LINJ_05_0590 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_05_0590
Entrez Gene ID	5066507
Full Name	putative ATP-dependent RNA helicase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001462985.1	XP_001463022.1	putative ATP-dependent RNA helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_05_0590 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_05_0590 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc00505	XM_001462985.1 Latest version!	Leishmania infantum JPCM5 putative ATP-dependent RNA helicase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_05_0590 ( XM_001462985.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001463022.1 Leishmania infantum JPCM5 putative ATP-dependent RNA helicase partial mRNA.

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CloneID	OLc00505
Clone ID Related Accession (Same CDS sequence)	XM_001462985.1
Accession Version	XM_001462985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative ATP-dependent RNA helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009390). COMPLETENESS: incomplete on both ends.

ATGCAGCCGC ATCCGTCATG GCGTGTCTAC GTCGGCGATG ACGTCGACGT TGGCGGCACT 
GGTGGTACTG CTGCCGCTGC TGCGGACCAG ACGAACACTC ACGCATCGCC CAACATGGAG 
GAGGATGTGG CGCGCTTGCT CGACTCGGCG TTCGCGCGGC GTGCCCGCTA TACCCTACAG 
ACCCTCCCAG CGAGGATTCG CCAGCAGCCA TCTGCCGCGC CGTCCCCCGT GCTGCGCTGG 
CTGCTGCAGC ACAGTGTGGA CGAAGGGCAG AGAAAGAAGG AGCAGGCAGG AACGGCGCCC 
GGTGCGACGG ACAAACCTAG TGCCACTCCT GCGTCTGCGC TGTCGCCCAC CGTTGCTGCA 
ACATCAGGAT GCGTTTCGGC AGCAGCAGCA GCGACGTCAG CGCTAGCGCG CCGCGGTGTC 
GGACCGAAGG ACCCGCTGGT TCAGCGCCTC CTGAAGAAGC AGCAGAAGAC AGCTTCGTCT 
GCGTCCGCAG CAACGTCAGC CACGTCCGCC GCGCCAAGCA GTGCCACGAA TGCCACCGCC 
TCAACCGTTA TCAGCGGCAG CACAACCGCG CCCATGTCGT GTGCCTCGCA CTGGACGCAA 
CGCCCCTTCT CTGCCATGAC GTCCACCAGC TGGAAGGTAT TCCGGCAGCA GCTGGGCATC 
ACCGTCGAGC TGGTGGCGCC TGCGCAGCCG CCCACAGAGA CCAGCGGCGC CAGCGCCGCA 
CAGCCAAGCA GTTTTGTCAA GACGTTGCCG CCAGCCGACA CGCTACCGGC GATCCGCTGC 
TGGGAGGAAG CCCAGCTGCC GCTTTCGCTT GGCCTGTGCG TCACGCACGC GTACGCGCTG 
CCGACGGCGG TGCAGGCGCA GTGCGTGCCG CTCGCACTTC TCCCGCGGCC CGCGCCTTTG 
GTCACGGGCA CGAACAGTGG CGGTGTGGCT GGTGCGGCTA CGGCGACGGT GCTTGGCATG 
GACATCCTCG CAGTAGCGGA GACCGGTAGC GGCAAGACCG CGGCGTACCT CGTGCCGCTG 
CTGCACCACG TGCTGTGCCG TGCACCGAAG CTGCTGGGCC ACCCCGACCG GATCTCACTG 
GGGCCGCTGA GCTTGGTGAT TGTGCCGACG CGTGAGCTGG CAGAACAGGT GACGGCGTCC 
TTTCTGCAGT TGTGCGGCCA GGCGCCGGCT GCCTCCATGT ACAACAGCGG CGGGGGCCAC 
ATCTCGCAGG ACACCATCCG CGCCTGGGAG ACGCACGAGT TGGATGTGAG TGGCGCCAGT 
GGTCACGATC GTGCGTCCTC TGTGGCCGCG GCGGGGGCGG CGCGAAACCA TCTCAGCGAG 
CTGCGCGTGG TCAAGGTGGT TGGTGGCGAG AATCGCGATG CTCAGTACGC CGCCCTGGCA 
CGCGGCGCGC ACTGCGTCGT CGGCACGGTG GGCCAGCTGC AAGTACTTCT CGAGGACCGC 
CTGCTCTCGC TCGGCAACAC GCAGTTCGTC GTGATGGACG AGGCAGATCG GATGATCGAT 
GAGCAGCAGG AGGAGCGGCT TACTGCAGTC CTAGAGCGGT GCCCGCAGCC GCGGCAGACA 
GTCATGTTCA CAGCGACGCT GAGCGTGGCC TGCGCGGCAG TGGCGAAGCG CTACCTCGCC 
AAGGCCGGCT ATTACCTGGT GCGCACACCG TACCGGTGCG CCTCGATCTG CCAGTCCTTC 
GAGCTGGTCG CCGACACCGG CGCCTCAGTC ACGGGGACTG CAGAGGGCCC CGTCCCCGCC 
GCGTCTGGCG CTGCCACAGC GACGGCAGAG GGGCGGCAAA AGAAGCCGTC TCGGCAACAA 
GGACACACCG AGAAGGGCGA GCCGCAACCG CCGCAGCGGC AGCGTCTGCT GCACCCTCTT 
ATACACGCCC AGAAGTTTGT GCGGTTGGTG GCGTGGCTCG TCTACGGCAC CGGCCCGATC 
ATCGTCTTCG CCAACGAAAA GTCGACGTGC GACGCGCTGT GGGAGGAGCT GCGTGCCGAG 
GCGGAGCACC TGGACGCACA GCAGGAATAC TACACAATCG AGGACATTGT CGGCCCGCCG 
CCGGACGGCT TGTCGTTTCG CGAAGACACC GCGGTTGGTC AAGGGGCAAC GTCGTCCAGC 
GTGGCGGCGC GGCGCACGCC GACGTTGGCG AACCTAACCT CCGTTGCCGT GGTGCACTCG 
GAGCTGTCGC AGACGGAGCG GCGCTCTCTC GTGGCGCAGT TCCAGCGGCG TCAGCGGCAC 
GTTCTCATCA CGACAGACCT TCTCGCTCGT GGCCTTGACG TGGCGGGGGT GACGCTTGTG 
ATCAACTACG ACGTGCCGTG GGCAGCCTCT GCCTCGCCGA GCGACGCTGT GACGCTCTAC 
ATCCACCGCA TCGGGCGTAC CGGCCGTGCA GGCTCCACCG GCGTCGCCGT AACTTTCGTG 
ACGTTGCCTG CCGCGATGGT ACAACGGGCC GAGGAGGTGG CGAGGGAGGA GGCGAGGGAC 
GCTGCGCCGA AGCCGCCTGA AGGCCGCACG AAACCGCCCA GCGCAGCACC CCGCGTGTAT 
GGCGACGAGG ATGACGACCT CGCCGCCCTC GGTGAGCTCT TCGACGGCGA CACGCGTAAT 
GTCTGTATGG CAGGGGCAGC GGCGCTATCC ACCACTGCCG CAGATAATGC CAGCGGCCGT 
CGTCGCCGCC GCGGTGACGA CGACAGCGAC GACACAGAAG TGGACAGCAG CAACGACAGC 
GATGACGACA ACGATGATGA GGACACCAAG AAGGAGGGCG CCACCGGAAA TACGAGCAAC 
ACCGCGAATC ACAACGGCAA TGGCATGCGG AAGCCGCCGA GGAAGCGCCG CAAGCGCTCG 
GTCGCCACCT TCGCCAGCGA CGTCGCGGTG CTGCGGCCCC TCTGGTCCTT CCTCGTCGAG 
TGTGCCGAGG GCGAGGGATG CCGCGATGGA GCGGCGGCGC TGCGGCGCGG CACATACGCA 
AAGATCAGTG TTCCGGCGGC GCTGGGGTGC ATCATGGCGC AGCTCTCTGC CACCTCCCAG 
TACGGCCGCA TTACCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001463022.1
CDS	1..3021
Translation	MQPHPSWRVYVGDDVDVGGTGGTAAAAADQTNTHASPNMEEDVARLLDSAFARRARYTLQTLPARIRQQPSAAPSPVLRWLLQHSVDEGQRKKEQAGTAPGATDKPSATPASALSPTVAATSGCVSAAAAATSALARRGVGPKDPLVQRLLKKQQKTASSASAATSATSAAPSSATNATASTVISGSTTAPMSCASHWTQRPFSAMTSTSWKVFRQQLGITVELVAPAQPPTETSGASAAQPSSFVKTLPPADTLPAIRCWEEAQLPLSLGLCVTHAYALPTAVQAQCVPLALLPRPAPLVTGTNSGGVAGAATATVLGMDILAVAETGSGKTAAYLVPLLHHVLCRAPKLLGHPDRISLGPLSLVIVPTRELAEQVTASFLQLCGQAPAASMYNSGGGHISQDTIRAWETHELDVSGASGHDRASSVAAAGAARNHLSELRVVKVVGGENRDAQYAALARGAHCVVGTVGQLQVLLEDRLLSLGNTQFVVMDEADRMIDEQQEERLTAVLERCPQPRQTVMFTATLSVACAAVAKRYLAKAGYYLVRTPYRCASICQSFELVADTGASVTGTAEGPVPAASGAATATAEGRQKKPSRQQGHTEKGEPQPPQRQRLLHPLIHAQKFVRLVAWLVYGTGPIIVFANEKSTCDALWEELRAEAEHLDAQQEYYTIEDIVGPPPDGLSFREDTAVGQGATSSSVAARRTPTLANLTSVAVVHSELSQTERRSLVAQFQRRQRHVLITTDLLARGLDVAGVTLVINYDVPWAASASPSDAVTLYIHRIGRTGRAGSTGVAVTFVTLPAAMVQRAEEVAREEARDAAPKPPEGRTKPPSAAPRVYGDEDDDLAALGELFDGDTRNVCMAGAAALSTTAADNASGRRRRRGDDDSDDTEVDSSNDSDDDNDDEDTKKEGATGNTSNTANHNGNGMRKPPRKRRKRSVATFASDVAVLRPLWSFLVECAEGEGCRDGAAALRRGTYAKISVPAALGCIMAQLSATSQYGRITL

Target ORF information:

RefSeq Version	XM_001462985.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative ATP-dependent RNA helicase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001462985.1

ORF Insert Sequence:

ATGCAGCCGC ATCCGTCATG GCGTGTCTAC GTCGGCGATG ACGTCGACGT TGGCGGCACT 
GGTGGTACTG CTGCCGCTGC TGCGGACCAG ACGAACACTC ACGCATCGCC CAACATGGAG 
GAGGATGTGG CGCGCTTGCT CGACTCGGCG TTCGCGCGGC GTGCCCGCTA TACCCTACAG 
ACCCTCCCAG CGAGGATTCG CCAGCAGCCA TCTGCCGCGC CGTCCCCCGT GCTGCGCTGG 
CTGCTGCAGC ACAGTGTGGA CGAAGGGCAG AGAAAGAAGG AGCAGGCAGG AACGGCGCCC 
GGTGCGACGG ACAAACCTAG TGCCACTCCT GCGTCTGCGC TGTCGCCCAC CGTTGCTGCA 
ACATCAGGAT GCGTTTCGGC AGCAGCAGCA GCGACGTCAG CGCTAGCGCG CCGCGGTGTC 
GGACCGAAGG ACCCGCTGGT TCAGCGCCTC CTGAAGAAGC AGCAGAAGAC AGCTTCGTCT 
GCGTCCGCAG CAACGTCAGC CACGTCCGCC GCGCCAAGCA GTGCCACGAA TGCCACCGCC 
TCAACCGTTA TCAGCGGCAG CACAACCGCG CCCATGTCGT GTGCCTCGCA CTGGACGCAA 
CGCCCCTTCT CTGCCATGAC GTCCACCAGC TGGAAGGTAT TCCGGCAGCA GCTGGGCATC 
ACCGTCGAGC TGGTGGCGCC TGCGCAGCCG CCCACAGAGA CCAGCGGCGC CAGCGCCGCA 
CAGCCAAGCA GTTTTGTCAA GACGTTGCCG CCAGCCGACA CGCTACCGGC GATCCGCTGC 
TGGGAGGAAG CCCAGCTGCC GCTTTCGCTT GGCCTGTGCG TCACGCACGC GTACGCGCTG 
CCGACGGCGG TGCAGGCGCA GTGCGTGCCG CTCGCACTTC TCCCGCGGCC CGCGCCTTTG 
GTCACGGGCA CGAACAGTGG CGGTGTGGCT GGTGCGGCTA CGGCGACGGT GCTTGGCATG 
GACATCCTCG CAGTAGCGGA GACCGGTAGC GGCAAGACCG CGGCGTACCT CGTGCCGCTG 
CTGCACCACG TGCTGTGCCG TGCACCGAAG CTGCTGGGCC ACCCCGACCG GATCTCACTG 
GGGCCGCTGA GCTTGGTGAT TGTGCCGACG CGTGAGCTGG CAGAACAGGT GACGGCGTCC 
TTTCTGCAGT TGTGCGGCCA GGCGCCGGCT GCCTCCATGT ACAACAGCGG CGGGGGCCAC 
ATCTCGCAGG ACACCATCCG CGCCTGGGAG ACGCACGAGT TGGATGTGAG TGGCGCCAGT 
GGTCACGATC GTGCGTCCTC TGTGGCCGCG GCGGGGGCGG CGCGAAACCA TCTCAGCGAG 
CTGCGCGTGG TCAAGGTGGT TGGTGGCGAG AATCGCGATG CTCAGTACGC CGCCCTGGCA 
CGCGGCGCGC ACTGCGTCGT CGGCACGGTG GGCCAGCTGC AAGTACTTCT CGAGGACCGC 
CTGCTCTCGC TCGGCAACAC GCAGTTCGTC GTGATGGACG AGGCAGATCG GATGATCGAT 
GAGCAGCAGG AGGAGCGGCT TACTGCAGTC CTAGAGCGGT GCCCGCAGCC GCGGCAGACA 
GTCATGTTCA CAGCGACGCT GAGCGTGGCC TGCGCGGCAG TGGCGAAGCG CTACCTCGCC 
AAGGCCGGCT ATTACCTGGT GCGCACACCG TACCGGTGCG CCTCGATCTG CCAGTCCTTC 
GAGCTGGTCG CCGACACCGG CGCCTCAGTC ACGGGGACTG CAGAGGGCCC CGTCCCCGCC 
GCGTCTGGCG CTGCCACAGC GACGGCAGAG GGGCGGCAAA AGAAGCCGTC TCGGCAACAA 
GGACACACCG AGAAGGGCGA GCCGCAACCG CCGCAGCGGC AGCGTCTGCT GCACCCTCTT 
ATACACGCCC AGAAGTTTGT GCGGTTGGTG GCGTGGCTCG TCTACGGCAC CGGCCCGATC 
ATCGTCTTCG CCAACGAAAA GTCGACGTGC GACGCGCTGT GGGAGGAGCT GCGTGCCGAG 
GCGGAGCACC TGGACGCACA GCAGGAATAC TACACAATCG AGGACATTGT CGGCCCGCCG 
CCGGACGGCT TGTCGTTTCG CGAAGACACC GCGGTTGGTC AAGGGGCAAC GTCGTCCAGC 
GTGGCGGCGC GGCGCACGCC GACGTTGGCG AACCTAACCT CCGTTGCCGT GGTGCACTCG 
GAGCTGTCGC AGACGGAGCG GCGCTCTCTC GTGGCGCAGT TCCAGCGGCG TCAGCGGCAC 
GTTCTCATCA CGACAGACCT TCTCGCTCGT GGCCTTGACG TGGCGGGGGT GACGCTTGTG 
ATCAACTACG ACGTGCCGTG GGCAGCCTCT GCCTCGCCGA GCGACGCTGT GACGCTCTAC 
ATCCACCGCA TCGGGCGTAC CGGCCGTGCA GGCTCCACCG GCGTCGCCGT AACTTTCGTG 
ACGTTGCCTG CCGCGATGGT ACAACGGGCC GAGGAGGTGG CGAGGGAGGA GGCGAGGGAC 
GCTGCGCCGA AGCCGCCTGA AGGCCGCACG AAACCGCCCA GCGCAGCACC CCGCGTGTAT 
GGCGACGAGG ATGACGACCT CGCCGCCCTC GGTGAGCTCT TCGACGGCGA CACGCGTAAT 
GTCTGTATGG CAGGGGCAGC GGCGCTATCC ACCACTGCCG CAGATAATGC CAGCGGCCGT 
CGTCGCCGCC GCGGTGACGA CGACAGCGAC GACACAGAAG TGGACAGCAG CAACGACAGC 
GATGACGACA ACGATGATGA GGACACCAAG AAGGAGGGCG CCACCGGAAA TACGAGCAAC 
ACCGCGAATC ACAACGGCAA TGGCATGCGG AAGCCGCCGA GGAAGCGCCG CAAGCGCTCG 
GTCGCCACCT TCGCCAGCGA CGTCGCGGTG CTGCGGCCCC TCTGGTCCTT CCTCGTCGAG 
TGTGCCGAGG GCGAGGGATG CCGCGATGGA GCGGCGGCGC TGCGGCGCGG CACATACGCA 
AAGATCAGTG TTCCGGCGGC GCTGGGGTGC ATCATGGCGC AGCTCTCTGC CACCTCCCAG 
TACGGCCGCA TTACCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_05_0590 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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