LINJ_02_0490 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_02_0490
Entrez Gene ID	5066184
Full Name	conserved hypothetical protein
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001462702.1	XP_001462739.1	conserved hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_02_0490 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_02_0490 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc00208	XM_001462702.1 Latest version!	Leishmania infantum JPCM5 conserved hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_02_0490 ( XM_001462702.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001462739.1 Leishmania infantum JPCM5 conserved hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OLc00208
Clone ID Related Accession (Same CDS sequence)	XM_001462702.1
Accession Version	XM_001462702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	conserved hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009387). COMPLETENESS: incomplete on both ends.

ATGCAGTCAC TGACCTCATC CGGCGTGATT GGCCGCTCGG TGTCGGCCAG GAAGACCCCT 
GCCCCCATTT CACTGCGAGA AGACGTGGGC AGCCTCCGTG CTGGTGGCGC GCGCACGAGT 
GCGGCAAGGG AAGGACCAGG CAGCACTGCG CGGTCTGCTT CAGCCAGAAC AGCTTCTCTG 
ACCACGTTGG GTAAGGCTGC GGCCAGAGCG GCGGTTGGTA ATGAGGCAAG TGGTTTCTGC 
CGGAGCTCAT CACGCGGCAG CGGCAGTGCG GGTCGATGCA GCCCGCAACG GTTTGGACAC 
GGCGTTGGTG AGCAGCAAGT CCGCCCTCGC TCGACGGTCA CGCTGGACTC ACCGAATCAG 
CACCTCTCTC CCGCCCCACA CGAGTCTTTG GCAGGAGTAT CGACCACCGC CGCTCCACCA 
ACGTCAAGCG ACGCCCGCAC AGGCGCGTTC CCGGCGACTC GTGAAGCGCC CAAGCGATCA 
AAGGTACAAA TTCGGAGCAT TCGCGTGCGC AGTGCGCCCG ATGAGCCATC AGGGCCGACG 
CGGCCCGTTG CCGGCAGGGA CACCGCACCA CCGCACCAGC AATGCCAACT TTCGAAGTCT 
GCCGAAGCGA CCACCGCGTC GGCGCCTGCG CGCATCTTTT CCGCCGCGGC GACGATTACG 
TCGAGCCCGC CAGCACACAC AAGCACGACC AGCTCCGCAG TGTCCATACC ACGCTCTCGC 
ACTGCTCGCA CCTCGGCCTC CTTGGAGGCA TTTTTGCTGA GCTTCCAGAA CTGCCTCCAC 
AGACAAGAGA TTTTGTTCCA GCGTGTCGAC ACCGCAGTCG CCCAACTCGA GAGTGCGTGC 
GTCAACGCCT GCGGTGCGAC GGTTTCCCCG CCTTCCTCGG CCCCGACAGC ACCGGCCCAG 
ACAGCCTCTC TTTGGGGCAG CGGCTCGCGG CCGCAGTACG TGTTGCCATC GCACATATCT 
TCCTGCCCCC CCGCGGCGGC CGCGGCCGGT GCGTCGCCGA CGCGACCTAA CAAGACTGTA 
ACCTTTCCAG GTGCTGTTAC CGGAGCAAGG CGCAGCGACG GCGCCAATGA CATTGCCAGC 
TCTAACATGC ACACGGACGG CGCGCCGGTG CTCTCTGTCT CTTCCTCCCA GCAGGAGCTG 
CCGGTCACCG ACGCTTCTCA CCACGCGCAG CGGCATCCGC CGAGCCAGAG CACGGGGTCT 
TCGACTCTTT CACTGTCCGT TGTCGTGATG CAGCCCGCGT TGGAGCGCGT GGGCGGCAAC 
GACGGCGGGT CGCCGATGGT GTGTGCCTTC GCTCAAGGCC TCCTATCCCC GCCCCAGCAG 
ACGTCGCCGC TCCTCACGTT GCCGACCTAC GTTCGGCTTG ACGGCGCGGC ACTGACACCG 
ACCTCGGCGG CGCCAACTAG GCTCTCTCAT CCACACCCCC TGTTTCCTTG CTCGATGCCG 
GGGACACCGC TGTCGCCGGC TCGGTTGGCC ACCACGCAGC AGCAACTCCC CACGAGCAAC 
ACCGGGTGCA CAGGCAGCCT GCCACTGAAC ACAAAGGTGA CGTCTCTGGT GACCGAATCC 
CCGCCCATCT CCGGCAGCGG AGGGGCACTG GGCAGGCAGC AATACCCAGC GGCGGACGCG 
GAGATCATCG CAGGTGACCC AGTCACGGAA GCCCCTATGC TGCCCGCGCC TGCCGAAGGA 
ACGGATGCAC ACGAGGCAGC CGCGCCGTGT GGCGTGGCGA GCACCGCCGC GGAGAACACC 
GCACCTGAGG ATGCGGAGGA GAGCGCCGGG GGTAGACGAA AAGGCGCTGC TGCGACCGAG 
TCGTCCAGTG ACGAGAGCGC CTCCGCTTCT CCGATTGTGC ACAGTGCGCA GATGCCCTAC 
CTCACCATCA CCATTTCTGG CGCAGACCAG AGCTTTAACA GCGGCTGCGG CGGCGCAGGG 
AGCGATGGCG TGCCGACGTG GTCCAGCGCC CCGCCTGGGC ACGCGAAGAT CGGAAACCTT 
CGCATCGGCG GCATTCCCGA CTCCTCCGTG ATAAGCGTCT TCAGCGAGAG CGCGGACAGC 
GTGTCGCTTT CGCCCCTCTC GCGCCCGCCG TCGATTTGGG TAGGTAGCCC GAGGACGCGA 
AGCAATCTCG GTCCGACGTC GCCGCATCAA GCGCAGCTGT CGCTGCAACA GCACTCCTCG 
CTTGGCGGTA GCAGCATCAG CACAGCATGG ATTTCACCCC GCCGTGTGCC GAACAGCCAC 
AACAGCCGCA GCAGCAGCAA CGCCGCTGGG GCGACGCAGG CATCGGCGAA CCGGTCCCCC 
CGCACAGTGG CCGCGTTGGA GAACCCGCGA CACGCGTCGA CGGCGGTGAA CTCCGCCAGC 
TCTCTACCGT CGATGAGTCA ACAGCAACCG CAGCAGCGCA GCTCAGGGGA CCTGCTCTCG 
CCCTTGTCCG GGCAGTGGCT GTTGTCGCCC TCCCCGACAC CAACAGTTTC GCGGCGTGCG 
AGTGGCGTGG CGCACCGCAG TAGCGTCCAG ATGACCTCTG TCAACTTGTC AGGCAGCGGC 
ACCCAGAACA TCGGCGGCAA CAACAGCAGC GGCAACGCTG GTGGGCTGGC AACGGTGATG 
TCATCCGCTA CAGTGGAGGG GAACGTCGTC TCCAGCCTTT CCTCAACGTC TCCTCTGGTG 
CCGCCGTGCT TTCCTGACAG CGGCGGCGTT GTCACGGGTG CGACTCAAGC AGTGACGTCG 
CCGATAACCG CCTGTCGCCA ACTCACCCTA TCGTCATCGC CGTCGGCTGA GGAAGCCGAT 
CGCGATGACT CCTTTCAGGA AACCTCGCTG CGGTCGCCCA TTGGCCTGGC CGAGGAGGCG 
GACGACGAAG ACGCGCAGCA GCTGCAACAG CTGGCAAACC GCTACGCCCC GCCACCGCCA 
CCACCGTCGT TGACAGAGTT GCAGCAGCAG CAGAACCAGC GCCGTCGCTG GAGTGCGCCA 
CCAGTGCCCA CCATGAGACG TCCTCCCATG GCACCTCTGC GTCATCCCGA CGCTGCCTGC 
CCGGCAGCGC TTCGGAATGA GGAGGAGCGG CTGCGCAGCT CCACGCCACC GGTTGCGAGG 
CCAGAGCAGT GGCTGTCGTT TGAGCAGCAG CACGAGCGGC TGCACCTGCC CGAGTGCTGG 
CGTGATAGCG AGGTGATCGT CGTCGACCAT GTGGAGAAGG ACAGGGAACG TGATTCCGAG 
GAGGCAGAGC GGCTGCTAGA GCTGGAGGAG GAGCTGCTGA GAGAGCTGCG GCAAAACCAG 
TCAGACCGGA TGGACGGCGA CGGTCTACTT CGGCGGGGCC GGCGACGGGG AAGTCGGCAC 
AGGCACTTGA TGGCAGACGC CAACGCCGCC GGCGAGACGA TAGACGAGGA GGAGCTTGAT 
TGGGGCGATG ACTCAGCCGA TGGGGCCGCG GCGGACGCTG CCTACGAAGC ACGCCTGGCG 
CGCTACGGCA GAGCGATGTT GCTGCACCGA CGCGGCCGCT CTAGCGCCTC GCTGCACCGC 
GACGGCGGCG AGGGGTGCTC GTGCGGCAGC TGCCCATCTG CCACGAGCTG GTGTTGCCGG 
CGCCGACCCG TTAGCGGAGG AGACCGGCAG GGCTTCCCCT CTGACGCCAC CGCCTCGTTG 
AGAGGGGCGC CCAGCGGTGA GAGCACCACC CTCCCAGCTG CCACGGGCGC TCCTTCAGGC 
TCGCCCGTGC TACCCATGCG CAACTTCAGC CGTCGCCACT CAGCCCCGGC GACCATCTGG 
GGCGCCGACC ACGGGACAGG GTGGCGTGAC CTGCAGGCAC GTTTTGAAGA GCTCATCAGT 
ATTCAGCAGC GCAGTCGCGG AAGTGAGCGG GATGGCGCCG ATGGCACAAC TCTTGACGAG 
CAGTCGTTAT CCACACTGGC GAACAACACG GCCTCCAGTC CCATGAAAGA AGGTCTCGCC 
AGCGGTATAT CACGTCGCCG TCGCAGCCGC AGCTTTAGCC ACGAAGTCAC CGCCTGCCCG 
CCAGATGCGG TGGACAGGCG GGCACAGAGC GCCTCTCCTC CGACGGGCGA CCTCAGCAGT 
TCCTACCGCG ACAACATGCC GCATGACGGC GACGGGAACA GTGGTTTGCC TGCTCCATCT 
CCGCACGCTG CTGCTGCGGC ACCGACGCCG CTCGAGCGGA CACACACCCA CCCGGGCTCA 
AGCGGCTACC GCCGCGTCCA GTGGGGGAGG GAAGCTGAAG CCGATGCGGC GCCGTCGCCG 
CCCTCGCAGG CACCCGAAAA GCAGCGCATC TCTTCCGCCG CTCTCTCTCC ATACTCATCC 
ACTCCTCCGT CGCCGTCAGA GCAGGACGGC ACGCTAGGGC AGTCGAGCCA CGCCCGCGTG 
TCGGCTGTCT GCAAGAATCC GCCCGCGTTT CTCACGTCTC CGAGGCACCC GTCCCTGACG 
GTGAACGCGG CCCACACAGC GAGCACGAGG GACTCTGCCG AGACACACAA GGAGGCCGCC 
GCGTACGACA GTGACGTGGA AGAAGACGAA AGGGATCCGA AGAGCGCCGT GGCCGCGCGC 
ACGCCTGTGG CGTCAACCGC GCGGCGGACA CCGAGGCTGG AATTCATGCG CCCTTCCCGC 
ACGGCGTCAT CGATGTGCAT ACTGGGCCGG CCGCGACGCA CCATGGGGCT GCACAGCGCG 
CCGGGAAAGG GGCGCCAAAA CACGAAAGCC GCCCAGGCGG GTCGCGCCGA CGGCGAGTCC 
AAGACGGGTG CCACGCCGTC AACAACCCGC AGGCTATCCG CGAGGCAGAA GCCGCAGGCG 
GCAGCGGCGT CTGCTGTGAC GGCCGCCAGC GGAAGGCGTG GCAACACTGC GGCTTTGAGG 
TCTGGCAGCT GCGGCTCTGC CAGAGGCTCG AGCAGTGTCG GCAGCGGCGG AGGGCGCGCT 
ACTCGCTGCA GCGGCGCGAA CCGTGCTTCT CTCGACACCA CGGCCACCGC TTCCATCGTC 
CCTACGAAGG AGACAGATGA CGAGGCACCG ACGGCCGCGA CGGAGTCGAA CAGCCACGGC 
ACCACTCGCG CCGACACGGG GACAACGATG TCAGCGTCGC ACCCATCTGA CCACGCGCTC 
GATGGCGGCG AGCTTGTAGA GCCGCTCTCG GCGGTGTTTG TGGTCGCTGC CGCCGCCGCG 
CCAGCCGCCG CGCCGTCGCC GCCAACGACC ACGCAGAAAA GCGCGCGGGA CAGCGGCACG 
CAGCGCGGAC GACTGCGAGA CAGCAAGGCC AGCTCCAGCC GGGACGCTGA CAAGGACCAC 
GACGCTTCCC TCTCGTCGCC TCCACAGCAT CGGCACCGCA GACACGCTGG GGCAGAGGGT 
GAGACAGCCG TTTCGGCCCG CCCGCCGCAC AAGGCAAGTG CACGGCATGC ACCGGGGCTT 
CCAGAAAACG AAGCGAGGCA TCCGGACGGC GGTGCCGCAG CAGGAACGAC CGCGTTGGAG 
GGCGCTGACT CGCGCGCGCT GCTGCGACGA CTTGCCCAGC AGAAAGACCG CACCCGGGCC 
ATTGAAGAGC GCCTGCGATT CGGCGTCAAC AGCACCGCCT TGGCGAACCC TAATAGCGGC 
AGCCACAGTG CCCGCAGCGG CTGCGGCAGC GGTTTCGTGT GGAGTGACGG GCACATCACA 
GGCAGATGGG GACTGGAGTT GGAGAGCTGC ACGCCGACGC GCGGCGCCGC GCGGGCGTGC 
ATGGCTGCCG ACCTTGCTCG CCAGGACTTC AGCACACCGT CGAACATGTA CTCGGTGAGT 
GGAGCCACCG GAGACGCCAC CTTGGACACT CCTGCGACTC GCGTCAGCAC AGCTCGGCAC 
GGCGGCGGTG CCGCGGCAGA CGCCCCCCAC GTGACGGTGG TCGACCCTGC TGAAGCCACG 
GCTGTTTACG GTGTGCACGC CGTTCATTCC GGCGCAGGTG CCGGCGCGGC CGCAGCCGCA 
CAGCAGACCT CATCCAATCC ACGCCGGCGC CGCGTCGATC TGCGCTCGCG CCTGGTGTCG 
AAGCTAACTG CCGTCTCGTC GTCTCTGTCA GCGAGCCCCG CGAAGCGGCA CAAGGGCTGC 
ACTGCAACCA TCACAACGGC CACCAACGCC ACCGCTGCTG CAACGGCGGC CTCTACCCAA 
AGTTTCCGCC GCGCCGAGAG CGCCTACAGT ATACGCTCTC GCTCCCCCAC GTACGTCACC 
ATAATGCACC ATGCGGGCTA CGGCGATCGC CGCCGGCACA GCATCAGCGG CGACGTGCTG 
CCACCCACTA CGTTATCCAT GCCCGAGCGC CAGCTGCGTT CTTCGCGGGC GCCGGTGGCC 
AGTCAGCTGA TCGACTGGGC TCGGGGAGGC GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001462739.1
CDS	1..6276
Translation	MQSLTSSGVIGRSVSARKTPAPISLREDVGSLRAGGARTSAAREGPGSTARSASARTASLTTLGKAAARAAVGNEASGFCRSSSRGSGSAGRCSPQRFGHGVGEQQVRPRSTVTLDSPNQHLSPAPHESLAGVSTTAAPPTSSDARTGAFPATREAPKRSKVQIRSIRVRSAPDEPSGPTRPVAGRDTAPPHQQCQLSKSAEATTASAPARIFSAAATITSSPPAHTSTTSSAVSIPRSRTARTSASLEAFLLSFQNCLHRQEILFQRVDTAVAQLESACVNACGATVSPPSSAPTAPAQTASLWGSGSRPQYVLPSHISSCPPAAAAAGASPTRPNKTVTFPGAVTGARRSDGANDIASSNMHTDGAPVLSVSSSQQELPVTDASHHAQRHPPSQSTGSSTLSLSVVVMQPALERVGGNDGGSPMVCAFAQGLLSPPQQTSPLLTLPTYVRLDGAALTPTSAAPTRLSHPHPLFPCSMPGTPLSPARLATTQQQLPTSNTGCTGSLPLNTKVTSLVTESPPISGSGGALGRQQYPAADAEIIAGDPVTEAPMLPAPAEGTDAHEAAAPCGVASTAAENTAPEDAEESAGGRRKGAAATESSSDESASASPIVHSAQMPYLTITISGADQSFNSGCGGAGSDGVPTWSSAPPGHAKIGNLRIGGIPDSSVISVFSESADSVSLSPLSRPPSIWVGSPRTRSNLGPTSPHQAQLSLQQHSSLGGSSISTAWISPRRVPNSHNSRSSSNAAGATQASANRSPRTVAALENPRHASTAVNSASSLPSMSQQQPQQRSSGDLLSPLSGQWLLSPSPTPTVSRRASGVAHRSSVQMTSVNLSGSGTQNIGGNNSSGNAGGLATVMSSATVEGNVVSSLSSTSPLVPPCFPDSGGVVTGATQAVTSPITACRQLTLSSSPSAEEADRDDSFQETSLRSPIGLAEEADDEDAQQLQQLANRYAPPPPPPSLTELQQQQNQRRRWSAPPVPTMRRPPMAPLRHPDAACPAALRNEEERLRSSTPPVARPEQWLSFEQQHERLHLPECWRDSEVIVVDHVEKDRERDSEEAERLLELEEELLRELRQNQSDRMDGDGLLRRGRRRGSRHRHLMADANAAGETIDEEELDWGDDSADGAAADAAYEARLARYGRAMLLHRRGRSSASLHRDGGEGCSCGSCPSATSWCCRRRPVSGGDRQGFPSDATASLRGAPSGESTTLPAATGAPSGSPVLPMRNFSRRHSAPATIWGADHGTGWRDLQARFEELISIQQRSRGSERDGADGTTLDEQSLSTLANNTASSPMKEGLASGISRRRRSRSFSHEVTACPPDAVDRRAQSASPPTGDLSSSYRDNMPHDGDGNSGLPAPSPHAAAAAPTPLERTHTHPGSSGYRRVQWGREAEADAAPSPPSQAPEKQRISSAALSPYSSTPPSPSEQDGTLGQSSHARVSAVCKNPPAFLTSPRHPSLTVNAAHTASTRDSAETHKEAAAYDSDVEEDERDPKSAVAARTPVASTARRTPRLEFMRPSRTASSMCILGRPRRTMGLHSAPGKGRQNTKAAQAGRADGESKTGATPSTTRRLSARQKPQAAAASAVTAASGRRGNTAALRSGSCGSARGSSSVGSGGGRATRCSGANRASLDTTATASIVPTKETDDEAPTAATESNSHGTTRADTGTTMSASHPSDHALDGGELVEPLSAVFVVAAAAAPAAAPSPPTTTQKSARDSGTQRGRLRDSKASSSRDADKDHDASLSSPPQHRHRRHAGAEGETAVSARPPHKASARHAPGLPENEARHPDGGAAAGTTALEGADSRALLRRLAQQKDRTRAIEERLRFGVNSTALANPNSGSHSARSGCGSGFVWSDGHITGRWGLELESCTPTRGAARACMAADLARQDFSTPSNMYSVSGATGDATLDTPATRVSTARHGGGAAADAPHVTVVDPAEATAVYGVHAVHSGAGAGAAAAAQQTSSNPRRRRVDLRSRLVSKLTAVSSSLSASPAKRHKGCTATITTATNATAAATAASTQSFRRAESAYSIRSRSPTYVTIMHHAGYGDRRRHSISGDVLPPTTLSMPERQLRSSRAPVASQLIDWARGGE

Target ORF information:

RefSeq Version	XM_001462702.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 conserved hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001462702.1

ORF Insert Sequence:

ATGCAGTCAC TGACCTCATC CGGCGTGATT GGCCGCTCGG TGTCGGCCAG GAAGACCCCT 
GCCCCCATTT CACTGCGAGA AGACGTGGGC AGCCTCCGTG CTGGTGGCGC GCGCACGAGT 
GCGGCAAGGG AAGGACCAGG CAGCACTGCG CGGTCTGCTT CAGCCAGAAC AGCTTCTCTG 
ACCACGTTGG GTAAGGCTGC GGCCAGAGCG GCGGTTGGTA ATGAGGCAAG TGGTTTCTGC 
CGGAGCTCAT CACGCGGCAG CGGCAGTGCG GGTCGATGCA GCCCGCAACG GTTTGGACAC 
GGCGTTGGTG AGCAGCAAGT CCGCCCTCGC TCGACGGTCA CGCTGGACTC ACCGAATCAG 
CACCTCTCTC CCGCCCCACA CGAGTCTTTG GCAGGAGTAT CGACCACCGC CGCTCCACCA 
ACGTCAAGCG ACGCCCGCAC AGGCGCGTTC CCGGCGACTC GTGAAGCGCC CAAGCGATCA 
AAGGTACAAA TTCGGAGCAT TCGCGTGCGC AGTGCGCCCG ATGAGCCATC AGGGCCGACG 
CGGCCCGTTG CCGGCAGGGA CACCGCACCA CCGCACCAGC AATGCCAACT TTCGAAGTCT 
GCCGAAGCGA CCACCGCGTC GGCGCCTGCG CGCATCTTTT CCGCCGCGGC GACGATTACG 
TCGAGCCCGC CAGCACACAC AAGCACGACC AGCTCCGCAG TGTCCATACC ACGCTCTCGC 
ACTGCTCGCA CCTCGGCCTC CTTGGAGGCA TTTTTGCTGA GCTTCCAGAA CTGCCTCCAC 
AGACAAGAGA TTTTGTTCCA GCGTGTCGAC ACCGCAGTCG CCCAACTCGA GAGTGCGTGC 
GTCAACGCCT GCGGTGCGAC GGTTTCCCCG CCTTCCTCGG CCCCGACAGC ACCGGCCCAG 
ACAGCCTCTC TTTGGGGCAG CGGCTCGCGG CCGCAGTACG TGTTGCCATC GCACATATCT 
TCCTGCCCCC CCGCGGCGGC CGCGGCCGGT GCGTCGCCGA CGCGACCTAA CAAGACTGTA 
ACCTTTCCAG GTGCTGTTAC CGGAGCAAGG CGCAGCGACG GCGCCAATGA CATTGCCAGC 
TCTAACATGC ACACGGACGG CGCGCCGGTG CTCTCTGTCT CTTCCTCCCA GCAGGAGCTG 
CCGGTCACCG ACGCTTCTCA CCACGCGCAG CGGCATCCGC CGAGCCAGAG CACGGGGTCT 
TCGACTCTTT CACTGTCCGT TGTCGTGATG CAGCCCGCGT TGGAGCGCGT GGGCGGCAAC 
GACGGCGGGT CGCCGATGGT GTGTGCCTTC GCTCAAGGCC TCCTATCCCC GCCCCAGCAG 
ACGTCGCCGC TCCTCACGTT GCCGACCTAC GTTCGGCTTG ACGGCGCGGC ACTGACACCG 
ACCTCGGCGG CGCCAACTAG GCTCTCTCAT CCACACCCCC TGTTTCCTTG CTCGATGCCG 
GGGACACCGC TGTCGCCGGC TCGGTTGGCC ACCACGCAGC AGCAACTCCC CACGAGCAAC 
ACCGGGTGCA CAGGCAGCCT GCCACTGAAC ACAAAGGTGA CGTCTCTGGT GACCGAATCC 
CCGCCCATCT CCGGCAGCGG AGGGGCACTG GGCAGGCAGC AATACCCAGC GGCGGACGCG 
GAGATCATCG CAGGTGACCC AGTCACGGAA GCCCCTATGC TGCCCGCGCC TGCCGAAGGA 
ACGGATGCAC ACGAGGCAGC CGCGCCGTGT GGCGTGGCGA GCACCGCCGC GGAGAACACC 
GCACCTGAGG ATGCGGAGGA GAGCGCCGGG GGTAGACGAA AAGGCGCTGC TGCGACCGAG 
TCGTCCAGTG ACGAGAGCGC CTCCGCTTCT CCGATTGTGC ACAGTGCGCA GATGCCCTAC 
CTCACCATCA CCATTTCTGG CGCAGACCAG AGCTTTAACA GCGGCTGCGG CGGCGCAGGG 
AGCGATGGCG TGCCGACGTG GTCCAGCGCC CCGCCTGGGC ACGCGAAGAT CGGAAACCTT 
CGCATCGGCG GCATTCCCGA CTCCTCCGTG ATAAGCGTCT TCAGCGAGAG CGCGGACAGC 
GTGTCGCTTT CGCCCCTCTC GCGCCCGCCG TCGATTTGGG TAGGTAGCCC GAGGACGCGA 
AGCAATCTCG GTCCGACGTC GCCGCATCAA GCGCAGCTGT CGCTGCAACA GCACTCCTCG 
CTTGGCGGTA GCAGCATCAG CACAGCATGG ATTTCACCCC GCCGTGTGCC GAACAGCCAC 
AACAGCCGCA GCAGCAGCAA CGCCGCTGGG GCGACGCAGG CATCGGCGAA CCGGTCCCCC 
CGCACAGTGG CCGCGTTGGA GAACCCGCGA CACGCGTCGA CGGCGGTGAA CTCCGCCAGC 
TCTCTACCGT CGATGAGTCA ACAGCAACCG CAGCAGCGCA GCTCAGGGGA CCTGCTCTCG 
CCCTTGTCCG GGCAGTGGCT GTTGTCGCCC TCCCCGACAC CAACAGTTTC GCGGCGTGCG 
AGTGGCGTGG CGCACCGCAG TAGCGTCCAG ATGACCTCTG TCAACTTGTC AGGCAGCGGC 
ACCCAGAACA TCGGCGGCAA CAACAGCAGC GGCAACGCTG GTGGGCTGGC AACGGTGATG 
TCATCCGCTA CAGTGGAGGG GAACGTCGTC TCCAGCCTTT CCTCAACGTC TCCTCTGGTG 
CCGCCGTGCT TTCCTGACAG CGGCGGCGTT GTCACGGGTG CGACTCAAGC AGTGACGTCG 
CCGATAACCG CCTGTCGCCA ACTCACCCTA TCGTCATCGC CGTCGGCTGA GGAAGCCGAT 
CGCGATGACT CCTTTCAGGA AACCTCGCTG CGGTCGCCCA TTGGCCTGGC CGAGGAGGCG 
GACGACGAAG ACGCGCAGCA GCTGCAACAG CTGGCAAACC GCTACGCCCC GCCACCGCCA 
CCACCGTCGT TGACAGAGTT GCAGCAGCAG CAGAACCAGC GCCGTCGCTG GAGTGCGCCA 
CCAGTGCCCA CCATGAGACG TCCTCCCATG GCACCTCTGC GTCATCCCGA CGCTGCCTGC 
CCGGCAGCGC TTCGGAATGA GGAGGAGCGG CTGCGCAGCT CCACGCCACC GGTTGCGAGG 
CCAGAGCAGT GGCTGTCGTT TGAGCAGCAG CACGAGCGGC TGCACCTGCC CGAGTGCTGG 
CGTGATAGCG AGGTGATCGT CGTCGACCAT GTGGAGAAGG ACAGGGAACG TGATTCCGAG 
GAGGCAGAGC GGCTGCTAGA GCTGGAGGAG GAGCTGCTGA GAGAGCTGCG GCAAAACCAG 
TCAGACCGGA TGGACGGCGA CGGTCTACTT CGGCGGGGCC GGCGACGGGG AAGTCGGCAC 
AGGCACTTGA TGGCAGACGC CAACGCCGCC GGCGAGACGA TAGACGAGGA GGAGCTTGAT 
TGGGGCGATG ACTCAGCCGA TGGGGCCGCG GCGGACGCTG CCTACGAAGC ACGCCTGGCG 
CGCTACGGCA GAGCGATGTT GCTGCACCGA CGCGGCCGCT CTAGCGCCTC GCTGCACCGC 
GACGGCGGCG AGGGGTGCTC GTGCGGCAGC TGCCCATCTG CCACGAGCTG GTGTTGCCGG 
CGCCGACCCG TTAGCGGAGG AGACCGGCAG GGCTTCCCCT CTGACGCCAC CGCCTCGTTG 
AGAGGGGCGC CCAGCGGTGA GAGCACCACC CTCCCAGCTG CCACGGGCGC TCCTTCAGGC 
TCGCCCGTGC TACCCATGCG CAACTTCAGC CGTCGCCACT CAGCCCCGGC GACCATCTGG 
GGCGCCGACC ACGGGACAGG GTGGCGTGAC CTGCAGGCAC GTTTTGAAGA GCTCATCAGT 
ATTCAGCAGC GCAGTCGCGG AAGTGAGCGG GATGGCGCCG ATGGCACAAC TCTTGACGAG 
CAGTCGTTAT CCACACTGGC GAACAACACG GCCTCCAGTC CCATGAAAGA AGGTCTCGCC 
AGCGGTATAT CACGTCGCCG TCGCAGCCGC AGCTTTAGCC ACGAAGTCAC CGCCTGCCCG 
CCAGATGCGG TGGACAGGCG GGCACAGAGC GCCTCTCCTC CGACGGGCGA CCTCAGCAGT 
TCCTACCGCG ACAACATGCC GCATGACGGC GACGGGAACA GTGGTTTGCC TGCTCCATCT 
CCGCACGCTG CTGCTGCGGC ACCGACGCCG CTCGAGCGGA CACACACCCA CCCGGGCTCA 
AGCGGCTACC GCCGCGTCCA GTGGGGGAGG GAAGCTGAAG CCGATGCGGC GCCGTCGCCG 
CCCTCGCAGG CACCCGAAAA GCAGCGCATC TCTTCCGCCG CTCTCTCTCC ATACTCATCC 
ACTCCTCCGT CGCCGTCAGA GCAGGACGGC ACGCTAGGGC AGTCGAGCCA CGCCCGCGTG 
TCGGCTGTCT GCAAGAATCC GCCCGCGTTT CTCACGTCTC CGAGGCACCC GTCCCTGACG 
GTGAACGCGG CCCACACAGC GAGCACGAGG GACTCTGCCG AGACACACAA GGAGGCCGCC 
GCGTACGACA GTGACGTGGA AGAAGACGAA AGGGATCCGA AGAGCGCCGT GGCCGCGCGC 
ACGCCTGTGG CGTCAACCGC GCGGCGGACA CCGAGGCTGG AATTCATGCG CCCTTCCCGC 
ACGGCGTCAT CGATGTGCAT ACTGGGCCGG CCGCGACGCA CCATGGGGCT GCACAGCGCG 
CCGGGAAAGG GGCGCCAAAA CACGAAAGCC GCCCAGGCGG GTCGCGCCGA CGGCGAGTCC 
AAGACGGGTG CCACGCCGTC AACAACCCGC AGGCTATCCG CGAGGCAGAA GCCGCAGGCG 
GCAGCGGCGT CTGCTGTGAC GGCCGCCAGC GGAAGGCGTG GCAACACTGC GGCTTTGAGG 
TCTGGCAGCT GCGGCTCTGC CAGAGGCTCG AGCAGTGTCG GCAGCGGCGG AGGGCGCGCT 
ACTCGCTGCA GCGGCGCGAA CCGTGCTTCT CTCGACACCA CGGCCACCGC TTCCATCGTC 
CCTACGAAGG AGACAGATGA CGAGGCACCG ACGGCCGCGA CGGAGTCGAA CAGCCACGGC 
ACCACTCGCG CCGACACGGG GACAACGATG TCAGCGTCGC ACCCATCTGA CCACGCGCTC 
GATGGCGGCG AGCTTGTAGA GCCGCTCTCG GCGGTGTTTG TGGTCGCTGC CGCCGCCGCG 
CCAGCCGCCG CGCCGTCGCC GCCAACGACC ACGCAGAAAA GCGCGCGGGA CAGCGGCACG 
CAGCGCGGAC GACTGCGAGA CAGCAAGGCC AGCTCCAGCC GGGACGCTGA CAAGGACCAC 
GACGCTTCCC TCTCGTCGCC TCCACAGCAT CGGCACCGCA GACACGCTGG GGCAGAGGGT 
GAGACAGCCG TTTCGGCCCG CCCGCCGCAC AAGGCAAGTG CACGGCATGC ACCGGGGCTT 
CCAGAAAACG AAGCGAGGCA TCCGGACGGC GGTGCCGCAG CAGGAACGAC CGCGTTGGAG 
GGCGCTGACT CGCGCGCGCT GCTGCGACGA CTTGCCCAGC AGAAAGACCG CACCCGGGCC 
ATTGAAGAGC GCCTGCGATT CGGCGTCAAC AGCACCGCCT TGGCGAACCC TAATAGCGGC 
AGCCACAGTG CCCGCAGCGG CTGCGGCAGC GGTTTCGTGT GGAGTGACGG GCACATCACA 
GGCAGATGGG GACTGGAGTT GGAGAGCTGC ACGCCGACGC GCGGCGCCGC GCGGGCGTGC 
ATGGCTGCCG ACCTTGCTCG CCAGGACTTC AGCACACCGT CGAACATGTA CTCGGTGAGT 
GGAGCCACCG GAGACGCCAC CTTGGACACT CCTGCGACTC GCGTCAGCAC AGCTCGGCAC 
GGCGGCGGTG CCGCGGCAGA CGCCCCCCAC GTGACGGTGG TCGACCCTGC TGAAGCCACG 
GCTGTTTACG GTGTGCACGC CGTTCATTCC GGCGCAGGTG CCGGCGCGGC CGCAGCCGCA 
CAGCAGACCT CATCCAATCC ACGCCGGCGC CGCGTCGATC TGCGCTCGCG CCTGGTGTCG 
AAGCTAACTG CCGTCTCGTC GTCTCTGTCA GCGAGCCCCG CGAAGCGGCA CAAGGGCTGC 
ACTGCAACCA TCACAACGGC CACCAACGCC ACCGCTGCTG CAACGGCGGC CTCTACCCAA 
AGTTTCCGCC GCGCCGAGAG CGCCTACAGT ATACGCTCTC GCTCCCCCAC GTACGTCACC 
ATAATGCACC ATGCGGGCTA CGGCGATCGC CGCCGGCACA GCATCAGCGG CGACGTGCTG 
CCACCCACTA CGTTATCCAT GCCCGAGCGC CAGCTGCGTT CTTCGCGGGC GCCGGTGGCC 
AGTCAGCTGA TCGACTGGGC TCGGGGAGGC GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_02_0490 cDNA ORF clone, Leishmania infantum JPCM5

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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