mdc1 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	mdc1
Entrez Gene ID	105416732
Full Name	mediator of DNA damage checkpoint 1
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011605922.2	XP_011604224.2	mediator of DNA damage checkpoint protein 1
XM_011605922.1	XP_011604224.1	mediator of DNA damage checkpoint protein 1

Pathways from BioSystems
Gene Ontology

The following mdc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mdc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo32832	XM_011605922.1 Latest version!	Takifugu rubripes mediator of DNA-damage checkpoint 1 (mdc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo69194	XM_011605922.2 Latest version!	Takifugu rubripes mediator of DNA damage checkpoint protein 1 (LOC105416732), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mdc1 ( XM_011605922.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011604224.1 Takifugu rubripes mediator of DNA-damage checkpoint 1 (mdc1), mRNA.

Price & Availability↑

CloneID	OTo32832
Clone ID Related Accession (Same CDS sequence)	XM_011605922.1
Accession Version	XM_011605922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070991.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA CTCAGATGAT AAATGACTCT GTGCTGGAGT CAGATGAGGA AGAAAATGAA 
GCTAATCAAC CCAGCAAGGG TAGACCGGTC GCTAAACTAC TCATTTTAAA AAATGATCAC 
ATGCCTGAGA CAGAATTACC ACTCTTTTTG GGAGAAAATG TTTTGGGCCG GGATGGTAAC 
ACTTGCACTG TGCCCTTGCC ATCTCCCTCC ATTTCCAAGC AGCATGCAAC CGTCTCAATC 
TCCTTGTATC GATCTAGAGG AGAATCAGAT CTGGAGGCTT TGGTTTGGGA TCTCGGGAGC 
CTGAATGGGA CCCGCAAAGG TCGTCTTAAG TTGACTCCAA ATGTACGCTA TGCCCTCGTT 
GCCCAGGACA GTTTGGTTTT GGCAGATATC CCGTGTCAGT TTGTCAGTTG TTCTGCAGAC 
ACAGTCTCTG CTCAGCATAA CTTCCAGACA CCTGTGAGTC GAAGCTCCCT GGCAGATGCC 
TCCAATGAAA AGGGAAGTGG CACCGATGTT GGTGGTGAGA AATCTGAAAG AGTTTCAGAT 
GCAAAGGTCT CATCACCAGT TCTCAAAGAC ACAAAGAATA CCCCCATGCG GACCATTTGC 
CTGTCCTTTG AGCAAACTCC AACTCAGCCA GAGGGCACCC TGGTCCCAGA ATCTGAATCA 
GATGAAGAGA GAGAGGGTCG AGCCAATCGG AGGCAGAAGA CTTTAGATTC TGATTCCGAC 
CTCAATAAAT CTAGTCCTAC CTGTTCAAAC TTCCTGACTC CCACAAACAA AACTGTCCCT 
GAAAGTGAGG ATGAAAGTCC TATTACACCA CCCTTCTCCA CTAAAAATAG GCCTCACAGA 
CATGTCGGAT TCAACAAAGG AGAGACAAAT ATAAATATGG GCCGACAGCA GCTGAAGGAA 
AAACCGGCAT CCGAAAAAGA GGAAGAACAT TTTTCCACGG ATAGTGGCCC TCATATGCAA 
GAGAATCAGG AAACCAATGC CCAGCTCAAA TATGATAACC TGCCTGTTGC TGCACCATCA 
GTCACCACAG ACGCATTTCA AATGGACAGT GACACTGATT TGGAGGGAGA AGAGGACGGG 
GTGGCTCCGA CAGCCCCTGC GACCTTGACT ACAAACCAAA TTGTTGATGG CACATCAGAC 
AAAGCCCAGT TTTGCATGGA GAGTGATACC GATGTTGAGG AGGACGCTCA CGCCTCGCGC 
AAAGTTCCCG GACCTGCGTC TCTGCCTGAA AACAACACAA AACCTTTACC TGAAACTCCA 
GTCTTTCGGC CAGAGGGCGT CACTGTGGAC AGTGATACGG ACGTGGATGA TGACGACACA 
TTGGGCGCTG CGTCAAAAGC TAAACCCACT TCAGTCCAGA GCAGAACCAC TGCTGACTCC 
ACTCCAACAA CACACCTGAA ACAGTTCCAT TCTGACAGTG ACGCAGAGAG CGAGGAGGAC 
GACATGAAAC GAGTGCCAAG TAATTCATCC TTTAAAATAA GCGAGACACC CGCTAAATTA 
GCCAAAGGTG TTTCTGCTGT GCCGCCCTGC CCAGGCAGTG AGACGCATGA TGACGCCCTT 
CCTGCCATCA GGAAATCTGG TGCCGCGGAA TCGTGGAATG CTGCAGACAC ACGTGCTGAT 
TTGGATATTC TGTCTGACAG CGACACGGAT GCGGAACATG ATTCTCCTCT GGTAAAACAA 
ACATTTGTTG GCACAAATAT GTCACTCACA CGCTGTCCTG TGTCAGAAGC CATCCAGTCA 
GATTCTGACG CAGACACAGA TGTGGAAGAA TCCACCACAG CCCCCGTTTT GGGGAGGGTA 
ACTACAGCCA GCCTTCAGGA AGGAGGAGAA AAGGATGTGG AAGTTGAGGT CAATGTTGCG 
GCGCCTGGAG AAGGTCACGT CCCGCGCCTC GTCAGGGAGG AGACTCCTGG ACTCTTGAAT 
CCTTCCTACC AGCACTGCTC TACTCCTGTC CAGTTGCCAG AAAATCAAGT GGAGGATATG 
GAGACCCAGG CCTTCCTGAG CCCCACCTCA GTGAGATATA CGGGAGCTCC TGCTCTGAGG 
ACTCAAGCAT TAGATTTGGA CTCAGACAGT CAGGAGGAGG AGAACATCGT TGTGGCCGAG 
ACACAGTCTT TTGTTCTGCA GACCCGAGCC TGTCGGGGCA GTCGCTCGGA GGGCCACATC 
AGAGAAGCCA CGCAGACATC CGCCCTCGAG TCTGCTGATG ATGAAATGAG CAAACAGTCC 
TGCAGAGAAG AGTCTTTTCA GCTGGGATTG TCTGAGAGCA GCCACATTCA GGATCCAGCT 
GCAGCTGCAG ACATGGAAAG CACCCAGGCA TTTGTCTCTG TAGAGGCCAC CCAACTATAT 
GCAGCCACCT CATCGACGAG TGCTTCAGAA CTCCATGCTG CACCAGCTGA TGGCGATCAG 
GGGGAGGAGC CAGCCACAGG CTCAGCTGTA CCTGAAAGGG AAGGTTGGGA AAATTTGGCC 
TTAGAAGCAA CACAGGCTTA CCTTTCAGAC CCATATCATG ATTCAGAAGA GGAAACGGAT 
GATGAGGAGA CTCAGCCCTT TCATGGTCCA GCCTCTGCTA CCCTCGCCAT GGCTGAAACT 
CAACCAATGT GTGCTTTTGA GGAGGAGGAC ATAAAGCATC CTGTTTCCAC TGCAGTGCAA 
GTTAAGGTCA GAGCAAAGAA TGAAACAGAA GAGAGGGCAG AGCACAGTGA GGCGGCCGTA 
GCTCAGGGGA GGCCCCTCAA GGTGGATCTG TCTCTAGCCG AAACTCAGCC CATGTGCACC 
AGCGATGACA GGGAAAGTGA TGATGAGGAT TCGTTTCCAG GTCTACGAAA AGGGAAAACA 
AAACAGCTGC AGATTGAAGA TGAGCAGACG CAGCCCCTCA CAAGTTCAGA GCAATCCGCT 
GTTGAAACGC AGCCGCTGCA GACTGTTGAC CAGGACCTGG CTCCAGGGGG AAGCGAGGCC 
TTTCATCAAG ATAATCCAGT TGCAGCTCCA CGCAGAGAAG TGCTGCAATT TAAAGAGGAA 
AAGCCAGCAG CTCTAATTAG CTCTGATCTG TCCACTGCTG AAACGCAGCT CATGACCACA 
GGTGACGATG AAGAAAGTGA CGAAGCTGAC GCGGTTTTAG CTCTTCCAAG AAGGAAAGCA 
AAACCTCTGC AGCACCAAGA AGATGAGACA CAGCCGGCTG TTTGTGAGCC TTCGGTTTTC 
CAAAGCCGGC CACTGATTTC AGGAGAAAAC CACGATGGCG TTGGTTCACT TCCTGGTACA 
GAAATGACAA AAGTAAAGCC TTCACAAATC TTAGAAGAGA GGATACAGCT GCTCACAAAT 
TCGACGTCCT CCACACACGA AACTGGCGTG TTGGAAACCA AAGCTGACGC TGAGGCTGGA 
ACCAGTGGAA GCAAAGTTAA TGGTAGAAGA GGAACAAGGG CAAATTCGAG AAAGGTGTCA 
ACACAGTGCG ATGAACCTTC CAGGAGATCG ACAAGAGGGA TAAAAGATTC TTCAGCTGCA 
GCCCATAGAG GAACATCAAA GTCTGGCGAA GAAAAGGATG AGGAGCTGGA GGATAAGGAA 
GAGGAGCATA ACAGCGAAGC TGAGGGAATT CAATCCAGAA ATGATGTGGA CGTTTTGAAC 
AAGACTGAAC AGAACATTAG TCAAATCCAG AAACAACAAG AACCGAGTCT GGGAGAGCAG 
AGAGGTGGCA CTGAGCCAAG ACAACACGAA AACATGGAGA AGGAAAGGAA GGAAACAGAA 
GAAAACAAAA GAACTGAGAC TGAACCTTCA AACAGGCTTG AATGGGAGAG AGCAGAAAAA 
GAGCAAACAG TTTCAACAAA GAAAAAAGAC AGGGAAGACA AAGAGAGATT AGAGCACAAC 
AAGGCAGAAA GGGAGGAGAA GGAGAGATTG GAAAAAGAGA AGAATGTTCT GATGGCCAAC 
AGACAAACAG AACAAGAAAA AGAAATGTTG GAGAGTGAAA GGAGGAAACG TGATGAGAAC 
CTTGGGAGGG AAGATCAACA ACGCAGTGCA AGACAGGACA GTGAAGCAAA GGGGGCAGAC 
AGAATAGAAG CCTCTGAGAA AGAACAGCTA ACGGAGGAAA AGACAGTAGA AAAACAAGAG 
CAGAAGGAAG AAAACAAGGC CAAAGTGTCT GCAAGAGGAC GAAGAGCAGC CCAGAGAACC 
GCCGTGTGTA AAACAGAGCC AGTAACAGAC ACGTGTGTCT CCACTAATGA CGACGTCCCA 
GCGAGGAGAA CAAGATCTCG CTCCAACTCC TCCAACTCGG TCAGCTCAGA GAGGTCCGCT 
TTGAGCATTA ACCTGCAGGA GGGCGGACGG AGAGGAGCAA GGAGGACCAG TGAGCCGCCC 
CGCGTGGTTG CTTCCAGAAG CAGCACCAGG AGGAAGACGG TGGCTGCACG TCCAACACAA 
GGGGACCGTA ACGATGATAC TGGCATAACG GGGGCCCAGG GCCAAGCACG AGGAAGCAGG 
CAGCGCACGA GAGGAGGAAA AGCTGAGGCA GTTTCTCCTG CGGTCAGTCA GAGTGACCCC 
AAAACTGTAA CCAGAGGCAG GAAGAACACT AAAGCCCAGT CCTCCAACGA TGCCGAGAAG 
GCAGGTTCTC AGCAGGCTAC TACCTCTAGG GGGCAGCGTC GATCTAGTTC AAATGGCTCT 
GGACCCACAG AAGCTAAAGG CTCCTCGAAT CAAGGCGAGC TCATTGGCAA TGAGGAAACG 
CTTCAGTCTA AAAAGAATGT AAGGGGCAGA AGCCATAAAC CAGCAAAAAG GGAGGCAGTG 
GTGGCCCCAC AATCCCCTGC TGTCAGTAGT CCAGGTGAGA CAAAAGAGCT TAGAAAAGGA 
AGAAAAAGAG GGTCTGAAGC AAATACTGAC ATTGACTCGG ACAGAAGTTC CAAATTCATG 
AAAGGGGGGG AAATAGAACA GACACCAGGA GAAGCTGAAG AAGCACCTAA ACAAGCACAA 
GATGAGACTC CTGCACCAGC TAAGAGAAGA AGTAGAGCGT CCGTCGCTCA GACGAAGAAA 
GCTCTCGATC CGTCTGGACC TGGGCTGAAG GAAACTAATG AAAGAATAGC CGAGGAGCCC 
GTGGAGAAGA GAGGCCGAGG TCGACTGGCA GTGGGCCAGA AACAGAAAAG AGAGGAGCAG 
GAAGGTGCCG AAGTGCCTGT CGAGCAGCGT GCACGTGTGC TGGAAGCAGA GGTCCAAACC 
CCAGCCAGCA GTGGGTCCAG GAAGCGCCAG GCTCCTGTGG ATGCTTCGCC CTTCAGGAAG 
ACCCCTCGCG CCTCCTCCCT GTCCCCAGCA GCTCACGTTC GCTCGCGTGC CGTCGGCCAC 
GCGTACAAGG TGCTGTTCAC AGGTGTGGTG GACGAAGCAG GGGAGCAAGT GTTGGCCCGT 
TTGGGAGGCA GCGTGGCCAA AGGCGTGGCC GACATGAACT GTCTGGTGAC CGATAAGCTG 
CGCCGGACCG TGAAGCTCCT GTGTGCCGTG GCCAAAGGAA TCCCTGTTGT TACCACGCTG 
TGGCTGGAAA AGAGTGGGAA GACTGGGAGC TTCCTGTCTC CCAGTTCCTT CATTGTGAAG 
GATCTGGAGC AGGAGAAGAA ATTCAATTTC TGCTTGCAGG AGTCTCTCAA GGTTGCCAGC 
AGCCAGCCGC TCTTACAGGG GTACGAAATT CATGTTACCA AGTCGGTGAA GCCTGAGCCA 
GTTCAGATGA GAGACATACT CACATGCAGC GGCGCTACCT TCCTCCCCAA GATGCCCAGT 
TCTCACAAGG CTCAAACTGT AGTGATTTCA TGTGAAGAGG ACTGGCACTT ATGTGCTCGC 
GCCGCGTCGG CGTCAGTCCC CGTGGTCACC GCCGAGTTCA TCCTCGCCGG GATCCTGCAG 
CAGAAACTGG ACTTTCAGAG CCACGCGCTC TCCCCCCCTG CAGGCCGCCT GCAGCCTGTA 
GGCAGAGCGA GGCAACGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604224.1
CDS	128..6031
Translation	MDATQMINDSVLESDEEENEANQPSKGRPVAKLLILKNDHMPETELPLFLGENVLGRDGNTCTVPLPSPSISKQHATVSISLYRSRGESDLEALVWDLGSLNGTRKGRLKLTPNVRYALVAQDSLVLADIPCQFVSCSADTVSAQHNFQTPVSRSSLADASNEKGSGTDVGGEKSERVSDAKVSSPVLKDTKNTPMRTICLSFEQTPTQPEGTLVPESESDEEREGRANRRQKTLDSDSDLNKSSPTCSNFLTPTNKTVPESEDESPITPPFSTKNRPHRHVGFNKGETNINMGRQQLKEKPASEKEEEHFSTDSGPHMQENQETNAQLKYDNLPVAAPSVTTDAFQMDSDTDLEGEEDGVAPTAPATLTTNQIVDGTSDKAQFCMESDTDVEEDAHASRKVPGPASLPENNTKPLPETPVFRPEGVTVDSDTDVDDDDTLGAASKAKPTSVQSRTTADSTPTTHLKQFHSDSDAESEEDDMKRVPSNSSFKISETPAKLAKGVSAVPPCPGSETHDDALPAIRKSGAAESWNAADTRADLDILSDSDTDAEHDSPLVKQTFVGTNMSLTRCPVSEAIQSDSDADTDVEESTTAPVLGRVTTASLQEGGEKDVEVEVNVAAPGEGHVPRLVREETPGLLNPSYQHCSTPVQLPENQVEDMETQAFLSPTSVRYTGAPALRTQALDLDSDSQEEENIVVAETQSFVLQTRACRGSRSEGHIREATQTSALESADDEMSKQSCREESFQLGLSESSHIQDPAAAADMESTQAFVSVEATQLYAATSSTSASELHAAPADGDQGEEPATGSAVPEREGWENLALEATQAYLSDPYHDSEEETDDEETQPFHGPASATLAMAETQPMCAFEEEDIKHPVSTAVQVKVRAKNETEERAEHSEAAVAQGRPLKVDLSLAETQPMCTSDDRESDDEDSFPGLRKGKTKQLQIEDEQTQPLTSSEQSAVETQPLQTVDQDLAPGGSEAFHQDNPVAAPRREVLQFKEEKPAALISSDLSTAETQLMTTGDDEESDEADAVLALPRRKAKPLQHQEDETQPAVCEPSVFQSRPLISGENHDGVGSLPGTEMTKVKPSQILEERIQLLTNSTSSTHETGVLETKADAEAGTSGSKVNGRRGTRANSRKVSTQCDEPSRRSTRGIKDSSAAAHRGTSKSGEEKDEELEDKEEEHNSEAEGIQSRNDVDVLNKTEQNISQIQKQQEPSLGEQRGGTEPRQHENMEKERKETEENKRTETEPSNRLEWERAEKEQTVSTKKKDREDKERLEHNKAEREEKERLEKEKNVLMANRQTEQEKEMLESERRKRDENLGREDQQRSARQDSEAKGADRIEASEKEQLTEEKTVEKQEQKEENKAKVSARGRRAAQRTAVCKTEPVTDTCVSTNDDVPARRTRSRSNSSNSVSSERSALSINLQEGGRRGARRTSEPPRVVASRSSTRRKTVAARPTQGDRNDDTGITGAQGQARGSRQRTRGGKAEAVSPAVSQSDPKTVTRGRKNTKAQSSNDAEKAGSQQATTSRGQRRSSSNGSGPTEAKGSSNQGELIGNEETLQSKKNVRGRSHKPAKREAVVAPQSPAVSSPGETKELRKGRKRGSEANTDIDSDRSSKFMKGGEIEQTPGEAEEAPKQAQDETPAPAKRRSRASVAQTKKALDPSGPGLKETNERIAEEPVEKRGRGRLAVGQKQKREEQEGAEVPVEQRARVLEAEVQTPASSGSRKRQAPVDASPFRKTPRASSLSPAAHVRSRAVGHAYKVLFTGVVDEAGEQVLARLGGSVAKGVADMNCLVTDKLRRTVKLLCAVAKGIPVVTTLWLEKSGKTGSFLSPSSFIVKDLEQEKKFNFCLQESLKVASSQPLLQGYEIHVTKSVKPEPVQMRDILTCSGATFLPKMPSSHKAQTVVISCEEDWHLCARAASASVPVVTAEFILAGILQQKLDFQSHALSPPAGRLQPVGRARQRT

Target ORF information:

RefSeq Version	XM_011605922.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mediator of DNA-damage checkpoint 1 (mdc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605922.1

ORF Insert Sequence:

ATGGATGCAA CTCAGATGAT AAATGACTCT GTGCTGGAGT CAGATGAGGA AGAAAATGAA 
GCTAATCAAC CCAGCAAGGG TAGACCGGTC GCTAAACTAC TCATTTTAAA AAATGATCAC 
ATGCCTGAGA CAGAATTACC ACTCTTTTTG GGAGAAAATG TTTTGGGCCG GGATGGTAAC 
ACTTGCACTG TGCCCTTGCC ATCTCCCTCC ATTTCCAAGC AGCATGCAAC CGTCTCAATC 
TCCTTGTATC GATCTAGAGG AGAATCAGAT CTGGAGGCTT TGGTTTGGGA TCTCGGGAGC 
CTGAATGGGA CCCGCAAAGG TCGTCTTAAG TTGACTCCAA ATGTACGCTA TGCCCTCGTT 
GCCCAGGACA GTTTGGTTTT GGCAGATATC CCGTGTCAGT TTGTCAGTTG TTCTGCAGAC 
ACAGTCTCTG CTCAGCATAA CTTCCAGACA CCTGTGAGTC GAAGCTCCCT GGCAGATGCC 
TCCAATGAAA AGGGAAGTGG CACCGATGTT GGTGGTGAGA AATCTGAAAG AGTTTCAGAT 
GCAAAGGTCT CATCACCAGT TCTCAAAGAC ACAAAGAATA CCCCCATGCG GACCATTTGC 
CTGTCCTTTG AGCAAACTCC AACTCAGCCA GAGGGCACCC TGGTCCCAGA ATCTGAATCA 
GATGAAGAGA GAGAGGGTCG AGCCAATCGG AGGCAGAAGA CTTTAGATTC TGATTCCGAC 
CTCAATAAAT CTAGTCCTAC CTGTTCAAAC TTCCTGACTC CCACAAACAA AACTGTCCCT 
GAAAGTGAGG ATGAAAGTCC TATTACACCA CCCTTCTCCA CTAAAAATAG GCCTCACAGA 
CATGTCGGAT TCAACAAAGG AGAGACAAAT ATAAATATGG GCCGACAGCA GCTGAAGGAA 
AAACCGGCAT CCGAAAAAGA GGAAGAACAT TTTTCCACGG ATAGTGGCCC TCATATGCAA 
GAGAATCAGG AAACCAATGC CCAGCTCAAA TATGATAACC TGCCTGTTGC TGCACCATCA 
GTCACCACAG ACGCATTTCA AATGGACAGT GACACTGATT TGGAGGGAGA AGAGGACGGG 
GTGGCTCCGA CAGCCCCTGC GACCTTGACT ACAAACCAAA TTGTTGATGG CACATCAGAC 
AAAGCCCAGT TTTGCATGGA GAGTGATACC GATGTTGAGG AGGACGCTCA CGCCTCGCGC 
AAAGTTCCCG GACCTGCGTC TCTGCCTGAA AACAACACAA AACCTTTACC TGAAACTCCA 
GTCTTTCGGC CAGAGGGCGT CACTGTGGAC AGTGATACGG ACGTGGATGA TGACGACACA 
TTGGGCGCTG CGTCAAAAGC TAAACCCACT TCAGTCCAGA GCAGAACCAC TGCTGACTCC 
ACTCCAACAA CACACCTGAA ACAGTTCCAT TCTGACAGTG ACGCAGAGAG CGAGGAGGAC 
GACATGAAAC GAGTGCCAAG TAATTCATCC TTTAAAATAA GCGAGACACC CGCTAAATTA 
GCCAAAGGTG TTTCTGCTGT GCCGCCCTGC CCAGGCAGTG AGACGCATGA TGACGCCCTT 
CCTGCCATCA GGAAATCTGG TGCCGCGGAA TCGTGGAATG CTGCAGACAC ACGTGCTGAT 
TTGGATATTC TGTCTGACAG CGACACGGAT GCGGAACATG ATTCTCCTCT GGTAAAACAA 
ACATTTGTTG GCACAAATAT GTCACTCACA CGCTGTCCTG TGTCAGAAGC CATCCAGTCA 
GATTCTGACG CAGACACAGA TGTGGAAGAA TCCACCACAG CCCCCGTTTT GGGGAGGGTA 
ACTACAGCCA GCCTTCAGGA AGGAGGAGAA AAGGATGTGG AAGTTGAGGT CAATGTTGCG 
GCGCCTGGAG AAGGTCACGT CCCGCGCCTC GTCAGGGAGG AGACTCCTGG ACTCTTGAAT 
CCTTCCTACC AGCACTGCTC TACTCCTGTC CAGTTGCCAG AAAATCAAGT GGAGGATATG 
GAGACCCAGG CCTTCCTGAG CCCCACCTCA GTGAGATATA CGGGAGCTCC TGCTCTGAGG 
ACTCAAGCAT TAGATTTGGA CTCAGACAGT CAGGAGGAGG AGAACATCGT TGTGGCCGAG 
ACACAGTCTT TTGTTCTGCA GACCCGAGCC TGTCGGGGCA GTCGCTCGGA GGGCCACATC 
AGAGAAGCCA CGCAGACATC CGCCCTCGAG TCTGCTGATG ATGAAATGAG CAAACAGTCC 
TGCAGAGAAG AGTCTTTTCA GCTGGGATTG TCTGAGAGCA GCCACATTCA GGATCCAGCT 
GCAGCTGCAG ACATGGAAAG CACCCAGGCA TTTGTCTCTG TAGAGGCCAC CCAACTATAT 
GCAGCCACCT CATCGACGAG TGCTTCAGAA CTCCATGCTG CACCAGCTGA TGGCGATCAG 
GGGGAGGAGC CAGCCACAGG CTCAGCTGTA CCTGAAAGGG AAGGTTGGGA AAATTTGGCC 
TTAGAAGCAA CACAGGCTTA CCTTTCAGAC CCATATCATG ATTCAGAAGA GGAAACGGAT 
GATGAGGAGA CTCAGCCCTT TCATGGTCCA GCCTCTGCTA CCCTCGCCAT GGCTGAAACT 
CAACCAATGT GTGCTTTTGA GGAGGAGGAC ATAAAGCATC CTGTTTCCAC TGCAGTGCAA 
GTTAAGGTCA GAGCAAAGAA TGAAACAGAA GAGAGGGCAG AGCACAGTGA GGCGGCCGTA 
GCTCAGGGGA GGCCCCTCAA GGTGGATCTG TCTCTAGCCG AAACTCAGCC CATGTGCACC 
AGCGATGACA GGGAAAGTGA TGATGAGGAT TCGTTTCCAG GTCTACGAAA AGGGAAAACA 
AAACAGCTGC AGATTGAAGA TGAGCAGACG CAGCCCCTCA CAAGTTCAGA GCAATCCGCT 
GTTGAAACGC AGCCGCTGCA GACTGTTGAC CAGGACCTGG CTCCAGGGGG AAGCGAGGCC 
TTTCATCAAG ATAATCCAGT TGCAGCTCCA CGCAGAGAAG TGCTGCAATT TAAAGAGGAA 
AAGCCAGCAG CTCTAATTAG CTCTGATCTG TCCACTGCTG AAACGCAGCT CATGACCACA 
GGTGACGATG AAGAAAGTGA CGAAGCTGAC GCGGTTTTAG CTCTTCCAAG AAGGAAAGCA 
AAACCTCTGC AGCACCAAGA AGATGAGACA CAGCCGGCTG TTTGTGAGCC TTCGGTTTTC 
CAAAGCCGGC CACTGATTTC AGGAGAAAAC CACGATGGCG TTGGTTCACT TCCTGGTACA 
GAAATGACAA AAGTAAAGCC TTCACAAATC TTAGAAGAGA GGATACAGCT GCTCACAAAT 
TCGACGTCCT CCACACACGA AACTGGCGTG TTGGAAACCA AAGCTGACGC TGAGGCTGGA 
ACCAGTGGAA GCAAAGTTAA TGGTAGAAGA GGAACAAGGG CAAATTCGAG AAAGGTGTCA 
ACACAGTGCG ATGAACCTTC CAGGAGATCG ACAAGAGGGA TAAAAGATTC TTCAGCTGCA 
GCCCATAGAG GAACATCAAA GTCTGGCGAA GAAAAGGATG AGGAGCTGGA GGATAAGGAA 
GAGGAGCATA ACAGCGAAGC TGAGGGAATT CAATCCAGAA ATGATGTGGA CGTTTTGAAC 
AAGACTGAAC AGAACATTAG TCAAATCCAG AAACAACAAG AACCGAGTCT GGGAGAGCAG 
AGAGGTGGCA CTGAGCCAAG ACAACACGAA AACATGGAGA AGGAAAGGAA GGAAACAGAA 
GAAAACAAAA GAACTGAGAC TGAACCTTCA AACAGGCTTG AATGGGAGAG AGCAGAAAAA 
GAGCAAACAG TTTCAACAAA GAAAAAAGAC AGGGAAGACA AAGAGAGATT AGAGCACAAC 
AAGGCAGAAA GGGAGGAGAA GGAGAGATTG GAAAAAGAGA AGAATGTTCT GATGGCCAAC 
AGACAAACAG AACAAGAAAA AGAAATGTTG GAGAGTGAAA GGAGGAAACG TGATGAGAAC 
CTTGGGAGGG AAGATCAACA ACGCAGTGCA AGACAGGACA GTGAAGCAAA GGGGGCAGAC 
AGAATAGAAG CCTCTGAGAA AGAACAGCTA ACGGAGGAAA AGACAGTAGA AAAACAAGAG 
CAGAAGGAAG AAAACAAGGC CAAAGTGTCT GCAAGAGGAC GAAGAGCAGC CCAGAGAACC 
GCCGTGTGTA AAACAGAGCC AGTAACAGAC ACGTGTGTCT CCACTAATGA CGACGTCCCA 
GCGAGGAGAA CAAGATCTCG CTCCAACTCC TCCAACTCGG TCAGCTCAGA GAGGTCCGCT 
TTGAGCATTA ACCTGCAGGA GGGCGGACGG AGAGGAGCAA GGAGGACCAG TGAGCCGCCC 
CGCGTGGTTG CTTCCAGAAG CAGCACCAGG AGGAAGACGG TGGCTGCACG TCCAACACAA 
GGGGACCGTA ACGATGATAC TGGCATAACG GGGGCCCAGG GCCAAGCACG AGGAAGCAGG 
CAGCGCACGA GAGGAGGAAA AGCTGAGGCA GTTTCTCCTG CGGTCAGTCA GAGTGACCCC 
AAAACTGTAA CCAGAGGCAG GAAGAACACT AAAGCCCAGT CCTCCAACGA TGCCGAGAAG 
GCAGGTTCTC AGCAGGCTAC TACCTCTAGG GGGCAGCGTC GATCTAGTTC AAATGGCTCT 
GGACCCACAG AAGCTAAAGG CTCCTCGAAT CAAGGCGAGC TCATTGGCAA TGAGGAAACG 
CTTCAGTCTA AAAAGAATGT AAGGGGCAGA AGCCATAAAC CAGCAAAAAG GGAGGCAGTG 
GTGGCCCCAC AATCCCCTGC TGTCAGTAGT CCAGGTGAGA CAAAAGAGCT TAGAAAAGGA 
AGAAAAAGAG GGTCTGAAGC AAATACTGAC ATTGACTCGG ACAGAAGTTC CAAATTCATG 
AAAGGGGGGG AAATAGAACA GACACCAGGA GAAGCTGAAG AAGCACCTAA ACAAGCACAA 
GATGAGACTC CTGCACCAGC TAAGAGAAGA AGTAGAGCGT CCGTCGCTCA GACGAAGAAA 
GCTCTCGATC CGTCTGGACC TGGGCTGAAG GAAACTAATG AAAGAATAGC CGAGGAGCCC 
GTGGAGAAGA GAGGCCGAGG TCGACTGGCA GTGGGCCAGA AACAGAAAAG AGAGGAGCAG 
GAAGGTGCCG AAGTGCCTGT CGAGCAGCGT GCACGTGTGC TGGAAGCAGA GGTCCAAACC 
CCAGCCAGCA GTGGGTCCAG GAAGCGCCAG GCTCCTGTGG ATGCTTCGCC CTTCAGGAAG 
ACCCCTCGCG CCTCCTCCCT GTCCCCAGCA GCTCACGTTC GCTCGCGTGC CGTCGGCCAC 
GCGTACAAGG TGCTGTTCAC AGGTGTGGTG GACGAAGCAG GGGAGCAAGT GTTGGCCCGT 
TTGGGAGGCA GCGTGGCCAA AGGCGTGGCC GACATGAACT GTCTGGTGAC CGATAAGCTG 
CGCCGGACCG TGAAGCTCCT GTGTGCCGTG GCCAAAGGAA TCCCTGTTGT TACCACGCTG 
TGGCTGGAAA AGAGTGGGAA GACTGGGAGC TTCCTGTCTC CCAGTTCCTT CATTGTGAAG 
GATCTGGAGC AGGAGAAGAA ATTCAATTTC TGCTTGCAGG AGTCTCTCAA GGTTGCCAGC 
AGCCAGCCGC TCTTACAGGG GTACGAAATT CATGTTACCA AGTCGGTGAA GCCTGAGCCA 
GTTCAGATGA GAGACATACT CACATGCAGC GGCGCTACCT TCCTCCCCAA GATGCCCAGT 
TCTCACAAGG CTCAAACTGT AGTGATTTCA TGTGAAGAGG ACTGGCACTT ATGTGCTCGC 
GCCGCGTCGG CGTCAGTCCC CGTGGTCACC GCCGAGTTCA TCCTCGCCGG GATCCTGCAG 
CAGAAACTGG ACTTTCAGAG CCACGCGCTC TCCCCCCCTG CAGGCCGCCT GCAGCCTGTA 
GGCAGAGCGA GGCAACGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdc1 ( XM_011605922.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011604224.2 Takifugu rubripes mediator of DNA damage checkpoint protein 1 (LOC105416732), mRNA.

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CloneID	OTo69194
Clone ID Related Accession (Same CDS sequence)	XM_011605922.2
Accession Version	XM_011605922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011605922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA CTCAGATGAT AAATGACTCT GTGCTGGAGT CAGATGAGGA AGAAAATGAA 
GCTAATCAAC CCAGCAAGGG TAGACCGGTC GCTAAACTAC TCATTTTAAA AAATGATCAC 
ATGCCTGAGA CAGAATTACC ACTCTTTTTG GGAGAAAATG TTTTGGGCCG GGATGGTAAC 
ACTTGCACTG TGCCCTTGCC ATCTCCCTCC ATTTCCAAGC AGCATGCAAC CGTCTCAATC 
TCCTTGTATC GATCTAGAGG AGAATCAGAT CTGGAGGCTT TGGTTTGGGA TCTCGGGAGC 
CTGAATGGGA CCCGCAAAGG TCGCCTTAAG TTGACTCCAA ATGTACGCTA TGCCCTCGTT 
GCCCAGGACA GTTTGGTTTT GGCAGATATC CCGTGTCAGT TTGTCAGTTG TTCTGCAGAC 
ACAGTCTCTG CTCAGCATAA CTTCCAGACA CCTGTGAGTC GAAGCTCCCT GGCAGATGCC 
TCCAATGAAA AGGGAAGTGG CACCGATGTT GGTGGTGAGA AATCTGAAAG AGTTTCAGAT 
GCAAAGGTCT CATCACCAGT TCTCAAAGAC ACAAAGAATA CCCCCATGCG GACCATTTGC 
CTGTCCTTTG AGCAAACTCC AACTCAGCCA GAGGGCACCC TGGTCCCAGA ATCTGAATCA 
GATGAAGAGA GAGAGGGTCG AGCCAATCGG AGGCAGAAGA CTTTAGATTC TGATTCCGAC 
CTCAATAAAT CTAGTCCTAC CTGTTCAAAC TTCCTGACTC CCACAAACAA AACTGTCCCT 
GAAAGTGAGG ATGAAAGTCC TATTACACCA CCCTTCTCCA CTAAAAATAG GCCTCACAGA 
CATGTCGGAT TCAACAAAGG AGAGACAAAT ATAAATATGG GCCGACAGCA GCTGAAGGAA 
AAACCGGCAT CCGAAAAAGA GGAAGAACAT TTTTCCATGG ATAGTGGCCC TCGTATGCAA 
GAGAATCAGG AAACCAATGC CCAGCTCAAA TACGATAACC TGCCTGTTGC TGCACCATCA 
GTCACCACAG ACGCATTTCA AATGGACAGT GACACTGATT TGGAGGGAGA GGAGGACGGG 
GTGGCGACCT TGACTACAAA CCAAATTGTT GATGGCACAT CAGACAAAGC CCAGTTTTGC 
ATGGAGAGTG ATACGGATGT TGAGGAGGAC GCTCACGCCT CGCGCAAAGT TCCCGGACCT 
GCGTCTCTGC CTGAAAACAA CACAAAACCT TTACCTGAAA CTCCAGTCTT TCGGCCTGTG 
GACAGTGATA CGGACGTGGA TGATGACGAC ACATTGGGCG CTGCGTCAAA AGCTAAACCC 
ACTTCAGTCC AGAGCAGAAC CACTGCTGAC TCCACTCCAA CAACACACCT GAAACAGTTC 
CATTCTGACA GTGACGCAGA GAGCGAGGAG GACGACATGA AACGAGTGCC AAGTAATTCA 
TCCTTTAAAA TAAGCGAGAC ACCCGCTAAA TTAGCCAAAG GTGTTTCTGC TGTGCCGCCC 
TGCCCAGGCA GTGAGACGCA TGATGACGCC CTTCCTGCCA TCAGGAAATC TGGTGCCGCG 
GAATCGTGGA ATGCTGCAGA CACACGTGCT GATTTGGATA TTCTGTCTGA CAGCGACACG 
GATGCGGAAC ATGATTCTCC TCTGGTAAAA CAAACATTTG TTGGCACAAA TATGTCACTC 
ACACGCTGTC CTGTGTCAGA AGCCATCCAG TCAGATTCTG ACGCAGACAC AGATGTGGAA 
GAATCCACCA CAGCCCCCGT TTTGGGGAGG GTAACTACAG CCAGCCTTCA GGAAGGAGGA 
GAAAAGGATG TGGAAGTTGA GGTCAATGTT GCGGCGCCTG GAGAAGGTCA CGTCCCGCGC 
CTCGTCAGGG AGGAGACTCC TGGACTCTTG AATCCTTCCT ACCAGCACTG CTCTACTCCT 
GTCCAGTTGC CAGAAAATCA AGTGGAGGAT ATGGAGACCC AGGCCTTCCT GAGCCCCACC 
TCAGTGAGAT ATACGGGAGC TCCTGCTCTG AGGACTCAAG CATTAGATTT GGACTCAGAC 
AGTCAGGAGG AGGAGAACAT CGTTGTGGCC GAGACACAGT CTTTTGTTCT GCAGACCCGA 
GCCTGTCGGG GCAGTCGCTC GGAGGGCCAC ATCAGAGAAG CCACGCAGAC ATCCGCCCTC 
GAGTCTGCTG ATGATGAAAT GAGCAAACAG TCCTGCAGAG AAGAGTCTTT TCAGCTGGGA 
TTGTCTGAGA GCAGCCACAT TCAGGATCCA GCTGCAGCTG CAGACATGGA AAGCACCCAG 
GCATTTGTCT CTGTAGAGGC CACCCAACTA TATGCAGCCA CCTCATCGAC GAGTGCTTCA 
GAACTCCATG CTGCACCAGC TGATGGCGAT CAGGGGGAGG AGCCAGCCAC AGGCTCAGCT 
GTACCTGAAA GGGAAGGTTG GGAAAATTTG GCCTTAGAAG CAACACAGGC TTACCTTTCA 
GACCCATATC ATGATTCAGA AGAGGAAACG GATGATGAGG AGACTCAGCC CTTTCATGGT 
CCAGCCTCTG CTACCCTCGC CATGGCTGAA ACTCAACCAA TGTGTGCTTT TGAGGAGGAG 
GACATAAAGC ATCCTGTTTC CACTGCAGTG CAAGTTAAGG TCAGAGCAAA GAATGAAACA 
GAAGAGAGGG CAGAGCACAG TGAGGCGGCC GTAGCTCAGG GGAGGCCCCT CAAGGTGGAT 
CTGTCTCTAG CCGAAACTCA GCCCATGTGC ACCAGCGATG ACAGGGAAAG TGATGATGAG 
GATTCGTTTC CAGGTCTACG AAAAGGGAAA ACAAAACAGC TGCAGATTGA AGATGAGCAG 
ACGCAGCCCC TCACAAGTTC AGAGCAATCC GCTGTTGAAA CGCAGCCGCT GCAGACTGTT 
GACCAGGACC TGGCTGCAGG GGGAAGCGAG GCCTTTCATC AAGACAATCC AGTTGCAGCT 
CCACGCAGAG AAGTGCTGCA ATTTAAAGAG GAAAAGCCAG CAGCTCTAAT TAGCTCTGAT 
CTGTCCACTG CTGAAACGCA GCTCATGACC ACAGGTGACG ATGAAGAAAG TGACGAAGCT 
GACGCGGTTT TAGCTCTTCC AAGAAGGAAA GCAAAACCTC TGCAGCACCA AGAAGATGAG 
ACACAGCCGG CTGTTTGTGA GCCTTCGGTT TTCCAAAGCC GGCCACTGAT TTCAGGAGAA 
AACCACGATG GCGTTGGTTC ACTTCCTGGT ACAGAAATGA CAAAAGTAAA GCCTTCACAA 
ATCTTAGAAG AGAGGATACA GCTGCTCACA AATTCGACGT CCTCCACACA CGAAACTGGC 
GTGTTGGAAA CCAAAGCTGA CGCTGAGGCT GGAACCAGTG GAAGCAAAGT TAATGGTAGA 
AGAGGAACGA GGGCAAATTT GAGAAAGGTG TCAACACAGT GCGATGAACC TTCCAGGAGA 
CCGACAAGAG GGATAAAAGA TTCTTCAGCT GCAGCCCGTA GAGGAACATC AAAGTCTGGC 
GAAGAAAAGG ATGAGGAGCT GGAGGATAAG GAAGAGGAGC ATAACAGCGA AGCTGAGGGA 
ATTCAATCCA GAAATGATGT GGACGTTTTG AACAAGACTG AACAGAACAT TAGTCAAATC 
CAGAAACAAC AAGAACCGAG TCTGGGAGAG CAGAGAGGTG GCACTGAGCC AAGACAACAC 
GAAAACATGG AGAAGGAAAG GAAGGAAACA GAAGAAAACA AAAGAACTGA GACTGAACCT 
TCAAACAGGC TTGAATGGGA GAGAGCAGAA AAAGAGCAAA CAGTTTCAAC AAAGAAAAAA 
GACAGGGAAG ACAAAGAGAG ATTAGAGCAC GACAAGGCAG AAAGGGAGGA GAAGGAGAGA 
TTGGAAAAAG AGAAGAATGT TCTGATGGCC AACAGACAAA CAGAACAAGA AAAAGAAATG 
TTGGAGAGTG AAAGGAGGAA ACGTGATGAG AACCTTGGGA GGGAAGATCA ACAACGCAGT 
GCAAGACAGG ACAGTGAAGC AAAGGGGGCA GACAGAATAG AAGCCTCTGA GAAAGAACAG 
CTAACGGAGG AAAAGACAGT AGAAAAACAA GAGCAGAAGG AAGAAAACAA GGCCAAAGTG 
TCTGCAAGAG GACGAAGAGC AGCCCAGAGA ACCGCCGTGT GTAAAACAGA GCCAGTAACA 
GACACGTGTG TCTCCACTAA TGACGACGTC CCAGCGAGGA GAACAAGATC TCGCTCCAAC 
TCCTCCAACT CGGTCAGCTC AGAGAGGTCC GCTTTGAGCA TTAACCTGCA GGAGGGCGGA 
CGGAGAGGAG CAAGGAGGAC CAGTGAGCCG CCCCGCGTGG TTGCTTCCAG AAGCAGCACC 
AGGAGGAAGA CGGTGGCTGC ACGTCCAACA CAAGGGGACC GTAACGATGA TACTGGCATA 
ACGGGGGCCC AGGGCCAAGC ACGAGGAAGC AGGCAGCGCA CGAGAGGAGG AAAAGCTGAG 
GCAGTTTCTC CTGCGGTCAG TCAGAGTGAC CCCAAAACTG TAACCAGAGG CAGGAAGAAC 
ACTAAAGCCC AGTCCTCCAA CGATGCCGAG AAGGCAGGTT CTCAGCAGGC TACTACCTCT 
AGGGGGCAGC GTCGATCTAG TTCAAATGGC TCTGGACCCA CAGAAGCTAA AGGCTCCTCG 
AATCAAGGCG AGCTCATTGG CAATGAGGAA ACGCTTCAGT CTAAAAAGAA TGTAAGGGGC 
AGAAGCCATA AACCAGCAAA AAGGGAGGCA GTGGTGGCCC CACAATCCCC TGCTGTCAGT 
AGTCCAGGTG AGACAAAAGA GCTTAGAAAA GGAAGAAAAA GAGGGTCTGA AGCAAATACT 
GACATTGACT CGGACAGAAG TTCCAAATTC ATGAAAGGGG GGGAAATAGA ACAGACACCA 
GGAGAAGCTG AAGAAGCACC TAAACAAGCA CAAGATGAGA CTCCTGCACC AGCTAAGAGA 
AGAAGTAGAG CGTCCGTCGC TCAGACGAAG AAAGCTCTCG ATCCGTCTGG ACCTGGGCTG 
AAGGAAACTA ATGAAAGAAT AGCCGAGGAG CCCGTGGAGA AGAGAGGCCG AGGTCGACTG 
GCAGTGGGCC AGAAACAGAA AAGAGAGGAG CAGGAAGGTG CCGAAGTGCC TGTCGAGCAG 
CGTGCACGTG TGCTGGAAGC AGAGGTCCAA ACCCCAGCCA GCAGTGGGTC CAGGAAGCGC 
CAGGCTCCTG TGGATGCTTC GCCCTTCAGG AAGACCCCTC GCGCCTCCTC CCTGTCCCCA 
GCAGCTCACG TTCGCTCGCG TGCCGTCGGC CACGCGTACA AGGTGCTGTT CACAGGTGTG 
GTGGACGAAG CAGGGGAGCA AGTGTTGGCC CGTTTGGGAG GCAGCGTGGC CAAAGGCGTG 
GCCGACATGA ACTGTCTGGT GACCGATAAG CTGCGCCGGA CCGTGAAGCT CCTGTGTGCC 
GTGGCCAAAG GAATCCCTGT TGTTACCACG CTGTGGCTGG AAAAGAGTGG GAAGACTGGG 
AGCTTCCTGT CTCCCAGTTC CTTCATTGTG AAGGATCTGG AGCAGGAGAA GAAATTCAAT 
TTCTGCTTGC AGGAGTCTCT CAAGGTTGCC AGCAGCCAGC CGCTCTTACA GGGGTACGAA 
ATTCATGTTA CCAAGTCGGT GAAGCCTGAG CCAGTTCAGA TGAGAGACAT ACTCACATGC 
AGCGGCGCTA CCTTCCTCCC CAAGATGCCC AGTTCTCACA AGGCTCAAAC TGTAGTGATT 
TCATGTGAAG AGGACTGGCA CTTATGTGCT CGCGCCGCGT CGGCGTCAGT CCCCGTGGTC 
ACCGCCGAGT TCATCCTCGC CGGGATCCTG CAGCAGAAAC TGGACTTTCA GAGCCACGCG 
CTCTCCCCCC CTGCAGGCCG CCTGCAGCCT GTAGGCAGAG CGAGGCAACG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604224.2
CDS	128..6004
Translation	MDATQMINDSVLESDEEENEANQPSKGRPVAKLLILKNDHMPETELPLFLGENVLGRDGNTCTVPLPSPSISKQHATVSISLYRSRGESDLEALVWDLGSLNGTRKGRLKLTPNVRYALVAQDSLVLADIPCQFVSCSADTVSAQHNFQTPVSRSSLADASNEKGSGTDVGGEKSERVSDAKVSSPVLKDTKNTPMRTICLSFEQTPTQPEGTLVPESESDEEREGRANRRQKTLDSDSDLNKSSPTCSNFLTPTNKTVPESEDESPITPPFSTKNRPHRHVGFNKGETNINMGRQQLKEKPASEKEEEHFSMDSGPRMQENQETNAQLKYDNLPVAAPSVTTDAFQMDSDTDLEGEEDGVATLTTNQIVDGTSDKAQFCMESDTDVEEDAHASRKVPGPASLPENNTKPLPETPVFRPVDSDTDVDDDDTLGAASKAKPTSVQSRTTADSTPTTHLKQFHSDSDAESEEDDMKRVPSNSSFKISETPAKLAKGVSAVPPCPGSETHDDALPAIRKSGAAESWNAADTRADLDILSDSDTDAEHDSPLVKQTFVGTNMSLTRCPVSEAIQSDSDADTDVEESTTAPVLGRVTTASLQEGGEKDVEVEVNVAAPGEGHVPRLVREETPGLLNPSYQHCSTPVQLPENQVEDMETQAFLSPTSVRYTGAPALRTQALDLDSDSQEEENIVVAETQSFVLQTRACRGSRSEGHIREATQTSALESADDEMSKQSCREESFQLGLSESSHIQDPAAAADMESTQAFVSVEATQLYAATSSTSASELHAAPADGDQGEEPATGSAVPEREGWENLALEATQAYLSDPYHDSEEETDDEETQPFHGPASATLAMAETQPMCAFEEEDIKHPVSTAVQVKVRAKNETEERAEHSEAAVAQGRPLKVDLSLAETQPMCTSDDRESDDEDSFPGLRKGKTKQLQIEDEQTQPLTSSEQSAVETQPLQTVDQDLAAGGSEAFHQDNPVAAPRREVLQFKEEKPAALISSDLSTAETQLMTTGDDEESDEADAVLALPRRKAKPLQHQEDETQPAVCEPSVFQSRPLISGENHDGVGSLPGTEMTKVKPSQILEERIQLLTNSTSSTHETGVLETKADAEAGTSGSKVNGRRGTRANLRKVSTQCDEPSRRPTRGIKDSSAAARRGTSKSGEEKDEELEDKEEEHNSEAEGIQSRNDVDVLNKTEQNISQIQKQQEPSLGEQRGGTEPRQHENMEKERKETEENKRTETEPSNRLEWERAEKEQTVSTKKKDREDKERLEHDKAEREEKERLEKEKNVLMANRQTEQEKEMLESERRKRDENLGREDQQRSARQDSEAKGADRIEASEKEQLTEEKTVEKQEQKEENKAKVSARGRRAAQRTAVCKTEPVTDTCVSTNDDVPARRTRSRSNSSNSVSSERSALSINLQEGGRRGARRTSEPPRVVASRSSTRRKTVAARPTQGDRNDDTGITGAQGQARGSRQRTRGGKAEAVSPAVSQSDPKTVTRGRKNTKAQSSNDAEKAGSQQATTSRGQRRSSSNGSGPTEAKGSSNQGELIGNEETLQSKKNVRGRSHKPAKREAVVAPQSPAVSSPGETKELRKGRKRGSEANTDIDSDRSSKFMKGGEIEQTPGEAEEAPKQAQDETPAPAKRRSRASVAQTKKALDPSGPGLKETNERIAEEPVEKRGRGRLAVGQKQKREEQEGAEVPVEQRARVLEAEVQTPASSGSRKRQAPVDASPFRKTPRASSLSPAAHVRSRAVGHAYKVLFTGVVDEAGEQVLARLGGSVAKGVADMNCLVTDKLRRTVKLLCAVAKGIPVVTTLWLEKSGKTGSFLSPSSFIVKDLEQEKKFNFCLQESLKVASSQPLLQGYEIHVTKSVKPEPVQMRDILTCSGATFLPKMPSSHKAQTVVISCEEDWHLCARAASASVPVVTAEFILAGILQQKLDFQSHALSPPAGRLQPVGRARQRT

Target ORF information:

RefSeq Version	XM_011605922.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mediator of DNA damage checkpoint protein 1 (LOC105416732), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605922.2

ORF Insert Sequence:

ATGGATGCAA CTCAGATGAT AAATGACTCT GTGCTGGAGT CAGATGAGGA AGAAAATGAA 
GCTAATCAAC CCAGCAAGGG TAGACCGGTC GCTAAACTAC TCATTTTAAA AAATGATCAC 
ATGCCTGAGA CAGAATTACC ACTCTTTTTG GGAGAAAATG TTTTGGGCCG GGATGGTAAC 
ACTTGCACTG TGCCCTTGCC ATCTCCCTCC ATTTCCAAGC AGCATGCAAC CGTCTCAATC 
TCCTTGTATC GATCTAGAGG AGAATCAGAT CTGGAGGCTT TGGTTTGGGA TCTCGGGAGC 
CTGAATGGGA CCCGCAAAGG TCGCCTTAAG TTGACTCCAA ATGTACGCTA TGCCCTCGTT 
GCCCAGGACA GTTTGGTTTT GGCAGATATC CCGTGTCAGT TTGTCAGTTG TTCTGCAGAC 
ACAGTCTCTG CTCAGCATAA CTTCCAGACA CCTGTGAGTC GAAGCTCCCT GGCAGATGCC 
TCCAATGAAA AGGGAAGTGG CACCGATGTT GGTGGTGAGA AATCTGAAAG AGTTTCAGAT 
GCAAAGGTCT CATCACCAGT TCTCAAAGAC ACAAAGAATA CCCCCATGCG GACCATTTGC 
CTGTCCTTTG AGCAAACTCC AACTCAGCCA GAGGGCACCC TGGTCCCAGA ATCTGAATCA 
GATGAAGAGA GAGAGGGTCG AGCCAATCGG AGGCAGAAGA CTTTAGATTC TGATTCCGAC 
CTCAATAAAT CTAGTCCTAC CTGTTCAAAC TTCCTGACTC CCACAAACAA AACTGTCCCT 
GAAAGTGAGG ATGAAAGTCC TATTACACCA CCCTTCTCCA CTAAAAATAG GCCTCACAGA 
CATGTCGGAT TCAACAAAGG AGAGACAAAT ATAAATATGG GCCGACAGCA GCTGAAGGAA 
AAACCGGCAT CCGAAAAAGA GGAAGAACAT TTTTCCATGG ATAGTGGCCC TCGTATGCAA 
GAGAATCAGG AAACCAATGC CCAGCTCAAA TACGATAACC TGCCTGTTGC TGCACCATCA 
GTCACCACAG ACGCATTTCA AATGGACAGT GACACTGATT TGGAGGGAGA GGAGGACGGG 
GTGGCGACCT TGACTACAAA CCAAATTGTT GATGGCACAT CAGACAAAGC CCAGTTTTGC 
ATGGAGAGTG ATACGGATGT TGAGGAGGAC GCTCACGCCT CGCGCAAAGT TCCCGGACCT 
GCGTCTCTGC CTGAAAACAA CACAAAACCT TTACCTGAAA CTCCAGTCTT TCGGCCTGTG 
GACAGTGATA CGGACGTGGA TGATGACGAC ACATTGGGCG CTGCGTCAAA AGCTAAACCC 
ACTTCAGTCC AGAGCAGAAC CACTGCTGAC TCCACTCCAA CAACACACCT GAAACAGTTC 
CATTCTGACA GTGACGCAGA GAGCGAGGAG GACGACATGA AACGAGTGCC AAGTAATTCA 
TCCTTTAAAA TAAGCGAGAC ACCCGCTAAA TTAGCCAAAG GTGTTTCTGC TGTGCCGCCC 
TGCCCAGGCA GTGAGACGCA TGATGACGCC CTTCCTGCCA TCAGGAAATC TGGTGCCGCG 
GAATCGTGGA ATGCTGCAGA CACACGTGCT GATTTGGATA TTCTGTCTGA CAGCGACACG 
GATGCGGAAC ATGATTCTCC TCTGGTAAAA CAAACATTTG TTGGCACAAA TATGTCACTC 
ACACGCTGTC CTGTGTCAGA AGCCATCCAG TCAGATTCTG ACGCAGACAC AGATGTGGAA 
GAATCCACCA CAGCCCCCGT TTTGGGGAGG GTAACTACAG CCAGCCTTCA GGAAGGAGGA 
GAAAAGGATG TGGAAGTTGA GGTCAATGTT GCGGCGCCTG GAGAAGGTCA CGTCCCGCGC 
CTCGTCAGGG AGGAGACTCC TGGACTCTTG AATCCTTCCT ACCAGCACTG CTCTACTCCT 
GTCCAGTTGC CAGAAAATCA AGTGGAGGAT ATGGAGACCC AGGCCTTCCT GAGCCCCACC 
TCAGTGAGAT ATACGGGAGC TCCTGCTCTG AGGACTCAAG CATTAGATTT GGACTCAGAC 
AGTCAGGAGG AGGAGAACAT CGTTGTGGCC GAGACACAGT CTTTTGTTCT GCAGACCCGA 
GCCTGTCGGG GCAGTCGCTC GGAGGGCCAC ATCAGAGAAG CCACGCAGAC ATCCGCCCTC 
GAGTCTGCTG ATGATGAAAT GAGCAAACAG TCCTGCAGAG AAGAGTCTTT TCAGCTGGGA 
TTGTCTGAGA GCAGCCACAT TCAGGATCCA GCTGCAGCTG CAGACATGGA AAGCACCCAG 
GCATTTGTCT CTGTAGAGGC CACCCAACTA TATGCAGCCA CCTCATCGAC GAGTGCTTCA 
GAACTCCATG CTGCACCAGC TGATGGCGAT CAGGGGGAGG AGCCAGCCAC AGGCTCAGCT 
GTACCTGAAA GGGAAGGTTG GGAAAATTTG GCCTTAGAAG CAACACAGGC TTACCTTTCA 
GACCCATATC ATGATTCAGA AGAGGAAACG GATGATGAGG AGACTCAGCC CTTTCATGGT 
CCAGCCTCTG CTACCCTCGC CATGGCTGAA ACTCAACCAA TGTGTGCTTT TGAGGAGGAG 
GACATAAAGC ATCCTGTTTC CACTGCAGTG CAAGTTAAGG TCAGAGCAAA GAATGAAACA 
GAAGAGAGGG CAGAGCACAG TGAGGCGGCC GTAGCTCAGG GGAGGCCCCT CAAGGTGGAT 
CTGTCTCTAG CCGAAACTCA GCCCATGTGC ACCAGCGATG ACAGGGAAAG TGATGATGAG 
GATTCGTTTC CAGGTCTACG AAAAGGGAAA ACAAAACAGC TGCAGATTGA AGATGAGCAG 
ACGCAGCCCC TCACAAGTTC AGAGCAATCC GCTGTTGAAA CGCAGCCGCT GCAGACTGTT 
GACCAGGACC TGGCTGCAGG GGGAAGCGAG GCCTTTCATC AAGACAATCC AGTTGCAGCT 
CCACGCAGAG AAGTGCTGCA ATTTAAAGAG GAAAAGCCAG CAGCTCTAAT TAGCTCTGAT 
CTGTCCACTG CTGAAACGCA GCTCATGACC ACAGGTGACG ATGAAGAAAG TGACGAAGCT 
GACGCGGTTT TAGCTCTTCC AAGAAGGAAA GCAAAACCTC TGCAGCACCA AGAAGATGAG 
ACACAGCCGG CTGTTTGTGA GCCTTCGGTT TTCCAAAGCC GGCCACTGAT TTCAGGAGAA 
AACCACGATG GCGTTGGTTC ACTTCCTGGT ACAGAAATGA CAAAAGTAAA GCCTTCACAA 
ATCTTAGAAG AGAGGATACA GCTGCTCACA AATTCGACGT CCTCCACACA CGAAACTGGC 
GTGTTGGAAA CCAAAGCTGA CGCTGAGGCT GGAACCAGTG GAAGCAAAGT TAATGGTAGA 
AGAGGAACGA GGGCAAATTT GAGAAAGGTG TCAACACAGT GCGATGAACC TTCCAGGAGA 
CCGACAAGAG GGATAAAAGA TTCTTCAGCT GCAGCCCGTA GAGGAACATC AAAGTCTGGC 
GAAGAAAAGG ATGAGGAGCT GGAGGATAAG GAAGAGGAGC ATAACAGCGA AGCTGAGGGA 
ATTCAATCCA GAAATGATGT GGACGTTTTG AACAAGACTG AACAGAACAT TAGTCAAATC 
CAGAAACAAC AAGAACCGAG TCTGGGAGAG CAGAGAGGTG GCACTGAGCC AAGACAACAC 
GAAAACATGG AGAAGGAAAG GAAGGAAACA GAAGAAAACA AAAGAACTGA GACTGAACCT 
TCAAACAGGC TTGAATGGGA GAGAGCAGAA AAAGAGCAAA CAGTTTCAAC AAAGAAAAAA 
GACAGGGAAG ACAAAGAGAG ATTAGAGCAC GACAAGGCAG AAAGGGAGGA GAAGGAGAGA 
TTGGAAAAAG AGAAGAATGT TCTGATGGCC AACAGACAAA CAGAACAAGA AAAAGAAATG 
TTGGAGAGTG AAAGGAGGAA ACGTGATGAG AACCTTGGGA GGGAAGATCA ACAACGCAGT 
GCAAGACAGG ACAGTGAAGC AAAGGGGGCA GACAGAATAG AAGCCTCTGA GAAAGAACAG 
CTAACGGAGG AAAAGACAGT AGAAAAACAA GAGCAGAAGG AAGAAAACAA GGCCAAAGTG 
TCTGCAAGAG GACGAAGAGC AGCCCAGAGA ACCGCCGTGT GTAAAACAGA GCCAGTAACA 
GACACGTGTG TCTCCACTAA TGACGACGTC CCAGCGAGGA GAACAAGATC TCGCTCCAAC 
TCCTCCAACT CGGTCAGCTC AGAGAGGTCC GCTTTGAGCA TTAACCTGCA GGAGGGCGGA 
CGGAGAGGAG CAAGGAGGAC CAGTGAGCCG CCCCGCGTGG TTGCTTCCAG AAGCAGCACC 
AGGAGGAAGA CGGTGGCTGC ACGTCCAACA CAAGGGGACC GTAACGATGA TACTGGCATA 
ACGGGGGCCC AGGGCCAAGC ACGAGGAAGC AGGCAGCGCA CGAGAGGAGG AAAAGCTGAG 
GCAGTTTCTC CTGCGGTCAG TCAGAGTGAC CCCAAAACTG TAACCAGAGG CAGGAAGAAC 
ACTAAAGCCC AGTCCTCCAA CGATGCCGAG AAGGCAGGTT CTCAGCAGGC TACTACCTCT 
AGGGGGCAGC GTCGATCTAG TTCAAATGGC TCTGGACCCA CAGAAGCTAA AGGCTCCTCG 
AATCAAGGCG AGCTCATTGG CAATGAGGAA ACGCTTCAGT CTAAAAAGAA TGTAAGGGGC 
AGAAGCCATA AACCAGCAAA AAGGGAGGCA GTGGTGGCCC CACAATCCCC TGCTGTCAGT 
AGTCCAGGTG AGACAAAAGA GCTTAGAAAA GGAAGAAAAA GAGGGTCTGA AGCAAATACT 
GACATTGACT CGGACAGAAG TTCCAAATTC ATGAAAGGGG GGGAAATAGA ACAGACACCA 
GGAGAAGCTG AAGAAGCACC TAAACAAGCA CAAGATGAGA CTCCTGCACC AGCTAAGAGA 
AGAAGTAGAG CGTCCGTCGC TCAGACGAAG AAAGCTCTCG ATCCGTCTGG ACCTGGGCTG 
AAGGAAACTA ATGAAAGAAT AGCCGAGGAG CCCGTGGAGA AGAGAGGCCG AGGTCGACTG 
GCAGTGGGCC AGAAACAGAA AAGAGAGGAG CAGGAAGGTG CCGAAGTGCC TGTCGAGCAG 
CGTGCACGTG TGCTGGAAGC AGAGGTCCAA ACCCCAGCCA GCAGTGGGTC CAGGAAGCGC 
CAGGCTCCTG TGGATGCTTC GCCCTTCAGG AAGACCCCTC GCGCCTCCTC CCTGTCCCCA 
GCAGCTCACG TTCGCTCGCG TGCCGTCGGC CACGCGTACA AGGTGCTGTT CACAGGTGTG 
GTGGACGAAG CAGGGGAGCA AGTGTTGGCC CGTTTGGGAG GCAGCGTGGC CAAAGGCGTG 
GCCGACATGA ACTGTCTGGT GACCGATAAG CTGCGCCGGA CCGTGAAGCT CCTGTGTGCC 
GTGGCCAAAG GAATCCCTGT TGTTACCACG CTGTGGCTGG AAAAGAGTGG GAAGACTGGG 
AGCTTCCTGT CTCCCAGTTC CTTCATTGTG AAGGATCTGG AGCAGGAGAA GAAATTCAAT 
TTCTGCTTGC AGGAGTCTCT CAAGGTTGCC AGCAGCCAGC CGCTCTTACA GGGGTACGAA 
ATTCATGTTA CCAAGTCGGT GAAGCCTGAG CCAGTTCAGA TGAGAGACAT ACTCACATGC 
AGCGGCGCTA CCTTCCTCCC CAAGATGCCC AGTTCTCACA AGGCTCAAAC TGTAGTGATT 
TCATGTGAAG AGGACTGGCA CTTATGTGCT CGCGCCGCGT CGGCGTCAGT CCCCGTGGTC 
ACCGCCGAGT TCATCCTCGC CGGGATCCTG CAGCAGAAAC TGGACTTTCA GAGCCACGCG 
CTCTCCCCCC CTGCAGGCCG CCTGCAGCCT GTAGGCAGAG CGAGGCAACG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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mdc1 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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