PF3D7_0700100 cDNA ORF clone, Plasmodium falciparum 3D7

Gene
Clones
gRNAs

Summary

Gene Symbol	PF3D7_0700100
Entrez Gene ID	9221962
Full Name	erythrocyte membrane protein 1, PfEMP1
Gene Type	protein-coding
Organism	Plasmodium falciparum 3D7

mRNA and Protein(s)

mRNA	Protein	Name
XM_002808860.1	XP_002808906.1	erythrocyte membrane protein 1, PfEMP1

Pathways from BioSystems
Gene Ontology

The following PF3D7_0700100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PF3D7_0700100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr05264	XM_002808860.1 Latest version!	Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_0700100), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PF3D7_0700100 ( XM_002808860.1 ) cDNA ORF clone, Plasmodium falciparum 3D7 -> XP_002808906.1 Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_0700100), partial mRNA.

Price & Availability↑

CloneID	OPr05264
Clone ID Related Accession (Same CDS sequence)	XM_002808860.1
Accession Version	XM_002808860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522080000000
Organism	Plasmodium falciparum 3D7
Product	erythrocyte membrane protein 1, PfEMP1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_004328). COMPLETENESS: incomplete on both ends.

ATGGTGAACC AAAATGGTGG TGGGGGTGGT AAGGATGAAG ATGCCAAATA TATGTTTGAT 
AGGATAGGGC AACAAGTGCA CGACGAAGTG AAGAATGGTG AGGCTAAGAA TTATGTTAGT 
GAATTGGAAG GAAAGTTGTC ATTTGCATCA ATTTTGGGGG AAACAGGTGG CTTTTATAAA 
ACGTGCGACA TTATAAAAAA TGAAGGTGAG AAACTTCTTC GTGCTCGCGA TGATCCGTGC 
GGAAATGGAA GTGCAAGTGA TAAACGTTTT TCTAAAGAAA GTGGCGGTGA ATGTGATGAT 
AAGAAAATAG AAGGTAATAA AAACAATAGT GAAGGTGGAG CCTGTGCTCC ATATAGACGG 
TTATCTCTAT GTAACAAAAA TATGGAAAAA AAGGGAACAA CGAAAATCAA GGATAAAAAT 
GATTTGCTGG CAGAAGTGTG TATGGCAGCA AATTACGAGG CACAGTCATT AATACCTTAT 
CATGACAAAT ATAAATTAAC TTATGGTGAT TCTAAAATAT GTACTGCCCT TGCACGAAGT 
TTTGCGGATA TAGGAGATAT TGTGAGAGGA AAAGATCTGT ATCGTGGTGG TGGTAGGGGA 
AGAAAACAAT TAGAAGAAAA TTTACAAAAA ATTTTTGGGA ATATATATGA CAAATTATTA 
GAGGAGAACC GGAGGAATGG GAAGAACAGG GAGATAGAAA AACGCTACGA AGGTGATAAA 
GAAAATTATT TTTTTAAATT ACGAGAAGAT TGGTGGACTG CGAATCGCGC CACAGTGTGG 
GAAGCTATCA CGTGCAGCGA CAGGCTAGGG GGTAATGCAT ATTTTCATGC AACGTGCGGT 
GAGAGTAGAA GACCATCTCA AGCTCAAAAC CAATGCCGCT GTAGCGACAA GCCAAAGGCC 
ATCAAGGCAG GTGGCAACGT AAGTATTGTC CCCACATATT TTGACTACGT GCCGCAGTAT 
CTTCGGTGGT TCGAGGAATG GGCAGAAGAT TTTTGTAGGA AAAAAAAAAA AAAAGTACAA 
AATTTGGAAC AACAATGTCG TGGACAAGAT ATAAGTGGTA ACCAACGATA TTGTAGCCGT 
AATGGCTACG ATTGCGAAAA AACTAAACCA GCGATTGGTA GATTGCGTAT GGGTAAAGGT 
TGCACTGACT GTTTTTTTGC ATGTAATCCT TACATTGATT GGATAAATAA CCAACGAAAA 
CAATTTGACA AACAAAAAAA CAAATACGAA ACAGAAATAT CAGGTGGTGG TGCTAGTGGT 
AGTAGTAGGC AAAGACGAGT TGCACGTGGT GGTAGTAATG TTGATGGATA TGAAAGTAAA 
TTTTATAAAA TACTTAAAGA CGACTATGAA ACCGTTGATG GTTTTTTGGA ATTATTAAAT 
AAAGAAAAAA CATGCACAAA AAAATTGAAG GATGACGACG AAGACGAAGG AACAATTAAT 
TTTAAAACCG TTAATAGTAG AGGTGGTGAT GGTAGTAATA AAACGTTTTA TCGTTCAAAA 
TATTGCCAAC CCTGTCCTCT ATGTGGAGTG AAAAAGAATG ATAATGGTAT TGGATGGGAA 
GAGAAAAATG ATAGTGAAGA ATGCAAGAGT ATAAACCTTT ATAAGCCTAT AAACCCAGAT 
AACGGTACTA AGATTGAAAT CCTTAAAAGT GGTGAAGGAA AAGAAGAAAT AAAAAAAAAA 
TTAGAGGCGT TTTGCGATCA AATAAATCGT GTTACAACAA ATGCTGCTAG TGGTGGCGCT 
GGTGGTAGTG GTAGTAAAAG TGATAGTAAG GAACTGTATG AAGAATGGAC ATGTTATCAC 
GTTAAACAGC TGGAGAAAGA AAATAAAGAA AATGGTGTGA ATGACGACGA CTATAACAAC 
GATGTAACAA CTGGAGGCGG ATTATGTATA TTGGAAACGA CGAACGGCTT AAATGTGCAA 
AAACAAAAGA CATTCCATAA TTTTTTTTAC TATTGGGTAG CACATATGTT AAAAGATTCC 
ATATATTGGA GAACAGAAAA ACTTGAGAGA TGTTTAAAAA ATGGTAATCC TATGAAATGT 
AGAAATGGAT GCCATGGTAA ATGTGATTGT TTTAAAAGAT GGATTGATAA AAAAGAAAAA 
GACGAATGGG ACAAAATAAA AGAACATTTT GGCAACCAAG AAGATTTTAA GAATAAAGGA 
GAAAGTGGCG AAGGTGACAT GTTTGGCAGA GCATTTAGAT CTCCTGATGT TGTTCTTGAA 
GGTGTTTTGA GATTAGAATT TGCGAATGAA AATTCCAAAG AAGATGCCGA AAATAATGTG 
AGTGCAGAGG AGGCAAAGGA AATAAGACAC CTTCGCGATA TCATTGAAAG TGAAGAAAAT 
CAAGGAGACG AAGCCGATGC CTCCGGCACT GGAAAAAAAA CTTTAATGGA TAAATTGATC 
AACTACGAAA GAGGAGAGGC CAAAAAATGC TTAGAAACCC ACACAAGTGA CACCTGTCCC 
AAACCACCAA AACCACCACC CTCCACCGAC AAATATGCCG GCCGCTCCGA CCCACCACGT 
GTCGGACCAT CACGTGACCC CAACGACCCA CATGCTGGTA GTGGCGATGA AGACGAAGAA 
GAAGAAGAAG AAGAAGAAGA GGAGGAGGAA GGCGAAAACA CCACAGTGGA ACACACAGAG 
ACAACCGCAG AGACACAACC AGCAGCACCA ACAGTAACAG TAGACGTCTG CGACACAGTG 
AAAAACGCAC TCGATGACAT GGGCAGTCTC ACACAGGCAT GTAACCAAAA ATATAGCGCA 
CCCAACAGAT ATTGGGGATG GAGGTGTGTC ACACCAAGTG GAAAACCTAG TGACACCGGT 
AGTGTTCCTA CCACTCGTAG TAGTGGTGAC ACCACCACTG GTGGTGAAAG TGGTGCTGAA 
GCCAAAAGTC GTCACAAACG TGAAGCACCA AGTGGTCCCG CCTCCACCGC TTCCGCGTCC 
GTCAACTCGG GCAATACCGC CACCACTGGC AAAGACGGAG CCACCGGTGG TTTGTGTATC 
CCACCCAGGA GGCGAAAACT ATATGTGGGG AAATTGGAAG AGTGGGCGAG TGACGAGGCC 
ACGAAGGCGA AGTCACAAAC GGGTGGTGGA AGTGAGTCAC CAAGTGATGG CAAACCTGCG 
TCACAAAATG ATGGCAAACC TGGTGCATCC ACTTTGTCCG CCGCGTCGAC AGAGCCGTCA 
CAGACGTCTC TCCTCCGCGA CGCCTTCATC AAATCTGCTG CGATTGAAAC TTTTTTCTTA 
TGGGATAGAT ACAAAAAGGA AAACACGAAG ACACAAAGTG TTGGGTCACC ACTAGGTGGA 
GTTGGAGTTC CTGGAGCCGG ACCACAACTA CAAACCCTCC CCTCCATCGA TGGTGCCCTC 
TCCCCTAGTG GTACCCTTGA ACCCAACGAC CCCAATAACA TATATAGTGG AAAAATCCCC 
CCCGATTTCT TGAGATTAAT GTTTTATACA TTAGGAGATT ATAGAGATAT ATGCATAGGT 
GATGACACCA TGATTAAGGC GTTAAAAGCT AGTGGTGATA ACAATATCGA GACAATAAAA 
GAAGCTATAG ATAAAATTTT AAATAGTGGT AACAAAGAAA ATAGTGTCCC CCCACAAACT 
AGTGACAAAA ACCCCGGCCA AACACCACAA AACTGGTGGG ATGATAATGC AAAATATATT 
TGGAAAGGGA TGATATGTGC ACTGACATAT GACACAGATA GTGGCGCAAT AGGACAACCA 
CCACAAGTGG ATCCGCAAGT GAAACAGGCA CTTTTGGATA CAACCGGCAA CAAACCCCAA 
AGCAACAAAT ACCAATACAA CACCGTCACA CTTAAAGATG AAAGTAGTGA GACCCAAGAC 
AAAACCAACG AATCCACCTC CGCAGCCTCT AGTGATACCC CCACCCTTGA CTCCTTCATC 
AAGCGACCCC CCTACTTCCG ATACCTTGAA GAATGGGGTC AAAATTTTTG TAAAGAAAGG 
AAGAAAAGAT TGGAGAAGAT AGAGGAGGAG TGTAAAGTAG AAAATGGTGG TAAAAAACAG 
AACCCAAAAT GTAGTTGTTA TGGGGAGGAT TGTGACAATA TTTTTAGTCA GAAATATAAT 
ATTCTTCCGT CTTTTAATTG TCGTGATTGT GGAAAATATT GTACATTTTA TAAAAGGTGG 
ATAAATGGGA AAAAAACGGA ATATGATGAA CAACAAAAAA TATATAAGAA ACAAAAAGGC 
AACTGTAAAA AGGAAAGTGC GGGTGCTACG GAATTTTGTG GAACACTAGA AAGTCGCCCT 
GAAGCTAAAG ACTTTTTACA AAATTTAGGA TCATGTAAAA AGGATATTGA TAATGGAGAA 
GACAATAAAA TAAATTTTAA TGATAAAGAT AAAACATTTG GACCTTCTCC AAATTGTAAA 
CCATGTTCTC AATTTACTGT TGATTGTAAA AATGGTAAAT GTAAGAGTAG TCTAAATAGG 
AAGTGCCAAG GTAACATTAC TGTTGATACG TTTGATACAA TGGGAGAAGA ACCTCAGGAA 
GTTGTTATGC GTGTCAGTGA TAGCAATACA AATGGAAATA AATTTAATGA TTTAAACGAT 
TGTAAAAATG CAGATATTTT TAAAGGTATT AGAAAAGAAC AATGGAAATG TCGTAATGTA 
TGTGGTTATG TTGTATGTAA ACCGGAAAAC GGTACTGCGG ATACCCATGG TAACAAATAT 
ATACAAATTA GAGCATTGAT GAGACGTTGG GTAGAACATT TTTTAGAAGA TTATAATAGA 
ATTAAACATA AAATTTCACA TTGTACTAAT AATAATGAAC AATCTCCATG TATAAATGGA 
TGTCAAAATA AATGTAATTG TGTGAAAGAA TGGATAGAGA AGAAAAAAGA AGAATGGGGA 
AAAATACAAG AACGTTTCAA TGAGCAATAT AAAAGTGAAA ATTCAGGTGA TACTTTTCCA 
GTGAGAAGTT TTTTGGAGGG CTTGATACCT CAAATTGCTG TTACAGACGT TAAAAATGAG 
GTTATAAAAT TAAGTAAGTT CGGTAATTCT TGTGGATGTA GTACCAGTGC CAGTTCAGAA 
AAGAGTAAAG AGGATCCTAT AGAATGTTTG CTTGACAAGC TTGAAGAAAA AGCAACATCT 
TGTAAAGACA AAAATAGTGA CAAACAAACA AAAGCATCCT GTGAAGAATC CACCCCCCCT 
GATGATGAGG ACTTACTCCT TGAAGAGGAA GAAAACCCAA AAAATATGCG ACCGGGATTT 
TGTCCAACAC CAGAACCAGA ACGAGAACTG GATGAAGGCA AGTGTGAAGA AGCATCATCC 
CCTGTTGTAC CCGAACAACC TGCAAAAGAA GAAAGTGGCG CCCCTCCAGC ACCACCAGCA 
ACAGATAGTG GTAAGGAAAC CAACAACGAA GACACCCCTG CCCCCGAAGC CGGACCCCCC 
GCACCGGCGC CTCCATCAAC ACCAGAATCA CCATTAGCCC CTTCTGACGA ATCCATCCTA 
CATACTACCA TTCCTTTTGG AGTGGCGTTG GCATTAGGAT CCATTGCTTT TTTATTTTTG 
AAGAAAAAAA CCAAATCTAC TATTGATCTT TTGCGTGTCA TTAATATCCC CAAAAGTGAT 
TATAATATAC CGACAAAACT TTCACCCAAT AGATATATAC CTTATACTAG TGGTAAATAC 
AGAGGAAAAC GGTACATTTA CCTTGAAGGA GATAGTGGAA CTGATAGTGG TTACACCGAT 
CATTATAGTG ATATAACTTC ATCTTCCGAA AGTGAATATG AAGAAATGGA TATCAATGAT 
ATATATGTTC CTGGTAGTCC TAAATATAAA ACATTGATCG AAGTAGTACT TGAACCTAGT 
GGTAACAACA CAACAGCTAG TGGTAACAAC ACACCAACTA GTGGTAAAAA CACAACAGCT 
AGTGATACAC AAAATGATAT ACAAAGTGAT GATACACCTA GTAGTAAAAT TACAGATAAT 
GAATGGAATA CATTGAAAGA TGAATTTATA TCACAATATA TACAAAGTGA ACAACCAAAT 
GATTATAAAA GTGGAAATAG TTCAACAAAT ACCAATATTA CTACCACGTC ACATCATAAT 
GTGGAAGAAA AACCTTTTAT TATGTCCATA CATGATAGAA ATTTATATAC TGGAGAAGAA 
TACAATTACG ATATGAGTAA TAATAGTGGT ATATATCCAA GTAGTAGTAA CCGTGATTCA 
TTAAGTGGTA CAAAAGTTCC ATATAGTGGT ATAGATTTAA TTAATGATTC ATTAAATAGT 
GGTAATCAAC CTATTGATAT ATATGATGAA GTTTTGAAAA GAAAAGAAAA CGAATTATTC 
GGGACAGAAC ATCACCCAAA ACGTACAACA ATTAACCGTT TCGCCAAACC TACACGTGAC 
GACCCCATCC ACAACCAACT GGAACTATTC CATAAATGGT TAGATAGACA TAGAGATATG 
TGCGAGAAGT GGAATAATAA AGAGGAACTA TTAGATAAAT TAAAAGAAGA GTGGGAAAAT 
GAGACACATA GTGGTAACAC TCACCCTAGT GATAGTAACA AAACGTTGAA TACTGATGTT 
TCTATTCAAA TACATATGGA TAATCCCAAA ACTACAAATG AGTTTACATA TGTGGATAGT 
AACCCCAACC AAGTGGATGA CACCTACGTG GATAGTAACC CTGACAATTC TTCTATGGAT 
ACTATCTTGG AGGATCTGGA TAAATATAAT GAACCTTATT ATGATGTACA AGATGATATT 
TATTATGATG TACATGATCA TGATACATCA ACTGTGGATA GTAATGCTAT GGATGTTCCT 
AGTAAAGTAC AAATTGAAAT GGATGTAAAT ACGAAATTGG TGAAAGAGAA ATATCCTATA 
GCAGATGTAT GGGATATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002808906.1
CDS	1..6861
Translation	MVNQNGGGGGKDEDAKYMFDRIGQQVHDEVKNGEAKNYVSELEGKLSFASILGETGGFYKTCDIIKNEGEKLLRARDDPCGNGSASDKRFSKESGGECDDKKIEGNKNNSEGGACAPYRRLSLCNKNMEKKGTTKIKDKNDLLAEVCMAANYEAQSLIPYHDKYKLTYGDSKICTALARSFADIGDIVRGKDLYRGGGRGRKQLEENLQKIFGNIYDKLLEENRRNGKNREIEKRYEGDKENYFFKLREDWWTANRATVWEAITCSDRLGGNAYFHATCGESRRPSQAQNQCRCSDKPKAIKAGGNVSIVPTYFDYVPQYLRWFEEWAEDFCRKKKKKVQNLEQQCRGQDISGNQRYCSRNGYDCEKTKPAIGRLRMGKGCTDCFFACNPYIDWINNQRKQFDKQKNKYETEISGGGASGSSRQRRVARGGSNVDGYESKFYKILKDDYETVDGFLELLNKEKTCTKKLKDDDEDEGTINFKTVNSRGGDGSNKTFYRSKYCQPCPLCGVKKNDNGIGWEEKNDSEECKSINLYKPINPDNGTKIEILKSGEGKEEIKKKLEAFCDQINRVTTNAASGGAGGSGSKSDSKELYEEWTCYHVKQLEKENKENGVNDDDYNNDVTTGGGLCILETTNGLNVQKQKTFHNFFYYWVAHMLKDSIYWRTEKLERCLKNGNPMKCRNGCHGKCDCFKRWIDKKEKDEWDKIKEHFGNQEDFKNKGESGEGDMFGRAFRSPDVVLEGVLRLEFANENSKEDAENNVSAEEAKEIRHLRDIIESEENQGDEADASGTGKKTLMDKLINYERGEAKKCLETHTSDTCPKPPKPPPSTDKYAGRSDPPRVGPSRDPNDPHAGSGDEDEEEEEEEEEEEEGENTTVEHTETTAETQPAAPTVTVDVCDTVKNALDDMGSLTQACNQKYSAPNRYWGWRCVTPSGKPSDTGSVPTTRSSGDTTTGGESGAEAKSRHKREAPSGPASTASASVNSGNTATTGKDGATGGLCIPPRRRKLYVGKLEEWASDEATKAKSQTGGGSESPSDGKPASQNDGKPGASTLSAASTEPSQTSLLRDAFIKSAAIETFFLWDRYKKENTKTQSVGSPLGGVGVPGAGPQLQTLPSIDGALSPSGTLEPNDPNNIYSGKIPPDFLRLMFYTLGDYRDICIGDDTMIKALKASGDNNIETIKEAIDKILNSGNKENSVPPQTSDKNPGQTPQNWWDDNAKYIWKGMICALTYDTDSGAIGQPPQVDPQVKQALLDTTGNKPQSNKYQYNTVTLKDESSETQDKTNESTSAASSDTPTLDSFIKRPPYFRYLEEWGQNFCKERKKRLEKIEEECKVENGGKKQNPKCSCYGEDCDNIFSQKYNILPSFNCRDCGKYCTFYKRWINGKKTEYDEQQKIYKKQKGNCKKESAGATEFCGTLESRPEAKDFLQNLGSCKKDIDNGEDNKINFNDKDKTFGPSPNCKPCSQFTVDCKNGKCKSSLNRKCQGNITVDTFDTMGEEPQEVVMRVSDSNTNGNKFNDLNDCKNADIFKGIRKEQWKCRNVCGYVVCKPENGTADTHGNKYIQIRALMRRWVEHFLEDYNRIKHKISHCTNNNEQSPCINGCQNKCNCVKEWIEKKKEEWGKIQERFNEQYKSENSGDTFPVRSFLEGLIPQIAVTDVKNEVIKLSKFGNSCGCSTSASSEKSKEDPIECLLDKLEEKATSCKDKNSDKQTKASCEESTPPDDEDLLLEEEENPKNMRPGFCPTPEPERELDEGKCEEASSPVVPEQPAKEESGAPPAPPATDSGKETNNEDTPAPEAGPPAPAPPSTPESPLAPSDESILHTTIPFGVALALGSIAFLFLKKKTKSTIDLLRVINIPKSDYNIPTKLSPNRYIPYTSGKYRGKRYIYLEGDSGTDSGYTDHYSDITSSSESEYEEMDINDIYVPGSPKYKTLIEVVLEPSGNNTTASGNNTPTSGKNTTASDTQNDIQSDDTPSSKITDNEWNTLKDEFISQYIQSEQPNDYKSGNSSTNTNITTTSHHNVEEKPFIMSIHDRNLYTGEEYNYDMSNNSGIYPSSSNRDSLSGTKVPYSGIDLINDSLNSGNQPIDIYDEVLKRKENELFGTEHHPKRTTINRFAKPTRDDPIHNQLELFHKWLDRHRDMCEKWNNKEELLDKLKEEWENETHSGNTHPSDSNKTLNTDVSIQIHMDNPKTTNEFTYVDSNPNQVDDTYVDSNPDNSSMDTILEDLDKYNEPYYDVQDDIYYDVHDHDTSTVDSNAMDVPSKVQIEMDVNTKLVKEKYPIADVWDI

Target ORF information:

RefSeq Version	XM_002808860.1
Organism	Plasmodium falciparum 3D7
Definition	Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_0700100), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002808860.1

ORF Insert Sequence:

ATGGTGAACC AAAATGGTGG TGGGGGTGGT AAGGATGAAG ATGCCAAATA TATGTTTGAT 
AGGATAGGGC AACAAGTGCA CGACGAAGTG AAGAATGGTG AGGCTAAGAA TTATGTTAGT 
GAATTGGAAG GAAAGTTGTC ATTTGCATCA ATTTTGGGGG AAACAGGTGG CTTTTATAAA 
ACGTGCGACA TTATAAAAAA TGAAGGTGAG AAACTTCTTC GTGCTCGCGA TGATCCGTGC 
GGAAATGGAA GTGCAAGTGA TAAACGTTTT TCTAAAGAAA GTGGCGGTGA ATGTGATGAT 
AAGAAAATAG AAGGTAATAA AAACAATAGT GAAGGTGGAG CCTGTGCTCC ATATAGACGG 
TTATCTCTAT GTAACAAAAA TATGGAAAAA AAGGGAACAA CGAAAATCAA GGATAAAAAT 
GATTTGCTGG CAGAAGTGTG TATGGCAGCA AATTACGAGG CACAGTCATT AATACCTTAT 
CATGACAAAT ATAAATTAAC TTATGGTGAT TCTAAAATAT GTACTGCCCT TGCACGAAGT 
TTTGCGGATA TAGGAGATAT TGTGAGAGGA AAAGATCTGT ATCGTGGTGG TGGTAGGGGA 
AGAAAACAAT TAGAAGAAAA TTTACAAAAA ATTTTTGGGA ATATATATGA CAAATTATTA 
GAGGAGAACC GGAGGAATGG GAAGAACAGG GAGATAGAAA AACGCTACGA AGGTGATAAA 
GAAAATTATT TTTTTAAATT ACGAGAAGAT TGGTGGACTG CGAATCGCGC CACAGTGTGG 
GAAGCTATCA CGTGCAGCGA CAGGCTAGGG GGTAATGCAT ATTTTCATGC AACGTGCGGT 
GAGAGTAGAA GACCATCTCA AGCTCAAAAC CAATGCCGCT GTAGCGACAA GCCAAAGGCC 
ATCAAGGCAG GTGGCAACGT AAGTATTGTC CCCACATATT TTGACTACGT GCCGCAGTAT 
CTTCGGTGGT TCGAGGAATG GGCAGAAGAT TTTTGTAGGA AAAAAAAAAA AAAAGTACAA 
AATTTGGAAC AACAATGTCG TGGACAAGAT ATAAGTGGTA ACCAACGATA TTGTAGCCGT 
AATGGCTACG ATTGCGAAAA AACTAAACCA GCGATTGGTA GATTGCGTAT GGGTAAAGGT 
TGCACTGACT GTTTTTTTGC ATGTAATCCT TACATTGATT GGATAAATAA CCAACGAAAA 
CAATTTGACA AACAAAAAAA CAAATACGAA ACAGAAATAT CAGGTGGTGG TGCTAGTGGT 
AGTAGTAGGC AAAGACGAGT TGCACGTGGT GGTAGTAATG TTGATGGATA TGAAAGTAAA 
TTTTATAAAA TACTTAAAGA CGACTATGAA ACCGTTGATG GTTTTTTGGA ATTATTAAAT 
AAAGAAAAAA CATGCACAAA AAAATTGAAG GATGACGACG AAGACGAAGG AACAATTAAT 
TTTAAAACCG TTAATAGTAG AGGTGGTGAT GGTAGTAATA AAACGTTTTA TCGTTCAAAA 
TATTGCCAAC CCTGTCCTCT ATGTGGAGTG AAAAAGAATG ATAATGGTAT TGGATGGGAA 
GAGAAAAATG ATAGTGAAGA ATGCAAGAGT ATAAACCTTT ATAAGCCTAT AAACCCAGAT 
AACGGTACTA AGATTGAAAT CCTTAAAAGT GGTGAAGGAA AAGAAGAAAT AAAAAAAAAA 
TTAGAGGCGT TTTGCGATCA AATAAATCGT GTTACAACAA ATGCTGCTAG TGGTGGCGCT 
GGTGGTAGTG GTAGTAAAAG TGATAGTAAG GAACTGTATG AAGAATGGAC ATGTTATCAC 
GTTAAACAGC TGGAGAAAGA AAATAAAGAA AATGGTGTGA ATGACGACGA CTATAACAAC 
GATGTAACAA CTGGAGGCGG ATTATGTATA TTGGAAACGA CGAACGGCTT AAATGTGCAA 
AAACAAAAGA CATTCCATAA TTTTTTTTAC TATTGGGTAG CACATATGTT AAAAGATTCC 
ATATATTGGA GAACAGAAAA ACTTGAGAGA TGTTTAAAAA ATGGTAATCC TATGAAATGT 
AGAAATGGAT GCCATGGTAA ATGTGATTGT TTTAAAAGAT GGATTGATAA AAAAGAAAAA 
GACGAATGGG ACAAAATAAA AGAACATTTT GGCAACCAAG AAGATTTTAA GAATAAAGGA 
GAAAGTGGCG AAGGTGACAT GTTTGGCAGA GCATTTAGAT CTCCTGATGT TGTTCTTGAA 
GGTGTTTTGA GATTAGAATT TGCGAATGAA AATTCCAAAG AAGATGCCGA AAATAATGTG 
AGTGCAGAGG AGGCAAAGGA AATAAGACAC CTTCGCGATA TCATTGAAAG TGAAGAAAAT 
CAAGGAGACG AAGCCGATGC CTCCGGCACT GGAAAAAAAA CTTTAATGGA TAAATTGATC 
AACTACGAAA GAGGAGAGGC CAAAAAATGC TTAGAAACCC ACACAAGTGA CACCTGTCCC 
AAACCACCAA AACCACCACC CTCCACCGAC AAATATGCCG GCCGCTCCGA CCCACCACGT 
GTCGGACCAT CACGTGACCC CAACGACCCA CATGCTGGTA GTGGCGATGA AGACGAAGAA 
GAAGAAGAAG AAGAAGAAGA GGAGGAGGAA GGCGAAAACA CCACAGTGGA ACACACAGAG 
ACAACCGCAG AGACACAACC AGCAGCACCA ACAGTAACAG TAGACGTCTG CGACACAGTG 
AAAAACGCAC TCGATGACAT GGGCAGTCTC ACACAGGCAT GTAACCAAAA ATATAGCGCA 
CCCAACAGAT ATTGGGGATG GAGGTGTGTC ACACCAAGTG GAAAACCTAG TGACACCGGT 
AGTGTTCCTA CCACTCGTAG TAGTGGTGAC ACCACCACTG GTGGTGAAAG TGGTGCTGAA 
GCCAAAAGTC GTCACAAACG TGAAGCACCA AGTGGTCCCG CCTCCACCGC TTCCGCGTCC 
GTCAACTCGG GCAATACCGC CACCACTGGC AAAGACGGAG CCACCGGTGG TTTGTGTATC 
CCACCCAGGA GGCGAAAACT ATATGTGGGG AAATTGGAAG AGTGGGCGAG TGACGAGGCC 
ACGAAGGCGA AGTCACAAAC GGGTGGTGGA AGTGAGTCAC CAAGTGATGG CAAACCTGCG 
TCACAAAATG ATGGCAAACC TGGTGCATCC ACTTTGTCCG CCGCGTCGAC AGAGCCGTCA 
CAGACGTCTC TCCTCCGCGA CGCCTTCATC AAATCTGCTG CGATTGAAAC TTTTTTCTTA 
TGGGATAGAT ACAAAAAGGA AAACACGAAG ACACAAAGTG TTGGGTCACC ACTAGGTGGA 
GTTGGAGTTC CTGGAGCCGG ACCACAACTA CAAACCCTCC CCTCCATCGA TGGTGCCCTC 
TCCCCTAGTG GTACCCTTGA ACCCAACGAC CCCAATAACA TATATAGTGG AAAAATCCCC 
CCCGATTTCT TGAGATTAAT GTTTTATACA TTAGGAGATT ATAGAGATAT ATGCATAGGT 
GATGACACCA TGATTAAGGC GTTAAAAGCT AGTGGTGATA ACAATATCGA GACAATAAAA 
GAAGCTATAG ATAAAATTTT AAATAGTGGT AACAAAGAAA ATAGTGTCCC CCCACAAACT 
AGTGACAAAA ACCCCGGCCA AACACCACAA AACTGGTGGG ATGATAATGC AAAATATATT 
TGGAAAGGGA TGATATGTGC ACTGACATAT GACACAGATA GTGGCGCAAT AGGACAACCA 
CCACAAGTGG ATCCGCAAGT GAAACAGGCA CTTTTGGATA CAACCGGCAA CAAACCCCAA 
AGCAACAAAT ACCAATACAA CACCGTCACA CTTAAAGATG AAAGTAGTGA GACCCAAGAC 
AAAACCAACG AATCCACCTC CGCAGCCTCT AGTGATACCC CCACCCTTGA CTCCTTCATC 
AAGCGACCCC CCTACTTCCG ATACCTTGAA GAATGGGGTC AAAATTTTTG TAAAGAAAGG 
AAGAAAAGAT TGGAGAAGAT AGAGGAGGAG TGTAAAGTAG AAAATGGTGG TAAAAAACAG 
AACCCAAAAT GTAGTTGTTA TGGGGAGGAT TGTGACAATA TTTTTAGTCA GAAATATAAT 
ATTCTTCCGT CTTTTAATTG TCGTGATTGT GGAAAATATT GTACATTTTA TAAAAGGTGG 
ATAAATGGGA AAAAAACGGA ATATGATGAA CAACAAAAAA TATATAAGAA ACAAAAAGGC 
AACTGTAAAA AGGAAAGTGC GGGTGCTACG GAATTTTGTG GAACACTAGA AAGTCGCCCT 
GAAGCTAAAG ACTTTTTACA AAATTTAGGA TCATGTAAAA AGGATATTGA TAATGGAGAA 
GACAATAAAA TAAATTTTAA TGATAAAGAT AAAACATTTG GACCTTCTCC AAATTGTAAA 
CCATGTTCTC AATTTACTGT TGATTGTAAA AATGGTAAAT GTAAGAGTAG TCTAAATAGG 
AAGTGCCAAG GTAACATTAC TGTTGATACG TTTGATACAA TGGGAGAAGA ACCTCAGGAA 
GTTGTTATGC GTGTCAGTGA TAGCAATACA AATGGAAATA AATTTAATGA TTTAAACGAT 
TGTAAAAATG CAGATATTTT TAAAGGTATT AGAAAAGAAC AATGGAAATG TCGTAATGTA 
TGTGGTTATG TTGTATGTAA ACCGGAAAAC GGTACTGCGG ATACCCATGG TAACAAATAT 
ATACAAATTA GAGCATTGAT GAGACGTTGG GTAGAACATT TTTTAGAAGA TTATAATAGA 
ATTAAACATA AAATTTCACA TTGTACTAAT AATAATGAAC AATCTCCATG TATAAATGGA 
TGTCAAAATA AATGTAATTG TGTGAAAGAA TGGATAGAGA AGAAAAAAGA AGAATGGGGA 
AAAATACAAG AACGTTTCAA TGAGCAATAT AAAAGTGAAA ATTCAGGTGA TACTTTTCCA 
GTGAGAAGTT TTTTGGAGGG CTTGATACCT CAAATTGCTG TTACAGACGT TAAAAATGAG 
GTTATAAAAT TAAGTAAGTT CGGTAATTCT TGTGGATGTA GTACCAGTGC CAGTTCAGAA 
AAGAGTAAAG AGGATCCTAT AGAATGTTTG CTTGACAAGC TTGAAGAAAA AGCAACATCT 
TGTAAAGACA AAAATAGTGA CAAACAAACA AAAGCATCCT GTGAAGAATC CACCCCCCCT 
GATGATGAGG ACTTACTCCT TGAAGAGGAA GAAAACCCAA AAAATATGCG ACCGGGATTT 
TGTCCAACAC CAGAACCAGA ACGAGAACTG GATGAAGGCA AGTGTGAAGA AGCATCATCC 
CCTGTTGTAC CCGAACAACC TGCAAAAGAA GAAAGTGGCG CCCCTCCAGC ACCACCAGCA 
ACAGATAGTG GTAAGGAAAC CAACAACGAA GACACCCCTG CCCCCGAAGC CGGACCCCCC 
GCACCGGCGC CTCCATCAAC ACCAGAATCA CCATTAGCCC CTTCTGACGA ATCCATCCTA 
CATACTACCA TTCCTTTTGG AGTGGCGTTG GCATTAGGAT CCATTGCTTT TTTATTTTTG 
AAGAAAAAAA CCAAATCTAC TATTGATCTT TTGCGTGTCA TTAATATCCC CAAAAGTGAT 
TATAATATAC CGACAAAACT TTCACCCAAT AGATATATAC CTTATACTAG TGGTAAATAC 
AGAGGAAAAC GGTACATTTA CCTTGAAGGA GATAGTGGAA CTGATAGTGG TTACACCGAT 
CATTATAGTG ATATAACTTC ATCTTCCGAA AGTGAATATG AAGAAATGGA TATCAATGAT 
ATATATGTTC CTGGTAGTCC TAAATATAAA ACATTGATCG AAGTAGTACT TGAACCTAGT 
GGTAACAACA CAACAGCTAG TGGTAACAAC ACACCAACTA GTGGTAAAAA CACAACAGCT 
AGTGATACAC AAAATGATAT ACAAAGTGAT GATACACCTA GTAGTAAAAT TACAGATAAT 
GAATGGAATA CATTGAAAGA TGAATTTATA TCACAATATA TACAAAGTGA ACAACCAAAT 
GATTATAAAA GTGGAAATAG TTCAACAAAT ACCAATATTA CTACCACGTC ACATCATAAT 
GTGGAAGAAA AACCTTTTAT TATGTCCATA CATGATAGAA ATTTATATAC TGGAGAAGAA 
TACAATTACG ATATGAGTAA TAATAGTGGT ATATATCCAA GTAGTAGTAA CCGTGATTCA 
TTAAGTGGTA CAAAAGTTCC ATATAGTGGT ATAGATTTAA TTAATGATTC ATTAAATAGT 
GGTAATCAAC CTATTGATAT ATATGATGAA GTTTTGAAAA GAAAAGAAAA CGAATTATTC 
GGGACAGAAC ATCACCCAAA ACGTACAACA ATTAACCGTT TCGCCAAACC TACACGTGAC 
GACCCCATCC ACAACCAACT GGAACTATTC CATAAATGGT TAGATAGACA TAGAGATATG 
TGCGAGAAGT GGAATAATAA AGAGGAACTA TTAGATAAAT TAAAAGAAGA GTGGGAAAAT 
GAGACACATA GTGGTAACAC TCACCCTAGT GATAGTAACA AAACGTTGAA TACTGATGTT 
TCTATTCAAA TACATATGGA TAATCCCAAA ACTACAAATG AGTTTACATA TGTGGATAGT 
AACCCCAACC AAGTGGATGA CACCTACGTG GATAGTAACC CTGACAATTC TTCTATGGAT 
ACTATCTTGG AGGATCTGGA TAAATATAAT GAACCTTATT ATGATGTACA AGATGATATT 
TATTATGATG TACATGATCA TGATACATCA ACTGTGGATA GTAATGCTAT GGATGTTCCT 
AGTAAAGTAC AAATTGAAAT GGATGTAAAT ACGAAATTGG TGAAAGAGAA ATATCCTATA 
GCAGATGTAT GGGATATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PF3D7_0700100 cDNA ORF clone, Plasmodium falciparum 3D7

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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