brca1 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	brca1
Entrez Gene ID	101064828
Full Name	BRCA1, DNA repair associated
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003961258.3	XP_003961307.3	breast cancer type 1 susceptibility protein
XM_011602400.1	XP_011600702.1	breast cancer type 1 susceptibility protein isoform X2
XM_003961258.2	XP_003961307.2	breast cancer type 1 susceptibility protein isoform X1

Pathways from BioSystems
Gene Ontology

The following brca1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brca1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo21405	XM_003961258.2 Latest version!	Takifugu rubripes breast cancer 1, early onset (brca1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo21406	XM_011602400.1 Latest version!	Takifugu rubripes breast cancer 1, early onset (brca1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo42595	XM_003961258.3 Latest version!	Takifugu rubripes BRCA1 DNA repair associated (brca1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brca1 ( XM_003961258.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003961307.2 Takifugu rubripes breast cancer 1, early onset (brca1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo21405
Clone ID Related Accession (Same CDS sequence)	XM_003961258.2
Accession Version	XM_003961258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003961258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAATA AAGGAGAAGA AGATTTTAGT TGCGGGAAAA CTGAAGCTTG TCATTTCTGG 
AATTGTACGC TTCATCTACG AGAAAATAAG GACATTAAAA TAATCACCAT GGAAGCAGCA 
AAAGCCGCAG ATGTTAAGAA AGGGATTTCA GTGCTTTGGG AGAGTCTACA GTGTCCAATA 
TGCTTGGATA TATTGACTGC ACCTGTATCT ACCAAGTGTG ACCATCAATT TTGCAAATTC 
TGTATCTCGA AACTTTTAAG CAACACCAAA CAAAACAAGG CTAACTGTCC AGTATGCAAA 
TCCAAGATAA CCAAAAGGAG CTTACAAGAG AGCCCTGGGT TTCAAAGACT TGTCACAGGA 
TTACAGGGAA TGATTCTGGC ATATGAAAAT GACACAGGCA CAAACTATTT TACTGGGCTG 
TCCAGGCAAA CACAGCAACC ACATGTTGCA GATATCAAAA ATGCTCAACA TCATAAGGAA 
CAATCAGATG AAAATGCTTC CTGTACGGAA GATGATAATG AAGATCTTCC AAAGTCTTAT 
TCTTCAACTA GAGCAGCCCA GGATGGATTT GCAAGGCTTA TGGGCCTAGA AGACACAAGT 
CCTCTGACTA CAGAAATCGA TGGCCTGGAC AGTGGTCTTG GAGAGGCCCC GCCAACTTGT 
GACAAAAAAA TGTACAGCCC AAGAAAGTTG GAGAAGGTTT CCTTTCAGCC AGCATTAATA 
CCAGATAAGG ATGAACACCC ACATTTACAA ACACCTTCAA AGAAGAAGCA TAAAAAGGTT 
TTAGAGCCTG ATAAGATTCT CGATCAGAAA CAGAAGAAAA GTCTAGAGAA AGTTGCAGAG 
TGGCTCATGA AAGTTCCATC CGAGCAAAGC CTGGAATTGG AGAACCTGGA TGAAGATGGC 
GATGACTCCG ACAGCTGCTC ATCCACCTCA ACAATAGATC TTGGGCTACT CCGCTGTGAA 
TCAAATCCTA TGAGAGCTCG TGGAAAAGCC CTGGAAGATC AGGTGTTTGG GGCGATTTAT 
AAAAGACAGC GAAGAGGGAA GGGAATCGTA AAACCGATAG AAGCGGCACT CGAAATGGTT 
TGTTTAAATC ATTCTGACAA AAATACCTCT GAAGATAAAA GCAGAGAAGA TAAGGAAGAG 
GTGCACTCGA TAAGAGAGCA AGAACAAAAC ACCAGTAGCA ACATATTAAA AGAAGAGATT 
GCAGTCTTGG AGGATTGTGG AGGAATCGTT GAGCCAACTC ACATGTCAGA AAATGATCAA 
AACAACAAAG ATGAAGTACC TCATCTAGTT TCTGATATTG GACAACAGCA ACCAGAAACC 
ATTGCAAAGA GAACACGGTC TTCTCTGCAG CAAGTCGACT GTGATTTGTT GAAGTGCACA 
CAAAAAAAGC CAGAAGATAG TGGGAAAAAG AAAATTGCCC AGAGAGGAAG TAAAGGCATC 
AAGTCTGAAA AATCAAAGTC TGCCAGAATG TCAAAACCGC TTGTTTTGGT TACGGTTGAA 
AAGCTAGACA CTAGTCCCAA GATTAGACCG CAGTCAGAAA AAGTTGAAGT TCACATTGAA 
AACTATCCCA GCAGTGGTGA CCAAGAAGTT CCTTTAGGTA GATGTACCAG GAAAAGTAGA 
AGACTTCAGG TCTTCACTAA GGAAGATGAC AAGAAAGAAA CATCAAGATT CAATGTCCCA 
GAAAAACAAC ACAATTCAAA ATACCCCAAC TTTGAATGTG AAATACTGGA TAATATCAAA 
TCACAAAATT GTAAATACCA AACACAACAA GCAAACAGAA ATGGATGTAT TTATAATCAA 
GGTATCCAAG AAATAGAAAA CATGGATTCT GGTGAGAGAG CATCTTCTTT GAGATCAGAA 
GAAGATACTA AAACTTCTCT TTCTGAAGTC CAAAATACCG AAATTTTGTC TGAAACTCCT 
CGCTGCGTCG CTGTGTTCCC AAGTTGTGCT GAACTAGCTC CGGCAGAACC AACTAATCAA 
CCCCCAAATA CTGTCCAGTT GGTGACTGAA CTCGCTGAAA AGGAACGCGA GCAACAGAAC 
GATAGTGAGC AGGACACAGA ACAGTTGGTC AGGAGCTTTA AAGCTACTAA AAGAAAATCT 
TTCCATCTCG GTACTGGTCC AAATATGAAA AGGAGCCATT TATTAGTTCA TGAAAACACC 
CAAAGTCCAG GAACTGAAGA GAACGAAAAT TATTATGAAT CTTTGCCAAA ACAAGTGCAG 
CCAGTGATGG CTAAAATAAC CAACAGTCAG GTTTTAATTG ACAGTCAAAA CATGTCTGGT 
AGTGACTTGA TCTCACCGTC CTGCTCACCC AGCCTGACGA GAAAAGCCTT GGGCCTTCAA 
AGCAGATGTT CAGTGGAAGT TGGAAATTGT GTCCTCGGTA ATAGTGCCAG TAGCCCCCTT 
TCTCCTAATA ACGAATCGAA GCATGTAGTA CAAAGTTCCT CTCCTCTCAT ACTAACAAAG 
GGAAACTCGA CAATCTGCTT TGCAACTGAA AAACCTTCTC AAATTTCAGA GAGCAAAACT 
GATTTTATAA TGATGAAGGA GACACGAGGT AATACTTCAC TGCACAGTTT TAATGGCAGC 
ACTGCTGAGG AACTTTTAGA TGCACAGTCG TCATTAACCC CGGATGGCCT GGGAATGCCA 
GTCCCAGAGG TGACATACAG CCAAGGGAGC AGAGAGCTCA GCAGGTGCAG GAAAAGGACA 
AAGTCTCAGA AGCTGGACTC TTCATCAGAT TGTACAGAAG AGGAATTTCC ATGTTTTTCA 
AAAATCCTCA ACAAAACTGC CCCCCCTGGT GCTAATCCTC CAGCAAGTCC CAGCCTTGAT 
TGTGTTAATT CCAGCCAAGC ATCTGTGGAC TTGTTTGACA TGCCAAATGA GTGTGCCATA 
AATAATGTCG AGTCATCGCA ATTTTCAAGC GAGGTCCTCG TTACGCAGCA AAAAATAGAG 
ATGAAGAAGG AGCTGGTGAG GTTGGAAAAG TTGATGGCAC TGGTGACAGA GGTGCTACAC 
GAGAAGGAAA CCAGCCCAGC AAAAAACATC CAGAATAAAA CCAACCTGAG CAGCAAAATA 
ACAGGCTCAG ATGCTGACAA ACTGCGCCCG AATGTCCAGG ATGAAGATTT TAACCAAGAA 
ACATTCCCAG AAGAAGCACT AGAACCCAAC ACAAGATATT CTGATGCGCC TCCTTCTGGT 
TCCAAGCACA GCAGGACGAC AGAGATGAAC TCCAGAACAT CCAACACAGT TGGGTTAAGT 
TCAGCTGCAA AGACTGATGG TTCCCCATCA GCCGGCCACG AAGACAAAGA AAATAACAAG 
CCTGAGGGAG ACAGAAGCCA AGCCAAGATG CTCCTGGTGA CGTCTGGACT GGGCGCCAGT 
GAACAGATAA CAGTGAAAAA GTTCGCTAAG CGGATTGGCG CCGTTGTGGT CTCCCAGGTG 
ACACCAGAGG TGACTCACAT TGTCATGCAC ACAGATGAAC AACTGGTGTG TGAGCGCACA 
CTGAAGTACT TTCTTGGAAT TGCGGGCCGG AAGTGGGTGG TGAGCTTCCA ATGGATTTCG 
GAGTGCTTCA AACAAAAGAA ACTTTTAAAT GAGAGTACCT TTGAAGTGAG AGGTGATGTA 
GTTAATGGAT CCAACCACCA GGGCCCAATG AAAGCTCGAA CAACAGGAGA CAACAATTTG 
CTGATGAAAG ACTTCAATAT CTGTTTTCAG GGGCCTTTTA CAGACATGAC CACAGCTGAA 
ATGGAGTTGA TGGTTGAGCT GTGTGGAGCG ACTGTTGTCA AAGATCCACT TCTGCTCCAC 
AGTAAACCGA CTCATCATCA GCTGATCATC GTTCAGTCCG GATCAGAATC CTACAAGAGC 
CTCTCTAGAA AGGCTACAGT TGTGACTCGG GGGTGGCTGT TGGACACCGT GGCAACCTAC 
ACCATCCAAG ACTTCAGGAC CTACAGAGCT GACCTGAGAG CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961307.2
CDS	56..4003
Translation	MLNKGEEDFSCGKTEACHFWNCTLHLRENKDIKIITMEAAKAADVKKGISVLWESLQCPICLDILTAPVSTKCDHQFCKFCISKLLSNTKQNKANCPVCKSKITKRSLQESPGFQRLVTGLQGMILAYENDTGTNYFTGLSRQTQQPHVADIKNAQHHKEQSDENASCTEDDNEDLPKSYSSTRAAQDGFARLMGLEDTSPLTTEIDGLDSGLGEAPPTCDKKMYSPRKLEKVSFQPALIPDKDEHPHLQTPSKKKHKKVLEPDKILDQKQKKSLEKVAEWLMKVPSEQSLELENLDEDGDDSDSCSSTSTIDLGLLRCESNPMRARGKALEDQVFGAIYKRQRRGKGIVKPIEAALEMVCLNHSDKNTSEDKSREDKEEVHSIREQEQNTSSNILKEEIAVLEDCGGIVEPTHMSENDQNNKDEVPHLVSDIGQQQPETIAKRTRSSLQQVDCDLLKCTQKKPEDSGKKKIAQRGSKGIKSEKSKSARMSKPLVLVTVEKLDTSPKIRPQSEKVEVHIENYPSSGDQEVPLGRCTRKSRRLQVFTKEDDKKETSRFNVPEKQHNSKYPNFECEILDNIKSQNCKYQTQQANRNGCIYNQGIQEIENMDSGERASSLRSEEDTKTSLSEVQNTEILSETPRCVAVFPSCAELAPAEPTNQPPNTVQLVTELAEKEREQQNDSEQDTEQLVRSFKATKRKSFHLGTGPNMKRSHLLVHENTQSPGTEENENYYESLPKQVQPVMAKITNSQVLIDSQNMSGSDLISPSCSPSLTRKALGLQSRCSVEVGNCVLGNSASSPLSPNNESKHVVQSSSPLILTKGNSTICFATEKPSQISESKTDFIMMKETRGNTSLHSFNGSTAEELLDAQSSLTPDGLGMPVPEVTYSQGSRELSRCRKRTKSQKLDSSSDCTEEEFPCFSKILNKTAPPGANPPASPSLDCVNSSQASVDLFDMPNECAINNVESSQFSSEVLVTQQKIEMKKELVRLEKLMALVTEVLHEKETSPAKNIQNKTNLSSKITGSDADKLRPNVQDEDFNQETFPEEALEPNTRYSDAPPSGSKHSRTTEMNSRTSNTVGLSSAAKTDGSPSAGHEDKENNKPEGDRSQAKMLLVTSGLGASEQITVKKFAKRIGAVVVSQVTPEVTHIVMHTDEQLVCERTLKYFLGIAGRKWVVSFQWISECFKQKKLLNESTFEVRGDVVNGSNHQGPMKARTTGDNNLLMKDFNICFQGPFTDMTTAEMELMVELCGATVVKDPLLLHSKPTHHQLIIVQSGSESYKSLSRKATVVTRGWLLDTVATYTIQDFRTYRADLRAA

Target ORF information:

RefSeq Version	XM_003961258.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes breast cancer 1, early onset (brca1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961258.2

ORF Insert Sequence:

ATGTTGAATA AAGGAGAAGA AGATTTTAGT TGCGGGAAAA CTGAAGCTTG TCATTTCTGG 
AATTGTACGC TTCATCTACG AGAAAATAAG GACATTAAAA TAATCACCAT GGAAGCAGCA 
AAAGCCGCAG ATGTTAAGAA AGGGATTTCA GTGCTTTGGG AGAGTCTACA GTGTCCAATA 
TGCTTGGATA TATTGACTGC ACCTGTATCT ACCAAGTGTG ACCATCAATT TTGCAAATTC 
TGTATCTCGA AACTTTTAAG CAACACCAAA CAAAACAAGG CTAACTGTCC AGTATGCAAA 
TCCAAGATAA CCAAAAGGAG CTTACAAGAG AGCCCTGGGT TTCAAAGACT TGTCACAGGA 
TTACAGGGAA TGATTCTGGC ATATGAAAAT GACACAGGCA CAAACTATTT TACTGGGCTG 
TCCAGGCAAA CACAGCAACC ACATGTTGCA GATATCAAAA ATGCTCAACA TCATAAGGAA 
CAATCAGATG AAAATGCTTC CTGTACGGAA GATGATAATG AAGATCTTCC AAAGTCTTAT 
TCTTCAACTA GAGCAGCCCA GGATGGATTT GCAAGGCTTA TGGGCCTAGA AGACACAAGT 
CCTCTGACTA CAGAAATCGA TGGCCTGGAC AGTGGTCTTG GAGAGGCCCC GCCAACTTGT 
GACAAAAAAA TGTACAGCCC AAGAAAGTTG GAGAAGGTTT CCTTTCAGCC AGCATTAATA 
CCAGATAAGG ATGAACACCC ACATTTACAA ACACCTTCAA AGAAGAAGCA TAAAAAGGTT 
TTAGAGCCTG ATAAGATTCT CGATCAGAAA CAGAAGAAAA GTCTAGAGAA AGTTGCAGAG 
TGGCTCATGA AAGTTCCATC CGAGCAAAGC CTGGAATTGG AGAACCTGGA TGAAGATGGC 
GATGACTCCG ACAGCTGCTC ATCCACCTCA ACAATAGATC TTGGGCTACT CCGCTGTGAA 
TCAAATCCTA TGAGAGCTCG TGGAAAAGCC CTGGAAGATC AGGTGTTTGG GGCGATTTAT 
AAAAGACAGC GAAGAGGGAA GGGAATCGTA AAACCGATAG AAGCGGCACT CGAAATGGTT 
TGTTTAAATC ATTCTGACAA AAATACCTCT GAAGATAAAA GCAGAGAAGA TAAGGAAGAG 
GTGCACTCGA TAAGAGAGCA AGAACAAAAC ACCAGTAGCA ACATATTAAA AGAAGAGATT 
GCAGTCTTGG AGGATTGTGG AGGAATCGTT GAGCCAACTC ACATGTCAGA AAATGATCAA 
AACAACAAAG ATGAAGTACC TCATCTAGTT TCTGATATTG GACAACAGCA ACCAGAAACC 
ATTGCAAAGA GAACACGGTC TTCTCTGCAG CAAGTCGACT GTGATTTGTT GAAGTGCACA 
CAAAAAAAGC CAGAAGATAG TGGGAAAAAG AAAATTGCCC AGAGAGGAAG TAAAGGCATC 
AAGTCTGAAA AATCAAAGTC TGCCAGAATG TCAAAACCGC TTGTTTTGGT TACGGTTGAA 
AAGCTAGACA CTAGTCCCAA GATTAGACCG CAGTCAGAAA AAGTTGAAGT TCACATTGAA 
AACTATCCCA GCAGTGGTGA CCAAGAAGTT CCTTTAGGTA GATGTACCAG GAAAAGTAGA 
AGACTTCAGG TCTTCACTAA GGAAGATGAC AAGAAAGAAA CATCAAGATT CAATGTCCCA 
GAAAAACAAC ACAATTCAAA ATACCCCAAC TTTGAATGTG AAATACTGGA TAATATCAAA 
TCACAAAATT GTAAATACCA AACACAACAA GCAAACAGAA ATGGATGTAT TTATAATCAA 
GGTATCCAAG AAATAGAAAA CATGGATTCT GGTGAGAGAG CATCTTCTTT GAGATCAGAA 
GAAGATACTA AAACTTCTCT TTCTGAAGTC CAAAATACCG AAATTTTGTC TGAAACTCCT 
CGCTGCGTCG CTGTGTTCCC AAGTTGTGCT GAACTAGCTC CGGCAGAACC AACTAATCAA 
CCCCCAAATA CTGTCCAGTT GGTGACTGAA CTCGCTGAAA AGGAACGCGA GCAACAGAAC 
GATAGTGAGC AGGACACAGA ACAGTTGGTC AGGAGCTTTA AAGCTACTAA AAGAAAATCT 
TTCCATCTCG GTACTGGTCC AAATATGAAA AGGAGCCATT TATTAGTTCA TGAAAACACC 
CAAAGTCCAG GAACTGAAGA GAACGAAAAT TATTATGAAT CTTTGCCAAA ACAAGTGCAG 
CCAGTGATGG CTAAAATAAC CAACAGTCAG GTTTTAATTG ACAGTCAAAA CATGTCTGGT 
AGTGACTTGA TCTCACCGTC CTGCTCACCC AGCCTGACGA GAAAAGCCTT GGGCCTTCAA 
AGCAGATGTT CAGTGGAAGT TGGAAATTGT GTCCTCGGTA ATAGTGCCAG TAGCCCCCTT 
TCTCCTAATA ACGAATCGAA GCATGTAGTA CAAAGTTCCT CTCCTCTCAT ACTAACAAAG 
GGAAACTCGA CAATCTGCTT TGCAACTGAA AAACCTTCTC AAATTTCAGA GAGCAAAACT 
GATTTTATAA TGATGAAGGA GACACGAGGT AATACTTCAC TGCACAGTTT TAATGGCAGC 
ACTGCTGAGG AACTTTTAGA TGCACAGTCG TCATTAACCC CGGATGGCCT GGGAATGCCA 
GTCCCAGAGG TGACATACAG CCAAGGGAGC AGAGAGCTCA GCAGGTGCAG GAAAAGGACA 
AAGTCTCAGA AGCTGGACTC TTCATCAGAT TGTACAGAAG AGGAATTTCC ATGTTTTTCA 
AAAATCCTCA ACAAAACTGC CCCCCCTGGT GCTAATCCTC CAGCAAGTCC CAGCCTTGAT 
TGTGTTAATT CCAGCCAAGC ATCTGTGGAC TTGTTTGACA TGCCAAATGA GTGTGCCATA 
AATAATGTCG AGTCATCGCA ATTTTCAAGC GAGGTCCTCG TTACGCAGCA AAAAATAGAG 
ATGAAGAAGG AGCTGGTGAG GTTGGAAAAG TTGATGGCAC TGGTGACAGA GGTGCTACAC 
GAGAAGGAAA CCAGCCCAGC AAAAAACATC CAGAATAAAA CCAACCTGAG CAGCAAAATA 
ACAGGCTCAG ATGCTGACAA ACTGCGCCCG AATGTCCAGG ATGAAGATTT TAACCAAGAA 
ACATTCCCAG AAGAAGCACT AGAACCCAAC ACAAGATATT CTGATGCGCC TCCTTCTGGT 
TCCAAGCACA GCAGGACGAC AGAGATGAAC TCCAGAACAT CCAACACAGT TGGGTTAAGT 
TCAGCTGCAA AGACTGATGG TTCCCCATCA GCCGGCCACG AAGACAAAGA AAATAACAAG 
CCTGAGGGAG ACAGAAGCCA AGCCAAGATG CTCCTGGTGA CGTCTGGACT GGGCGCCAGT 
GAACAGATAA CAGTGAAAAA GTTCGCTAAG CGGATTGGCG CCGTTGTGGT CTCCCAGGTG 
ACACCAGAGG TGACTCACAT TGTCATGCAC ACAGATGAAC AACTGGTGTG TGAGCGCACA 
CTGAAGTACT TTCTTGGAAT TGCGGGCCGG AAGTGGGTGG TGAGCTTCCA ATGGATTTCG 
GAGTGCTTCA AACAAAAGAA ACTTTTAAAT GAGAGTACCT TTGAAGTGAG AGGTGATGTA 
GTTAATGGAT CCAACCACCA GGGCCCAATG AAAGCTCGAA CAACAGGAGA CAACAATTTG 
CTGATGAAAG ACTTCAATAT CTGTTTTCAG GGGCCTTTTA CAGACATGAC CACAGCTGAA 
ATGGAGTTGA TGGTTGAGCT GTGTGGAGCG ACTGTTGTCA AAGATCCACT TCTGCTCCAC 
AGTAAACCGA CTCATCATCA GCTGATCATC GTTCAGTCCG GATCAGAATC CTACAAGAGC 
CTCTCTAGAA AGGCTACAGT TGTGACTCGG GGGTGGCTGT TGGACACCGT GGCAACCTAC 
ACCATCCAAG ACTTCAGGAC CTACAGAGCT GACCTGAGAG CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca1 ( XM_011602400.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011600702.1 Takifugu rubripes breast cancer 1, early onset (brca1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo21406
Clone ID Related Accession (Same CDS sequence)	XM_011602400.1
Accession Version	XM_011602400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	breast cancer type 1 susceptibility protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAATA AAGGAGAAGA AGATTTTAGT TGCGGGAAAA CTGAAGCTTG TCATTTCTGG 
AATTGTACGC TTCATCTACG AGAAAATAAG GACATTAAAA TAATCACCAT GGAAGCAGCA 
AAAGCCGCAG ATGTTAAGAA AGGGATTTCA GTGCTTTGGG AGAGTCTACA GTGTCCAATA 
TGCTTGGATA TATTGACTGC ACCTGTATCT ACCAAGTGTG ACCATCAATT TTGCAAATTC 
TGTATCTCGA AACTTTTAAG CAACACCAAA CAAAACAAGG CTAACTGTCC AGTATGCAAA 
TCCAAGATAA CCAAAAGGAG CTTACAAGAG AGCCCTGGGT TTCAAAGACT TGTCACAGGA 
TTACAGGGAA TGATTCTGGC ATATGAAAAT GACACAGGCA CAAACTATTT TACTGGGCTG 
TCCAGGCAAA CACAGCAACC ACATGTTGCA GATATCAAAA ATGCTCAACA TCATAAGGAA 
CAATCAGATG AAAATGCTTC CTGTACGGAA GATGATAATG AAGATCTTCC AAAGTCTTAT 
TCTTCAACTA GAGCAGCCCA GGATGGATTT GCAAGGCTTA TGGGCCTAGA AGACACAAGT 
CCTCTGACTA CAGAAATCGA TGGCCTGGAC AGTGGTCTTG GAGAGGCCCC GCCAACTTGT 
GACAAAAAAA TGTACAGCCC AAGAAAGTTG GAGAAGGTTT CCTTTCAGCC AGCATTAATA 
CCAGATAAGG ATGAACACCC ACATTTACAA ACACCTTCAA AGAAGAAGCA TAAAAAGGTT 
TTAGAGCCTG ATAAGATTCT CGATCAGAAA CAGAAGAAAA GTCTAGAGAA AGTTGCAGAG 
TGGCTCATGA AAGTTCCATC CGAGCAAAGC CTGGAATTGG AGAACCTGGA TGAAGATGGC 
GATGACTCCG ACAGCTGCTC ATCCACCTCA ACAATAGATC TTGGGCTACT CCGCTGTGAA 
TCAAATCCTA TGAGAGCTCG TGGAAAAGCC CTGGAAGATC AGGTGTTTGG GGCGATTTAT 
AAAAGACAGC GAAGAGGGAA GGGAATCGTA AAACCGATAG AAGCGGCACT CGAAATGGTT 
TGTTTAAATC ATTCTGACAA AAATACCTCT GAAGATAAAA GCAGAGAAGA TAAGGAAGAG 
GTGCACTCGA TAAGAGAGCA AGAACAAAAC ACCAGTAGCA ACATATTAAA AGAAGAGATT 
GCAGTCTTGG AGGATTGTGG AGGAATCGTT GAGCCAACTC ACATGTCAGA AAATGATCAA 
AACAACAAAG ATGAAGTACC TCATCTAGTT TCTGATATTG GACAACAGCA ACCAGAAACC 
ATTGCAAAGA GAACACGGTC TTCTCTGCAG CAAGTCGACT GTGATTTGTT GAAGTGCACA 
CAAAAAAAGC CAGAAGATAG TGGGAAAAAG AAAATTGCCC AGAGAGGAAG TAAAGGCATC 
AAGTCTGAAA AATCAAAGTC TGCCAGAATG TCAAAACCGC TTGTTTTGGT TACGGTTGAA 
AAGCTAGACA CTAGTCCCAA GATTAGACCG CAGTCAGAAA AAGTTGAAGT TCACATTGAA 
AACTATCCCA GCAGTGGTGA CCAAGAAGTT CCTTTAGGTA GATGTACCAG GAAAAGTAGA 
AGACTTCAGG TCTTCACTAA GGAAGATGAC AAGAAAGAAA CATCAAGATT CAATGTCCCA 
GAAAAACAAC ACAATTCAAA ATACCCCAAC TTTGAATGTG AAATACTGGA TAATATCAAA 
TCACAAAATT GTAAATACCA AACACAACAA GCAAACAGAA ATGGATGTAT TTATAATCAA 
GGTATCCAAG AAATAGAAAA CATGGATTCT GGTGAGAGAG CATCTTCTTT GAGATCAGAA 
GAAGATACTA AAACTTCTCT TTCTGAAGTC CAAAATACCG AAATTTTGTC TGAAACTCCT 
CGCTGCGTCG CTGTGTTCCC AAGTTGTGCT GAACTAGCTC CGGCAGAACC AACTAATCAA 
CCCCCAAATA CTGTCCAGTT GGTGACTGAA CTCGCTGAAA AGGAACGCGA GCAACAGAAC 
GATAGTGAGC AGGACACAGA ACAGTTGGTC AGGAGCTTTA AAGCTACTAA AAGAAAATCT 
TTCCATCTCG GTACTGGTCC AAATATGAAA AGGAGCCATT TATTAGTTCA TGAAAACACC 
CAAAGTCCAG GAACTGAAGA GAACGAAAAT TATTATGAAT CTTTGCCAAA ACAAGTGCAG 
CCAGTGATGG CTAAAATAAC CAACAGTCAG GTTTTAATTG ACAGTCAAAA CATGTCTGGT 
AGTGACTTGA TCTCACCGTC CTGCTCACCC AGCCTGACGA GAAAAGCCTT GGGCCTTCAA 
AGCAGATGTT CAGTGGAAGT TGGAAATTGT GTCCTCGGTA ATAGTGCCAG TAGCCCCCTT 
TCTCCTAATA ACGAATCGAA GCATGTAGTA CAAAGTTCCT CTCCTCTCAT ACTAACAAAG 
GGAAACTCGA CAATCTGCTT TGCAACTGAA AAACCTTCTC AAATTTCAGA GAGCAAAACT 
GATTTTATAA TGATGAAGGA GACACGAGGT AATACTTCAC TGCACAGTTT TAATGGCAGC 
ACTGCTGAGG AACTTTTAGA TGCACAGTCG TCATTAACCC CGGATGGCCT GGGAATGCCA 
GTCCCAGAGG TGACATACAG CCAAGGGAGC AGAGAGCTCA GCAGGTGCAG GAAAAGGACA 
AAGTCTCAGA AGCTGGACTC TTCATCAGAT TGTACAGAAG AGGAATTTCC ATGTTTTTCA 
AAAATCCTCA ACAAAACTGC CCCCCCTGGT GCTAATCCTC CAGCAAGTCC CAGCCTTGAT 
TGTGTTAATT CCAGCCAAGC ATCTGTGGAC TTGTTTGACA TGCCAAATGA GTGTGCCATA 
AATAATGTCG AGTCATCGCA ATTTTCAAGC GAGGTCCTCG TTACGCAGCA AAAAATAGAG 
ATGAAGAAGG AGCTGGTGAG GTTGGAAAAG TTGATGGCAC TGGTGACAGA GGTGCTACAC 
GAGAAGGAAA CCAGCCCAGC AAAAAACATC CAGAATAAAA CCAACCTGAG CAGCAAAATA 
ACAGGCTCAG ATGCTGACAA ACTGCGCCCG AATGTCCAGG ATGAAGATTT TAACCAAGAA 
ACATTCCCAG AAGAAGCACT AGAACCCAAC ACAAGATATT CTGATGCGCC TCCTTCTGGT 
TCCAAGCACA GCAGGACGAC AGAGATGAAC TCCAGAACAT CCAACACAGT TGGGTTAAGT 
TCAGCTGCAA AGACTGATGG TTCCCCATCA GCCGGCCACG AAGACAAAGA AAATAACAAG 
CCTGAGGGAG ACAGAAGCCA AGCCAAGATG CTCCTGGTGA CGTCTGGACT GGGCGCCAGT 
GAACAGATAA CAGTGAAAAA GTTCGCTAAG CGGATTGGCG CCGTTGTGGT CTCCCAGGTG 
ACACCAGAGG TGACTCACAT TGTCATGCAC ACAGATGAAC AACTGGTGTG TGAGCGCACA 
CTGAAGTACT TTCTTGGAAT TGCGGGCCGG AAGTGGGTGG TGAGCTTCCA ATGGATTTCG 
GAGTGCTTCA AACAAAAGAA ACTTTTAAAT GAGAGTACCT TTGAAGTGAG AGGTGATGTA 
GTTAATGGAT CCAACCACCA GGGCCCAATG AAAGCTCGAA CAACAGGAGA CAACAATTTG 
CTGATGAAAG ACTTCAATAT CTGTTTTCAG GGGCCTTTTA CAGACATGAC CACAGCTGAA 
ATGGAGTTGA TGGTTGAGCT GTGTGGAGCG ACTGTTGTCA AAGATCCACT TCTGCTCCAC 
AGTAAACCGC CTCTGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011600702.1
CDS	56..3856
Translation	MLNKGEEDFSCGKTEACHFWNCTLHLRENKDIKIITMEAAKAADVKKGISVLWESLQCPICLDILTAPVSTKCDHQFCKFCISKLLSNTKQNKANCPVCKSKITKRSLQESPGFQRLVTGLQGMILAYENDTGTNYFTGLSRQTQQPHVADIKNAQHHKEQSDENASCTEDDNEDLPKSYSSTRAAQDGFARLMGLEDTSPLTTEIDGLDSGLGEAPPTCDKKMYSPRKLEKVSFQPALIPDKDEHPHLQTPSKKKHKKVLEPDKILDQKQKKSLEKVAEWLMKVPSEQSLELENLDEDGDDSDSCSSTSTIDLGLLRCESNPMRARGKALEDQVFGAIYKRQRRGKGIVKPIEAALEMVCLNHSDKNTSEDKSREDKEEVHSIREQEQNTSSNILKEEIAVLEDCGGIVEPTHMSENDQNNKDEVPHLVSDIGQQQPETIAKRTRSSLQQVDCDLLKCTQKKPEDSGKKKIAQRGSKGIKSEKSKSARMSKPLVLVTVEKLDTSPKIRPQSEKVEVHIENYPSSGDQEVPLGRCTRKSRRLQVFTKEDDKKETSRFNVPEKQHNSKYPNFECEILDNIKSQNCKYQTQQANRNGCIYNQGIQEIENMDSGERASSLRSEEDTKTSLSEVQNTEILSETPRCVAVFPSCAELAPAEPTNQPPNTVQLVTELAEKEREQQNDSEQDTEQLVRSFKATKRKSFHLGTGPNMKRSHLLVHENTQSPGTEENENYYESLPKQVQPVMAKITNSQVLIDSQNMSGSDLISPSCSPSLTRKALGLQSRCSVEVGNCVLGNSASSPLSPNNESKHVVQSSSPLILTKGNSTICFATEKPSQISESKTDFIMMKETRGNTSLHSFNGSTAEELLDAQSSLTPDGLGMPVPEVTYSQGSRELSRCRKRTKSQKLDSSSDCTEEEFPCFSKILNKTAPPGANPPASPSLDCVNSSQASVDLFDMPNECAINNVESSQFSSEVLVTQQKIEMKKELVRLEKLMALVTEVLHEKETSPAKNIQNKTNLSSKITGSDADKLRPNVQDEDFNQETFPEEALEPNTRYSDAPPSGSKHSRTTEMNSRTSNTVGLSSAAKTDGSPSAGHEDKENNKPEGDRSQAKMLLVTSGLGASEQITVKKFAKRIGAVVVSQVTPEVTHIVMHTDEQLVCERTLKYFLGIAGRKWVVSFQWISECFKQKKLLNESTFEVRGDVVNGSNHQGPMKARTTGDNNLLMKDFNICFQGPFTDMTTAEMELMVELCGATVVKDPLLLHSKPPLR

Target ORF information:

RefSeq Version	XM_011602400.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes breast cancer 1, early onset (brca1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602400.1

ORF Insert Sequence:

ATGTTGAATA AAGGAGAAGA AGATTTTAGT TGCGGGAAAA CTGAAGCTTG TCATTTCTGG 
AATTGTACGC TTCATCTACG AGAAAATAAG GACATTAAAA TAATCACCAT GGAAGCAGCA 
AAAGCCGCAG ATGTTAAGAA AGGGATTTCA GTGCTTTGGG AGAGTCTACA GTGTCCAATA 
TGCTTGGATA TATTGACTGC ACCTGTATCT ACCAAGTGTG ACCATCAATT TTGCAAATTC 
TGTATCTCGA AACTTTTAAG CAACACCAAA CAAAACAAGG CTAACTGTCC AGTATGCAAA 
TCCAAGATAA CCAAAAGGAG CTTACAAGAG AGCCCTGGGT TTCAAAGACT TGTCACAGGA 
TTACAGGGAA TGATTCTGGC ATATGAAAAT GACACAGGCA CAAACTATTT TACTGGGCTG 
TCCAGGCAAA CACAGCAACC ACATGTTGCA GATATCAAAA ATGCTCAACA TCATAAGGAA 
CAATCAGATG AAAATGCTTC CTGTACGGAA GATGATAATG AAGATCTTCC AAAGTCTTAT 
TCTTCAACTA GAGCAGCCCA GGATGGATTT GCAAGGCTTA TGGGCCTAGA AGACACAAGT 
CCTCTGACTA CAGAAATCGA TGGCCTGGAC AGTGGTCTTG GAGAGGCCCC GCCAACTTGT 
GACAAAAAAA TGTACAGCCC AAGAAAGTTG GAGAAGGTTT CCTTTCAGCC AGCATTAATA 
CCAGATAAGG ATGAACACCC ACATTTACAA ACACCTTCAA AGAAGAAGCA TAAAAAGGTT 
TTAGAGCCTG ATAAGATTCT CGATCAGAAA CAGAAGAAAA GTCTAGAGAA AGTTGCAGAG 
TGGCTCATGA AAGTTCCATC CGAGCAAAGC CTGGAATTGG AGAACCTGGA TGAAGATGGC 
GATGACTCCG ACAGCTGCTC ATCCACCTCA ACAATAGATC TTGGGCTACT CCGCTGTGAA 
TCAAATCCTA TGAGAGCTCG TGGAAAAGCC CTGGAAGATC AGGTGTTTGG GGCGATTTAT 
AAAAGACAGC GAAGAGGGAA GGGAATCGTA AAACCGATAG AAGCGGCACT CGAAATGGTT 
TGTTTAAATC ATTCTGACAA AAATACCTCT GAAGATAAAA GCAGAGAAGA TAAGGAAGAG 
GTGCACTCGA TAAGAGAGCA AGAACAAAAC ACCAGTAGCA ACATATTAAA AGAAGAGATT 
GCAGTCTTGG AGGATTGTGG AGGAATCGTT GAGCCAACTC ACATGTCAGA AAATGATCAA 
AACAACAAAG ATGAAGTACC TCATCTAGTT TCTGATATTG GACAACAGCA ACCAGAAACC 
ATTGCAAAGA GAACACGGTC TTCTCTGCAG CAAGTCGACT GTGATTTGTT GAAGTGCACA 
CAAAAAAAGC CAGAAGATAG TGGGAAAAAG AAAATTGCCC AGAGAGGAAG TAAAGGCATC 
AAGTCTGAAA AATCAAAGTC TGCCAGAATG TCAAAACCGC TTGTTTTGGT TACGGTTGAA 
AAGCTAGACA CTAGTCCCAA GATTAGACCG CAGTCAGAAA AAGTTGAAGT TCACATTGAA 
AACTATCCCA GCAGTGGTGA CCAAGAAGTT CCTTTAGGTA GATGTACCAG GAAAAGTAGA 
AGACTTCAGG TCTTCACTAA GGAAGATGAC AAGAAAGAAA CATCAAGATT CAATGTCCCA 
GAAAAACAAC ACAATTCAAA ATACCCCAAC TTTGAATGTG AAATACTGGA TAATATCAAA 
TCACAAAATT GTAAATACCA AACACAACAA GCAAACAGAA ATGGATGTAT TTATAATCAA 
GGTATCCAAG AAATAGAAAA CATGGATTCT GGTGAGAGAG CATCTTCTTT GAGATCAGAA 
GAAGATACTA AAACTTCTCT TTCTGAAGTC CAAAATACCG AAATTTTGTC TGAAACTCCT 
CGCTGCGTCG CTGTGTTCCC AAGTTGTGCT GAACTAGCTC CGGCAGAACC AACTAATCAA 
CCCCCAAATA CTGTCCAGTT GGTGACTGAA CTCGCTGAAA AGGAACGCGA GCAACAGAAC 
GATAGTGAGC AGGACACAGA ACAGTTGGTC AGGAGCTTTA AAGCTACTAA AAGAAAATCT 
TTCCATCTCG GTACTGGTCC AAATATGAAA AGGAGCCATT TATTAGTTCA TGAAAACACC 
CAAAGTCCAG GAACTGAAGA GAACGAAAAT TATTATGAAT CTTTGCCAAA ACAAGTGCAG 
CCAGTGATGG CTAAAATAAC CAACAGTCAG GTTTTAATTG ACAGTCAAAA CATGTCTGGT 
AGTGACTTGA TCTCACCGTC CTGCTCACCC AGCCTGACGA GAAAAGCCTT GGGCCTTCAA 
AGCAGATGTT CAGTGGAAGT TGGAAATTGT GTCCTCGGTA ATAGTGCCAG TAGCCCCCTT 
TCTCCTAATA ACGAATCGAA GCATGTAGTA CAAAGTTCCT CTCCTCTCAT ACTAACAAAG 
GGAAACTCGA CAATCTGCTT TGCAACTGAA AAACCTTCTC AAATTTCAGA GAGCAAAACT 
GATTTTATAA TGATGAAGGA GACACGAGGT AATACTTCAC TGCACAGTTT TAATGGCAGC 
ACTGCTGAGG AACTTTTAGA TGCACAGTCG TCATTAACCC CGGATGGCCT GGGAATGCCA 
GTCCCAGAGG TGACATACAG CCAAGGGAGC AGAGAGCTCA GCAGGTGCAG GAAAAGGACA 
AAGTCTCAGA AGCTGGACTC TTCATCAGAT TGTACAGAAG AGGAATTTCC ATGTTTTTCA 
AAAATCCTCA ACAAAACTGC CCCCCCTGGT GCTAATCCTC CAGCAAGTCC CAGCCTTGAT 
TGTGTTAATT CCAGCCAAGC ATCTGTGGAC TTGTTTGACA TGCCAAATGA GTGTGCCATA 
AATAATGTCG AGTCATCGCA ATTTTCAAGC GAGGTCCTCG TTACGCAGCA AAAAATAGAG 
ATGAAGAAGG AGCTGGTGAG GTTGGAAAAG TTGATGGCAC TGGTGACAGA GGTGCTACAC 
GAGAAGGAAA CCAGCCCAGC AAAAAACATC CAGAATAAAA CCAACCTGAG CAGCAAAATA 
ACAGGCTCAG ATGCTGACAA ACTGCGCCCG AATGTCCAGG ATGAAGATTT TAACCAAGAA 
ACATTCCCAG AAGAAGCACT AGAACCCAAC ACAAGATATT CTGATGCGCC TCCTTCTGGT 
TCCAAGCACA GCAGGACGAC AGAGATGAAC TCCAGAACAT CCAACACAGT TGGGTTAAGT 
TCAGCTGCAA AGACTGATGG TTCCCCATCA GCCGGCCACG AAGACAAAGA AAATAACAAG 
CCTGAGGGAG ACAGAAGCCA AGCCAAGATG CTCCTGGTGA CGTCTGGACT GGGCGCCAGT 
GAACAGATAA CAGTGAAAAA GTTCGCTAAG CGGATTGGCG CCGTTGTGGT CTCCCAGGTG 
ACACCAGAGG TGACTCACAT TGTCATGCAC ACAGATGAAC AACTGGTGTG TGAGCGCACA 
CTGAAGTACT TTCTTGGAAT TGCGGGCCGG AAGTGGGTGG TGAGCTTCCA ATGGATTTCG 
GAGTGCTTCA AACAAAAGAA ACTTTTAAAT GAGAGTACCT TTGAAGTGAG AGGTGATGTA 
GTTAATGGAT CCAACCACCA GGGCCCAATG AAAGCTCGAA CAACAGGAGA CAACAATTTG 
CTGATGAAAG ACTTCAATAT CTGTTTTCAG GGGCCTTTTA CAGACATGAC CACAGCTGAA 
ATGGAGTTGA TGGTTGAGCT GTGTGGAGCG ACTGTTGTCA AAGATCCACT TCTGCTCCAC 
AGTAAACCGC CTCTGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca1 ( XM_003961258.3 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003961307.3 Takifugu rubripes BRCA1 DNA repair associated (brca1), mRNA.

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CloneID	OTo42595
Clone ID Related Accession (Same CDS sequence)	XM_003961258.3
Accession Version	XM_003961258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	breast cancer type 1 susceptibility protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003961258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGTG TATCGTGTTG TTACCTCTCC ACAACGTTTG GTGGCGCTAA TCGCGGTGGA 
TACAGAGCGA TGTTGAATAA AGGAGAAGAA GATTTTAGTT GCGGGAAAAC TGAAGCTTGT 
CATTTCTGGA ATTGTACGCT TCATCTACGA GAAAATAAGG ACATTAAAAT AATCACCATG 
GAAGCAGCAA AAGCCGCAGA TGTTAAGAAA GGGATTTCAG TGCTTTGGGA GAGTCTACAG 
TGTCCAATAT GCTTGGATAT ATTGACTGCA CCTGTATCTA CCAAGTGTGA CCATCAATTT 
TGCAAATTCT GTATCTCGAA ACTTTTAAGC AACACCAAAC AAAACAAGGC TAACTGTCCA 
GTATGCAAAT CCAAGATAAC CAAAAGGAGC TTACAAGAGA GCCCTGGGTT TCAAAGACTT 
GTCACAGGAT TACAGGGAAT GATTCTGGCA TATGAAAATG ACACAGGCAC AAACTATTTT 
ACTGGGCTGT CCAGGCAAAC ACAGCAACCA CATGTTGCAG ATATCAAAAA TGCTCAACAT 
CATAAGGAAC AATCAGATGA AAATGCTTCC TGTACGGAAG ATGATAATGA AGATCTTCCA 
AAGTCTTATT CTTCAACTAG AGCAGCCCAG GATGGATTTG CAAGGCTTAT GGGCCTAGAA 
GACACAAGTC CTCTGACTAC AGAAATCGAT GGCCTGGACA GTGGTCTTGG AGAGGCCCCG 
CCAACTTGTG ACAAAAAAAT GTACAGCCCA AGAAAGTTGG AGAAGGTTTC CTTTCAGCCA 
GCATTAATAC CAGATAAGGA TGAACACCCA CATTTACAAA CACCTTCAAA GAAGAAGCAT 
AAAAAGGTTT TAGAGCCTGA TAAGATTCTC GATCAGAAAC AGAAGAAAAG TCTAGAGAAA 
GTTGCAGAGT GGCTCATGAA AGTTCCATCC GAGCAAAGCC TGGAATTGGA GAACCTGGAT 
GAAGATGGCG ATGACTCCGA CAGCTGCTCA TCCACCTCAA CAATAGATCT TGGGCTACTC 
CGCTGTGAAT CAAATCCTAT GAGAGCTCGT GGAAAAGCCC TGGAAGATCA GGTGTTTGGG 
GCGATTTATA AAAGACAGCG AAGAGGGAAG GGAATCGTAA AACCGATAGA AGCGGCACTC 
GAAATGGTTT GTTTAAATCA TTCTGACAAA AATACCTCTG AAGATAAAAG CAGAGAAGAT 
AAGGAAGAGG TGCACTCGAT AAGAGAGCAA GAACAAAACA CCAGTAGCAA CATATTAAAA 
GAAGAGATTG CAGTCTTGGA GGATTGTGGA GGAATCGTTG AGCCAACTCA CATGTCAGAA 
AATGATCAAA ACAACAAAGA TGAAGTACCT CATCTAGTTT CTGATATTGG ACAACAGCAA 
CCAGAAACCA TTGCAAAGAG AACACGGTCT TCTCTGCAGC AAGTCGACTG TGATTTGTTG 
AAGTGCACAC AAAAAAAGCC AGAAGATAGT GGGAAAAAGA AAATTGCCCA GAGAGGAAGT 
AAAGGCATCA AGTCTGAAAA ATCAAAGTCT GCCAGAATGT CAAAACCGCT TGTTTTGGTT 
ACGGTTGAAA AGCTAGACAC TAGTCCCAAG ATTAGACCGC AGTCAGAAAA AGTTGAAGTT 
CACATTGAAA ACTATCCCAG CAGTGGTGAC CAAGAAGTTC CTTTAGGGAG ATGTACCAGG 
AAAAGTAGAA GACTTCAGGT CTTCACTAAG GAAGATGACA AGAAAGAAAC ATCAAGATTC 
AATGTCCCAG AAAAACAACA CAATTCAAAA TACCCCAACT TTGAATGTGA AATACTGGAT 
AATATCAAAT CACAAAATTG TAAATACCAA ACACAACAAG CAAACAGAAA TGGATGTATT 
TATAATCAAG GTATCCAAGA AATAGAAAAC ATGGATTCTG GTGAGAGAGC ATCTTCTTTG 
AGATCAGAAG AAGATACTAA AACTTCTCTT TCTGAAGTCC AAAATACCGA AATTTTGTCT 
GAAACTCCTC GCTGCGTCGC TGTGTTCCCA AGTTGTGCTG AACTAGCTCC GGCAGAACCA 
ACTAATCAAC CCCCAAATAC TGTCCAGTTG GTGACTGAAC TCGCTGAAAA GGAACGCGAG 
CAACAGAACG ATAGTGAGCA GGACACAGAA CAGTTGGTCA GGAGCTTTAA AGCTACTAAA 
AGAAAATCTT TCCATCTCGG TACTGGTCCA AATATGAAAA GGAGCCATTT ATTAGTTCAT 
GAAAACACCC AAAGTCCAGG AACTGAAGAG AACGAAAATT ATTATGAATC TTTGCCAAAA 
CAAGTGCAGC CAGTGATGGC TAAAATAACC AACAGTCAGG TTTTAATTGA CAGTCAAAAC 
ATGTCTGGTA GTGACTTGAT CTCACCGTCC TGCTCACCCA GCCTGACGAG AAAAGCCTTG 
GGCCTTCAAA GCAGATGTTC AGTGGAAGTT GGAAATTGTG TCCTCGGTAA TAGTGCCAGT 
AGCCCCCTTT CTCCTAATAA CGAATCGAAG CATGTAGTAC AAAGTTCCTC TCCTCTCATA 
CTAACAAAGG GAAACTCGAC AATCTGCTTT GCAACTGAAA AACCTTCTCA AATTTCAGAG 
AGCAAAACTG ATTTTATAAT GATGAAGGAG ACACGAGGTA ATACTTCACT GCACAGTTTT 
AATGGCAGCA CTGCTGAGGA ACTTTTAGAT GCACAGTCGT CATTAACCCC GGATGGCCTG 
GGAATGCCAG TCCCAGAGGT GACATACAGC CAAGGGAGCA GAGAGCTCAG CAGGTGCAGG 
AAAAGGACAA AGTCTCAGAA GCTGGACTCT TCATCAGATT GTACAGAAGA GGAATTTCCA 
TGTTTTTCAA AAATCCTCAA CAAAACTGCC CCCCCTGGTG CTAATCCTCC AGCAAGTCCC 
AGCCTTGATT GTGTTAATTC CAGCCAAGCA TCTGTGGACT TGTTTGACAT GCCAAATGAG 
TGTGCCATAA ATAATGTCGA GTCATCGCAA TTTTCAAGCG AGGTCCTTGT TACGCAGCAA 
AAAATAGAGA TGAAGAAGGA GCTGGTGAGG TTGGAAAAGT TGATGGCACT GGTGACAGAG 
GTGCTACACG AGAAGGAAAC CAGCCCAGCA AAAAACATCA AGAATAAAAC CAACCTGAGC 
AGCAAAATAA CAGGCTCAGA TGCTGACAAA CTGCGCCCGA ATGTCCAGGA TGAAGATTTT 
AACCAAGAAA CATTCCCAGA AGAAGCACTA GAACCCAACA CAAGATATTC TGATGCGCCT 
CCTTCTGGTT CCAAGCACAG CAGGACGACA GAGATGAACT CCAGAACATC CAACACAGTT 
GGGTTAAGTT CAGCTGCAAA GACTGATGGT TCCCCATCAG CCGGCCACGA AGACAAAGAA 
AATAACAAGC CTGAGGGAGA CAGAAGCCAA GCCAAGATGC TCCTGGTGAC GTCTGGACTG 
GGCGCCAGTG AACAGATAAC AGTGAAAAAG TTCGCTAAGC GGATTGGCGC CGTTGTGGTC 
TCCCAGGTGA CACCAGAGGT GACTCACATT GTCATGCACA CAGATGAACA ACTGGTGTGT 
GAGCGCACAC TGAAGTACTT TCTTGGAATT GCGGGCCGGA AGTGGGTGGT GAGCTTCCAA 
TGGATTTCGG AGTGCTTCAA ACAAAAGAAA CTTTTAAATG AGAGTACCTT TGAAGTGAGA 
GGTGATGTAG TTAATGGATC CAACCACCAG GGCCCAATGA AAGCTCGAAC AACAGGAGAC 
AACAATTTGC TGATGAAAGA CTTCAATATC TGTTTTCAGG GGCCTTTTAC AGACATGACC 
ACAGCTGAAA TGGAGTTGAT GGTTGAGCTG TGTGGAGCGA CTGTTGTCAA AGATCCACTT 
CTGCTCCACA GTAAACCGAC TCATCATCAG CTGATCATCG TTCAGTCCGG ATCAGAATCC 
TACAAGAGCC TCTCTAGAAA GGCTACAGTT GTGACTCGGG GGTGGCTGTT GGACACCGTG 
GCAACCTACA CCATCCAAGA CTTCAGGACC TACAGAGCTG ACCTGAGAGC CGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961307.3
CDS	16..4032
Translation	MRCVSCCYLSTTFGGANRGGYRAMLNKGEEDFSCGKTEACHFWNCTLHLRENKDIKIITMEAAKAADVKKGISVLWESLQCPICLDILTAPVSTKCDHQFCKFCISKLLSNTKQNKANCPVCKSKITKRSLQESPGFQRLVTGLQGMILAYENDTGTNYFTGLSRQTQQPHVADIKNAQHHKEQSDENASCTEDDNEDLPKSYSSTRAAQDGFARLMGLEDTSPLTTEIDGLDSGLGEAPPTCDKKMYSPRKLEKVSFQPALIPDKDEHPHLQTPSKKKHKKVLEPDKILDQKQKKSLEKVAEWLMKVPSEQSLELENLDEDGDDSDSCSSTSTIDLGLLRCESNPMRARGKALEDQVFGAIYKRQRRGKGIVKPIEAALEMVCLNHSDKNTSEDKSREDKEEVHSIREQEQNTSSNILKEEIAVLEDCGGIVEPTHMSENDQNNKDEVPHLVSDIGQQQPETIAKRTRSSLQQVDCDLLKCTQKKPEDSGKKKIAQRGSKGIKSEKSKSARMSKPLVLVTVEKLDTSPKIRPQSEKVEVHIENYPSSGDQEVPLGRCTRKSRRLQVFTKEDDKKETSRFNVPEKQHNSKYPNFECEILDNIKSQNCKYQTQQANRNGCIYNQGIQEIENMDSGERASSLRSEEDTKTSLSEVQNTEILSETPRCVAVFPSCAELAPAEPTNQPPNTVQLVTELAEKEREQQNDSEQDTEQLVRSFKATKRKSFHLGTGPNMKRSHLLVHENTQSPGTEENENYYESLPKQVQPVMAKITNSQVLIDSQNMSGSDLISPSCSPSLTRKALGLQSRCSVEVGNCVLGNSASSPLSPNNESKHVVQSSSPLILTKGNSTICFATEKPSQISESKTDFIMMKETRGNTSLHSFNGSTAEELLDAQSSLTPDGLGMPVPEVTYSQGSRELSRCRKRTKSQKLDSSSDCTEEEFPCFSKILNKTAPPGANPPASPSLDCVNSSQASVDLFDMPNECAINNVESSQFSSEVLVTQQKIEMKKELVRLEKLMALVTEVLHEKETSPAKNIKNKTNLSSKITGSDADKLRPNVQDEDFNQETFPEEALEPNTRYSDAPPSGSKHSRTTEMNSRTSNTVGLSSAAKTDGSPSAGHEDKENNKPEGDRSQAKMLLVTSGLGASEQITVKKFAKRIGAVVVSQVTPEVTHIVMHTDEQLVCERTLKYFLGIAGRKWVVSFQWISECFKQKKLLNESTFEVRGDVVNGSNHQGPMKARTTGDNNLLMKDFNICFQGPFTDMTTAEMELMVELCGATVVKDPLLLHSKPTHHQLIIVQSGSESYKSLSRKATVVTRGWLLDTVATYTIQDFRTYRADLRAA

Target ORF information:

RefSeq Version	XM_003961258.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes BRCA1 DNA repair associated (brca1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961258.3

ORF Insert Sequence:

ATGCGTTGTG TATCGTGTTG TTACCTCTCC ACAACGTTTG GTGGCGCTAA TCGCGGTGGA 
TACAGAGCGA TGTTGAATAA AGGAGAAGAA GATTTTAGTT GCGGGAAAAC TGAAGCTTGT 
CATTTCTGGA ATTGTACGCT TCATCTACGA GAAAATAAGG ACATTAAAAT AATCACCATG 
GAAGCAGCAA AAGCCGCAGA TGTTAAGAAA GGGATTTCAG TGCTTTGGGA GAGTCTACAG 
TGTCCAATAT GCTTGGATAT ATTGACTGCA CCTGTATCTA CCAAGTGTGA CCATCAATTT 
TGCAAATTCT GTATCTCGAA ACTTTTAAGC AACACCAAAC AAAACAAGGC TAACTGTCCA 
GTATGCAAAT CCAAGATAAC CAAAAGGAGC TTACAAGAGA GCCCTGGGTT TCAAAGACTT 
GTCACAGGAT TACAGGGAAT GATTCTGGCA TATGAAAATG ACACAGGCAC AAACTATTTT 
ACTGGGCTGT CCAGGCAAAC ACAGCAACCA CATGTTGCAG ATATCAAAAA TGCTCAACAT 
CATAAGGAAC AATCAGATGA AAATGCTTCC TGTACGGAAG ATGATAATGA AGATCTTCCA 
AAGTCTTATT CTTCAACTAG AGCAGCCCAG GATGGATTTG CAAGGCTTAT GGGCCTAGAA 
GACACAAGTC CTCTGACTAC AGAAATCGAT GGCCTGGACA GTGGTCTTGG AGAGGCCCCG 
CCAACTTGTG ACAAAAAAAT GTACAGCCCA AGAAAGTTGG AGAAGGTTTC CTTTCAGCCA 
GCATTAATAC CAGATAAGGA TGAACACCCA CATTTACAAA CACCTTCAAA GAAGAAGCAT 
AAAAAGGTTT TAGAGCCTGA TAAGATTCTC GATCAGAAAC AGAAGAAAAG TCTAGAGAAA 
GTTGCAGAGT GGCTCATGAA AGTTCCATCC GAGCAAAGCC TGGAATTGGA GAACCTGGAT 
GAAGATGGCG ATGACTCCGA CAGCTGCTCA TCCACCTCAA CAATAGATCT TGGGCTACTC 
CGCTGTGAAT CAAATCCTAT GAGAGCTCGT GGAAAAGCCC TGGAAGATCA GGTGTTTGGG 
GCGATTTATA AAAGACAGCG AAGAGGGAAG GGAATCGTAA AACCGATAGA AGCGGCACTC 
GAAATGGTTT GTTTAAATCA TTCTGACAAA AATACCTCTG AAGATAAAAG CAGAGAAGAT 
AAGGAAGAGG TGCACTCGAT AAGAGAGCAA GAACAAAACA CCAGTAGCAA CATATTAAAA 
GAAGAGATTG CAGTCTTGGA GGATTGTGGA GGAATCGTTG AGCCAACTCA CATGTCAGAA 
AATGATCAAA ACAACAAAGA TGAAGTACCT CATCTAGTTT CTGATATTGG ACAACAGCAA 
CCAGAAACCA TTGCAAAGAG AACACGGTCT TCTCTGCAGC AAGTCGACTG TGATTTGTTG 
AAGTGCACAC AAAAAAAGCC AGAAGATAGT GGGAAAAAGA AAATTGCCCA GAGAGGAAGT 
AAAGGCATCA AGTCTGAAAA ATCAAAGTCT GCCAGAATGT CAAAACCGCT TGTTTTGGTT 
ACGGTTGAAA AGCTAGACAC TAGTCCCAAG ATTAGACCGC AGTCAGAAAA AGTTGAAGTT 
CACATTGAAA ACTATCCCAG CAGTGGTGAC CAAGAAGTTC CTTTAGGGAG ATGTACCAGG 
AAAAGTAGAA GACTTCAGGT CTTCACTAAG GAAGATGACA AGAAAGAAAC ATCAAGATTC 
AATGTCCCAG AAAAACAACA CAATTCAAAA TACCCCAACT TTGAATGTGA AATACTGGAT 
AATATCAAAT CACAAAATTG TAAATACCAA ACACAACAAG CAAACAGAAA TGGATGTATT 
TATAATCAAG GTATCCAAGA AATAGAAAAC ATGGATTCTG GTGAGAGAGC ATCTTCTTTG 
AGATCAGAAG AAGATACTAA AACTTCTCTT TCTGAAGTCC AAAATACCGA AATTTTGTCT 
GAAACTCCTC GCTGCGTCGC TGTGTTCCCA AGTTGTGCTG AACTAGCTCC GGCAGAACCA 
ACTAATCAAC CCCCAAATAC TGTCCAGTTG GTGACTGAAC TCGCTGAAAA GGAACGCGAG 
CAACAGAACG ATAGTGAGCA GGACACAGAA CAGTTGGTCA GGAGCTTTAA AGCTACTAAA 
AGAAAATCTT TCCATCTCGG TACTGGTCCA AATATGAAAA GGAGCCATTT ATTAGTTCAT 
GAAAACACCC AAAGTCCAGG AACTGAAGAG AACGAAAATT ATTATGAATC TTTGCCAAAA 
CAAGTGCAGC CAGTGATGGC TAAAATAACC AACAGTCAGG TTTTAATTGA CAGTCAAAAC 
ATGTCTGGTA GTGACTTGAT CTCACCGTCC TGCTCACCCA GCCTGACGAG AAAAGCCTTG 
GGCCTTCAAA GCAGATGTTC AGTGGAAGTT GGAAATTGTG TCCTCGGTAA TAGTGCCAGT 
AGCCCCCTTT CTCCTAATAA CGAATCGAAG CATGTAGTAC AAAGTTCCTC TCCTCTCATA 
CTAACAAAGG GAAACTCGAC AATCTGCTTT GCAACTGAAA AACCTTCTCA AATTTCAGAG 
AGCAAAACTG ATTTTATAAT GATGAAGGAG ACACGAGGTA ATACTTCACT GCACAGTTTT 
AATGGCAGCA CTGCTGAGGA ACTTTTAGAT GCACAGTCGT CATTAACCCC GGATGGCCTG 
GGAATGCCAG TCCCAGAGGT GACATACAGC CAAGGGAGCA GAGAGCTCAG CAGGTGCAGG 
AAAAGGACAA AGTCTCAGAA GCTGGACTCT TCATCAGATT GTACAGAAGA GGAATTTCCA 
TGTTTTTCAA AAATCCTCAA CAAAACTGCC CCCCCTGGTG CTAATCCTCC AGCAAGTCCC 
AGCCTTGATT GTGTTAATTC CAGCCAAGCA TCTGTGGACT TGTTTGACAT GCCAAATGAG 
TGTGCCATAA ATAATGTCGA GTCATCGCAA TTTTCAAGCG AGGTCCTTGT TACGCAGCAA 
AAAATAGAGA TGAAGAAGGA GCTGGTGAGG TTGGAAAAGT TGATGGCACT GGTGACAGAG 
GTGCTACACG AGAAGGAAAC CAGCCCAGCA AAAAACATCA AGAATAAAAC CAACCTGAGC 
AGCAAAATAA CAGGCTCAGA TGCTGACAAA CTGCGCCCGA ATGTCCAGGA TGAAGATTTT 
AACCAAGAAA CATTCCCAGA AGAAGCACTA GAACCCAACA CAAGATATTC TGATGCGCCT 
CCTTCTGGTT CCAAGCACAG CAGGACGACA GAGATGAACT CCAGAACATC CAACACAGTT 
GGGTTAAGTT CAGCTGCAAA GACTGATGGT TCCCCATCAG CCGGCCACGA AGACAAAGAA 
AATAACAAGC CTGAGGGAGA CAGAAGCCAA GCCAAGATGC TCCTGGTGAC GTCTGGACTG 
GGCGCCAGTG AACAGATAAC AGTGAAAAAG TTCGCTAAGC GGATTGGCGC CGTTGTGGTC 
TCCCAGGTGA CACCAGAGGT GACTCACATT GTCATGCACA CAGATGAACA ACTGGTGTGT 
GAGCGCACAC TGAAGTACTT TCTTGGAATT GCGGGCCGGA AGTGGGTGGT GAGCTTCCAA 
TGGATTTCGG AGTGCTTCAA ACAAAAGAAA CTTTTAAATG AGAGTACCTT TGAAGTGAGA 
GGTGATGTAG TTAATGGATC CAACCACCAG GGCCCAATGA AAGCTCGAAC AACAGGAGAC 
AACAATTTGC TGATGAAAGA CTTCAATATC TGTTTTCAGG GGCCTTTTAC AGACATGACC 
ACAGCTGAAA TGGAGTTGAT GGTTGAGCTG TGTGGAGCGA CTGTTGTCAA AGATCCACTT 
CTGCTCCACA GTAAACCGAC TCATCATCAG CTGATCATCG TTCAGTCCGG ATCAGAATCC 
TACAAGAGCC TCTCTAGAAA GGCTACAGTT GTGACTCGGG GGTGGCTGTT GGACACCGTG 
GCAACCTACA CCATCCAAGA CTTCAGGACC TACAGAGCTG ACCTGAGAGC CGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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brca1 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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