ORF » Species Summary » Leishmania major strain Friedlin » LMJF_30_0510 cDNA ORF clone

LMJF_30_0510 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_30_0510 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_30_0510 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb04162 XM_001684589.1
Latest version!		 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_30_0510 ( XM_001684589.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001684641.1 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.
    Price & Availability↑
    CloneID OLb04162
    Clone ID Related Accession (Same CDS sequence) XM_001684589.1
    Accession Version XM_001684589.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product conserved hypothetical protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007271). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGCGCGA GCCCCTCAGC GACGGTGCAC GTGCCCACCG TGTACGACTC GATGCATGAG 
    CAGTCCCGCA GCATCGGAAC GTTCGCCTCT ACAGAGCTGG AGAACGTCTA CAGCTTGTCG 
    GCACGCATGT CAGCGGCGCG CCATGCCACG TCGCCTTCCT CCGCCACGCG CAGCCTCGCG 
    GACTTCCTCG ACGACATCTT CTTCTCCTAC ACCGGGGTGC CATCGTGGTC AACCTTTCTC 
    CACATCCAGC AGGAGGGGGC GGCGCTGCGG CGCCAGCTCG GCGACGGCGC CACTTTTGCA 
    CACGAAGCCG TGCACCCGTC GCTATCGCCC GTTCTGAAGC AGTGGGCTCA GACACTTCGA 
    AGCCAGCGGG CGGCGCGTGC ACGCGCCATT GGCGCCGGCG ACTCTCCTCG CAACCCCATA 
    GTCGTGAGCG CTGCCGCTCC GCGCGGCGCG ACGTCGACAG GAAAGCCACC CTTCAGATCT 
    GAGTTGAGCG CCTCGGTGAC TGCTGCTGCT CCTGCTGCCA CATTGAACTA CAATGCAACG 
    AAGTCCGTGG CTTTCATGAC CGCCGGCACC TCCGATGATC TAGATAGCTC CTCCTCGCTG 
    ATGGAGCGGG GGTTGGCGGC GGAGAACGCA CGCGCGCACC GCCCAGCCGA GGAGGACGGT 
    GTGCTACCTT TATGGAAGGA CTTGAAGCCG ACAAAGCGCT GCGGCCCGCT TGCGCTGCAG 
    GGCTTGCGAG CGGCTGTCGG CGCCTACTAC CTGGTGCTAC TGCGCCTCGT GGACATTACC 
    GAGTCGCTGG CCGATCATTG GGGGCTTCTC TCGCCCAAGG AGCTGAAGGG GCTGCACCGT 
    GCCATGGCCG CTGAAAAGGC TGCACACGTG CCGCCTAGCT TTCTCTTCTC TGTCTTCACG 
    GAGCTGAACG CGTCGGGGGA GCGCCTGAAC AACGTCGTGG CGGATTTCTG CAAGTGCACT 
    CGTCTGGAGA CGGTTCAGCT CAACGGCAAC GCAGCGCTGA CGGAGCTGAG CGTGCTGCCG 
    CCTCACTGCC GCGTCGTGAC CGCGTGCGGA TGCAGCCTTG ATCGCTTTCT CGAGGAGGCG 
    CGTTCGACGC CGCCATCGCC GCCCTCGGGG GCGCCGCGTG CCGTATACGC ATCGGTGACG 
    ACACTGGGGC TCGCCTATAA CTGCCTGCGG CACCTACACT TCTTACAGCA GCTACCCTCT 
    CTGCGCGTTC TGAACATGTC CTTCAATTAC GTGAGTGATT TGGAGGCTGC GGTGCGGGAT 
    GTGGCCGCGC ACGGGTCACT GACGGAAGTG ACGCTGCAAG GCAACCCTAT TTCGCTTTTG 
    GATGCTTATC GCACTGTCGT GGTGCGTGGC TGCGTGCACC TCGACCGACT CGACGGGGTC 
    ACCGTCACCG GGGAGGAGCG AGCCTTGAGC TGCCTGACCG TCGACGGCGG GGACGCCGCT 
    CAGGAGGACT CGCCGGGGCG GCCTTTGCTC GACAGCGCAC TTGCCGGTGC TGAGAGGAAG 
    CCATCTTGTA CGGCGCTGCC TGACATCTGC GGACGGCGTC GATTGACCGT GTCCGCCTCT 
    CCGGCCTCGC CTTCGCAGCA GCACAGCCCG TCGACACGAG GCTGCGTGAC CTTCGGAGCT 
    CTTCCCATCC CCAAATCGAA CTCGGTAGTG GCCATGGTGG ACTCGCAGCC ACCTGTGCCA 
    GGTGAGGTAC TGCGGACAAC CGTGGCGGCG GGGCTGGATT TGGTCATGGT GAGAGGTCTG 
    GCCAGTCTCC AGCCCGCGCC ACAGACTTGC CTGGCAGATA GCTTGCTGCC CTCCTCCGGC 
    TTCTTACTTG AGTTAGCAGG AGTAGATACG TCGGCGCAGG CATCGGTGCC GAGGGGTGGC 
    TGCACCTCGC CTATACAGGC AAGCTCCCTC GTCACGTCAC CGCTGGCATC CCCTCGCACT 
    TCCTCTGTGC AGGCAGGCAG CCGCAAGGGC CGCCACCACG GCGGGGCGGC CGGGGACAGC 
    GGTGGCAGAG GCGCCTCTGC CGACTGCAAT TCCACAAGCG GAAAGCGAGT CGTGCCGCCG 
    CTGTACGAGG TGTCGTCACG GGTCACGGTG GAGGGCTGCT GGGGCGGCTC TAGCGTCTCA 
    AGCGGCGATG CGCCACTCGG CAGCGCAGAC GGTGTGCGTG TTGCTGTGGA AGTATGTCTG 
    GAGCCACCCG CTCCCTTATG CACGCACTCG GCGGGCCGGC ATGCTGGAGC ACTTGGTGCG 
    CCACCACCGC GCGTGTCGTC TGCCGCACAA AGTAGGCAGC GCAGGACTGG CGGGACCGCG 
    GCTGCCGCCA ACGGGGAAAC ACCGCCACTC CCCATCCCCG ACCCGTACAT CCCGGCCCGC 
    TGCACGGCGG GGGCCGGAGG TGGCGGCGGA GCTTCATGCG TAGTGAGCCT CCCCGTCACC 
    GACACCCTCG TCTGCGCTCT TCAGCAGCCG CTAGTGCTGC GCGTCATGGT GCAGGACACA 
    TTCCGGTTCG CAGAGGGTGA GGCTGCCCTG CGCTCGCAGC TGGACACCTC TGCGTCAAGC 
    GCGGCCCGCG GCAATCCGGT AGGTCGAAGC GGCGCTGCTG CGCGGAGTGT CCCTGGGGAG 
    CTTCCATCGA ACGTAGCGCT AACCTCTGCC TTCTCGCTAG GGGGGGCACC GCCGTGGGCA 
    ACATCGTGCC AACGCGGAAG CGAGGATGCC GAGGAGGAGG AGGCAGTGCT GCTCCTGCGA 
    CGCGAGCTCG GGGTGATAAC GCTGGACCCC CGCAATCTTT TCATGGCGGC TGACGGCAAT 
    CCAGCGACGT GTACGGGGGC GCCATGCGCG CCCACTCCGC TTCCCCGCTG CCGTGTGTTG 
    TATGTGCACG ATGCGGTTCT CGAAAGAGAC TCGCACACGC TGAAAAGCGC AGAGCGCGAG 
    GTGCGGCAGT GTCAGCGTCG CCTCCGTGAG GCTCTTGCGA CCTACTCGGA CATGCACCAC 
    CAGTACAAGG AGGCAAGCCG CAAGGGCTTG GAAAACCCCG ACTCTCTCTC CCCGGCGCCG 
    TCAGCAGCAC TCGGTAAGGA CAGCGTCGCC ACTGGCACGA CGTCACCGCA GTCGAACAGA 
    CGGCGCACCT CCGTAGCGGT AAACTCCTCT ACGGTGGTGC TGCCGCTGTC ATCCGCGTCG 
    TCGAACAAGC AATCCGCCCG CTCCCGCCCC GCGAGTGTGG TGGCCCCGCC TGCCCACACT 
    TCACAGTTGC AGGGTTTGTA TGCCAGCCTC AAAGCTGAGC AACTGCGCGT GGCTCAGCGA 
    GCCTTACGTG TGCTGGCGCT ACGGACACGA CTGGACGAGC TCAGCGGCGC GTCTCTCTCC 
    GTCAGCGTTC GGTTTTGCGT TGGGCGTGGC GCGGCCCCGC CGCCGTCGAC CGCCGCCGAT 
    GCCGAACTGG AGGCGTTGCG CCACCCCACC GGGTCACCCA AGGGTCGCAA AGGACGCCAG 
    CAGGCCGCCA AAGGAACTCG TCGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001684641.1
    CDS1..3447
    Translation

    Target ORF information:

    RefSeq Version XM_001684589.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001684589.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGCGCGA GCCCCTCAGC GACGGTGCAC GTGCCCACCG TGTACGACTC GATGCATGAG 
    CAGTCCCGCA GCATCGGAAC GTTCGCCTCT ACAGAGCTGG AGAACGTCTA CAGCTTGTCG 
    GCACGCATGT CAGCGGCGCG CCATGCCACG TCGCCTTCCT CCGCCACGCG CAGCCTCGCG 
    GACTTCCTCG ACGACATCTT CTTCTCCTAC ACCGGGGTGC CATCGTGGTC AACCTTTCTC 
    CACATCCAGC AGGAGGGGGC GGCGCTGCGG CGCCAGCTCG GCGACGGCGC CACTTTTGCA 
    CACGAAGCCG TGCACCCGTC GCTATCGCCC GTTCTGAAGC AGTGGGCTCA GACACTTCGA 
    AGCCAGCGGG CGGCGCGTGC ACGCGCCATT GGCGCCGGCG ACTCTCCTCG CAACCCCATA 
    GTCGTGAGCG CTGCCGCTCC GCGCGGCGCG ACGTCGACAG GAAAGCCACC CTTCAGATCT 
    GAGTTGAGCG CCTCGGTGAC TGCTGCTGCT CCTGCTGCCA CATTGAACTA CAATGCAACG 
    AAGTCCGTGG CTTTCATGAC CGCCGGCACC TCCGATGATC TAGATAGCTC CTCCTCGCTG 
    ATGGAGCGGG GGTTGGCGGC GGAGAACGCA CGCGCGCACC GCCCAGCCGA GGAGGACGGT 
    GTGCTACCTT TATGGAAGGA CTTGAAGCCG ACAAAGCGCT GCGGCCCGCT TGCGCTGCAG 
    GGCTTGCGAG CGGCTGTCGG CGCCTACTAC CTGGTGCTAC TGCGCCTCGT GGACATTACC 
    GAGTCGCTGG CCGATCATTG GGGGCTTCTC TCGCCCAAGG AGCTGAAGGG GCTGCACCGT 
    GCCATGGCCG CTGAAAAGGC TGCACACGTG CCGCCTAGCT TTCTCTTCTC TGTCTTCACG 
    GAGCTGAACG CGTCGGGGGA GCGCCTGAAC AACGTCGTGG CGGATTTCTG CAAGTGCACT 
    CGTCTGGAGA CGGTTCAGCT CAACGGCAAC GCAGCGCTGA CGGAGCTGAG CGTGCTGCCG 
    CCTCACTGCC GCGTCGTGAC CGCGTGCGGA TGCAGCCTTG ATCGCTTTCT CGAGGAGGCG 
    CGTTCGACGC CGCCATCGCC GCCCTCGGGG GCGCCGCGTG CCGTATACGC ATCGGTGACG 
    ACACTGGGGC TCGCCTATAA CTGCCTGCGG CACCTACACT TCTTACAGCA GCTACCCTCT 
    CTGCGCGTTC TGAACATGTC CTTCAATTAC GTGAGTGATT TGGAGGCTGC GGTGCGGGAT 
    GTGGCCGCGC ACGGGTCACT GACGGAAGTG ACGCTGCAAG GCAACCCTAT TTCGCTTTTG 
    GATGCTTATC GCACTGTCGT GGTGCGTGGC TGCGTGCACC TCGACCGACT CGACGGGGTC 
    ACCGTCACCG GGGAGGAGCG AGCCTTGAGC TGCCTGACCG TCGACGGCGG GGACGCCGCT 
    CAGGAGGACT CGCCGGGGCG GCCTTTGCTC GACAGCGCAC TTGCCGGTGC TGAGAGGAAG 
    CCATCTTGTA CGGCGCTGCC TGACATCTGC GGACGGCGTC GATTGACCGT GTCCGCCTCT 
    CCGGCCTCGC CTTCGCAGCA GCACAGCCCG TCGACACGAG GCTGCGTGAC CTTCGGAGCT 
    CTTCCCATCC CCAAATCGAA CTCGGTAGTG GCCATGGTGG ACTCGCAGCC ACCTGTGCCA 
    GGTGAGGTAC TGCGGACAAC CGTGGCGGCG GGGCTGGATT TGGTCATGGT GAGAGGTCTG 
    GCCAGTCTCC AGCCCGCGCC ACAGACTTGC CTGGCAGATA GCTTGCTGCC CTCCTCCGGC 
    TTCTTACTTG AGTTAGCAGG AGTAGATACG TCGGCGCAGG CATCGGTGCC GAGGGGTGGC 
    TGCACCTCGC CTATACAGGC AAGCTCCCTC GTCACGTCAC CGCTGGCATC CCCTCGCACT 
    TCCTCTGTGC AGGCAGGCAG CCGCAAGGGC CGCCACCACG GCGGGGCGGC CGGGGACAGC 
    GGTGGCAGAG GCGCCTCTGC CGACTGCAAT TCCACAAGCG GAAAGCGAGT CGTGCCGCCG 
    CTGTACGAGG TGTCGTCACG GGTCACGGTG GAGGGCTGCT GGGGCGGCTC TAGCGTCTCA 
    AGCGGCGATG CGCCACTCGG CAGCGCAGAC GGTGTGCGTG TTGCTGTGGA AGTATGTCTG 
    GAGCCACCCG CTCCCTTATG CACGCACTCG GCGGGCCGGC ATGCTGGAGC ACTTGGTGCG 
    CCACCACCGC GCGTGTCGTC TGCCGCACAA AGTAGGCAGC GCAGGACTGG CGGGACCGCG 
    GCTGCCGCCA ACGGGGAAAC ACCGCCACTC CCCATCCCCG ACCCGTACAT CCCGGCCCGC 
    TGCACGGCGG GGGCCGGAGG TGGCGGCGGA GCTTCATGCG TAGTGAGCCT CCCCGTCACC 
    GACACCCTCG TCTGCGCTCT TCAGCAGCCG CTAGTGCTGC GCGTCATGGT GCAGGACACA 
    TTCCGGTTCG CAGAGGGTGA GGCTGCCCTG CGCTCGCAGC TGGACACCTC TGCGTCAAGC 
    GCGGCCCGCG GCAATCCGGT AGGTCGAAGC GGCGCTGCTG CGCGGAGTGT CCCTGGGGAG 
    CTTCCATCGA ACGTAGCGCT AACCTCTGCC TTCTCGCTAG GGGGGGCACC GCCGTGGGCA 
    ACATCGTGCC AACGCGGAAG CGAGGATGCC GAGGAGGAGG AGGCAGTGCT GCTCCTGCGA 
    CGCGAGCTCG GGGTGATAAC GCTGGACCCC CGCAATCTTT TCATGGCGGC TGACGGCAAT 
    CCAGCGACGT GTACGGGGGC GCCATGCGCG CCCACTCCGC TTCCCCGCTG CCGTGTGTTG 
    TATGTGCACG ATGCGGTTCT CGAAAGAGAC TCGCACACGC TGAAAAGCGC AGAGCGCGAG 
    GTGCGGCAGT GTCAGCGTCG CCTCCGTGAG GCTCTTGCGA CCTACTCGGA CATGCACCAC 
    CAGTACAAGG AGGCAAGCCG CAAGGGCTTG GAAAACCCCG ACTCTCTCTC CCCGGCGCCG 
    TCAGCAGCAC TCGGTAAGGA CAGCGTCGCC ACTGGCACGA CGTCACCGCA GTCGAACAGA 
    CGGCGCACCT CCGTAGCGGT AAACTCCTCT ACGGTGGTGC TGCCGCTGTC ATCCGCGTCG 
    TCGAACAAGC AATCCGCCCG CTCCCGCCCC GCGAGTGTGG TGGCCCCGCC TGCCCACACT 
    TCACAGTTGC AGGGTTTGTA TGCCAGCCTC AAAGCTGAGC AACTGCGCGT GGCTCAGCGA 
    GCCTTACGTG TGCTGGCGCT ACGGACACGA CTGGACGAGC TCAGCGGCGC GTCTCTCTCC 
    GTCAGCGTTC GGTTTTGCGT TGGGCGTGGC GCGGCCCCGC CGCCGTCGAC CGCCGCCGAT 
    GCCGAACTGG AGGCGTTGCG CCACCCCACC GGGTCACCCA AGGGTCGCAA AGGACGCCAG 
    CAGGCCGCCA AAGGAACTCG TCGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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