LOC101078974 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101078974
Entrez Gene ID	101078974
Full Name	VPS8, CORVET complex subunit
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011602839.1	XP_011601141.1	vacuolar protein sorting-associated protein 8 homolog isoform X1
XM_011602839.2	XP_011601141.1	vacuolar protein sorting-associated protein 8 homolog isoform X2
XM_011602840.1	XP_011601142.1	vacuolar protein sorting-associated protein 8 homolog isoform X2
XM_029835098.1	XP_029690958.1	vacuolar protein sorting-associated protein 8 homolog isoform X1
XM_029835099.1	XP_029690959.1	vacuolar protein sorting-associated protein 8 homolog isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC101078974 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101078974 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo31496	XM_011602839.1 Latest version!	Takifugu rubripes vacuolar protein sorting 8 homolog (S. cerevisiae) (vps8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo31497	XM_011602840.1 Latest version!	Takifugu rubripes vacuolar protein sorting 8 homolog (S. cerevisiae) (vps8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo65165	XM_029835098.1 Latest version!	Takifugu rubripes VPS8 subunit of CORVET complex (LOC101078974), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo65166	XM_011602839.2 Latest version!	Takifugu rubripes VPS8 subunit of CORVET complex (LOC101078974), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo65167	XM_029835099.1 Latest version!	Takifugu rubripes VPS8 subunit of CORVET complex (LOC101078974), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101078974 ( XM_011602839.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601141.1 Takifugu rubripes vacuolar protein sorting 8 homolog (S. cerevisiae) (vps8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo31496
Clone ID Related Accession (Same CDS sequence)	XM_011602839.1
Accession Version	XM_011602839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070938.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGACGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGATA CCAACCAGGC TCTGCGCCTC 
TGCCTGGGGA ACAAAGCAAC CGGGGCAGAT TACGGAGCTG TATCTGCCCT CAGCATCAAC 
CATGACTGCT CACGTCTGCT CTGTGGGTTT GCCAAGGGAC AGATTACAAT GTGGGATCTC 
GCCAATGGAA AACTCCTGAG AACTATTACT GATGCACACC CCCCAGGGAC GGCTATACTT 
CACGTGAAGT TTACAGACGA TCCAACTCTT GCAGTTTGCA ATGACAGTGG AGGATCCGTG 
TTTGAGCTGG CGTTCAGGAG AGTGATGGGG ATGCGCTCCT GTGACTCTCG ATGTCTCTTC 
AGCGGTTCCA AAGGGGAGGT GTGCTCTGTT GAACCCCTGC ATGCTCCTCC AGAGGCCAGA 
GATCACCCCA TCACCCACTA TTCTCTCCTG GCCATGGCCT CTCTCACTAA GATTCTGGTT 
ATTGGCCTGA AGCCCTCACT TAAAGTCTGG ATAACGTTCC CCTACAGTAA ATCTGATCCG 
TCCAGTGTTC CCCAGCTGGC CTGGCAGTTT GTTCCAGTCC AGAGAACCGT GAACCCCGTC 
CTGGCCTTTT CCAAAGGAGA TACAGTTCAC TTCCTCTTGG TAAAAAAGGA GGAAACAGGC 
ACCATCCATG TCATCAAACA AAGGCAGCTC CATGTCAGCT GTGACATCAT CAGCCTCTGT 
TGGATCAACA GCCGCACGCT GGTCTTGGTG GACAGCCATG AAAAACTGCA GGTTGTGGAC 
CGACCCAGTC AGGAGGTTTT GGAGACCCTC GACTTGGAAC AGGTGCAGCT GGTTTATAAC 
AGCAGACATT TCAAATCGCT GGCCACTGGA GGGAATGTCT CCCAGGCTCT GGCTCTCGTT 
GGAGAAAAGG CCTGTTACCA GTCGGTCTCC AGCTACGCGG GACAGATTAT ATATCTGGGC 
ACCAAGTCTG CTCACATCAT GACTCTCAGG GCCTGGAGGG AGCGGATCGA CTGTCTGCTG 
AAGCTGGAAC ATTTTCCCGA GGCCCTGTCT CTGGCCTGGT CGTTCCACGA GGGGACAGCT 
AAGGCTGTGG TCGGTCTGTT TGGAGACCCG GTGAAAAGAA AAGGAGTCGT TGCTGACAAG 
ATGGTAGAGA TGATTTTCCA GTTTGCAGAC TTTGCCCTAA AGAAGTGTCC AGAACAAGGA 
AAGATCCAAG TGCTGGAACA ACACTTCCAT GAAGTGGTTC CAGTCCTGGT TGATTACTGT 
TTGCTGCTGA AGAGGCTTGA CCTGCTGTTT AACCAGCTGT ACCCTCAACT GGTGGAGAAC 
ATGGTGGCTA AAGGCATCTT CTTGGAATCC CTGGAGTCCT ACATTATCGC AGACCGCCTT 
GGTCACCTTC CCACACCGGT CATGAAAGAC CTTCTCATGC ATTACCAAAG CAGCTGTATG 
ATGGAGAGCC TGGAGAGATG CATCGTCCAT CTGGACATCA CCAGCCTGGA CATACAGCAG 
GTGGTTCAGG TGTGTTGGGA GAACCAACTC TACGATGCGG TGATCTACGT TTTCAATCGT 
GGCATGAACG ACTACACTAC ACCTATGGAA AAACTCTTTG CAGTAATTGT CCCACCAGTA 
AGGGAGGGGA AGAGTCTTAC AGATGTGGAA GTGGTGATGG GAAACAAACT CCTGGTGTAT 
ATCAGCTGCT GTCTGGCAGG ACGGGCATAT CCATTAGGAG ACATCCCCGA AGACCTTGTG 
GTCCAGGTAA AGCATCAGGT GTTTGAATTT TTGATCCGTC GCCATTCAGC GGACTCCTCA 
GAGGAGGAAG AAGTGTTCCC GTTTATTCGC ACTCTGCTCC ACTTTGACAC TCGAGAGTTC 
CTCAACGTCC TCGCCATGAC ATTCGAAGAC TTCAAGAACG ACAAACAGGC CCTTGAATAC 
CAGCAGAGGA TCGTGGACAT CTTACTGCAG GTGATGGTGG ACAACCCAGA CTTTACTCCG 
TCCCAGGTGG GAGGACTCTT CACCTTTTTA GCGCGACAGT TGGCCAAACC AGACAACACA 
CTTTTCGTAA ACAGGAAGCT CTTCGATCAG GTCCTGGAGT TTCTGTGCTG CCCAGATGAC 
GACTCCCGTC ACACTGAGAG GCAGCAGGTT CTGTTGGAGC TGCTGCAGGT TGGGAGCGTG 
GTCCAGTTTA ATGAAGAGAG GCTTCTGATG CTGGCCGAGA AAGCCAAATT CTACCAGATA 
TGTGAATTTC TTTATGAGAA GCAACATCTC TACGACAGGA TCATTGACTG TTACCTGAGA 
GACCCCCTCA GAAAGGGAGA GGTCTTCAGC TACATCCACA ATCTGCTGTC CATGCCTGGT 
TACAGCCCGG AGGAGAAGCA GTCAGTCATG GATAAAGTTC GGCAGCATAT GCATGAGCTG 
GTGATCTTTG ACCCCAATAA ATCAGCTGAT TTAGTGACGT TTCACTTTGC TGAAGAGGTC 
CAGCAAATAA TCTCTGAGCT TCAGGATGAT CGGCTGCTCT TCAGGTTCCT CAGCTGTCTC 
TTGGAGCAAC AGGACGGCCA ACATTCGGGC CCCGTCCTAC TGCTGCAACC TGAACTCCAT 
GAGCTTCAGC TGGACCTGCT CAGCCGTTTT GACCCCAAAC ACATTTTGAA TTTCCTCCAG 
ACCTCCCAAC ACTATAGACT AGAAGAGGCA GTACAGATAA CACAGAAGTA TCACCACAAC 
AAAGCTACAG CCTTTCTTTT GGAGAAGAGG GGCGATGTCG AAGGAGCTTT TGCTGTGCTG 
TTGGAGACGC TAAAGGAAAA CCTGAGTCGT GTCACAGCTG AGAGAGCAGG AGATGATGAA 
GAGGAAACCC TGACAAATGT CAAGGATTCT CTAAGTGACA TCATCTCCTT GTGTCACCAG 
AGCTCTCAGA GCCTTAACCA GCAGCAAAGA GAGGTCCTGT GGTTTCCACT GTTGGAGACC 
ATGATGGCTG CACAAAAACA AGTGAAGGGA CGGGACAGTA AACACACATT TGAGGTGCTG 
AAGGAGCTGA CGATGAAGGT CCTCAACCGT ATGAGCAGCT TCATTTCTCT TCCCGCCATC 
ATCCAGCGGA TACTCCAGGA TCCAGTCTAC GGGAAAGGAA AGCTGGCAGA AATCCAGAGC 
CTTATTTTGG GCATGCTCGA CACTTTCAAC TATGAGCAGA CGTTACTTGA AACAACCACC 
AGTTTGTTGA ACCATGACCT CCACTGGTCC CTGGCGCACT TGCGCGCAGC GGTCACTAGG 
GGGATCCACC CACGACAGGA CTGCTGCAAC ATCTGCCTGC AGCAGTACAA GAGGAGGCAG 
GATGCAGCAG CAGCAGACGA GATCATCGTC TTCAGCTGTG GGCATCTGTA CCACCTCCAG 
TGTCTGCAGC AGAAGGACTC CGGCTGGGGT TTTACGTCAG ATGTTCAGCA GCAATGGAGC 
TGCTACAAAT GTTCCTCCAG CAAAGGAGGC CGAGCGGCGT CGGCTGATTC GAGAGGCCGC 
AGCTTGTCCC TGGCGCAGAC CTGCGTTACA TCATTACAAC ACGATGGGGC AACAGAAGCT 
GACGGGAAGA AGGGGTTTGT CGAAGTCACC TTAGACCATC AGCAAGAACA GTCCTGGGAT 
CAGCTACGCT GCCTTTACAG AGGACCGTCC AGGCTGTCCA TCCTGTCCGA ACTTTCACAC 
AGTAACGAGA AGACGGGCCT TCTGATCGGC AGCCAGACGG GGACAGATCA CTTCCAGCTG 
AAACTGGCCC CCCCGCCTCT GTTCGAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601141.1
CDS	31..4203
Translation	MSDSPDARSLLSASSQLNLMEVDAIDDKEFDIPQVDTPPTLESILNELEDEEEPFVLEDTCVLNTDNMDAHSCDTSSLASSDSGDPVHHKRRRRTVDMKPTLHGSVLRHSLLKGISAQIISAADKVDAGLPTAITMSGVIVVGTSHGLALVFDTNQALRLCLGNKATGADYGAVSALSINHDCSRLLCGFAKGQITMWDLANGKLLRTITDAHPPGTAILHVKFTDDPTLAVCNDSGGSVFELAFRRVMGMRSCDSRCLFSGSKGEVCSVEPLHAPPEARDHPITHYSLLAMASLTKILVIGLKPSLKVWITFPYSKSDPSSVPQLAWQFVPVQRTVNPVLAFSKGDTVHFLLVKKEETGTIHVIKQRQLHVSCDIISLCWINSRTLVLVDSHEKLQVVDRPSQEVLETLDLEQVQLVYNSRHFKSLATGGNVSQALALVGEKACYQSVSSYAGQIIYLGTKSAHIMTLRAWRERIDCLLKLEHFPEALSLAWSFHEGTAKAVVGLFGDPVKRKGVVADKMVEMIFQFADFALKKCPEQGKIQVLEQHFHEVVPVLVDYCLLLKRLDLLFNQLYPQLVENMVAKGIFLESLESYIIADRLGHLPTPVMKDLLMHYQSSCMMESLERCIVHLDITSLDIQQVVQVCWENQLYDAVIYVFNRGMNDYTTPMEKLFAVIVPPVREGKSLTDVEVVMGNKLLVYISCCLAGRAYPLGDIPEDLVVQVKHQVFEFLIRRHSADSSEEEEVFPFIRTLLHFDTREFLNVLAMTFEDFKNDKQALEYQQRIVDILLQVMVDNPDFTPSQVGGLFTFLARQLAKPDNTLFVNRKLFDQVLEFLCCPDDDSRHTERQQVLLELLQVGSVVQFNEERLLMLAEKAKFYQICEFLYEKQHLYDRIIDCYLRDPLRKGEVFSYIHNLLSMPGYSPEEKQSVMDKVRQHMHELVIFDPNKSADLVTFHFAEEVQQIISELQDDRLLFRFLSCLLEQQDGQHSGPVLLLQPELHELQLDLLSRFDPKHILNFLQTSQHYRLEEAVQITQKYHHNKATAFLLEKRGDVEGAFAVLLETLKENLSRVTAERAGDDEEETLTNVKDSLSDIISLCHQSSQSLNQQQREVLWFPLLETMMAAQKQVKGRDSKHTFEVLKELTMKVLNRMSSFISLPAIIQRILQDPVYGKGKLAEIQSLILGMLDTFNYEQTLLETTTSLLNHDLHWSLAHLRAAVTRGIHPRQDCCNICLQQYKRRQDAAAADEIIVFSCGHLYHLQCLQQKDSGWGFTSDVQQQWSCYKCSSSKGGRAASADSRGRSLSLAQTCVTSLQHDGATEADGKKGFVEVTLDHQQEQSWDQLRCLYRGPSRLSILSELSHSNEKTGLLIGSQTGTDHFQLKLAPPPLFEE

Target ORF information:

RefSeq Version	XM_011602839.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes vacuolar protein sorting 8 homolog (S. cerevisiae) (vps8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602839.1

ORF Insert Sequence:

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGACGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGATA CCAACCAGGC TCTGCGCCTC 
TGCCTGGGGA ACAAAGCAAC CGGGGCAGAT TACGGAGCTG TATCTGCCCT CAGCATCAAC 
CATGACTGCT CACGTCTGCT CTGTGGGTTT GCCAAGGGAC AGATTACAAT GTGGGATCTC 
GCCAATGGAA AACTCCTGAG AACTATTACT GATGCACACC CCCCAGGGAC GGCTATACTT 
CACGTGAAGT TTACAGACGA TCCAACTCTT GCAGTTTGCA ATGACAGTGG AGGATCCGTG 
TTTGAGCTGG CGTTCAGGAG AGTGATGGGG ATGCGCTCCT GTGACTCTCG ATGTCTCTTC 
AGCGGTTCCA AAGGGGAGGT GTGCTCTGTT GAACCCCTGC ATGCTCCTCC AGAGGCCAGA 
GATCACCCCA TCACCCACTA TTCTCTCCTG GCCATGGCCT CTCTCACTAA GATTCTGGTT 
ATTGGCCTGA AGCCCTCACT TAAAGTCTGG ATAACGTTCC CCTACAGTAA ATCTGATCCG 
TCCAGTGTTC CCCAGCTGGC CTGGCAGTTT GTTCCAGTCC AGAGAACCGT GAACCCCGTC 
CTGGCCTTTT CCAAAGGAGA TACAGTTCAC TTCCTCTTGG TAAAAAAGGA GGAAACAGGC 
ACCATCCATG TCATCAAACA AAGGCAGCTC CATGTCAGCT GTGACATCAT CAGCCTCTGT 
TGGATCAACA GCCGCACGCT GGTCTTGGTG GACAGCCATG AAAAACTGCA GGTTGTGGAC 
CGACCCAGTC AGGAGGTTTT GGAGACCCTC GACTTGGAAC AGGTGCAGCT GGTTTATAAC 
AGCAGACATT TCAAATCGCT GGCCACTGGA GGGAATGTCT CCCAGGCTCT GGCTCTCGTT 
GGAGAAAAGG CCTGTTACCA GTCGGTCTCC AGCTACGCGG GACAGATTAT ATATCTGGGC 
ACCAAGTCTG CTCACATCAT GACTCTCAGG GCCTGGAGGG AGCGGATCGA CTGTCTGCTG 
AAGCTGGAAC ATTTTCCCGA GGCCCTGTCT CTGGCCTGGT CGTTCCACGA GGGGACAGCT 
AAGGCTGTGG TCGGTCTGTT TGGAGACCCG GTGAAAAGAA AAGGAGTCGT TGCTGACAAG 
ATGGTAGAGA TGATTTTCCA GTTTGCAGAC TTTGCCCTAA AGAAGTGTCC AGAACAAGGA 
AAGATCCAAG TGCTGGAACA ACACTTCCAT GAAGTGGTTC CAGTCCTGGT TGATTACTGT 
TTGCTGCTGA AGAGGCTTGA CCTGCTGTTT AACCAGCTGT ACCCTCAACT GGTGGAGAAC 
ATGGTGGCTA AAGGCATCTT CTTGGAATCC CTGGAGTCCT ACATTATCGC AGACCGCCTT 
GGTCACCTTC CCACACCGGT CATGAAAGAC CTTCTCATGC ATTACCAAAG CAGCTGTATG 
ATGGAGAGCC TGGAGAGATG CATCGTCCAT CTGGACATCA CCAGCCTGGA CATACAGCAG 
GTGGTTCAGG TGTGTTGGGA GAACCAACTC TACGATGCGG TGATCTACGT TTTCAATCGT 
GGCATGAACG ACTACACTAC ACCTATGGAA AAACTCTTTG CAGTAATTGT CCCACCAGTA 
AGGGAGGGGA AGAGTCTTAC AGATGTGGAA GTGGTGATGG GAAACAAACT CCTGGTGTAT 
ATCAGCTGCT GTCTGGCAGG ACGGGCATAT CCATTAGGAG ACATCCCCGA AGACCTTGTG 
GTCCAGGTAA AGCATCAGGT GTTTGAATTT TTGATCCGTC GCCATTCAGC GGACTCCTCA 
GAGGAGGAAG AAGTGTTCCC GTTTATTCGC ACTCTGCTCC ACTTTGACAC TCGAGAGTTC 
CTCAACGTCC TCGCCATGAC ATTCGAAGAC TTCAAGAACG ACAAACAGGC CCTTGAATAC 
CAGCAGAGGA TCGTGGACAT CTTACTGCAG GTGATGGTGG ACAACCCAGA CTTTACTCCG 
TCCCAGGTGG GAGGACTCTT CACCTTTTTA GCGCGACAGT TGGCCAAACC AGACAACACA 
CTTTTCGTAA ACAGGAAGCT CTTCGATCAG GTCCTGGAGT TTCTGTGCTG CCCAGATGAC 
GACTCCCGTC ACACTGAGAG GCAGCAGGTT CTGTTGGAGC TGCTGCAGGT TGGGAGCGTG 
GTCCAGTTTA ATGAAGAGAG GCTTCTGATG CTGGCCGAGA AAGCCAAATT CTACCAGATA 
TGTGAATTTC TTTATGAGAA GCAACATCTC TACGACAGGA TCATTGACTG TTACCTGAGA 
GACCCCCTCA GAAAGGGAGA GGTCTTCAGC TACATCCACA ATCTGCTGTC CATGCCTGGT 
TACAGCCCGG AGGAGAAGCA GTCAGTCATG GATAAAGTTC GGCAGCATAT GCATGAGCTG 
GTGATCTTTG ACCCCAATAA ATCAGCTGAT TTAGTGACGT TTCACTTTGC TGAAGAGGTC 
CAGCAAATAA TCTCTGAGCT TCAGGATGAT CGGCTGCTCT TCAGGTTCCT CAGCTGTCTC 
TTGGAGCAAC AGGACGGCCA ACATTCGGGC CCCGTCCTAC TGCTGCAACC TGAACTCCAT 
GAGCTTCAGC TGGACCTGCT CAGCCGTTTT GACCCCAAAC ACATTTTGAA TTTCCTCCAG 
ACCTCCCAAC ACTATAGACT AGAAGAGGCA GTACAGATAA CACAGAAGTA TCACCACAAC 
AAAGCTACAG CCTTTCTTTT GGAGAAGAGG GGCGATGTCG AAGGAGCTTT TGCTGTGCTG 
TTGGAGACGC TAAAGGAAAA CCTGAGTCGT GTCACAGCTG AGAGAGCAGG AGATGATGAA 
GAGGAAACCC TGACAAATGT CAAGGATTCT CTAAGTGACA TCATCTCCTT GTGTCACCAG 
AGCTCTCAGA GCCTTAACCA GCAGCAAAGA GAGGTCCTGT GGTTTCCACT GTTGGAGACC 
ATGATGGCTG CACAAAAACA AGTGAAGGGA CGGGACAGTA AACACACATT TGAGGTGCTG 
AAGGAGCTGA CGATGAAGGT CCTCAACCGT ATGAGCAGCT TCATTTCTCT TCCCGCCATC 
ATCCAGCGGA TACTCCAGGA TCCAGTCTAC GGGAAAGGAA AGCTGGCAGA AATCCAGAGC 
CTTATTTTGG GCATGCTCGA CACTTTCAAC TATGAGCAGA CGTTACTTGA AACAACCACC 
AGTTTGTTGA ACCATGACCT CCACTGGTCC CTGGCGCACT TGCGCGCAGC GGTCACTAGG 
GGGATCCACC CACGACAGGA CTGCTGCAAC ATCTGCCTGC AGCAGTACAA GAGGAGGCAG 
GATGCAGCAG CAGCAGACGA GATCATCGTC TTCAGCTGTG GGCATCTGTA CCACCTCCAG 
TGTCTGCAGC AGAAGGACTC CGGCTGGGGT TTTACGTCAG ATGTTCAGCA GCAATGGAGC 
TGCTACAAAT GTTCCTCCAG CAAAGGAGGC CGAGCGGCGT CGGCTGATTC GAGAGGCCGC 
AGCTTGTCCC TGGCGCAGAC CTGCGTTACA TCATTACAAC ACGATGGGGC AACAGAAGCT 
GACGGGAAGA AGGGGTTTGT CGAAGTCACC TTAGACCATC AGCAAGAACA GTCCTGGGAT 
CAGCTACGCT GCCTTTACAG AGGACCGTCC AGGCTGTCCA TCCTGTCCGA ACTTTCACAC 
AGTAACGAGA AGACGGGCCT TCTGATCGGC AGCCAGACGG GGACAGATCA CTTCCAGCTG 
AAACTGGCCC CCCCGCCTCT GTTCGAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078974 ( XM_011602840.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601142.1 Takifugu rubripes vacuolar protein sorting 8 homolog (S. cerevisiae) (vps8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo31497
Clone ID Related Accession (Same CDS sequence)	XM_011602840.1
Accession Version	XM_011602840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070938.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGACGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGATA CCAACCAGGC TCTGCGCCTC 
TGCCTGGGGA ACAAAGCAAC CGGGGCAGAT TACGGAGCTG TATCTGCCCT CAGCATCAAC 
CATGACTGCT CACGTCTGCT CTGTGGGTTT GCCAAGGGAC AGATTACAAT GTGGGATCTC 
GCCAATGGAA AACTCCTGAG AACTATTACT GATGCACACC CCCCAGGGAC GGCTATACTT 
CACGTGAAGT TTACAGACGA TCCAACTCTT GCAGTTTGCA ATGACAGTGG AGGATCCGTG 
TTTGAGCTGG CGTTCAGGAG AGTGATGGGG ATGCGCTCCT GTGACTCTCG ATGTCTCTTC 
AGCGGTTCCA AAGGGGAGGT GTGCTCTGTT GAACCCCTGC ATGCTCCTCC AGAGGCCAGA 
GATCACCCCA TCACCCACTA TTCTCTCCTG GCCATGGCCT CTCTCACTAA GATTCTGGTT 
ATTGGCCTGA AGCCCTCACT TAAAGTCTGG ATAACGTTCC CCTACAGTAA ATCTGATCCG 
TCCAGTGTTC CCCAGCTGGC CTGGCAGTTT GTTCCAGTCC AGAGAACCGT GAACCCCGTC 
CTGGCCTTTT CCAAAGGAGA TACAGTTCAC TTCCTCTTGG TAAAAAAGGA GGAAACAGGC 
ACCATCCATG TCATCAAACA AAGGCAGCTC CATGTCAGCT GTGACATCAT CAGCCTCTGT 
TGGATCAACA GCCGCACGCT GGTCTTGGTG GACAGCCATG AAAAACTGCA GGTTGTGGAC 
CGACCCAGTC AGGAGGTTTT GGAGACCCTC GACTTGGAAC AGGCTCTCGT TGGAGAAAAG 
GCCTGTTACC AGTCGGTCTC CAGCTACGCG GGACAGATTA TATATCTGGG CACCAAGTCT 
GCTCACATCA TGACTCTCAG GGCCTGGAGG GAGCGGATCG ACTGTCTGCT GAAGCTGGAA 
CATTTTCCCG AGGCCCTGTC TCTGGCCTGG TCGTTCCACG AGGGGACAGC TAAGGCTGTG 
GTCGGTCTGT TTGGAGACCC GGTGAAAAGA AAAGGAGTCG TTGCTGACAA GATGGTAGAG 
ATGATTTTCC AGTTTGCAGA CTTTGCCCTA AAGAAGTGTC CAGAACAAGG AAAGATCCAA 
GTGCTGGAAC AACACTTCCA TGAAGTGGTT CCAGTCCTGG TTGATTACTG TTTGCTGCTG 
AAGAGGCTTG ACCTGCTGTT TAACCAGCTG TACCCTCAAC TGGTGGAGAA CATGGTGGCT 
AAAGGCATCT TCTTGGAATC CCTGGAGTCC TACATTATCG CAGACCGCCT TGGTCACCTT 
CCCACACCGG TCATGAAAGA CCTTCTCATG CATTACCAAA GCAGCTGTAT GATGGAGAGC 
CTGGAGAGAT GCATCGTCCA TCTGGACATC ACCAGCCTGG ACATACAGCA GGTGGTTCAG 
GTGTGTTGGG AGAACCAACT CTACGATGCG GTGATCTACG TTTTCAATCG TGGCATGAAC 
GACTACACTA CACCTATGGA AAAACTCTTT GCAGTAATTG TCCCACCAGT AAGGGAGGGG 
AAGAGTCTTA CAGATGTGGA AGTGGTGATG GGAAACAAAC TCCTGGTGTA TATCAGCTGC 
TGTCTGGCAG GACGGGCATA TCCATTAGGA GACATCCCCG AAGACCTTGT GGTCCAGGTA 
AAGCATCAGG TGTTTGAATT TTTGATCCGT CGCCATTCAG CGGACTCCTC AGAGGAGGAA 
GAAGTGTTCC CGTTTATTCG CACTCTGCTC CACTTTGACA CTCGAGAGTT CCTCAACGTC 
CTCGCCATGA CATTCGAAGA CTTCAAGAAC GACAAACAGG CCCTTGAATA CCAGCAGAGG 
ATCGTGGACA TCTTACTGCA GGTGATGGTG GACAACCCAG ACTTTACTCC GTCCCAGGTG 
GGAGGACTCT TCACCTTTTT AGCGCGACAG TTGGCCAAAC CAGACAACAC ACTTTTCGTA 
AACAGGAAGC TCTTCGATCA GGTCCTGGAG TTTCTGTGCT GCCCAGATGA CGACTCCCGT 
CACACTGAGA GGCAGCAGGT TCTGTTGGAG CTGCTGCAGG TTGGGAGCGT GGTCCAGTTT 
AATGAAGAGA GGCTTCTGAT GCTGGCCGAG AAAGCCAAAT TCTACCAGAT ATGTGAATTT 
CTTTATGAGA AGCAACATCT CTACGACAGG ATCATTGACT GTTACCTGAG AGACCCCCTC 
AGAAAGGGAG AGGTCTTCAG CTACATCCAC AATCTGCTGT CCATGCCTGG TTACAGCCCG 
GAGGAGAAGC AGTCAGTCAT GGATAAAGTT CGGCAGCATA TGCATGAGCT GGTGATCTTT 
GACCCCAATA AATCAGCTGA TTTAGTGACG TTTCACTTTG CTGAAGAGGT CCAGCAAATA 
ATCTCTGAGC TTCAGGATGA TCGGCTGCTC TTCAGGTTCC TCAGCTGTCT CTTGGAGCAA 
CAGGACGGCC AACATTCGGG CCCCGTCCTA CTGCTGCAAC CTGAACTCCA TGAGCTTCAG 
CTGGACCTGC TCAGCCGTTT TGACCCCAAA CACATTTTGA ATTTCCTCCA GACCTCCCAA 
CACTATAGAC TAGAAGAGGC AGTACAGATA ACACAGAAGT ATCACCACAA CAAAGCTACA 
GCCTTTCTTT TGGAGAAGAG GGGCGATGTC GAAGGAGCTT TTGCTGTGCT GTTGGAGACG 
CTAAAGGAAA ACCTGAGTCG TGTCACAGCT GAGAGAGCAG GAGATGATGA AGAGGAAACC 
CTGACAAATG TCAAGGATTC TCTAAGTGAC ATCATCTCCT TGTGTCACCA GAGCTCTCAG 
AGCCTTAACC AGCAGCAAAG AGAGGTCCTG TGGTTTCCAC TGTTGGAGAC CATGATGGCT 
GCACAAAAAC AAGTGAAGGG ACGGGACAGT AAACACACAT TTGAGGTGCT GAAGGAGCTG 
ACGATGAAGG TCCTCAACCG TATGAGCAGC TTCATTTCTC TTCCCGCCAT CATCCAGCGG 
ATACTCCAGG ATCCAGTCTA CGGGAAAGGA AAGCTGGCAG AAATCCAGAG CCTTATTTTG 
GGCATGCTCG ACACTTTCAA CTATGAGCAG ACGTTACTTG AAACAACCAC CAGTTTGTTG 
AACCATGACC TCCACTGGTC CCTGGCGCAC TTGCGCGCAG CGGTCACTAG GGGGATCCAC 
CCACGACAGG ACTGCTGCAA CATCTGCCTG CAGCAGTACA AGAGGAGGCA GGATGCAGCA 
GCAGCAGACG AGATCATCGT CTTCAGCTGT GGGCATCTGT ACCACCTCCA GTGTCTGCAG 
CAGAAGGACT CCGGCTGGGG TTTTACGTCA GATGTTCAGC AGCAATGGAG CTGCTACAAA 
TGTTCCTCCA GCAAAGGAGG CCGAGCGGCG TCGGCTGATT CGAGAGGCCG CAGCTTGTCC 
CTGGCGCAGA CCTGCGTTAC ATCATTACAA CACGATGGGG CAACAGAAGC TGACGGGAAG 
AAGGGGTTTG TCGAAGTCAC CTTAGACCAT CAGCAAGAAC AGTCCTGGGA TCAGCTACGC 
TGCCTTTACA GAGGACCGTC CAGGCTGTCC ATCCTGTCCG AACTTTCACA CAGTAACGAG 
AAGACGGGCC TTCTGATCGG CAGCCAGACG GGGACAGATC ACTTCCAGCT GAAACTGGCC 
CCCCCGCCTC TGTTCGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601142.1
CDS	31..4134
Translation	MSDSPDARSLLSASSQLNLMEVDAIDDKEFDIPQVDTPPTLESILNELEDEEEPFVLEDTCVLNTDNMDAHSCDTSSLASSDSGDPVHHKRRRRTVDMKPTLHGSVLRHSLLKGISAQIISAADKVDAGLPTAITMSGVIVVGTSHGLALVFDTNQALRLCLGNKATGADYGAVSALSINHDCSRLLCGFAKGQITMWDLANGKLLRTITDAHPPGTAILHVKFTDDPTLAVCNDSGGSVFELAFRRVMGMRSCDSRCLFSGSKGEVCSVEPLHAPPEARDHPITHYSLLAMASLTKILVIGLKPSLKVWITFPYSKSDPSSVPQLAWQFVPVQRTVNPVLAFSKGDTVHFLLVKKEETGTIHVIKQRQLHVSCDIISLCWINSRTLVLVDSHEKLQVVDRPSQEVLETLDLEQALVGEKACYQSVSSYAGQIIYLGTKSAHIMTLRAWRERIDCLLKLEHFPEALSLAWSFHEGTAKAVVGLFGDPVKRKGVVADKMVEMIFQFADFALKKCPEQGKIQVLEQHFHEVVPVLVDYCLLLKRLDLLFNQLYPQLVENMVAKGIFLESLESYIIADRLGHLPTPVMKDLLMHYQSSCMMESLERCIVHLDITSLDIQQVVQVCWENQLYDAVIYVFNRGMNDYTTPMEKLFAVIVPPVREGKSLTDVEVVMGNKLLVYISCCLAGRAYPLGDIPEDLVVQVKHQVFEFLIRRHSADSSEEEEVFPFIRTLLHFDTREFLNVLAMTFEDFKNDKQALEYQQRIVDILLQVMVDNPDFTPSQVGGLFTFLARQLAKPDNTLFVNRKLFDQVLEFLCCPDDDSRHTERQQVLLELLQVGSVVQFNEERLLMLAEKAKFYQICEFLYEKQHLYDRIIDCYLRDPLRKGEVFSYIHNLLSMPGYSPEEKQSVMDKVRQHMHELVIFDPNKSADLVTFHFAEEVQQIISELQDDRLLFRFLSCLLEQQDGQHSGPVLLLQPELHELQLDLLSRFDPKHILNFLQTSQHYRLEEAVQITQKYHHNKATAFLLEKRGDVEGAFAVLLETLKENLSRVTAERAGDDEEETLTNVKDSLSDIISLCHQSSQSLNQQQREVLWFPLLETMMAAQKQVKGRDSKHTFEVLKELTMKVLNRMSSFISLPAIIQRILQDPVYGKGKLAEIQSLILGMLDTFNYEQTLLETTTSLLNHDLHWSLAHLRAAVTRGIHPRQDCCNICLQQYKRRQDAAAADEIIVFSCGHLYHLQCLQQKDSGWGFTSDVQQQWSCYKCSSSKGGRAASADSRGRSLSLAQTCVTSLQHDGATEADGKKGFVEVTLDHQQEQSWDQLRCLYRGPSRLSILSELSHSNEKTGLLIGSQTGTDHFQLKLAPPPLFEE

Target ORF information:

RefSeq Version	XM_011602840.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes vacuolar protein sorting 8 homolog (S. cerevisiae) (vps8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602840.1

ORF Insert Sequence:

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGACGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGATA CCAACCAGGC TCTGCGCCTC 
TGCCTGGGGA ACAAAGCAAC CGGGGCAGAT TACGGAGCTG TATCTGCCCT CAGCATCAAC 
CATGACTGCT CACGTCTGCT CTGTGGGTTT GCCAAGGGAC AGATTACAAT GTGGGATCTC 
GCCAATGGAA AACTCCTGAG AACTATTACT GATGCACACC CCCCAGGGAC GGCTATACTT 
CACGTGAAGT TTACAGACGA TCCAACTCTT GCAGTTTGCA ATGACAGTGG AGGATCCGTG 
TTTGAGCTGG CGTTCAGGAG AGTGATGGGG ATGCGCTCCT GTGACTCTCG ATGTCTCTTC 
AGCGGTTCCA AAGGGGAGGT GTGCTCTGTT GAACCCCTGC ATGCTCCTCC AGAGGCCAGA 
GATCACCCCA TCACCCACTA TTCTCTCCTG GCCATGGCCT CTCTCACTAA GATTCTGGTT 
ATTGGCCTGA AGCCCTCACT TAAAGTCTGG ATAACGTTCC CCTACAGTAA ATCTGATCCG 
TCCAGTGTTC CCCAGCTGGC CTGGCAGTTT GTTCCAGTCC AGAGAACCGT GAACCCCGTC 
CTGGCCTTTT CCAAAGGAGA TACAGTTCAC TTCCTCTTGG TAAAAAAGGA GGAAACAGGC 
ACCATCCATG TCATCAAACA AAGGCAGCTC CATGTCAGCT GTGACATCAT CAGCCTCTGT 
TGGATCAACA GCCGCACGCT GGTCTTGGTG GACAGCCATG AAAAACTGCA GGTTGTGGAC 
CGACCCAGTC AGGAGGTTTT GGAGACCCTC GACTTGGAAC AGGCTCTCGT TGGAGAAAAG 
GCCTGTTACC AGTCGGTCTC CAGCTACGCG GGACAGATTA TATATCTGGG CACCAAGTCT 
GCTCACATCA TGACTCTCAG GGCCTGGAGG GAGCGGATCG ACTGTCTGCT GAAGCTGGAA 
CATTTTCCCG AGGCCCTGTC TCTGGCCTGG TCGTTCCACG AGGGGACAGC TAAGGCTGTG 
GTCGGTCTGT TTGGAGACCC GGTGAAAAGA AAAGGAGTCG TTGCTGACAA GATGGTAGAG 
ATGATTTTCC AGTTTGCAGA CTTTGCCCTA AAGAAGTGTC CAGAACAAGG AAAGATCCAA 
GTGCTGGAAC AACACTTCCA TGAAGTGGTT CCAGTCCTGG TTGATTACTG TTTGCTGCTG 
AAGAGGCTTG ACCTGCTGTT TAACCAGCTG TACCCTCAAC TGGTGGAGAA CATGGTGGCT 
AAAGGCATCT TCTTGGAATC CCTGGAGTCC TACATTATCG CAGACCGCCT TGGTCACCTT 
CCCACACCGG TCATGAAAGA CCTTCTCATG CATTACCAAA GCAGCTGTAT GATGGAGAGC 
CTGGAGAGAT GCATCGTCCA TCTGGACATC ACCAGCCTGG ACATACAGCA GGTGGTTCAG 
GTGTGTTGGG AGAACCAACT CTACGATGCG GTGATCTACG TTTTCAATCG TGGCATGAAC 
GACTACACTA CACCTATGGA AAAACTCTTT GCAGTAATTG TCCCACCAGT AAGGGAGGGG 
AAGAGTCTTA CAGATGTGGA AGTGGTGATG GGAAACAAAC TCCTGGTGTA TATCAGCTGC 
TGTCTGGCAG GACGGGCATA TCCATTAGGA GACATCCCCG AAGACCTTGT GGTCCAGGTA 
AAGCATCAGG TGTTTGAATT TTTGATCCGT CGCCATTCAG CGGACTCCTC AGAGGAGGAA 
GAAGTGTTCC CGTTTATTCG CACTCTGCTC CACTTTGACA CTCGAGAGTT CCTCAACGTC 
CTCGCCATGA CATTCGAAGA CTTCAAGAAC GACAAACAGG CCCTTGAATA CCAGCAGAGG 
ATCGTGGACA TCTTACTGCA GGTGATGGTG GACAACCCAG ACTTTACTCC GTCCCAGGTG 
GGAGGACTCT TCACCTTTTT AGCGCGACAG TTGGCCAAAC CAGACAACAC ACTTTTCGTA 
AACAGGAAGC TCTTCGATCA GGTCCTGGAG TTTCTGTGCT GCCCAGATGA CGACTCCCGT 
CACACTGAGA GGCAGCAGGT TCTGTTGGAG CTGCTGCAGG TTGGGAGCGT GGTCCAGTTT 
AATGAAGAGA GGCTTCTGAT GCTGGCCGAG AAAGCCAAAT TCTACCAGAT ATGTGAATTT 
CTTTATGAGA AGCAACATCT CTACGACAGG ATCATTGACT GTTACCTGAG AGACCCCCTC 
AGAAAGGGAG AGGTCTTCAG CTACATCCAC AATCTGCTGT CCATGCCTGG TTACAGCCCG 
GAGGAGAAGC AGTCAGTCAT GGATAAAGTT CGGCAGCATA TGCATGAGCT GGTGATCTTT 
GACCCCAATA AATCAGCTGA TTTAGTGACG TTTCACTTTG CTGAAGAGGT CCAGCAAATA 
ATCTCTGAGC TTCAGGATGA TCGGCTGCTC TTCAGGTTCC TCAGCTGTCT CTTGGAGCAA 
CAGGACGGCC AACATTCGGG CCCCGTCCTA CTGCTGCAAC CTGAACTCCA TGAGCTTCAG 
CTGGACCTGC TCAGCCGTTT TGACCCCAAA CACATTTTGA ATTTCCTCCA GACCTCCCAA 
CACTATAGAC TAGAAGAGGC AGTACAGATA ACACAGAAGT ATCACCACAA CAAAGCTACA 
GCCTTTCTTT TGGAGAAGAG GGGCGATGTC GAAGGAGCTT TTGCTGTGCT GTTGGAGACG 
CTAAAGGAAA ACCTGAGTCG TGTCACAGCT GAGAGAGCAG GAGATGATGA AGAGGAAACC 
CTGACAAATG TCAAGGATTC TCTAAGTGAC ATCATCTCCT TGTGTCACCA GAGCTCTCAG 
AGCCTTAACC AGCAGCAAAG AGAGGTCCTG TGGTTTCCAC TGTTGGAGAC CATGATGGCT 
GCACAAAAAC AAGTGAAGGG ACGGGACAGT AAACACACAT TTGAGGTGCT GAAGGAGCTG 
ACGATGAAGG TCCTCAACCG TATGAGCAGC TTCATTTCTC TTCCCGCCAT CATCCAGCGG 
ATACTCCAGG ATCCAGTCTA CGGGAAAGGA AAGCTGGCAG AAATCCAGAG CCTTATTTTG 
GGCATGCTCG ACACTTTCAA CTATGAGCAG ACGTTACTTG AAACAACCAC CAGTTTGTTG 
AACCATGACC TCCACTGGTC CCTGGCGCAC TTGCGCGCAG CGGTCACTAG GGGGATCCAC 
CCACGACAGG ACTGCTGCAA CATCTGCCTG CAGCAGTACA AGAGGAGGCA GGATGCAGCA 
GCAGCAGACG AGATCATCGT CTTCAGCTGT GGGCATCTGT ACCACCTCCA GTGTCTGCAG 
CAGAAGGACT CCGGCTGGGG TTTTACGTCA GATGTTCAGC AGCAATGGAG CTGCTACAAA 
TGTTCCTCCA GCAAAGGAGG CCGAGCGGCG TCGGCTGATT CGAGAGGCCG CAGCTTGTCC 
CTGGCGCAGA CCTGCGTTAC ATCATTACAA CACGATGGGG CAACAGAAGC TGACGGGAAG 
AAGGGGTTTG TCGAAGTCAC CTTAGACCAT CAGCAAGAAC AGTCCTGGGA TCAGCTACGC 
TGCCTTTACA GAGGACCGTC CAGGCTGTCC ATCCTGTCCG AACTTTCACA CAGTAACGAG 
AAGACGGGCC TTCTGATCGG CAGCCAGACG GGGACAGATC ACTTCCAGCT GAAACTGGCC 
CCCCCGCCTC TGTTCGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078974 ( XM_029835098.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029690958.1 Takifugu rubripes VPS8 subunit of CORVET complex (LOC101078974), transcript variant X1, mRNA.

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CloneID	OTo65165
Clone ID Related Accession (Same CDS sequence)	XM_029835098.1
Accession Version	XM_029835098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGATGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGGAA AAGATACCAA CCAGGCTCTG 
CGCCTCTGCC TGGGGAACAA AGCAACCGGG GCAGATTACG GAGCTGTATC TGCCCTCAGC 
ATCAACCATG ACTGCTCACG TCTGCTCTGT GGGTTTGCCA AGGGACAGAT TACAATGTGG 
GATCTCGCCA ATGGAAAACT CCTGAGAACT ATTACTGATG CACACCCCCC AGGGACGGCT 
ATACTTCACG TGAAGTTTAC AGACGATCCA ACTCTTGCAG TTTGCAATGA CAGTGGAGGA 
TCCGTGTTTG AGCTGGCGTT CAGGAGAGTG ATGGGGATGC GCTCCTGTGA CTCTCGATGT 
CTCTTCAGCG GTTCCAAAGG GGAGGTGTGC TCTGTTGAAC CCCTGCATGC TCCTCCAGAG 
GCCAGAGATC ACCCCATCAC CCACTATTCT CTCCTGGCCA TGGCCTCTCT CACTAAGATT 
CTGGTTATTG GCCTGAAGCC CTCACTTAAA GTCTGGATAA CGTTCCCCTA CAGTAAATCT 
GATCCGTCCA GTGTTCCCCA GCTGGCCTGG CAGTTTGTTC CAGTCCAGAG AACCGTGAAC 
CCCGTCCTGG CCTTTTCCAA AGGAGATACA GTTCACTTCC TCTTGGTAAA AAAGGAGGAA 
ACGGGCACCA TCCATGTAAT CAAACAAAGG CAGCTCCATG TCAGCTGTGA CATCATCAGC 
CTCTGTTGGA TCAACAGCCG CACGCTGGTC TTGGTGGACA GCCATGAAAA ACTGCAGGTT 
GTGGACCGAC CCAGTCAGGA GGTTTTGGAG ACCCTCGACT TGGAACAGGT GCAGCTGGTT 
TATAACAGCA GACATTTCAA ATCGCTGGCC ACTGGAGGGA ATGTCTCCCA GGCTCTGGCT 
CTCGTTGGAG AAAAGGCCTG TTACCAGTCG GTCTCCAGCT ACGCGGGACA GATTATATAT 
CTGGGCACCA AGTCTGCTCA CATCATGACT CTCAGGGCCT GGAGGGAGCG GATCGACTGT 
CTGCTGAAGC TGGAACATTT TCCCGAGGCC CTGTCTCTGG CCTGGTCGTT CCACGAGGGG 
ACAGCTAAGG CTGTGGTCGG TCTGTTTGGA GACCCGGTGA AAAGAAAAGG AGTCGTTGCT 
GACAAGATGG TAGAGATGAT TTTCCAGTTT GCAGACTTTG CCCTAAAGAA GTGTCCAGAA 
CAAGGAAAGA TCCAAGTGCT GGAACAACAC TTCCATGAAG TTGTTCCAGT CCTGGTTGAT 
TACTGTTTGC TGCTGAAGAG GCTTGACCTG CTGTTTAACC AGCTGTACCC TCAACTGGTG 
GAGAACATGG TGGCTAAAGG CATCTTCTTG GAATCCCTGG AGTCCTACAT TATCGCAGAC 
CGCCTTGGTC ACCTTCCCAC ACCGGTCATG AAAGACCTTC TCATGCATTA CCAAAGCAGC 
TGTATGATGG AGAGCCTGGA GAGATGCATC GTCCATCTGG ACATCACCAG CCTGGACATA 
CAGCAGGTGG TTCAGGTGTG TTGGGAGAAC CAACTCTACG ATGCGGTGAT CTACGTTTTC 
AATCGTGGCA TGAACGACTA CACTACACCT ATGGAAAAAC TCTTTGCAGT AATTGTCCCA 
CCAGTAAGGG AGGGGAAGAG TCTTACAGAT GTGGAAGTGG TGATGGGAAA CAAACTCCTG 
GTGTATATCA GCTGCTGTCT GGCAGGACGG GCATATCCAT TAGGAGACAT CCCTGAAGAC 
CTTGTGGTCC AGGTAAAGCA TCAGGTGTTT GAATTTTTGA TCCGTCGCCA TTCAGCGGAC 
TCCTCAGAGG AGGAAGAAGT GTTCCCGTTT ATTCGCACTC TGCTCCACTT TGACACTCGA 
GAGTTCCTCA ACGTCCTCGC CATGACATTC GAAGACTTCA AGAACGACAA ACAGGCCCTT 
GAATACCAGC AGAGGATCGT GGACATCTTA CTGCAGGTGA TGGTGGACAA CCCAGACTTT 
ACTCCGTCCC AGGTGGGAGG ACTCTTCACC TTTTTAGCGC GACAGTTGGC CAAACCAGAC 
AACACACTTT TCGTAAACAG GAAGCTCTTC GATCAGGTCC TGGAGTTTCT GTGCTGCCCA 
GATGACGACT CCCGTCACAC TGAGAGGCAG CAGGTTCTGT TGGAGCTGCT GCAGGTTGGG 
AGCGTGGTCC AGTTTAATGA AGAGAGGCTT CTGATGCTGG CCGAGAAAGC CAAATTCTAC 
CAGATATGTG AATTTCTTTA TGAGAAGCAA CATCTCTACG ACAGGATCAT TGACTGTTAC 
CTGAGAGACC CCCTCAGAAA GGGAGAGGTC TTCAGCTACA TCCACAATCT GCTGTCCATG 
CCTGGTTACA GCCCGGAGGA GAAGCAGTCA GTCATGGATA AAGTTCGGCA GCATATGCAT 
GAGCTGGTGA TCTTTGACCC CAATAAATCA GCTGATTTAG TGACGTTTCA CTTTGCTGAA 
GAGGTCCAGC AAATAATCTC TGAGCTTCAG GATGATCGGC TGCTCTTCAG GTTCCTCAGC 
TGTCTCTTGG AGCAACAGGA CGGCCAACAT TCGGGCCCCG TCCTACTGCT GCAACCTGAA 
CTCCACGAGC TTCAGCTGGA CCTGCTCAGC CGTTTTGACC CCAAACACAT TTTGAATTTC 
CTCCAGACCT CCCAACACTA TAGACTAGAA GAGGCAGTAC AGATAACACA GAAGTATCAC 
CACAACAAAG CTACAGCCTT TCTTTTGGAG AAGAGGGGCG ATGTCGAAGG AGCTTTTGCT 
GTGCTGTTGG AGACGCTAAA GGAAAACCTG AGTCGTGTCA CAGCTGAGAG AGCAGGAGAT 
GATGAAGAGG AAACCCTGAC AAATGTCAAG GATTCTCTAA GTGACATCAT CTCCTTGTGT 
CACCAGAGCT CTCAGAGCCT TAACCAGCAG CAAAGAGAGG TCCTGTGGTT TCCACTGTTG 
GAGACCATGA TGGCTGCACA AAAACAAGTG AAGGGACGGG ACAGTAAACA CACATTTGAG 
GTGCTGAAGG AGCTGACGAT GAAGGTCCTC AACCGTATGA GCAGCTTCAT TTCTCTTCCC 
GCCATCATCC AGCGGATACT CCAGGATCCA GTCTACGGGA AAGGAAAGCT GGCAGAAATC 
CAGAGCCTTA TTTTGGGCAT GCTCGACACT TTCAACTATG AGCAGACGTT ACTTGAAACA 
ACCACCAGTT TGTTGAACCA TGACCTCCAC TGGTCCCTGG CGCACTTGCG CGCAGCGGTC 
ACTAGGGGGA TCCACCCACG ACAGGACTGC TGCAACATCT GCCTGCAGCA GTACAAGAGG 
AGGCAGGATG CAGCAGCAGC AGACGAGATC ATCGTCTTCA GCTGTGGGCA TCTGTACCAC 
CTCCAGTGTC TGCAGCAGAA GGACTCTGGC TGGGGTTTTA CGTCAGATGT TCAGCAGCAA 
TGGAGCTGCT ACAAATGTTC CTCCAGCAAA GGAGGCCGAG CGGCGTCGGC TGATTCGAGA 
GGCCGCAGCT TGTCCCTGGC GCAGACCTGC GTTACATCAT TACAACACGA TGGGGCAACA 
GAAGCTGACG GGAAGAAGGG GTTTGTCGAA GTCACCTTAG ACCATCAGCA AGAACAGTCC 
TGGGATCAGC TACGCTGCCT TTACAGAGGA CCGTCCAGGC TGTCCATCCT GTCCGAACTT 
TCACACAGTA ACGAGAAGAC GGGCCTTCTG ATCGGCAGCC AGACGGGGAC AGATCACTTC 
CAGCTGAAAC TGGCCCCCCC GCCTCTGTTC GAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029690958.1
CDS	42..4220
Translation	MSDSPDARSLLSASSQLNLMEVDAIDDKEFDIPQVDTPPTLESILNELEDEEEPFVLEDTCVLNTDNMDAHSCDTSSLASSDSGDPVHHKRRRRTVDMKPTLHGSVLRHSLLKGISAQIISAADKVDAGLPTAITMSGVIVVGTSHGLALVFGKDTNQALRLCLGNKATGADYGAVSALSINHDCSRLLCGFAKGQITMWDLANGKLLRTITDAHPPGTAILHVKFTDDPTLAVCNDSGGSVFELAFRRVMGMRSCDSRCLFSGSKGEVCSVEPLHAPPEARDHPITHYSLLAMASLTKILVIGLKPSLKVWITFPYSKSDPSSVPQLAWQFVPVQRTVNPVLAFSKGDTVHFLLVKKEETGTIHVIKQRQLHVSCDIISLCWINSRTLVLVDSHEKLQVVDRPSQEVLETLDLEQVQLVYNSRHFKSLATGGNVSQALALVGEKACYQSVSSYAGQIIYLGTKSAHIMTLRAWRERIDCLLKLEHFPEALSLAWSFHEGTAKAVVGLFGDPVKRKGVVADKMVEMIFQFADFALKKCPEQGKIQVLEQHFHEVVPVLVDYCLLLKRLDLLFNQLYPQLVENMVAKGIFLESLESYIIADRLGHLPTPVMKDLLMHYQSSCMMESLERCIVHLDITSLDIQQVVQVCWENQLYDAVIYVFNRGMNDYTTPMEKLFAVIVPPVREGKSLTDVEVVMGNKLLVYISCCLAGRAYPLGDIPEDLVVQVKHQVFEFLIRRHSADSSEEEEVFPFIRTLLHFDTREFLNVLAMTFEDFKNDKQALEYQQRIVDILLQVMVDNPDFTPSQVGGLFTFLARQLAKPDNTLFVNRKLFDQVLEFLCCPDDDSRHTERQQVLLELLQVGSVVQFNEERLLMLAEKAKFYQICEFLYEKQHLYDRIIDCYLRDPLRKGEVFSYIHNLLSMPGYSPEEKQSVMDKVRQHMHELVIFDPNKSADLVTFHFAEEVQQIISELQDDRLLFRFLSCLLEQQDGQHSGPVLLLQPELHELQLDLLSRFDPKHILNFLQTSQHYRLEEAVQITQKYHHNKATAFLLEKRGDVEGAFAVLLETLKENLSRVTAERAGDDEEETLTNVKDSLSDIISLCHQSSQSLNQQQREVLWFPLLETMMAAQKQVKGRDSKHTFEVLKELTMKVLNRMSSFISLPAIIQRILQDPVYGKGKLAEIQSLILGMLDTFNYEQTLLETTTSLLNHDLHWSLAHLRAAVTRGIHPRQDCCNICLQQYKRRQDAAAADEIIVFSCGHLYHLQCLQQKDSGWGFTSDVQQQWSCYKCSSSKGGRAASADSRGRSLSLAQTCVTSLQHDGATEADGKKGFVEVTLDHQQEQSWDQLRCLYRGPSRLSILSELSHSNEKTGLLIGSQTGTDHFQLKLAPPPLFEE

Target ORF information:

RefSeq Version	XM_029835098.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes VPS8 subunit of CORVET complex (LOC101078974), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029835098.1

ORF Insert Sequence:

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGATGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGGAA AAGATACCAA CCAGGCTCTG 
CGCCTCTGCC TGGGGAACAA AGCAACCGGG GCAGATTACG GAGCTGTATC TGCCCTCAGC 
ATCAACCATG ACTGCTCACG TCTGCTCTGT GGGTTTGCCA AGGGACAGAT TACAATGTGG 
GATCTCGCCA ATGGAAAACT CCTGAGAACT ATTACTGATG CACACCCCCC AGGGACGGCT 
ATACTTCACG TGAAGTTTAC AGACGATCCA ACTCTTGCAG TTTGCAATGA CAGTGGAGGA 
TCCGTGTTTG AGCTGGCGTT CAGGAGAGTG ATGGGGATGC GCTCCTGTGA CTCTCGATGT 
CTCTTCAGCG GTTCCAAAGG GGAGGTGTGC TCTGTTGAAC CCCTGCATGC TCCTCCAGAG 
GCCAGAGATC ACCCCATCAC CCACTATTCT CTCCTGGCCA TGGCCTCTCT CACTAAGATT 
CTGGTTATTG GCCTGAAGCC CTCACTTAAA GTCTGGATAA CGTTCCCCTA CAGTAAATCT 
GATCCGTCCA GTGTTCCCCA GCTGGCCTGG CAGTTTGTTC CAGTCCAGAG AACCGTGAAC 
CCCGTCCTGG CCTTTTCCAA AGGAGATACA GTTCACTTCC TCTTGGTAAA AAAGGAGGAA 
ACGGGCACCA TCCATGTAAT CAAACAAAGG CAGCTCCATG TCAGCTGTGA CATCATCAGC 
CTCTGTTGGA TCAACAGCCG CACGCTGGTC TTGGTGGACA GCCATGAAAA ACTGCAGGTT 
GTGGACCGAC CCAGTCAGGA GGTTTTGGAG ACCCTCGACT TGGAACAGGT GCAGCTGGTT 
TATAACAGCA GACATTTCAA ATCGCTGGCC ACTGGAGGGA ATGTCTCCCA GGCTCTGGCT 
CTCGTTGGAG AAAAGGCCTG TTACCAGTCG GTCTCCAGCT ACGCGGGACA GATTATATAT 
CTGGGCACCA AGTCTGCTCA CATCATGACT CTCAGGGCCT GGAGGGAGCG GATCGACTGT 
CTGCTGAAGC TGGAACATTT TCCCGAGGCC CTGTCTCTGG CCTGGTCGTT CCACGAGGGG 
ACAGCTAAGG CTGTGGTCGG TCTGTTTGGA GACCCGGTGA AAAGAAAAGG AGTCGTTGCT 
GACAAGATGG TAGAGATGAT TTTCCAGTTT GCAGACTTTG CCCTAAAGAA GTGTCCAGAA 
CAAGGAAAGA TCCAAGTGCT GGAACAACAC TTCCATGAAG TTGTTCCAGT CCTGGTTGAT 
TACTGTTTGC TGCTGAAGAG GCTTGACCTG CTGTTTAACC AGCTGTACCC TCAACTGGTG 
GAGAACATGG TGGCTAAAGG CATCTTCTTG GAATCCCTGG AGTCCTACAT TATCGCAGAC 
CGCCTTGGTC ACCTTCCCAC ACCGGTCATG AAAGACCTTC TCATGCATTA CCAAAGCAGC 
TGTATGATGG AGAGCCTGGA GAGATGCATC GTCCATCTGG ACATCACCAG CCTGGACATA 
CAGCAGGTGG TTCAGGTGTG TTGGGAGAAC CAACTCTACG ATGCGGTGAT CTACGTTTTC 
AATCGTGGCA TGAACGACTA CACTACACCT ATGGAAAAAC TCTTTGCAGT AATTGTCCCA 
CCAGTAAGGG AGGGGAAGAG TCTTACAGAT GTGGAAGTGG TGATGGGAAA CAAACTCCTG 
GTGTATATCA GCTGCTGTCT GGCAGGACGG GCATATCCAT TAGGAGACAT CCCTGAAGAC 
CTTGTGGTCC AGGTAAAGCA TCAGGTGTTT GAATTTTTGA TCCGTCGCCA TTCAGCGGAC 
TCCTCAGAGG AGGAAGAAGT GTTCCCGTTT ATTCGCACTC TGCTCCACTT TGACACTCGA 
GAGTTCCTCA ACGTCCTCGC CATGACATTC GAAGACTTCA AGAACGACAA ACAGGCCCTT 
GAATACCAGC AGAGGATCGT GGACATCTTA CTGCAGGTGA TGGTGGACAA CCCAGACTTT 
ACTCCGTCCC AGGTGGGAGG ACTCTTCACC TTTTTAGCGC GACAGTTGGC CAAACCAGAC 
AACACACTTT TCGTAAACAG GAAGCTCTTC GATCAGGTCC TGGAGTTTCT GTGCTGCCCA 
GATGACGACT CCCGTCACAC TGAGAGGCAG CAGGTTCTGT TGGAGCTGCT GCAGGTTGGG 
AGCGTGGTCC AGTTTAATGA AGAGAGGCTT CTGATGCTGG CCGAGAAAGC CAAATTCTAC 
CAGATATGTG AATTTCTTTA TGAGAAGCAA CATCTCTACG ACAGGATCAT TGACTGTTAC 
CTGAGAGACC CCCTCAGAAA GGGAGAGGTC TTCAGCTACA TCCACAATCT GCTGTCCATG 
CCTGGTTACA GCCCGGAGGA GAAGCAGTCA GTCATGGATA AAGTTCGGCA GCATATGCAT 
GAGCTGGTGA TCTTTGACCC CAATAAATCA GCTGATTTAG TGACGTTTCA CTTTGCTGAA 
GAGGTCCAGC AAATAATCTC TGAGCTTCAG GATGATCGGC TGCTCTTCAG GTTCCTCAGC 
TGTCTCTTGG AGCAACAGGA CGGCCAACAT TCGGGCCCCG TCCTACTGCT GCAACCTGAA 
CTCCACGAGC TTCAGCTGGA CCTGCTCAGC CGTTTTGACC CCAAACACAT TTTGAATTTC 
CTCCAGACCT CCCAACACTA TAGACTAGAA GAGGCAGTAC AGATAACACA GAAGTATCAC 
CACAACAAAG CTACAGCCTT TCTTTTGGAG AAGAGGGGCG ATGTCGAAGG AGCTTTTGCT 
GTGCTGTTGG AGACGCTAAA GGAAAACCTG AGTCGTGTCA CAGCTGAGAG AGCAGGAGAT 
GATGAAGAGG AAACCCTGAC AAATGTCAAG GATTCTCTAA GTGACATCAT CTCCTTGTGT 
CACCAGAGCT CTCAGAGCCT TAACCAGCAG CAAAGAGAGG TCCTGTGGTT TCCACTGTTG 
GAGACCATGA TGGCTGCACA AAAACAAGTG AAGGGACGGG ACAGTAAACA CACATTTGAG 
GTGCTGAAGG AGCTGACGAT GAAGGTCCTC AACCGTATGA GCAGCTTCAT TTCTCTTCCC 
GCCATCATCC AGCGGATACT CCAGGATCCA GTCTACGGGA AAGGAAAGCT GGCAGAAATC 
CAGAGCCTTA TTTTGGGCAT GCTCGACACT TTCAACTATG AGCAGACGTT ACTTGAAACA 
ACCACCAGTT TGTTGAACCA TGACCTCCAC TGGTCCCTGG CGCACTTGCG CGCAGCGGTC 
ACTAGGGGGA TCCACCCACG ACAGGACTGC TGCAACATCT GCCTGCAGCA GTACAAGAGG 
AGGCAGGATG CAGCAGCAGC AGACGAGATC ATCGTCTTCA GCTGTGGGCA TCTGTACCAC 
CTCCAGTGTC TGCAGCAGAA GGACTCTGGC TGGGGTTTTA CGTCAGATGT TCAGCAGCAA 
TGGAGCTGCT ACAAATGTTC CTCCAGCAAA GGAGGCCGAG CGGCGTCGGC TGATTCGAGA 
GGCCGCAGCT TGTCCCTGGC GCAGACCTGC GTTACATCAT TACAACACGA TGGGGCAACA 
GAAGCTGACG GGAAGAAGGG GTTTGTCGAA GTCACCTTAG ACCATCAGCA AGAACAGTCC 
TGGGATCAGC TACGCTGCCT TTACAGAGGA CCGTCCAGGC TGTCCATCCT GTCCGAACTT 
TCACACAGTA ACGAGAAGAC GGGCCTTCTG ATCGGCAGCC AGACGGGGAC AGATCACTTC 
CAGCTGAAAC TGGCCCCCCC GCCTCTGTTC GAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078974 ( XM_011602839.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601141.1 Takifugu rubripes VPS8 subunit of CORVET complex (LOC101078974), transcript variant X2, mRNA.

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CloneID	OTo65166
Clone ID Related Accession (Same CDS sequence)	XM_011602839.2
Accession Version	XM_011602839.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011602839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGATGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGATA CCAACCAGGC TCTGCGCCTC 
TGCCTGGGGA ACAAAGCAAC CGGGGCAGAT TACGGAGCTG TATCTGCCCT CAGCATCAAC 
CATGACTGCT CACGTCTGCT CTGTGGGTTT GCCAAGGGAC AGATTACAAT GTGGGATCTC 
GCCAATGGAA AACTCCTGAG AACTATTACT GATGCACACC CCCCAGGGAC GGCTATACTT 
CACGTGAAGT TTACAGACGA TCCAACTCTT GCAGTTTGCA ATGACAGTGG AGGATCCGTG 
TTTGAGCTGG CGTTCAGGAG AGTGATGGGG ATGCGCTCCT GTGACTCTCG ATGTCTCTTC 
AGCGGTTCCA AAGGGGAGGT GTGCTCTGTT GAACCCCTGC ATGCTCCTCC AGAGGCCAGA 
GATCACCCCA TCACCCACTA TTCTCTCCTG GCCATGGCCT CTCTCACTAA GATTCTGGTT 
ATTGGCCTGA AGCCCTCACT TAAAGTCTGG ATAACGTTCC CCTACAGTAA ATCTGATCCG 
TCCAGTGTTC CCCAGCTGGC CTGGCAGTTT GTTCCAGTCC AGAGAACCGT GAACCCCGTC 
CTGGCCTTTT CCAAAGGAGA TACAGTTCAC TTCCTCTTGG TAAAAAAGGA GGAAACGGGC 
ACCATCCATG TAATCAAACA AAGGCAGCTC CATGTCAGCT GTGACATCAT CAGCCTCTGT 
TGGATCAACA GCCGCACGCT GGTCTTGGTG GACAGCCATG AAAAACTGCA GGTTGTGGAC 
CGACCCAGTC AGGAGGTTTT GGAGACCCTC GACTTGGAAC AGGTGCAGCT GGTTTATAAC 
AGCAGACATT TCAAATCGCT GGCCACTGGA GGGAATGTCT CCCAGGCTCT GGCTCTCGTT 
GGAGAAAAGG CCTGTTACCA GTCGGTCTCC AGCTACGCGG GACAGATTAT ATATCTGGGC 
ACCAAGTCTG CTCACATCAT GACTCTCAGG GCCTGGAGGG AGCGGATCGA CTGTCTGCTG 
AAGCTGGAAC ATTTTCCCGA GGCCCTGTCT CTGGCCTGGT CGTTCCACGA GGGGACAGCT 
AAGGCTGTGG TCGGTCTGTT TGGAGACCCG GTGAAAAGAA AAGGAGTCGT TGCTGACAAG 
ATGGTAGAGA TGATTTTCCA GTTTGCAGAC TTTGCCCTAA AGAAGTGTCC AGAACAAGGA 
AAGATCCAAG TGCTGGAACA ACACTTCCAT GAAGTTGTTC CAGTCCTGGT TGATTACTGT 
TTGCTGCTGA AGAGGCTTGA CCTGCTGTTT AACCAGCTGT ACCCTCAACT GGTGGAGAAC 
ATGGTGGCTA AAGGCATCTT CTTGGAATCC CTGGAGTCCT ACATTATCGC AGACCGCCTT 
GGTCACCTTC CCACACCGGT CATGAAAGAC CTTCTCATGC ATTACCAAAG CAGCTGTATG 
ATGGAGAGCC TGGAGAGATG CATCGTCCAT CTGGACATCA CCAGCCTGGA CATACAGCAG 
GTGGTTCAGG TGTGTTGGGA GAACCAACTC TACGATGCGG TGATCTACGT TTTCAATCGT 
GGCATGAACG ACTACACTAC ACCTATGGAA AAACTCTTTG CAGTAATTGT CCCACCAGTA 
AGGGAGGGGA AGAGTCTTAC AGATGTGGAA GTGGTGATGG GAAACAAACT CCTGGTGTAT 
ATCAGCTGCT GTCTGGCAGG ACGGGCATAT CCATTAGGAG ACATCCCTGA AGACCTTGTG 
GTCCAGGTAA AGCATCAGGT GTTTGAATTT TTGATCCGTC GCCATTCAGC GGACTCCTCA 
GAGGAGGAAG AAGTGTTCCC GTTTATTCGC ACTCTGCTCC ACTTTGACAC TCGAGAGTTC 
CTCAACGTCC TCGCCATGAC ATTCGAAGAC TTCAAGAACG ACAAACAGGC CCTTGAATAC 
CAGCAGAGGA TCGTGGACAT CTTACTGCAG GTGATGGTGG ACAACCCAGA CTTTACTCCG 
TCCCAGGTGG GAGGACTCTT CACCTTTTTA GCGCGACAGT TGGCCAAACC AGACAACACA 
CTTTTCGTAA ACAGGAAGCT CTTCGATCAG GTCCTGGAGT TTCTGTGCTG CCCAGATGAC 
GACTCCCGTC ACACTGAGAG GCAGCAGGTT CTGTTGGAGC TGCTGCAGGT TGGGAGCGTG 
GTCCAGTTTA ATGAAGAGAG GCTTCTGATG CTGGCCGAGA AAGCCAAATT CTACCAGATA 
TGTGAATTTC TTTATGAGAA GCAACATCTC TACGACAGGA TCATTGACTG TTACCTGAGA 
GACCCCCTCA GAAAGGGAGA GGTCTTCAGC TACATCCACA ATCTGCTGTC CATGCCTGGT 
TACAGCCCGG AGGAGAAGCA GTCAGTCATG GATAAAGTTC GGCAGCATAT GCATGAGCTG 
GTGATCTTTG ACCCCAATAA ATCAGCTGAT TTAGTGACGT TTCACTTTGC TGAAGAGGTC 
CAGCAAATAA TCTCTGAGCT TCAGGATGAT CGGCTGCTCT TCAGGTTCCT CAGCTGTCTC 
TTGGAGCAAC AGGACGGCCA ACATTCGGGC CCCGTCCTAC TGCTGCAACC TGAACTCCAC 
GAGCTTCAGC TGGACCTGCT CAGCCGTTTT GACCCCAAAC ACATTTTGAA TTTCCTCCAG 
ACCTCCCAAC ACTATAGACT AGAAGAGGCA GTACAGATAA CACAGAAGTA TCACCACAAC 
AAAGCTACAG CCTTTCTTTT GGAGAAGAGG GGCGATGTCG AAGGAGCTTT TGCTGTGCTG 
TTGGAGACGC TAAAGGAAAA CCTGAGTCGT GTCACAGCTG AGAGAGCAGG AGATGATGAA 
GAGGAAACCC TGACAAATGT CAAGGATTCT CTAAGTGACA TCATCTCCTT GTGTCACCAG 
AGCTCTCAGA GCCTTAACCA GCAGCAAAGA GAGGTCCTGT GGTTTCCACT GTTGGAGACC 
ATGATGGCTG CACAAAAACA AGTGAAGGGA CGGGACAGTA AACACACATT TGAGGTGCTG 
AAGGAGCTGA CGATGAAGGT CCTCAACCGT ATGAGCAGCT TCATTTCTCT TCCCGCCATC 
ATCCAGCGGA TACTCCAGGA TCCAGTCTAC GGGAAAGGAA AGCTGGCAGA AATCCAGAGC 
CTTATTTTGG GCATGCTCGA CACTTTCAAC TATGAGCAGA CGTTACTTGA AACAACCACC 
AGTTTGTTGA ACCATGACCT CCACTGGTCC CTGGCGCACT TGCGCGCAGC GGTCACTAGG 
GGGATCCACC CACGACAGGA CTGCTGCAAC ATCTGCCTGC AGCAGTACAA GAGGAGGCAG 
GATGCAGCAG CAGCAGACGA GATCATCGTC TTCAGCTGTG GGCATCTGTA CCACCTCCAG 
TGTCTGCAGC AGAAGGACTC TGGCTGGGGT TTTACGTCAG ATGTTCAGCA GCAATGGAGC 
TGCTACAAAT GTTCCTCCAG CAAAGGAGGC CGAGCGGCGT CGGCTGATTC GAGAGGCCGC 
AGCTTGTCCC TGGCGCAGAC CTGCGTTACA TCATTACAAC ACGATGGGGC AACAGAAGCT 
GACGGGAAGA AGGGGTTTGT CGAAGTCACC TTAGACCATC AGCAAGAACA GTCCTGGGAT 
CAGCTACGCT GCCTTTACAG AGGACCGTCC AGGCTGTCCA TCCTGTCCGA ACTTTCACAC 
AGTAACGAGA AGACGGGCCT TCTGATCGGC AGCCAGACGG GGACAGATCA CTTCCAGCTG 
AAACTGGCCC CCCCGCCTCT GTTCGAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601141.1
CDS	42..4214
Translation	MSDSPDARSLLSASSQLNLMEVDAIDDKEFDIPQVDTPPTLESILNELEDEEEPFVLEDTCVLNTDNMDAHSCDTSSLASSDSGDPVHHKRRRRTVDMKPTLHGSVLRHSLLKGISAQIISAADKVDAGLPTAITMSGVIVVGTSHGLALVFDTNQALRLCLGNKATGADYGAVSALSINHDCSRLLCGFAKGQITMWDLANGKLLRTITDAHPPGTAILHVKFTDDPTLAVCNDSGGSVFELAFRRVMGMRSCDSRCLFSGSKGEVCSVEPLHAPPEARDHPITHYSLLAMASLTKILVIGLKPSLKVWITFPYSKSDPSSVPQLAWQFVPVQRTVNPVLAFSKGDTVHFLLVKKEETGTIHVIKQRQLHVSCDIISLCWINSRTLVLVDSHEKLQVVDRPSQEVLETLDLEQVQLVYNSRHFKSLATGGNVSQALALVGEKACYQSVSSYAGQIIYLGTKSAHIMTLRAWRERIDCLLKLEHFPEALSLAWSFHEGTAKAVVGLFGDPVKRKGVVADKMVEMIFQFADFALKKCPEQGKIQVLEQHFHEVVPVLVDYCLLLKRLDLLFNQLYPQLVENMVAKGIFLESLESYIIADRLGHLPTPVMKDLLMHYQSSCMMESLERCIVHLDITSLDIQQVVQVCWENQLYDAVIYVFNRGMNDYTTPMEKLFAVIVPPVREGKSLTDVEVVMGNKLLVYISCCLAGRAYPLGDIPEDLVVQVKHQVFEFLIRRHSADSSEEEEVFPFIRTLLHFDTREFLNVLAMTFEDFKNDKQALEYQQRIVDILLQVMVDNPDFTPSQVGGLFTFLARQLAKPDNTLFVNRKLFDQVLEFLCCPDDDSRHTERQQVLLELLQVGSVVQFNEERLLMLAEKAKFYQICEFLYEKQHLYDRIIDCYLRDPLRKGEVFSYIHNLLSMPGYSPEEKQSVMDKVRQHMHELVIFDPNKSADLVTFHFAEEVQQIISELQDDRLLFRFLSCLLEQQDGQHSGPVLLLQPELHELQLDLLSRFDPKHILNFLQTSQHYRLEEAVQITQKYHHNKATAFLLEKRGDVEGAFAVLLETLKENLSRVTAERAGDDEEETLTNVKDSLSDIISLCHQSSQSLNQQQREVLWFPLLETMMAAQKQVKGRDSKHTFEVLKELTMKVLNRMSSFISLPAIIQRILQDPVYGKGKLAEIQSLILGMLDTFNYEQTLLETTTSLLNHDLHWSLAHLRAAVTRGIHPRQDCCNICLQQYKRRQDAAAADEIIVFSCGHLYHLQCLQQKDSGWGFTSDVQQQWSCYKCSSSKGGRAASADSRGRSLSLAQTCVTSLQHDGATEADGKKGFVEVTLDHQQEQSWDQLRCLYRGPSRLSILSELSHSNEKTGLLIGSQTGTDHFQLKLAPPPLFEE

Target ORF information:

RefSeq Version	XM_011602839.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes VPS8 subunit of CORVET complex (LOC101078974), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602839.2

ORF Insert Sequence:

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGATGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGATA CCAACCAGGC TCTGCGCCTC 
TGCCTGGGGA ACAAAGCAAC CGGGGCAGAT TACGGAGCTG TATCTGCCCT CAGCATCAAC 
CATGACTGCT CACGTCTGCT CTGTGGGTTT GCCAAGGGAC AGATTACAAT GTGGGATCTC 
GCCAATGGAA AACTCCTGAG AACTATTACT GATGCACACC CCCCAGGGAC GGCTATACTT 
CACGTGAAGT TTACAGACGA TCCAACTCTT GCAGTTTGCA ATGACAGTGG AGGATCCGTG 
TTTGAGCTGG CGTTCAGGAG AGTGATGGGG ATGCGCTCCT GTGACTCTCG ATGTCTCTTC 
AGCGGTTCCA AAGGGGAGGT GTGCTCTGTT GAACCCCTGC ATGCTCCTCC AGAGGCCAGA 
GATCACCCCA TCACCCACTA TTCTCTCCTG GCCATGGCCT CTCTCACTAA GATTCTGGTT 
ATTGGCCTGA AGCCCTCACT TAAAGTCTGG ATAACGTTCC CCTACAGTAA ATCTGATCCG 
TCCAGTGTTC CCCAGCTGGC CTGGCAGTTT GTTCCAGTCC AGAGAACCGT GAACCCCGTC 
CTGGCCTTTT CCAAAGGAGA TACAGTTCAC TTCCTCTTGG TAAAAAAGGA GGAAACGGGC 
ACCATCCATG TAATCAAACA AAGGCAGCTC CATGTCAGCT GTGACATCAT CAGCCTCTGT 
TGGATCAACA GCCGCACGCT GGTCTTGGTG GACAGCCATG AAAAACTGCA GGTTGTGGAC 
CGACCCAGTC AGGAGGTTTT GGAGACCCTC GACTTGGAAC AGGTGCAGCT GGTTTATAAC 
AGCAGACATT TCAAATCGCT GGCCACTGGA GGGAATGTCT CCCAGGCTCT GGCTCTCGTT 
GGAGAAAAGG CCTGTTACCA GTCGGTCTCC AGCTACGCGG GACAGATTAT ATATCTGGGC 
ACCAAGTCTG CTCACATCAT GACTCTCAGG GCCTGGAGGG AGCGGATCGA CTGTCTGCTG 
AAGCTGGAAC ATTTTCCCGA GGCCCTGTCT CTGGCCTGGT CGTTCCACGA GGGGACAGCT 
AAGGCTGTGG TCGGTCTGTT TGGAGACCCG GTGAAAAGAA AAGGAGTCGT TGCTGACAAG 
ATGGTAGAGA TGATTTTCCA GTTTGCAGAC TTTGCCCTAA AGAAGTGTCC AGAACAAGGA 
AAGATCCAAG TGCTGGAACA ACACTTCCAT GAAGTTGTTC CAGTCCTGGT TGATTACTGT 
TTGCTGCTGA AGAGGCTTGA CCTGCTGTTT AACCAGCTGT ACCCTCAACT GGTGGAGAAC 
ATGGTGGCTA AAGGCATCTT CTTGGAATCC CTGGAGTCCT ACATTATCGC AGACCGCCTT 
GGTCACCTTC CCACACCGGT CATGAAAGAC CTTCTCATGC ATTACCAAAG CAGCTGTATG 
ATGGAGAGCC TGGAGAGATG CATCGTCCAT CTGGACATCA CCAGCCTGGA CATACAGCAG 
GTGGTTCAGG TGTGTTGGGA GAACCAACTC TACGATGCGG TGATCTACGT TTTCAATCGT 
GGCATGAACG ACTACACTAC ACCTATGGAA AAACTCTTTG CAGTAATTGT CCCACCAGTA 
AGGGAGGGGA AGAGTCTTAC AGATGTGGAA GTGGTGATGG GAAACAAACT CCTGGTGTAT 
ATCAGCTGCT GTCTGGCAGG ACGGGCATAT CCATTAGGAG ACATCCCTGA AGACCTTGTG 
GTCCAGGTAA AGCATCAGGT GTTTGAATTT TTGATCCGTC GCCATTCAGC GGACTCCTCA 
GAGGAGGAAG AAGTGTTCCC GTTTATTCGC ACTCTGCTCC ACTTTGACAC TCGAGAGTTC 
CTCAACGTCC TCGCCATGAC ATTCGAAGAC TTCAAGAACG ACAAACAGGC CCTTGAATAC 
CAGCAGAGGA TCGTGGACAT CTTACTGCAG GTGATGGTGG ACAACCCAGA CTTTACTCCG 
TCCCAGGTGG GAGGACTCTT CACCTTTTTA GCGCGACAGT TGGCCAAACC AGACAACACA 
CTTTTCGTAA ACAGGAAGCT CTTCGATCAG GTCCTGGAGT TTCTGTGCTG CCCAGATGAC 
GACTCCCGTC ACACTGAGAG GCAGCAGGTT CTGTTGGAGC TGCTGCAGGT TGGGAGCGTG 
GTCCAGTTTA ATGAAGAGAG GCTTCTGATG CTGGCCGAGA AAGCCAAATT CTACCAGATA 
TGTGAATTTC TTTATGAGAA GCAACATCTC TACGACAGGA TCATTGACTG TTACCTGAGA 
GACCCCCTCA GAAAGGGAGA GGTCTTCAGC TACATCCACA ATCTGCTGTC CATGCCTGGT 
TACAGCCCGG AGGAGAAGCA GTCAGTCATG GATAAAGTTC GGCAGCATAT GCATGAGCTG 
GTGATCTTTG ACCCCAATAA ATCAGCTGAT TTAGTGACGT TTCACTTTGC TGAAGAGGTC 
CAGCAAATAA TCTCTGAGCT TCAGGATGAT CGGCTGCTCT TCAGGTTCCT CAGCTGTCTC 
TTGGAGCAAC AGGACGGCCA ACATTCGGGC CCCGTCCTAC TGCTGCAACC TGAACTCCAC 
GAGCTTCAGC TGGACCTGCT CAGCCGTTTT GACCCCAAAC ACATTTTGAA TTTCCTCCAG 
ACCTCCCAAC ACTATAGACT AGAAGAGGCA GTACAGATAA CACAGAAGTA TCACCACAAC 
AAAGCTACAG CCTTTCTTTT GGAGAAGAGG GGCGATGTCG AAGGAGCTTT TGCTGTGCTG 
TTGGAGACGC TAAAGGAAAA CCTGAGTCGT GTCACAGCTG AGAGAGCAGG AGATGATGAA 
GAGGAAACCC TGACAAATGT CAAGGATTCT CTAAGTGACA TCATCTCCTT GTGTCACCAG 
AGCTCTCAGA GCCTTAACCA GCAGCAAAGA GAGGTCCTGT GGTTTCCACT GTTGGAGACC 
ATGATGGCTG CACAAAAACA AGTGAAGGGA CGGGACAGTA AACACACATT TGAGGTGCTG 
AAGGAGCTGA CGATGAAGGT CCTCAACCGT ATGAGCAGCT TCATTTCTCT TCCCGCCATC 
ATCCAGCGGA TACTCCAGGA TCCAGTCTAC GGGAAAGGAA AGCTGGCAGA AATCCAGAGC 
CTTATTTTGG GCATGCTCGA CACTTTCAAC TATGAGCAGA CGTTACTTGA AACAACCACC 
AGTTTGTTGA ACCATGACCT CCACTGGTCC CTGGCGCACT TGCGCGCAGC GGTCACTAGG 
GGGATCCACC CACGACAGGA CTGCTGCAAC ATCTGCCTGC AGCAGTACAA GAGGAGGCAG 
GATGCAGCAG CAGCAGACGA GATCATCGTC TTCAGCTGTG GGCATCTGTA CCACCTCCAG 
TGTCTGCAGC AGAAGGACTC TGGCTGGGGT TTTACGTCAG ATGTTCAGCA GCAATGGAGC 
TGCTACAAAT GTTCCTCCAG CAAAGGAGGC CGAGCGGCGT CGGCTGATTC GAGAGGCCGC 
AGCTTGTCCC TGGCGCAGAC CTGCGTTACA TCATTACAAC ACGATGGGGC AACAGAAGCT 
GACGGGAAGA AGGGGTTTGT CGAAGTCACC TTAGACCATC AGCAAGAACA GTCCTGGGAT 
CAGCTACGCT GCCTTTACAG AGGACCGTCC AGGCTGTCCA TCCTGTCCGA ACTTTCACAC 
AGTAACGAGA AGACGGGCCT TCTGATCGGC AGCCAGACGG GGACAGATCA CTTCCAGCTG 
AAACTGGCCC CCCCGCCTCT GTTCGAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078974 ( XM_029835099.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029690959.1 Takifugu rubripes VPS8 subunit of CORVET complex (LOC101078974), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo65167
Clone ID Related Accession (Same CDS sequence)	XM_029835099.1
Accession Version	XM_029835099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGATGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGGAA AAGATACCAA CCAGGCTCTG 
CGCCTCTGCC TGGGGAACAA AGCAACCGGG GCAGATTACG GAGCTGTATC TGCCCTCAGC 
ATCAACCATG ACTGCTCACG TCTGCTCTGT GGGTTTGCCA AGGGACAGAT TACAATGTGG 
GATCTCGCCA ATGGAAAACT CCTGAGAACT ATTACTGATG CACACCCCCC AGGGACGGCT 
ATACTTCACG TGAAGTTTAC AGACGATCCA ACTCTTGCAG TTTGCAATGA CAGTGGAGGA 
TCCGTGTTTG AGCTGGCGTT CAGGAGAGTG ATGGGGATGC GCTCCTGTGA CTCTCGATGT 
CTCTTCAGCG GTTCCAAAGG GGAGGTGTGC TCTGTTGAAC CCCTGCATGC TCCTCCAGAG 
GCCAGAGATC ACCCCATCAC CCACTATTCT CTCCTGGCCA TGGCCTCTCT CACTAAGATT 
CTGGTTATTG GCCTGAAGCC CTCACTTAAA GTCTGGATAA CGTTCCCCTA CAGTAAATCT 
GATCCGTCCA GTGTTCCCCA GCTGGCCTGG CAGTTTGTTC CAGTCCAGAG AACCGTGAAC 
CCCGTCCTGG CCTTTTCCAA AGGAGATACA GTTCACTTCC TCTTGGTAAA AAAGGAGGAA 
ACGGGCACCA TCCATGTAAT CAAACAAAGG CAGCTCCATG TCAGCTGTGA CATCATCAGC 
CTCTGTTGGA TCAACAGCCG CACGCTGGTC TTGGTGGACA GCCATGAAAA ACTGCAGGTT 
GTGGACCGAC CCAGTCAGGA GGTTTTGGAG ACCCTCGACT TGGAACAGGC TCTCGTTGGA 
GAAAAGGCCT GTTACCAGTC GGTCTCCAGC TACGCGGGAC AGATTATATA TCTGGGCACC 
AAGTCTGCTC ACATCATGAC TCTCAGGGCC TGGAGGGAGC GGATCGACTG TCTGCTGAAG 
CTGGAACATT TTCCCGAGGC CCTGTCTCTG GCCTGGTCGT TCCACGAGGG GACAGCTAAG 
GCTGTGGTCG GTCTGTTTGG AGACCCGGTG AAAAGAAAAG GAGTCGTTGC TGACAAGATG 
GTAGAGATGA TTTTCCAGTT TGCAGACTTT GCCCTAAAGA AGTGTCCAGA ACAAGGAAAG 
ATCCAAGTGC TGGAACAACA CTTCCATGAA GTTGTTCCAG TCCTGGTTGA TTACTGTTTG 
CTGCTGAAGA GGCTTGACCT GCTGTTTAAC CAGCTGTACC CTCAACTGGT GGAGAACATG 
GTGGCTAAAG GCATCTTCTT GGAATCCCTG GAGTCCTACA TTATCGCAGA CCGCCTTGGT 
CACCTTCCCA CACCGGTCAT GAAAGACCTT CTCATGCATT ACCAAAGCAG CTGTATGATG 
GAGAGCCTGG AGAGATGCAT CGTCCATCTG GACATCACCA GCCTGGACAT ACAGCAGGTG 
GTTCAGGTGT GTTGGGAGAA CCAACTCTAC GATGCGGTGA TCTACGTTTT CAATCGTGGC 
ATGAACGACT ACACTACACC TATGGAAAAA CTCTTTGCAG TAATTGTCCC ACCAGTAAGG 
GAGGGGAAGA GTCTTACAGA TGTGGAAGTG GTGATGGGAA ACAAACTCCT GGTGTATATC 
AGCTGCTGTC TGGCAGGACG GGCATATCCA TTAGGAGACA TCCCTGAAGA CCTTGTGGTC 
CAGGTAAAGC ATCAGGTGTT TGAATTTTTG ATCCGTCGCC ATTCAGCGGA CTCCTCAGAG 
GAGGAAGAAG TGTTCCCGTT TATTCGCACT CTGCTCCACT TTGACACTCG AGAGTTCCTC 
AACGTCCTCG CCATGACATT CGAAGACTTC AAGAACGACA AACAGGCCCT TGAATACCAG 
CAGAGGATCG TGGACATCTT ACTGCAGGTG ATGGTGGACA ACCCAGACTT TACTCCGTCC 
CAGGTGGGAG GACTCTTCAC CTTTTTAGCG CGACAGTTGG CCAAACCAGA CAACACACTT 
TTCGTAAACA GGAAGCTCTT CGATCAGGTC CTGGAGTTTC TGTGCTGCCC AGATGACGAC 
TCCCGTCACA CTGAGAGGCA GCAGGTTCTG TTGGAGCTGC TGCAGGTTGG GAGCGTGGTC 
CAGTTTAATG AAGAGAGGCT TCTGATGCTG GCCGAGAAAG CCAAATTCTA CCAGATATGT 
GAATTTCTTT ATGAGAAGCA ACATCTCTAC GACAGGATCA TTGACTGTTA CCTGAGAGAC 
CCCCTCAGAA AGGGAGAGGT CTTCAGCTAC ATCCACAATC TGCTGTCCAT GCCTGGTTAC 
AGCCCGGAGG AGAAGCAGTC AGTCATGGAT AAAGTTCGGC AGCATATGCA TGAGCTGGTG 
ATCTTTGACC CCAATAAATC AGCTGATTTA GTGACGTTTC ACTTTGCTGA AGAGGTCCAG 
CAAATAATCT CTGAGCTTCA GGATGATCGG CTGCTCTTCA GGTTCCTCAG CTGTCTCTTG 
GAGCAACAGG ACGGCCAACA TTCGGGCCCC GTCCTACTGC TGCAACCTGA ACTCCACGAG 
CTTCAGCTGG ACCTGCTCAG CCGTTTTGAC CCCAAACACA TTTTGAATTT CCTCCAGACC 
TCCCAACACT ATAGACTAGA AGAGGCAGTA CAGATAACAC AGAAGTATCA CCACAACAAA 
GCTACAGCCT TTCTTTTGGA GAAGAGGGGC GATGTCGAAG GAGCTTTTGC TGTGCTGTTG 
GAGACGCTAA AGGAAAACCT GAGTCGTGTC ACAGCTGAGA GAGCAGGAGA TGATGAAGAG 
GAAACCCTGA CAAATGTCAA GGATTCTCTA AGTGACATCA TCTCCTTGTG TCACCAGAGC 
TCTCAGAGCC TTAACCAGCA GCAAAGAGAG GTCCTGTGGT TTCCACTGTT GGAGACCATG 
ATGGCTGCAC AAAAACAAGT GAAGGGACGG GACAGTAAAC ACACATTTGA GGTGCTGAAG 
GAGCTGACGA TGAAGGTCCT CAACCGTATG AGCAGCTTCA TTTCTCTTCC CGCCATCATC 
CAGCGGATAC TCCAGGATCC AGTCTACGGG AAAGGAAAGC TGGCAGAAAT CCAGAGCCTT 
ATTTTGGGCA TGCTCGACAC TTTCAACTAT GAGCAGACGT TACTTGAAAC AACCACCAGT 
TTGTTGAACC ATGACCTCCA CTGGTCCCTG GCGCACTTGC GCGCAGCGGT CACTAGGGGG 
ATCCACCCAC GACAGGACTG CTGCAACATC TGCCTGCAGC AGTACAAGAG GAGGCAGGAT 
GCAGCAGCAG CAGACGAGAT CATCGTCTTC AGCTGTGGGC ATCTGTACCA CCTCCAGTGT 
CTGCAGCAGA AGGACTCTGG CTGGGGTTTT ACGTCAGATG TTCAGCAGCA ATGGAGCTGC 
TACAAATGTT CCTCCAGCAA AGGAGGCCGA GCGGCGTCGG CTGATTCGAG AGGCCGCAGC 
TTGTCCCTGG CGCAGACCTG CGTTACATCA TTACAACACG ATGGGGCAAC AGAAGCTGAC 
GGGAAGAAGG GGTTTGTCGA AGTCACCTTA GACCATCAGC AAGAACAGTC CTGGGATCAG 
CTACGCTGCC TTTACAGAGG ACCGTCCAGG CTGTCCATCC TGTCCGAACT TTCACACAGT 
AACGAGAAGA CGGGCCTTCT GATCGGCAGC CAGACGGGGA CAGATCACTT CCAGCTGAAA 
CTGGCCCCCC CGCCTCTGTT CGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029690959.1
CDS	42..4151
Translation	MSDSPDARSLLSASSQLNLMEVDAIDDKEFDIPQVDTPPTLESILNELEDEEEPFVLEDTCVLNTDNMDAHSCDTSSLASSDSGDPVHHKRRRRTVDMKPTLHGSVLRHSLLKGISAQIISAADKVDAGLPTAITMSGVIVVGTSHGLALVFGKDTNQALRLCLGNKATGADYGAVSALSINHDCSRLLCGFAKGQITMWDLANGKLLRTITDAHPPGTAILHVKFTDDPTLAVCNDSGGSVFELAFRRVMGMRSCDSRCLFSGSKGEVCSVEPLHAPPEARDHPITHYSLLAMASLTKILVIGLKPSLKVWITFPYSKSDPSSVPQLAWQFVPVQRTVNPVLAFSKGDTVHFLLVKKEETGTIHVIKQRQLHVSCDIISLCWINSRTLVLVDSHEKLQVVDRPSQEVLETLDLEQALVGEKACYQSVSSYAGQIIYLGTKSAHIMTLRAWRERIDCLLKLEHFPEALSLAWSFHEGTAKAVVGLFGDPVKRKGVVADKMVEMIFQFADFALKKCPEQGKIQVLEQHFHEVVPVLVDYCLLLKRLDLLFNQLYPQLVENMVAKGIFLESLESYIIADRLGHLPTPVMKDLLMHYQSSCMMESLERCIVHLDITSLDIQQVVQVCWENQLYDAVIYVFNRGMNDYTTPMEKLFAVIVPPVREGKSLTDVEVVMGNKLLVYISCCLAGRAYPLGDIPEDLVVQVKHQVFEFLIRRHSADSSEEEEVFPFIRTLLHFDTREFLNVLAMTFEDFKNDKQALEYQQRIVDILLQVMVDNPDFTPSQVGGLFTFLARQLAKPDNTLFVNRKLFDQVLEFLCCPDDDSRHTERQQVLLELLQVGSVVQFNEERLLMLAEKAKFYQICEFLYEKQHLYDRIIDCYLRDPLRKGEVFSYIHNLLSMPGYSPEEKQSVMDKVRQHMHELVIFDPNKSADLVTFHFAEEVQQIISELQDDRLLFRFLSCLLEQQDGQHSGPVLLLQPELHELQLDLLSRFDPKHILNFLQTSQHYRLEEAVQITQKYHHNKATAFLLEKRGDVEGAFAVLLETLKENLSRVTAERAGDDEEETLTNVKDSLSDIISLCHQSSQSLNQQQREVLWFPLLETMMAAQKQVKGRDSKHTFEVLKELTMKVLNRMSSFISLPAIIQRILQDPVYGKGKLAEIQSLILGMLDTFNYEQTLLETTTSLLNHDLHWSLAHLRAAVTRGIHPRQDCCNICLQQYKRRQDAAAADEIIVFSCGHLYHLQCLQQKDSGWGFTSDVQQQWSCYKCSSSKGGRAASADSRGRSLSLAQTCVTSLQHDGATEADGKKGFVEVTLDHQQEQSWDQLRCLYRGPSRLSILSELSHSNEKTGLLIGSQTGTDHFQLKLAPPPLFEE

Target ORF information:

RefSeq Version	XM_029835099.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes VPS8 subunit of CORVET complex (LOC101078974), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029835099.1

ORF Insert Sequence:

ATGTCAGATT CTCCAGACGC CAGAAGCTTG CTGTCTGCCT CCTCGCAGCT TAATTTGATG 
GAGGTGGACG CGATTGATGA TAAAGAGTTT GATATTCCCC AAGTGGACAC ACCTCCTACA 
CTGGAGAGTA TTCTTAATGA GCTGGAAGAT GAAGAGGAGC CATTTGTTCT GGAGGACACG 
TGTGTGCTGA ACACTGATAA CATGGATGCT CACTCCTGTG ACACTTCCTC TCTGGCCAGT 
TCGGACAGTG GAGATCCTGT ACACCACAAA AGGAGGAGGA GAACAGTGGA CATGAAACCA 
ACACTTCATG GCTCTGTTCT GCGCCATAGC TTGCTAAAAG GCATCTCTGC ACAGATCATC 
TCTGCTGCTG ACAAAGTGGA TGCCGGACTC CCAACTGCTA TAACAATGTC AGGTGTGATT 
GTTGTGGGGA CGTCTCACGG TCTGGCTCTG GTCTTTGGAA AAGATACCAA CCAGGCTCTG 
CGCCTCTGCC TGGGGAACAA AGCAACCGGG GCAGATTACG GAGCTGTATC TGCCCTCAGC 
ATCAACCATG ACTGCTCACG TCTGCTCTGT GGGTTTGCCA AGGGACAGAT TACAATGTGG 
GATCTCGCCA ATGGAAAACT CCTGAGAACT ATTACTGATG CACACCCCCC AGGGACGGCT 
ATACTTCACG TGAAGTTTAC AGACGATCCA ACTCTTGCAG TTTGCAATGA CAGTGGAGGA 
TCCGTGTTTG AGCTGGCGTT CAGGAGAGTG ATGGGGATGC GCTCCTGTGA CTCTCGATGT 
CTCTTCAGCG GTTCCAAAGG GGAGGTGTGC TCTGTTGAAC CCCTGCATGC TCCTCCAGAG 
GCCAGAGATC ACCCCATCAC CCACTATTCT CTCCTGGCCA TGGCCTCTCT CACTAAGATT 
CTGGTTATTG GCCTGAAGCC CTCACTTAAA GTCTGGATAA CGTTCCCCTA CAGTAAATCT 
GATCCGTCCA GTGTTCCCCA GCTGGCCTGG CAGTTTGTTC CAGTCCAGAG AACCGTGAAC 
CCCGTCCTGG CCTTTTCCAA AGGAGATACA GTTCACTTCC TCTTGGTAAA AAAGGAGGAA 
ACGGGCACCA TCCATGTAAT CAAACAAAGG CAGCTCCATG TCAGCTGTGA CATCATCAGC 
CTCTGTTGGA TCAACAGCCG CACGCTGGTC TTGGTGGACA GCCATGAAAA ACTGCAGGTT 
GTGGACCGAC CCAGTCAGGA GGTTTTGGAG ACCCTCGACT TGGAACAGGC TCTCGTTGGA 
GAAAAGGCCT GTTACCAGTC GGTCTCCAGC TACGCGGGAC AGATTATATA TCTGGGCACC 
AAGTCTGCTC ACATCATGAC TCTCAGGGCC TGGAGGGAGC GGATCGACTG TCTGCTGAAG 
CTGGAACATT TTCCCGAGGC CCTGTCTCTG GCCTGGTCGT TCCACGAGGG GACAGCTAAG 
GCTGTGGTCG GTCTGTTTGG AGACCCGGTG AAAAGAAAAG GAGTCGTTGC TGACAAGATG 
GTAGAGATGA TTTTCCAGTT TGCAGACTTT GCCCTAAAGA AGTGTCCAGA ACAAGGAAAG 
ATCCAAGTGC TGGAACAACA CTTCCATGAA GTTGTTCCAG TCCTGGTTGA TTACTGTTTG 
CTGCTGAAGA GGCTTGACCT GCTGTTTAAC CAGCTGTACC CTCAACTGGT GGAGAACATG 
GTGGCTAAAG GCATCTTCTT GGAATCCCTG GAGTCCTACA TTATCGCAGA CCGCCTTGGT 
CACCTTCCCA CACCGGTCAT GAAAGACCTT CTCATGCATT ACCAAAGCAG CTGTATGATG 
GAGAGCCTGG AGAGATGCAT CGTCCATCTG GACATCACCA GCCTGGACAT ACAGCAGGTG 
GTTCAGGTGT GTTGGGAGAA CCAACTCTAC GATGCGGTGA TCTACGTTTT CAATCGTGGC 
ATGAACGACT ACACTACACC TATGGAAAAA CTCTTTGCAG TAATTGTCCC ACCAGTAAGG 
GAGGGGAAGA GTCTTACAGA TGTGGAAGTG GTGATGGGAA ACAAACTCCT GGTGTATATC 
AGCTGCTGTC TGGCAGGACG GGCATATCCA TTAGGAGACA TCCCTGAAGA CCTTGTGGTC 
CAGGTAAAGC ATCAGGTGTT TGAATTTTTG ATCCGTCGCC ATTCAGCGGA CTCCTCAGAG 
GAGGAAGAAG TGTTCCCGTT TATTCGCACT CTGCTCCACT TTGACACTCG AGAGTTCCTC 
AACGTCCTCG CCATGACATT CGAAGACTTC AAGAACGACA AACAGGCCCT TGAATACCAG 
CAGAGGATCG TGGACATCTT ACTGCAGGTG ATGGTGGACA ACCCAGACTT TACTCCGTCC 
CAGGTGGGAG GACTCTTCAC CTTTTTAGCG CGACAGTTGG CCAAACCAGA CAACACACTT 
TTCGTAAACA GGAAGCTCTT CGATCAGGTC CTGGAGTTTC TGTGCTGCCC AGATGACGAC 
TCCCGTCACA CTGAGAGGCA GCAGGTTCTG TTGGAGCTGC TGCAGGTTGG GAGCGTGGTC 
CAGTTTAATG AAGAGAGGCT TCTGATGCTG GCCGAGAAAG CCAAATTCTA CCAGATATGT 
GAATTTCTTT ATGAGAAGCA ACATCTCTAC GACAGGATCA TTGACTGTTA CCTGAGAGAC 
CCCCTCAGAA AGGGAGAGGT CTTCAGCTAC ATCCACAATC TGCTGTCCAT GCCTGGTTAC 
AGCCCGGAGG AGAAGCAGTC AGTCATGGAT AAAGTTCGGC AGCATATGCA TGAGCTGGTG 
ATCTTTGACC CCAATAAATC AGCTGATTTA GTGACGTTTC ACTTTGCTGA AGAGGTCCAG 
CAAATAATCT CTGAGCTTCA GGATGATCGG CTGCTCTTCA GGTTCCTCAG CTGTCTCTTG 
GAGCAACAGG ACGGCCAACA TTCGGGCCCC GTCCTACTGC TGCAACCTGA ACTCCACGAG 
CTTCAGCTGG ACCTGCTCAG CCGTTTTGAC CCCAAACACA TTTTGAATTT CCTCCAGACC 
TCCCAACACT ATAGACTAGA AGAGGCAGTA CAGATAACAC AGAAGTATCA CCACAACAAA 
GCTACAGCCT TTCTTTTGGA GAAGAGGGGC GATGTCGAAG GAGCTTTTGC TGTGCTGTTG 
GAGACGCTAA AGGAAAACCT GAGTCGTGTC ACAGCTGAGA GAGCAGGAGA TGATGAAGAG 
GAAACCCTGA CAAATGTCAA GGATTCTCTA AGTGACATCA TCTCCTTGTG TCACCAGAGC 
TCTCAGAGCC TTAACCAGCA GCAAAGAGAG GTCCTGTGGT TTCCACTGTT GGAGACCATG 
ATGGCTGCAC AAAAACAAGT GAAGGGACGG GACAGTAAAC ACACATTTGA GGTGCTGAAG 
GAGCTGACGA TGAAGGTCCT CAACCGTATG AGCAGCTTCA TTTCTCTTCC CGCCATCATC 
CAGCGGATAC TCCAGGATCC AGTCTACGGG AAAGGAAAGC TGGCAGAAAT CCAGAGCCTT 
ATTTTGGGCA TGCTCGACAC TTTCAACTAT GAGCAGACGT TACTTGAAAC AACCACCAGT 
TTGTTGAACC ATGACCTCCA CTGGTCCCTG GCGCACTTGC GCGCAGCGGT CACTAGGGGG 
ATCCACCCAC GACAGGACTG CTGCAACATC TGCCTGCAGC AGTACAAGAG GAGGCAGGAT 
GCAGCAGCAG CAGACGAGAT CATCGTCTTC AGCTGTGGGC ATCTGTACCA CCTCCAGTGT 
CTGCAGCAGA AGGACTCTGG CTGGGGTTTT ACGTCAGATG TTCAGCAGCA ATGGAGCTGC 
TACAAATGTT CCTCCAGCAA AGGAGGCCGA GCGGCGTCGG CTGATTCGAG AGGCCGCAGC 
TTGTCCCTGG CGCAGACCTG CGTTACATCA TTACAACACG ATGGGGCAAC AGAAGCTGAC 
GGGAAGAAGG GGTTTGTCGA AGTCACCTTA GACCATCAGC AAGAACAGTC CTGGGATCAG 
CTACGCTGCC TTTACAGAGG ACCGTCCAGG CTGTCCATCC TGTCCGAACT TTCACACAGT 
AACGAGAAGA CGGGCCTTCT GATCGGCAGC CAGACGGGGA CAGATCACTT CCAGCTGAAA 
CTGGCCCCCC CGCCTCTGTT CGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101078974 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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