LOC101068515 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101068515
Entrez Gene ID	101068515
Full Name	collagen alpha-1(XXVIII) chain-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011609955.2	XP_011608257.2	collagen alpha-1(XXVIII) chain-like isoform X1
XM_011609955.1	XP_011608257.1	collagen alpha-1(XXVIII) chain-like isoform X1
XM_011609969.2	XP_011608271.2	collagen alpha-1(XXVIII) chain-like isoform X2
XM_011609959.1	XP_011608261.1	collagen alpha-1(XXVIII) chain-like isoform X1
XM_011609959.2	XP_011608261.2	collagen alpha-1(XXVIII) chain-like isoform X1
XM_011609969.1	XP_011608271.1	collagen alpha-1(XXVIII) chain-like isoform X2
XM_011609975.1	XP_011608277.1	collagen alpha-1(XXVIII) chain-like isoform X3
XM_011609975.2	XP_011608277.2	collagen alpha-1(XXVIII) chain-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC101068515 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101068515 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo24124	XM_011609955.1 Latest version!	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo24124	XM_011609959.1 Latest version!	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo24125	XM_011609969.1 Latest version!	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo24126	XM_011609975.1 Latest version!	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo43047	XM_011609955.2 Latest version!	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo43047	XM_011609959.2 Latest version!	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo43048	XM_011609969.2 Latest version!	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo43049	XM_011609975.2 Latest version!	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101068515 ( XM_011609955.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011608257.1 Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo24124
Clone ID Related Accession (Same CDS sequence)	XM_011609955.1 , XM_011609959.1
Accession Version	XM_011609955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-1(XXVIII) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAG 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTTGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGGCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATGT TGGGTCTTCT GGTCCCCCTG GTCCAATTGG GGAGACAGGC 
CATGGATTAC CTGGTCCAAA GGGTGATCAT GGTCATCCAG GTTTACCAGG ACCATATGGT 
CCCAAAGGTG AAGGTCTTCC TGGCCCTATG GGTCCTACGG GTTTGCCAGG TTTACCAGGT 
GAGCCCGGTC CTGAAGGAAT TGGAGTTCCT GGTCCGAAGG GTGATATTGG CTTCAGAGGA 
TTGCCAGGAT TGCCTGGACC ACCTGGGGAG GGCATTCAAG GACCACCTGG TAATATTGGA 
AGGCCAGGGC CTCCTGGTCC AAGGGGACCA CAAGGAGATG GTATTCAAGG CCCAAAGGGT 
GAGCCAGGAT CTCAGGGTAT GACAGGTCCC AGAGGGCCGC CGGGAGATGG ACTCCCTGGA 
GCTAAGGGTG ATCGGGGATT AACTGGTGAA AAGGGGGGAA AAGGTGGAAA AGGAGACCTG 
GGAGATTCTG GAGTGCCTGG TGAAGCAGGA AGGGCGGGAG CAAAAGGAGA TCCAGGCCTC 
ACCAGAGAGG ACATCATTAA GCTCATTAAA GAAATCTGTG GATGTGGCAT CAAATGCAAA 
GAAAGACCGA TGGAGCTGGT GTTCGTCATT GACAGCTCAG AGAGTGTTGG TCCAGAGAAT 
TTTGAAATCA TCAAGGACTT TGTCATCAGG CTGGTGGACC GTACCACAGT TGGACGTAAC 
GCCACCAGAA TTGGACTAGT CCTCTACAGT CTGGATGTCC ATTTAGAGTT CAACTTGGCT 
CGTTACGCGA CCAAGCAGGA CATCAGGCAC GCCATCAGAA AAATCCCTTA TATGGGCGAG 
GGCACCTACA CTGGCACGGC AATCAGGAAG GCAACTCAGG AGGCTTTCTT AAACGCTCGG 
CGTGGCGTCA GTAAAGTGGC TATTGTCATC ACAGATGGTC AGACAGACAA ACGAGAGCCA 
GTCAAGCTGG ACCTGGCTGT GAGAGAAGCC CACGCTGCAA ACATTGAGAT GTATGCCCTG 
GGCATTGTCA ACGCTTCGGA TCCCACACAG GCCGAGTTCC TGCAGGAACT CAACCTCATC 
GCCTCTGACC CTGACGATGA ACACATGTAC CTCATCGATG ACTTCAATAC TCTACCAGCA 
CTGGAATCTA AATTGGTCAG CCAGTTCTGT GAGGATGAAA ATGGAGCCCT GATTTACAAC 
CACATAACAA ATGGACACTG GAACGGCAAC AGTGGACATG GGGACAGTGT TGGTGACAAT 
AATGGCCAGA ACAGTAACAG CAACAGAGGA TTTGGAAAGA GCGGGATCAG TTCCAGCGAT 
AAAGAGGAGA TCCAAAATCG GAGACGCCAC AACAGTCGAA GCCGTGGAGA TACTTTCACA 
CTGCCCATAA GTGCCGATCC TCTTCCTGTG CAGGTGGAGG AGGATGATGA AGGTGAAGAT 
TTAGACATTA GAGCCCAAGC CCGCATCAGT AGTATTGCAA CATTAATTAA CAAAACATCT 
GTAGTATCTG TCAGAGAAGG CTCCATCTCT AATGATGTGG TTTTATCATC TTCATCGTCT 
TCATCTAACA CTTCAACCTC ATCTGCATCA ACTTTATCTT TAAACTCTAA CAAGTTACAG 
TCAACAGTCC TACCACAAGA GGTTTCTCCC CTTGATCCAC GCTGTAGTCT GAGTTTGAAT 
CAAGGTCCCT GTCGAAACTA CACCATCCAC TGGTACTATG ACAAGCAGGC CAACTCCTGC 
GCACAGTTTT GGTACGGAGG CTGTGGAGGA AATGATAATC GTTATGAAAC GGAGGAAGAA 
TGCAAGAAAA CCTGTGTAGT AAGCCTTCGC AGAGAAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011608257.1
CDS	173..3814
Translation	MLSCTWVLLVTGLSCVWTQQTLEEKKPVNKVLSAGTHGRQAQLHEECGLEISFLLDSSESAKDNHEQEKQFVMKVVDRLQGKKLHTGRSLNLRVALLQYSSHVITEQTFKDWRGTENFKARVTPIIYIGHGTYTTYAITNMTKIYREESSPGSIRVAVLLTDGISHPRNPDIFSAVAEAKNQGIKFFTLGITRAANEPTNIAQLRLVSSSPASRFLHNLQDEDIVEKIVAEITSLADQGCPLAQKCACEKGERGLSGPAGKKGRPGEDGAPGVKGEKGEYGISGLPGQEGPEGKPGYKGEKGERGECGTPGVKGDAGPVGLVGTRGPRGLQGLPGPPGDIGPEGIQGKQGERGPTGPPGIQGETGIGRPGPKGDIGFQGQPGPPGPRGIGELGPPGPQGPQGVQGSKGPTGEGLPGPKGDRGLPGPRGPRGQQGVGIKGDKGDFGPPGFPGPTGPTGVGIQGEKGVEGPRGPPGVRGLPGEGLPGPKGDQGLPGEQGAPGDRGIGEAGQKGEPGSAGIGGLPGLPGEDGAPGQKGEPGLPGLRGTEGAQGIGTQGEKGDQGQRGIRGLHGPPGIPGPSGPKGERGIPGQQGVQGQPGRSIPGPKGDVGSSGPPGPIGETGHGLPGPKGDHGHPGLPGPYGPKGEGLPGPMGPTGLPGLPGEPGPEGIGVPGPKGDIGFRGLPGLPGPPGEGIQGPPGNIGRPGPPGPRGPQGDGIQGPKGEPGSQGMTGPRGPPGDGLPGAKGDRGLTGEKGGKGGKGDLGDSGVPGEAGRAGAKGDPGLTREDIIKLIKEICGCGIKCKERPMELVFVIDSSESVGPENFEIIKDFVIRLVDRTTVGRNATRIGLVLYSLDVHLEFNLARYATKQDIRHAIRKIPYMGEGTYTGTAIRKATQEAFLNARRGVSKVAIVITDGQTDKREPVKLDLAVREAHAANIEMYALGIVNASDPTQAEFLQELNLIASDPDDEHMYLIDDFNTLPALESKLVSQFCEDENGALIYNHITNGHWNGNSGHGDSVGDNNGQNSNSNRGFGKSGISSSDKEEIQNRRRHNSRSRGDTFTLPISADPLPVQVEEDDEGEDLDIRAQARISSIATLINKTSVVSVREGSISNDVVLSSSSSSSNTSTSSASTLSLNSNKLQSTVLPQEVSPLDPRCSLSLNQGPCRNYTIHWYYDKQANSCAQFWYGGCGGNDNRYETEEECKKTCVVSLRREG

Target ORF information:

RefSeq Version	XM_011609955.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609955.1

ORF Insert Sequence:

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAG 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTTGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGGCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATGT TGGGTCTTCT GGTCCCCCTG GTCCAATTGG GGAGACAGGC 
CATGGATTAC CTGGTCCAAA GGGTGATCAT GGTCATCCAG GTTTACCAGG ACCATATGGT 
CCCAAAGGTG AAGGTCTTCC TGGCCCTATG GGTCCTACGG GTTTGCCAGG TTTACCAGGT 
GAGCCCGGTC CTGAAGGAAT TGGAGTTCCT GGTCCGAAGG GTGATATTGG CTTCAGAGGA 
TTGCCAGGAT TGCCTGGACC ACCTGGGGAG GGCATTCAAG GACCACCTGG TAATATTGGA 
AGGCCAGGGC CTCCTGGTCC AAGGGGACCA CAAGGAGATG GTATTCAAGG CCCAAAGGGT 
GAGCCAGGAT CTCAGGGTAT GACAGGTCCC AGAGGGCCGC CGGGAGATGG ACTCCCTGGA 
GCTAAGGGTG ATCGGGGATT AACTGGTGAA AAGGGGGGAA AAGGTGGAAA AGGAGACCTG 
GGAGATTCTG GAGTGCCTGG TGAAGCAGGA AGGGCGGGAG CAAAAGGAGA TCCAGGCCTC 
ACCAGAGAGG ACATCATTAA GCTCATTAAA GAAATCTGTG GATGTGGCAT CAAATGCAAA 
GAAAGACCGA TGGAGCTGGT GTTCGTCATT GACAGCTCAG AGAGTGTTGG TCCAGAGAAT 
TTTGAAATCA TCAAGGACTT TGTCATCAGG CTGGTGGACC GTACCACAGT TGGACGTAAC 
GCCACCAGAA TTGGACTAGT CCTCTACAGT CTGGATGTCC ATTTAGAGTT CAACTTGGCT 
CGTTACGCGA CCAAGCAGGA CATCAGGCAC GCCATCAGAA AAATCCCTTA TATGGGCGAG 
GGCACCTACA CTGGCACGGC AATCAGGAAG GCAACTCAGG AGGCTTTCTT AAACGCTCGG 
CGTGGCGTCA GTAAAGTGGC TATTGTCATC ACAGATGGTC AGACAGACAA ACGAGAGCCA 
GTCAAGCTGG ACCTGGCTGT GAGAGAAGCC CACGCTGCAA ACATTGAGAT GTATGCCCTG 
GGCATTGTCA ACGCTTCGGA TCCCACACAG GCCGAGTTCC TGCAGGAACT CAACCTCATC 
GCCTCTGACC CTGACGATGA ACACATGTAC CTCATCGATG ACTTCAATAC TCTACCAGCA 
CTGGAATCTA AATTGGTCAG CCAGTTCTGT GAGGATGAAA ATGGAGCCCT GATTTACAAC 
CACATAACAA ATGGACACTG GAACGGCAAC AGTGGACATG GGGACAGTGT TGGTGACAAT 
AATGGCCAGA ACAGTAACAG CAACAGAGGA TTTGGAAAGA GCGGGATCAG TTCCAGCGAT 
AAAGAGGAGA TCCAAAATCG GAGACGCCAC AACAGTCGAA GCCGTGGAGA TACTTTCACA 
CTGCCCATAA GTGCCGATCC TCTTCCTGTG CAGGTGGAGG AGGATGATGA AGGTGAAGAT 
TTAGACATTA GAGCCCAAGC CCGCATCAGT AGTATTGCAA CATTAATTAA CAAAACATCT 
GTAGTATCTG TCAGAGAAGG CTCCATCTCT AATGATGTGG TTTTATCATC TTCATCGTCT 
TCATCTAACA CTTCAACCTC ATCTGCATCA ACTTTATCTT TAAACTCTAA CAAGTTACAG 
TCAACAGTCC TACCACAAGA GGTTTCTCCC CTTGATCCAC GCTGTAGTCT GAGTTTGAAT 
CAAGGTCCCT GTCGAAACTA CACCATCCAC TGGTACTATG ACAAGCAGGC CAACTCCTGC 
GCACAGTTTT GGTACGGAGG CTGTGGAGGA AATGATAATC GTTATGAAAC GGAGGAAGAA 
TGCAAGAAAA CCTGTGTAGT AAGCCTTCGC AGAGAAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101068515 ( XM_011609959.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011608261.1 Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo24124
Clone ID Related Accession (Same CDS sequence)	XM_011609955.1 , XM_011609959.1
Accession Version	XM_011609959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-1(XXVIII) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAG 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTTGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGGCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATGT TGGGTCTTCT GGTCCCCCTG GTCCAATTGG GGAGACAGGC 
CATGGATTAC CTGGTCCAAA GGGTGATCAT GGTCATCCAG GTTTACCAGG ACCATATGGT 
CCCAAAGGTG AAGGTCTTCC TGGCCCTATG GGTCCTACGG GTTTGCCAGG TTTACCAGGT 
GAGCCCGGTC CTGAAGGAAT TGGAGTTCCT GGTCCGAAGG GTGATATTGG CTTCAGAGGA 
TTGCCAGGAT TGCCTGGACC ACCTGGGGAG GGCATTCAAG GACCACCTGG TAATATTGGA 
AGGCCAGGGC CTCCTGGTCC AAGGGGACCA CAAGGAGATG GTATTCAAGG CCCAAAGGGT 
GAGCCAGGAT CTCAGGGTAT GACAGGTCCC AGAGGGCCGC CGGGAGATGG ACTCCCTGGA 
GCTAAGGGTG ATCGGGGATT AACTGGTGAA AAGGGGGGAA AAGGTGGAAA AGGAGACCTG 
GGAGATTCTG GAGTGCCTGG TGAAGCAGGA AGGGCGGGAG CAAAAGGAGA TCCAGGCCTC 
ACCAGAGAGG ACATCATTAA GCTCATTAAA GAAATCTGTG GATGTGGCAT CAAATGCAAA 
GAAAGACCGA TGGAGCTGGT GTTCGTCATT GACAGCTCAG AGAGTGTTGG TCCAGAGAAT 
TTTGAAATCA TCAAGGACTT TGTCATCAGG CTGGTGGACC GTACCACAGT TGGACGTAAC 
GCCACCAGAA TTGGACTAGT CCTCTACAGT CTGGATGTCC ATTTAGAGTT CAACTTGGCT 
CGTTACGCGA CCAAGCAGGA CATCAGGCAC GCCATCAGAA AAATCCCTTA TATGGGCGAG 
GGCACCTACA CTGGCACGGC AATCAGGAAG GCAACTCAGG AGGCTTTCTT AAACGCTCGG 
CGTGGCGTCA GTAAAGTGGC TATTGTCATC ACAGATGGTC AGACAGACAA ACGAGAGCCA 
GTCAAGCTGG ACCTGGCTGT GAGAGAAGCC CACGCTGCAA ACATTGAGAT GTATGCCCTG 
GGCATTGTCA ACGCTTCGGA TCCCACACAG GCCGAGTTCC TGCAGGAACT CAACCTCATC 
GCCTCTGACC CTGACGATGA ACACATGTAC CTCATCGATG ACTTCAATAC TCTACCAGCA 
CTGGAATCTA AATTGGTCAG CCAGTTCTGT GAGGATGAAA ATGGAGCCCT GATTTACAAC 
CACATAACAA ATGGACACTG GAACGGCAAC AGTGGACATG GGGACAGTGT TGGTGACAAT 
AATGGCCAGA ACAGTAACAG CAACAGAGGA TTTGGAAAGA GCGGGATCAG TTCCAGCGAT 
AAAGAGGAGA TCCAAAATCG GAGACGCCAC AACAGTCGAA GCCGTGGAGA TACTTTCACA 
CTGCCCATAA GTGCCGATCC TCTTCCTGTG CAGGTGGAGG AGGATGATGA AGGTGAAGAT 
TTAGACATTA GAGCCCAAGC CCGCATCAGT AGTATTGCAA CATTAATTAA CAAAACATCT 
GTAGTATCTG TCAGAGAAGG CTCCATCTCT AATGATGTGG TTTTATCATC TTCATCGTCT 
TCATCTAACA CTTCAACCTC ATCTGCATCA ACTTTATCTT TAAACTCTAA CAAGTTACAG 
TCAACAGTCC TACCACAAGA GGTTTCTCCC CTTGATCCAC GCTGTAGTCT GAGTTTGAAT 
CAAGGTCCCT GTCGAAACTA CACCATCCAC TGGTACTATG ACAAGCAGGC CAACTCCTGC 
GCACAGTTTT GGTACGGAGG CTGTGGAGGA AATGATAATC GTTATGAAAC GGAGGAAGAA 
TGCAAGAAAA CCTGTGTAGT AAGCCTTCGC AGAGAAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011608261.1
CDS	181..3822
Translation	MLSCTWVLLVTGLSCVWTQQTLEEKKPVNKVLSAGTHGRQAQLHEECGLEISFLLDSSESAKDNHEQEKQFVMKVVDRLQGKKLHTGRSLNLRVALLQYSSHVITEQTFKDWRGTENFKARVTPIIYIGHGTYTTYAITNMTKIYREESSPGSIRVAVLLTDGISHPRNPDIFSAVAEAKNQGIKFFTLGITRAANEPTNIAQLRLVSSSPASRFLHNLQDEDIVEKIVAEITSLADQGCPLAQKCACEKGERGLSGPAGKKGRPGEDGAPGVKGEKGEYGISGLPGQEGPEGKPGYKGEKGERGECGTPGVKGDAGPVGLVGTRGPRGLQGLPGPPGDIGPEGIQGKQGERGPTGPPGIQGETGIGRPGPKGDIGFQGQPGPPGPRGIGELGPPGPQGPQGVQGSKGPTGEGLPGPKGDRGLPGPRGPRGQQGVGIKGDKGDFGPPGFPGPTGPTGVGIQGEKGVEGPRGPPGVRGLPGEGLPGPKGDQGLPGEQGAPGDRGIGEAGQKGEPGSAGIGGLPGLPGEDGAPGQKGEPGLPGLRGTEGAQGIGTQGEKGDQGQRGIRGLHGPPGIPGPSGPKGERGIPGQQGVQGQPGRSIPGPKGDVGSSGPPGPIGETGHGLPGPKGDHGHPGLPGPYGPKGEGLPGPMGPTGLPGLPGEPGPEGIGVPGPKGDIGFRGLPGLPGPPGEGIQGPPGNIGRPGPPGPRGPQGDGIQGPKGEPGSQGMTGPRGPPGDGLPGAKGDRGLTGEKGGKGGKGDLGDSGVPGEAGRAGAKGDPGLTREDIIKLIKEICGCGIKCKERPMELVFVIDSSESVGPENFEIIKDFVIRLVDRTTVGRNATRIGLVLYSLDVHLEFNLARYATKQDIRHAIRKIPYMGEGTYTGTAIRKATQEAFLNARRGVSKVAIVITDGQTDKREPVKLDLAVREAHAANIEMYALGIVNASDPTQAEFLQELNLIASDPDDEHMYLIDDFNTLPALESKLVSQFCEDENGALIYNHITNGHWNGNSGHGDSVGDNNGQNSNSNRGFGKSGISSSDKEEIQNRRRHNSRSRGDTFTLPISADPLPVQVEEDDEGEDLDIRAQARISSIATLINKTSVVSVREGSISNDVVLSSSSSSSNTSTSSASTLSLNSNKLQSTVLPQEVSPLDPRCSLSLNQGPCRNYTIHWYYDKQANSCAQFWYGGCGGNDNRYETEEECKKTCVVSLRREG

Target ORF information:

RefSeq Version	XM_011609959.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609959.1

ORF Insert Sequence:

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAG 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTTGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGGCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATGT TGGGTCTTCT GGTCCCCCTG GTCCAATTGG GGAGACAGGC 
CATGGATTAC CTGGTCCAAA GGGTGATCAT GGTCATCCAG GTTTACCAGG ACCATATGGT 
CCCAAAGGTG AAGGTCTTCC TGGCCCTATG GGTCCTACGG GTTTGCCAGG TTTACCAGGT 
GAGCCCGGTC CTGAAGGAAT TGGAGTTCCT GGTCCGAAGG GTGATATTGG CTTCAGAGGA 
TTGCCAGGAT TGCCTGGACC ACCTGGGGAG GGCATTCAAG GACCACCTGG TAATATTGGA 
AGGCCAGGGC CTCCTGGTCC AAGGGGACCA CAAGGAGATG GTATTCAAGG CCCAAAGGGT 
GAGCCAGGAT CTCAGGGTAT GACAGGTCCC AGAGGGCCGC CGGGAGATGG ACTCCCTGGA 
GCTAAGGGTG ATCGGGGATT AACTGGTGAA AAGGGGGGAA AAGGTGGAAA AGGAGACCTG 
GGAGATTCTG GAGTGCCTGG TGAAGCAGGA AGGGCGGGAG CAAAAGGAGA TCCAGGCCTC 
ACCAGAGAGG ACATCATTAA GCTCATTAAA GAAATCTGTG GATGTGGCAT CAAATGCAAA 
GAAAGACCGA TGGAGCTGGT GTTCGTCATT GACAGCTCAG AGAGTGTTGG TCCAGAGAAT 
TTTGAAATCA TCAAGGACTT TGTCATCAGG CTGGTGGACC GTACCACAGT TGGACGTAAC 
GCCACCAGAA TTGGACTAGT CCTCTACAGT CTGGATGTCC ATTTAGAGTT CAACTTGGCT 
CGTTACGCGA CCAAGCAGGA CATCAGGCAC GCCATCAGAA AAATCCCTTA TATGGGCGAG 
GGCACCTACA CTGGCACGGC AATCAGGAAG GCAACTCAGG AGGCTTTCTT AAACGCTCGG 
CGTGGCGTCA GTAAAGTGGC TATTGTCATC ACAGATGGTC AGACAGACAA ACGAGAGCCA 
GTCAAGCTGG ACCTGGCTGT GAGAGAAGCC CACGCTGCAA ACATTGAGAT GTATGCCCTG 
GGCATTGTCA ACGCTTCGGA TCCCACACAG GCCGAGTTCC TGCAGGAACT CAACCTCATC 
GCCTCTGACC CTGACGATGA ACACATGTAC CTCATCGATG ACTTCAATAC TCTACCAGCA 
CTGGAATCTA AATTGGTCAG CCAGTTCTGT GAGGATGAAA ATGGAGCCCT GATTTACAAC 
CACATAACAA ATGGACACTG GAACGGCAAC AGTGGACATG GGGACAGTGT TGGTGACAAT 
AATGGCCAGA ACAGTAACAG CAACAGAGGA TTTGGAAAGA GCGGGATCAG TTCCAGCGAT 
AAAGAGGAGA TCCAAAATCG GAGACGCCAC AACAGTCGAA GCCGTGGAGA TACTTTCACA 
CTGCCCATAA GTGCCGATCC TCTTCCTGTG CAGGTGGAGG AGGATGATGA AGGTGAAGAT 
TTAGACATTA GAGCCCAAGC CCGCATCAGT AGTATTGCAA CATTAATTAA CAAAACATCT 
GTAGTATCTG TCAGAGAAGG CTCCATCTCT AATGATGTGG TTTTATCATC TTCATCGTCT 
TCATCTAACA CTTCAACCTC ATCTGCATCA ACTTTATCTT TAAACTCTAA CAAGTTACAG 
TCAACAGTCC TACCACAAGA GGTTTCTCCC CTTGATCCAC GCTGTAGTCT GAGTTTGAAT 
CAAGGTCCCT GTCGAAACTA CACCATCCAC TGGTACTATG ACAAGCAGGC CAACTCCTGC 
GCACAGTTTT GGTACGGAGG CTGTGGAGGA AATGATAATC GTTATGAAAC GGAGGAAGAA 
TGCAAGAAAA CCTGTGTAGT AAGCCTTCGC AGAGAAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101068515 ( XM_011609969.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011608271.1 Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X3, mRNA.

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CloneID	OTo24125
Clone ID Related Accession (Same CDS sequence)	XM_011609969.1
Accession Version	XM_011609969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-1(XXVIII) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAG 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGCTCAGCTA 
CATGAGGAGT GTGGTCTTGA GATCTCCTTC CTGTTGGACA GCTCTGAGAG CGCAAAGGAC 
AACCATGAGC AGGAGAAACA GTTTGTCATG AAGGTGGTGG ACAGACTCCA GGGGAAAAAG 
CTGCACACAG GCCGCAGCCT GAACCTGAGG GTGGCTCTGT TGCAGTACAG CAGTCACGTC 
ATCACGGAGC AAACCTTTAA GGACTGGAGG GGCACGGAGA ACTTCAAGGC TCGTGTAACT 
CCCATCATCT ACATCGGGCA CGGCACCTAC ACCACCTACG CCATCACCAA CATGACCAAG 
ATCTACCGGG AGGAGTCCAG TCCGGGCAGC ATCAGAGTGG CCGTGCTGCT CACAGATGGC 
ATTTCCCACC CGAGGAATCC GGATATTTTC TCTGCTGTGG CGGAAGCGAA AAACCAGGGC 
ATCAAATTCT TCACCCTGGG CATCACCCGC GCAGCCAACG AGCCCACCAA CATAGCCCAG 
CTCCGTCTTG TCTCCAGCTC CCCGGCCTCC CGCTTCCTCC ATAACCTGCA GGATGAAGAC 
ATTGTGGAGA AAATAGTTGC GGAGATTACC AGTTTGGCTG ATCAAGGATG CCCTTTGGCT 
CAGAAATGTG CTTGTGAGAA AGGAGAACGG GGCCTAAGTG GGCCTGCGGG CAAAAAAGGT 
CGGCCTGGAG AGGACGGAGC TCCAGGTGTT AAAGGGGAAA AGGGAGAATA TGGAATAAGT 
GGTCTACCTG GACAAGAGGG TCCTGAGGGT AAACCAGGTT ACAAGGGAGA GAAGGGAGAG 
AGAGGTGAGT GTGGTACTCC AGGAGTAAAA GGAGACGCGG GTCCTGTTGG TTTGGTCGGA 
ACGAGGGGAC CTCGGGGTTT GCAGGGGTTA CCAGGACCAC CAGGGGACAT TGGACCTGAG 
GGAATTCAGG GAAAGCAGGG CGAACGTGGC CCAACTGGCC CTCCAGGAAT TCAGGGGGAA 
ACTGGAATTG GACGTCCTGG TCCAAAGGGT GACATTGGAT TCCAAGGTCA GCCCGGTCCC 
CCCGGTCCTC GTGGAATAGG TGAACTAGGT CCTCCTGGTC CTCAAGGGCC GCAAGGTGTC 
CAAGGCAGTA AAGGACCCAC TGGTGAAGGT CTGCCTGGAC CAAAGGGGGA TCGGGGGCTC 
CCTGGACCGA GGGGGCCGAG GGGACAACAA GGAGTGGGAA TTAAAGGAGA TAAGGGGGAC 
TTTGGGCCTC CTGGTTTTCC AGGGCCAACT GGGCCAACAG GAGTTGGGAT ACAAGGAGAA 
AAGGGAGTTG AGGGTCCAAG AGGGCCACCA GGAGTCAGAG GACTTCCAGG CGAAGGTCTT 
CCTGGGCCTA AGGGAGATCA AGGTTTACCA GGAGAGCAAG GAGCTCCGGG AGATAGAGGT 
ATTGGTGAAG CCGGTCAAAA GGGAGAACCT GGGTCAGCTG GAATCGGAGG CCTCCCTGGT 
CTTCCAGGAG AGGATGGAGC ACCAGGGCAG AAGGGGGAGC CTGGTTTGCC TGGCTTAAGG 
GGAACCGAGG GAGCTCAAGG AATTGGTACT CAAGGGGAAA AGGGTGACCA GGGTCAAAGG 
GGCATTCGTG GATTACATGG GCCTCCTGGA ATCCCAGGAC CCTCTGGTCC AAAGGGTGAA 
CGTGGGATAC CAGGCCAGCA GGGCGTGCAG GGACAGCCAG GAAGGTCCAT ACCTGGTCCA 
AAGGGTGATG TTGGGTCTTC TGGTCCCCCT GGTCCAATTG GGGAGACAGG CCATGGATTA 
CCTGGTCCAA AGGGTGATCA TGGTCATCCA GGTTTACCAG GACCATATGG TCCCAAAGGT 
GAAGGTCTTC CTGGCCCTAT GGGTCCTACG GGTTTGCCAG GTTTACCAGG TGAGCCCGGT 
CCTGAAGGAA TTGGAGTTCC TGGTCCGAAG GGTGATATTG GCTTCAGAGG ATTGCCAGGA 
TTGCCTGGAC CACCTGGGGA GGGCATTCAA GGACCACCTG GTAATATTGG AAGGCCAGGG 
CCTCCTGGTC CAAGGGGACC ACAAGGAGAT GGTATTCAAG GCCCAAAGGG TGAGCCAGGA 
TCTCAGGGTA TGACAGGTCC CAGAGGGCCG CCGGGAGATG GACTCCCTGG AGCTAAGGGT 
GATCGGGGAT TAACTGGTGA AAAGGGGGGA AAAGGTGGAA AAGGAGACCT GGGAGATTCT 
GGAGTGCCTG GTGAAGCAGG AAGGGCGGGA GCAAAAGGAG ATCCAGGCCT CACCAGAGAG 
GACATCATTA AGCTCATTAA AGAAATCTGT GGATGTGGCA TCAAATGCAA AGAAAGACCG 
ATGGAGCTGG TGTTCGTCAT TGACAGCTCA GAGAGTGTTG GTCCAGAGAA TTTTGAAATC 
ATCAAGGACT TTGTCATCAG GCTGGTGGAC CGTACCACAG TTGGACGTAA CGCCACCAGA 
ATTGGACTAG TCCTCTACAG TCTGGATGTC CATTTAGAGT TCAACTTGGC TCGTTACGCG 
ACCAAGCAGG ACATCAGGCA CGCCATCAGA AAAATCCCTT ATATGGGCGA GGGCACCTAC 
ACTGGCACGG CAATCAGGAA GGCAACTCAG GAGGCTTTCT TAAACGCTCG GCGTGGCGTC 
AGTAAAGTGG CTATTGTCAT CACAGATGGT CAGACAGACA AACGAGAGCC AGTCAAGCTG 
GACCTGGCTG TGAGAGAAGC CCACGCTGCA AACATTGAGA TGTATGCCCT GGGCATTGTC 
AACGCTTCGG ATCCCACACA GGCCGAGTTC CTGCAGGAAC TCAACCTCAT CGCCTCTGAC 
CCTGACGATG AACACATGTA CCTCATCGAT GACTTCAATA CTCTACCAGC ACTGGAATCT 
AAATTGGTCA GCCAGTTCTG TGAGGATGAA AATGGAGCCC TGATTTACAA CCACATAACA 
AATGGACACT GGAACGGCAA CAGTGGACAT GGGGACAGTG TTGGTGACAA TAATGGCCAG 
AACAGTAACA GCAACAGAGG ATTTGGAAAG AGCGGGATCA GTTCCAGCGA TAAAGAGGAG 
ATCCAAAATC GGAGACGCCA CAACAGTCGA AGCCGTGGAG ATACTTTCAC ACTGCCCATA 
AGTGCCGATC CTCTTCCTGT GCAGGTGGAG GAGGATGATG AAGGTGAAGA TTTAGACATT 
AGAGCCCAAG CCCGCATCAG TAGTATTGCA ACATTAATTA ACAAAACATC TGTAGTATCT 
GTCAGAGAAG GCTCCATCTC TAATGATGTG GTTTTATCAT CTTCATCGTC TTCATCTAAC 
ACTTCAACCT CATCTGCATC AACTTTATCT TTAAACTCTA ACAAGTTACA GTCAACAGTC 
CTACCACAAG AGGTTTCTCC CCTTGATCCA CGCTGTAGTC TGAGTTTGAA TCAAGGTCCC 
TGTCGAAACT ACACCATCCA CTGGTACTAT GACAAGCAGG CCAACTCCTG CGCACAGTTT 
TGGTACGGAG GCTGTGGAGG AAATGATAAT CGTTATGAAA CGGAGGAAGA ATGCAAGAAA 
ACCTGTGTAG TAAGCCTTCG CAGAGAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011608271.1
CDS	173..3805
Translation	MLSCTWVLLVTGLSCVWTQQTLEEKKPVNKVLSAGTHAQLHEECGLEISFLLDSSESAKDNHEQEKQFVMKVVDRLQGKKLHTGRSLNLRVALLQYSSHVITEQTFKDWRGTENFKARVTPIIYIGHGTYTTYAITNMTKIYREESSPGSIRVAVLLTDGISHPRNPDIFSAVAEAKNQGIKFFTLGITRAANEPTNIAQLRLVSSSPASRFLHNLQDEDIVEKIVAEITSLADQGCPLAQKCACEKGERGLSGPAGKKGRPGEDGAPGVKGEKGEYGISGLPGQEGPEGKPGYKGEKGERGECGTPGVKGDAGPVGLVGTRGPRGLQGLPGPPGDIGPEGIQGKQGERGPTGPPGIQGETGIGRPGPKGDIGFQGQPGPPGPRGIGELGPPGPQGPQGVQGSKGPTGEGLPGPKGDRGLPGPRGPRGQQGVGIKGDKGDFGPPGFPGPTGPTGVGIQGEKGVEGPRGPPGVRGLPGEGLPGPKGDQGLPGEQGAPGDRGIGEAGQKGEPGSAGIGGLPGLPGEDGAPGQKGEPGLPGLRGTEGAQGIGTQGEKGDQGQRGIRGLHGPPGIPGPSGPKGERGIPGQQGVQGQPGRSIPGPKGDVGSSGPPGPIGETGHGLPGPKGDHGHPGLPGPYGPKGEGLPGPMGPTGLPGLPGEPGPEGIGVPGPKGDIGFRGLPGLPGPPGEGIQGPPGNIGRPGPPGPRGPQGDGIQGPKGEPGSQGMTGPRGPPGDGLPGAKGDRGLTGEKGGKGGKGDLGDSGVPGEAGRAGAKGDPGLTREDIIKLIKEICGCGIKCKERPMELVFVIDSSESVGPENFEIIKDFVIRLVDRTTVGRNATRIGLVLYSLDVHLEFNLARYATKQDIRHAIRKIPYMGEGTYTGTAIRKATQEAFLNARRGVSKVAIVITDGQTDKREPVKLDLAVREAHAANIEMYALGIVNASDPTQAEFLQELNLIASDPDDEHMYLIDDFNTLPALESKLVSQFCEDENGALIYNHITNGHWNGNSGHGDSVGDNNGQNSNSNRGFGKSGISSSDKEEIQNRRRHNSRSRGDTFTLPISADPLPVQVEEDDEGEDLDIRAQARISSIATLINKTSVVSVREGSISNDVVLSSSSSSSNTSTSSASTLSLNSNKLQSTVLPQEVSPLDPRCSLSLNQGPCRNYTIHWYYDKQANSCAQFWYGGCGGNDNRYETEEECKKTCVVSLRREG

Target ORF information:

RefSeq Version	XM_011609969.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609969.1

ORF Insert Sequence:

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAG 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGCTCAGCTA 
CATGAGGAGT GTGGTCTTGA GATCTCCTTC CTGTTGGACA GCTCTGAGAG CGCAAAGGAC 
AACCATGAGC AGGAGAAACA GTTTGTCATG AAGGTGGTGG ACAGACTCCA GGGGAAAAAG 
CTGCACACAG GCCGCAGCCT GAACCTGAGG GTGGCTCTGT TGCAGTACAG CAGTCACGTC 
ATCACGGAGC AAACCTTTAA GGACTGGAGG GGCACGGAGA ACTTCAAGGC TCGTGTAACT 
CCCATCATCT ACATCGGGCA CGGCACCTAC ACCACCTACG CCATCACCAA CATGACCAAG 
ATCTACCGGG AGGAGTCCAG TCCGGGCAGC ATCAGAGTGG CCGTGCTGCT CACAGATGGC 
ATTTCCCACC CGAGGAATCC GGATATTTTC TCTGCTGTGG CGGAAGCGAA AAACCAGGGC 
ATCAAATTCT TCACCCTGGG CATCACCCGC GCAGCCAACG AGCCCACCAA CATAGCCCAG 
CTCCGTCTTG TCTCCAGCTC CCCGGCCTCC CGCTTCCTCC ATAACCTGCA GGATGAAGAC 
ATTGTGGAGA AAATAGTTGC GGAGATTACC AGTTTGGCTG ATCAAGGATG CCCTTTGGCT 
CAGAAATGTG CTTGTGAGAA AGGAGAACGG GGCCTAAGTG GGCCTGCGGG CAAAAAAGGT 
CGGCCTGGAG AGGACGGAGC TCCAGGTGTT AAAGGGGAAA AGGGAGAATA TGGAATAAGT 
GGTCTACCTG GACAAGAGGG TCCTGAGGGT AAACCAGGTT ACAAGGGAGA GAAGGGAGAG 
AGAGGTGAGT GTGGTACTCC AGGAGTAAAA GGAGACGCGG GTCCTGTTGG TTTGGTCGGA 
ACGAGGGGAC CTCGGGGTTT GCAGGGGTTA CCAGGACCAC CAGGGGACAT TGGACCTGAG 
GGAATTCAGG GAAAGCAGGG CGAACGTGGC CCAACTGGCC CTCCAGGAAT TCAGGGGGAA 
ACTGGAATTG GACGTCCTGG TCCAAAGGGT GACATTGGAT TCCAAGGTCA GCCCGGTCCC 
CCCGGTCCTC GTGGAATAGG TGAACTAGGT CCTCCTGGTC CTCAAGGGCC GCAAGGTGTC 
CAAGGCAGTA AAGGACCCAC TGGTGAAGGT CTGCCTGGAC CAAAGGGGGA TCGGGGGCTC 
CCTGGACCGA GGGGGCCGAG GGGACAACAA GGAGTGGGAA TTAAAGGAGA TAAGGGGGAC 
TTTGGGCCTC CTGGTTTTCC AGGGCCAACT GGGCCAACAG GAGTTGGGAT ACAAGGAGAA 
AAGGGAGTTG AGGGTCCAAG AGGGCCACCA GGAGTCAGAG GACTTCCAGG CGAAGGTCTT 
CCTGGGCCTA AGGGAGATCA AGGTTTACCA GGAGAGCAAG GAGCTCCGGG AGATAGAGGT 
ATTGGTGAAG CCGGTCAAAA GGGAGAACCT GGGTCAGCTG GAATCGGAGG CCTCCCTGGT 
CTTCCAGGAG AGGATGGAGC ACCAGGGCAG AAGGGGGAGC CTGGTTTGCC TGGCTTAAGG 
GGAACCGAGG GAGCTCAAGG AATTGGTACT CAAGGGGAAA AGGGTGACCA GGGTCAAAGG 
GGCATTCGTG GATTACATGG GCCTCCTGGA ATCCCAGGAC CCTCTGGTCC AAAGGGTGAA 
CGTGGGATAC CAGGCCAGCA GGGCGTGCAG GGACAGCCAG GAAGGTCCAT ACCTGGTCCA 
AAGGGTGATG TTGGGTCTTC TGGTCCCCCT GGTCCAATTG GGGAGACAGG CCATGGATTA 
CCTGGTCCAA AGGGTGATCA TGGTCATCCA GGTTTACCAG GACCATATGG TCCCAAAGGT 
GAAGGTCTTC CTGGCCCTAT GGGTCCTACG GGTTTGCCAG GTTTACCAGG TGAGCCCGGT 
CCTGAAGGAA TTGGAGTTCC TGGTCCGAAG GGTGATATTG GCTTCAGAGG ATTGCCAGGA 
TTGCCTGGAC CACCTGGGGA GGGCATTCAA GGACCACCTG GTAATATTGG AAGGCCAGGG 
CCTCCTGGTC CAAGGGGACC ACAAGGAGAT GGTATTCAAG GCCCAAAGGG TGAGCCAGGA 
TCTCAGGGTA TGACAGGTCC CAGAGGGCCG CCGGGAGATG GACTCCCTGG AGCTAAGGGT 
GATCGGGGAT TAACTGGTGA AAAGGGGGGA AAAGGTGGAA AAGGAGACCT GGGAGATTCT 
GGAGTGCCTG GTGAAGCAGG AAGGGCGGGA GCAAAAGGAG ATCCAGGCCT CACCAGAGAG 
GACATCATTA AGCTCATTAA AGAAATCTGT GGATGTGGCA TCAAATGCAA AGAAAGACCG 
ATGGAGCTGG TGTTCGTCAT TGACAGCTCA GAGAGTGTTG GTCCAGAGAA TTTTGAAATC 
ATCAAGGACT TTGTCATCAG GCTGGTGGAC CGTACCACAG TTGGACGTAA CGCCACCAGA 
ATTGGACTAG TCCTCTACAG TCTGGATGTC CATTTAGAGT TCAACTTGGC TCGTTACGCG 
ACCAAGCAGG ACATCAGGCA CGCCATCAGA AAAATCCCTT ATATGGGCGA GGGCACCTAC 
ACTGGCACGG CAATCAGGAA GGCAACTCAG GAGGCTTTCT TAAACGCTCG GCGTGGCGTC 
AGTAAAGTGG CTATTGTCAT CACAGATGGT CAGACAGACA AACGAGAGCC AGTCAAGCTG 
GACCTGGCTG TGAGAGAAGC CCACGCTGCA AACATTGAGA TGTATGCCCT GGGCATTGTC 
AACGCTTCGG ATCCCACACA GGCCGAGTTC CTGCAGGAAC TCAACCTCAT CGCCTCTGAC 
CCTGACGATG AACACATGTA CCTCATCGAT GACTTCAATA CTCTACCAGC ACTGGAATCT 
AAATTGGTCA GCCAGTTCTG TGAGGATGAA AATGGAGCCC TGATTTACAA CCACATAACA 
AATGGACACT GGAACGGCAA CAGTGGACAT GGGGACAGTG TTGGTGACAA TAATGGCCAG 
AACAGTAACA GCAACAGAGG ATTTGGAAAG AGCGGGATCA GTTCCAGCGA TAAAGAGGAG 
ATCCAAAATC GGAGACGCCA CAACAGTCGA AGCCGTGGAG ATACTTTCAC ACTGCCCATA 
AGTGCCGATC CTCTTCCTGT GCAGGTGGAG GAGGATGATG AAGGTGAAGA TTTAGACATT 
AGAGCCCAAG CCCGCATCAG TAGTATTGCA ACATTAATTA ACAAAACATC TGTAGTATCT 
GTCAGAGAAG GCTCCATCTC TAATGATGTG GTTTTATCAT CTTCATCGTC TTCATCTAAC 
ACTTCAACCT CATCTGCATC AACTTTATCT TTAAACTCTA ACAAGTTACA GTCAACAGTC 
CTACCACAAG AGGTTTCTCC CCTTGATCCA CGCTGTAGTC TGAGTTTGAA TCAAGGTCCC 
TGTCGAAACT ACACCATCCA CTGGTACTAT GACAAGCAGG CCAACTCCTG CGCACAGTTT 
TGGTACGGAG GCTGTGGAGG AAATGATAAT CGTTATGAAA CGGAGGAAGA ATGCAAGAAA 
ACCTGTGTAG TAAGCCTTCG CAGAGAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101068515 ( XM_011609975.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011608277.1 Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X4, mRNA.

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CloneID	OTo24126
Clone ID Related Accession (Same CDS sequence)	XM_011609975.1
Accession Version	XM_011609975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-1(XXVIII) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAG 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTTGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGGCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATCA TGGTCATCCA GGTTTACCAG GACCATATGG TCCCAAAGGT 
GAAGGTCTTC CTGGCCCTAT GGGTCCTACG GGTTTGCCAG GTTTACCAGG TGAGCCCGGT 
CCTGAAGGAA TTGGAGTTCC TGGTCCGAAG GGTGATATTG GCTTCAGAGG ATTGCCAGGA 
TTGCCTGGAC CACCTGGGGA GGGCATTCAA GGACCACCTG GTAATATTGG AAGGCCAGGG 
CCTCCTGGTC CAAGGGGACC ACAAGGAGAT GGTATTCAAG GCCCAAAGGG TGAGCCAGGA 
TCTCAGGGTA TGACAGGTCC CAGAGGGCCG CCGGGAGATG GACTCCCTGG AGCTAAGGGT 
GATCGGGGAT TAACTGGTGA AAAGGGGGGA AAAGGTGGAA AAGGAGACCT GGGAGATTCT 
GGAGTGCCTG GTGAAGCAGG AAGGGCGGGA GCAAAAGGAG ATCCAGGCCT CACCAGAGAG 
GACATCATTA AGCTCATTAA AGAAATCTGT GGATGTGGCA TCAAATGCAA AGAAAGACCG 
ATGGAGCTGG TGTTCGTCAT TGACAGCTCA GAGAGTGTTG GTCCAGAGAA TTTTGAAATC 
ATCAAGGACT TTGTCATCAG GCTGGTGGAC CGTACCACAG TTGGACGTAA CGCCACCAGA 
ATTGGACTAG TCCTCTACAG TCTGGATGTC CATTTAGAGT TCAACTTGGC TCGTTACGCG 
ACCAAGCAGG ACATCAGGCA CGCCATCAGA AAAATCCCTT ATATGGGCGA GGGCACCTAC 
ACTGGCACGG CAATCAGGAA GGCAACTCAG GAGGCTTTCT TAAACGCTCG GCGTGGCGTC 
AGTAAAGTGG CTATTGTCAT CACAGATGGT CAGACAGACA AACGAGAGCC AGTCAAGCTG 
GACCTGGCTG TGAGAGAAGC CCACGCTGCA AACATTGAGA TGTATGCCCT GGGCATTGTC 
AACGCTTCGG ATCCCACACA GGCCGAGTTC CTGCAGGAAC TCAACCTCAT CGCCTCTGAC 
CCTGACGATG AACACATGTA CCTCATCGAT GACTTCAATA CTCTACCAGC ACTGGAATCT 
AAATTGGTCA GCCAGTTCTG TGAGGATGAA AATGGAGCCC TGATTTACAA CCACATAACA 
AATGGACACT GGAACGGCAA CAGTGGACAT GGGGACAGTG TTGGTGACAA TAATGGCCAG 
AACAGTAACA GCAACAGAGG ATTTGGAAAG AGCGGGATCA GTTCCAGCGA TAAAGAGGAG 
ATCCAAAATC GGAGACGCCA CAACAGTCGA AGCCGTGGAG ATACTTTCAC ACTGCCCATA 
AGTGCCGATC CTCTTCCTGT GCAGGTGGAG GAGGATGATG AAGGTGAAGA TTTAGACATT 
AGAGCCCAAG CCCGCATCAG TAGTATTGCA ACATTAATTA ACAAAACATC TGTAGTATCT 
GTCAGAGAAG GCTCCATCTC TAATGATGTG GTTTTATCAT CTTCATCGTC TTCATCTAAC 
ACTTCAACCT CATCTGCATC AACTTTATCT TTAAACTCTA ACAAGTTACA GTCAACAGTC 
CTACCACAAG AGGTTTCTCC CCTTGATCCA CGCTGTAGTC TGAGTTTGAA TCAAGGTCCC 
TGTCGAAACT ACACCATCCA CTGGTACTAT GACAAGCAGG CCAACTCCTG CGCACAGTTT 
TGGTACGGAG GCTGTGGAGG AAATGATAAT CGTTATGAAA CGGAGGAAGA ATGCAAGAAA 
ACCTGTGTAG TAAGCCTTCG CAGAGAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011608277.1
CDS	173..3745
Translation	MLSCTWVLLVTGLSCVWTQQTLEEKKPVNKVLSAGTHGRQAQLHEECGLEISFLLDSSESAKDNHEQEKQFVMKVVDRLQGKKLHTGRSLNLRVALLQYSSHVITEQTFKDWRGTENFKARVTPIIYIGHGTYTTYAITNMTKIYREESSPGSIRVAVLLTDGISHPRNPDIFSAVAEAKNQGIKFFTLGITRAANEPTNIAQLRLVSSSPASRFLHNLQDEDIVEKIVAEITSLADQGCPLAQKCACEKGERGLSGPAGKKGRPGEDGAPGVKGEKGEYGISGLPGQEGPEGKPGYKGEKGERGECGTPGVKGDAGPVGLVGTRGPRGLQGLPGPPGDIGPEGIQGKQGERGPTGPPGIQGETGIGRPGPKGDIGFQGQPGPPGPRGIGELGPPGPQGPQGVQGSKGPTGEGLPGPKGDRGLPGPRGPRGQQGVGIKGDKGDFGPPGFPGPTGPTGVGIQGEKGVEGPRGPPGVRGLPGEGLPGPKGDQGLPGEQGAPGDRGIGEAGQKGEPGSAGIGGLPGLPGEDGAPGQKGEPGLPGLRGTEGAQGIGTQGEKGDQGQRGIRGLHGPPGIPGPSGPKGERGIPGQQGVQGQPGRSIPGPKGDHGHPGLPGPYGPKGEGLPGPMGPTGLPGLPGEPGPEGIGVPGPKGDIGFRGLPGLPGPPGEGIQGPPGNIGRPGPPGPRGPQGDGIQGPKGEPGSQGMTGPRGPPGDGLPGAKGDRGLTGEKGGKGGKGDLGDSGVPGEAGRAGAKGDPGLTREDIIKLIKEICGCGIKCKERPMELVFVIDSSESVGPENFEIIKDFVIRLVDRTTVGRNATRIGLVLYSLDVHLEFNLARYATKQDIRHAIRKIPYMGEGTYTGTAIRKATQEAFLNARRGVSKVAIVITDGQTDKREPVKLDLAVREAHAANIEMYALGIVNASDPTQAEFLQELNLIASDPDDEHMYLIDDFNTLPALESKLVSQFCEDENGALIYNHITNGHWNGNSGHGDSVGDNNGQNSNSNRGFGKSGISSSDKEEIQNRRRHNSRSRGDTFTLPISADPLPVQVEEDDEGEDLDIRAQARISSIATLINKTSVVSVREGSISNDVVLSSSSSSSNTSTSSASTLSLNSNKLQSTVLPQEVSPLDPRCSLSLNQGPCRNYTIHWYYDKQANSCAQFWYGGCGGNDNRYETEEECKKTCVVSLRREG

Target ORF information:

RefSeq Version	XM_011609975.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609975.1

ORF Insert Sequence:

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAG 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTTGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGGCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATCA TGGTCATCCA GGTTTACCAG GACCATATGG TCCCAAAGGT 
GAAGGTCTTC CTGGCCCTAT GGGTCCTACG GGTTTGCCAG GTTTACCAGG TGAGCCCGGT 
CCTGAAGGAA TTGGAGTTCC TGGTCCGAAG GGTGATATTG GCTTCAGAGG ATTGCCAGGA 
TTGCCTGGAC CACCTGGGGA GGGCATTCAA GGACCACCTG GTAATATTGG AAGGCCAGGG 
CCTCCTGGTC CAAGGGGACC ACAAGGAGAT GGTATTCAAG GCCCAAAGGG TGAGCCAGGA 
TCTCAGGGTA TGACAGGTCC CAGAGGGCCG CCGGGAGATG GACTCCCTGG AGCTAAGGGT 
GATCGGGGAT TAACTGGTGA AAAGGGGGGA AAAGGTGGAA AAGGAGACCT GGGAGATTCT 
GGAGTGCCTG GTGAAGCAGG AAGGGCGGGA GCAAAAGGAG ATCCAGGCCT CACCAGAGAG 
GACATCATTA AGCTCATTAA AGAAATCTGT GGATGTGGCA TCAAATGCAA AGAAAGACCG 
ATGGAGCTGG TGTTCGTCAT TGACAGCTCA GAGAGTGTTG GTCCAGAGAA TTTTGAAATC 
ATCAAGGACT TTGTCATCAG GCTGGTGGAC CGTACCACAG TTGGACGTAA CGCCACCAGA 
ATTGGACTAG TCCTCTACAG TCTGGATGTC CATTTAGAGT TCAACTTGGC TCGTTACGCG 
ACCAAGCAGG ACATCAGGCA CGCCATCAGA AAAATCCCTT ATATGGGCGA GGGCACCTAC 
ACTGGCACGG CAATCAGGAA GGCAACTCAG GAGGCTTTCT TAAACGCTCG GCGTGGCGTC 
AGTAAAGTGG CTATTGTCAT CACAGATGGT CAGACAGACA AACGAGAGCC AGTCAAGCTG 
GACCTGGCTG TGAGAGAAGC CCACGCTGCA AACATTGAGA TGTATGCCCT GGGCATTGTC 
AACGCTTCGG ATCCCACACA GGCCGAGTTC CTGCAGGAAC TCAACCTCAT CGCCTCTGAC 
CCTGACGATG AACACATGTA CCTCATCGAT GACTTCAATA CTCTACCAGC ACTGGAATCT 
AAATTGGTCA GCCAGTTCTG TGAGGATGAA AATGGAGCCC TGATTTACAA CCACATAACA 
AATGGACACT GGAACGGCAA CAGTGGACAT GGGGACAGTG TTGGTGACAA TAATGGCCAG 
AACAGTAACA GCAACAGAGG ATTTGGAAAG AGCGGGATCA GTTCCAGCGA TAAAGAGGAG 
ATCCAAAATC GGAGACGCCA CAACAGTCGA AGCCGTGGAG ATACTTTCAC ACTGCCCATA 
AGTGCCGATC CTCTTCCTGT GCAGGTGGAG GAGGATGATG AAGGTGAAGA TTTAGACATT 
AGAGCCCAAG CCCGCATCAG TAGTATTGCA ACATTAATTA ACAAAACATC TGTAGTATCT 
GTCAGAGAAG GCTCCATCTC TAATGATGTG GTTTTATCAT CTTCATCGTC TTCATCTAAC 
ACTTCAACCT CATCTGCATC AACTTTATCT TTAAACTCTA ACAAGTTACA GTCAACAGTC 
CTACCACAAG AGGTTTCTCC CCTTGATCCA CGCTGTAGTC TGAGTTTGAA TCAAGGTCCC 
TGTCGAAACT ACACCATCCA CTGGTACTAT GACAAGCAGG CCAACTCCTG CGCACAGTTT 
TGGTACGGAG GCTGTGGAGG AAATGATAAT CGTTATGAAA CGGAGGAAGA ATGCAAGAAA 
ACCTGTGTAG TAAGCCTTCG CAGAGAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101068515 ( XM_011609955.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011608257.2 Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X1, mRNA.

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CloneID	OTo43047
Clone ID Related Accession (Same CDS sequence)	XM_011609955.2 , XM_011609959.2
Accession Version	XM_011609955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XXVIII) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011609955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAC 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTCGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGTCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATGT TGGGTCTTCT GGTCCCCCTG GTCCAATTGG GGAGACAGGC 
CATGGATTAC CTGGTCCAAA GGGTGATCAT GGTCATCCAG GTTTACCAGG ACCATATGGT 
CCCAAAGGTG AAGGTCTTCC TGGCCCTATG GGTCCTACGG GTTTGCCAGG TTTACCAGGT 
GAGCCCGGTC CTGAAGGAAT TGGAGTTCCT GGTCCGAAGG GTGATATTGG CTTCAGAGGA 
TTGCCAGGAT TGCCTGGACC ACCTGGGGAG GGCATTCAAG GACCACCTGG TAATATTGGA 
AGGCCAGGGC CTCCTGGTCC AAGGGGACCA CAAGGAGATG GTATTCAAGG CCCAAAGGGT 
GAGCCAGGAT CTCAGGGTAT GACAGGTCCC AGAGGGCCGC CGGGAGATGG ACTCCCTGGA 
GCTAAGGGTG ATCGGGGATT AACTGGTGAA AAGGGGGGAA AAGGTGGAAA AGGAGACCTG 
GGAGATTCTG GAGTGCCTGG TGAAGCAGGA AGGGCGGGAG CAAAAGGAGA TCCAGGCCTC 
ACCAGAGAGG ACATCATTAA GCTCATTAAA GAAATCTGTG GATGTGGCAT CAAATGCAAA 
GAAAGACCGA TGGAGCTGGT GTTCGTCATT GACAGCTCAG AGAGTGTTGG TCCAGAGAAT 
TTTGAAATCA TCAAGGACTT TGTCATCAGG CTGGTGGACC GTACCACAGT TGGACGTAAC 
GCCACCAGAA TTGGACTAGT CCTCTACAGT CTGGATGTCC ATTTAGAGTT CAACTTGGCT 
CGTTACGCGA CCAAGCAGGA CATCAGGCAC GCCATCAGAA AAATCCCTTA TATGGGCGAG 
GGCACCTACA CTGGCACGGC AATCAGGAAG GCAACTCAGG AGGCTTTCTT AAACGCTCGG 
CGTGGCGTCA GTAAAGTGGC TATTGTCATC ACAGATGGTC AGACAGACAA ACGAGAGCCA 
GTCAAGCTGG ACCTGGCTGT GAGAGAAGCC CACGCTGCAA ACATTGAGAT GTATGCCCTG 
GGCATTGTCA ACGCTTCGGA TCCCACACAG GCCGAGTTCC TGCAAGAACT CAACCTCATC 
GCCTCTGACC CTGACGATGA ACACATGTAC CTCATCGATG ACTTCAATAC TCTACCAGCA 
CTGGAATCTA AATTGGTCAG CCAGTTCTGT GAGGATGAAA ATGGAGCCCT GATTTACAAC 
CACATAACAA ATGGACACTG GAACGGCAAC AGTGGACATG GGGACAGTGT TGGTGACAAT 
AATGGCCAGA ACAGTAACAG CAACAGAGGA TTTGGAAAGA GCGGGATCAG TTCCAGCGAT 
AAAGAGGAGA TCCAAAATCG GAGACGCCAC AACAGTCGAA GCCGTGGAGA TACTTTCACA 
CTGCCCATAA GTGCCGATCC TCTTCCTGTG CAGGTGGAGG AGGATGATGA AGGTGAAGAT 
TTAGACATTA GAGCCCAAGC CCGCATCAGT AGTATTGCAA CATTAATTAA CAAAACATCT 
GTAGTATCTG TCAGAGAAGG CTCCATCTCT AATGATGTGG TTTTATCATC TTCATCGTCT 
TCATCTAACA CTTCAACCTC ATCTGCATCA ACTTTATCTT TAAACTCTAA CAAGTTACAG 
TCAACAGTCC TACCACAAGA GGTTTCTCCC CTTGATCCAC GCTGTAGTCT GAGTTTGAAT 
CAAGGTCCCT GTCGAAACTA CACCATCCAC TGGTACTATG ACAAGCAGGC CAACTCCTGC 
GCACAGTTTT GGTACGGAGG CTGTGGAGGA AATGATAATC GTTATGAAAC AGAGGAAGAA 
TGCAAGAAAA CCTGTGTAGT AAGCCTTCGC AGAGGAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011608257.2
CDS	174..3815
Translation	MLSCTWVLLVTGLSCVWTQHTLEEKKPVNKVLSAGTHGRQAQLHEECGLEISFLLDSSESAKDNHEQEKQFVMKVVDRLQGKKLHTGRSLNLRVALLQYSSHVITEQTFKDWRGTENFKARVTPIIYIGHGTYTTYAITNMTKIYREESSPGSIRVAVLLTDGISHPRNPDIFSAVAEAKNQGIKFFTLGITRAANEPTNIAQLRLVSSSPASRFLHNLQDEDIVEKIVAEITSLADQGCPLAQKCACEKGERGLSGPAGKKGRPGEDGAPGVKGEKGEYGISGLPGQEGPEGKPGYKGEKGERGECGTPGVKGDAGPVGLVGTRGPRGLQGLPGPPGDIGPEGIQGKQGERGPTGPPGIQGETGIGRPGPKGDIGFQGQPGPPGPRGIGELGPPGPQGPQGVQGSKGPTGEGLPGPKGDRGLPGPRGPRGQQGVGIKGDKGDFGPPGFPGPTGPTGVGIQGEKGVEGPRGPPGVRGLPGEGLPGPKGDQGLPGEQGAPGDRGIGEAGQKGEPGSAGIGGLPGLPGEDGAPGQKGEPGLPGLRGTEGAQGIGTQGEKGDQGQRGIRGLHGPPGIPGPSGPKGERGIPGQQGVQGQPGRSIPGPKGDVGSSGPPGPIGETGHGLPGPKGDHGHPGLPGPYGPKGEGLPGPMGPTGLPGLPGEPGPEGIGVPGPKGDIGFRGLPGLPGPPGEGIQGPPGNIGRPGPPGPRGPQGDGIQGPKGEPGSQGMTGPRGPPGDGLPGAKGDRGLTGEKGGKGGKGDLGDSGVPGEAGRAGAKGDPGLTREDIIKLIKEICGCGIKCKERPMELVFVIDSSESVGPENFEIIKDFVIRLVDRTTVGRNATRIGLVLYSLDVHLEFNLARYATKQDIRHAIRKIPYMGEGTYTGTAIRKATQEAFLNARRGVSKVAIVITDGQTDKREPVKLDLAVREAHAANIEMYALGIVNASDPTQAEFLQELNLIASDPDDEHMYLIDDFNTLPALESKLVSQFCEDENGALIYNHITNGHWNGNSGHGDSVGDNNGQNSNSNRGFGKSGISSSDKEEIQNRRRHNSRSRGDTFTLPISADPLPVQVEEDDEGEDLDIRAQARISSIATLINKTSVVSVREGSISNDVVLSSSSSSSNTSTSSASTLSLNSNKLQSTVLPQEVSPLDPRCSLSLNQGPCRNYTIHWYYDKQANSCAQFWYGGCGGNDNRYETEEECKKTCVVSLRRGG

Target ORF information:

RefSeq Version	XM_011609955.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609955.2

ORF Insert Sequence:

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAC 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTCGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGTCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATGT TGGGTCTTCT GGTCCCCCTG GTCCAATTGG GGAGACAGGC 
CATGGATTAC CTGGTCCAAA GGGTGATCAT GGTCATCCAG GTTTACCAGG ACCATATGGT 
CCCAAAGGTG AAGGTCTTCC TGGCCCTATG GGTCCTACGG GTTTGCCAGG TTTACCAGGT 
GAGCCCGGTC CTGAAGGAAT TGGAGTTCCT GGTCCGAAGG GTGATATTGG CTTCAGAGGA 
TTGCCAGGAT TGCCTGGACC ACCTGGGGAG GGCATTCAAG GACCACCTGG TAATATTGGA 
AGGCCAGGGC CTCCTGGTCC AAGGGGACCA CAAGGAGATG GTATTCAAGG CCCAAAGGGT 
GAGCCAGGAT CTCAGGGTAT GACAGGTCCC AGAGGGCCGC CGGGAGATGG ACTCCCTGGA 
GCTAAGGGTG ATCGGGGATT AACTGGTGAA AAGGGGGGAA AAGGTGGAAA AGGAGACCTG 
GGAGATTCTG GAGTGCCTGG TGAAGCAGGA AGGGCGGGAG CAAAAGGAGA TCCAGGCCTC 
ACCAGAGAGG ACATCATTAA GCTCATTAAA GAAATCTGTG GATGTGGCAT CAAATGCAAA 
GAAAGACCGA TGGAGCTGGT GTTCGTCATT GACAGCTCAG AGAGTGTTGG TCCAGAGAAT 
TTTGAAATCA TCAAGGACTT TGTCATCAGG CTGGTGGACC GTACCACAGT TGGACGTAAC 
GCCACCAGAA TTGGACTAGT CCTCTACAGT CTGGATGTCC ATTTAGAGTT CAACTTGGCT 
CGTTACGCGA CCAAGCAGGA CATCAGGCAC GCCATCAGAA AAATCCCTTA TATGGGCGAG 
GGCACCTACA CTGGCACGGC AATCAGGAAG GCAACTCAGG AGGCTTTCTT AAACGCTCGG 
CGTGGCGTCA GTAAAGTGGC TATTGTCATC ACAGATGGTC AGACAGACAA ACGAGAGCCA 
GTCAAGCTGG ACCTGGCTGT GAGAGAAGCC CACGCTGCAA ACATTGAGAT GTATGCCCTG 
GGCATTGTCA ACGCTTCGGA TCCCACACAG GCCGAGTTCC TGCAAGAACT CAACCTCATC 
GCCTCTGACC CTGACGATGA ACACATGTAC CTCATCGATG ACTTCAATAC TCTACCAGCA 
CTGGAATCTA AATTGGTCAG CCAGTTCTGT GAGGATGAAA ATGGAGCCCT GATTTACAAC 
CACATAACAA ATGGACACTG GAACGGCAAC AGTGGACATG GGGACAGTGT TGGTGACAAT 
AATGGCCAGA ACAGTAACAG CAACAGAGGA TTTGGAAAGA GCGGGATCAG TTCCAGCGAT 
AAAGAGGAGA TCCAAAATCG GAGACGCCAC AACAGTCGAA GCCGTGGAGA TACTTTCACA 
CTGCCCATAA GTGCCGATCC TCTTCCTGTG CAGGTGGAGG AGGATGATGA AGGTGAAGAT 
TTAGACATTA GAGCCCAAGC CCGCATCAGT AGTATTGCAA CATTAATTAA CAAAACATCT 
GTAGTATCTG TCAGAGAAGG CTCCATCTCT AATGATGTGG TTTTATCATC TTCATCGTCT 
TCATCTAACA CTTCAACCTC ATCTGCATCA ACTTTATCTT TAAACTCTAA CAAGTTACAG 
TCAACAGTCC TACCACAAGA GGTTTCTCCC CTTGATCCAC GCTGTAGTCT GAGTTTGAAT 
CAAGGTCCCT GTCGAAACTA CACCATCCAC TGGTACTATG ACAAGCAGGC CAACTCCTGC 
GCACAGTTTT GGTACGGAGG CTGTGGAGGA AATGATAATC GTTATGAAAC AGAGGAAGAA 
TGCAAGAAAA CCTGTGTAGT AAGCCTTCGC AGAGGAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101068515 ( XM_011609959.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011608261.2 Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X2, mRNA.

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CloneID	OTo43047
Clone ID Related Accession (Same CDS sequence)	XM_011609955.2 , XM_011609959.2
Accession Version	XM_011609959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XXVIII) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011609959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAC 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTCGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGTCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATGT TGGGTCTTCT GGTCCCCCTG GTCCAATTGG GGAGACAGGC 
CATGGATTAC CTGGTCCAAA GGGTGATCAT GGTCATCCAG GTTTACCAGG ACCATATGGT 
CCCAAAGGTG AAGGTCTTCC TGGCCCTATG GGTCCTACGG GTTTGCCAGG TTTACCAGGT 
GAGCCCGGTC CTGAAGGAAT TGGAGTTCCT GGTCCGAAGG GTGATATTGG CTTCAGAGGA 
TTGCCAGGAT TGCCTGGACC ACCTGGGGAG GGCATTCAAG GACCACCTGG TAATATTGGA 
AGGCCAGGGC CTCCTGGTCC AAGGGGACCA CAAGGAGATG GTATTCAAGG CCCAAAGGGT 
GAGCCAGGAT CTCAGGGTAT GACAGGTCCC AGAGGGCCGC CGGGAGATGG ACTCCCTGGA 
GCTAAGGGTG ATCGGGGATT AACTGGTGAA AAGGGGGGAA AAGGTGGAAA AGGAGACCTG 
GGAGATTCTG GAGTGCCTGG TGAAGCAGGA AGGGCGGGAG CAAAAGGAGA TCCAGGCCTC 
ACCAGAGAGG ACATCATTAA GCTCATTAAA GAAATCTGTG GATGTGGCAT CAAATGCAAA 
GAAAGACCGA TGGAGCTGGT GTTCGTCATT GACAGCTCAG AGAGTGTTGG TCCAGAGAAT 
TTTGAAATCA TCAAGGACTT TGTCATCAGG CTGGTGGACC GTACCACAGT TGGACGTAAC 
GCCACCAGAA TTGGACTAGT CCTCTACAGT CTGGATGTCC ATTTAGAGTT CAACTTGGCT 
CGTTACGCGA CCAAGCAGGA CATCAGGCAC GCCATCAGAA AAATCCCTTA TATGGGCGAG 
GGCACCTACA CTGGCACGGC AATCAGGAAG GCAACTCAGG AGGCTTTCTT AAACGCTCGG 
CGTGGCGTCA GTAAAGTGGC TATTGTCATC ACAGATGGTC AGACAGACAA ACGAGAGCCA 
GTCAAGCTGG ACCTGGCTGT GAGAGAAGCC CACGCTGCAA ACATTGAGAT GTATGCCCTG 
GGCATTGTCA ACGCTTCGGA TCCCACACAG GCCGAGTTCC TGCAAGAACT CAACCTCATC 
GCCTCTGACC CTGACGATGA ACACATGTAC CTCATCGATG ACTTCAATAC TCTACCAGCA 
CTGGAATCTA AATTGGTCAG CCAGTTCTGT GAGGATGAAA ATGGAGCCCT GATTTACAAC 
CACATAACAA ATGGACACTG GAACGGCAAC AGTGGACATG GGGACAGTGT TGGTGACAAT 
AATGGCCAGA ACAGTAACAG CAACAGAGGA TTTGGAAAGA GCGGGATCAG TTCCAGCGAT 
AAAGAGGAGA TCCAAAATCG GAGACGCCAC AACAGTCGAA GCCGTGGAGA TACTTTCACA 
CTGCCCATAA GTGCCGATCC TCTTCCTGTG CAGGTGGAGG AGGATGATGA AGGTGAAGAT 
TTAGACATTA GAGCCCAAGC CCGCATCAGT AGTATTGCAA CATTAATTAA CAAAACATCT 
GTAGTATCTG TCAGAGAAGG CTCCATCTCT AATGATGTGG TTTTATCATC TTCATCGTCT 
TCATCTAACA CTTCAACCTC ATCTGCATCA ACTTTATCTT TAAACTCTAA CAAGTTACAG 
TCAACAGTCC TACCACAAGA GGTTTCTCCC CTTGATCCAC GCTGTAGTCT GAGTTTGAAT 
CAAGGTCCCT GTCGAAACTA CACCATCCAC TGGTACTATG ACAAGCAGGC CAACTCCTGC 
GCACAGTTTT GGTACGGAGG CTGTGGAGGA AATGATAATC GTTATGAAAC AGAGGAAGAA 
TGCAAGAAAA CCTGTGTAGT AAGCCTTCGC AGAGGAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011608261.2
CDS	182..3823
Translation	MLSCTWVLLVTGLSCVWTQHTLEEKKPVNKVLSAGTHGRQAQLHEECGLEISFLLDSSESAKDNHEQEKQFVMKVVDRLQGKKLHTGRSLNLRVALLQYSSHVITEQTFKDWRGTENFKARVTPIIYIGHGTYTTYAITNMTKIYREESSPGSIRVAVLLTDGISHPRNPDIFSAVAEAKNQGIKFFTLGITRAANEPTNIAQLRLVSSSPASRFLHNLQDEDIVEKIVAEITSLADQGCPLAQKCACEKGERGLSGPAGKKGRPGEDGAPGVKGEKGEYGISGLPGQEGPEGKPGYKGEKGERGECGTPGVKGDAGPVGLVGTRGPRGLQGLPGPPGDIGPEGIQGKQGERGPTGPPGIQGETGIGRPGPKGDIGFQGQPGPPGPRGIGELGPPGPQGPQGVQGSKGPTGEGLPGPKGDRGLPGPRGPRGQQGVGIKGDKGDFGPPGFPGPTGPTGVGIQGEKGVEGPRGPPGVRGLPGEGLPGPKGDQGLPGEQGAPGDRGIGEAGQKGEPGSAGIGGLPGLPGEDGAPGQKGEPGLPGLRGTEGAQGIGTQGEKGDQGQRGIRGLHGPPGIPGPSGPKGERGIPGQQGVQGQPGRSIPGPKGDVGSSGPPGPIGETGHGLPGPKGDHGHPGLPGPYGPKGEGLPGPMGPTGLPGLPGEPGPEGIGVPGPKGDIGFRGLPGLPGPPGEGIQGPPGNIGRPGPPGPRGPQGDGIQGPKGEPGSQGMTGPRGPPGDGLPGAKGDRGLTGEKGGKGGKGDLGDSGVPGEAGRAGAKGDPGLTREDIIKLIKEICGCGIKCKERPMELVFVIDSSESVGPENFEIIKDFVIRLVDRTTVGRNATRIGLVLYSLDVHLEFNLARYATKQDIRHAIRKIPYMGEGTYTGTAIRKATQEAFLNARRGVSKVAIVITDGQTDKREPVKLDLAVREAHAANIEMYALGIVNASDPTQAEFLQELNLIASDPDDEHMYLIDDFNTLPALESKLVSQFCEDENGALIYNHITNGHWNGNSGHGDSVGDNNGQNSNSNRGFGKSGISSSDKEEIQNRRRHNSRSRGDTFTLPISADPLPVQVEEDDEGEDLDIRAQARISSIATLINKTSVVSVREGSISNDVVLSSSSSSSNTSTSSASTLSLNSNKLQSTVLPQEVSPLDPRCSLSLNQGPCRNYTIHWYYDKQANSCAQFWYGGCGGNDNRYETEEECKKTCVVSLRRGG

Target ORF information:

RefSeq Version	XM_011609959.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609959.2

ORF Insert Sequence:

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAC 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTCGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGTCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATGT TGGGTCTTCT GGTCCCCCTG GTCCAATTGG GGAGACAGGC 
CATGGATTAC CTGGTCCAAA GGGTGATCAT GGTCATCCAG GTTTACCAGG ACCATATGGT 
CCCAAAGGTG AAGGTCTTCC TGGCCCTATG GGTCCTACGG GTTTGCCAGG TTTACCAGGT 
GAGCCCGGTC CTGAAGGAAT TGGAGTTCCT GGTCCGAAGG GTGATATTGG CTTCAGAGGA 
TTGCCAGGAT TGCCTGGACC ACCTGGGGAG GGCATTCAAG GACCACCTGG TAATATTGGA 
AGGCCAGGGC CTCCTGGTCC AAGGGGACCA CAAGGAGATG GTATTCAAGG CCCAAAGGGT 
GAGCCAGGAT CTCAGGGTAT GACAGGTCCC AGAGGGCCGC CGGGAGATGG ACTCCCTGGA 
GCTAAGGGTG ATCGGGGATT AACTGGTGAA AAGGGGGGAA AAGGTGGAAA AGGAGACCTG 
GGAGATTCTG GAGTGCCTGG TGAAGCAGGA AGGGCGGGAG CAAAAGGAGA TCCAGGCCTC 
ACCAGAGAGG ACATCATTAA GCTCATTAAA GAAATCTGTG GATGTGGCAT CAAATGCAAA 
GAAAGACCGA TGGAGCTGGT GTTCGTCATT GACAGCTCAG AGAGTGTTGG TCCAGAGAAT 
TTTGAAATCA TCAAGGACTT TGTCATCAGG CTGGTGGACC GTACCACAGT TGGACGTAAC 
GCCACCAGAA TTGGACTAGT CCTCTACAGT CTGGATGTCC ATTTAGAGTT CAACTTGGCT 
CGTTACGCGA CCAAGCAGGA CATCAGGCAC GCCATCAGAA AAATCCCTTA TATGGGCGAG 
GGCACCTACA CTGGCACGGC AATCAGGAAG GCAACTCAGG AGGCTTTCTT AAACGCTCGG 
CGTGGCGTCA GTAAAGTGGC TATTGTCATC ACAGATGGTC AGACAGACAA ACGAGAGCCA 
GTCAAGCTGG ACCTGGCTGT GAGAGAAGCC CACGCTGCAA ACATTGAGAT GTATGCCCTG 
GGCATTGTCA ACGCTTCGGA TCCCACACAG GCCGAGTTCC TGCAAGAACT CAACCTCATC 
GCCTCTGACC CTGACGATGA ACACATGTAC CTCATCGATG ACTTCAATAC TCTACCAGCA 
CTGGAATCTA AATTGGTCAG CCAGTTCTGT GAGGATGAAA ATGGAGCCCT GATTTACAAC 
CACATAACAA ATGGACACTG GAACGGCAAC AGTGGACATG GGGACAGTGT TGGTGACAAT 
AATGGCCAGA ACAGTAACAG CAACAGAGGA TTTGGAAAGA GCGGGATCAG TTCCAGCGAT 
AAAGAGGAGA TCCAAAATCG GAGACGCCAC AACAGTCGAA GCCGTGGAGA TACTTTCACA 
CTGCCCATAA GTGCCGATCC TCTTCCTGTG CAGGTGGAGG AGGATGATGA AGGTGAAGAT 
TTAGACATTA GAGCCCAAGC CCGCATCAGT AGTATTGCAA CATTAATTAA CAAAACATCT 
GTAGTATCTG TCAGAGAAGG CTCCATCTCT AATGATGTGG TTTTATCATC TTCATCGTCT 
TCATCTAACA CTTCAACCTC ATCTGCATCA ACTTTATCTT TAAACTCTAA CAAGTTACAG 
TCAACAGTCC TACCACAAGA GGTTTCTCCC CTTGATCCAC GCTGTAGTCT GAGTTTGAAT 
CAAGGTCCCT GTCGAAACTA CACCATCCAC TGGTACTATG ACAAGCAGGC CAACTCCTGC 
GCACAGTTTT GGTACGGAGG CTGTGGAGGA AATGATAATC GTTATGAAAC AGAGGAAGAA 
TGCAAGAAAA CCTGTGTAGT AAGCCTTCGC AGAGGAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101068515 ( XM_011609969.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011608271.2 Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo43048
Clone ID Related Accession (Same CDS sequence)	XM_011609969.2
Accession Version	XM_011609969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XXVIII) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011609969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAC 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGCTCAGCTA 
CATGAGGAGT GTGGTCTTGA GATCTCCTTC CTGTTGGACA GCTCTGAGAG CGCAAAGGAC 
AACCATGAGC AGGAGAAACA GTTTGTCATG AAGGTGGTGG ACAGACTCCA GGGGAAAAAG 
CTGCACACAG GCCGCAGCCT GAACCTGAGG GTGGCTCTGT TGCAGTACAG CAGTCACGTC 
ATCACGGAGC AAACCTTTAA GGACTGGAGG GGCACGGAGA ACTTCAAGGC TCGTGTAACT 
CCCATCATCT ACATCGGGCA CGGCACCTAC ACCACCTACG CCATCACCAA CATGACCAAG 
ATCTACCGGG AGGAGTCCAG TCCGGGCAGC ATCAGAGTGG CCGTGCTGCT CACAGATGGC 
ATTTCCCACC CGAGGAATCC GGATATTTTC TCTGCTGTGG CGGAAGCGAA AAACCAGGGC 
ATCAAATTCT TCACCCTGGG CATCACCCGC GCAGCCAACG AGCCCACCAA CATAGCCCAG 
CTCCGTCTTG TCTCCAGCTC CCCGGCCTCC CGCTTCCTCC ATAACCTGCA GGATGAAGAC 
ATTGTGGAGA AAATAGTCGC GGAGATTACC AGTTTGGCTG ATCAAGGATG CCCTTTGGCT 
CAGAAATGTG CTTGTGAGAA AGGAGAACGG GGCCTAAGTG GGCCTGCGGG CAAAAAAGGT 
CGGCCTGGAG AGGACGGAGC TCCAGGTGTT AAAGGGGAAA AGGGAGAATA TGGAATAAGT 
GGTCTACCTG GACAAGAGGG TCCTGAGGGT AAACCAGGTT ACAAGGGAGA GAAGGGAGAG 
AGAGGTGAGT GTGGTACTCC AGGAGTAAAA GGAGACGCGG GTCCTGTTGG TTTGGTCGGA 
ACGAGGGGAC CTCGGGGTTT GCAGGGGTTA CCAGGACCAC CAGGGGACAT TGGACCTGAG 
GGAATTCAGG GAAAGCAGGG CGAACGTGGC CCAACTGGCC CTCCAGGAAT TCAGGGGGAA 
ACTGGAATTG GACGTCCTGG TCCAAAGGGT GACATTGGAT TCCAAGGTCA GCCCGGTCCC 
CCCGGTCCTC GTGGAATAGG TGAACTAGGT CCTCCTGGTC CTCAAGGGCC GCAAGGTGTC 
CAAGGCAGTA AAGGACCCAC TGGTGAAGGT CTGCCTGGAC CAAAGGGGGA TCGGGGGCTC 
CCTGGACCGA GGGGGCCGAG GGGACAACAA GGAGTGGGAA TTAAAGGAGA TAAGGGGGAC 
TTTGGGCCTC CTGGTTTTCC AGGGCCAACT GGGCCAACAG GAGTTGGGAT ACAAGGAGAA 
AAGGGAGTTG AGGGTCCAAG AGGGCCACCA GGAGTCAGAG GACTTCCAGG CGAAGGTCTT 
CCTGGTCCTA AGGGAGATCA AGGTTTACCA GGAGAGCAAG GAGCTCCGGG AGATAGAGGT 
ATTGGTGAAG CCGGTCAAAA GGGAGAACCT GGGTCAGCTG GAATCGGAGG CCTCCCTGGT 
CTTCCAGGAG AGGATGGAGC ACCAGGGCAG AAGGGGGAGC CTGGTTTGCC TGGCTTAAGG 
GGAACCGAGG GAGCTCAAGG AATTGGTACT CAAGGGGAAA AGGGTGACCA GGGTCAAAGG 
GGCATTCGTG GATTACATGG GCCTCCTGGA ATCCCAGGAC CCTCTGGTCC AAAGGGTGAA 
CGTGGGATAC CAGGCCAGCA GGGCGTGCAG GGACAGCCAG GAAGGTCCAT ACCTGGTCCA 
AAGGGTGATG TTGGGTCTTC TGGTCCCCCT GGTCCAATTG GGGAGACAGG CCATGGATTA 
CCTGGTCCAA AGGGTGATCA TGGTCATCCA GGTTTACCAG GACCATATGG TCCCAAAGGT 
GAAGGTCTTC CTGGCCCTAT GGGTCCTACG GGTTTGCCAG GTTTACCAGG TGAGCCCGGT 
CCTGAAGGAA TTGGAGTTCC TGGTCCGAAG GGTGATATTG GCTTCAGAGG ATTGCCAGGA 
TTGCCTGGAC CACCTGGGGA GGGCATTCAA GGACCACCTG GTAATATTGG AAGGCCAGGG 
CCTCCTGGTC CAAGGGGACC ACAAGGAGAT GGTATTCAAG GCCCAAAGGG TGAGCCAGGA 
TCTCAGGGTA TGACAGGTCC CAGAGGGCCG CCGGGAGATG GACTCCCTGG AGCTAAGGGT 
GATCGGGGAT TAACTGGTGA AAAGGGGGGA AAAGGTGGAA AAGGAGACCT GGGAGATTCT 
GGAGTGCCTG GTGAAGCAGG AAGGGCGGGA GCAAAAGGAG ATCCAGGCCT CACCAGAGAG 
GACATCATTA AGCTCATTAA AGAAATCTGT GGATGTGGCA TCAAATGCAA AGAAAGACCG 
ATGGAGCTGG TGTTCGTCAT TGACAGCTCA GAGAGTGTTG GTCCAGAGAA TTTTGAAATC 
ATCAAGGACT TTGTCATCAG GCTGGTGGAC CGTACCACAG TTGGACGTAA CGCCACCAGA 
ATTGGACTAG TCCTCTACAG TCTGGATGTC CATTTAGAGT TCAACTTGGC TCGTTACGCG 
ACCAAGCAGG ACATCAGGCA CGCCATCAGA AAAATCCCTT ATATGGGCGA GGGCACCTAC 
ACTGGCACGG CAATCAGGAA GGCAACTCAG GAGGCTTTCT TAAACGCTCG GCGTGGCGTC 
AGTAAAGTGG CTATTGTCAT CACAGATGGT CAGACAGACA AACGAGAGCC AGTCAAGCTG 
GACCTGGCTG TGAGAGAAGC CCACGCTGCA AACATTGAGA TGTATGCCCT GGGCATTGTC 
AACGCTTCGG ATCCCACACA GGCCGAGTTC CTGCAAGAAC TCAACCTCAT CGCCTCTGAC 
CCTGACGATG AACACATGTA CCTCATCGAT GACTTCAATA CTCTACCAGC ACTGGAATCT 
AAATTGGTCA GCCAGTTCTG TGAGGATGAA AATGGAGCCC TGATTTACAA CCACATAACA 
AATGGACACT GGAACGGCAA CAGTGGACAT GGGGACAGTG TTGGTGACAA TAATGGCCAG 
AACAGTAACA GCAACAGAGG ATTTGGAAAG AGCGGGATCA GTTCCAGCGA TAAAGAGGAG 
ATCCAAAATC GGAGACGCCA CAACAGTCGA AGCCGTGGAG ATACTTTCAC ACTGCCCATA 
AGTGCCGATC CTCTTCCTGT GCAGGTGGAG GAGGATGATG AAGGTGAAGA TTTAGACATT 
AGAGCCCAAG CCCGCATCAG TAGTATTGCA ACATTAATTA ACAAAACATC TGTAGTATCT 
GTCAGAGAAG GCTCCATCTC TAATGATGTG GTTTTATCAT CTTCATCGTC TTCATCTAAC 
ACTTCAACCT CATCTGCATC AACTTTATCT TTAAACTCTA ACAAGTTACA GTCAACAGTC 
CTACCACAAG AGGTTTCTCC CCTTGATCCA CGCTGTAGTC TGAGTTTGAA TCAAGGTCCC 
TGTCGAAACT ACACCATCCA CTGGTACTAT GACAAGCAGG CCAACTCCTG CGCACAGTTT 
TGGTACGGAG GCTGTGGAGG AAATGATAAT CGTTATGAAA CAGAGGAAGA ATGCAAGAAA 
ACCTGTGTAG TAAGCCTTCG CAGAGGAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011608271.2
CDS	174..3806
Translation	MLSCTWVLLVTGLSCVWTQHTLEEKKPVNKVLSAGTHAQLHEECGLEISFLLDSSESAKDNHEQEKQFVMKVVDRLQGKKLHTGRSLNLRVALLQYSSHVITEQTFKDWRGTENFKARVTPIIYIGHGTYTTYAITNMTKIYREESSPGSIRVAVLLTDGISHPRNPDIFSAVAEAKNQGIKFFTLGITRAANEPTNIAQLRLVSSSPASRFLHNLQDEDIVEKIVAEITSLADQGCPLAQKCACEKGERGLSGPAGKKGRPGEDGAPGVKGEKGEYGISGLPGQEGPEGKPGYKGEKGERGECGTPGVKGDAGPVGLVGTRGPRGLQGLPGPPGDIGPEGIQGKQGERGPTGPPGIQGETGIGRPGPKGDIGFQGQPGPPGPRGIGELGPPGPQGPQGVQGSKGPTGEGLPGPKGDRGLPGPRGPRGQQGVGIKGDKGDFGPPGFPGPTGPTGVGIQGEKGVEGPRGPPGVRGLPGEGLPGPKGDQGLPGEQGAPGDRGIGEAGQKGEPGSAGIGGLPGLPGEDGAPGQKGEPGLPGLRGTEGAQGIGTQGEKGDQGQRGIRGLHGPPGIPGPSGPKGERGIPGQQGVQGQPGRSIPGPKGDVGSSGPPGPIGETGHGLPGPKGDHGHPGLPGPYGPKGEGLPGPMGPTGLPGLPGEPGPEGIGVPGPKGDIGFRGLPGLPGPPGEGIQGPPGNIGRPGPPGPRGPQGDGIQGPKGEPGSQGMTGPRGPPGDGLPGAKGDRGLTGEKGGKGGKGDLGDSGVPGEAGRAGAKGDPGLTREDIIKLIKEICGCGIKCKERPMELVFVIDSSESVGPENFEIIKDFVIRLVDRTTVGRNATRIGLVLYSLDVHLEFNLARYATKQDIRHAIRKIPYMGEGTYTGTAIRKATQEAFLNARRGVSKVAIVITDGQTDKREPVKLDLAVREAHAANIEMYALGIVNASDPTQAEFLQELNLIASDPDDEHMYLIDDFNTLPALESKLVSQFCEDENGALIYNHITNGHWNGNSGHGDSVGDNNGQNSNSNRGFGKSGISSSDKEEIQNRRRHNSRSRGDTFTLPISADPLPVQVEEDDEGEDLDIRAQARISSIATLINKTSVVSVREGSISNDVVLSSSSSSSNTSTSSASTLSLNSNKLQSTVLPQEVSPLDPRCSLSLNQGPCRNYTIHWYYDKQANSCAQFWYGGCGGNDNRYETEEECKKTCVVSLRRGG

Target ORF information:

RefSeq Version	XM_011609969.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609969.2

ORF Insert Sequence:

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAC 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGCTCAGCTA 
CATGAGGAGT GTGGTCTTGA GATCTCCTTC CTGTTGGACA GCTCTGAGAG CGCAAAGGAC 
AACCATGAGC AGGAGAAACA GTTTGTCATG AAGGTGGTGG ACAGACTCCA GGGGAAAAAG 
CTGCACACAG GCCGCAGCCT GAACCTGAGG GTGGCTCTGT TGCAGTACAG CAGTCACGTC 
ATCACGGAGC AAACCTTTAA GGACTGGAGG GGCACGGAGA ACTTCAAGGC TCGTGTAACT 
CCCATCATCT ACATCGGGCA CGGCACCTAC ACCACCTACG CCATCACCAA CATGACCAAG 
ATCTACCGGG AGGAGTCCAG TCCGGGCAGC ATCAGAGTGG CCGTGCTGCT CACAGATGGC 
ATTTCCCACC CGAGGAATCC GGATATTTTC TCTGCTGTGG CGGAAGCGAA AAACCAGGGC 
ATCAAATTCT TCACCCTGGG CATCACCCGC GCAGCCAACG AGCCCACCAA CATAGCCCAG 
CTCCGTCTTG TCTCCAGCTC CCCGGCCTCC CGCTTCCTCC ATAACCTGCA GGATGAAGAC 
ATTGTGGAGA AAATAGTCGC GGAGATTACC AGTTTGGCTG ATCAAGGATG CCCTTTGGCT 
CAGAAATGTG CTTGTGAGAA AGGAGAACGG GGCCTAAGTG GGCCTGCGGG CAAAAAAGGT 
CGGCCTGGAG AGGACGGAGC TCCAGGTGTT AAAGGGGAAA AGGGAGAATA TGGAATAAGT 
GGTCTACCTG GACAAGAGGG TCCTGAGGGT AAACCAGGTT ACAAGGGAGA GAAGGGAGAG 
AGAGGTGAGT GTGGTACTCC AGGAGTAAAA GGAGACGCGG GTCCTGTTGG TTTGGTCGGA 
ACGAGGGGAC CTCGGGGTTT GCAGGGGTTA CCAGGACCAC CAGGGGACAT TGGACCTGAG 
GGAATTCAGG GAAAGCAGGG CGAACGTGGC CCAACTGGCC CTCCAGGAAT TCAGGGGGAA 
ACTGGAATTG GACGTCCTGG TCCAAAGGGT GACATTGGAT TCCAAGGTCA GCCCGGTCCC 
CCCGGTCCTC GTGGAATAGG TGAACTAGGT CCTCCTGGTC CTCAAGGGCC GCAAGGTGTC 
CAAGGCAGTA AAGGACCCAC TGGTGAAGGT CTGCCTGGAC CAAAGGGGGA TCGGGGGCTC 
CCTGGACCGA GGGGGCCGAG GGGACAACAA GGAGTGGGAA TTAAAGGAGA TAAGGGGGAC 
TTTGGGCCTC CTGGTTTTCC AGGGCCAACT GGGCCAACAG GAGTTGGGAT ACAAGGAGAA 
AAGGGAGTTG AGGGTCCAAG AGGGCCACCA GGAGTCAGAG GACTTCCAGG CGAAGGTCTT 
CCTGGTCCTA AGGGAGATCA AGGTTTACCA GGAGAGCAAG GAGCTCCGGG AGATAGAGGT 
ATTGGTGAAG CCGGTCAAAA GGGAGAACCT GGGTCAGCTG GAATCGGAGG CCTCCCTGGT 
CTTCCAGGAG AGGATGGAGC ACCAGGGCAG AAGGGGGAGC CTGGTTTGCC TGGCTTAAGG 
GGAACCGAGG GAGCTCAAGG AATTGGTACT CAAGGGGAAA AGGGTGACCA GGGTCAAAGG 
GGCATTCGTG GATTACATGG GCCTCCTGGA ATCCCAGGAC CCTCTGGTCC AAAGGGTGAA 
CGTGGGATAC CAGGCCAGCA GGGCGTGCAG GGACAGCCAG GAAGGTCCAT ACCTGGTCCA 
AAGGGTGATG TTGGGTCTTC TGGTCCCCCT GGTCCAATTG GGGAGACAGG CCATGGATTA 
CCTGGTCCAA AGGGTGATCA TGGTCATCCA GGTTTACCAG GACCATATGG TCCCAAAGGT 
GAAGGTCTTC CTGGCCCTAT GGGTCCTACG GGTTTGCCAG GTTTACCAGG TGAGCCCGGT 
CCTGAAGGAA TTGGAGTTCC TGGTCCGAAG GGTGATATTG GCTTCAGAGG ATTGCCAGGA 
TTGCCTGGAC CACCTGGGGA GGGCATTCAA GGACCACCTG GTAATATTGG AAGGCCAGGG 
CCTCCTGGTC CAAGGGGACC ACAAGGAGAT GGTATTCAAG GCCCAAAGGG TGAGCCAGGA 
TCTCAGGGTA TGACAGGTCC CAGAGGGCCG CCGGGAGATG GACTCCCTGG AGCTAAGGGT 
GATCGGGGAT TAACTGGTGA AAAGGGGGGA AAAGGTGGAA AAGGAGACCT GGGAGATTCT 
GGAGTGCCTG GTGAAGCAGG AAGGGCGGGA GCAAAAGGAG ATCCAGGCCT CACCAGAGAG 
GACATCATTA AGCTCATTAA AGAAATCTGT GGATGTGGCA TCAAATGCAA AGAAAGACCG 
ATGGAGCTGG TGTTCGTCAT TGACAGCTCA GAGAGTGTTG GTCCAGAGAA TTTTGAAATC 
ATCAAGGACT TTGTCATCAG GCTGGTGGAC CGTACCACAG TTGGACGTAA CGCCACCAGA 
ATTGGACTAG TCCTCTACAG TCTGGATGTC CATTTAGAGT TCAACTTGGC TCGTTACGCG 
ACCAAGCAGG ACATCAGGCA CGCCATCAGA AAAATCCCTT ATATGGGCGA GGGCACCTAC 
ACTGGCACGG CAATCAGGAA GGCAACTCAG GAGGCTTTCT TAAACGCTCG GCGTGGCGTC 
AGTAAAGTGG CTATTGTCAT CACAGATGGT CAGACAGACA AACGAGAGCC AGTCAAGCTG 
GACCTGGCTG TGAGAGAAGC CCACGCTGCA AACATTGAGA TGTATGCCCT GGGCATTGTC 
AACGCTTCGG ATCCCACACA GGCCGAGTTC CTGCAAGAAC TCAACCTCAT CGCCTCTGAC 
CCTGACGATG AACACATGTA CCTCATCGAT GACTTCAATA CTCTACCAGC ACTGGAATCT 
AAATTGGTCA GCCAGTTCTG TGAGGATGAA AATGGAGCCC TGATTTACAA CCACATAACA 
AATGGACACT GGAACGGCAA CAGTGGACAT GGGGACAGTG TTGGTGACAA TAATGGCCAG 
AACAGTAACA GCAACAGAGG ATTTGGAAAG AGCGGGATCA GTTCCAGCGA TAAAGAGGAG 
ATCCAAAATC GGAGACGCCA CAACAGTCGA AGCCGTGGAG ATACTTTCAC ACTGCCCATA 
AGTGCCGATC CTCTTCCTGT GCAGGTGGAG GAGGATGATG AAGGTGAAGA TTTAGACATT 
AGAGCCCAAG CCCGCATCAG TAGTATTGCA ACATTAATTA ACAAAACATC TGTAGTATCT 
GTCAGAGAAG GCTCCATCTC TAATGATGTG GTTTTATCAT CTTCATCGTC TTCATCTAAC 
ACTTCAACCT CATCTGCATC AACTTTATCT TTAAACTCTA ACAAGTTACA GTCAACAGTC 
CTACCACAAG AGGTTTCTCC CCTTGATCCA CGCTGTAGTC TGAGTTTGAA TCAAGGTCCC 
TGTCGAAACT ACACCATCCA CTGGTACTAT GACAAGCAGG CCAACTCCTG CGCACAGTTT 
TGGTACGGAG GCTGTGGAGG AAATGATAAT CGTTATGAAA CAGAGGAAGA ATGCAAGAAA 
ACCTGTGTAG TAAGCCTTCG CAGAGGAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101068515 ( XM_011609975.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011608277.2 Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo43049
Clone ID Related Accession (Same CDS sequence)	XM_011609975.2
Accession Version	XM_011609975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XXVIII) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011609975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAC 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTCGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGTCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATCA TGGTCATCCA GGTTTACCAG GACCATATGG TCCCAAAGGT 
GAAGGTCTTC CTGGCCCTAT GGGTCCTACG GGTTTGCCAG GTTTACCAGG TGAGCCCGGT 
CCTGAAGGAA TTGGAGTTCC TGGTCCGAAG GGTGATATTG GCTTCAGAGG ATTGCCAGGA 
TTGCCTGGAC CACCTGGGGA GGGCATTCAA GGACCACCTG GTAATATTGG AAGGCCAGGG 
CCTCCTGGTC CAAGGGGACC ACAAGGAGAT GGTATTCAAG GCCCAAAGGG TGAGCCAGGA 
TCTCAGGGTA TGACAGGTCC CAGAGGGCCG CCGGGAGATG GACTCCCTGG AGCTAAGGGT 
GATCGGGGAT TAACTGGTGA AAAGGGGGGA AAAGGTGGAA AAGGAGACCT GGGAGATTCT 
GGAGTGCCTG GTGAAGCAGG AAGGGCGGGA GCAAAAGGAG ATCCAGGCCT CACCAGAGAG 
GACATCATTA AGCTCATTAA AGAAATCTGT GGATGTGGCA TCAAATGCAA AGAAAGACCG 
ATGGAGCTGG TGTTCGTCAT TGACAGCTCA GAGAGTGTTG GTCCAGAGAA TTTTGAAATC 
ATCAAGGACT TTGTCATCAG GCTGGTGGAC CGTACCACAG TTGGACGTAA CGCCACCAGA 
ATTGGACTAG TCCTCTACAG TCTGGATGTC CATTTAGAGT TCAACTTGGC TCGTTACGCG 
ACCAAGCAGG ACATCAGGCA CGCCATCAGA AAAATCCCTT ATATGGGCGA GGGCACCTAC 
ACTGGCACGG CAATCAGGAA GGCAACTCAG GAGGCTTTCT TAAACGCTCG GCGTGGCGTC 
AGTAAAGTGG CTATTGTCAT CACAGATGGT CAGACAGACA AACGAGAGCC AGTCAAGCTG 
GACCTGGCTG TGAGAGAAGC CCACGCTGCA AACATTGAGA TGTATGCCCT GGGCATTGTC 
AACGCTTCGG ATCCCACACA GGCCGAGTTC CTGCAAGAAC TCAACCTCAT CGCCTCTGAC 
CCTGACGATG AACACATGTA CCTCATCGAT GACTTCAATA CTCTACCAGC ACTGGAATCT 
AAATTGGTCA GCCAGTTCTG TGAGGATGAA AATGGAGCCC TGATTTACAA CCACATAACA 
AATGGACACT GGAACGGCAA CAGTGGACAT GGGGACAGTG TTGGTGACAA TAATGGCCAG 
AACAGTAACA GCAACAGAGG ATTTGGAAAG AGCGGGATCA GTTCCAGCGA TAAAGAGGAG 
ATCCAAAATC GGAGACGCCA CAACAGTCGA AGCCGTGGAG ATACTTTCAC ACTGCCCATA 
AGTGCCGATC CTCTTCCTGT GCAGGTGGAG GAGGATGATG AAGGTGAAGA TTTAGACATT 
AGAGCCCAAG CCCGCATCAG TAGTATTGCA ACATTAATTA ACAAAACATC TGTAGTATCT 
GTCAGAGAAG GCTCCATCTC TAATGATGTG GTTTTATCAT CTTCATCGTC TTCATCTAAC 
ACTTCAACCT CATCTGCATC AACTTTATCT TTAAACTCTA ACAAGTTACA GTCAACAGTC 
CTACCACAAG AGGTTTCTCC CCTTGATCCA CGCTGTAGTC TGAGTTTGAA TCAAGGTCCC 
TGTCGAAACT ACACCATCCA CTGGTACTAT GACAAGCAGG CCAACTCCTG CGCACAGTTT 
TGGTACGGAG GCTGTGGAGG AAATGATAAT CGTTATGAAA CAGAGGAAGA ATGCAAGAAA 
ACCTGTGTAG TAAGCCTTCG CAGAGGAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011608277.2
CDS	174..3746
Translation	MLSCTWVLLVTGLSCVWTQHTLEEKKPVNKVLSAGTHGRQAQLHEECGLEISFLLDSSESAKDNHEQEKQFVMKVVDRLQGKKLHTGRSLNLRVALLQYSSHVITEQTFKDWRGTENFKARVTPIIYIGHGTYTTYAITNMTKIYREESSPGSIRVAVLLTDGISHPRNPDIFSAVAEAKNQGIKFFTLGITRAANEPTNIAQLRLVSSSPASRFLHNLQDEDIVEKIVAEITSLADQGCPLAQKCACEKGERGLSGPAGKKGRPGEDGAPGVKGEKGEYGISGLPGQEGPEGKPGYKGEKGERGECGTPGVKGDAGPVGLVGTRGPRGLQGLPGPPGDIGPEGIQGKQGERGPTGPPGIQGETGIGRPGPKGDIGFQGQPGPPGPRGIGELGPPGPQGPQGVQGSKGPTGEGLPGPKGDRGLPGPRGPRGQQGVGIKGDKGDFGPPGFPGPTGPTGVGIQGEKGVEGPRGPPGVRGLPGEGLPGPKGDQGLPGEQGAPGDRGIGEAGQKGEPGSAGIGGLPGLPGEDGAPGQKGEPGLPGLRGTEGAQGIGTQGEKGDQGQRGIRGLHGPPGIPGPSGPKGERGIPGQQGVQGQPGRSIPGPKGDHGHPGLPGPYGPKGEGLPGPMGPTGLPGLPGEPGPEGIGVPGPKGDIGFRGLPGLPGPPGEGIQGPPGNIGRPGPPGPRGPQGDGIQGPKGEPGSQGMTGPRGPPGDGLPGAKGDRGLTGEKGGKGGKGDLGDSGVPGEAGRAGAKGDPGLTREDIIKLIKEICGCGIKCKERPMELVFVIDSSESVGPENFEIIKDFVIRLVDRTTVGRNATRIGLVLYSLDVHLEFNLARYATKQDIRHAIRKIPYMGEGTYTGTAIRKATQEAFLNARRGVSKVAIVITDGQTDKREPVKLDLAVREAHAANIEMYALGIVNASDPTQAEFLQELNLIASDPDDEHMYLIDDFNTLPALESKLVSQFCEDENGALIYNHITNGHWNGNSGHGDSVGDNNGQNSNSNRGFGKSGISSSDKEEIQNRRRHNSRSRGDTFTLPISADPLPVQVEEDDEGEDLDIRAQARISSIATLINKTSVVSVREGSISNDVVLSSSSSSSNTSTSSASTLSLNSNKLQSTVLPQEVSPLDPRCSLSLNQGPCRNYTIHWYYDKQANSCAQFWYGGCGGNDNRYETEEECKKTCVVSLRRGG

Target ORF information:

RefSeq Version	XM_011609975.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XXVIII) chain-like (LOC101068515), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609975.2

ORF Insert Sequence:

ATGCTCTCCT GCACGTGGGT GCTGCTGGTC ACAGGACTGA GCTGCGTCTG GACACAACAC 
ACTTTGGAAG AGAAGAAACC TGTGAACAAA GTTCTGAGTG CAGGAACTCA TGGTAGGCAA 
GCTCAGCTAC ATGAGGAGTG TGGTCTTGAG ATCTCCTTCC TGTTGGACAG CTCTGAGAGC 
GCAAAGGACA ACCATGAGCA GGAGAAACAG TTTGTCATGA AGGTGGTGGA CAGACTCCAG 
GGGAAAAAGC TGCACACAGG CCGCAGCCTG AACCTGAGGG TGGCTCTGTT GCAGTACAGC 
AGTCACGTCA TCACGGAGCA AACCTTTAAG GACTGGAGGG GCACGGAGAA CTTCAAGGCT 
CGTGTAACTC CCATCATCTA CATCGGGCAC GGCACCTACA CCACCTACGC CATCACCAAC 
ATGACCAAGA TCTACCGGGA GGAGTCCAGT CCGGGCAGCA TCAGAGTGGC CGTGCTGCTC 
ACAGATGGCA TTTCCCACCC GAGGAATCCG GATATTTTCT CTGCTGTGGC GGAAGCGAAA 
AACCAGGGCA TCAAATTCTT CACCCTGGGC ATCACCCGCG CAGCCAACGA GCCCACCAAC 
ATAGCCCAGC TCCGTCTTGT CTCCAGCTCC CCGGCCTCCC GCTTCCTCCA TAACCTGCAG 
GATGAAGACA TTGTGGAGAA AATAGTCGCG GAGATTACCA GTTTGGCTGA TCAAGGATGC 
CCTTTGGCTC AGAAATGTGC TTGTGAGAAA GGAGAACGGG GCCTAAGTGG GCCTGCGGGC 
AAAAAAGGTC GGCCTGGAGA GGACGGAGCT CCAGGTGTTA AAGGGGAAAA GGGAGAATAT 
GGAATAAGTG GTCTACCTGG ACAAGAGGGT CCTGAGGGTA AACCAGGTTA CAAGGGAGAG 
AAGGGAGAGA GAGGTGAGTG TGGTACTCCA GGAGTAAAAG GAGACGCGGG TCCTGTTGGT 
TTGGTCGGAA CGAGGGGACC TCGGGGTTTG CAGGGGTTAC CAGGACCACC AGGGGACATT 
GGACCTGAGG GAATTCAGGG AAAGCAGGGC GAACGTGGCC CAACTGGCCC TCCAGGAATT 
CAGGGGGAAA CTGGAATTGG ACGTCCTGGT CCAAAGGGTG ACATTGGATT CCAAGGTCAG 
CCCGGTCCCC CCGGTCCTCG TGGAATAGGT GAACTAGGTC CTCCTGGTCC TCAAGGGCCG 
CAAGGTGTCC AAGGCAGTAA AGGACCCACT GGTGAAGGTC TGCCTGGACC AAAGGGGGAT 
CGGGGGCTCC CTGGACCGAG GGGGCCGAGG GGACAACAAG GAGTGGGAAT TAAAGGAGAT 
AAGGGGGACT TTGGGCCTCC TGGTTTTCCA GGGCCAACTG GGCCAACAGG AGTTGGGATA 
CAAGGAGAAA AGGGAGTTGA GGGTCCAAGA GGGCCACCAG GAGTCAGAGG ACTTCCAGGC 
GAAGGTCTTC CTGGTCCTAA GGGAGATCAA GGTTTACCAG GAGAGCAAGG AGCTCCGGGA 
GATAGAGGTA TTGGTGAAGC CGGTCAAAAG GGAGAACCTG GGTCAGCTGG AATCGGAGGC 
CTCCCTGGTC TTCCAGGAGA GGATGGAGCA CCAGGGCAGA AGGGGGAGCC TGGTTTGCCT 
GGCTTAAGGG GAACCGAGGG AGCTCAAGGA ATTGGTACTC AAGGGGAAAA GGGTGACCAG 
GGTCAAAGGG GCATTCGTGG ATTACATGGG CCTCCTGGAA TCCCAGGACC CTCTGGTCCA 
AAGGGTGAAC GTGGGATACC AGGCCAGCAG GGCGTGCAGG GACAGCCAGG AAGGTCCATA 
CCTGGTCCAA AGGGTGATCA TGGTCATCCA GGTTTACCAG GACCATATGG TCCCAAAGGT 
GAAGGTCTTC CTGGCCCTAT GGGTCCTACG GGTTTGCCAG GTTTACCAGG TGAGCCCGGT 
CCTGAAGGAA TTGGAGTTCC TGGTCCGAAG GGTGATATTG GCTTCAGAGG ATTGCCAGGA 
TTGCCTGGAC CACCTGGGGA GGGCATTCAA GGACCACCTG GTAATATTGG AAGGCCAGGG 
CCTCCTGGTC CAAGGGGACC ACAAGGAGAT GGTATTCAAG GCCCAAAGGG TGAGCCAGGA 
TCTCAGGGTA TGACAGGTCC CAGAGGGCCG CCGGGAGATG GACTCCCTGG AGCTAAGGGT 
GATCGGGGAT TAACTGGTGA AAAGGGGGGA AAAGGTGGAA AAGGAGACCT GGGAGATTCT 
GGAGTGCCTG GTGAAGCAGG AAGGGCGGGA GCAAAAGGAG ATCCAGGCCT CACCAGAGAG 
GACATCATTA AGCTCATTAA AGAAATCTGT GGATGTGGCA TCAAATGCAA AGAAAGACCG 
ATGGAGCTGG TGTTCGTCAT TGACAGCTCA GAGAGTGTTG GTCCAGAGAA TTTTGAAATC 
ATCAAGGACT TTGTCATCAG GCTGGTGGAC CGTACCACAG TTGGACGTAA CGCCACCAGA 
ATTGGACTAG TCCTCTACAG TCTGGATGTC CATTTAGAGT TCAACTTGGC TCGTTACGCG 
ACCAAGCAGG ACATCAGGCA CGCCATCAGA AAAATCCCTT ATATGGGCGA GGGCACCTAC 
ACTGGCACGG CAATCAGGAA GGCAACTCAG GAGGCTTTCT TAAACGCTCG GCGTGGCGTC 
AGTAAAGTGG CTATTGTCAT CACAGATGGT CAGACAGACA AACGAGAGCC AGTCAAGCTG 
GACCTGGCTG TGAGAGAAGC CCACGCTGCA AACATTGAGA TGTATGCCCT GGGCATTGTC 
AACGCTTCGG ATCCCACACA GGCCGAGTTC CTGCAAGAAC TCAACCTCAT CGCCTCTGAC 
CCTGACGATG AACACATGTA CCTCATCGAT GACTTCAATA CTCTACCAGC ACTGGAATCT 
AAATTGGTCA GCCAGTTCTG TGAGGATGAA AATGGAGCCC TGATTTACAA CCACATAACA 
AATGGACACT GGAACGGCAA CAGTGGACAT GGGGACAGTG TTGGTGACAA TAATGGCCAG 
AACAGTAACA GCAACAGAGG ATTTGGAAAG AGCGGGATCA GTTCCAGCGA TAAAGAGGAG 
ATCCAAAATC GGAGACGCCA CAACAGTCGA AGCCGTGGAG ATACTTTCAC ACTGCCCATA 
AGTGCCGATC CTCTTCCTGT GCAGGTGGAG GAGGATGATG AAGGTGAAGA TTTAGACATT 
AGAGCCCAAG CCCGCATCAG TAGTATTGCA ACATTAATTA ACAAAACATC TGTAGTATCT 
GTCAGAGAAG GCTCCATCTC TAATGATGTG GTTTTATCAT CTTCATCGTC TTCATCTAAC 
ACTTCAACCT CATCTGCATC AACTTTATCT TTAAACTCTA ACAAGTTACA GTCAACAGTC 
CTACCACAAG AGGTTTCTCC CCTTGATCCA CGCTGTAGTC TGAGTTTGAA TCAAGGTCCC 
TGTCGAAACT ACACCATCCA CTGGTACTAT GACAAGCAGG CCAACTCCTG CGCACAGTTT 
TGGTACGGAG GCTGTGGAGG AAATGATAAT CGTTATGAAA CAGAGGAAGA ATGCAAGAAA 
ACCTGTGTAG TAAGCCTTCG CAGAGGAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101068515 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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