CATGATGCTG GATACATGGA AGCAAGCAAC AAAAAATACA 
ATGTTCGATG AATGGCTAAA TGAATGGTGG AAAGGACCTT CAACTTCATC TGACCCCTCA 
GACAAGGCTT CTACTCTTTC AGAAGAAAAT GATTTTCAGG AGAATTTTGA ACAAAATGAT 
CAGATGTTTA GGCCTGGTTG GAAAGACATC TTCCGAATGA ACCAGAGTGA TCTCGAGGCT 
GAGATTCGAA AGGTCTCTCG AGATTCTACT CTTGATCCAA GGAGGAAGGC CTATCTGATA 
CAAAATCTCA TGACCAGCCG TTGGATAGCT GCTCAGCAGA AATCACCACA ACCAAGCGTC 
GAAGGACATA ATGGATGTAC ACGACGACCT GGTTGTGTTC CTTCATACCG AGACCCAGAG 
AAACAAATAT TTGGCTGTGA GCATTACAAA CGAAACTGCA AGCTTGTTGC TGCGTGCTGC 
AATAAGCTAT TCACATGCAG ATTCTGTCAT GATAAAGTTA GTGACCATAC AATGGACAGG 
AAGTCAGTGG TGGAGATGAT GTGCATGCTA TGTCTGAATG TTCAACCGGT CGGTCCAAAT 
TGCCAATCCC CTTCTTGCAA TGGACTGTCC ATGGCAAAGT ATTATTGTAG CATATGCAAG 
TTCTTCGATG ATGAAAGGAG TGTCTACCAC TGTCCTTTTT GCAACTTGTG TCGTCTTGGG 
AGTGGATTGG GCACTGATTT TTTTCATTGC ATGAAGTGCA ATTGTTGCCT TGGCATAAAG 
ATGATAGAAC ACAAATGCCG GGAAAAGATG CTAGAGATGA ACTGTCCGAT CTGCTGCGAC 
TTTTTATTTA CATCGAGTGC ACCAGTTAAA GGTCTTCCTT GTGGTCATTT CATGCATTCA 
GCTTGCTTTC AGGCATATAC TTGTACCCAC TACACTTGTC CAATCTGCTG CAAATCCTTG 
GGAGATATGA CGGTGTATTT TGGTATGCTT GATGGTTTGC TGGCTGCAGA ACAGCTTCCT 
GAGGAATACC GGGACCGATG TCAGGATATA CTTTGTAACG ACTGTGAGAG AAAAGGTAGG 
TCACGATTCC ATTGGCTGTA CCACAAATGT GGCTCCTGTG GTTCTTACAA CACCAGAGTT 
ATCAAGACTG ATACGGCAGA GTGTTCTACA TCAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002440198.1
CDS	1..2439
Translation	MQKIESHFNDEEIKVLPKARIKFSPEKQRELMYQSLCVMPLKLLERVLPWFVVKLNEAEAVSFLQNMRLAAPSSETALVTLLSGWACKGRLEDTSNPGKFICTSGAVGCASDGNGSETCQSFCPCYVSSNGAFSKPVKRASHGESSTNINRSHCSQDTEASPCNNRPCYIPRLRVESSYLGVNSSTPAKSFRSLPYNNSAPSLYSSLFSWETDALSSGPDNISKPIDTIFKFHKAIRKDLEFLDVESGKLIDGNESCLRQFIGRFRLLWGLYRAHSNAEDEIVFPALESKETLHNVSHSYTLDHKQEEELFQDISIVLSELSQLHDGLSHPLDVEAGTNDISNNEIDWAKKRNELLTKLQGLCKSIHVTLSNHVHREELELWPLFDKHFSVDEQDKIIGRIIGTTGAEVLQSMLPWVTSALSLEEQNMMLDTWKQATKNTMFDEWLNEWWKGPSTSSDPSDKASTLSEENDFQENFEQNDQMFRPGWKDIFRMNQSDLEAEIRKVSRDSTLDPRRKAYLIQNLMTSRWIAAQQKSPQPSVEGHNGCTRRPGCVPSYRDPEKQIFGCEHYKRNCKLVAACCNKLFTCRFCHDKVSDHTMDRKSVVEMMCMLCLNVQPVGPNCQSPSCNGLSMAKYYCSICKFFDDERSVYHCPFCNLCRLGSGLGTDFFHCMKCNCCLGIKMIEHKCREKMLEMNCPICCDFLFTSSAPVKGLPCGHFMHSACFQAYTCTHYTCPICCKSLGDMTVYFGMLDGLLAAEQLPEEYRDRCQDILCNDCERKGRSRFHWLYHKCGSCGSYNTRVIKTDTAECSTSN

Target ORF information:

RefSeq Version	XM_002440153.1
Organism	Sorghum bicolor(Sorghum)
Definition	Sorghum bicolor hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002440153.1

ORF Insert Sequence:

ATGCAGAAAA TTGAGAGTCA CTTCAATGAT GAGGAAATAA AGGTACTTCC TAAAGCTAGG 
ATAAAGTTCT CACCAGAGAA ACAGAGGGAA CTTATGTATC AGAGTCTATG TGTCATGCCA 
CTAAAGCTGT TGGAACGTGT TCTACCATGG TTTGTGGTGA AGCTGAATGA AGCAGAGGCA 
GTATCTTTTC TTCAGAATAT GCGATTAGCA GCACCTTCCT CTGAAACTGC ATTGGTTACC 
CTTCTCTCCG GCTGGGCATG CAAAGGTCGT CTGGAGGACA CATCCAACCC TGGAAAGTTC 
ATATGCACAT CAGGAGCGGT GGGCTGTGCA TCAGATGGAA ATGGTTCCGA AACATGCCAG 
TCATTCTGTC CATGTTATGT AAGTAGCAAT GGAGCTTTTT CGAAACCAGT TAAGCGAGCA 
AGTCATGGAG AATCTAGCAC TAACATTAAT AGAAGTCACT GCTCACAAGA TACGGAAGCC 
TCGCCGTGTA ACAACAGACC CTGTTACATT CCCAGGTTAA GAGTAGAAAG TAGCTACCTT 
GGTGTTAATT CATCTACCCC TGCAAAATCC TTTCGCTCTC TGCCTTACAA TAATTCTGCA 
CCTTCATTAT ATTCAAGCCT TTTTTCATGG GAGACAGATG CACTATCATC TGGCCCAGAT 
AACATTTCTA AGCCAATTGA TACCATATTC AAATTCCACA AGGCAATTCG TAAGGATTTG 
GAGTTTTTAG ATGTGGAATC TGGAAAGCTT ATTGACGGGA ATGAATCTTG CCTTCGCCAA 
TTTATCGGAA GGTTTCGTTT ACTGTGGGGT CTATATAGAG CACACAGCAA TGCTGAAGAT 
GAGATTGTAT TTCCTGCTCT TGAATCGAAG GAGACATTGC ACAATGTTAG TCATTCATAC 
ACTCTTGATC ACAAGCAGGA AGAAGAATTG TTCCAAGATA TATCCATTGT CCTATCTGAA 
CTTTCTCAAC TACATGATGG TTTGAGCCAT CCTCTTGATG TTGAAGCTGG AACAAATGAT 
ATTTCAAATA ATGAGATTGA TTGGGCTAAA AAGCGTAATG AACTTTTGAC AAAGCTTCAA 
GGATTATGCA AGTCTATCCA TGTCACATTG TCAAATCACG TTCACAGAGA AGAACTTGAA 
TTGTGGCCAT TGTTCGATAA ACATTTTTCT GTAGATGAGC AGGATAAGAT TATTGGACGT 
ATAATTGGAA CTACTGGTGC TGAGGTTCTG CAGTCAATGT TACCTTGGGT TACATCAGCA 
CTTAGTCTAG AGGAACAGAA CATGATGCTG GATACATGGA AGCAAGCAAC AAAAAATACA 
ATGTTCGATG AATGGCTAAA TGAATGGTGG AAAGGACCTT CAACTTCATC TGACCCCTCA 
GACAAGGCTT CTACTCTTTC AGAAGAAAAT GATTTTCAGG AGAATTTTGA ACAAAATGAT 
CAGATGTTTA GGCCTGGTTG GAAAGACATC TTCCGAATGA ACCAGAGTGA TCTCGAGGCT 
GAGATTCGAA AGGTCTCTCG AGATTCTACT CTTGATCCAA GGAGGAAGGC CTATCTGATA 
CAAAATCTCA TGACCAGCCG TTGGATAGCT GCTCAGCAGA AATCACCACA ACCAAGCGTC 
GAAGGACATA ATGGATGTAC ACGACGACCT GGTTGTGTTC CTTCATACCG AGACCCAGAG 
AAACAAATAT TTGGCTGTGA GCATTACAAA CGAAACTGCA AGCTTGTTGC TGCGTGCTGC 
AATAAGCTAT TCACATGCAG ATTCTGTCAT GATAAAGTTA GTGACCATAC AATGGACAGG 
AAGTCAGTGG TGGAGATGAT GTGCATGCTA TGTCTGAATG TTCAACCGGT CGGTCCAAAT 
TGCCAATCCC CTTCTTGCAA TGGACTGTCC ATGGCAAAGT ATTATTGTAG CATATGCAAG 
TTCTTCGATG ATGAAAGGAG TGTCTACCAC TGTCCTTTTT GCAACTTGTG TCGTCTTGGG 
AGTGGATTGG GCACTGATTT TTTTCATTGC ATGAAGTGCA ATTGTTGCCT TGGCATAAAG 
ATGATAGAAC ACAAATGCCG GGAAAAGATG CTAGAGATGA ACTGTCCGAT CTGCTGCGAC 
TTTTTATTTA CATCGAGTGC ACCAGTTAAA GGTCTTCCTT GTGGTCATTT CATGCATTCA 
GCTTGCTTTC AGGCATATAC TTGTACCCAC TACACTTGTC CAATCTGCTG CAAATCCTTG 
GGAGATATGA CGGTGTATTT TGGTATGCTT GATGGTTTGC TGGCTGCAGA ACAGCTTCCT 
GAGGAATACC GGGACCGATG TCAGGATATA CTTTGTAACG ACTGTGAGAG AAAAGGTAGG 
TCACGATTCC ATTGGCTGTA CCACAAATGT GGCTCCTGTG GTTCTTACAA CACCAGAGTT 
ATCAAGACTG ATACGGCAGA GTGTTCTACA TCAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SORBIDRAFT_09G027620 ( XM_002440153.2 ) cDNA ORF clone, Sorghum bicolor(Sorghum) -> XP_002440198.2 Sorghum bicolor zinc finger protein BRUTUS (LOC8061118), mRNA.

Price & Availability↑

CloneID	OSc105036
Clone ID Related Accession (Same CDS sequence)	XM_002440153.2
Accession Version	XM_002440153.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	zinc finger protein BRUTUS
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012878.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 13, 2017 this sequence version replaced XM_002440153.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC CGATGGCCGG AGAGGGTCCG ATCGCGGCGG CGATCCCGCG GTCCCCACCC 
CCTCTGTCCG AGGGCGGCGC GGGAGGGTCG GCGGCCGAGG CGCCCGTGCT CATATTCGTC 
TACTTCCACA AGGCGATCCG CGCGGAGCTG GAGCGGATGC ACGCCGCGGC GGTGCGGCTC 
GCCACGGGGT GCGCGGGCGG GGACGTGGCC GCGCTCGAGG CCCGCTGCCG GTTCCTCTTC 
ACCGTCTACA GGCACCACTG CGACGCCGAG GACGCGGTTA TTTTTCCAGC ACTTGATATT 
CGAGTGAAAA ATGTCGCAGG GACATATTCT CTTGAACACA AAGGAGAAAA TGATCTCTTC 
GCACATCTAT TCACTCTGCT GCAGCTGGAT GTGCAGAATG ATGATGCTAT TCGGAGGGAA 
CTTGCATCCT GTACTGGAGC AATTCGGACG TTTGTAACCC AACACATGTC CAAGGAAGAA 
GAACAGGTCT TCCCGTTGCT CATCAAAAAG TTTTCACACG AAGAGCAAGC CGATTTAGTC 
TGGCAGTTTT TATGTAGCAT CCCTGTAAAT ATGATGACAA ACTTCCTTCC GTGGATTTCT 
GCTTCTGTTT CAACAGATGA GAATCAAGAC ATTCTTGACT GCCTAAGTAA AATAGTTCCA 
GAAGAAAAAC TCCTCCAAGA GATTGTTTTC ACTTGGTTCG GAGGGAAATC ATTCAGAACA 
ATAACACAAA ATGTCAGTGA TCCTTGTTCA GAAAGCAGTT CTACATGTGA GTCTAGCTCT 
GGTCGATCAG ATAAACACGT ATGTTCACTT GAACACTCCA AAATTGGAAA AAGGAAGTCC 
ACAGAATCTA GTGAGTTTGT CACACATCCA ATTGATGAAA TTCTGTATTG GCACAATGCT 
ATTCGGAGAG AACTGAGTGA TATAGCAAAA GAGACGAAAA GGATACAGCA GTCTGGAGAC 
TTTTCTGACA TAGCAGGCTT TAATATGAGG CTGCAATTTA TTGCAGATGT GTGCATTTTC 
CACAGTATTG CTGAGGATCA AGTTATATTC CCTGCAGTGG ATGGTGAATT ATCTTTTGTA 
CAGGAGCATG CTGAAGAAGA ACAGCGGTTA AATGAGTTTA GGAGTTTAAT TGAACAAATT 
CAAATAGCAG GAGCCAAGTC AACTGTAATT GATTTTCATT CTGAGTTATG TTCACAAGCT 
GACGAAATAA TGCAGAAAAT TGAGAGTCAC TTCAATGATG AGGAAATAAA GGTACTTCCT 
AAAGCTAGGA TAAAGTTCTC ACCAGAGAAA CAGAGGGAAC TTATGTATCA GAGTCTATGT 
GTCATGCCAC TAAAGCTGTT GGAACGTGTT CTACCATGGT TTGTGGTGAA GCTGAATGAA 
GCAGAGGCAG TATCTTTTCT TCAGAATATG CGATTAGCAG CACCTTCCTC TGAAACTGCA 
TTGGTTACCC TTCTCTCCGG CTGGGCATGC AAAGGTCGTC TGGAGGACAC ATCCAACCCT 
GGAAAGTTCA TATGCACATC AGGAGCGGTG GGCTGTGCAT CAGATGGAAA TGGTTCCGAA 
ACATGCCAGT CATTCTGTCC ATGTTATGTA AGTAGCAATG GAGCTTTTTC GAAACCAGTT 
AAGCGAGCAA GTCATGGAGA ATCTAGCACT AACATTAATA GAAGTCACTG CTCACAAGAT 
ACGGAAGCCT CGCCGTGTAA CAACAGACCC TGTTACATTC CCAGGTTAAG AGTAGAAAGT 
AGCTACCTTG GTGTTAATTC ATCTACCCCT GCAAAATCCT TTCGCTCTCT GCCTTACAAT 
AATTCTGCAC CTTCATTATA TTCAAGCCTT TTTTCATGGG AGACAGATGC ACTATCATCT 
GGCCCAGATA ACATTTCTAA GCCAATTGAT ACCATATTCA AATTCCACAA GGCAATTCGT 
AAGGATTTGG AGTTTTTAGA TGTGGAATCT GGAAAGCTTA TTGACGGGAA TGAATCTTGC 
CTTCGCCAAT TTATCGGAAG GTTTCGTTTA CTGTGGGGTC TATATAGAGC ACACAGCAAT 
GCTGAAGATG AGATTGTATT TCCTGCTCTT GAATCGAAGG AGACATTGCA CAATGTTAGT 
CATTCATACA CTCTTGATCA CAAGCAGGAA GAAGAATTGT TCCAAGATAT ATCCATTGTC 
CTATCTGAAC TTTCTCAACT ACATGATGGT TTGAGCCATC CTCTTGATGT TGAAGCTGGA 
ACAAATGATA TTTCAAATAA TGAGATTGAT TGGGCTAAAA AGCGTAATGA ACTTTTGACA 
AAGCTTCAAG GATTATGCAA GTCTATCCAT GTCACATTGT CAAATCACGT TCACAGAGAA 
GAACTTGAAT TGTGGCCATT GTTCGATAAA CATTTTTCTG TAGATGAGCA GGATAAGATT 
ATTGGACGTA TAATTGGAAC TACTGGTGCT GAGGTTCTGC AGTCAATGTT ACCTTGGGTT 
ACATCAGCAC TTAGTCTAGA GGAACAGAAC ATGATGCTGG ATACATGGAA GCAAGCAACA 
AAAAATACAA TGTTCGATGA ATGGCTAAAT GAATGGTGGA AAGGACCTTC AACTTCATCT 
GACCCCTCAG ACAAGGCTTC TACTCTTTCA GAAGAAAATG ATTTTCAGGA GAATTTTGAA 
CAAAATGATC AGATGTTTAG GCCTGGTTGG AAAGACATCT TCCGAATGAA CCAGAGTGAT 
CTCGAGGCTG AGATTCGAAA GGTCTCTCGA GATTCTACTC TTGATCCAAG GAGGAAGGCC 
TATCTGATAC AAAATCTCAT GACCAGCCGT TGGATAGCTG CTCAGCAGAA ATCACCACAA 
CCAAGCGTCG AAGGACATAA TGGATGTACA CGACGACCTG GTTGTGTTCC TTCATACCGA 
GACCCAGAGA AACAAATATT TGGCTGTGAG CATTACAAAC GAAACTGCAA GCTTGTTGCT 
GCGTGCTGCA ATAAGCTATT CACATGCAGA TTCTGTCATG ATAAAGTTAG TGACCATACA 
ATGGACAGGA AGTCAGTGGT GGAGATGATG TGCATGCTAT GTCTGAATGT TCAACCGGTC 
GGTCCAAATT GCCAATCCCC TTCTTGCAAT GGACTGTCCA TGGCAAAGTA TTATTGTAGC 
ATATGCAAGT TCTTCGATGA TGAAAGGAGT GTCTACCACT GTCCTTTTTG CAACTTGTGT 
CGTCTTGGGA GTGGATTGGG CACTGATTTT TTTCATTGCA TGAAGTGCAA TTGTTGCCTT 
GGCATAAAGA TGATAGAACA CAAATGCCGG GAAAAGATGC TAGAGATGAA CTGTCCGATC 
TGCTGCGACT TTTTATTTAC ATCGAGTGCA CCAGTTAAAG GTCTTCCTTG TGGTCATTTC 
ATGCATTCAG CTTGCTTTCA GGCATATACT TGTACCCACT ACACTTGTCC AATCTGCTGC 
AAATCCTTGG GAGATATGAC GGTGTATTTT GGTATGCTTG ATGGTTTGCT GGCTGCAGAA 
CAGCTTCCTG AGGAATACCG GGACCGATGT CAGGATATAC TTTGTAACGA CTGTGAGAGA 
AAAGGTAGGT CACGATTCCA TTGGCTGTAC CACAAATGTG GCTCCTGTGG TTCTTACAAC 
ACCAGAGTTA TCAAGACTGA TACGGCAGAG TGTTCTACAT CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002440198.2
CDS	175..3822
Translation	MATPMAGEGPIAAAIPRSPPPLSEGGAGGSAAEAPVLIFVYFHKAIRAELERMHAAAVRLATGCAGGDVAALEARCRFLFTVYRHHCDAEDAVIFPALDIRVKNVAGTYSLEHKGENDLFAHLFTLLQLDVQNDDAIRRELASCTGAIRTFVTQHMSKEEEQVFPLLIKKFSHEEQADLVWQFLCSIPVNMMTNFLPWISASVSTDENQDILDCLSKIVPEEKLLQEIVFTWFGGKSFRTITQNVSDPCSESSSTCESSSGRSDKHVCSLEHSKIGKRKSTESSEFVTHPIDEILYWHNAIRRELSDIAKETKRIQQSGDFSDIAGFNMRLQFIADVCIFHSIAEDQVIFPAVDGELSFVQEHAEEEQRLNEFRSLIEQIQIAGAKSTVIDFHSELCSQADEIMQKIESHFNDEEIKVLPKARIKFSPEKQRELMYQSLCVMPLKLLERVLPWFVVKLNEAEAVSFLQNMRLAAPSSETALVTLLSGWACKGRLEDTSNPGKFICTSGAVGCASDGNGSETCQSFCPCYVSSNGAFSKPVKRASHGESSTNINRSHCSQDTEASPCNNRPCYIPRLRVESSYLGVNSSTPAKSFRSLPYNNSAPSLYSSLFSWETDALSSGPDNISKPIDTIFKFHKAIRKDLEFLDVESGKLIDGNESCLRQFIGRFRLLWGLYRAHSNAEDEIVFPALESKETLHNVSHSYTLDHKQEEELFQDISIVLSELSQLHDGLSHPLDVEAGTNDISNNEIDWAKKRNELLTKLQGLCKSIHVTLSNHVHREELELWPLFDKHFSVDEQDKIIGRIIGTTGAEVLQSMLPWVTSALSLEEQNMMLDTWKQATKNTMFDEWLNEWWKGPSTSSDPSDKASTLSEENDFQENFEQNDQMFRPGWKDIFRMNQSDLEAEIRKVSRDSTLDPRRKAYLIQNLMTSRWIAAQQKSPQPSVEGHNGCTRRPGCVPSYRDPEKQIFGCEHYKRNCKLVAACCNKLFTCRFCHDKVSDHTMDRKSVVEMMCMLCLNVQPVGPNCQSPSCNGLSMAKYYCSICKFFDDERSVYHCPFCNLCRLGSGLGTDFFHCMKCNCCLGIKMIEHKCREKMLEMNCPICCDFLFTSSAPVKGLPCGHFMHSACFQAYTCTHYTCPICCKSLGDMTVYFGMLDGLLAAEQLPEEYRDRCQDILCNDCERKGRSRFHWLYHKCGSCGSYNTRVIKTDTAECSTSN

Target ORF information:

RefSeq Version	XM_002440153.2
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor zinc finger protein BRUTUS (LOC8061118), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002440153.2

ORF Insert Sequence:

ATGGCGACGC CGATGGCCGG AGAGGGTCCG ATCGCGGCGG CGATCCCGCG GTCCCCACCC 
CCTCTGTCCG AGGGCGGCGC GGGAGGGTCG GCGGCCGAGG CGCCCGTGCT CATATTCGTC 
TACTTCCACA AGGCGATCCG CGCGGAGCTG GAGCGGATGC ACGCCGCGGC GGTGCGGCTC 
GCCACGGGGT GCGCGGGCGG GGACGTGGCC GCGCTCGAGG CCCGCTGCCG GTTCCTCTTC 
ACCGTCTACA GGCACCACTG CGACGCCGAG GACGCGGTTA TTTTTCCAGC ACTTGATATT 
CGAGTGAAAA ATGTCGCAGG GACATATTCT CTTGAACACA AAGGAGAAAA TGATCTCTTC 
GCACATCTAT TCACTCTGCT GCAGCTGGAT GTGCAGAATG ATGATGCTAT TCGGAGGGAA 
CTTGCATCCT GTACTGGAGC AATTCGGACG TTTGTAACCC AACACATGTC CAAGGAAGAA 
GAACAGGTCT TCCCGTTGCT CATCAAAAAG TTTTCACACG AAGAGCAAGC CGATTTAGTC 
TGGCAGTTTT TATGTAGCAT CCCTGTAAAT ATGATGACAA ACTTCCTTCC GTGGATTTCT 
GCTTCTGTTT CAACAGATGA GAATCAAGAC ATTCTTGACT GCCTAAGTAA AATAGTTCCA 
GAAGAAAAAC TCCTCCAAGA GATTGTTTTC ACTTGGTTCG GAGGGAAATC ATTCAGAACA 
ATAACACAAA ATGTCAGTGA TCCTTGTTCA GAAAGCAGTT CTACATGTGA GTCTAGCTCT 
GGTCGATCAG ATAAACACGT ATGTTCACTT GAACACTCCA AAATTGGAAA AAGGAAGTCC 
ACAGAATCTA GTGAGTTTGT CACACATCCA ATTGATGAAA TTCTGTATTG GCACAATGCT 
ATTCGGAGAG AACTGAGTGA TATAGCAAAA GAGACGAAAA GGATACAGCA GTCTGGAGAC 
TTTTCTGACA TAGCAGGCTT TAATATGAGG CTGCAATTTA TTGCAGATGT GTGCATTTTC 
CACAGTATTG CTGAGGATCA AGTTATATTC CCTGCAGTGG ATGGTGAATT ATCTTTTGTA 
CAGGAGCATG CTGAAGAAGA ACAGCGGTTA AATGAGTTTA GGAGTTTAAT TGAACAAATT 
CAAATAGCAG GAGCCAAGTC AACTGTAATT GATTTTCATT CTGAGTTATG TTCACAAGCT 
GACGAAATAA TGCAGAAAAT TGAGAGTCAC TTCAATGATG AGGAAATAAA GGTACTTCCT 
AAAGCTAGGA TAAAGTTCTC ACCAGAGAAA CAGAGGGAAC TTATGTATCA GAGTCTATGT 
GTCATGCCAC TAAAGCTGTT GGAACGTGTT CTACCATGGT TTGTGGTGAA GCTGAATGAA 
GCAGAGGCAG TATCTTTTCT TCAGAATATG CGATTAGCAG CACCTTCCTC TGAAACTGCA 
TTGGTTACCC TTCTCTCCGG CTGGGCATGC AAAGGTCGTC TGGAGGACAC ATCCAACCCT 
GGAAAGTTCA TATGCACATC AGGAGCGGTG GGCTGTGCAT CAGATGGAAA TGGTTCCGAA 
ACATGCCAGT CATTCTGTCC ATGTTATGTA AGTAGCAATG GAGCTTTTTC GAAACCAGTT 
AAGCGAGCAA GTCATGGAGA ATCTAGCACT AACATTAATA GAAGTCACTG CTCACAAGAT 
ACGGAAGCCT CGCCGTGTAA CAACAGACCC TGTTACATTC CCAGGTTAAG AGTAGAAAGT 
AGCTACCTTG GTGTTAATTC ATCTACCCCT GCAAAATCCT TTCGCTCTCT GCCTTACAAT 
AATTCTGCAC CTTCATTATA TTCAAGCCTT TTTTCATGGG AGACAGATGC ACTATCATCT 
GGCCCAGATA ACATTTCTAA GCCAATTGAT ACCATATTCA AATTCCACAA GGCAATTCGT 
AAGGATTTGG AGTTTTTAGA TGTGGAATCT GGAAAGCTTA TTGACGGGAA TGAATCTTGC 
CTTCGCCAAT TTATCGGAAG GTTTCGTTTA CTGTGGGGTC TATATAGAGC ACACAGCAAT 
GCTGAAGATG AGATTGTATT TCCTGCTCTT GAATCGAAGG AGACATTGCA CAATGTTAGT 
CATTCATACA CTCTTGATCA CAAGCAGGAA GAAGAATTGT TCCAAGATAT ATCCATTGTC 
CTATCTGAAC TTTCTCAACT ACATGATGGT TTGAGCCATC CTCTTGATGT TGAAGCTGGA 
ACAAATGATA TTTCAAATAA TGAGATTGAT TGGGCTAAAA AGCGTAATGA ACTTTTGACA 
AAGCTTCAAG GATTATGCAA GTCTATCCAT GTCACATTGT CAAATCACGT TCACAGAGAA 
GAACTTGAAT TGTGGCCATT GTTCGATAAA CATTTTTCTG TAGATGAGCA GGATAAGATT 
ATTGGACGTA TAATTGGAAC TACTGGTGCT GAGGTTCTGC AGTCAATGTT ACCTTGGGTT 
ACATCAGCAC TTAGTCTAGA GGAACAGAAC ATGATGCTGG ATACATGGAA GCAAGCAACA 
AAAAATACAA TGTTCGATGA ATGGCTAAAT GAATGGTGGA AAGGACCTTC AACTTCATCT 
GACCCCTCAG ACAAGGCTTC TACTCTTTCA GAAGAAAATG ATTTTCAGGA GAATTTTGAA 
CAAAATGATC AGATGTTTAG GCCTGGTTGG AAAGACATCT TCCGAATGAA CCAGAGTGAT 
CTCGAGGCTG AGATTCGAAA GGTCTCTCGA GATTCTACTC TTGATCCAAG GAGGAAGGCC 
TATCTGATAC AAAATCTCAT GACCAGCCGT TGGATAGCTG CTCAGCAGAA ATCACCACAA 
CCAAGCGTCG AAGGACATAA TGGATGTACA CGACGACCTG GTTGTGTTCC TTCATACCGA 
GACCCAGAGA AACAAATATT TGGCTGTGAG CATTACAAAC GAAACTGCAA GCTTGTTGCT 
GCGTGCTGCA ATAAGCTATT CACATGCAGA TTCTGTCATG ATAAAGTTAG TGACCATACA 
ATGGACAGGA AGTCAGTGGT GGAGATGATG TGCATGCTAT GTCTGAATGT TCAACCGGTC 
GGTCCAAATT GCCAATCCCC TTCTTGCAAT GGACTGTCCA TGGCAAAGTA TTATTGTAGC 
ATATGCAAGT TCTTCGATGA TGAAAGGAGT GTCTACCACT GTCCTTTTTG CAACTTGTGT 
CGTCTTGGGA GTGGATTGGG CACTGATTTT TTTCATTGCA TGAAGTGCAA TTGTTGCCTT 
GGCATAAAGA TGATAGAACA CAAATGCCGG GAAAAGATGC TAGAGATGAA CTGTCCGATC 
TGCTGCGACT TTTTATTTAC ATCGAGTGCA CCAGTTAAAG GTCTTCCTTG TGGTCATTTC 
ATGCATTCAG CTTGCTTTCA GGCATATACT TGTACCCACT ACACTTGTCC AATCTGCTGC 
AAATCCTTGG GAGATATGAC GGTGTATTTT GGTATGCTTG ATGGTTTGCT GGCTGCAGAA 
CAGCTTCCTG AGGAATACCG GGACCGATGT CAGGATATAC TTTGTAACGA CTGTGAGAGA 
AAAGGTAGGT CACGATTCCA TTGGCTGTAC CACAAATGTG GCTCCTGTGG TTCTTACAAC 
ACCAGAGTTA TCAAGACTGA TACGGCAGAG TGTTCTACAT CAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sorghum bicolor genome and the diversification of grasses.
Nature457(7229)551-6(2009 Jan)
Paterson AH,Bowers JE,Bruggmann R,Dubchak I,Grimwood J,Gundlach H,Haberer G,Hellsten U,Mitros T,Poliakov A,Schmutz J,Spannagl M,Tang H,Wang X,Wicker T,Bharti AK,Chapman J,Feltus FA,Gowik U,Grigoriev IV,Lyons E,Maher CA,Martis M,Narechania A,Otillar RP,Penning BW,Salamov AA,Wang Y,Zhang L,Carpita NC,Freeling M,Gingle AR,Hash CT,Keller B,Klein P,Kresovich S,McCann MC,Ming R,Peterson DG,Mehboob-ur-Rahman,Ware D,Westhoff P,Mayer KF,Messing J,Rokhsar DS

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SORBIDRAFT_09G027620 cDNA ORF clone, Sorghum bicolor(Sorghum)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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