LINJ_10_1030 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_10_1030
Entrez Gene ID	5067178
Full Name	putative putative eIF-2B GDP-GTP exchange factor
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001463712.1	XP_001463749.1	putative putative eIF-2B GDP-GTP exchange factor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_10_1030 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_10_1030 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc01081	XM_001463712.1 Latest version!	Leishmania infantum JPCM5 putative putative eIF-2B GDP-GTP exchange factor partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_10_1030 ( XM_001463712.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001463749.1 Leishmania infantum JPCM5 putative putative eIF-2B GDP-GTP exchange factor partial mRNA.

Price & Availability↑

CloneID	OLc01081
Clone ID Related Accession (Same CDS sequence)	XM_001463712.1
Accession Version	XM_001463712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative putative eIF-2B GDP-GTP exchange factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009394). COMPLETENESS: incomplete on both ends.

ATGCTGAGTA ATGTGCCAGA AGAGTCTGAG AAGCTGGTGC GAGAGGTCAG CGACCTCGCA 
GAGGCCTTCA TCACAAATCT GAAGCGCGGA AAGCTCGCCG CCGCGCGCAG TGGCGAGGAG 
AGTCTCTCCA CGGCGGCCGC AGTGGAGATG GCAGATGTAA TGTCGACCAT CGTGCGTCTC 
TTCCAGAATG CCGAGAAAGA CCCGCACAGC ACAACAATAC AGCTGCTTAA GGCCGGCTAC 
CCGGCGGCCG AGGTGGCGAG CATGCAGGTC AGCCGCCTTC TCATTCTCGT TGCTCGCACC 
GGTGATGCGA TGCTGCGAGC ACAGTTTGCC ACGCTCTTTC TCATGAACGT CACGCGTCGC 
GTGCTTTCCA TCGTGCGGGA GTCCGCCGCC AACAGCAAGG CCACCGACGC GGAGCTCGAA 
GATGAGGCCA AGACAATACT GAACGATCTC GCCTCGGATG ACGAGGCAGC GGTGGTTGAA 
ACCCCTGCCG ACACTAGTTC GAGCTCCAGC AACAGGTACG GCATGGACGA CTCCGCAGAT 
CCGGCCAACC CGTCCCTGTC AGCCAAGGGC ATGAAGCCGG CTCTCAAGAG GGATACTCTC 
GTCTCTCAGC CTCTCCCTCG CCGCGGACTC TCGGTGCGCT TCGCTGGCAC CTCCAACAGC 
TCCGAGGAGG CGAGCGGTGG CCACATGCGC CAGCTGCAGC AGCACAGCAC GTCGGTGGCT 
AGTGACTTTG GGGGGCCGCT GCTGCAGCAC ACCGGCTCGA TAGTGGCGAC GGAGATACCG 
GGCCGGCAGC TGCTGAAGCG CGCGCCGAGC CGCTGTGAGC AAGTGCTGGA GGCGGGCGGC 
GGGGAGATTG AGTTCCCGGT GCGCATGGAG ATCTTTTACC AGGACGCCCT GGACGCCATC 
GACGAGTTCA AGCGGGAGCT AGAGGGGATG ACCGAGGAGC TGTGTCGCCG TTCCACGCGG 
CAGCTGCACT CGTCGGACAC GATCATCACG CTGGGGTGCA GTCATACCAT CCGCCGCTAC 
CTGCTGGAAG CTGCTCAAGC GGGGCACCAC TTCAAAGTCT TCATCCTTGA GGGTGCACCG 
CTGCCGGCGC AGGCGACGCA GGAGTTTGCG GAGGCGCTGT GCAGTCGCGG CATCACTGCG 
CAGCTGTTGC CAGACAGCTC CGCCTACGTG GTCATGAGCA TGTGCACCAA GGTGCTGGTT 
GGTGCCGAGA ACGTCTTGGC CAACGGCGGC ATGCTCGCAT CCATCGGCAC CCACGTCCTC 
TGCGCCGCTG CTCGCCACTT TGCTGTCCCC GTGCTGGTCG CCACCACAAC GCTGAAGATG 
TCGCCGTACT ATCCAAGCGA CCAGCTGTGC ACGCGGCTGG TGCGCATTGC ACGGTCGGGG 
GCGCAGGAGA TGCCATGGTC GACCTACGGC TCCCCCGAGG ATGTGTGGCC CTCGCCGTTT 
GGCGTCTCCG TCTCCGACAG CGGCCGCCGC TTGACCATCC ACGCCCCCGT CACGGAGTAC 
GTGCCGCCCG AGCTCATTAC GTTGTTTTTA ACTAACGACA GCGAGCTGTT GCCCTCGCAG 
ATCCACCGCA TTGTGCGGGC GAACTACAGC GACGCAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001463749.1
CDS	1..1602
Translation	MLSNVPEESEKLVREVSDLAEAFITNLKRGKLAAARSGEESLSTAAAVEMADVMSTIVRLFQNAEKDPHSTTIQLLKAGYPAAEVASMQVSRLLILVARTGDAMLRAQFATLFLMNVTRRVLSIVRESAANSKATDAELEDEAKTILNDLASDDEAAVVETPADTSSSSSNRYGMDDSADPANPSLSAKGMKPALKRDTLVSQPLPRRGLSVRFAGTSNSSEEASGGHMRQLQQHSTSVASDFGGPLLQHTGSIVATEIPGRQLLKRAPSRCEQVLEAGGGEIEFPVRMEIFYQDALDAIDEFKRELEGMTEELCRRSTRQLHSSDTIITLGCSHTIRRYLLEAAQAGHHFKVFILEGAPLPAQATQEFAEALCSRGITAQLLPDSSAYVVMSMCTKVLVGAENVLANGGMLASIGTHVLCAAARHFAVPVLVATTTLKMSPYYPSDQLCTRLVRIARSGAQEMPWSTYGSPEDVWPSPFGVSVSDSGRRLTIHAPVTEYVPPELITLFLTNDSELLPSQIHRIVRANYSDAD

Target ORF information:

RefSeq Version	XM_001463712.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative putative eIF-2B GDP-GTP exchange factor partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001463712.1

ORF Insert Sequence:

ATGCTGAGTA ATGTGCCAGA AGAGTCTGAG AAGCTGGTGC GAGAGGTCAG CGACCTCGCA 
GAGGCCTTCA TCACAAATCT GAAGCGCGGA AAGCTCGCCG CCGCGCGCAG TGGCGAGGAG 
AGTCTCTCCA CGGCGGCCGC AGTGGAGATG GCAGATGTAA TGTCGACCAT CGTGCGTCTC 
TTCCAGAATG CCGAGAAAGA CCCGCACAGC ACAACAATAC AGCTGCTTAA GGCCGGCTAC 
CCGGCGGCCG AGGTGGCGAG CATGCAGGTC AGCCGCCTTC TCATTCTCGT TGCTCGCACC 
GGTGATGCGA TGCTGCGAGC ACAGTTTGCC ACGCTCTTTC TCATGAACGT CACGCGTCGC 
GTGCTTTCCA TCGTGCGGGA GTCCGCCGCC AACAGCAAGG CCACCGACGC GGAGCTCGAA 
GATGAGGCCA AGACAATACT GAACGATCTC GCCTCGGATG ACGAGGCAGC GGTGGTTGAA 
ACCCCTGCCG ACACTAGTTC GAGCTCCAGC AACAGGTACG GCATGGACGA CTCCGCAGAT 
CCGGCCAACC CGTCCCTGTC AGCCAAGGGC ATGAAGCCGG CTCTCAAGAG GGATACTCTC 
GTCTCTCAGC CTCTCCCTCG CCGCGGACTC TCGGTGCGCT TCGCTGGCAC CTCCAACAGC 
TCCGAGGAGG CGAGCGGTGG CCACATGCGC CAGCTGCAGC AGCACAGCAC GTCGGTGGCT 
AGTGACTTTG GGGGGCCGCT GCTGCAGCAC ACCGGCTCGA TAGTGGCGAC GGAGATACCG 
GGCCGGCAGC TGCTGAAGCG CGCGCCGAGC CGCTGTGAGC AAGTGCTGGA GGCGGGCGGC 
GGGGAGATTG AGTTCCCGGT GCGCATGGAG ATCTTTTACC AGGACGCCCT GGACGCCATC 
GACGAGTTCA AGCGGGAGCT AGAGGGGATG ACCGAGGAGC TGTGTCGCCG TTCCACGCGG 
CAGCTGCACT CGTCGGACAC GATCATCACG CTGGGGTGCA GTCATACCAT CCGCCGCTAC 
CTGCTGGAAG CTGCTCAAGC GGGGCACCAC TTCAAAGTCT TCATCCTTGA GGGTGCACCG 
CTGCCGGCGC AGGCGACGCA GGAGTTTGCG GAGGCGCTGT GCAGTCGCGG CATCACTGCG 
CAGCTGTTGC CAGACAGCTC CGCCTACGTG GTCATGAGCA TGTGCACCAA GGTGCTGGTT 
GGTGCCGAGA ACGTCTTGGC CAACGGCGGC ATGCTCGCAT CCATCGGCAC CCACGTCCTC 
TGCGCCGCTG CTCGCCACTT TGCTGTCCCC GTGCTGGTCG CCACCACAAC GCTGAAGATG 
TCGCCGTACT ATCCAAGCGA CCAGCTGTGC ACGCGGCTGG TGCGCATTGC ACGGTCGGGG 
GCGCAGGAGA TGCCATGGTC GACCTACGGC TCCCCCGAGG ATGTGTGGCC CTCGCCGTTT 
GGCGTCTCCG TCTCCGACAG CGGCCGCCGC TTGACCATCC ACGCCCCCGT CACGGAGTAC 
GTGCCGCCCG AGCTCATTAC GTTGTTTTTA ACTAACGACA GCGAGCTGTT GCCCTCGCAG 
ATCCACCGCA TTGTGCGGGC GAACTACAGC GACGCAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_10_1030 cDNA ORF clone, Leishmania infantum JPCM5

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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