med1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	med1
Entrez Gene ID	101062821
Full Name	mediator complex subunit 1
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003961249.2	XP_003961298.1	mediator of RNA polymerase II transcription subunit 1 isoform X1
XM_011621868.1	XP_011620170.1	mediator of RNA polymerase II transcription subunit 1 isoform X2
XM_003961249.3	XP_003961298.2	mediator of RNA polymerase II transcription subunit 1

Pathways from BioSystems
Gene Ontology

The following med1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo00299	XM_003961249.2 Latest version!	Takifugu rubripes mediator complex subunit 1 (med1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo19902	XM_011621868.1 Latest version!	Takifugu rubripes mediator complex subunit 1 (med1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo42077	XM_003961249.3 Latest version!	Takifugu rubripes mediator complex subunit 1 (med1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med1 ( XM_003961249.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003961298.1 Takifugu rubripes mediator complex subunit 1 (med1), transcript variant X1, mRNA.

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CloneID	OTo00299
Clone ID Related Accession (Same CDS sequence)	XM_003961249.2
Accession Version	XM_003961249.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003961249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGCCTGGTGT TGCTCTGCAG TGTGATAGCC CTGCAAGAGA GCTCTCACTT 
TCCGTTCATC AAGGAAGTAA TCAGATTCAT GGAGATCGAA TCAAAACTGA AGAGTCAGCA 
GAGGAGGAGA AGGAAAGTCG CTCAGCTGTT TTGCTGGAGA AGCTTCACGC CAAGTATATT 
GCTTCAAGGC CATGGCAGGA GACCTTCAAA CTAGTGCGTC AGGCCATGGA CAAACGAGGT 
CCAACAAATG CAGCAAGTCA CCAGTTGATG CACAGCTGCT TAGAGAACTT GCAGAGAGCG 
ATGAAAGTTT CATCTTTGTC TGCCATGACA GACCGTTTAG AGTCCATCGC TCGGCAAAAC 
ATGCTGGGCT CTCACCTCAG CCCGTCAGGG ACAGAATGTT ACATCACGTC AGACATGTTT 
TATGTGGAGG TGCAGCTGGA CAACGGTGGT CAGCTAGTAG ATGTCAAGGT GGCTCATCAG 
GGAGAAAACC CTACAAGTTG CCTGGAGCTC ATTCAGCATC TTCGGGAGAA GAACTTTGAT 
GAGTTTTCAA AACATCTGAA GGGCCTGGTT GAACTATACA AGCTCCCCGG TGATAATAAG 
TTAAAAACCA AGATGTACAT TGCTCTACAG TCTTTGGAGC TTGATCTCAC CAAGATGATG 
CACATGTACA GGTTAGCAAC CAGTGCTAAT ACACTGGAGA CTCTTCTTCA TGGCAGCGTA 
GGCTTGTTGT CTGCAAGGAG CGGTGGTCAT CTTGTTTCTC TTCACTGTTA CCTCTCACCA 
TATGATTTAC TGGAGGAAAG AACCTGTGCG CCACTCAATT TAACAGACAA CAATGTTCCG 
CGTTCTCTGG GTGTGAGCGT TTCTGTGACA ATTGAGGGAA CCTCTGCTGT TTACAAACTC 
CCGATCGCTC CACTCATCAC TGGATCACAT CCCGTGGATA ACAAGGGGAC ACCTTCATTT 
TCCACTGTGA CTAATTCCAA CTGTGTGGAC CTGCCAGCTT GTTTTTTCCT CAAGCTGAAT 
CGTCCCATGC CTTTCTCAGT GGCCTTCATT CAGAGGATGG GCAATGCTAC ATCTATTCCA 
GTTTTTGAGA GCCCACCCAC TCTCTCGCCT CTTTACCAAT TAATTGTCCA GAGTGAGCTT 
CGCCTCCAAG AAGATGGGAG TGTCACACCA CCGTGTCCAC ACAACATGAC CTTCTACTCA 
GTATTGCCAG GGCAGCTGCA CTGTTATTTT TTGAATGGTG ACGCCCCAGT GCAAGATGGT 
CAGTCTCTTC AAGGGGCAAT CGTGTCCAAG ATCCCCTTCC GCCACCCAGC CCAGGTCCCA 
CTTCTGTTAG ATATCGTTCG GCACCAGGCA GCTTATAATT CCTTGATTAG CAGCTGTGTG 
AAACGCACTT CAGTCGACGA AGATGGCGCA GGCCTGCTGC AGTTTGAAGT GTGTCCCCTG 
ACTGACTCCA GTTTTAGTGT CTCGTTCCAG CATCCAGTCA ATGAGTCGTT GGTTTGCGTT 
GTCATGGATG TGATCGACTC CAGACAGGTC TCCTGCAAGT TATATAAAGG ACCTTCGGAT 
GCATTAATTT GTACTGATGA TTTCATCACC AAAGTTGTTC ACAGATGCAT GTCCATCCCA 
GTAACAATGC GGGCTATTCG GAGAAAAGCG GAGACCATCC AGGCAGACAC TCCCGCCCTG 
TCACTTATTG CACAGACGGT GGAGATCATG GTGAAGAACA ACCTCCCACC CTCGGGAAGT 
CCTACATATA ACATGGCGTC ATGTGATGGA CCAAACCCGA TGGGACTGCC TGGGCTCACT 
GGGGGCAATA CGCCCACTGG AGGTGGACCA CCTGGAGGAC CCAATTTTGC TGGCCCAATT 
ACTTCTCTGT TTGGAATATC CCGGATGGAA AGGCAAGCTC AAGGAGGAGA GTGTCCCTCA 
CAACCCGGGG GAACTGGCCA GCAACAATTG CAACAGCATC AGCAACAGCA GGCAGGTCAA 
GGCCATTCAG AAGACTACAA CAAAGTCACA CAGAATCCCA TATTGACAAG TTTGCTGCAG 
ATAACCGGAA GTGTTGGCTC TAGTCCCAAC TCCCAAAATG CACCAACACC CCACCAGACC 
CCACCTCCAA CATCGTCCCC TGCGAGCAAC ACGAAAAATC ATCCAATGTT GATGAATTTG 
TTGAAGGACA ACCCTGCGCA AGATTTTGCC GCACTGTATG GTTCTAGTCC ATTAGAGCGG 
CAGAATTCTT CATCCGGATC TCCACGGACG GAGGGCATGG CTGGAACATG CCCTGGAGGT 
AGCCAAAAGG TTAAAAAGAA ACGTCCAAGG GTGGTGGAAA AGGGCAGCAT GTTGCCCGGT 
GCTTCTGCTA GTGGTGGTAG CGGTTTAAGC ATGAAGTCCC AACAGTCTTC CTCGATGTCA 
CAGCAGCACC AACACCACCC TGTCTCGCAT GAAGACGATT TCCACCGTGA GCTTCTCTCT 
ATGGATGTAG ATGCTTCTCA GAATTCGATA TTTGACGTTA ACCTCACCGG TGATGGCCTC 
GACACACCAC ATAGCATCAC TCCAGCACCC AGCCAATGTG GAACGCCACC AGCTGGCCCC 
AACATGCCGT ACTCGCAGTC CCACGTCCAA TCCCAGCAAC AGCAACCCCA AGGTGCAGCA 
CCGTCCCATA TGGTCCGCTT GTCTAGTTCT GACAGCATTG GGCCTGATAT CACAGAAATT 
TTGTCAGACC TGCCCGAGCA GACAGGAAAA GGCAGCAGTG GAAGCCATGG ACAGCACCCC 
ATTGGCAGCA GCGGAGAGGA AGGAGGCCCT CTCGGTACCC CGATACGTGA CTCCTCGAGC 
TCAGGTCAGG GTAGTGCAGT TTTTGACTCG GCGGATATTT TCAACACGAA CAGCAACGAG 
AATCCTTTTA CCGATGCCGC TGACTTGATC GCAGAGGCGG CCGCAACTGC TGCTACTCCC 
ACCAGTGATT CCTCTTCGAA CCATTTCTTC CCCGACGCTG CCGACTTCAA TGCTGACCTC 
CTGACTTCAG GCCATGGCTT CTCGCAGAAC TACTTTGATG ACAGCTCGCC GAGTGCAGAC 
GGAGATATGG ATCTGGTCAA AGGGTTTGGG GGGAGCAGCC AGCAAAACAC TCCATCAGGA 
ACGCCTCAGA ATCCAACGTC ACATGGGCAA AGCACCCCAG AGCCGTCGCT GAAAGATCCG 
TTCGATATGG GGATCGTTTT TGGAGGTAAC AGTGGTGGCG GCAAGCCACT TTTAGGGCAA 
GCCTCTGATA TGTCAGATAC GCATAGTGGA GGGTCACAGA GCCCTCTTAT AATGGGAGCA 
GGATGTGGGG ATTTTAAGGG GGCAGAACCC AAAGTGAAAC AGCAACAGAG TCACATGCGG 
CCAAAGGATG AGAATGGGGG TAGCAGTGCG GGTAATGCTG TGGTAGGGAT GGGGAGTAGT 
TCAACAGAGT GCAAACAAAT CAAACGAAGC AGGACCCCAT CCAGTGAGGG GAAGTGCAAA 
GAAAAGCCTC CCAAACGTAA AAAGGTGGAC TCGGATGGGA AATCGCCCTC TCACAGTTCT 
GGGGGACGAC CATATACGCC CCCTAGTGGT GGCTCTGGGT CTGGAGGAAG CATGAGTGGG 
GGAGGCTCTA AATCTCCCGG TAGTTCAGGG CGCTCACAAA CGCCCCCTGG TGGTGCCACG 
CCGCCGATTC CCAAAATAAC TATTCAAATA ACAAAAGGGA CTATAACTGG GGGGAAAACA 
TCATCTCTTA GTGGATACAC CTCCAGCAGC TCAGCCACAG GTGGCACAGG AGCAACAGGG 
GGAAACAGTG GCAGCAAAAG TCATCATTCG CACTCCTCCT CCTCCTCTGG GAAGATCAAG 
TCCAAGGAAA GTTCTGCTAC ACCAGGCAGC GCGTCAAAGC CGGGCAGTGC GGGAATAACA 
AGTGGAGTGC CATCCCAGTC TAAAACTCCT TCACAAGGAA TAGGCATTGG TAAACCTGGA 
TCCTCCCCAA TTAATAAACA TGGTTTGTCG GGGCCTGGAA GCAGCGGCAG TGGAATTGTG 
GGAGGCAATA AGACAAAACC TCCAGGGGGC AAGCCTCCTG GGTCTCTTAT GAATCCCAAC 
ATTAAGCCCA ACATATCCCC TTCTCACTCC CGCTCCAGTA GCTCTGGTGA CAAAATGTCT 
TCCCCACTGA AAATGCAGCA AGCCCAAGTT CCAGGAACAC CCCCTTCCTC TAAAGCGAAA 
TCACCAATTG GCTCAGGTGG CAGCAGTTCC GCAGGGTCCA AATTATCCTC CGGCCCAGGC 
ATGAGCTCCC AGAAACCGCT GGGTGGGGGC TCCTCCTCTG GATCCACATC TTCATCATCG 
TCTTCCAGCT CAACATCATC ATCCGGCTCC ATGACTTTTT CAGGAAGCTC GCAGTCTCAG 
TACGGGGGAG GTGGCAGTGG AGGAGGAGGT GGTAGCGCGG CAGGAGGAAG CGGATGTGGA 
GGCGGTGGTA GCAGTGGAGG AAGCGGAGGT GGCACAGTAG CCGGTCAGAA CAATGCAAAC 
AACCCCAACG CCAAGGGAAA ATCCCCCAGT CGAAACAAGA AACCCTCTCT CACAGCAGTC 
ATAGACAAAT TAAAAAGTGT AGGTGGAGGA GGAGTTGGGG AGGATGGTTG TGAGGTTGGG 
CCACCTGTAG GTGGTCCTGG TTCTGGAAGT GTTCCTAGTG GTGGTGGACC TGGAAATGTT 
CCTAGTGGTG GAGCGCTTAA CATATGTCCT TCTAAACACA TCTCAACCTC TCAAGGTGGT 
GAGTATAAGA GGGAGAAGTC TGAAAAGGAA GCAAAAGCAA AAGTGTCTGT TTCAGGGGGG 
AACAGCGGGG ATAAAAAGCT GATGGACCCC AAAACGGGAG GTGTGGGTAC CACTGGTTTG 
GCTAAAATTA TCATTAGCAA ACCTGATGGT GGCTCACCAA GCATTAAGGC CAAAGGGCCT 
CTCCAGAAAT CAGGAGAAGG GTCCGGTGAC TCAATTCGCC CCCAGATGAC TAGCCTCAAA 
GCTTCCCCCC TTTTCAGTGG CTCAACCCCC AAGCACGACC GCTCCTCTCC GAGCCACAGC 
CGCTCACCTG GATGCACCCC GCTCAACCAC GACAGCGAGA GCGAGTCGGG CAGCAGCTCG 
GTGGCCGAGA AATCCCACCA GAACAGTCCC AGCTCAGACG ACGACCAGAC TATCCGACCG 
CTTCCACCTC AGGACTACTT GAGCTCTATT CCTCTCAGCT CAGGGGAGAA GCACAAGAAA 
CACAAGAAGG AAAAGAAAAA GAAAGAGCGG GAACGGGAAA GAGACAGAGA TCGGGAGAGG 
GACCGGGACA AAGAGAAAAA GAAATCATCC ATGTCCATGG TCCCGTCCTC GCATCCCATC 
AAAGCAGACA GTTGGTCCCG GTCACCCATC TCTGCTTCAG ATTCTTCTTT GTCCATGCTG 
GGTCCAGAGC GTCCCACCCG GCCCAGCCCA TTGTATATGA GAAATGAAGA CGATGACCTC 
ATGGACTCAG CTCTGACTGG CAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961298.1
CDS	32..5521
Translation	MAAGPGVALQCDSPARELSLSVHQGSNQIHGDRIKTEESAEEEKESRSAVLLEKLHAKYIASRPWQETFKLVRQAMDKRGPTNAASHQLMHSCLENLQRAMKVSSLSAMTDRLESIARQNMLGSHLSPSGTECYITSDMFYVEVQLDNGGQLVDVKVAHQGENPTSCLELIQHLREKNFDEFSKHLKGLVELYKLPGDNKLKTKMYIALQSLELDLTKMMHMYRLATSANTLETLLHGSVGLLSARSGGHLVSLHCYLSPYDLLEERTCAPLNLTDNNVPRSLGVSVSVTIEGTSAVYKLPIAPLITGSHPVDNKGTPSFSTVTNSNCVDLPACFFLKLNRPMPFSVAFIQRMGNATSIPVFESPPTLSPLYQLIVQSELRLQEDGSVTPPCPHNMTFYSVLPGQLHCYFLNGDAPVQDGQSLQGAIVSKIPFRHPAQVPLLLDIVRHQAAYNSLISSCVKRTSVDEDGAGLLQFEVCPLTDSSFSVSFQHPVNESLVCVVMDVIDSRQVSCKLYKGPSDALICTDDFITKVVHRCMSIPVTMRAIRRKAETIQADTPALSLIAQTVEIMVKNNLPPSGSPTYNMASCDGPNPMGLPGLTGGNTPTGGGPPGGPNFAGPITSLFGISRMERQAQGGECPSQPGGTGQQQLQQHQQQQAGQGHSEDYNKVTQNPILTSLLQITGSVGSSPNSQNAPTPHQTPPPTSSPASNTKNHPMLMNLLKDNPAQDFAALYGSSPLERQNSSSGSPRTEGMAGTCPGGSQKVKKKRPRVVEKGSMLPGASASGGSGLSMKSQQSSSMSQQHQHHPVSHEDDFHRELLSMDVDASQNSIFDVNLTGDGLDTPHSITPAPSQCGTPPAGPNMPYSQSHVQSQQQQPQGAAPSHMVRLSSSDSIGPDITEILSDLPEQTGKGSSGSHGQHPIGSSGEEGGPLGTPIRDSSSSGQGSAVFDSADIFNTNSNENPFTDAADLIAEAAATAATPTSDSSSNHFFPDAADFNADLLTSGHGFSQNYFDDSSPSADGDMDLVKGFGGSSQQNTPSGTPQNPTSHGQSTPEPSLKDPFDMGIVFGGNSGGGKPLLGQASDMSDTHSGGSQSPLIMGAGCGDFKGAEPKVKQQQSHMRPKDENGGSSAGNAVVGMGSSSTECKQIKRSRTPSSEGKCKEKPPKRKKVDSDGKSPSHSSGGRPYTPPSGGSGSGGSMSGGGSKSPGSSGRSQTPPGGATPPIPKITIQITKGTITGGKTSSLSGYTSSSSATGGTGATGGNSGSKSHHSHSSSSSGKIKSKESSATPGSASKPGSAGITSGVPSQSKTPSQGIGIGKPGSSPINKHGLSGPGSSGSGIVGGNKTKPPGGKPPGSLMNPNIKPNISPSHSRSSSSGDKMSSPLKMQQAQVPGTPPSSKAKSPIGSGGSSSAGSKLSSGPGMSSQKPLGGGSSSGSTSSSSSSSSTSSSGSMTFSGSSQSQYGGGGSGGGGGSAAGGSGCGGGGSSGGSGGGTVAGQNNANNPNAKGKSPSRNKKPSLTAVIDKLKSVGGGGVGEDGCEVGPPVGGPGSGSVPSGGGPGNVPSGGALNICPSKHISTSQGGEYKREKSEKEAKAKVSVSGGNSGDKKLMDPKTGGVGTTGLAKIIISKPDGGSPSIKAKGPLQKSGEGSGDSIRPQMTSLKASPLFSGSTPKHDRSSPSHSRSPGCTPLNHDSESESGSSSVAEKSHQNSPSSDDDQTIRPLPPQDYLSSIPLSSGEKHKKHKKEKKKKERERERDRDRERDRDKEKKKSSMSMVPSSHPIKADSWSRSPISASDSSLSMLGPERPTRPSPLYMRNEDDDLMDSALTGNI

Target ORF information:

RefSeq Version	XM_003961249.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mediator complex subunit 1 (med1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961249.2

ORF Insert Sequence:

ATGGCGGCGG GGCCTGGTGT TGCTCTGCAG TGTGATAGCC CTGCAAGAGA GCTCTCACTT 
TCCGTTCATC AAGGAAGTAA TCAGATTCAT GGAGATCGAA TCAAAACTGA AGAGTCAGCA 
GAGGAGGAGA AGGAAAGTCG CTCAGCTGTT TTGCTGGAGA AGCTTCACGC CAAGTATATT 
GCTTCAAGGC CATGGCAGGA GACCTTCAAA CTAGTGCGTC AGGCCATGGA CAAACGAGGT 
CCAACAAATG CAGCAAGTCA CCAGTTGATG CACAGCTGCT TAGAGAACTT GCAGAGAGCG 
ATGAAAGTTT CATCTTTGTC TGCCATGACA GACCGTTTAG AGTCCATCGC TCGGCAAAAC 
ATGCTGGGCT CTCACCTCAG CCCGTCAGGG ACAGAATGTT ACATCACGTC AGACATGTTT 
TATGTGGAGG TGCAGCTGGA CAACGGTGGT CAGCTAGTAG ATGTCAAGGT GGCTCATCAG 
GGAGAAAACC CTACAAGTTG CCTGGAGCTC ATTCAGCATC TTCGGGAGAA GAACTTTGAT 
GAGTTTTCAA AACATCTGAA GGGCCTGGTT GAACTATACA AGCTCCCCGG TGATAATAAG 
TTAAAAACCA AGATGTACAT TGCTCTACAG TCTTTGGAGC TTGATCTCAC CAAGATGATG 
CACATGTACA GGTTAGCAAC CAGTGCTAAT ACACTGGAGA CTCTTCTTCA TGGCAGCGTA 
GGCTTGTTGT CTGCAAGGAG CGGTGGTCAT CTTGTTTCTC TTCACTGTTA CCTCTCACCA 
TATGATTTAC TGGAGGAAAG AACCTGTGCG CCACTCAATT TAACAGACAA CAATGTTCCG 
CGTTCTCTGG GTGTGAGCGT TTCTGTGACA ATTGAGGGAA CCTCTGCTGT TTACAAACTC 
CCGATCGCTC CACTCATCAC TGGATCACAT CCCGTGGATA ACAAGGGGAC ACCTTCATTT 
TCCACTGTGA CTAATTCCAA CTGTGTGGAC CTGCCAGCTT GTTTTTTCCT CAAGCTGAAT 
CGTCCCATGC CTTTCTCAGT GGCCTTCATT CAGAGGATGG GCAATGCTAC ATCTATTCCA 
GTTTTTGAGA GCCCACCCAC TCTCTCGCCT CTTTACCAAT TAATTGTCCA GAGTGAGCTT 
CGCCTCCAAG AAGATGGGAG TGTCACACCA CCGTGTCCAC ACAACATGAC CTTCTACTCA 
GTATTGCCAG GGCAGCTGCA CTGTTATTTT TTGAATGGTG ACGCCCCAGT GCAAGATGGT 
CAGTCTCTTC AAGGGGCAAT CGTGTCCAAG ATCCCCTTCC GCCACCCAGC CCAGGTCCCA 
CTTCTGTTAG ATATCGTTCG GCACCAGGCA GCTTATAATT CCTTGATTAG CAGCTGTGTG 
AAACGCACTT CAGTCGACGA AGATGGCGCA GGCCTGCTGC AGTTTGAAGT GTGTCCCCTG 
ACTGACTCCA GTTTTAGTGT CTCGTTCCAG CATCCAGTCA ATGAGTCGTT GGTTTGCGTT 
GTCATGGATG TGATCGACTC CAGACAGGTC TCCTGCAAGT TATATAAAGG ACCTTCGGAT 
GCATTAATTT GTACTGATGA TTTCATCACC AAAGTTGTTC ACAGATGCAT GTCCATCCCA 
GTAACAATGC GGGCTATTCG GAGAAAAGCG GAGACCATCC AGGCAGACAC TCCCGCCCTG 
TCACTTATTG CACAGACGGT GGAGATCATG GTGAAGAACA ACCTCCCACC CTCGGGAAGT 
CCTACATATA ACATGGCGTC ATGTGATGGA CCAAACCCGA TGGGACTGCC TGGGCTCACT 
GGGGGCAATA CGCCCACTGG AGGTGGACCA CCTGGAGGAC CCAATTTTGC TGGCCCAATT 
ACTTCTCTGT TTGGAATATC CCGGATGGAA AGGCAAGCTC AAGGAGGAGA GTGTCCCTCA 
CAACCCGGGG GAACTGGCCA GCAACAATTG CAACAGCATC AGCAACAGCA GGCAGGTCAA 
GGCCATTCAG AAGACTACAA CAAAGTCACA CAGAATCCCA TATTGACAAG TTTGCTGCAG 
ATAACCGGAA GTGTTGGCTC TAGTCCCAAC TCCCAAAATG CACCAACACC CCACCAGACC 
CCACCTCCAA CATCGTCCCC TGCGAGCAAC ACGAAAAATC ATCCAATGTT GATGAATTTG 
TTGAAGGACA ACCCTGCGCA AGATTTTGCC GCACTGTATG GTTCTAGTCC ATTAGAGCGG 
CAGAATTCTT CATCCGGATC TCCACGGACG GAGGGCATGG CTGGAACATG CCCTGGAGGT 
AGCCAAAAGG TTAAAAAGAA ACGTCCAAGG GTGGTGGAAA AGGGCAGCAT GTTGCCCGGT 
GCTTCTGCTA GTGGTGGTAG CGGTTTAAGC ATGAAGTCCC AACAGTCTTC CTCGATGTCA 
CAGCAGCACC AACACCACCC TGTCTCGCAT GAAGACGATT TCCACCGTGA GCTTCTCTCT 
ATGGATGTAG ATGCTTCTCA GAATTCGATA TTTGACGTTA ACCTCACCGG TGATGGCCTC 
GACACACCAC ATAGCATCAC TCCAGCACCC AGCCAATGTG GAACGCCACC AGCTGGCCCC 
AACATGCCGT ACTCGCAGTC CCACGTCCAA TCCCAGCAAC AGCAACCCCA AGGTGCAGCA 
CCGTCCCATA TGGTCCGCTT GTCTAGTTCT GACAGCATTG GGCCTGATAT CACAGAAATT 
TTGTCAGACC TGCCCGAGCA GACAGGAAAA GGCAGCAGTG GAAGCCATGG ACAGCACCCC 
ATTGGCAGCA GCGGAGAGGA AGGAGGCCCT CTCGGTACCC CGATACGTGA CTCCTCGAGC 
TCAGGTCAGG GTAGTGCAGT TTTTGACTCG GCGGATATTT TCAACACGAA CAGCAACGAG 
AATCCTTTTA CCGATGCCGC TGACTTGATC GCAGAGGCGG CCGCAACTGC TGCTACTCCC 
ACCAGTGATT CCTCTTCGAA CCATTTCTTC CCCGACGCTG CCGACTTCAA TGCTGACCTC 
CTGACTTCAG GCCATGGCTT CTCGCAGAAC TACTTTGATG ACAGCTCGCC GAGTGCAGAC 
GGAGATATGG ATCTGGTCAA AGGGTTTGGG GGGAGCAGCC AGCAAAACAC TCCATCAGGA 
ACGCCTCAGA ATCCAACGTC ACATGGGCAA AGCACCCCAG AGCCGTCGCT GAAAGATCCG 
TTCGATATGG GGATCGTTTT TGGAGGTAAC AGTGGTGGCG GCAAGCCACT TTTAGGGCAA 
GCCTCTGATA TGTCAGATAC GCATAGTGGA GGGTCACAGA GCCCTCTTAT AATGGGAGCA 
GGATGTGGGG ATTTTAAGGG GGCAGAACCC AAAGTGAAAC AGCAACAGAG TCACATGCGG 
CCAAAGGATG AGAATGGGGG TAGCAGTGCG GGTAATGCTG TGGTAGGGAT GGGGAGTAGT 
TCAACAGAGT GCAAACAAAT CAAACGAAGC AGGACCCCAT CCAGTGAGGG GAAGTGCAAA 
GAAAAGCCTC CCAAACGTAA AAAGGTGGAC TCGGATGGGA AATCGCCCTC TCACAGTTCT 
GGGGGACGAC CATATACGCC CCCTAGTGGT GGCTCTGGGT CTGGAGGAAG CATGAGTGGG 
GGAGGCTCTA AATCTCCCGG TAGTTCAGGG CGCTCACAAA CGCCCCCTGG TGGTGCCACG 
CCGCCGATTC CCAAAATAAC TATTCAAATA ACAAAAGGGA CTATAACTGG GGGGAAAACA 
TCATCTCTTA GTGGATACAC CTCCAGCAGC TCAGCCACAG GTGGCACAGG AGCAACAGGG 
GGAAACAGTG GCAGCAAAAG TCATCATTCG CACTCCTCCT CCTCCTCTGG GAAGATCAAG 
TCCAAGGAAA GTTCTGCTAC ACCAGGCAGC GCGTCAAAGC CGGGCAGTGC GGGAATAACA 
AGTGGAGTGC CATCCCAGTC TAAAACTCCT TCACAAGGAA TAGGCATTGG TAAACCTGGA 
TCCTCCCCAA TTAATAAACA TGGTTTGTCG GGGCCTGGAA GCAGCGGCAG TGGAATTGTG 
GGAGGCAATA AGACAAAACC TCCAGGGGGC AAGCCTCCTG GGTCTCTTAT GAATCCCAAC 
ATTAAGCCCA ACATATCCCC TTCTCACTCC CGCTCCAGTA GCTCTGGTGA CAAAATGTCT 
TCCCCACTGA AAATGCAGCA AGCCCAAGTT CCAGGAACAC CCCCTTCCTC TAAAGCGAAA 
TCACCAATTG GCTCAGGTGG CAGCAGTTCC GCAGGGTCCA AATTATCCTC CGGCCCAGGC 
ATGAGCTCCC AGAAACCGCT GGGTGGGGGC TCCTCCTCTG GATCCACATC TTCATCATCG 
TCTTCCAGCT CAACATCATC ATCCGGCTCC ATGACTTTTT CAGGAAGCTC GCAGTCTCAG 
TACGGGGGAG GTGGCAGTGG AGGAGGAGGT GGTAGCGCGG CAGGAGGAAG CGGATGTGGA 
GGCGGTGGTA GCAGTGGAGG AAGCGGAGGT GGCACAGTAG CCGGTCAGAA CAATGCAAAC 
AACCCCAACG CCAAGGGAAA ATCCCCCAGT CGAAACAAGA AACCCTCTCT CACAGCAGTC 
ATAGACAAAT TAAAAAGTGT AGGTGGAGGA GGAGTTGGGG AGGATGGTTG TGAGGTTGGG 
CCACCTGTAG GTGGTCCTGG TTCTGGAAGT GTTCCTAGTG GTGGTGGACC TGGAAATGTT 
CCTAGTGGTG GAGCGCTTAA CATATGTCCT TCTAAACACA TCTCAACCTC TCAAGGTGGT 
GAGTATAAGA GGGAGAAGTC TGAAAAGGAA GCAAAAGCAA AAGTGTCTGT TTCAGGGGGG 
AACAGCGGGG ATAAAAAGCT GATGGACCCC AAAACGGGAG GTGTGGGTAC CACTGGTTTG 
GCTAAAATTA TCATTAGCAA ACCTGATGGT GGCTCACCAA GCATTAAGGC CAAAGGGCCT 
CTCCAGAAAT CAGGAGAAGG GTCCGGTGAC TCAATTCGCC CCCAGATGAC TAGCCTCAAA 
GCTTCCCCCC TTTTCAGTGG CTCAACCCCC AAGCACGACC GCTCCTCTCC GAGCCACAGC 
CGCTCACCTG GATGCACCCC GCTCAACCAC GACAGCGAGA GCGAGTCGGG CAGCAGCTCG 
GTGGCCGAGA AATCCCACCA GAACAGTCCC AGCTCAGACG ACGACCAGAC TATCCGACCG 
CTTCCACCTC AGGACTACTT GAGCTCTATT CCTCTCAGCT CAGGGGAGAA GCACAAGAAA 
CACAAGAAGG AAAAGAAAAA GAAAGAGCGG GAACGGGAAA GAGACAGAGA TCGGGAGAGG 
GACCGGGACA AAGAGAAAAA GAAATCATCC ATGTCCATGG TCCCGTCCTC GCATCCCATC 
AAAGCAGACA GTTGGTCCCG GTCACCCATC TCTGCTTCAG ATTCTTCTTT GTCCATGCTG 
GGTCCAGAGC GTCCCACCCG GCCCAGCCCA TTGTATATGA GAAATGAAGA CGATGACCTC 
ATGGACTCAG CTCTGACTGG CAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med1 ( XM_011621868.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011620170.1 Takifugu rubripes mediator complex subunit 1 (med1), transcript variant X2, mRNA.

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CloneID	OTo19902
Clone ID Related Accession (Same CDS sequence)	XM_011621868.1
Accession Version	XM_011621868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGCT GCTTAGAGAA CTTGCAGAGA GCGATGAAAG TTTCATCTTT GTCTGCCATG 
ACAGACCGTT TAGAGTCCAT CGCTCGGCAA AACATGCTGG GCTCTCACCT CAGCCCGTCA 
GGGACAGAAT GTTACATCAC GTCAGACATG TTTTATGTGG AGGTGCAGCT GGACAACGGT 
GGTCAGCTAG TAGATGTCAA GGTGGCTCAT CAGGGAGAAA ACCCTACAAG TTGCCTGGAG 
CTCATTCAGC ATCTTCGGGA GAAGAACTTT GATGAGTTTT CAAAACATCT GAAGGGCCTG 
GTTGAACTAT ACAAGCTCCC CGGTGATAAT AAGTTAAAAA CCAAGATGTA CATTGCTCTA 
CAGTCTTTGG AGCTTGATCT CACCAAGATG ATGCACATGT ACAGGTTAGC AACCAGTGCT 
AATACACTGG AGACTCTTCT TCATGGCAGC GTAGGCTTGT TGTCTGCAAG GAGCGGTGGT 
CATCTTGTTT CTCTTCACTG TTACCTCTCA CCATATGATT TACTGGAGGA AAGAACCTGT 
GCGCCACTCA ATTTAACAGA CAACAATGTT CCGCGTTCTC TGGGTGTGAG CGTTTCTGTG 
ACAATTGAGG GAACCTCTGC TGTTTACAAA CTCCCGATCG CTCCACTCAT CACTGGATCA 
CATCCCGTGG ATAACAAGGG GACACCTTCA TTTTCCACTG TGACTAATTC CAACTGTGTG 
GACCTGCCAG CTTGTTTTTT CCTCAAGCTG AATCGTCCCA TGCCTTTCTC AGTGGCCTTC 
ATTCAGAGGA TGGGCAATGC TACATCTATT CCAGTTTTTG AGAGCCCACC CACTCTCTCG 
CCTCTTTACC AATTAATTGT CCAGAGTGAG CTTCGCCTCC AAGAAGATGG GAGTGTCACA 
CCACCGTGTC CACACAACAT GACCTTCTAC TCAGTATTGC CAGGGCAGCT GCACTGTTAT 
TTTTTGAATG GTGACGCCCC AGTGCAAGAT GGTCAGTCTC TTCAAGGGGC AATCGTGTCC 
AAGATCCCCT TCCGCCACCC AGCCCAGGTC CCACTTCTGT TAGATATCGT TCGGCACCAG 
GCAGCTTATA ATTCCTTGAT TAGCAGCTGT GTGAAACGCA CTTCAGTCGA CGAAGATGGC 
GCAGGCCTGC TGCAGTTTGA AGTGTGTCCC CTGACTGACT CCAGTTTTAG TGTCTCGTTC 
CAGCATCCAG TCAATGAGTC GTTGGTTTGC GTTGTCATGG ATGTGATCGA CTCCAGACAG 
GTCTCCTGCA AGTTATATAA AGGACCTTCG GATGCATTAA TTTGTACTGA TGATTTCATC 
ACCAAAGTTG TTCACAGATG CATGTCCATC CCAGTAACAA TGCGGGCTAT TCGGAGAAAA 
GCGGAGACCA TCCAGGCAGA CACTCCCGCC CTGTCACTTA TTGCACAGAC GGTGGAGATC 
ATGGTGAAGA ACAACCTCCC ACCCTCGGGA AGTCCTACAT ATAACATGGC GTCATGTGAT 
GGACCAAACC CGATGGGACT GCCTGGGCTC ACTGGGGGCA ATACGCCCAC TGGAGGTGGA 
CCACCTGGAG GACCCAATTT TGCTGGCCCA ATTACTTCTC TGTTTGGAAT ATCCCGGATG 
GAAAGGCAAG CTCAAGGAGG AGAGTGTCCC TCACAACCCG GGGGAACTGG CCAGCAACAA 
TTGCAACAGC ATCAGCAACA GCAGGCAGGT CAAGGCCATT CAGAAGACTA CAACAAAGTC 
ACACAGAATC CCATATTGAC AAGTTTGCTG CAGATAACCG GAAGTGTTGG CTCTAGTCCC 
AACTCCCAAA ATGCACCAAC ACCCCACCAG ACCCCACCTC CAACATCGTC CCCTGCGAGC 
AACACGAAAA ATCATCCAAT GTTGATGAAT TTGTTGAAGG ACAACCCTGC GCAAGATTTT 
GCCGCACTGT ATGGTTCTAG TCCATTAGAG CGGCAGAATT CTTCATCCGG ATCTCCACGG 
ACGGAGGGCA TGGCTGGAAC ATGCCCTGGA GGTAGCCAAA AGGTTAAAAA GAAACGTCCA 
AGGGTGGTGG AAAAGGGCAG CATGTTGCCC GGTGCTTCTG CTAGTGGTGG TAGCGGTTTA 
AGCATGAAGT CCCAACAGTC TTCCTCGATG TCACAGCAGC ACCAACACCA CCCTGTCTCG 
CATGAAGACG ATTTCCACCG TGAGCTTCTC TCTATGGATG TAGATGCTTC TCAGAATTCG 
ATATTTGACG TTAACCTCAC CGGTGATGGC CTCGACACAC CACATAGCAT CACTCCAGCA 
CCCAGCCAAT GTGGAACGCC ACCAGCTGGC CCCAACATGC CGTACTCGCA GTCCCACGTC 
CAATCCCAGC AACAGCAACC CCAAGGTGCA GCACCGTCCC ATATGGTCCG CTTGTCTAGT 
TCTGACAGCA TTGGGCCTGA TATCACAGAA ATTTTGTCAG ACCTGCCCGA GCAGACAGGA 
AAAGGCAGCA GTGGAAGCCA TGGACAGCAC CCCATTGGCA GCAGCGGAGA GGAAGGAGGC 
CCTCTCGGTA CCCCGATACG TGACTCCTCG AGCTCAGGTC AGGGTAGTGC AGTTTTTGAC 
TCGGCGGATA TTTTCAACAC GAACAGCAAC GAGAATCCTT TTACCGATGC CGCTGACTTG 
ATCGCAGAGG CGGCCGCAAC TGCTGCTACT CCCACCAGTG ATTCCTCTTC GAACCATTTC 
TTCCCCGACG CTGCCGACTT CAATGCTGAC CTCCTGACTT CAGGCCATGG CTTCTCGCAG 
AACTACTTTG ATGACAGCTC GCCGAGTGCA GACGGAGATA TGGATCTGGT CAAAGGGTTT 
GGGGGGAGCA GCCAGCAAAA CACTCCATCA GGAACGCCTC AGAATCCAAC GTCACATGGG 
CAAAGCACCC CAGAGCCGTC GCTGAAAGAT CCGTTCGATA TGGGGATCGT TTTTGGAGGT 
AACAGTGGTG GCGGCAAGCC ACTTTTAGGG CAAGCCTCTG ATATGTCAGA TACGCATAGT 
GGAGGGTCAC AGAGCCCTCT TATAATGGGA GCAGGATGTG GGGATTTTAA GGGGGCAGAA 
CCCAAAGTGA AACAGCAACA GAGTCACATG CGGCCAAAGG ATGAGAATGG GGGTAGCAGT 
GCGGGTAATG CTGTGGTAGG GATGGGGAGT AGTTCAACAG AGTGCAAACA AATCAAACGA 
AGCAGGACCC CATCCAGTGA GGGGAAGTGC AAAGAAAAGC CTCCCAAACG TAAAAAGGTG 
GACTCGGATG GGAAATCGCC CTCTCACAGT TCTGGGGGAC GACCATATAC GCCCCCTAGT 
GGTGGCTCTG GGTCTGGAGG AAGCATGAGT GGGGGAGGCT CTAAATCTCC CGGTAGTTCA 
GGGCGCTCAC AAACGCCCCC TGGTGGTGCC ACGCCGCCGA TTCCCAAAAT AACTATTCAA 
ATAACAAAAG GGACTATAAC TGGGGGGAAA ACATCATCTC TTAGTGGATA CACCTCCAGC 
AGCTCAGCCA CAGGTGGCAC AGGAGCAACA GGGGGAAACA GTGGCAGCAA AAGTCATCAT 
TCGCACTCCT CCTCCTCCTC TGGGAAGATC AAGTCCAAGG AAAGTTCTGC TACACCAGGC 
AGCGCGTCAA AGCCGGGCAG TGCGGGAATA ACAAGTGGAG TGCCATCCCA GTCTAAAACT 
CCTTCACAAG GAATAGGCAT TGGTAAACCT GGATCCTCCC CAATTAATAA ACATGGTTTG 
TCGGGGCCTG GAAGCAGCGG CAGTGGAATT GTGGGAGGCA ATAAGACAAA ACCTCCAGGG 
GGCAAGCCTC CTGGGTCTCT TATGAATCCC AACATTAAGC CCAACATATC CCCTTCTCAC 
TCCCGCTCCA GTAGCTCTGG TGACAAAATG TCTTCCCCAC TGAAAATGCA GCAAGCCCAA 
GTTCCAGGAA CACCCCCTTC CTCTAAAGCG AAATCACCAA TTGGCTCAGG TGGCAGCAGT 
TCCGCAGGGT CCAAATTATC CTCCGGCCCA GGCATGAGCT CCCAGAAACC GCTGGGTGGG 
GGCTCCTCCT CTGGATCCAC ATCTTCATCA TCGTCTTCCA GCTCAACATC ATCATCCGGC 
TCCATGACTT TTTCAGGAAG CTCGCAGTCT CAGTACGGGG GAGGTGGCAG TGGAGGAGGA 
GGTGGTAGCG CGGCAGGAGG AAGCGGATGT GGAGGCGGTG GTAGCAGTGG AGGAAGCGGA 
GGTGGCACAG TAGCCGGTCA GAACAATGCA AACAACCCCA ACGCCAAGGG AAAATCCCCC 
AGTCGAAACA AGAAACCCTC TCTCACAGCA GTCATAGACA AATTAAAAAG TGTAGGTGGA 
GGAGGAGTTG GGGAGGATGG TTGTGAGGTT GGGCCACCTG TAGGTGGTCC TGGTTCTGGA 
AGTGTTCCTA GTGGTGGTGG ACCTGGAAAT GTTCCTAGTG GTGGAGCGCT TAACATATGT 
CCTTCTAAAC ACATCTCAAC CTCTCAAGGT GGTGAGTATA AGAGGGAGAA GTCTGAAAAG 
GAAGCAAAAG CAAAAGTGTC TGTTTCAGGG GGGAACAGCG GGGATAAAAA GCTGATGGAC 
CCCAAAACGG GAGGTGTGGG TACCACTGGT TTGGCTAAAA TTATCATTAG CAAACCTGAT 
GGTGGCTCAC CAAGCATTAA GGCCAAAGGG CCTCTCCAGA AATCAGGAGA AGGGTCCGGT 
GACTCAATTC GCCCCCAGAT GACTAGCCTC AAAGCTTCCC CCCTTTTCAG TGGCTCAACC 
CCCAAGCACG ACCGCTCCTC TCCGAGCCAC AGCCGCTCAC CTGGATGCAC CCCGCTCAAC 
CACGACAGCG AGAGCGAGTC GGGCAGCAGC TCGGTGGCCG AGAAATCCCA CCAGAACAGT 
CCCAGCTCAG ACGACGACCA GACTATCCGA CCGCTTCCAC CTCAGGACTA CTTGAGCTCT 
ATTCCTCTCA GCTCAGGGGA GAAGCACAAG AAACACAAGA AGGAAAAGAA AAAGAAAGAG 
CGGGAACGGG AAAGAGACAG AGATCGGGAG AGGGACCGGG ACAAAGAGAA AAAGAAATCA 
TCCATGTCCA TGGTCCCGTC CTCGCATCCC ATCAAAGCAG ACAGTTGGTC CCGGTCACCC 
ATCTCTGCTT CAGATTCTTC TTTGTCCATG CTGGGTCCAG AGCGTCCCAC CCGGCCCAGC 
CCATTGTATA TGAGAAATGA AGACGATGAC CTCATGGACT CAGCTCTGAC TGGCAACATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011620170.1
CDS	79..5301
Translation	MHSCLENLQRAMKVSSLSAMTDRLESIARQNMLGSHLSPSGTECYITSDMFYVEVQLDNGGQLVDVKVAHQGENPTSCLELIQHLREKNFDEFSKHLKGLVELYKLPGDNKLKTKMYIALQSLELDLTKMMHMYRLATSANTLETLLHGSVGLLSARSGGHLVSLHCYLSPYDLLEERTCAPLNLTDNNVPRSLGVSVSVTIEGTSAVYKLPIAPLITGSHPVDNKGTPSFSTVTNSNCVDLPACFFLKLNRPMPFSVAFIQRMGNATSIPVFESPPTLSPLYQLIVQSELRLQEDGSVTPPCPHNMTFYSVLPGQLHCYFLNGDAPVQDGQSLQGAIVSKIPFRHPAQVPLLLDIVRHQAAYNSLISSCVKRTSVDEDGAGLLQFEVCPLTDSSFSVSFQHPVNESLVCVVMDVIDSRQVSCKLYKGPSDALICTDDFITKVVHRCMSIPVTMRAIRRKAETIQADTPALSLIAQTVEIMVKNNLPPSGSPTYNMASCDGPNPMGLPGLTGGNTPTGGGPPGGPNFAGPITSLFGISRMERQAQGGECPSQPGGTGQQQLQQHQQQQAGQGHSEDYNKVTQNPILTSLLQITGSVGSSPNSQNAPTPHQTPPPTSSPASNTKNHPMLMNLLKDNPAQDFAALYGSSPLERQNSSSGSPRTEGMAGTCPGGSQKVKKKRPRVVEKGSMLPGASASGGSGLSMKSQQSSSMSQQHQHHPVSHEDDFHRELLSMDVDASQNSIFDVNLTGDGLDTPHSITPAPSQCGTPPAGPNMPYSQSHVQSQQQQPQGAAPSHMVRLSSSDSIGPDITEILSDLPEQTGKGSSGSHGQHPIGSSGEEGGPLGTPIRDSSSSGQGSAVFDSADIFNTNSNENPFTDAADLIAEAAATAATPTSDSSSNHFFPDAADFNADLLTSGHGFSQNYFDDSSPSADGDMDLVKGFGGSSQQNTPSGTPQNPTSHGQSTPEPSLKDPFDMGIVFGGNSGGGKPLLGQASDMSDTHSGGSQSPLIMGAGCGDFKGAEPKVKQQQSHMRPKDENGGSSAGNAVVGMGSSSTECKQIKRSRTPSSEGKCKEKPPKRKKVDSDGKSPSHSSGGRPYTPPSGGSGSGGSMSGGGSKSPGSSGRSQTPPGGATPPIPKITIQITKGTITGGKTSSLSGYTSSSSATGGTGATGGNSGSKSHHSHSSSSSGKIKSKESSATPGSASKPGSAGITSGVPSQSKTPSQGIGIGKPGSSPINKHGLSGPGSSGSGIVGGNKTKPPGGKPPGSLMNPNIKPNISPSHSRSSSSGDKMSSPLKMQQAQVPGTPPSSKAKSPIGSGGSSSAGSKLSSGPGMSSQKPLGGGSSSGSTSSSSSSSSTSSSGSMTFSGSSQSQYGGGGSGGGGGSAAGGSGCGGGGSSGGSGGGTVAGQNNANNPNAKGKSPSRNKKPSLTAVIDKLKSVGGGGVGEDGCEVGPPVGGPGSGSVPSGGGPGNVPSGGALNICPSKHISTSQGGEYKREKSEKEAKAKVSVSGGNSGDKKLMDPKTGGVGTTGLAKIIISKPDGGSPSIKAKGPLQKSGEGSGDSIRPQMTSLKASPLFSGSTPKHDRSSPSHSRSPGCTPLNHDSESESGSSSVAEKSHQNSPSSDDDQTIRPLPPQDYLSSIPLSSGEKHKKHKKEKKKKERERERDRDRERDRDKEKKKSSMSMVPSSHPIKADSWSRSPISASDSSLSMLGPERPTRPSPLYMRNEDDDLMDSALTGNI

Target ORF information:

RefSeq Version	XM_011621868.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mediator complex subunit 1 (med1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011621868.1

ORF Insert Sequence:

ATGCACAGCT GCTTAGAGAA CTTGCAGAGA GCGATGAAAG TTTCATCTTT GTCTGCCATG 
ACAGACCGTT TAGAGTCCAT CGCTCGGCAA AACATGCTGG GCTCTCACCT CAGCCCGTCA 
GGGACAGAAT GTTACATCAC GTCAGACATG TTTTATGTGG AGGTGCAGCT GGACAACGGT 
GGTCAGCTAG TAGATGTCAA GGTGGCTCAT CAGGGAGAAA ACCCTACAAG TTGCCTGGAG 
CTCATTCAGC ATCTTCGGGA GAAGAACTTT GATGAGTTTT CAAAACATCT GAAGGGCCTG 
GTTGAACTAT ACAAGCTCCC CGGTGATAAT AAGTTAAAAA CCAAGATGTA CATTGCTCTA 
CAGTCTTTGG AGCTTGATCT CACCAAGATG ATGCACATGT ACAGGTTAGC AACCAGTGCT 
AATACACTGG AGACTCTTCT TCATGGCAGC GTAGGCTTGT TGTCTGCAAG GAGCGGTGGT 
CATCTTGTTT CTCTTCACTG TTACCTCTCA CCATATGATT TACTGGAGGA AAGAACCTGT 
GCGCCACTCA ATTTAACAGA CAACAATGTT CCGCGTTCTC TGGGTGTGAG CGTTTCTGTG 
ACAATTGAGG GAACCTCTGC TGTTTACAAA CTCCCGATCG CTCCACTCAT CACTGGATCA 
CATCCCGTGG ATAACAAGGG GACACCTTCA TTTTCCACTG TGACTAATTC CAACTGTGTG 
GACCTGCCAG CTTGTTTTTT CCTCAAGCTG AATCGTCCCA TGCCTTTCTC AGTGGCCTTC 
ATTCAGAGGA TGGGCAATGC TACATCTATT CCAGTTTTTG AGAGCCCACC CACTCTCTCG 
CCTCTTTACC AATTAATTGT CCAGAGTGAG CTTCGCCTCC AAGAAGATGG GAGTGTCACA 
CCACCGTGTC CACACAACAT GACCTTCTAC TCAGTATTGC CAGGGCAGCT GCACTGTTAT 
TTTTTGAATG GTGACGCCCC AGTGCAAGAT GGTCAGTCTC TTCAAGGGGC AATCGTGTCC 
AAGATCCCCT TCCGCCACCC AGCCCAGGTC CCACTTCTGT TAGATATCGT TCGGCACCAG 
GCAGCTTATA ATTCCTTGAT TAGCAGCTGT GTGAAACGCA CTTCAGTCGA CGAAGATGGC 
GCAGGCCTGC TGCAGTTTGA AGTGTGTCCC CTGACTGACT CCAGTTTTAG TGTCTCGTTC 
CAGCATCCAG TCAATGAGTC GTTGGTTTGC GTTGTCATGG ATGTGATCGA CTCCAGACAG 
GTCTCCTGCA AGTTATATAA AGGACCTTCG GATGCATTAA TTTGTACTGA TGATTTCATC 
ACCAAAGTTG TTCACAGATG CATGTCCATC CCAGTAACAA TGCGGGCTAT TCGGAGAAAA 
GCGGAGACCA TCCAGGCAGA CACTCCCGCC CTGTCACTTA TTGCACAGAC GGTGGAGATC 
ATGGTGAAGA ACAACCTCCC ACCCTCGGGA AGTCCTACAT ATAACATGGC GTCATGTGAT 
GGACCAAACC CGATGGGACT GCCTGGGCTC ACTGGGGGCA ATACGCCCAC TGGAGGTGGA 
CCACCTGGAG GACCCAATTT TGCTGGCCCA ATTACTTCTC TGTTTGGAAT ATCCCGGATG 
GAAAGGCAAG CTCAAGGAGG AGAGTGTCCC TCACAACCCG GGGGAACTGG CCAGCAACAA 
TTGCAACAGC ATCAGCAACA GCAGGCAGGT CAAGGCCATT CAGAAGACTA CAACAAAGTC 
ACACAGAATC CCATATTGAC AAGTTTGCTG CAGATAACCG GAAGTGTTGG CTCTAGTCCC 
AACTCCCAAA ATGCACCAAC ACCCCACCAG ACCCCACCTC CAACATCGTC CCCTGCGAGC 
AACACGAAAA ATCATCCAAT GTTGATGAAT TTGTTGAAGG ACAACCCTGC GCAAGATTTT 
GCCGCACTGT ATGGTTCTAG TCCATTAGAG CGGCAGAATT CTTCATCCGG ATCTCCACGG 
ACGGAGGGCA TGGCTGGAAC ATGCCCTGGA GGTAGCCAAA AGGTTAAAAA GAAACGTCCA 
AGGGTGGTGG AAAAGGGCAG CATGTTGCCC GGTGCTTCTG CTAGTGGTGG TAGCGGTTTA 
AGCATGAAGT CCCAACAGTC TTCCTCGATG TCACAGCAGC ACCAACACCA CCCTGTCTCG 
CATGAAGACG ATTTCCACCG TGAGCTTCTC TCTATGGATG TAGATGCTTC TCAGAATTCG 
ATATTTGACG TTAACCTCAC CGGTGATGGC CTCGACACAC CACATAGCAT CACTCCAGCA 
CCCAGCCAAT GTGGAACGCC ACCAGCTGGC CCCAACATGC CGTACTCGCA GTCCCACGTC 
CAATCCCAGC AACAGCAACC CCAAGGTGCA GCACCGTCCC ATATGGTCCG CTTGTCTAGT 
TCTGACAGCA TTGGGCCTGA TATCACAGAA ATTTTGTCAG ACCTGCCCGA GCAGACAGGA 
AAAGGCAGCA GTGGAAGCCA TGGACAGCAC CCCATTGGCA GCAGCGGAGA GGAAGGAGGC 
CCTCTCGGTA CCCCGATACG TGACTCCTCG AGCTCAGGTC AGGGTAGTGC AGTTTTTGAC 
TCGGCGGATA TTTTCAACAC GAACAGCAAC GAGAATCCTT TTACCGATGC CGCTGACTTG 
ATCGCAGAGG CGGCCGCAAC TGCTGCTACT CCCACCAGTG ATTCCTCTTC GAACCATTTC 
TTCCCCGACG CTGCCGACTT CAATGCTGAC CTCCTGACTT CAGGCCATGG CTTCTCGCAG 
AACTACTTTG ATGACAGCTC GCCGAGTGCA GACGGAGATA TGGATCTGGT CAAAGGGTTT 
GGGGGGAGCA GCCAGCAAAA CACTCCATCA GGAACGCCTC AGAATCCAAC GTCACATGGG 
CAAAGCACCC CAGAGCCGTC GCTGAAAGAT CCGTTCGATA TGGGGATCGT TTTTGGAGGT 
AACAGTGGTG GCGGCAAGCC ACTTTTAGGG CAAGCCTCTG ATATGTCAGA TACGCATAGT 
GGAGGGTCAC AGAGCCCTCT TATAATGGGA GCAGGATGTG GGGATTTTAA GGGGGCAGAA 
CCCAAAGTGA AACAGCAACA GAGTCACATG CGGCCAAAGG ATGAGAATGG GGGTAGCAGT 
GCGGGTAATG CTGTGGTAGG GATGGGGAGT AGTTCAACAG AGTGCAAACA AATCAAACGA 
AGCAGGACCC CATCCAGTGA GGGGAAGTGC AAAGAAAAGC CTCCCAAACG TAAAAAGGTG 
GACTCGGATG GGAAATCGCC CTCTCACAGT TCTGGGGGAC GACCATATAC GCCCCCTAGT 
GGTGGCTCTG GGTCTGGAGG AAGCATGAGT GGGGGAGGCT CTAAATCTCC CGGTAGTTCA 
GGGCGCTCAC AAACGCCCCC TGGTGGTGCC ACGCCGCCGA TTCCCAAAAT AACTATTCAA 
ATAACAAAAG GGACTATAAC TGGGGGGAAA ACATCATCTC TTAGTGGATA CACCTCCAGC 
AGCTCAGCCA CAGGTGGCAC AGGAGCAACA GGGGGAAACA GTGGCAGCAA AAGTCATCAT 
TCGCACTCCT CCTCCTCCTC TGGGAAGATC AAGTCCAAGG AAAGTTCTGC TACACCAGGC 
AGCGCGTCAA AGCCGGGCAG TGCGGGAATA ACAAGTGGAG TGCCATCCCA GTCTAAAACT 
CCTTCACAAG GAATAGGCAT TGGTAAACCT GGATCCTCCC CAATTAATAA ACATGGTTTG 
TCGGGGCCTG GAAGCAGCGG CAGTGGAATT GTGGGAGGCA ATAAGACAAA ACCTCCAGGG 
GGCAAGCCTC CTGGGTCTCT TATGAATCCC AACATTAAGC CCAACATATC CCCTTCTCAC 
TCCCGCTCCA GTAGCTCTGG TGACAAAATG TCTTCCCCAC TGAAAATGCA GCAAGCCCAA 
GTTCCAGGAA CACCCCCTTC CTCTAAAGCG AAATCACCAA TTGGCTCAGG TGGCAGCAGT 
TCCGCAGGGT CCAAATTATC CTCCGGCCCA GGCATGAGCT CCCAGAAACC GCTGGGTGGG 
GGCTCCTCCT CTGGATCCAC ATCTTCATCA TCGTCTTCCA GCTCAACATC ATCATCCGGC 
TCCATGACTT TTTCAGGAAG CTCGCAGTCT CAGTACGGGG GAGGTGGCAG TGGAGGAGGA 
GGTGGTAGCG CGGCAGGAGG AAGCGGATGT GGAGGCGGTG GTAGCAGTGG AGGAAGCGGA 
GGTGGCACAG TAGCCGGTCA GAACAATGCA AACAACCCCA ACGCCAAGGG AAAATCCCCC 
AGTCGAAACA AGAAACCCTC TCTCACAGCA GTCATAGACA AATTAAAAAG TGTAGGTGGA 
GGAGGAGTTG GGGAGGATGG TTGTGAGGTT GGGCCACCTG TAGGTGGTCC TGGTTCTGGA 
AGTGTTCCTA GTGGTGGTGG ACCTGGAAAT GTTCCTAGTG GTGGAGCGCT TAACATATGT 
CCTTCTAAAC ACATCTCAAC CTCTCAAGGT GGTGAGTATA AGAGGGAGAA GTCTGAAAAG 
GAAGCAAAAG CAAAAGTGTC TGTTTCAGGG GGGAACAGCG GGGATAAAAA GCTGATGGAC 
CCCAAAACGG GAGGTGTGGG TACCACTGGT TTGGCTAAAA TTATCATTAG CAAACCTGAT 
GGTGGCTCAC CAAGCATTAA GGCCAAAGGG CCTCTCCAGA AATCAGGAGA AGGGTCCGGT 
GACTCAATTC GCCCCCAGAT GACTAGCCTC AAAGCTTCCC CCCTTTTCAG TGGCTCAACC 
CCCAAGCACG ACCGCTCCTC TCCGAGCCAC AGCCGCTCAC CTGGATGCAC CCCGCTCAAC 
CACGACAGCG AGAGCGAGTC GGGCAGCAGC TCGGTGGCCG AGAAATCCCA CCAGAACAGT 
CCCAGCTCAG ACGACGACCA GACTATCCGA CCGCTTCCAC CTCAGGACTA CTTGAGCTCT 
ATTCCTCTCA GCTCAGGGGA GAAGCACAAG AAACACAAGA AGGAAAAGAA AAAGAAAGAG 
CGGGAACGGG AAAGAGACAG AGATCGGGAG AGGGACCGGG ACAAAGAGAA AAAGAAATCA 
TCCATGTCCA TGGTCCCGTC CTCGCATCCC ATCAAAGCAG ACAGTTGGTC CCGGTCACCC 
ATCTCTGCTT CAGATTCTTC TTTGTCCATG CTGGGTCCAG AGCGTCCCAC CCGGCCCAGC 
CCATTGTATA TGAGAAATGA AGACGATGAC CTCATGGACT CAGCTCTGAC TGGCAACATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med1 ( XM_003961249.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003961298.2 Takifugu rubripes mediator complex subunit 1 (med1), mRNA.

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CloneID	OTo42077
Clone ID Related Accession (Same CDS sequence)	XM_003961249.3
Accession Version	XM_003961249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	mediator of RNA polymerase II transcription subunit 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003961249.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGCCTGGTGT TGCTCTGCAG TGTGATAGCC CTGCAAGAGA GCTCTCACTT 
TCCGTTCATC AAGGAAGTAA TCAGATTCAT GGAGATCGAA TCAAAACTGA AGAGTCAGCA 
GAGGAGGAGA AGGAAAGTCG CTCAGCTGTT TTGCTGGAGA AGCTTCACGC CAAGTATATT 
GCTTCAAGGC CATGGCAGGA GACCTTCAAA CTAGTGCGTC AGGCCATGGA CAAACGAGGT 
CCAACAAATG CAGCAAGTCA CCAGTTGATG CACAGCTGCT TAGAGAACTT GCAGAGAGCG 
ATGAAAGTTT CATCTTTGTC TGCCATGACA GACCGTTTAG AGTCCATCGC TCGGCAAAAC 
ATGCTGGGCT CTCACCTCAG CCCGTCAGGG ACAGAATGTT ACATCACGTC AGACATGTTT 
TATGTGGAGG TGCAGCTGGA CAACGGTGGT CAGCTAGTAG ATGTCAAGGT GGCTCATCAG 
GGAGAAAACC CTACAAGTTG CCTGGAGCTC ATTCAGCATC TTCGGGAGAA GAACTTTGAT 
GAGTTTTCAA AACATCTGAA GGGCCTGGTT GAACTATACA AGCTCCCCGG TGATAATAAG 
TTAAAAACCA AGATGTACAT TGCTCTACAG TCTTTGGAGC TTGATCTCAC CAAGATGATG 
CACATGTACA GGTTAGCAAC CAGTGCTAAT ACACTGGAGA CTCTTCTTCA TGGCAGCGTA 
GGCTTGTTGT CTGCAAGGAG CGGTGGTCAT CTTGTTTCTC TTCACTGTTA CCTCTCACCA 
TATGACTTAC TGGAGGAAAG AACCTGTGCG CCACTCAATT TAACAGACAA CAATGTTCCG 
CGTTCTCTGG GTGTGAGCGT TTCTGTGACA ATTGAGGGAA CCTCTGCTGT TTACAAACTC 
CCGATCGCTC CACTCATCAC TGGATCACAT CCCGTGGATA ACAAGGGGAC ACCTTCATTT 
TCCACTGTGA CTAATTCCAA CTGTGTGGAC CTGCCAGCTT GTTTTTTCCT CAAGCTGAAT 
CGTCCCATGC CTTTCTCAGT GGCCTTCATT CAGAGGATGG GCAATGCTAC ATCTATTCCA 
GTTTTTGAGA GCCCACCCAC TCTCTCGCCT CTTTACCAAT TAATTGTCCA GAGTGAGCTT 
CGCCTCCAAG AAGATGGGAG TGTCACACCA CCGTGTCCAC ACAACATGAC CTTCTACTCA 
GTATTGCCAG GGCAGCTGCA CTGTTATTTT TTGAATGGTG ACGCCCCAGT GCAAGATGGT 
CAGTCTCTTC AAGGGGCAAT CGTGTCCAAG ATCCCCTTCC GCCACCCAGC CCAGGTCCCA 
CTTCTGTTAG ATATCGTTCG GCACCAGGCA GCTTATAATT CCTTGATTAG CAGCTGTGTG 
AAACGCACTT CAGTCGACGA AGATGGCGCA GGCCTGCTGC AGTTTGAAGT GTGTCCCCTG 
ACTGACTCCA GTTTTAGTGT CTCGTTCCAG CATCCAGTCA ATGAGTCGTT GGTTTGCGTT 
GTCATGGATG TGATCGACTC CAGACAGGTC TCCTGCAAGT TATATAAAGG ACCTTCGGAT 
GCATTAATTT GTACTGATGA TTTCATCACC AAAGTTGTTC ACAGATGCAT GTCCATCCCA 
GTAACAATGC GGGCTATTCG GAGAAAAGCG GAGACCATCC AGGCAGACAC TCCCGCCCTG 
TCACTTATCG CACAGACGGT GGAGATCATG GTGAAGAACA ACCTCCCACC CTCGGGAAGT 
CCTACATATA ACATGGCGTC ATGTGATGGA CCAAACCCGA TGGGACTGCC TGGGCTCACT 
GGGGGCAATA CGCCCACTGG AGGTGGACCA CCTGGAGGAC CCAATTTTGC TGGCCCAATT 
ACTTCTCTGT TTGGAATATC CCGGATGGAA AGGCAAGCTC AAGGAGGAGA GTGTCCCTCA 
CAACCCGGGG GAACTGGCCA GCAACAATTG CAACAGCATC AGCAACAGCA GGCAGGTCAA 
GGCCATTCAG AAGACTACAA CAAAGTCACA CAGAATCCCA TATTGACAAG TTTGCTGCAG 
ATAACCGGAA GTGTTGGCTC TAGTCCCAAC TCCCAAAATG CACCAACACC CCACCAGACC 
CCACCTCCAA CATCGTCTCC TGCGAGCAAC ACGAAAAATC ATCCAATGTT GATGAATTTG 
TTGAAGGACA ACCCTGCGCA AGATTTTGCC GCACTGTATG GTTCTAGTCC ATTAGAGCGG 
CAGAATTCTT CATCCGGATC TCCACGGACG GAGGGCATGG CTGGAACATG CCCTGGAGGT 
AGCCAAAAGG TTAAAAAGAA ACGTCCAAGG GTGGTGGAAA AGGGCAGCAT GTTGCCCGGT 
GCTTCTGCTA GTGGTGGTAG CGGTTTAAGC ATGAAGTCCC AACAGTCTTC CTCGATGTCA 
CAGCAGCACC AACACCACCC TGTCTCGCAT GAAGACGATT TCCACCGTGA GCTTCTCTCT 
ATGGATGTAG ATGCTTCTCA GAATTCGATA TTTGACGTTA ACCTCACCGG TGATGGCCTC 
GACACACCAC ATAGCATCAC TCCAGCACCC AGCCAATGTG GAACGCCACC AGCTGGCCCC 
AACATGCCGT ACTCGCAGTC CCACGTCCAA TCCCAGCAAC AGCAACCCCA AGGTGCAGCA 
CCGTCCCATA TGGTCCGCTT GTCTAGTTCT GACAGCATTG GGCCTGATAT CACAGAAATT 
TTGTCAGACC TGCCCGAGCA GACAGGAAAA GGCAGCAGTG GAAGCCATGG ACAGCACCCC 
ATTGGCAGCA GCGGAGAGGA AGGAGGCCCT CTCGGTACCC CGATACGTGA CTCCTCGAGC 
TCAGGTCAGG GTAGTGCAGT TTTTGACTCG GCGGATATTT TCAACACGAA CAGCAACGAG 
AATCCTTTTA CCGATGCCGC TGACTTGATC GCAGAGGCGG CCGCAACTGC TGCTACTCCC 
ACCAGTGATT CCTCTTCGAA CCATTTCTTC CCCGACGCTG CCGACTTCAA TGCTGACCTC 
CTGACTTCAG GCCATGGCTT CTCGCAGAAC TACTTTGATG ACAGCTCGCC GAGTGCAGAC 
GGAGATATGG ATCTGGTCAA AGGGTTTGGG GGGAGCAGCC AGCAAAACAC TCCATCAGGA 
ACGCCTCAGA ATCCAACGTC ACATGGGCAA AGCACCCCAG AGCCGTCGCT GAAAGATCCG 
TTCGATATGG GGATCGTTTT TGGAGGTAAC AGTGGTGGCG GCAAGCCACT TTTAGGGCAA 
GCCTCTGATA TGTCAGATAC GCATAGTGGA GGGTCACAGA GCCCTCTTAT AATGGGAGCA 
GGATGTGGGG ATTTTAAGGG GGCAGAACCC AAAGTGAAAC AGCAACAGAG TCACATGCGG 
CCAAAGGATG AGAATGGGGG TAGCAGTGCG GGTAATGCTG TGGTAGGGAT GGGGAGTAGT 
TCAACAGAGT GCAAACAAAT CAAACGAAGC AGGACCCCAT CCAGTGAGGG GAAGTGCAAA 
GAAAAGCCTC CCAAACGTAA AAAGGTGGAC TCGGATGGGA AATCGCCCTC TCACAGTTCT 
GGGGGACGAC CATATACGCC CCCTAGTGGT GGCTCTGGGT CTGGAGGAAG CATGAGTGGG 
GGAGGCTCTA AATCTCCCGG TAGTTCAGGG CGCTCACAAA CGCCCCCTGG TGGTGCCACA 
CCGCCGATTC CCAAAATAAC TATTCAAATA ACAAAAGGGA CTATAACTGG GGGGAAAACA 
TCATCTCTTA GTGGATACAC CTCCAGCAGC TCAGCCACAG GTGGCACAGG AGCAACAGGG 
GGAAACAGTG GCAGCAAAAG TCATCATTCG CACTCCTCCT CCTCCTCTGG GAAGATCAAG 
TCCAAGGAAA GTTCTGCTAC ACCAGGCAGC GCGTCAAAGC CGGGCAGTGC GGGAATAACA 
AGTGGAGTGC CATCCCAGTC TAAAACTCCT TCACAAGGAA TAGGCATTGG TAAACCTGGA 
TCCTCCCCAA TTAATAAACA TGGTTTGTCG GGGCCTGGAA GCAGCGGCAG TGGAATTGTG 
GGAGGCAATA AGACAAAACC TCCAGGGGGC AAGCCTCCTG GGTCTCTTAT GAATCCCAAC 
ATTAAGCCCA ACATATCCCC TTCTCACTCC CGCTCCAGTA GCTCTGGTGA CAAAATGTCT 
TCCCCACTGA AAATGCAGCA AGCCCAAGTT CCAGGAACAC CCCCTTCCTC TAAAGCGAAA 
TCACCAATTG GCTCAGGTGG CAGCAGTTCC GCAGGGTCCA AATTATCCTC CGGCCCAGGC 
ATGAGCTCCC AGAAACCGCT GGGTGGGAGC TCCTCCTCTG GATCCACATC TTCATCATCG 
TCTTCCAGCT CAACATCATC ATCCGGCTCC ATGACTTTTT CAGGAAGCTC GCAGTCTCAG 
TACGGGGGAG GTGGCAGTGG AGGAGGAGGT GGTAGCGCGG CAGGAGGAAG CGGATGTGGA 
GGCGGTGGTA GCAGTGGAGG AAGCGGAGGT GGCACAGTAG CCGGTCAGAA CAATGCAAAC 
AACCCCAACG CCAAGGGAAA ATCCCCCAGT CGAAACAAGA AACCCTCTCT CACAGCAGTC 
ATAGACAAAT TAAAAAGTGT AGGTGGAGGA GGAGTTGGGG AGGATGGTTG TGAGGTTGGG 
CCACCTGTAG GTGGTCCTGG TTCTGGAAGT GTTCCTAGTG GTGGTGGACC TGGAAATGTT 
CCTAGTGGTG GAGCGCTTAA CATTTGTCCT TCTAAACACA TCTCAACCTC TCAAGGTGGT 
GAGTATAAGA GGGAGAAGTC TGAAAAGGAA GCAAAAGCGA AAGTGTCTGT TTCAGGGGGG 
AACAGCGGGG ATAAAAAGCT GATGGACCCC AAAACGGGAG GTGTGGGTAC CACTGGTTTG 
GCTAAAATTA TCATTAGCAA ACCTGATGGT GGCTCACCAA GCATTAAGGC CAAAGGGCCT 
CTCCAGAAAT CAGGAGAAGG GTCCGGTGAC TCAATTCGCC CCCAGATGAC TAGCCTCAAA 
GCTTCCCCCC TTTTCAGTGG CTCAACCCCC AAGCACGACC GCTCCTCCCC GAGCCACAGC 
CGCTCACCTG GATGCACCCC GCTCAACCAC GACAGCGAGA GCGAGTCGGG CAGCAGCTCG 
GTGGCCGAGA AATCCCACCA GAACAGTCCC AGCTCAGACG ACGACCAGAC TATCCGACCG 
CTTCCACCTC AGGACTACTT GAGCTCTATT CCTCTCAGCT CAGGGGAGAA GCACAAGAAA 
CACAAGAAGG AAAAGAAAAA GAAAGAGCGG GAACGGGAAA GAGACAGAGA TCGGGAGAGG 
GACCGGGACA AAGAGAAAAA GAAATCATCC ATGTCCATGG TCCCGTCCTC GCATCCCATC 
AAAGCAGACA GTTGGTCCCG GTCACCCATC TCTGCTTCAG ATTCTTCTTT GTCCATGCTG 
GGTCCAGAGC GTCCCACCCG GCCCAGCCCA TTGTATATGA GAAATGAAGA CGATGACCTC 
ATGGACTCAG CTCTGACTGG CAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961298.2
CDS	205..5694
Translation	MAAGPGVALQCDSPARELSLSVHQGSNQIHGDRIKTEESAEEEKESRSAVLLEKLHAKYIASRPWQETFKLVRQAMDKRGPTNAASHQLMHSCLENLQRAMKVSSLSAMTDRLESIARQNMLGSHLSPSGTECYITSDMFYVEVQLDNGGQLVDVKVAHQGENPTSCLELIQHLREKNFDEFSKHLKGLVELYKLPGDNKLKTKMYIALQSLELDLTKMMHMYRLATSANTLETLLHGSVGLLSARSGGHLVSLHCYLSPYDLLEERTCAPLNLTDNNVPRSLGVSVSVTIEGTSAVYKLPIAPLITGSHPVDNKGTPSFSTVTNSNCVDLPACFFLKLNRPMPFSVAFIQRMGNATSIPVFESPPTLSPLYQLIVQSELRLQEDGSVTPPCPHNMTFYSVLPGQLHCYFLNGDAPVQDGQSLQGAIVSKIPFRHPAQVPLLLDIVRHQAAYNSLISSCVKRTSVDEDGAGLLQFEVCPLTDSSFSVSFQHPVNESLVCVVMDVIDSRQVSCKLYKGPSDALICTDDFITKVVHRCMSIPVTMRAIRRKAETIQADTPALSLIAQTVEIMVKNNLPPSGSPTYNMASCDGPNPMGLPGLTGGNTPTGGGPPGGPNFAGPITSLFGISRMERQAQGGECPSQPGGTGQQQLQQHQQQQAGQGHSEDYNKVTQNPILTSLLQITGSVGSSPNSQNAPTPHQTPPPTSSPASNTKNHPMLMNLLKDNPAQDFAALYGSSPLERQNSSSGSPRTEGMAGTCPGGSQKVKKKRPRVVEKGSMLPGASASGGSGLSMKSQQSSSMSQQHQHHPVSHEDDFHRELLSMDVDASQNSIFDVNLTGDGLDTPHSITPAPSQCGTPPAGPNMPYSQSHVQSQQQQPQGAAPSHMVRLSSSDSIGPDITEILSDLPEQTGKGSSGSHGQHPIGSSGEEGGPLGTPIRDSSSSGQGSAVFDSADIFNTNSNENPFTDAADLIAEAAATAATPTSDSSSNHFFPDAADFNADLLTSGHGFSQNYFDDSSPSADGDMDLVKGFGGSSQQNTPSGTPQNPTSHGQSTPEPSLKDPFDMGIVFGGNSGGGKPLLGQASDMSDTHSGGSQSPLIMGAGCGDFKGAEPKVKQQQSHMRPKDENGGSSAGNAVVGMGSSSTECKQIKRSRTPSSEGKCKEKPPKRKKVDSDGKSPSHSSGGRPYTPPSGGSGSGGSMSGGGSKSPGSSGRSQTPPGGATPPIPKITIQITKGTITGGKTSSLSGYTSSSSATGGTGATGGNSGSKSHHSHSSSSSGKIKSKESSATPGSASKPGSAGITSGVPSQSKTPSQGIGIGKPGSSPINKHGLSGPGSSGSGIVGGNKTKPPGGKPPGSLMNPNIKPNISPSHSRSSSSGDKMSSPLKMQQAQVPGTPPSSKAKSPIGSGGSSSAGSKLSSGPGMSSQKPLGGSSSSGSTSSSSSSSSTSSSGSMTFSGSSQSQYGGGGSGGGGGSAAGGSGCGGGGSSGGSGGGTVAGQNNANNPNAKGKSPSRNKKPSLTAVIDKLKSVGGGGVGEDGCEVGPPVGGPGSGSVPSGGGPGNVPSGGALNICPSKHISTSQGGEYKREKSEKEAKAKVSVSGGNSGDKKLMDPKTGGVGTTGLAKIIISKPDGGSPSIKAKGPLQKSGEGSGDSIRPQMTSLKASPLFSGSTPKHDRSSPSHSRSPGCTPLNHDSESESGSSSVAEKSHQNSPSSDDDQTIRPLPPQDYLSSIPLSSGEKHKKHKKEKKKKERERERDRDRERDRDKEKKKSSMSMVPSSHPIKADSWSRSPISASDSSLSMLGPERPTRPSPLYMRNEDDDLMDSALTGNI

Target ORF information:

RefSeq Version	XM_003961249.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mediator complex subunit 1 (med1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961249.3

ORF Insert Sequence:

ATGGCGGCGG GGCCTGGTGT TGCTCTGCAG TGTGATAGCC CTGCAAGAGA GCTCTCACTT 
TCCGTTCATC AAGGAAGTAA TCAGATTCAT GGAGATCGAA TCAAAACTGA AGAGTCAGCA 
GAGGAGGAGA AGGAAAGTCG CTCAGCTGTT TTGCTGGAGA AGCTTCACGC CAAGTATATT 
GCTTCAAGGC CATGGCAGGA GACCTTCAAA CTAGTGCGTC AGGCCATGGA CAAACGAGGT 
CCAACAAATG CAGCAAGTCA CCAGTTGATG CACAGCTGCT TAGAGAACTT GCAGAGAGCG 
ATGAAAGTTT CATCTTTGTC TGCCATGACA GACCGTTTAG AGTCCATCGC TCGGCAAAAC 
ATGCTGGGCT CTCACCTCAG CCCGTCAGGG ACAGAATGTT ACATCACGTC AGACATGTTT 
TATGTGGAGG TGCAGCTGGA CAACGGTGGT CAGCTAGTAG ATGTCAAGGT GGCTCATCAG 
GGAGAAAACC CTACAAGTTG CCTGGAGCTC ATTCAGCATC TTCGGGAGAA GAACTTTGAT 
GAGTTTTCAA AACATCTGAA GGGCCTGGTT GAACTATACA AGCTCCCCGG TGATAATAAG 
TTAAAAACCA AGATGTACAT TGCTCTACAG TCTTTGGAGC TTGATCTCAC CAAGATGATG 
CACATGTACA GGTTAGCAAC CAGTGCTAAT ACACTGGAGA CTCTTCTTCA TGGCAGCGTA 
GGCTTGTTGT CTGCAAGGAG CGGTGGTCAT CTTGTTTCTC TTCACTGTTA CCTCTCACCA 
TATGACTTAC TGGAGGAAAG AACCTGTGCG CCACTCAATT TAACAGACAA CAATGTTCCG 
CGTTCTCTGG GTGTGAGCGT TTCTGTGACA ATTGAGGGAA CCTCTGCTGT TTACAAACTC 
CCGATCGCTC CACTCATCAC TGGATCACAT CCCGTGGATA ACAAGGGGAC ACCTTCATTT 
TCCACTGTGA CTAATTCCAA CTGTGTGGAC CTGCCAGCTT GTTTTTTCCT CAAGCTGAAT 
CGTCCCATGC CTTTCTCAGT GGCCTTCATT CAGAGGATGG GCAATGCTAC ATCTATTCCA 
GTTTTTGAGA GCCCACCCAC TCTCTCGCCT CTTTACCAAT TAATTGTCCA GAGTGAGCTT 
CGCCTCCAAG AAGATGGGAG TGTCACACCA CCGTGTCCAC ACAACATGAC CTTCTACTCA 
GTATTGCCAG GGCAGCTGCA CTGTTATTTT TTGAATGGTG ACGCCCCAGT GCAAGATGGT 
CAGTCTCTTC AAGGGGCAAT CGTGTCCAAG ATCCCCTTCC GCCACCCAGC CCAGGTCCCA 
CTTCTGTTAG ATATCGTTCG GCACCAGGCA GCTTATAATT CCTTGATTAG CAGCTGTGTG 
AAACGCACTT CAGTCGACGA AGATGGCGCA GGCCTGCTGC AGTTTGAAGT GTGTCCCCTG 
ACTGACTCCA GTTTTAGTGT CTCGTTCCAG CATCCAGTCA ATGAGTCGTT GGTTTGCGTT 
GTCATGGATG TGATCGACTC CAGACAGGTC TCCTGCAAGT TATATAAAGG ACCTTCGGAT 
GCATTAATTT GTACTGATGA TTTCATCACC AAAGTTGTTC ACAGATGCAT GTCCATCCCA 
GTAACAATGC GGGCTATTCG GAGAAAAGCG GAGACCATCC AGGCAGACAC TCCCGCCCTG 
TCACTTATCG CACAGACGGT GGAGATCATG GTGAAGAACA ACCTCCCACC CTCGGGAAGT 
CCTACATATA ACATGGCGTC ATGTGATGGA CCAAACCCGA TGGGACTGCC TGGGCTCACT 
GGGGGCAATA CGCCCACTGG AGGTGGACCA CCTGGAGGAC CCAATTTTGC TGGCCCAATT 
ACTTCTCTGT TTGGAATATC CCGGATGGAA AGGCAAGCTC AAGGAGGAGA GTGTCCCTCA 
CAACCCGGGG GAACTGGCCA GCAACAATTG CAACAGCATC AGCAACAGCA GGCAGGTCAA 
GGCCATTCAG AAGACTACAA CAAAGTCACA CAGAATCCCA TATTGACAAG TTTGCTGCAG 
ATAACCGGAA GTGTTGGCTC TAGTCCCAAC TCCCAAAATG CACCAACACC CCACCAGACC 
CCACCTCCAA CATCGTCTCC TGCGAGCAAC ACGAAAAATC ATCCAATGTT GATGAATTTG 
TTGAAGGACA ACCCTGCGCA AGATTTTGCC GCACTGTATG GTTCTAGTCC ATTAGAGCGG 
CAGAATTCTT CATCCGGATC TCCACGGACG GAGGGCATGG CTGGAACATG CCCTGGAGGT 
AGCCAAAAGG TTAAAAAGAA ACGTCCAAGG GTGGTGGAAA AGGGCAGCAT GTTGCCCGGT 
GCTTCTGCTA GTGGTGGTAG CGGTTTAAGC ATGAAGTCCC AACAGTCTTC CTCGATGTCA 
CAGCAGCACC AACACCACCC TGTCTCGCAT GAAGACGATT TCCACCGTGA GCTTCTCTCT 
ATGGATGTAG ATGCTTCTCA GAATTCGATA TTTGACGTTA ACCTCACCGG TGATGGCCTC 
GACACACCAC ATAGCATCAC TCCAGCACCC AGCCAATGTG GAACGCCACC AGCTGGCCCC 
AACATGCCGT ACTCGCAGTC CCACGTCCAA TCCCAGCAAC AGCAACCCCA AGGTGCAGCA 
CCGTCCCATA TGGTCCGCTT GTCTAGTTCT GACAGCATTG GGCCTGATAT CACAGAAATT 
TTGTCAGACC TGCCCGAGCA GACAGGAAAA GGCAGCAGTG GAAGCCATGG ACAGCACCCC 
ATTGGCAGCA GCGGAGAGGA AGGAGGCCCT CTCGGTACCC CGATACGTGA CTCCTCGAGC 
TCAGGTCAGG GTAGTGCAGT TTTTGACTCG GCGGATATTT TCAACACGAA CAGCAACGAG 
AATCCTTTTA CCGATGCCGC TGACTTGATC GCAGAGGCGG CCGCAACTGC TGCTACTCCC 
ACCAGTGATT CCTCTTCGAA CCATTTCTTC CCCGACGCTG CCGACTTCAA TGCTGACCTC 
CTGACTTCAG GCCATGGCTT CTCGCAGAAC TACTTTGATG ACAGCTCGCC GAGTGCAGAC 
GGAGATATGG ATCTGGTCAA AGGGTTTGGG GGGAGCAGCC AGCAAAACAC TCCATCAGGA 
ACGCCTCAGA ATCCAACGTC ACATGGGCAA AGCACCCCAG AGCCGTCGCT GAAAGATCCG 
TTCGATATGG GGATCGTTTT TGGAGGTAAC AGTGGTGGCG GCAAGCCACT TTTAGGGCAA 
GCCTCTGATA TGTCAGATAC GCATAGTGGA GGGTCACAGA GCCCTCTTAT AATGGGAGCA 
GGATGTGGGG ATTTTAAGGG GGCAGAACCC AAAGTGAAAC AGCAACAGAG TCACATGCGG 
CCAAAGGATG AGAATGGGGG TAGCAGTGCG GGTAATGCTG TGGTAGGGAT GGGGAGTAGT 
TCAACAGAGT GCAAACAAAT CAAACGAAGC AGGACCCCAT CCAGTGAGGG GAAGTGCAAA 
GAAAAGCCTC CCAAACGTAA AAAGGTGGAC TCGGATGGGA AATCGCCCTC TCACAGTTCT 
GGGGGACGAC CATATACGCC CCCTAGTGGT GGCTCTGGGT CTGGAGGAAG CATGAGTGGG 
GGAGGCTCTA AATCTCCCGG TAGTTCAGGG CGCTCACAAA CGCCCCCTGG TGGTGCCACA 
CCGCCGATTC CCAAAATAAC TATTCAAATA ACAAAAGGGA CTATAACTGG GGGGAAAACA 
TCATCTCTTA GTGGATACAC CTCCAGCAGC TCAGCCACAG GTGGCACAGG AGCAACAGGG 
GGAAACAGTG GCAGCAAAAG TCATCATTCG CACTCCTCCT CCTCCTCTGG GAAGATCAAG 
TCCAAGGAAA GTTCTGCTAC ACCAGGCAGC GCGTCAAAGC CGGGCAGTGC GGGAATAACA 
AGTGGAGTGC CATCCCAGTC TAAAACTCCT TCACAAGGAA TAGGCATTGG TAAACCTGGA 
TCCTCCCCAA TTAATAAACA TGGTTTGTCG GGGCCTGGAA GCAGCGGCAG TGGAATTGTG 
GGAGGCAATA AGACAAAACC TCCAGGGGGC AAGCCTCCTG GGTCTCTTAT GAATCCCAAC 
ATTAAGCCCA ACATATCCCC TTCTCACTCC CGCTCCAGTA GCTCTGGTGA CAAAATGTCT 
TCCCCACTGA AAATGCAGCA AGCCCAAGTT CCAGGAACAC CCCCTTCCTC TAAAGCGAAA 
TCACCAATTG GCTCAGGTGG CAGCAGTTCC GCAGGGTCCA AATTATCCTC CGGCCCAGGC 
ATGAGCTCCC AGAAACCGCT GGGTGGGAGC TCCTCCTCTG GATCCACATC TTCATCATCG 
TCTTCCAGCT CAACATCATC ATCCGGCTCC ATGACTTTTT CAGGAAGCTC GCAGTCTCAG 
TACGGGGGAG GTGGCAGTGG AGGAGGAGGT GGTAGCGCGG CAGGAGGAAG CGGATGTGGA 
GGCGGTGGTA GCAGTGGAGG AAGCGGAGGT GGCACAGTAG CCGGTCAGAA CAATGCAAAC 
AACCCCAACG CCAAGGGAAA ATCCCCCAGT CGAAACAAGA AACCCTCTCT CACAGCAGTC 
ATAGACAAAT TAAAAAGTGT AGGTGGAGGA GGAGTTGGGG AGGATGGTTG TGAGGTTGGG 
CCACCTGTAG GTGGTCCTGG TTCTGGAAGT GTTCCTAGTG GTGGTGGACC TGGAAATGTT 
CCTAGTGGTG GAGCGCTTAA CATTTGTCCT TCTAAACACA TCTCAACCTC TCAAGGTGGT 
GAGTATAAGA GGGAGAAGTC TGAAAAGGAA GCAAAAGCGA AAGTGTCTGT TTCAGGGGGG 
AACAGCGGGG ATAAAAAGCT GATGGACCCC AAAACGGGAG GTGTGGGTAC CACTGGTTTG 
GCTAAAATTA TCATTAGCAA ACCTGATGGT GGCTCACCAA GCATTAAGGC CAAAGGGCCT 
CTCCAGAAAT CAGGAGAAGG GTCCGGTGAC TCAATTCGCC CCCAGATGAC TAGCCTCAAA 
GCTTCCCCCC TTTTCAGTGG CTCAACCCCC AAGCACGACC GCTCCTCCCC GAGCCACAGC 
CGCTCACCTG GATGCACCCC GCTCAACCAC GACAGCGAGA GCGAGTCGGG CAGCAGCTCG 
GTGGCCGAGA AATCCCACCA GAACAGTCCC AGCTCAGACG ACGACCAGAC TATCCGACCG 
CTTCCACCTC AGGACTACTT GAGCTCTATT CCTCTCAGCT CAGGGGAGAA GCACAAGAAA 
CACAAGAAGG AAAAGAAAAA GAAAGAGCGG GAACGGGAAA GAGACAGAGA TCGGGAGAGG 
GACCGGGACA AAGAGAAAAA GAAATCATCC ATGTCCATGG TCCCGTCCTC GCATCCCATC 
AAAGCAGACA GTTGGTCCCG GTCACCCATC TCTGCTTCAG ATTCTTCTTT GTCCATGCTG 
GGTCCAGAGC GTCCCACCCG GCCCAGCCCA TTGTATATGA GAAATGAAGA CGATGACCTC 
ATGGACTCAG CTCTGACTGG CAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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med1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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