LOC101071575 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101071575
Entrez Gene ID	101071575
Full Name	inactive phospholipase C-like protein 2
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003964879.2	XP_003964928.1	inactive phospholipase C-like protein 2
XM_003964879.3	XP_003964928.2	inactive phospholipase C-like protein 2

Pathways from BioSystems
Gene Ontology

The following LOC101071575 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101071575 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo03880	XM_003964879.2 Latest version!	Takifugu rubripes inactive phospholipase C-like protein 2 (LOC101071575), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo66496	XM_003964879.3 Latest version!	Takifugu rubripes inactive phospholipase C-like protein 2 (LOC101071575), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101071575 ( XM_003964879.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003964928.1 Takifugu rubripes inactive phospholipase C-like protein 2 (LOC101071575), mRNA.

Price & Availability↑

CloneID	OTo03880
Clone ID Related Accession (Same CDS sequence)	XM_003964879.2
Accession Version	XM_003964879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	inactive phospholipase C-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070961.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003964879.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG TACAAGCTTC AGAGGCTGCG TCGGGACATT ATTTGGATCC GGCCATCGGC 
TCCATGCTGA CCCAGGGCGC GACGAGCGCA CCGGGAGTTA CCGGCGGCTC GGCCGCGCTT 
CCTCCAGCCG GAGCACGGGC TTACTTGGAA GTGTCAGCCG CTGCTCAGGG CTACGGAGCC 
GAGGGAGTTT ACAGCCGGTC CAGGGAAGAC AGCAGCCCGA GGATCGGCAG GGGAAGCCGC 
GATAATTCGG CTGACAGGAG TCCGGCAGCT GGAAACGCAC CTTGCGGCAT CATGAAGGAT 
GGCTCCAAAC AGAGGCAGGC GAGAAAGAAA ACAGTGTCCT TCAGCACGAT GCCAAGCGAC 
CGCAAGATCA ACAGCACAGC TGCTTGCATG GCGTTCATGA TGGAGGGCTG TGAAATGAAA 
AAGGTCCGCT CCAACTCGCG CATGTACAAC CGATACTTCT TGCTGGATCC CGACATGCAC 
TGGCTCCGAT GGGAGCCGTC CAAGAAGGAT TCGGAGAAAG CCAAGCTGGA GATCAAGGCT 
ATTAAGGAGG TTCGTTTGGG GAAGAAAACT CCAGTTCTGC GCAGCAATGG ACTCTCAGAT 
CAGTTCCCAG ATGAATGCGC CTTTTCAATC ATATATGGGG ATAACTATGA GTCTTTAGAT 
TTAGTGGCCA GCACCGCAGA TGTTGTCAGT ACCTGGGTGA TGGGTCTGCG TTATCTGGTA 
TCTTATGGCC GGCATACAGT GAACATGGTA GAGCCCAACC AACCGAGTCT GAGGACTTCC 
TGGATTAGCT CAGTCTTTGA GCTGGCAGAT ACAGAAAAAG CAGGACACAT AGACCTCTTC 
AGGGCCACCC AGATAATCAA GGGGCTCAAC GCTGGAATGA AAGAGTCCAG AATAGAGCTG 
AAGTTTAAAG AACTTCAGAA AGCTAAAGAC CAATATGGGG AGGCAATTAA CATGGACATT 
TTCGTGGAGG CCTATTGTGA GCTCTGCACT CGACCGGAGA TTTTCTTTCT GCTAATGCAG 
TTTTCCAGTA ACAAGGAGTA TCTGGACAGT AAAGATCTAA TGCTATTTGT GGAAGTGGAG 
CAAGGTGTGG AAGGTGTGAC AGAGGACATG TGCAGGGATA TTGTTCAGAA ATTTGAGCCA 
TCAGCCGAAG GCAGGGAGCG GTCGTATCTC TCCATCGATG GCTTCACACA CTACCTCCTC 
TCCTCTGAAT GCCACATCTT TGACCCCCAG CACAAACGCG TGTGCCAGGA CATGCACCAG 
CCACTGTCCC ACTACTACAT CAACTCCTCC CATAATGCCT CCCTTTTGGA AGATCATTTC 
TGGGGCTCGT CAGACATCAG CAGCTACATC AGAGCTCTGA GGATGGGATG TCGCAGCATT 
GAAGTTATTG TATGGGATGG CCCTGATAAT GAGCCTGTGG TGTATGTTGG CAGTTCTGTC 
GCCTCACATC TGGTTTTCTC CAAAATTCTC GACATTATAA ACCAGTATGC TTTCGAGAGC 
TCCGAGTACC CCCTGATTCT CTGCTTGGTG ACCCACTGCT GCATCCTCCA GCAGAGGGTC 
ATGGCACAAC ACATGAAGAA GATTTTTGGG GACAAGCTGT ATGTTGATCC TCCAAACAAG 
GAGGAAAACT ATCTTCTCTC CCCAGAGAAA CTCAAAGGCA AAATCCTCCT CAAGGGTAAA 
AAACTACCAC CCAGCTGTAC CGATGCTGAG GGGGATGTAA CAGATGAGGA AGAAGGTCTG 
GAAATGTCTC GGAGATTAGG ATCTGATGAA AAGGACCAAC TAAATGGACT CGGCTACAAG 
AGACTTAGAT TGTGCCGGGA ACTGTCTGAG CTTGTGTCTC TCTGCAAGTC AGTCCAGTTC 
AGAGACTTTG AAATCACAAA GAGGGAGCAA AAATACTGGG AGATGTGCTC CTTCAATGAG 
GTGGATGCAA ACAGATATGC CAATGAGTTC CCAGAGGAAT TTGTTTGTTA TAACAAGCGC 
TTTCTCTCCA GAGTGTATCC CACACCTATG AGGATTGATG CCAGCAACAT GAACCCCCAG 
GATTTCTGGA AGTGTGGCTG CCAAATAGTG GCCATGAACT ACCAGACGCC AGGTCTGATG 
ATGGACCTCA ACCTAGGTTG GTTCAGACAG AATGGAAACT GTGGCTATGT TTTACGTCCT 
GCAATTATGA GAGAGGAGGT GTCCTACTTC AGTGCCAATG CTCGAGATTC CCTGCCAGGA 
GTGTCTGCCC AGCTTCTCCA CATAAAAGTC ATCAGCGGAC AGAACCTGCC CAAGCCTCGA 
GGATCTGCCG CCAAGGGTGA TGTGGTGGAG CCCTACATTT ATGTGGAGAT CCACGGTATC 
CCAGCTGACT GTGCTGAGCA GAGAACCAAA ACTGTCTCTC AGAATGGCGA CAACCCTATT 
TTCGACGAGA GTTTTGAATT TCAGATTAAT TTACCAGAAC TCGCCGTGCT GCGCTTTGTG 
GTGCTGGACG ACGATTACAT CGGAGATGAG TTCATCGGCC AGTACACCAT ACCGTTTGAG 
TGCCTACAGC CAGGCTATAG ACATGTCCCG CTGCAGTCTC TGACTGGAGA GTTCCTACCA 
AACACCACCT TGTTTGTTCA CGTGGCCATC ACCAACAGAC GTGGTGGAGG AAAGGCACAT 
AAGAGGGGTC TGTCTGTGAG GAAGGGGAGG AAGACCCGGG AATACACCAC CACCAAGTCC 
ACGGGGATCA AGATAGTGGA TGAGCTCTTC AGAGCTTCCA CTCAGCCCCT CAGGGAAGCC 
ACAGACCTCA GAGAGAATGT GCAGAATGCT GTGGTTTCCT TCAAGGAGCT GTGTGGCCTG 
ACTCCAGCGG CCAACATGAA GCAGTGTATC CTGACCGTGT CTACCTGGCT GATGAACAGT 
GAGCGGTCCC TGAGGGTAAC CGTGGACCTG AGCGAGACTT ACCCCACCAT GGAAGCTCAA 
GGTCCTGTCC CTGAACTGCT GCGGAAAGTG CTCAATGCTT ACGACATGAT GATCCAAACC 
AGCAGAACAC TGATCGAGTC AGCTGATGTC ATCTACTCCA AGCTCACACA AGCCCAGCGA 
GCAGGTTTGG ACTTCCACGA GGACCTGCAC CGGATTGGCG CCAAGGAGGG CCTGAAGGGG 
CGTAAACTTC AAAAGGCCAT GGAGAGCTAT GCTTGGAACA TCACTGTCCT CAAGGGCCAA 
GCGGACCTGC TGAAGCACGC TAAGAGCGAG GCACTGGACA CACTGCGTCA GATCCACTGT 
GCAGCTCAGT CCTGCGGTCT CAGTAAGAAT GAAGCGGCTT CCCCACAGGA GCAACACCAT 
CCTCAGCAGC AGCTCCAGCA GCTCCAGCAG CTCCCGCAGC TCCAGATTCA GCAACAGTCT 
ACGGCTAAGG CACTGTCACA GCCCACCTCG CTGTACCTGG CTCAACCACA GACTACACCT 
CAGGCTTATA CCCTTCCTCT TCCTCAGCAA CATCCTCTGT TACCAGCTCA CACTCTGGTT 
CAGACTGTTC TAAAACCACC AGCGTATTCT CACCCACCAC CCCTGCCCCA GGGTCCGTTA 
CCACCCCAAG GGCCACAGCA GAGGGCAGCG GGTCCTTTGG ACGTCATCCC CCAGAGAGAG 
GTGGCCAGTG ACAGTCCTCC AGGATCCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003964928.1
CDS	484..4116
Translation	MAEVQASEAASGHYLDPAIGSMLTQGATSAPGVTGGSAALPPAGARAYLEVSAAAQGYGAEGVYSRSREDSSPRIGRGSRDNSADRSPAAGNAPCGIMKDGSKQRQARKKTVSFSTMPSDRKINSTAACMAFMMEGCEMKKVRSNSRMYNRYFLLDPDMHWLRWEPSKKDSEKAKLEIKAIKEVRLGKKTPVLRSNGLSDQFPDECAFSIIYGDNYESLDLVASTADVVSTWVMGLRYLVSYGRHTVNMVEPNQPSLRTSWISSVFELADTEKAGHIDLFRATQIIKGLNAGMKESRIELKFKELQKAKDQYGEAINMDIFVEAYCELCTRPEIFFLLMQFSSNKEYLDSKDLMLFVEVEQGVEGVTEDMCRDIVQKFEPSAEGRERSYLSIDGFTHYLLSSECHIFDPQHKRVCQDMHQPLSHYYINSSHNASLLEDHFWGSSDISSYIRALRMGCRSIEVIVWDGPDNEPVVYVGSSVASHLVFSKILDIINQYAFESSEYPLILCLVTHCCILQQRVMAQHMKKIFGDKLYVDPPNKEENYLLSPEKLKGKILLKGKKLPPSCTDAEGDVTDEEEGLEMSRRLGSDEKDQLNGLGYKRLRLCRELSELVSLCKSVQFRDFEITKREQKYWEMCSFNEVDANRYANEFPEEFVCYNKRFLSRVYPTPMRIDASNMNPQDFWKCGCQIVAMNYQTPGLMMDLNLGWFRQNGNCGYVLRPAIMREEVSYFSANARDSLPGVSAQLLHIKVISGQNLPKPRGSAAKGDVVEPYIYVEIHGIPADCAEQRTKTVSQNGDNPIFDESFEFQINLPELAVLRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLQSLTGEFLPNTTLFVHVAITNRRGGGKAHKRGLSVRKGRKTREYTTTKSTGIKIVDELFRASTQPLREATDLRENVQNAVVSFKELCGLTPAANMKQCILTVSTWLMNSERSLRVTVDLSETYPTMEAQGPVPELLRKVLNAYDMMIQTSRTLIESADVIYSKLTQAQRAGLDFHEDLHRIGAKEGLKGRKLQKAMESYAWNITVLKGQADLLKHAKSEALDTLRQIHCAAQSCGLSKNEAASPQEQHHPQQQLQQLQQLPQLQIQQQSTAKALSQPTSLYLAQPQTTPQAYTLPLPQQHPLLPAHTLVQTVLKPPAYSHPPPLPQGPLPPQGPQQRAAGPLDVIPQREVASDSPPGSC

Target ORF information:

RefSeq Version	XM_003964879.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes inactive phospholipase C-like protein 2 (LOC101071575), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003964879.2

ORF Insert Sequence:

ATGGCAGAGG TACAAGCTTC AGAGGCTGCG TCGGGACATT ATTTGGATCC GGCCATCGGC 
TCCATGCTGA CCCAGGGCGC GACGAGCGCA CCGGGAGTTA CCGGCGGCTC GGCCGCGCTT 
CCTCCAGCCG GAGCACGGGC TTACTTGGAA GTGTCAGCCG CTGCTCAGGG CTACGGAGCC 
GAGGGAGTTT ACAGCCGGTC CAGGGAAGAC AGCAGCCCGA GGATCGGCAG GGGAAGCCGC 
GATAATTCGG CTGACAGGAG TCCGGCAGCT GGAAACGCAC CTTGCGGCAT CATGAAGGAT 
GGCTCCAAAC AGAGGCAGGC GAGAAAGAAA ACAGTGTCCT TCAGCACGAT GCCAAGCGAC 
CGCAAGATCA ACAGCACAGC TGCTTGCATG GCGTTCATGA TGGAGGGCTG TGAAATGAAA 
AAGGTCCGCT CCAACTCGCG CATGTACAAC CGATACTTCT TGCTGGATCC CGACATGCAC 
TGGCTCCGAT GGGAGCCGTC CAAGAAGGAT TCGGAGAAAG CCAAGCTGGA GATCAAGGCT 
ATTAAGGAGG TTCGTTTGGG GAAGAAAACT CCAGTTCTGC GCAGCAATGG ACTCTCAGAT 
CAGTTCCCAG ATGAATGCGC CTTTTCAATC ATATATGGGG ATAACTATGA GTCTTTAGAT 
TTAGTGGCCA GCACCGCAGA TGTTGTCAGT ACCTGGGTGA TGGGTCTGCG TTATCTGGTA 
TCTTATGGCC GGCATACAGT GAACATGGTA GAGCCCAACC AACCGAGTCT GAGGACTTCC 
TGGATTAGCT CAGTCTTTGA GCTGGCAGAT ACAGAAAAAG CAGGACACAT AGACCTCTTC 
AGGGCCACCC AGATAATCAA GGGGCTCAAC GCTGGAATGA AAGAGTCCAG AATAGAGCTG 
AAGTTTAAAG AACTTCAGAA AGCTAAAGAC CAATATGGGG AGGCAATTAA CATGGACATT 
TTCGTGGAGG CCTATTGTGA GCTCTGCACT CGACCGGAGA TTTTCTTTCT GCTAATGCAG 
TTTTCCAGTA ACAAGGAGTA TCTGGACAGT AAAGATCTAA TGCTATTTGT GGAAGTGGAG 
CAAGGTGTGG AAGGTGTGAC AGAGGACATG TGCAGGGATA TTGTTCAGAA ATTTGAGCCA 
TCAGCCGAAG GCAGGGAGCG GTCGTATCTC TCCATCGATG GCTTCACACA CTACCTCCTC 
TCCTCTGAAT GCCACATCTT TGACCCCCAG CACAAACGCG TGTGCCAGGA CATGCACCAG 
CCACTGTCCC ACTACTACAT CAACTCCTCC CATAATGCCT CCCTTTTGGA AGATCATTTC 
TGGGGCTCGT CAGACATCAG CAGCTACATC AGAGCTCTGA GGATGGGATG TCGCAGCATT 
GAAGTTATTG TATGGGATGG CCCTGATAAT GAGCCTGTGG TGTATGTTGG CAGTTCTGTC 
GCCTCACATC TGGTTTTCTC CAAAATTCTC GACATTATAA ACCAGTATGC TTTCGAGAGC 
TCCGAGTACC CCCTGATTCT CTGCTTGGTG ACCCACTGCT GCATCCTCCA GCAGAGGGTC 
ATGGCACAAC ACATGAAGAA GATTTTTGGG GACAAGCTGT ATGTTGATCC TCCAAACAAG 
GAGGAAAACT ATCTTCTCTC CCCAGAGAAA CTCAAAGGCA AAATCCTCCT CAAGGGTAAA 
AAACTACCAC CCAGCTGTAC CGATGCTGAG GGGGATGTAA CAGATGAGGA AGAAGGTCTG 
GAAATGTCTC GGAGATTAGG ATCTGATGAA AAGGACCAAC TAAATGGACT CGGCTACAAG 
AGACTTAGAT TGTGCCGGGA ACTGTCTGAG CTTGTGTCTC TCTGCAAGTC AGTCCAGTTC 
AGAGACTTTG AAATCACAAA GAGGGAGCAA AAATACTGGG AGATGTGCTC CTTCAATGAG 
GTGGATGCAA ACAGATATGC CAATGAGTTC CCAGAGGAAT TTGTTTGTTA TAACAAGCGC 
TTTCTCTCCA GAGTGTATCC CACACCTATG AGGATTGATG CCAGCAACAT GAACCCCCAG 
GATTTCTGGA AGTGTGGCTG CCAAATAGTG GCCATGAACT ACCAGACGCC AGGTCTGATG 
ATGGACCTCA ACCTAGGTTG GTTCAGACAG AATGGAAACT GTGGCTATGT TTTACGTCCT 
GCAATTATGA GAGAGGAGGT GTCCTACTTC AGTGCCAATG CTCGAGATTC CCTGCCAGGA 
GTGTCTGCCC AGCTTCTCCA CATAAAAGTC ATCAGCGGAC AGAACCTGCC CAAGCCTCGA 
GGATCTGCCG CCAAGGGTGA TGTGGTGGAG CCCTACATTT ATGTGGAGAT CCACGGTATC 
CCAGCTGACT GTGCTGAGCA GAGAACCAAA ACTGTCTCTC AGAATGGCGA CAACCCTATT 
TTCGACGAGA GTTTTGAATT TCAGATTAAT TTACCAGAAC TCGCCGTGCT GCGCTTTGTG 
GTGCTGGACG ACGATTACAT CGGAGATGAG TTCATCGGCC AGTACACCAT ACCGTTTGAG 
TGCCTACAGC CAGGCTATAG ACATGTCCCG CTGCAGTCTC TGACTGGAGA GTTCCTACCA 
AACACCACCT TGTTTGTTCA CGTGGCCATC ACCAACAGAC GTGGTGGAGG AAAGGCACAT 
AAGAGGGGTC TGTCTGTGAG GAAGGGGAGG AAGACCCGGG AATACACCAC CACCAAGTCC 
ACGGGGATCA AGATAGTGGA TGAGCTCTTC AGAGCTTCCA CTCAGCCCCT CAGGGAAGCC 
ACAGACCTCA GAGAGAATGT GCAGAATGCT GTGGTTTCCT TCAAGGAGCT GTGTGGCCTG 
ACTCCAGCGG CCAACATGAA GCAGTGTATC CTGACCGTGT CTACCTGGCT GATGAACAGT 
GAGCGGTCCC TGAGGGTAAC CGTGGACCTG AGCGAGACTT ACCCCACCAT GGAAGCTCAA 
GGTCCTGTCC CTGAACTGCT GCGGAAAGTG CTCAATGCTT ACGACATGAT GATCCAAACC 
AGCAGAACAC TGATCGAGTC AGCTGATGTC ATCTACTCCA AGCTCACACA AGCCCAGCGA 
GCAGGTTTGG ACTTCCACGA GGACCTGCAC CGGATTGGCG CCAAGGAGGG CCTGAAGGGG 
CGTAAACTTC AAAAGGCCAT GGAGAGCTAT GCTTGGAACA TCACTGTCCT CAAGGGCCAA 
GCGGACCTGC TGAAGCACGC TAAGAGCGAG GCACTGGACA CACTGCGTCA GATCCACTGT 
GCAGCTCAGT CCTGCGGTCT CAGTAAGAAT GAAGCGGCTT CCCCACAGGA GCAACACCAT 
CCTCAGCAGC AGCTCCAGCA GCTCCAGCAG CTCCCGCAGC TCCAGATTCA GCAACAGTCT 
ACGGCTAAGG CACTGTCACA GCCCACCTCG CTGTACCTGG CTCAACCACA GACTACACCT 
CAGGCTTATA CCCTTCCTCT TCCTCAGCAA CATCCTCTGT TACCAGCTCA CACTCTGGTT 
CAGACTGTTC TAAAACCACC AGCGTATTCT CACCCACCAC CCCTGCCCCA GGGTCCGTTA 
CCACCCCAAG GGCCACAGCA GAGGGCAGCG GGTCCTTTGG ACGTCATCCC CCAGAGAGAG 
GTGGCCAGTG ACAGTCCTCC AGGATCCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071575 ( XM_003964879.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003964928.2 Takifugu rubripes inactive phospholipase C-like protein 2 (LOC101071575), mRNA.

Price & Availability↑

CloneID	OTo66496
Clone ID Related Accession (Same CDS sequence)	XM_003964879.3
Accession Version	XM_003964879.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	inactive phospholipase C-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042289.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003964879.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG TACAAGCTTC AGAGGCTGCG TCGGGACATT ATTTGGATCC GGCCATCGGC 
TCCATGCTGA CCCAGGGCGC GACGAGCGCA CCGGGAGTTA CCGGCGGCTC GGCCGCGCTT 
CCTCCAGCCG GAGCACGGGC TTACTTGGAA GTGTCAGCCG CTGCTCAGGG CTACGGAGCC 
GAGGGAGTTT ACAGCCGGTC CAGGGAAGAC AGCAGCCCGA GGATCGGCAG GGGAAGCCGC 
GATAATTCGG CTGACAGGAG TCCGGCAGCT GGAAACGCAC CTTGCGGCAT CATGAAGGAT 
GGCTCCAAAC AGAGGCAGGC GAGAAAGAAA ACAGTGTCCT TCAGCACGAT GCCAAGCGAC 
CGCAAGATCA ACAGCACAGC TGCTTGCATG GCGTTCATGA TGGAGGGCTG TGAAATGAAA 
AAGGTCCGCT CCAACTCGCG CATGTACAAC CGATACTTCT TGCTGGATCC CGACATGCAC 
TGGCTCCGAT GGGAGCCGTC CAAGAAGGAT TCGGAGAAAG CCAAGCTGGA GATCAAGGCT 
ATTAAGGAGG TTCGTTTGGG GAAGAAAACT CCAGTTCTGC GCAGCAATGG ACTCTCAGAT 
CAGTTCCCAG ATGAATGCGC CTTTTCAATC ATATATGGGG ATAACTATGA GTCTTTAGAT 
TTAGTGGCCA GCACCGCAGA TGTTGTCAGT ACCTGGGTGA TGGGTCTGCG TTATCTGGTA 
TCTTATGGCC GGCATACAGT GAACATGGTA GAGCCCAACC AACCGAGTCT GAGGACTTCC 
TGGATTAGCT CAGTCTTTGA GCTGGCAGAT ACAGAAAAAG CAGGACACAT AGACCTCTTC 
AGGGCCACCC AGATAATCAA GGGGCTCAAC GCTGGAATGA AAGAGTCCAG AATAGAGCTG 
AAGTTTAAAG AACTTCAGAA AGCTAAAGAC CAATATGGGG AGGCAATTAA CATGGACATT 
TTCGTGGAGG CCTATTGTGA GCTCTGCACT CGACCGGAGA TTTTCTTTCT GCTAATGCAG 
TTTTCCAGTA ACAAGGAGTA TCTGGACAGT AAAGATCTAA TGCTATTTGT GGAAGTGGAG 
CAAGGTGTGG AAGGTGTGAC AGAGGACATG TGCAGGGATA TTGTTCAGAA ATTTGAGCCA 
TCAGCCGAAG GCAGGGAGCG GTCGTATCTC TCCATCGATG GCTTCACACA CTACCTCCTC 
TCCTCTGAAT GCCACATCTT TGACCCCCAG CACAAACGCG TGTGCCAGGA CATGCACCAG 
CCACTGTCCC ACTACTACAT CAACTCCTCC CATAATGCCT CCCTTTTGGA AGATCATTTC 
TGGGGCTCGT CAGACATCAG CAGCTACATC AGAGCTCTGA GGATGGGATG TCGCAGCATT 
GAAGTTATTG TATGGGATGG CCCTGATAAT GAGCCTGTGG TGTATGTTGG CAGTTCTGTC 
GCCTCACATC TGGTTTTCTC CAAAATTCTC GACATTATAA ACCAGTATGC TTTCGAGAGC 
TCCGAGTACC CCCTGATTCT CTGCTTGGTG ACCCACTGCT GCATCCTCCA GCAGAGGGTC 
ATGGCACAAC ACATGAAGAA GATTTTTGGG GACAAGCTGT ATGTTGATCC TCCAAACAAG 
GAGGAAAACT ATCTTCTCTC CCCAGAGAAA CTCAAAGGCA AAATCCTCCT CAAGGGTAAA 
AAACTACCAC CCAGCTGTAC CGATGCTGAG GGGGATGTAA CAGATGAGGA AGAAGGTCTG 
GAAATGTCTC GGAGATTAGG ATCTGATGAA AAGGACCAAC TAAATGGACT CGGCTACAAG 
AGACTTAGAT TGTGCCGGGA ACTGTCTGAG CTTGTGTCTC TCTGCAAGTC AGTCCAGTTC 
AGAGACTTTG AAATCACAAA GAGGGAGCAA AAATACTGGG AGATGTGCTC CTTCAATGAG 
GTGGATGCAA ACAGATATGC CAATGAGTTC CCAGAGGAAT TTGTTTGTTA TAACAAGCGC 
TTTCTCTCCA GAGTGTATCC CACACCTATG AGGATTGATG CCAGCAACAT GAACCCCCAG 
GATTTCTGGA AGTGTGGCTG CCAAATAGTG GCCATGAACT ACCAGACGCC AGGTCTGATG 
ATGGACCTCA ACCTAGGTTG GTTCAGACAG AATGGAAACT GTGGCTATGT TTTACGTCCT 
GCAATTATGA GAGAGGAGGT GTCCTACTTC AGTGCCAATG CTCGAGATTC CCTGCCAGGA 
GTGTCTGCCC AGCTTCTCCA CATAAAAGTC ATCAGCGGAC AGAACCTGCC CAAGCCTCGA 
GGATCTGCCG CCAAGGGTGA TGTGGTGGAG CCCTACATTT ATGTGGAGAT CCACGGTATC 
CCAGCTGACT GTGCTGAGCA GAGAACCAAA ACTGTCTCTC AGAATGGCGA CAACCCTATT 
TTCGACGAGA GTTTTGAATT TCAGATTAAT TTACCAGAAC TCGCCGTGCT GCGCTTTGTG 
GTGCTGGACG ACGATTACAT CGGAGATGAG TTCATCGGCC AGTACACCAT ACCGTTTGAG 
TGCCTACAGC CCGGCTATAG ACATGTCCCG CTGCAGTCTC TGACTGGAGA GTTCCTACCA 
AACACCACCT TGTTTGTTCA CGTGGCCATC ACCAACAGAC GTGGTGGAGG AAAGGCTCAT 
AAGAGGGGTC TGTCTGTGAG GAAGGGGAGG AAGACCCGGG AATACACCAC CACCAAGTCC 
ACGGGGATCA AGATAGTGGA TGAGCTCTTC AGAGCTTCCA CTCAGCCCCT CAGGGAAGCC 
ACAGACCTCA GAGAGAATGT GCAGAATGCT GTGGTTTCCT TCAAGGAGCT GTGTGGCCTG 
ACTCCAGCGG CCAACATGAA GCAGTGTATC CTGACCGTGT CTACCTGGCT GATGAACAGT 
GAGCGGTCCC TGAGGGTAAC CGTGGACCTG AGCGAGACTT ACCCCACCAT GGAAGCTCAA 
GGTCCTGTCC CTGAACTGCT GCGGAAAGTG CTCAATGCTT ACGACATGAT GATCCAAACC 
AGCAGAACAC TGATCGAGTC AGCTGATGTC ATCTACTCCA AGCTCACACA AGCCCAGCGA 
GCAGGTTTGG ACTTCCACGA GGACCTGCAC CGGATTGGCG CCAAGGAGGG CCTGAAGGGG 
CGTAAACTTC AAAAGGCCAT GGAGAGCTAT GCTTGGAACA TCACTGTCCT CAAGGGCCAA 
GCGGACCTGC TGAAGCACGC TAAGAGCGAG GCGCTGGACA CACTGCGTCA GATCCACTGT 
GCAGCTCAGT CCTGCGGTCT CAGTAAGAAT GAAGCGGCTT CCCCACAGGA GCAACACCAT 
CCTCAGCAGC AGCCCCAGCA GCTCCAGCAG CTCCCGCAGC TCCAGATTCA GCAACAGTCT 
ACGGCTAAGG CACTGTCACA GCCCACCTCG CTGTACCTGG CTCAACCACA GACTACACCT 
CAGGCTTATA CCCTTCCTCT TCCTCAGCAA CATCCTCTGT TACCAGCTCA CACTCTGGTT 
CAGACTGTTC TAAAACCACC AGCGTATTCT CACCCACCAC CCCTGCCCCA GGGTCCGTTA 
CCACCCCAAG GGCCACAGCA GAGGGCAGCG GGTCCTTTGG ACGTCATCCC CCAGAGAGAG 
GTGGCCAGTG ACAGTCCTCC AGGATCCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003964928.2
CDS	679..4311
Translation	MAEVQASEAASGHYLDPAIGSMLTQGATSAPGVTGGSAALPPAGARAYLEVSAAAQGYGAEGVYSRSREDSSPRIGRGSRDNSADRSPAAGNAPCGIMKDGSKQRQARKKTVSFSTMPSDRKINSTAACMAFMMEGCEMKKVRSNSRMYNRYFLLDPDMHWLRWEPSKKDSEKAKLEIKAIKEVRLGKKTPVLRSNGLSDQFPDECAFSIIYGDNYESLDLVASTADVVSTWVMGLRYLVSYGRHTVNMVEPNQPSLRTSWISSVFELADTEKAGHIDLFRATQIIKGLNAGMKESRIELKFKELQKAKDQYGEAINMDIFVEAYCELCTRPEIFFLLMQFSSNKEYLDSKDLMLFVEVEQGVEGVTEDMCRDIVQKFEPSAEGRERSYLSIDGFTHYLLSSECHIFDPQHKRVCQDMHQPLSHYYINSSHNASLLEDHFWGSSDISSYIRALRMGCRSIEVIVWDGPDNEPVVYVGSSVASHLVFSKILDIINQYAFESSEYPLILCLVTHCCILQQRVMAQHMKKIFGDKLYVDPPNKEENYLLSPEKLKGKILLKGKKLPPSCTDAEGDVTDEEEGLEMSRRLGSDEKDQLNGLGYKRLRLCRELSELVSLCKSVQFRDFEITKREQKYWEMCSFNEVDANRYANEFPEEFVCYNKRFLSRVYPTPMRIDASNMNPQDFWKCGCQIVAMNYQTPGLMMDLNLGWFRQNGNCGYVLRPAIMREEVSYFSANARDSLPGVSAQLLHIKVISGQNLPKPRGSAAKGDVVEPYIYVEIHGIPADCAEQRTKTVSQNGDNPIFDESFEFQINLPELAVLRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLQSLTGEFLPNTTLFVHVAITNRRGGGKAHKRGLSVRKGRKTREYTTTKSTGIKIVDELFRASTQPLREATDLRENVQNAVVSFKELCGLTPAANMKQCILTVSTWLMNSERSLRVTVDLSETYPTMEAQGPVPELLRKVLNAYDMMIQTSRTLIESADVIYSKLTQAQRAGLDFHEDLHRIGAKEGLKGRKLQKAMESYAWNITVLKGQADLLKHAKSEALDTLRQIHCAAQSCGLSKNEAASPQEQHHPQQQPQQLQQLPQLQIQQQSTAKALSQPTSLYLAQPQTTPQAYTLPLPQQHPLLPAHTLVQTVLKPPAYSHPPPLPQGPLPPQGPQQRAAGPLDVIPQREVASDSPPGSC

Target ORF information:

RefSeq Version	XM_003964879.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes inactive phospholipase C-like protein 2 (LOC101071575), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003964879.3

ORF Insert Sequence:

ATGGCAGAGG TACAAGCTTC AGAGGCTGCG TCGGGACATT ATTTGGATCC GGCCATCGGC 
TCCATGCTGA CCCAGGGCGC GACGAGCGCA CCGGGAGTTA CCGGCGGCTC GGCCGCGCTT 
CCTCCAGCCG GAGCACGGGC TTACTTGGAA GTGTCAGCCG CTGCTCAGGG CTACGGAGCC 
GAGGGAGTTT ACAGCCGGTC CAGGGAAGAC AGCAGCCCGA GGATCGGCAG GGGAAGCCGC 
GATAATTCGG CTGACAGGAG TCCGGCAGCT GGAAACGCAC CTTGCGGCAT CATGAAGGAT 
GGCTCCAAAC AGAGGCAGGC GAGAAAGAAA ACAGTGTCCT TCAGCACGAT GCCAAGCGAC 
CGCAAGATCA ACAGCACAGC TGCTTGCATG GCGTTCATGA TGGAGGGCTG TGAAATGAAA 
AAGGTCCGCT CCAACTCGCG CATGTACAAC CGATACTTCT TGCTGGATCC CGACATGCAC 
TGGCTCCGAT GGGAGCCGTC CAAGAAGGAT TCGGAGAAAG CCAAGCTGGA GATCAAGGCT 
ATTAAGGAGG TTCGTTTGGG GAAGAAAACT CCAGTTCTGC GCAGCAATGG ACTCTCAGAT 
CAGTTCCCAG ATGAATGCGC CTTTTCAATC ATATATGGGG ATAACTATGA GTCTTTAGAT 
TTAGTGGCCA GCACCGCAGA TGTTGTCAGT ACCTGGGTGA TGGGTCTGCG TTATCTGGTA 
TCTTATGGCC GGCATACAGT GAACATGGTA GAGCCCAACC AACCGAGTCT GAGGACTTCC 
TGGATTAGCT CAGTCTTTGA GCTGGCAGAT ACAGAAAAAG CAGGACACAT AGACCTCTTC 
AGGGCCACCC AGATAATCAA GGGGCTCAAC GCTGGAATGA AAGAGTCCAG AATAGAGCTG 
AAGTTTAAAG AACTTCAGAA AGCTAAAGAC CAATATGGGG AGGCAATTAA CATGGACATT 
TTCGTGGAGG CCTATTGTGA GCTCTGCACT CGACCGGAGA TTTTCTTTCT GCTAATGCAG 
TTTTCCAGTA ACAAGGAGTA TCTGGACAGT AAAGATCTAA TGCTATTTGT GGAAGTGGAG 
CAAGGTGTGG AAGGTGTGAC AGAGGACATG TGCAGGGATA TTGTTCAGAA ATTTGAGCCA 
TCAGCCGAAG GCAGGGAGCG GTCGTATCTC TCCATCGATG GCTTCACACA CTACCTCCTC 
TCCTCTGAAT GCCACATCTT TGACCCCCAG CACAAACGCG TGTGCCAGGA CATGCACCAG 
CCACTGTCCC ACTACTACAT CAACTCCTCC CATAATGCCT CCCTTTTGGA AGATCATTTC 
TGGGGCTCGT CAGACATCAG CAGCTACATC AGAGCTCTGA GGATGGGATG TCGCAGCATT 
GAAGTTATTG TATGGGATGG CCCTGATAAT GAGCCTGTGG TGTATGTTGG CAGTTCTGTC 
GCCTCACATC TGGTTTTCTC CAAAATTCTC GACATTATAA ACCAGTATGC TTTCGAGAGC 
TCCGAGTACC CCCTGATTCT CTGCTTGGTG ACCCACTGCT GCATCCTCCA GCAGAGGGTC 
ATGGCACAAC ACATGAAGAA GATTTTTGGG GACAAGCTGT ATGTTGATCC TCCAAACAAG 
GAGGAAAACT ATCTTCTCTC CCCAGAGAAA CTCAAAGGCA AAATCCTCCT CAAGGGTAAA 
AAACTACCAC CCAGCTGTAC CGATGCTGAG GGGGATGTAA CAGATGAGGA AGAAGGTCTG 
GAAATGTCTC GGAGATTAGG ATCTGATGAA AAGGACCAAC TAAATGGACT CGGCTACAAG 
AGACTTAGAT TGTGCCGGGA ACTGTCTGAG CTTGTGTCTC TCTGCAAGTC AGTCCAGTTC 
AGAGACTTTG AAATCACAAA GAGGGAGCAA AAATACTGGG AGATGTGCTC CTTCAATGAG 
GTGGATGCAA ACAGATATGC CAATGAGTTC CCAGAGGAAT TTGTTTGTTA TAACAAGCGC 
TTTCTCTCCA GAGTGTATCC CACACCTATG AGGATTGATG CCAGCAACAT GAACCCCCAG 
GATTTCTGGA AGTGTGGCTG CCAAATAGTG GCCATGAACT ACCAGACGCC AGGTCTGATG 
ATGGACCTCA ACCTAGGTTG GTTCAGACAG AATGGAAACT GTGGCTATGT TTTACGTCCT 
GCAATTATGA GAGAGGAGGT GTCCTACTTC AGTGCCAATG CTCGAGATTC CCTGCCAGGA 
GTGTCTGCCC AGCTTCTCCA CATAAAAGTC ATCAGCGGAC AGAACCTGCC CAAGCCTCGA 
GGATCTGCCG CCAAGGGTGA TGTGGTGGAG CCCTACATTT ATGTGGAGAT CCACGGTATC 
CCAGCTGACT GTGCTGAGCA GAGAACCAAA ACTGTCTCTC AGAATGGCGA CAACCCTATT 
TTCGACGAGA GTTTTGAATT TCAGATTAAT TTACCAGAAC TCGCCGTGCT GCGCTTTGTG 
GTGCTGGACG ACGATTACAT CGGAGATGAG TTCATCGGCC AGTACACCAT ACCGTTTGAG 
TGCCTACAGC CCGGCTATAG ACATGTCCCG CTGCAGTCTC TGACTGGAGA GTTCCTACCA 
AACACCACCT TGTTTGTTCA CGTGGCCATC ACCAACAGAC GTGGTGGAGG AAAGGCTCAT 
AAGAGGGGTC TGTCTGTGAG GAAGGGGAGG AAGACCCGGG AATACACCAC CACCAAGTCC 
ACGGGGATCA AGATAGTGGA TGAGCTCTTC AGAGCTTCCA CTCAGCCCCT CAGGGAAGCC 
ACAGACCTCA GAGAGAATGT GCAGAATGCT GTGGTTTCCT TCAAGGAGCT GTGTGGCCTG 
ACTCCAGCGG CCAACATGAA GCAGTGTATC CTGACCGTGT CTACCTGGCT GATGAACAGT 
GAGCGGTCCC TGAGGGTAAC CGTGGACCTG AGCGAGACTT ACCCCACCAT GGAAGCTCAA 
GGTCCTGTCC CTGAACTGCT GCGGAAAGTG CTCAATGCTT ACGACATGAT GATCCAAACC 
AGCAGAACAC TGATCGAGTC AGCTGATGTC ATCTACTCCA AGCTCACACA AGCCCAGCGA 
GCAGGTTTGG ACTTCCACGA GGACCTGCAC CGGATTGGCG CCAAGGAGGG CCTGAAGGGG 
CGTAAACTTC AAAAGGCCAT GGAGAGCTAT GCTTGGAACA TCACTGTCCT CAAGGGCCAA 
GCGGACCTGC TGAAGCACGC TAAGAGCGAG GCGCTGGACA CACTGCGTCA GATCCACTGT 
GCAGCTCAGT CCTGCGGTCT CAGTAAGAAT GAAGCGGCTT CCCCACAGGA GCAACACCAT 
CCTCAGCAGC AGCCCCAGCA GCTCCAGCAG CTCCCGCAGC TCCAGATTCA GCAACAGTCT 
ACGGCTAAGG CACTGTCACA GCCCACCTCG CTGTACCTGG CTCAACCACA GACTACACCT 
CAGGCTTATA CCCTTCCTCT TCCTCAGCAA CATCCTCTGT TACCAGCTCA CACTCTGGTT 
CAGACTGTTC TAAAACCACC AGCGTATTCT CACCCACCAC CCCTGCCCCA GGGTCCGTTA 
CCACCCCAAG GGCCACAGCA GAGGGCAGCG GGTCCTTTGG ACGTCATCCC CCAGAGAGAG 
GTGGCCAGTG ACAGTCCTCC AGGATCCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101071575 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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