lats2 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	lats2
Entrez Gene ID	101072449
Full Name	large tumor suppressor kinase 2
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003962165.3	XP_003962214.1	serine/threonine-protein kinase LATS2
XM_011613500.1	XP_011611802.1	serine/threonine-protein kinase LATS2 isoform X2
XM_003962165.2	XP_003962214.1	serine/threonine-protein kinase LATS2 isoform X1

Pathways from BioSystems
Gene Ontology

The following lats2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lats2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo01203	XM_003962165.2 Latest version!	Takifugu rubripes large tumor suppressor kinase 2 (lats2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo26863	XM_011613500.1 Latest version!	Takifugu rubripes large tumor suppressor kinase 2 (lats2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo42617	XM_003962165.3 Latest version!	Takifugu rubripes large tumor suppressor kinase 2 (lats2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lats2 ( XM_003962165.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003962214.1 Takifugu rubripes large tumor suppressor kinase 2 (lats2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo01203
Clone ID Related Accession (Same CDS sequence)	XM_003962165.2
Accession Version	XM_003962165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	serine/threonine-protein kinase LATS2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070914.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003962165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCCA AGACTTTCCC TGCTGCCCCG TATGTGGGCA ACACGCGCCA GAGGCTCCAG 
GAGATCAAGG AGGGTCTGAA GCAGCCAGCC AAGCTAGTGA GCCAGGCCCT GCATGGGGGC 
AGCTCTCGGG CGGAGGGCAG CAGAGGGGCT GACTGCAAAA GCGGGAAAGA CGCAGCCAGC 
CGACAGCAGC AGCTCCGTCC ACCGCAGAAG TTCAACAACT ACCAGAATGC CCTGAGAGAA 
ATTCGGAGGT CCCTCATGCC CTTTGCCAAC GAGTCCGGCC CGTCCTCTGG ATCGGGACAT 
CCTGCGGGGG CCGGGGAGGT GAACCGGCAG ATGCTCCAAG AGCTGGTCAA CGCTGGCTGC 
GACCAGGAGA TGGCAGTGCG GGCACTGAAG CAGACGGGCA GCCGGAACAT TGAAGCAGCC 
CTGGAGTACA TCAGTAAAAT GGGCTACCTT GATCCTCGCA ACGAGCTCAT CGTCCGCGTC 
ATCAAGCAGA CGTCACCAGG AAAAGCTGGC ATTCCAAACT CGATGGACCA CCGACCTTCC 
CTAGAAGCCT CAGGTGAAGC AGGGGCCATG CCTCCATACC ACCAGATGGG GGCTCCTATG 
TATGAGGGGC CAGCGGGATA CACCCCCGAT GGTGAGATCT CCCGACCTTA CATGAGCTCT 
GCCCCTGTGA TGAACTACAT GATGCCACCG ACAAGTTCAG CACAAGGACC GGCCATGGGA 
AACCCCATGG GTCGACCCCC GACTCTAGGC ACCTATCCAG CGGTGATGGG CAGCCAGGGC 
AACCCGGCCA ATACCATGTA CCCTCCAGGA GCCCAGCAGA AGAATTATCC AGGTAGCATG 
GAGCAGCATG GGCCCATGAT GAGCTACAAT GTTCCTGGGC AGCCTCTGCA GCTCCAACCT 
CAGCCTCCTG GGGGCCCTGT CCCAGGCCCA CATTATGACT ACAGCCATGC CAGGCCACAC 
ATGATGGATC CTGCAGGCTA TGGAGTGAAG CGGACCGCCT CCTTTCAGAA CAAGATGGCA 
CCGCCTCCCA TGGCACCCCC AGACAACTAC GTTAACATGC AAGGGAAAGG TGCCATGGGT 
CAGAATGTTC CAGGGGGAGG AGGAGCTGGA GGGGGTGGAG GGTATCCTGC CAACCGGTAC 
CTGCCCCCAC ATTCCCACCC ACGCCAGGCC AGCCCCACCT CCCACCAGGT TCACCTGATG 
TCCCGATCCC CGGGAGGGGT AGCAGCCATG GGCCCTGACT TCTCTGACAT ACCCCAAGGC 
TTGATGACGC CGTCCAGGGC CAGTCTTAAC CTGGACATTT ATGAACACCA CTGGGCTGGA 
CCTCCGGGCC CTGAAGGGGC CCCGCCAGGT AGGCAACCCC AACCACAAGG CCCATTCAGG 
GGTGAAGTTC GTGTCCCGAG CCGAACCAAC TCCTTCAACA GCCGTTCTGC TGGGCCCAAT 
GGCGTCCGAC CAACTCTGAC GGCACCCCCG ACAGCTGGGA AACAAGATCC TTCTCTGGGC 
CCACCTAACA CAATCACCGC TGTGACGTCC CCCCCAATCC AGCAGCCAGT CAAGAGCATC 
CGTGTAATGA GGCCAGAGCC CAAGACGGCT GTGGGACCAT GTCACCCTGG CTGGATGGCC 
GCTCAGACCC AGGATGCCGG CGAGCCTCTG GCTTATATGG CAGAGGAACC GTACCCCATT 
GAGCCGGCTC AGGAGCCACG CTGCCCACCA CCCCCTTATC CCAAAAACCT GCTCATGCCC 
GGCCCAACTG CAGAACCGGG GGGCTTGGAA GCAGCCGGAG CCATGTGTGG AGCTCAGGAC 
ATCGGCAACG CCGCCGTCAG AAGTACACAC GGCAGCGGCA GTGGAGGGAG CGGGAAGGTG 
GAGGAGAGCC ATCAGGTGAA AGAGAAGACG AAGATGTGCA AGACGGACAA AACCGATAAA 
ACTGTAAAAG ACAAGAAGCA AATCCAGACG TCACCCGTCC CGGTCAGAAA GAATGGGCGC 
GACGAGGAGA AGAGGGAGTC GCGCATCAAG ACTTACTCGC CTTTTGCCTT CAAGTTCTAC 
ATGGAGCAGC ACATCGAGAA CGTGATGAAG ACCTACAACC AGAAACTGAA CCGCAGGCTT 
CAGCTGGAGC AAGAGATGTC CAAGGCCGGC CTTTCGGAAG CGGAACAAGA ACAGATGAGG 
AAGATGCTGA ACCAGAAAGA GTCCAATTAT AACAGGCTGC GCCGTGCCAA AATGGACAAG 
TCTATGTTTG TGAAAATCAA GACGTTGGGC ATAGGAGCCT TTGGGGAAGT GTGCCTCACA 
CGTAAGGTGG ACACCTGTGC GCTTTATGCC ATGAAAACAC TGCGGAAGAA AGACGTCCTC 
AACCGCAATC AGGTGGCCCA CGTGAAAGCA GAACGCGACA TCCTGGCTGA GGCAGACAAC 
GAGTGGGTGG TGCGTCTCTA CTACTCCTTC CAGGATCGAG AGAGCCTCTA CTTTGTCATG 
GACTACATAC CCGGGGGAGA TATGATGAGC CTGCTCATCC GAATGGGGGT CTTCCCTGAG 
ATCCTGGCGT GTTTCTACGT GGCGGAGCTG ACGCTGGCCA TCGAGAGCGT CCACAAGATG 
GGCTTTATCC ACCGCGACAT CAAGCCCGAC AACATCCTCA TTGATCTGGA CGGACACATA 
AAGCTGACGG ACTTTGGCCT ATGTACGGGA TTCCGCTGGA CGCACAACTC CAAGTATTAC 
CAGAAAGGGA GCCACGTGAG GCAGGACAGC ATGGAGCCCA GCGACTTTTG GGACGACGTT 
TCCAACTGTC GCTGTGGCGA CCGGCTGATG ACGCTAGAGC AGCGCGCCAA TCGTCAGCAT 
CAGCGCTGCC TGGCTCATTC GCTGGTCGGA ACGCCAAACT ACATCGCACC AGAGGTGCTG 
TTGCGTAAAG GTTACACCCA GCTGTGTGAC TGGTGGAGTG TGGGAGTGAT CCTCTTCGAG 
ATGCTGGTGG GACAGCCGCC CTTCCTGGCT CCCACACCCA CTGAGACTCA GATTAAGGTC 
ATTAATTGGG AGAGTACCCT GCAGGTGCCC GCCCAAGTCA AACTCAGTCC AGAGGCCATC 
GACATCATCG GCCGCCTCTG CTGCTCTGCA GAAGATCGTC TGGGTGCCAA CGGGGCTGGC 
GAGATAAAAG CCCACCCCTT TTTCTCCCAG ACTGACTTCT CCAGCAACCT GCGGCAGCAG 
CCGGCCCCCT ACCGCCCCAA GATCGCCCAT CCGATGGACA CGTCCAACTT CGACCCCGTG 
GAGGAGGAAG GTGGCCCCGG AGCGTGGAGC GACAGTGGGG ACAGCACCCG GACTTTGGAC 
ATGCTTTGCT CCCCTCAGGG GAAGCACCCA GAGCACGCCT TCTACGAGTT CACCTTCCGC 
AGATTTTTTG ACGACAACGG CTGCCCCTTC CGCTACCCGA AGCCCCCGGA AGCCTGTGAG 
GCGTCGCCGC ATATGACAGG AGCCGGCTGC TTGGGCCCAG AGGAGGAGGA CGAGCAAGAA 
GAACAGGACA CGGAAGATGA AGAGGAGGAT GGAGAGCAGG GGGAGGGATG CGAGCCAGTC 
TATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003962214.1
CDS	589..4137
Translation	MRPKTFPAAPYVGNTRQRLQEIKEGLKQPAKLVSQALHGGSSRAEGSRGADCKSGKDAASRQQQLRPPQKFNNYQNALREIRRSLMPFANESGPSSGSGHPAGAGEVNRQMLQELVNAGCDQEMAVRALKQTGSRNIEAALEYISKMGYLDPRNELIVRVIKQTSPGKAGIPNSMDHRPSLEASGEAGAMPPYHQMGAPMYEGPAGYTPDGEISRPYMSSAPVMNYMMPPTSSAQGPAMGNPMGRPPTLGTYPAVMGSQGNPANTMYPPGAQQKNYPGSMEQHGPMMSYNVPGQPLQLQPQPPGGPVPGPHYDYSHARPHMMDPAGYGVKRTASFQNKMAPPPMAPPDNYVNMQGKGAMGQNVPGGGGAGGGGGYPANRYLPPHSHPRQASPTSHQVHLMSRSPGGVAAMGPDFSDIPQGLMTPSRASLNLDIYEHHWAGPPGPEGAPPGRQPQPQGPFRGEVRVPSRTNSFNSRSAGPNGVRPTLTAPPTAGKQDPSLGPPNTITAVTSPPIQQPVKSIRVMRPEPKTAVGPCHPGWMAAQTQDAGEPLAYMAEEPYPIEPAQEPRCPPPPYPKNLLMPGPTAEPGGLEAAGAMCGAQDIGNAAVRSTHGSGSGGSGKVEESHQVKEKTKMCKTDKTDKTVKDKKQIQTSPVPVRKNGRDEEKRESRIKTYSPFAFKFYMEQHIENVMKTYNQKLNRRLQLEQEMSKAGLSEAEQEQMRKMLNQKESNYNRLRRAKMDKSMFVKIKTLGIGAFGEVCLTRKVDTCALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRESLYFVMDYIPGGDMMSLLIRMGVFPEILACFYVAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSDFWDDVSNCRCGDRLMTLEQRANRQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQIKVINWESTLQVPAQVKLSPEAIDIIGRLCCSAEDRLGANGAGEIKAHPFFSQTDFSSNLRQQPAPYRPKIAHPMDTSNFDPVEEEGGPGAWSDSGDSTRTLDMLCSPQGKHPEHAFYEFTFRRFFDDNGCPFRYPKPPEACEASPHMTGAGCLGPEEEDEQEEQDTEDEEEDGEQGEGCEPVYV

Target ORF information:

RefSeq Version	XM_003962165.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes large tumor suppressor kinase 2 (lats2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003962165.2

ORF Insert Sequence:

ATGAGGCCCA AGACTTTCCC TGCTGCCCCG TATGTGGGCA ACACGCGCCA GAGGCTCCAG 
GAGATCAAGG AGGGTCTGAA GCAGCCAGCC AAGCTAGTGA GCCAGGCCCT GCATGGGGGC 
AGCTCTCGGG CGGAGGGCAG CAGAGGGGCT GACTGCAAAA GCGGGAAAGA CGCAGCCAGC 
CGACAGCAGC AGCTCCGTCC ACCGCAGAAG TTCAACAACT ACCAGAATGC CCTGAGAGAA 
ATTCGGAGGT CCCTCATGCC CTTTGCCAAC GAGTCCGGCC CGTCCTCTGG ATCGGGACAT 
CCTGCGGGGG CCGGGGAGGT GAACCGGCAG ATGCTCCAAG AGCTGGTCAA CGCTGGCTGC 
GACCAGGAGA TGGCAGTGCG GGCACTGAAG CAGACGGGCA GCCGGAACAT TGAAGCAGCC 
CTGGAGTACA TCAGTAAAAT GGGCTACCTT GATCCTCGCA ACGAGCTCAT CGTCCGCGTC 
ATCAAGCAGA CGTCACCAGG AAAAGCTGGC ATTCCAAACT CGATGGACCA CCGACCTTCC 
CTAGAAGCCT CAGGTGAAGC AGGGGCCATG CCTCCATACC ACCAGATGGG GGCTCCTATG 
TATGAGGGGC CAGCGGGATA CACCCCCGAT GGTGAGATCT CCCGACCTTA CATGAGCTCT 
GCCCCTGTGA TGAACTACAT GATGCCACCG ACAAGTTCAG CACAAGGACC GGCCATGGGA 
AACCCCATGG GTCGACCCCC GACTCTAGGC ACCTATCCAG CGGTGATGGG CAGCCAGGGC 
AACCCGGCCA ATACCATGTA CCCTCCAGGA GCCCAGCAGA AGAATTATCC AGGTAGCATG 
GAGCAGCATG GGCCCATGAT GAGCTACAAT GTTCCTGGGC AGCCTCTGCA GCTCCAACCT 
CAGCCTCCTG GGGGCCCTGT CCCAGGCCCA CATTATGACT ACAGCCATGC CAGGCCACAC 
ATGATGGATC CTGCAGGCTA TGGAGTGAAG CGGACCGCCT CCTTTCAGAA CAAGATGGCA 
CCGCCTCCCA TGGCACCCCC AGACAACTAC GTTAACATGC AAGGGAAAGG TGCCATGGGT 
CAGAATGTTC CAGGGGGAGG AGGAGCTGGA GGGGGTGGAG GGTATCCTGC CAACCGGTAC 
CTGCCCCCAC ATTCCCACCC ACGCCAGGCC AGCCCCACCT CCCACCAGGT TCACCTGATG 
TCCCGATCCC CGGGAGGGGT AGCAGCCATG GGCCCTGACT TCTCTGACAT ACCCCAAGGC 
TTGATGACGC CGTCCAGGGC CAGTCTTAAC CTGGACATTT ATGAACACCA CTGGGCTGGA 
CCTCCGGGCC CTGAAGGGGC CCCGCCAGGT AGGCAACCCC AACCACAAGG CCCATTCAGG 
GGTGAAGTTC GTGTCCCGAG CCGAACCAAC TCCTTCAACA GCCGTTCTGC TGGGCCCAAT 
GGCGTCCGAC CAACTCTGAC GGCACCCCCG ACAGCTGGGA AACAAGATCC TTCTCTGGGC 
CCACCTAACA CAATCACCGC TGTGACGTCC CCCCCAATCC AGCAGCCAGT CAAGAGCATC 
CGTGTAATGA GGCCAGAGCC CAAGACGGCT GTGGGACCAT GTCACCCTGG CTGGATGGCC 
GCTCAGACCC AGGATGCCGG CGAGCCTCTG GCTTATATGG CAGAGGAACC GTACCCCATT 
GAGCCGGCTC AGGAGCCACG CTGCCCACCA CCCCCTTATC CCAAAAACCT GCTCATGCCC 
GGCCCAACTG CAGAACCGGG GGGCTTGGAA GCAGCCGGAG CCATGTGTGG AGCTCAGGAC 
ATCGGCAACG CCGCCGTCAG AAGTACACAC GGCAGCGGCA GTGGAGGGAG CGGGAAGGTG 
GAGGAGAGCC ATCAGGTGAA AGAGAAGACG AAGATGTGCA AGACGGACAA AACCGATAAA 
ACTGTAAAAG ACAAGAAGCA AATCCAGACG TCACCCGTCC CGGTCAGAAA GAATGGGCGC 
GACGAGGAGA AGAGGGAGTC GCGCATCAAG ACTTACTCGC CTTTTGCCTT CAAGTTCTAC 
ATGGAGCAGC ACATCGAGAA CGTGATGAAG ACCTACAACC AGAAACTGAA CCGCAGGCTT 
CAGCTGGAGC AAGAGATGTC CAAGGCCGGC CTTTCGGAAG CGGAACAAGA ACAGATGAGG 
AAGATGCTGA ACCAGAAAGA GTCCAATTAT AACAGGCTGC GCCGTGCCAA AATGGACAAG 
TCTATGTTTG TGAAAATCAA GACGTTGGGC ATAGGAGCCT TTGGGGAAGT GTGCCTCACA 
CGTAAGGTGG ACACCTGTGC GCTTTATGCC ATGAAAACAC TGCGGAAGAA AGACGTCCTC 
AACCGCAATC AGGTGGCCCA CGTGAAAGCA GAACGCGACA TCCTGGCTGA GGCAGACAAC 
GAGTGGGTGG TGCGTCTCTA CTACTCCTTC CAGGATCGAG AGAGCCTCTA CTTTGTCATG 
GACTACATAC CCGGGGGAGA TATGATGAGC CTGCTCATCC GAATGGGGGT CTTCCCTGAG 
ATCCTGGCGT GTTTCTACGT GGCGGAGCTG ACGCTGGCCA TCGAGAGCGT CCACAAGATG 
GGCTTTATCC ACCGCGACAT CAAGCCCGAC AACATCCTCA TTGATCTGGA CGGACACATA 
AAGCTGACGG ACTTTGGCCT ATGTACGGGA TTCCGCTGGA CGCACAACTC CAAGTATTAC 
CAGAAAGGGA GCCACGTGAG GCAGGACAGC ATGGAGCCCA GCGACTTTTG GGACGACGTT 
TCCAACTGTC GCTGTGGCGA CCGGCTGATG ACGCTAGAGC AGCGCGCCAA TCGTCAGCAT 
CAGCGCTGCC TGGCTCATTC GCTGGTCGGA ACGCCAAACT ACATCGCACC AGAGGTGCTG 
TTGCGTAAAG GTTACACCCA GCTGTGTGAC TGGTGGAGTG TGGGAGTGAT CCTCTTCGAG 
ATGCTGGTGG GACAGCCGCC CTTCCTGGCT CCCACACCCA CTGAGACTCA GATTAAGGTC 
ATTAATTGGG AGAGTACCCT GCAGGTGCCC GCCCAAGTCA AACTCAGTCC AGAGGCCATC 
GACATCATCG GCCGCCTCTG CTGCTCTGCA GAAGATCGTC TGGGTGCCAA CGGGGCTGGC 
GAGATAAAAG CCCACCCCTT TTTCTCCCAG ACTGACTTCT CCAGCAACCT GCGGCAGCAG 
CCGGCCCCCT ACCGCCCCAA GATCGCCCAT CCGATGGACA CGTCCAACTT CGACCCCGTG 
GAGGAGGAAG GTGGCCCCGG AGCGTGGAGC GACAGTGGGG ACAGCACCCG GACTTTGGAC 
ATGCTTTGCT CCCCTCAGGG GAAGCACCCA GAGCACGCCT TCTACGAGTT CACCTTCCGC 
AGATTTTTTG ACGACAACGG CTGCCCCTTC CGCTACCCGA AGCCCCCGGA AGCCTGTGAG 
GCGTCGCCGC ATATGACAGG AGCCGGCTGC TTGGGCCCAG AGGAGGAGGA CGAGCAAGAA 
GAACAGGACA CGGAAGATGA AGAGGAGGAT GGAGAGCAGG GGGAGGGATG CGAGCCAGTC 
TATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats2 ( XM_011613500.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011611802.1 Takifugu rubripes large tumor suppressor kinase 2 (lats2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo26863
Clone ID Related Accession (Same CDS sequence)	XM_011613500.1
Accession Version	XM_011613500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	serine/threonine-protein kinase LATS2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070914.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCCA AGACTTTCCC TGCTGCCCCG TATGTGGGCA ACACGCGCCA GAGGCTCCAG 
GAGATCAAGG AGGGTCTGAA GCAGCCAGCC AAGCTAGTGA GCCAGGCCCT GCATGGGGGC 
AGCTCTCGGG CGGAGGGCAG CAGAGGGGCT GACTGCAAAA GCGGGAAAGA CGCAGCCAGC 
CGACAGCAGC AGCTCCGTCC ACCGCAGAAG TTCAACAACT ACCAGAATGC CCTGAGAGAA 
ATTCGGAGGT CCCTCATGCC CTTTGCCAAC GAGTCCGGCC CGTCCTCTGG ATCGGGACAT 
CCTGCGGGGG CCGGGGAGGT GAACCGGCAG ATGCTCCAAG AGCTGGTCAA CGCTGGCTGC 
GACCAGATGG CAGTGCGGGC ACTGAAGCAG ACGGGCAGCC GGAACATTGA AGCAGCCCTG 
GAGTACATCA GTAAAATGGG CTACCTTGAT CCTCGCAACG AGCTCATCGT CCGCGTCATC 
AAGCAGACGT CACCAGGAAA AGCTGGCATT CCAAACTCGA TGGACCACCG ACCTTCCCTA 
GAAGCCTCAG GTGAAGCAGG GGCCATGCCT CCATACCACC AGATGGGGGC TCCTATGTAT 
GAGGGGCCAG CGGGATACAC CCCCGATGGT GAGATCTCCC GACCTTACAT GAGCTCTGCC 
CCTGTGATGA ACTACATGAT GCCACCGACA AGTTCAGCAC AAGGACCGGC CATGGGAAAC 
CCCATGGGTC GACCCCCGAC TCTAGGCACC TATCCAGCGG TGATGGGCAG CCAGGGCAAC 
CCGGCCAATA CCATGTACCC TCCAGGAGCC CAGCAGAAGA ATTATCCAGG TAGCATGGAG 
CAGCATGGGC CCATGATGAG CTACAATGTT CCTGGGCAGC CTCTGCAGCT CCAACCTCAG 
CCTCCTGGGG GCCCTGTCCC AGGCCCACAT TATGACTACA GCCATGCCAG GCCACACATG 
ATGGATCCTG CAGGCTATGG AGTGAAGCGG ACCGCCTCCT TTCAGAACAA GATGGCACCG 
CCTCCCATGG CACCCCCAGA CAACTACGTT AACATGCAAG GGAAAGGTGC CATGGGTCAG 
AATGTTCCAG GGGGAGGAGG AGCTGGAGGG GGTGGAGGGT ATCCTGCCAA CCGGTACCTG 
CCCCCACATT CCCACCCACG CCAGGCCAGC CCCACCTCCC ACCAGGTTCA CCTGATGTCC 
CGATCCCCGG GAGGGGTAGC AGCCATGGGC CCTGACTTCT CTGACATACC CCAAGGCTTG 
ATGACGCCGT CCAGGGCCAG TCTTAACCTG GACATTTATG AACACCACTG GGCTGGACCT 
CCGGGCCCTG AAGGGGCCCC GCCAGGTAGG CAACCCCAAC CACAAGGCCC ATTCAGGGGT 
GAAGTTCGTG TCCCGAGCCG AACCAACTCC TTCAACAGCC GTTCTGCTGG GCCCAATGGC 
GTCCGACCAA CTCTGACGGC ACCCCCGACA GCTGGGAAAC AAGATCCTTC TCTGGGCCCA 
CCTAACACAA TCACCGCTGT GACGTCCCCC CCAATCCAGC AGCCAGTCAA GAGCATCCGT 
GTAATGAGGC CAGAGCCCAA GACGGCTGTG GGACCATGTC ACCCTGGCTG GATGGCCGCT 
CAGACCCAGG ATGCCGGCGA GCCTCTGGCT TATATGGCAG AGGAACCGTA CCCCATTGAG 
CCGGCTCAGG AGCCACGCTG CCCACCACCC CCTTATCCCA AAAACCTGCT CATGCCCGGC 
CCAACTGCAG AACCGGGGGG CTTGGAAGCA GCCGGAGCCA TGTGTGGAGC TCAGGACATC 
GGCAACGCCG CCGTCAGAAG TACACACGGC AGCGGCAGTG GAGGGAGCGG GAAGGTGGAG 
GAGAGCCATC AGGTGAAAGA GAAGACGAAG ATGTGCAAGA CGGACAAAAC CGATAAAACT 
GTAAAAGACA AGAAGCAAAT CCAGACGTCA CCCGTCCCGG TCAGAAAGAA TGGGCGCGAC 
GAGGAGAAGA GGGAGTCGCG CATCAAGACT TACTCGCCTT TTGCCTTCAA GTTCTACATG 
GAGCAGCACA TCGAGAACGT GATGAAGACC TACAACCAGA AACTGAACCG CAGGCTTCAG 
CTGGAGCAAG AGATGTCCAA GGCCGGCCTT TCGGAAGCGG AACAAGAACA GATGAGGAAG 
ATGCTGAACC AGAAAGAGTC CAATTATAAC AGGCTGCGCC GTGCCAAAAT GGACAAGTCT 
ATGTTTGTGA AAATCAAGAC GTTGGGCATA GGAGCCTTTG GGGAAGTGTG CCTCACACGT 
AAGGTGGACA CCTGTGCGCT TTATGCCATG AAAACACTGC GGAAGAAAGA CGTCCTCAAC 
CGCAATCAGG TGGCCCACGT GAAAGCAGAA CGCGACATCC TGGCTGAGGC AGACAACGAG 
TGGGTGGTGC GTCTCTACTA CTCCTTCCAG GATCGAGAGA GCCTCTACTT TGTCATGGAC 
TACATACCCG GGGGAGATAT GATGAGCCTG CTCATCCGAA TGGGGGTCTT CCCTGAGATC 
CTGGCGTGTT TCTACGTGGC GGAGCTGACG CTGGCCATCG AGAGCGTCCA CAAGATGGGC 
TTTATCCACC GCGACATCAA GCCCGACAAC ATCCTCATTG ATCTGGACGG ACACATAAAG 
CTGACGGACT TTGGCCTATG TACGGGATTC CGCTGGACGC ACAACTCCAA GTATTACCAG 
AAAGGGAGCC ACGTGAGGCA GGACAGCATG GAGCCCAGCG ACTTTTGGGA CGACGTTTCC 
AACTGTCGCT GTGGCGACCG GCTGATGACG CTAGAGCAGC GCGCCAATCG TCAGCATCAG 
CGCTGCCTGG CTCATTCGCT GGTCGGAACG CCAAACTACA TCGCACCAGA GGTGCTGTTG 
CGTAAAGGTT ACACCCAGCT GTGTGACTGG TGGAGTGTGG GAGTGATCCT CTTCGAGATG 
CTGGTGGGAC AGCCGCCCTT CCTGGCTCCC ACACCCACTG AGACTCAGAT TAAGGTCATT 
AATTGGGAGA GTACCCTGCA GGTGCCCGCC CAAGTCAAAC TCAGTCCAGA GGCCATCGAC 
ATCATCGGCC GCCTCTGCTG CTCTGCAGAA GATCGTCTGG GTGCCAACGG GGCTGGCGAG 
ATAAAAGCCC ACCCCTTTTT CTCCCAGACT GACTTCTCCA GCAACCTGCG GCAGCAGCCG 
GCCCCCTACC GCCCCAAGAT CGCCCATCCG ATGGACACGT CCAACTTCGA CCCCGTGGAG 
GAGGAAGGTG GCCCCGGAGC GTGGAGCGAC AGTGGGGACA GCACCCGGAC TTTGGACATG 
CTTTGCTCCC CTCAGGGGAA GCACCCAGAG CACGCCTTCT ACGAGTTCAC CTTCCGCAGA 
TTTTTTGACG ACAACGGCTG CCCCTTCCGC TACCCGAAGC CCCCGGAAGC CTGTGAGGCG 
TCGCCGCATA TGACAGGAGC CGGCTGCTTG GGCCCAGAGG AGGAGGACGA GCAAGAAGAA 
CAGGACACGG AAGATGAAGA GGAGGATGGA GAGCAGGGGG AGGGATGCGA GCCAGTCTAT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011611802.1
CDS	589..4134
Translation	MRPKTFPAAPYVGNTRQRLQEIKEGLKQPAKLVSQALHGGSSRAEGSRGADCKSGKDAASRQQQLRPPQKFNNYQNALREIRRSLMPFANESGPSSGSGHPAGAGEVNRQMLQELVNAGCDQMAVRALKQTGSRNIEAALEYISKMGYLDPRNELIVRVIKQTSPGKAGIPNSMDHRPSLEASGEAGAMPPYHQMGAPMYEGPAGYTPDGEISRPYMSSAPVMNYMMPPTSSAQGPAMGNPMGRPPTLGTYPAVMGSQGNPANTMYPPGAQQKNYPGSMEQHGPMMSYNVPGQPLQLQPQPPGGPVPGPHYDYSHARPHMMDPAGYGVKRTASFQNKMAPPPMAPPDNYVNMQGKGAMGQNVPGGGGAGGGGGYPANRYLPPHSHPRQASPTSHQVHLMSRSPGGVAAMGPDFSDIPQGLMTPSRASLNLDIYEHHWAGPPGPEGAPPGRQPQPQGPFRGEVRVPSRTNSFNSRSAGPNGVRPTLTAPPTAGKQDPSLGPPNTITAVTSPPIQQPVKSIRVMRPEPKTAVGPCHPGWMAAQTQDAGEPLAYMAEEPYPIEPAQEPRCPPPPYPKNLLMPGPTAEPGGLEAAGAMCGAQDIGNAAVRSTHGSGSGGSGKVEESHQVKEKTKMCKTDKTDKTVKDKKQIQTSPVPVRKNGRDEEKRESRIKTYSPFAFKFYMEQHIENVMKTYNQKLNRRLQLEQEMSKAGLSEAEQEQMRKMLNQKESNYNRLRRAKMDKSMFVKIKTLGIGAFGEVCLTRKVDTCALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRESLYFVMDYIPGGDMMSLLIRMGVFPEILACFYVAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSDFWDDVSNCRCGDRLMTLEQRANRQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQIKVINWESTLQVPAQVKLSPEAIDIIGRLCCSAEDRLGANGAGEIKAHPFFSQTDFSSNLRQQPAPYRPKIAHPMDTSNFDPVEEEGGPGAWSDSGDSTRTLDMLCSPQGKHPEHAFYEFTFRRFFDDNGCPFRYPKPPEACEASPHMTGAGCLGPEEEDEQEEQDTEDEEEDGEQGEGCEPVYV

Target ORF information:

RefSeq Version	XM_011613500.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes large tumor suppressor kinase 2 (lats2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011613500.1

ORF Insert Sequence:

ATGAGGCCCA AGACTTTCCC TGCTGCCCCG TATGTGGGCA ACACGCGCCA GAGGCTCCAG 
GAGATCAAGG AGGGTCTGAA GCAGCCAGCC AAGCTAGTGA GCCAGGCCCT GCATGGGGGC 
AGCTCTCGGG CGGAGGGCAG CAGAGGGGCT GACTGCAAAA GCGGGAAAGA CGCAGCCAGC 
CGACAGCAGC AGCTCCGTCC ACCGCAGAAG TTCAACAACT ACCAGAATGC CCTGAGAGAA 
ATTCGGAGGT CCCTCATGCC CTTTGCCAAC GAGTCCGGCC CGTCCTCTGG ATCGGGACAT 
CCTGCGGGGG CCGGGGAGGT GAACCGGCAG ATGCTCCAAG AGCTGGTCAA CGCTGGCTGC 
GACCAGATGG CAGTGCGGGC ACTGAAGCAG ACGGGCAGCC GGAACATTGA AGCAGCCCTG 
GAGTACATCA GTAAAATGGG CTACCTTGAT CCTCGCAACG AGCTCATCGT CCGCGTCATC 
AAGCAGACGT CACCAGGAAA AGCTGGCATT CCAAACTCGA TGGACCACCG ACCTTCCCTA 
GAAGCCTCAG GTGAAGCAGG GGCCATGCCT CCATACCACC AGATGGGGGC TCCTATGTAT 
GAGGGGCCAG CGGGATACAC CCCCGATGGT GAGATCTCCC GACCTTACAT GAGCTCTGCC 
CCTGTGATGA ACTACATGAT GCCACCGACA AGTTCAGCAC AAGGACCGGC CATGGGAAAC 
CCCATGGGTC GACCCCCGAC TCTAGGCACC TATCCAGCGG TGATGGGCAG CCAGGGCAAC 
CCGGCCAATA CCATGTACCC TCCAGGAGCC CAGCAGAAGA ATTATCCAGG TAGCATGGAG 
CAGCATGGGC CCATGATGAG CTACAATGTT CCTGGGCAGC CTCTGCAGCT CCAACCTCAG 
CCTCCTGGGG GCCCTGTCCC AGGCCCACAT TATGACTACA GCCATGCCAG GCCACACATG 
ATGGATCCTG CAGGCTATGG AGTGAAGCGG ACCGCCTCCT TTCAGAACAA GATGGCACCG 
CCTCCCATGG CACCCCCAGA CAACTACGTT AACATGCAAG GGAAAGGTGC CATGGGTCAG 
AATGTTCCAG GGGGAGGAGG AGCTGGAGGG GGTGGAGGGT ATCCTGCCAA CCGGTACCTG 
CCCCCACATT CCCACCCACG CCAGGCCAGC CCCACCTCCC ACCAGGTTCA CCTGATGTCC 
CGATCCCCGG GAGGGGTAGC AGCCATGGGC CCTGACTTCT CTGACATACC CCAAGGCTTG 
ATGACGCCGT CCAGGGCCAG TCTTAACCTG GACATTTATG AACACCACTG GGCTGGACCT 
CCGGGCCCTG AAGGGGCCCC GCCAGGTAGG CAACCCCAAC CACAAGGCCC ATTCAGGGGT 
GAAGTTCGTG TCCCGAGCCG AACCAACTCC TTCAACAGCC GTTCTGCTGG GCCCAATGGC 
GTCCGACCAA CTCTGACGGC ACCCCCGACA GCTGGGAAAC AAGATCCTTC TCTGGGCCCA 
CCTAACACAA TCACCGCTGT GACGTCCCCC CCAATCCAGC AGCCAGTCAA GAGCATCCGT 
GTAATGAGGC CAGAGCCCAA GACGGCTGTG GGACCATGTC ACCCTGGCTG GATGGCCGCT 
CAGACCCAGG ATGCCGGCGA GCCTCTGGCT TATATGGCAG AGGAACCGTA CCCCATTGAG 
CCGGCTCAGG AGCCACGCTG CCCACCACCC CCTTATCCCA AAAACCTGCT CATGCCCGGC 
CCAACTGCAG AACCGGGGGG CTTGGAAGCA GCCGGAGCCA TGTGTGGAGC TCAGGACATC 
GGCAACGCCG CCGTCAGAAG TACACACGGC AGCGGCAGTG GAGGGAGCGG GAAGGTGGAG 
GAGAGCCATC AGGTGAAAGA GAAGACGAAG ATGTGCAAGA CGGACAAAAC CGATAAAACT 
GTAAAAGACA AGAAGCAAAT CCAGACGTCA CCCGTCCCGG TCAGAAAGAA TGGGCGCGAC 
GAGGAGAAGA GGGAGTCGCG CATCAAGACT TACTCGCCTT TTGCCTTCAA GTTCTACATG 
GAGCAGCACA TCGAGAACGT GATGAAGACC TACAACCAGA AACTGAACCG CAGGCTTCAG 
CTGGAGCAAG AGATGTCCAA GGCCGGCCTT TCGGAAGCGG AACAAGAACA GATGAGGAAG 
ATGCTGAACC AGAAAGAGTC CAATTATAAC AGGCTGCGCC GTGCCAAAAT GGACAAGTCT 
ATGTTTGTGA AAATCAAGAC GTTGGGCATA GGAGCCTTTG GGGAAGTGTG CCTCACACGT 
AAGGTGGACA CCTGTGCGCT TTATGCCATG AAAACACTGC GGAAGAAAGA CGTCCTCAAC 
CGCAATCAGG TGGCCCACGT GAAAGCAGAA CGCGACATCC TGGCTGAGGC AGACAACGAG 
TGGGTGGTGC GTCTCTACTA CTCCTTCCAG GATCGAGAGA GCCTCTACTT TGTCATGGAC 
TACATACCCG GGGGAGATAT GATGAGCCTG CTCATCCGAA TGGGGGTCTT CCCTGAGATC 
CTGGCGTGTT TCTACGTGGC GGAGCTGACG CTGGCCATCG AGAGCGTCCA CAAGATGGGC 
TTTATCCACC GCGACATCAA GCCCGACAAC ATCCTCATTG ATCTGGACGG ACACATAAAG 
CTGACGGACT TTGGCCTATG TACGGGATTC CGCTGGACGC ACAACTCCAA GTATTACCAG 
AAAGGGAGCC ACGTGAGGCA GGACAGCATG GAGCCCAGCG ACTTTTGGGA CGACGTTTCC 
AACTGTCGCT GTGGCGACCG GCTGATGACG CTAGAGCAGC GCGCCAATCG TCAGCATCAG 
CGCTGCCTGG CTCATTCGCT GGTCGGAACG CCAAACTACA TCGCACCAGA GGTGCTGTTG 
CGTAAAGGTT ACACCCAGCT GTGTGACTGG TGGAGTGTGG GAGTGATCCT CTTCGAGATG 
CTGGTGGGAC AGCCGCCCTT CCTGGCTCCC ACACCCACTG AGACTCAGAT TAAGGTCATT 
AATTGGGAGA GTACCCTGCA GGTGCCCGCC CAAGTCAAAC TCAGTCCAGA GGCCATCGAC 
ATCATCGGCC GCCTCTGCTG CTCTGCAGAA GATCGTCTGG GTGCCAACGG GGCTGGCGAG 
ATAAAAGCCC ACCCCTTTTT CTCCCAGACT GACTTCTCCA GCAACCTGCG GCAGCAGCCG 
GCCCCCTACC GCCCCAAGAT CGCCCATCCG ATGGACACGT CCAACTTCGA CCCCGTGGAG 
GAGGAAGGTG GCCCCGGAGC GTGGAGCGAC AGTGGGGACA GCACCCGGAC TTTGGACATG 
CTTTGCTCCC CTCAGGGGAA GCACCCAGAG CACGCCTTCT ACGAGTTCAC CTTCCGCAGA 
TTTTTTGACG ACAACGGCTG CCCCTTCCGC TACCCGAAGC CCCCGGAAGC CTGTGAGGCG 
TCGCCGCATA TGACAGGAGC CGGCTGCTTG GGCCCAGAGG AGGAGGACGA GCAAGAAGAA 
CAGGACACGG AAGATGAAGA GGAGGATGGA GAGCAGGGGG AGGGATGCGA GCCAGTCTAT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats2 ( XM_003962165.3 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003962214.1 Takifugu rubripes large tumor suppressor kinase 2 (lats2), mRNA.

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CloneID	OTo42617
Clone ID Related Accession (Same CDS sequence)	XM_003962165.3
Accession Version	XM_003962165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	serine/threonine-protein kinase LATS2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003962165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCCA AGACTTTCCC TGCTGCCCCG TATGTGGGCA ACACGCGCCA GAGGCTCCAG 
GAGATCAAGG AGGGTCTGAA GCAGCCAGCC AAGCTAGTGA GCCAGGCCCT GCATGGGGGC 
AGCTCTCGGG CGGAGGGCAG CAGAGGGGCT GACTGCAAAA GCGGGAAAGA CGCAGCCAGC 
CGACAGCAGC AGCTCCGTCC ACCGCAGAAG TTCAACAACT ACCAGAATGC CCTGAGAGAA 
ATTCGGAGGT CCCTCATGCC CTTTGCCAAC GAGTCCGGCC CGTCCTCTGG ATCGGGACAT 
CCTGCGGGGG CCGGGGAGGT GAACCGGCAG ATGCTCCAAG AGCTGGTCAA CGCTGGCTGC 
GACCAGGAGA TGGCAGTGCG GGCACTGAAG CAGACGGGCA GCCGGAACAT TGAAGCAGCC 
CTGGAGTACA TCAGTAAAAT GGGCTACCTT GATCCTCGCA ACGAGCTCAT CGTCCGCGTC 
ATCAAGCAGA CGTCACCAGG AAAAGCTGGC ATTCCAAACT CGATGGACCA CCGACCTTCC 
CTAGAAGCCT CAGGTGAAGC AGGGGCCATG CCTCCATACC ACCAGATGGG GGCTCCTATG 
TATGAGGGGC CAGCGGGATA CACCCCCGAT GGTGAGATCT CCCGACCTTA CATGAGCTCT 
GCCCCTGTGA TGAACTACAT GATGCCACCG ACAAGTTCAG CACAAGGACC GGCCATGGGA 
AACCCCATGG GTCGACCCCC GACTCTAGGC ACCTATCCAG CGGTGATGGG CAGCCAGGGC 
AACCCGGCCA ATACCATGTA CCCTCCAGGA GCCCAGCAGA AGAATTATCC AGGTAGCATG 
GAGCAGCATG GGCCCATGAT GAGCTACAAC GTTCCTGGGC AGCCTCTGCA GCTCCAACCT 
CAGCCTCCTG GGGGCCCTGT CCCAGGCCCA CATTATGACT ACAGCCATGC CAGGCCACAC 
ATGATGGATC CTGCAGGCTA TGGAGTGAAG CGGACCGCCT CCTTTCAGAA CAAGATGGCA 
CCGCCTCCCA TGGCACCCCC AGACAACTAC GTTAACATGC AAGGGAAAGG TGCCATGGGT 
CAGAATGTTC CAGGGGGAGG AGGAGCTGGA GGGGGTGGAG GGTATCCTGC CAACCGGTAC 
CTGCCCCCAC ATTCCCACCC ACGCCAGGCC AGCCCCACCT CCCACCAGGT TCACCTGATG 
TCCCGATCCC CGGGAGGGGT AGCAGCCATG GGCCCTGACT TCTCTGACAT ACCCCAAGGC 
TTGATGACGC CGTCCAGGGC CAGTCTTAAC CTGGACATTT ATGAACACCA CTGGGCTGGA 
CCTCCGGGCC CTGAAGGGGC CCCGCCAGGT AGGCAACCCC AACCACAAGG CCCATTCAGG 
GGTGAAGTTC GTGTCCCGAG CCGAACCAAC TCCTTCAACA GCCGTTCTGC TGGGCCCAAT 
GGCGTCCGAC CAACTCTGAC TGCACCCCCG ACAGCTGGGA AACAAGATCC TTCTCTGGGC 
CCACCTAACA CGATCACCGC TGTGACGTCC CCCCCAATCC AGCAGCCAGT CAAGAGCATC 
CGTGTAATGA GGCCAGAGCC CAAGACGGCT GTGGGACCAT GTCACCCTGG CTGGATGGCC 
GCTCAGACCC AGGATGCCGG CGAGCCTCTG GCGTATATGG CAGAGGAACC GTACCCCATT 
GAGCCGGCTC AGGAGCCACG CTGCCCACCA CCCCCTTATC CCAAAAACCT GCTCATGCCC 
GGCCCAACTG CAGAACCGGG GGGCTTGGAA GCAGCCGGAG CCATGTGTGG AGCTCAGGAC 
ATCGGCAACG CCGCCGTCAG AAGTACACAC GGCAGCGGCA GTGGAGGGAG CGGGAAGGTG 
GAGGAGAGCC ATCAGGTGAA AGAGAAGACG AAGATGTGCA AGACGGACAA AACCGATAAA 
ACTGTAAAAG ACAAGAAGCA AATCCAGACG TCACCCGTCC CGGTCAGAAA GAATGGGCGC 
GACGAGGAGA AGAGGGAGTC GCGCATCAAG ACTTACTCGC CTTTTGCCTT CAAGTTCTAC 
ATGGAGCAGC ACATCGAGAA CGTGATGAAG ACCTACAACC AGAAACTGAA CCGCAGGCTT 
CAGCTGGAGC AAGAGATGTC CAAGGCCGGC CTTTCGGAAG CGGAACAAGA ACAGATGAGG 
AAGATGCTGA ACCAGAAAGA GTCCAATTAT AACAGGCTGC GCCGTGCCAA AATGGACAAG 
TCTATGTTTG TGAAAATCAA GACGTTGGGC ATAGGAGCCT TTGGGGAAGT GTGCCTCACA 
CGTAAGGTGG ACACCTGTGC GCTTTATGCC ATGAAAACAC TGCGGAAGAA AGACGTCCTC 
AACCGCAATC AGGTGGCCCA CGTGAAAGCA GAACGCGACA TCCTGGCTGA GGCAGACAAC 
GAGTGGGTGG TGCGTCTCTA CTACTCTTTC CAGGATCGAG AGAGCCTCTA CTTTGTCATG 
GACTACATAC CCGGGGGAGA TATGATGAGC CTGCTCATCC GAATGGGGGT CTTCCCTGAG 
ATCCTGGCGT GTTTCTACGT GGCGGAGCTG ACGCTGGCCA TCGAGAGCGT CCACAAGATG 
GGCTTTATCC ACCGCGACAT CAAGCCCGAC AACATCCTCA TTGATCTGGA CGGACACATA 
AAGCTGACGG ACTTTGGCCT ATGTACGGGA TTCCGCTGGA CGCACAACTC CAAGTATTAC 
CAGAAAGGGA GCCACGTGAG GCAGGACAGC ATGGAGCCCA GCGACTTTTG GGACGACGTT 
TCCAACTGTC GCTGTGGCGA CCGGCTGATG ACGCTAGAGC AGCGCGCCAA TCGTCAGCAT 
CAGCGCTGCC TGGCTCATTC GCTGGTCGGA ACGCCAAACT ACATCGCACC AGAGGTGCTG 
TTGCGTAAAG GTTACACCCA GCTGTGTGAC TGGTGGAGTG TGGGAGTGAT CCTCTTCGAG 
ATGCTGGTGG GACAGCCGCC CTTCCTGGCT CCCACACCCA CTGAGACTCA GATTAAGGTC 
ATTAATTGGG AGAGTACCCT GCAGGTGCCC GCCCAAGTCA AACTCAGTCC AGAGGCCATC 
GACATCATCG GCCGCCTCTG CTGCTCTGCA GAAGATCGTC TGGGTGCCAA CGGGGCTGGC 
GAGATAAAAG CCCACCCCTT TTTCTCCCAG ACTGACTTCT CCAGCAACCT GCGGCAGCAG 
CCGGCCCCCT ACCGCCCCAA GATCGCCCAT CCGATGGACA CGTCCAACTT CGACCCCGTG 
GAGGAGGAAG GTGGCCCCGG AGCGTGGAGC GACAGTGGGG ACAGCACCCG GACTTTGGAC 
ATGCTTTGCT CCCCTCAGGG GAAGCACCCA GAGCACGCCT TCTACGAGTT CACCTTCCGC 
AGATTTTTTG ACGACAACGG CTGCCCCTTC CGCTACCCGA AGCCCCCGGA AGCCTGTGAG 
GCGTCGCCGC ATATGACAGG AGCCGGCTGC TTGGGCCCAG AGGAGGAGGA CGAGCAAGAA 
GAACAGGACA CGGAAGATGA AGAGGAGGAT GGAGAGCAGG GGGAGGGATG CGAGCCAGTC 
TATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003962214.1
CDS	596..4144
Translation	MRPKTFPAAPYVGNTRQRLQEIKEGLKQPAKLVSQALHGGSSRAEGSRGADCKSGKDAASRQQQLRPPQKFNNYQNALREIRRSLMPFANESGPSSGSGHPAGAGEVNRQMLQELVNAGCDQEMAVRALKQTGSRNIEAALEYISKMGYLDPRNELIVRVIKQTSPGKAGIPNSMDHRPSLEASGEAGAMPPYHQMGAPMYEGPAGYTPDGEISRPYMSSAPVMNYMMPPTSSAQGPAMGNPMGRPPTLGTYPAVMGSQGNPANTMYPPGAQQKNYPGSMEQHGPMMSYNVPGQPLQLQPQPPGGPVPGPHYDYSHARPHMMDPAGYGVKRTASFQNKMAPPPMAPPDNYVNMQGKGAMGQNVPGGGGAGGGGGYPANRYLPPHSHPRQASPTSHQVHLMSRSPGGVAAMGPDFSDIPQGLMTPSRASLNLDIYEHHWAGPPGPEGAPPGRQPQPQGPFRGEVRVPSRTNSFNSRSAGPNGVRPTLTAPPTAGKQDPSLGPPNTITAVTSPPIQQPVKSIRVMRPEPKTAVGPCHPGWMAAQTQDAGEPLAYMAEEPYPIEPAQEPRCPPPPYPKNLLMPGPTAEPGGLEAAGAMCGAQDIGNAAVRSTHGSGSGGSGKVEESHQVKEKTKMCKTDKTDKTVKDKKQIQTSPVPVRKNGRDEEKRESRIKTYSPFAFKFYMEQHIENVMKTYNQKLNRRLQLEQEMSKAGLSEAEQEQMRKMLNQKESNYNRLRRAKMDKSMFVKIKTLGIGAFGEVCLTRKVDTCALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRESLYFVMDYIPGGDMMSLLIRMGVFPEILACFYVAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSDFWDDVSNCRCGDRLMTLEQRANRQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQIKVINWESTLQVPAQVKLSPEAIDIIGRLCCSAEDRLGANGAGEIKAHPFFSQTDFSSNLRQQPAPYRPKIAHPMDTSNFDPVEEEGGPGAWSDSGDSTRTLDMLCSPQGKHPEHAFYEFTFRRFFDDNGCPFRYPKPPEACEASPHMTGAGCLGPEEEDEQEEQDTEDEEEDGEQGEGCEPVYV

Target ORF information:

RefSeq Version	XM_003962165.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes large tumor suppressor kinase 2 (lats2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003962165.3

ORF Insert Sequence:

ATGAGGCCCA AGACTTTCCC TGCTGCCCCG TATGTGGGCA ACACGCGCCA GAGGCTCCAG 
GAGATCAAGG AGGGTCTGAA GCAGCCAGCC AAGCTAGTGA GCCAGGCCCT GCATGGGGGC 
AGCTCTCGGG CGGAGGGCAG CAGAGGGGCT GACTGCAAAA GCGGGAAAGA CGCAGCCAGC 
CGACAGCAGC AGCTCCGTCC ACCGCAGAAG TTCAACAACT ACCAGAATGC CCTGAGAGAA 
ATTCGGAGGT CCCTCATGCC CTTTGCCAAC GAGTCCGGCC CGTCCTCTGG ATCGGGACAT 
CCTGCGGGGG CCGGGGAGGT GAACCGGCAG ATGCTCCAAG AGCTGGTCAA CGCTGGCTGC 
GACCAGGAGA TGGCAGTGCG GGCACTGAAG CAGACGGGCA GCCGGAACAT TGAAGCAGCC 
CTGGAGTACA TCAGTAAAAT GGGCTACCTT GATCCTCGCA ACGAGCTCAT CGTCCGCGTC 
ATCAAGCAGA CGTCACCAGG AAAAGCTGGC ATTCCAAACT CGATGGACCA CCGACCTTCC 
CTAGAAGCCT CAGGTGAAGC AGGGGCCATG CCTCCATACC ACCAGATGGG GGCTCCTATG 
TATGAGGGGC CAGCGGGATA CACCCCCGAT GGTGAGATCT CCCGACCTTA CATGAGCTCT 
GCCCCTGTGA TGAACTACAT GATGCCACCG ACAAGTTCAG CACAAGGACC GGCCATGGGA 
AACCCCATGG GTCGACCCCC GACTCTAGGC ACCTATCCAG CGGTGATGGG CAGCCAGGGC 
AACCCGGCCA ATACCATGTA CCCTCCAGGA GCCCAGCAGA AGAATTATCC AGGTAGCATG 
GAGCAGCATG GGCCCATGAT GAGCTACAAC GTTCCTGGGC AGCCTCTGCA GCTCCAACCT 
CAGCCTCCTG GGGGCCCTGT CCCAGGCCCA CATTATGACT ACAGCCATGC CAGGCCACAC 
ATGATGGATC CTGCAGGCTA TGGAGTGAAG CGGACCGCCT CCTTTCAGAA CAAGATGGCA 
CCGCCTCCCA TGGCACCCCC AGACAACTAC GTTAACATGC AAGGGAAAGG TGCCATGGGT 
CAGAATGTTC CAGGGGGAGG AGGAGCTGGA GGGGGTGGAG GGTATCCTGC CAACCGGTAC 
CTGCCCCCAC ATTCCCACCC ACGCCAGGCC AGCCCCACCT CCCACCAGGT TCACCTGATG 
TCCCGATCCC CGGGAGGGGT AGCAGCCATG GGCCCTGACT TCTCTGACAT ACCCCAAGGC 
TTGATGACGC CGTCCAGGGC CAGTCTTAAC CTGGACATTT ATGAACACCA CTGGGCTGGA 
CCTCCGGGCC CTGAAGGGGC CCCGCCAGGT AGGCAACCCC AACCACAAGG CCCATTCAGG 
GGTGAAGTTC GTGTCCCGAG CCGAACCAAC TCCTTCAACA GCCGTTCTGC TGGGCCCAAT 
GGCGTCCGAC CAACTCTGAC TGCACCCCCG ACAGCTGGGA AACAAGATCC TTCTCTGGGC 
CCACCTAACA CGATCACCGC TGTGACGTCC CCCCCAATCC AGCAGCCAGT CAAGAGCATC 
CGTGTAATGA GGCCAGAGCC CAAGACGGCT GTGGGACCAT GTCACCCTGG CTGGATGGCC 
GCTCAGACCC AGGATGCCGG CGAGCCTCTG GCGTATATGG CAGAGGAACC GTACCCCATT 
GAGCCGGCTC AGGAGCCACG CTGCCCACCA CCCCCTTATC CCAAAAACCT GCTCATGCCC 
GGCCCAACTG CAGAACCGGG GGGCTTGGAA GCAGCCGGAG CCATGTGTGG AGCTCAGGAC 
ATCGGCAACG CCGCCGTCAG AAGTACACAC GGCAGCGGCA GTGGAGGGAG CGGGAAGGTG 
GAGGAGAGCC ATCAGGTGAA AGAGAAGACG AAGATGTGCA AGACGGACAA AACCGATAAA 
ACTGTAAAAG ACAAGAAGCA AATCCAGACG TCACCCGTCC CGGTCAGAAA GAATGGGCGC 
GACGAGGAGA AGAGGGAGTC GCGCATCAAG ACTTACTCGC CTTTTGCCTT CAAGTTCTAC 
ATGGAGCAGC ACATCGAGAA CGTGATGAAG ACCTACAACC AGAAACTGAA CCGCAGGCTT 
CAGCTGGAGC AAGAGATGTC CAAGGCCGGC CTTTCGGAAG CGGAACAAGA ACAGATGAGG 
AAGATGCTGA ACCAGAAAGA GTCCAATTAT AACAGGCTGC GCCGTGCCAA AATGGACAAG 
TCTATGTTTG TGAAAATCAA GACGTTGGGC ATAGGAGCCT TTGGGGAAGT GTGCCTCACA 
CGTAAGGTGG ACACCTGTGC GCTTTATGCC ATGAAAACAC TGCGGAAGAA AGACGTCCTC 
AACCGCAATC AGGTGGCCCA CGTGAAAGCA GAACGCGACA TCCTGGCTGA GGCAGACAAC 
GAGTGGGTGG TGCGTCTCTA CTACTCTTTC CAGGATCGAG AGAGCCTCTA CTTTGTCATG 
GACTACATAC CCGGGGGAGA TATGATGAGC CTGCTCATCC GAATGGGGGT CTTCCCTGAG 
ATCCTGGCGT GTTTCTACGT GGCGGAGCTG ACGCTGGCCA TCGAGAGCGT CCACAAGATG 
GGCTTTATCC ACCGCGACAT CAAGCCCGAC AACATCCTCA TTGATCTGGA CGGACACATA 
AAGCTGACGG ACTTTGGCCT ATGTACGGGA TTCCGCTGGA CGCACAACTC CAAGTATTAC 
CAGAAAGGGA GCCACGTGAG GCAGGACAGC ATGGAGCCCA GCGACTTTTG GGACGACGTT 
TCCAACTGTC GCTGTGGCGA CCGGCTGATG ACGCTAGAGC AGCGCGCCAA TCGTCAGCAT 
CAGCGCTGCC TGGCTCATTC GCTGGTCGGA ACGCCAAACT ACATCGCACC AGAGGTGCTG 
TTGCGTAAAG GTTACACCCA GCTGTGTGAC TGGTGGAGTG TGGGAGTGAT CCTCTTCGAG 
ATGCTGGTGG GACAGCCGCC CTTCCTGGCT CCCACACCCA CTGAGACTCA GATTAAGGTC 
ATTAATTGGG AGAGTACCCT GCAGGTGCCC GCCCAAGTCA AACTCAGTCC AGAGGCCATC 
GACATCATCG GCCGCCTCTG CTGCTCTGCA GAAGATCGTC TGGGTGCCAA CGGGGCTGGC 
GAGATAAAAG CCCACCCCTT TTTCTCCCAG ACTGACTTCT CCAGCAACCT GCGGCAGCAG 
CCGGCCCCCT ACCGCCCCAA GATCGCCCAT CCGATGGACA CGTCCAACTT CGACCCCGTG 
GAGGAGGAAG GTGGCCCCGG AGCGTGGAGC GACAGTGGGG ACAGCACCCG GACTTTGGAC 
ATGCTTTGCT CCCCTCAGGG GAAGCACCCA GAGCACGCCT TCTACGAGTT CACCTTCCGC 
AGATTTTTTG ACGACAACGG CTGCCCCTTC CGCTACCCGA AGCCCCCGGA AGCCTGTGAG 
GCGTCGCCGC ATATGACAGG AGCCGGCTGC TTGGGCCCAG AGGAGGAGGA CGAGCAAGAA 
GAACAGGACA CGGAAGATGA AGAGGAGGAT GGAGAGCAGG GGGAGGGATG CGAGCCAGTC 
TATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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lats2 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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