LOC101062825 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101062825
Entrez Gene ID	101062825
Full Name	toll-like receptor 8
Synonyms	tlr8
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011609158.1	XP_011607460.1	toll-like receptor 8 isoform X2
XM_003961819.2	XP_003961868.2	toll-like receptor 8 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101062825 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101062825 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo00913	XM_011609158.1 Latest version!	Takifugu rubripes toll-like receptor 8 (LOC101062825), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo19905	XM_003961819.2 Latest version!	Takifugu rubripes toll-like receptor 8 (LOC101062825), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101062825 ( XM_011609158.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011607460.1 Takifugu rubripes toll-like receptor 8 (LOC101062825), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo00913
Clone ID Related Accession (Same CDS sequence)	XM_011609158.1
Accession Version	XM_011609158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	toll-like receptor 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGT CGTGTCTGTC GTGGCTTTCT GAGCTCCATC ATGCTGCATG TAAACCTACA 
TGGCTGCTGC CACAGTTCCC CTGCCAAGTC ACAGGATACA ACTCTTCCAG TGTCTTCTTC 
GACTGCTCAA GGCGCCACCT ACATCAAGTC CCCAAAGGTA TAACGAGCAA CGTAACCGAT 
CTCAACCTGT CAGAAAATTC AATCAAGGAG ATTTCGCCTC TTGCATTTTC CACAATGTGG 
AACCTGACCA AGCTCAACCT CAGCTGGGCA AACAAGAAAA CGGGGGTGAA CATCAGCAAT 
AATCTGTTCA GAAATCTGAC AAAGCTGCAG CATCTGCGCC TGAGTGGCAA TGGCATGTAT 
AGAATTCCTG GAAACATCCC TGTTAGCGTG AGGATCCTTA AGATGGGCAA CAACAAGATC 
ACCTCTCTGG ATGCACACAG CCTTGCTGGC TTGAAAAATG TGACTCATTT TTCTTTGTCA 
AGAAACTGCT ACCGTTGGAA TCCTTGTGGG AAACATGTTA GCATTGAGAA GGACAGCTTT 
TCCAGCATGT CCAACTTGGT GTTTCTGGAC TTGTCCTTTA ACAATTTATC TCAGGTCCCG 
AAAGGATTAC CCCAATCATT GCAATTCCTA AAGTTAGATA ACAATAAAAT ACAAAACATA 
TACTCAGATG ATTTTTATGG ATTACAGAAT TTAAAAGAGC TCAAACTAGA AGGCAACTGC 
CCAAGGTGTG AAAATGCTCC ATTTCCATGT GATACTTGTC AGAATATATC TGTCAGGATC 
CATCCTAATG CATTCTATAA TTTGACAAAT TTGGAAACAC TGAACCTGGG AGGAAATTCA 
CTGAAAGAGC TGAATCCCTC TTGGTTTGAG AGACTTTACA AGCTCAAACA ATTGTTCCTT 
GCATTCAACT TTCTGTTAAA ACCAATTACT GAGCAGGGGG CATTCCTAAA ATACATCCCC 
AATATAAAAA AAATGGATCT ATCTTTTAAC TATAACCTAA GGTCCTATCC TAAAACCATC 
AATCTTTCTC CAGAGTTTTC AAATCTGGCA GCCCTTCAAA CATTACATCT GGAGGGCTTG 
GTCTTCCAAA ATATTGGACC GGACACACTT CGACCACTGT ACCAGCTCAA AAATCTGTCT 
GTGCTAAACC TGGGGACTAA CTTCATTATT CAGGCTGATT CTAGCATATT TAAAAAACTA 
TCTCATCTAA AAATGATATA TCTTGGAGAA AACAGGCTGT ATCCCATTCC AGAGAAAAAT 
ATGCCGAGTC TGAGCAGGCA GTACAACCAG AGGTTGGACT TTTCCGTTTC ATCATACATG 
AAACCCCACG GAAGAGATTA TTTCTTCAAG ATATCTCAGA CACTTATCAA GCAAGAGTGC 
TTCGATGCTG GAAAAGTGCT GAGCCTTAAC TCGAATAACT TCCTCTTTAT TTCCACAAAG 
GAGTTTGAAG GAGATATTAA GTGTCTCAAT CTCTCGAGAA ATGGATTCGC ACCAGCGCTC 
AATGGGACAG AGTTTTCCTT CCTGCCTAAT TTGACGTACT TAGATCTGTC ATTCAATAAA 
ATTGATCTGG CCTACAGCTT GGCATTCAAC GACCTAAAGA AGCTACAAGT GCTGGACCTC 
AGTTACAATC CTCACTACTT TAATGTACAA GGGATAACCC ATAAAGTTAA CTTCTTAAGA 
AACCTGCCGG TCCTCAGGGT GCTGAATATG AGCCACAATG ACATTTCCAC ACTGACTACA 
AAATACATGG AAAGCAAGTC TTTAGCGGAG CTTCGCTTCA CACACAATTA TCTTGGGACT 
CTTTGGAAAG AAAATGATCT TTCCTACAAG AAACTTTTTA CTAAACTTAC TAATTTGACC 
ATTTTAGACA TATCCTTCAA CCAAATTATA AAGATCCCTG ATGAGATGTA TAAACATTTG 
CCACAGAACC TCACCACACT GATCATAAGT CACAACTTCC TGACTGATTT TAAATGGAAC 
AAGCTTATAT TTTTACCACA GATTAAAGTT TTAGACCTCA GCTTCAACCG TTTGACCAAC 
GTGACAGGTA TACACATTGC CCACACCCTG ACACTCCTCA ACCTCAAACA TAATGGCATT 
TCTCACTTGG ATGATGGATT TCTAATGGGT GCAAAAAGAC TTCAGGTTCT CAATCTCAAA 
TCCAACCAAC TCACCACCAT TAACGAATCC ACGTTCCAGC CCAGACCTGA GAATCAGTTC 
CAGACTTTGT ACCTGGAGGA AAACCCCTTT CAGTGTACAT GTGATTTACT AGATTTCATC 
TTATGGATCG AAAACAGTGA CGTAAAAATC CCCGGGCTCG CCACGGATGT GAAATGTGAT 
GCACCTGCAA ATCAAAAGGG TCGTGTTCTG ATTAAGTTTG ACATTACTCA GTGTGTAAAC 
AACAGTGAGG CATTCTTGAT CTACATCCTT ACCAGTTCCT TTGTTATTGG TTTTATGCTT 
GTGACCACTG TGGCCCATCT TTTTTACTGG GATGCCACCT ATGTCCTACA CTATATGAAA 
GCTAAGTTAA AGGGATACAG CTCTTTGAAC TCATCAGAGA TTTTGTATGG TGCTTTTGTG 
ACCTACGACA CCAGAGATCC ACATGTCTCT GAATGGGTGA TGAAGAACCT TCTGGTGAAA 
CTAGAGGAGG AAGGAGAGAA GAACCTTCCT CTGTGTCTGG AGGAGAGGGA CTGGACCCCC 
GGAGTACCGC TGGTAGACAA CCTCACTCAG AGCATCCGAT ACAGTCGGAA GACCCTATTT 
GTCCTGACGC AGGATTACGT CAAGACCGGG ATTTTCAAGA TGGCCATGTA TCTGGCACAC 
CAGAGACTGC TTGATGAAAA TGTGGATGTG ATTGTTTTGC TGTTGCTGGA ACCTGTTCTG 
CAGCATTCTC ACTTCCTTCG TCTGCGACGG AGACTATGCG GTGAAAGTGT AGTGGACTGG 
CCGAGAACAG CTGCCGCAGA ACCCTGGTTT TGGCAAAACC TGAGGAATGT GGTCAGGGTG 
GAAAATCAGG TTATGTACAA CAAAAATTAT TCAAAGTACT TCACAAGTAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607460.1
CDS	41..3094
Translation	MLLSCLSWLSELHHAACKPTWLLPQFPCQVTGYNSSSVFFDCSRRHLHQVPKGITSNVTDLNLSENSIKEISPLAFSTMWNLTKLNLSWANKKTGVNISNNLFRNLTKLQHLRLSGNGMYRIPGNIPVSVRILKMGNNKITSLDAHSLAGLKNVTHFSLSRNCYRWNPCGKHVSIEKDSFSSMSNLVFLDLSFNNLSQVPKGLPQSLQFLKLDNNKIQNIYSDDFYGLQNLKELKLEGNCPRCENAPFPCDTCQNISVRIHPNAFYNLTNLETLNLGGNSLKELNPSWFERLYKLKQLFLAFNFLLKPITEQGAFLKYIPNIKKMDLSFNYNLRSYPKTINLSPEFSNLAALQTLHLEGLVFQNIGPDTLRPLYQLKNLSVLNLGTNFIIQADSSIFKKLSHLKMIYLGENRLYPIPEKNMPSLSRQYNQRLDFSVSSYMKPHGRDYFFKISQTLIKQECFDAGKVLSLNSNNFLFISTKEFEGDIKCLNLSRNGFAPALNGTEFSFLPNLTYLDLSFNKIDLAYSLAFNDLKKLQVLDLSYNPHYFNVQGITHKVNFLRNLPVLRVLNMSHNDISTLTTKYMESKSLAELRFTHNYLGTLWKENDLSYKKLFTKLTNLTILDISFNQIIKIPDEMYKHLPQNLTTLIISHNFLTDFKWNKLIFLPQIKVLDLSFNRLTNVTGIHIAHTLTLLNLKHNGISHLDDGFLMGAKRLQVLNLKSNQLTTINESTFQPRPENQFQTLYLEENPFQCTCDLLDFILWIENSDVKIPGLATDVKCDAPANQKGRVLIKFDITQCVNNSEAFLIYILTSSFVIGFMLVTTVAHLFYWDATYVLHYMKAKLKGYSSLNSSEILYGAFVTYDTRDPHVSEWVMKNLLVKLEEEGEKNLPLCLEERDWTPGVPLVDNLTQSIRYSRKTLFVLTQDYVKTGIFKMAMYLAHQRLLDENVDVIVLLLLEPVLQHSHFLRLRRRLCGESVVDWPRTAAAEPWFWQNLRNVVRVENQVMYNKNYSKYFTSK

Target ORF information:

RefSeq Version	XM_011609158.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes toll-like receptor 8 (LOC101062825), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609158.1

ORF Insert Sequence:

ATGCTGCTGT CGTGTCTGTC GTGGCTTTCT GAGCTCCATC ATGCTGCATG TAAACCTACA 
TGGCTGCTGC CACAGTTCCC CTGCCAAGTC ACAGGATACA ACTCTTCCAG TGTCTTCTTC 
GACTGCTCAA GGCGCCACCT ACATCAAGTC CCCAAAGGTA TAACGAGCAA CGTAACCGAT 
CTCAACCTGT CAGAAAATTC AATCAAGGAG ATTTCGCCTC TTGCATTTTC CACAATGTGG 
AACCTGACCA AGCTCAACCT CAGCTGGGCA AACAAGAAAA CGGGGGTGAA CATCAGCAAT 
AATCTGTTCA GAAATCTGAC AAAGCTGCAG CATCTGCGCC TGAGTGGCAA TGGCATGTAT 
AGAATTCCTG GAAACATCCC TGTTAGCGTG AGGATCCTTA AGATGGGCAA CAACAAGATC 
ACCTCTCTGG ATGCACACAG CCTTGCTGGC TTGAAAAATG TGACTCATTT TTCTTTGTCA 
AGAAACTGCT ACCGTTGGAA TCCTTGTGGG AAACATGTTA GCATTGAGAA GGACAGCTTT 
TCCAGCATGT CCAACTTGGT GTTTCTGGAC TTGTCCTTTA ACAATTTATC TCAGGTCCCG 
AAAGGATTAC CCCAATCATT GCAATTCCTA AAGTTAGATA ACAATAAAAT ACAAAACATA 
TACTCAGATG ATTTTTATGG ATTACAGAAT TTAAAAGAGC TCAAACTAGA AGGCAACTGC 
CCAAGGTGTG AAAATGCTCC ATTTCCATGT GATACTTGTC AGAATATATC TGTCAGGATC 
CATCCTAATG CATTCTATAA TTTGACAAAT TTGGAAACAC TGAACCTGGG AGGAAATTCA 
CTGAAAGAGC TGAATCCCTC TTGGTTTGAG AGACTTTACA AGCTCAAACA ATTGTTCCTT 
GCATTCAACT TTCTGTTAAA ACCAATTACT GAGCAGGGGG CATTCCTAAA ATACATCCCC 
AATATAAAAA AAATGGATCT ATCTTTTAAC TATAACCTAA GGTCCTATCC TAAAACCATC 
AATCTTTCTC CAGAGTTTTC AAATCTGGCA GCCCTTCAAA CATTACATCT GGAGGGCTTG 
GTCTTCCAAA ATATTGGACC GGACACACTT CGACCACTGT ACCAGCTCAA AAATCTGTCT 
GTGCTAAACC TGGGGACTAA CTTCATTATT CAGGCTGATT CTAGCATATT TAAAAAACTA 
TCTCATCTAA AAATGATATA TCTTGGAGAA AACAGGCTGT ATCCCATTCC AGAGAAAAAT 
ATGCCGAGTC TGAGCAGGCA GTACAACCAG AGGTTGGACT TTTCCGTTTC ATCATACATG 
AAACCCCACG GAAGAGATTA TTTCTTCAAG ATATCTCAGA CACTTATCAA GCAAGAGTGC 
TTCGATGCTG GAAAAGTGCT GAGCCTTAAC TCGAATAACT TCCTCTTTAT TTCCACAAAG 
GAGTTTGAAG GAGATATTAA GTGTCTCAAT CTCTCGAGAA ATGGATTCGC ACCAGCGCTC 
AATGGGACAG AGTTTTCCTT CCTGCCTAAT TTGACGTACT TAGATCTGTC ATTCAATAAA 
ATTGATCTGG CCTACAGCTT GGCATTCAAC GACCTAAAGA AGCTACAAGT GCTGGACCTC 
AGTTACAATC CTCACTACTT TAATGTACAA GGGATAACCC ATAAAGTTAA CTTCTTAAGA 
AACCTGCCGG TCCTCAGGGT GCTGAATATG AGCCACAATG ACATTTCCAC ACTGACTACA 
AAATACATGG AAAGCAAGTC TTTAGCGGAG CTTCGCTTCA CACACAATTA TCTTGGGACT 
CTTTGGAAAG AAAATGATCT TTCCTACAAG AAACTTTTTA CTAAACTTAC TAATTTGACC 
ATTTTAGACA TATCCTTCAA CCAAATTATA AAGATCCCTG ATGAGATGTA TAAACATTTG 
CCACAGAACC TCACCACACT GATCATAAGT CACAACTTCC TGACTGATTT TAAATGGAAC 
AAGCTTATAT TTTTACCACA GATTAAAGTT TTAGACCTCA GCTTCAACCG TTTGACCAAC 
GTGACAGGTA TACACATTGC CCACACCCTG ACACTCCTCA ACCTCAAACA TAATGGCATT 
TCTCACTTGG ATGATGGATT TCTAATGGGT GCAAAAAGAC TTCAGGTTCT CAATCTCAAA 
TCCAACCAAC TCACCACCAT TAACGAATCC ACGTTCCAGC CCAGACCTGA GAATCAGTTC 
CAGACTTTGT ACCTGGAGGA AAACCCCTTT CAGTGTACAT GTGATTTACT AGATTTCATC 
TTATGGATCG AAAACAGTGA CGTAAAAATC CCCGGGCTCG CCACGGATGT GAAATGTGAT 
GCACCTGCAA ATCAAAAGGG TCGTGTTCTG ATTAAGTTTG ACATTACTCA GTGTGTAAAC 
AACAGTGAGG CATTCTTGAT CTACATCCTT ACCAGTTCCT TTGTTATTGG TTTTATGCTT 
GTGACCACTG TGGCCCATCT TTTTTACTGG GATGCCACCT ATGTCCTACA CTATATGAAA 
GCTAAGTTAA AGGGATACAG CTCTTTGAAC TCATCAGAGA TTTTGTATGG TGCTTTTGTG 
ACCTACGACA CCAGAGATCC ACATGTCTCT GAATGGGTGA TGAAGAACCT TCTGGTGAAA 
CTAGAGGAGG AAGGAGAGAA GAACCTTCCT CTGTGTCTGG AGGAGAGGGA CTGGACCCCC 
GGAGTACCGC TGGTAGACAA CCTCACTCAG AGCATCCGAT ACAGTCGGAA GACCCTATTT 
GTCCTGACGC AGGATTACGT CAAGACCGGG ATTTTCAAGA TGGCCATGTA TCTGGCACAC 
CAGAGACTGC TTGATGAAAA TGTGGATGTG ATTGTTTTGC TGTTGCTGGA ACCTGTTCTG 
CAGCATTCTC ACTTCCTTCG TCTGCGACGG AGACTATGCG GTGAAAGTGT AGTGGACTGG 
CCGAGAACAG CTGCCGCAGA ACCCTGGTTT TGGCAAAACC TGAGGAATGT GGTCAGGGTG 
GAAAATCAGG TTATGTACAA CAAAAATTAT TCAAAGTACT TCACAAGTAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062825 ( XM_003961819.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003961868.2 Takifugu rubripes toll-like receptor 8 (LOC101062825), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo19905
Clone ID Related Accession (Same CDS sequence)	XM_003961819.2
Accession Version	XM_003961819.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	toll-like receptor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003961819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGG CCTGGATGCT GCTGTCGTGT CTGTCGTGGC TTTCTGAGCT CCATCATGCT 
GCATGTAAAC CTACATGGCT GCTGCCACAG TTCCCCTGCC AAGTCACAGG ATACAACTCT 
TCCAGTGTCT TCTTCGACTG CTCAAGGCGC CACCTACATC AAGTCCCCAA AGGTATAACG 
AGCAACGTAA CCGATCTCAA CCTGTCAGAA AATTCAATCA AGGAGATTTC GCCTCTTGCA 
TTTTCCACAA TGTGGAACCT GACCAAGCTC AACCTCAGCT GGGCAAACAA GAAAACGGGG 
GTGAACATCA GCAATAATCT GTTCAGAAAT CTGACAAAGC TGCAGCATCT GCGCCTGAGT 
GGCAATGGCA TGTATAGAAT TCCTGGAAAC ATCCCTGTTA GCGTGAGGAT CCTTAAGATG 
GGCAACAACA AGATCACCTC TCTGGATGCA CACAGCCTTG CTGGCTTGAA AAATGTGACT 
CATTTTTCTT TGTCAAGAAA CTGCTACCGT TGGAATCCTT GTGGGAAACA TGTTAGCATT 
GAGAAGGACA GCTTTTCCAG CATGTCCAAC TTGGTGTTTC TGGACTTGTC CTTTAACAAT 
TTATCTCAGG TCCCGAAAGG ATTACCCCAA TCATTGCAAT TCCTAAAGTT AGATAACAAT 
AAAATACAAA ACATATACTC AGATGATTTT TATGGATTAC AGAATTTAAA AGAGCTCAAA 
CTAGAAGGCA ACTGCCCAAG GTGTGAAAAT GCTCCATTTC CATGTGATAC TTGTCAGAAT 
ATATCTGTCA GGATCCATCC TAATGCATTC TATAATTTGA CAAATTTGGA AACACTGAAC 
CTGGGAGGAA ATTCACTGAA AGAGCTGAAT CCCTCTTGGT TTGAGAGACT TTACAAGCTC 
AAACAATTGT TCCTTGCATT CAACTTTCTG TTAAAACCAA TTACTGAGCA GGGGGCATTC 
CTAAAATACA TCCCCAATAT AAAAAAAATG GATCTATCTT TTAACTATAA CCTAAGGTCC 
TATCCTAAAA CCATCAATCT TTCTCCAGAG TTTTCAAATC TGGCAGCCCT TCAAACATTA 
CATCTGGAGG GCTTGGTCTT CCAAAATATT GGACCGGACA CACTTCGACC ACTGTACCAG 
CTCAAAAATC TGTCTGTGCT AAACCTGGGG ACTAACTTCA TTATTCAGGC TGATTCTAGC 
ATATTTAAAA AACTATCTCA TCTAAAAATG ATATATCTTG GAGAAAACAG GCTGTATCCC 
ATTCCAGAGA AAAATATGCC GAGTCTGAGC AGGCAGTACA ACCAGAGGTT GGACTTTTCC 
GTTTCATCAT ACATGAAACC CCACGGAAGA GATTATTTCT TCAAGATATC TCAGACACTT 
ATCAAGCAAG AGTGCTTCGA TGCTGGAAAA GTGCTGAGCC TTAACTCGAA TAACTTCCTC 
TTTATTTCCA CAAAGGAGTT TGAAGGAGAT ATTAAGTGTC TCAATCTCTC GAGAAATGGA 
TTCGCACCAG CGCTCAATGG GACAGAGTTT TCCTTCCTGC CTAATTTGAC GTACTTAGAT 
CTGTCATTCA ATAAAATTGA TCTGGCCTAC AGCTTGGCAT TCAACGACCT AAAGAAGCTA 
CAAGTGCTGG ACCTCAGTTA CAATCCTCAC TACTTTAATG TACAAGGGAT AACCCATAAA 
GTTAACTTCT TAAGAAACCT GCCGGTCCTC AGGGTGCTGA ATATGAGCCA CAATGACATT 
TCCACACTGA CTACAAAATA CATGGAAAGC AAGTCTTTAG CGGAGCTTCG CTTCACACAC 
AATTATCTTG GGACTCTTTG GAAAGAAAAT GATCTTTCCT ACAAGAAACT TTTTACTAAA 
CTTACTAATT TGACCATTTT AGACATATCC TTCAACCAAA TTATAAAGAT CCCTGATGAG 
ATGTATAAAC ATTTGCCACA GAACCTCACC ACACTGATCA TAAGTCACAA CTTCCTGACT 
GATTTTAAAT GGAACAAGCT TATATTTTTA CCACAGATTA AAGTTTTAGA CCTCAGCTTC 
AACCGTTTGA CCAACGTGAC AGGTATACAC ATTGCCCACA CCCTGACACT CCTCAACCTC 
AAACATAATG GCATTTCTCA CTTGGATGAT GGATTTCTAA TGGGTGCAAA AAGACTTCAG 
GTTCTCAATC TCAAATCCAA CCAACTCACC ACCATTAACG AATCCACGTT CCAGCCCAGA 
CCTGAGAATC AGTTCCAGAC TTTGTACCTG GAGGAAAACC CCTTTCAGTG TACATGTGAT 
TTACTAGATT TCATCTTATG GATCGAAAAC AGTGACGTAA AAATCCCCGG GCTCGCCACG 
GATGTGAAAT GTGATGCACC TGCAAATCAA AAGGGTCGTG TTCTGATTAA GTTTGACATT 
ACTCAGTGTG TAAACAACAG TGAGGCATTC TTGATCTACA TCCTTACCAG TTCCTTTGTT 
ATTGGTTTTA TGCTTGTGAC CACTGTGGCC CATCTTTTTT ACTGGGATGC CACCTATGTC 
CTACACTATA TGAAAGCTAA GTTAAAGGGA TACAGCTCTT TGAACTCATC AGAGATTTTG 
TATGGTGCTT TTGTGACCTA CGACACCAGA GATCCACATG TCTCTGAATG GGTGATGAAG 
AACCTTCTGG TGAAACTAGA GGAGGAAGGA GAGAAGAACC TTCCTCTGTG TCTGGAGGAG 
AGGGACTGGA CCCCCGGAGT ACCGCTGGTA GACAACCTCA CTCAGAGCAT CCGATACAGT 
CGGAAGACCC TATTTGTCCT GACGCAGGAT TACGTCAAGA CCGGGATTTT CAAGATGGCC 
ATGTATCTGG CACACCAGAG ACTGCTTGAT GAAAATGTGG ATGTGATTGT TTTGCTGTTG 
CTGGAACCTG TTCTGCAGCA TTCTCACTTC CTTCGTCTGC GACGGAGACT ATGCGGTGAA 
AGTGTAGTGG ACTGGCCGAG AACAGCTGCC GCAGAACCCT GGTTTTGGCA AAACCTGAGG 
AATGTGGTCA GGGTGGAAAA TCAGGTTATG TACAACAAAA ATTATTCAAA GTACTTCACA 
AGTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961868.2
CDS	184..3252
Translation	MTLAWMLLSCLSWLSELHHAACKPTWLLPQFPCQVTGYNSSSVFFDCSRRHLHQVPKGITSNVTDLNLSENSIKEISPLAFSTMWNLTKLNLSWANKKTGVNISNNLFRNLTKLQHLRLSGNGMYRIPGNIPVSVRILKMGNNKITSLDAHSLAGLKNVTHFSLSRNCYRWNPCGKHVSIEKDSFSSMSNLVFLDLSFNNLSQVPKGLPQSLQFLKLDNNKIQNIYSDDFYGLQNLKELKLEGNCPRCENAPFPCDTCQNISVRIHPNAFYNLTNLETLNLGGNSLKELNPSWFERLYKLKQLFLAFNFLLKPITEQGAFLKYIPNIKKMDLSFNYNLRSYPKTINLSPEFSNLAALQTLHLEGLVFQNIGPDTLRPLYQLKNLSVLNLGTNFIIQADSSIFKKLSHLKMIYLGENRLYPIPEKNMPSLSRQYNQRLDFSVSSYMKPHGRDYFFKISQTLIKQECFDAGKVLSLNSNNFLFISTKEFEGDIKCLNLSRNGFAPALNGTEFSFLPNLTYLDLSFNKIDLAYSLAFNDLKKLQVLDLSYNPHYFNVQGITHKVNFLRNLPVLRVLNMSHNDISTLTTKYMESKSLAELRFTHNYLGTLWKENDLSYKKLFTKLTNLTILDISFNQIIKIPDEMYKHLPQNLTTLIISHNFLTDFKWNKLIFLPQIKVLDLSFNRLTNVTGIHIAHTLTLLNLKHNGISHLDDGFLMGAKRLQVLNLKSNQLTTINESTFQPRPENQFQTLYLEENPFQCTCDLLDFILWIENSDVKIPGLATDVKCDAPANQKGRVLIKFDITQCVNNSEAFLIYILTSSFVIGFMLVTTVAHLFYWDATYVLHYMKAKLKGYSSLNSSEILYGAFVTYDTRDPHVSEWVMKNLLVKLEEEGEKNLPLCLEERDWTPGVPLVDNLTQSIRYSRKTLFVLTQDYVKTGIFKMAMYLAHQRLLDENVDVIVLLLLEPVLQHSHFLRLRRRLCGESVVDWPRTAAAEPWFWQNLRNVVRVENQVMYNKNYSKYFTSK

Target ORF information:

RefSeq Version	XM_003961819.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes toll-like receptor 8 (LOC101062825), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961819.2

ORF Insert Sequence:

ATGACTTTGG CCTGGATGCT GCTGTCGTGT CTGTCGTGGC TTTCTGAGCT CCATCATGCT 
GCATGTAAAC CTACATGGCT GCTGCCACAG TTCCCCTGCC AAGTCACAGG ATACAACTCT 
TCCAGTGTCT TCTTCGACTG CTCAAGGCGC CACCTACATC AAGTCCCCAA AGGTATAACG 
AGCAACGTAA CCGATCTCAA CCTGTCAGAA AATTCAATCA AGGAGATTTC GCCTCTTGCA 
TTTTCCACAA TGTGGAACCT GACCAAGCTC AACCTCAGCT GGGCAAACAA GAAAACGGGG 
GTGAACATCA GCAATAATCT GTTCAGAAAT CTGACAAAGC TGCAGCATCT GCGCCTGAGT 
GGCAATGGCA TGTATAGAAT TCCTGGAAAC ATCCCTGTTA GCGTGAGGAT CCTTAAGATG 
GGCAACAACA AGATCACCTC TCTGGATGCA CACAGCCTTG CTGGCTTGAA AAATGTGACT 
CATTTTTCTT TGTCAAGAAA CTGCTACCGT TGGAATCCTT GTGGGAAACA TGTTAGCATT 
GAGAAGGACA GCTTTTCCAG CATGTCCAAC TTGGTGTTTC TGGACTTGTC CTTTAACAAT 
TTATCTCAGG TCCCGAAAGG ATTACCCCAA TCATTGCAAT TCCTAAAGTT AGATAACAAT 
AAAATACAAA ACATATACTC AGATGATTTT TATGGATTAC AGAATTTAAA AGAGCTCAAA 
CTAGAAGGCA ACTGCCCAAG GTGTGAAAAT GCTCCATTTC CATGTGATAC TTGTCAGAAT 
ATATCTGTCA GGATCCATCC TAATGCATTC TATAATTTGA CAAATTTGGA AACACTGAAC 
CTGGGAGGAA ATTCACTGAA AGAGCTGAAT CCCTCTTGGT TTGAGAGACT TTACAAGCTC 
AAACAATTGT TCCTTGCATT CAACTTTCTG TTAAAACCAA TTACTGAGCA GGGGGCATTC 
CTAAAATACA TCCCCAATAT AAAAAAAATG GATCTATCTT TTAACTATAA CCTAAGGTCC 
TATCCTAAAA CCATCAATCT TTCTCCAGAG TTTTCAAATC TGGCAGCCCT TCAAACATTA 
CATCTGGAGG GCTTGGTCTT CCAAAATATT GGACCGGACA CACTTCGACC ACTGTACCAG 
CTCAAAAATC TGTCTGTGCT AAACCTGGGG ACTAACTTCA TTATTCAGGC TGATTCTAGC 
ATATTTAAAA AACTATCTCA TCTAAAAATG ATATATCTTG GAGAAAACAG GCTGTATCCC 
ATTCCAGAGA AAAATATGCC GAGTCTGAGC AGGCAGTACA ACCAGAGGTT GGACTTTTCC 
GTTTCATCAT ACATGAAACC CCACGGAAGA GATTATTTCT TCAAGATATC TCAGACACTT 
ATCAAGCAAG AGTGCTTCGA TGCTGGAAAA GTGCTGAGCC TTAACTCGAA TAACTTCCTC 
TTTATTTCCA CAAAGGAGTT TGAAGGAGAT ATTAAGTGTC TCAATCTCTC GAGAAATGGA 
TTCGCACCAG CGCTCAATGG GACAGAGTTT TCCTTCCTGC CTAATTTGAC GTACTTAGAT 
CTGTCATTCA ATAAAATTGA TCTGGCCTAC AGCTTGGCAT TCAACGACCT AAAGAAGCTA 
CAAGTGCTGG ACCTCAGTTA CAATCCTCAC TACTTTAATG TACAAGGGAT AACCCATAAA 
GTTAACTTCT TAAGAAACCT GCCGGTCCTC AGGGTGCTGA ATATGAGCCA CAATGACATT 
TCCACACTGA CTACAAAATA CATGGAAAGC AAGTCTTTAG CGGAGCTTCG CTTCACACAC 
AATTATCTTG GGACTCTTTG GAAAGAAAAT GATCTTTCCT ACAAGAAACT TTTTACTAAA 
CTTACTAATT TGACCATTTT AGACATATCC TTCAACCAAA TTATAAAGAT CCCTGATGAG 
ATGTATAAAC ATTTGCCACA GAACCTCACC ACACTGATCA TAAGTCACAA CTTCCTGACT 
GATTTTAAAT GGAACAAGCT TATATTTTTA CCACAGATTA AAGTTTTAGA CCTCAGCTTC 
AACCGTTTGA CCAACGTGAC AGGTATACAC ATTGCCCACA CCCTGACACT CCTCAACCTC 
AAACATAATG GCATTTCTCA CTTGGATGAT GGATTTCTAA TGGGTGCAAA AAGACTTCAG 
GTTCTCAATC TCAAATCCAA CCAACTCACC ACCATTAACG AATCCACGTT CCAGCCCAGA 
CCTGAGAATC AGTTCCAGAC TTTGTACCTG GAGGAAAACC CCTTTCAGTG TACATGTGAT 
TTACTAGATT TCATCTTATG GATCGAAAAC AGTGACGTAA AAATCCCCGG GCTCGCCACG 
GATGTGAAAT GTGATGCACC TGCAAATCAA AAGGGTCGTG TTCTGATTAA GTTTGACATT 
ACTCAGTGTG TAAACAACAG TGAGGCATTC TTGATCTACA TCCTTACCAG TTCCTTTGTT 
ATTGGTTTTA TGCTTGTGAC CACTGTGGCC CATCTTTTTT ACTGGGATGC CACCTATGTC 
CTACACTATA TGAAAGCTAA GTTAAAGGGA TACAGCTCTT TGAACTCATC AGAGATTTTG 
TATGGTGCTT TTGTGACCTA CGACACCAGA GATCCACATG TCTCTGAATG GGTGATGAAG 
AACCTTCTGG TGAAACTAGA GGAGGAAGGA GAGAAGAACC TTCCTCTGTG TCTGGAGGAG 
AGGGACTGGA CCCCCGGAGT ACCGCTGGTA GACAACCTCA CTCAGAGCAT CCGATACAGT 
CGGAAGACCC TATTTGTCCT GACGCAGGAT TACGTCAAGA CCGGGATTTT CAAGATGGCC 
ATGTATCTGG CACACCAGAG ACTGCTTGAT GAAAATGTGG ATGTGATTGT TTTGCTGTTG 
CTGGAACCTG TTCTGCAGCA TTCTCACTTC CTTCGTCTGC GACGGAGACT ATGCGGTGAA 
AGTGTAGTGG ACTGGCCGAG AACAGCTGCC GCAGAACCCT GGTTTTGGCA AAACCTGAGG 
AATGTGGTCA GGGTGGAAAA TCAGGTTATG TACAACAAAA ATTATTCAAA GTACTTCACA 
AGTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101062825 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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