LOC101061699 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101061699
Entrez Gene ID	101061699
Full Name	myosin light chain kinase, smooth muscle-like
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029847411.1	XP_029703271.1	myosin light chain kinase, smooth muscle
XM_011621260.1	XP_011619562.1	myosin light chain kinase, smooth muscle-like

Pathways from BioSystems
Gene Ontology

The following LOC101061699 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101061699 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo19047	XM_011621260.1 Latest version!	Takifugu rubripes myosin light chain kinase, smooth muscle-like (LOC101061699), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo42752	XM_029847411.1 Latest version!	Takifugu rubripes myosin light chain kinase (mylk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101061699 ( XM_011621260.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011619562.1 Takifugu rubripes myosin light chain kinase, smooth muscle-like (LOC101061699), mRNA.

Price & Availability↑

CloneID	OTo19047
Clone ID Related Accession (Same CDS sequence)	XM_011621260.1
Accession Version	XM_011621260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	myosin light chain kinase, smooth muscle-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGG AGGGACAAAA ATCAAAGACC TTTGTATCTA CTTTCCGGTT GGTCCTAAAG 
CCAAAAGCCA GCGGGTTGTT AGTTGTGAAG CAAAAAAATG ATGGACTGGA TACAAAAAAC 
AGGACTTTAA CAGGGAGCAG CAACGTGGAA GTCTCATCTT CAACAGGCAA GAGCGCGCCA 
TCCCATAATC CTCTACGTAG AAATGGTGTT GCCAGTCAGT CTCAGTGTTC TACTGGAGAT 
GCCGGCACTC ACACCTGCAG ATCTGACAGC GCTGCTGAGA GGCAGCCAAC CAGCGCTGAT 
GTACACATCA TAGCAACAAG GGAAATTCCT GCAACAAACA AGCAGCTGCA CCAGGGGCTG 
AAGTCAGCTG AAGTCAAAGC TTGGGCCACA AGAGCTTCAA GAGAAAAAAG CCAACAGGAT 
GAAAACTATG ATATCACAGA GCGGCGACGT CAAGCAAAAG CCTCTGCCAA AAAAGAGCCA 
CCAGTACAGT TTCTAAACCC ACCAGAGCAG GTGGAGGTTC GGGTGGGAGA GCGGGCCCGA 
CTACACTGTG AGTTCAGGAG TAGCTCTGTC CCGGTGGCCT GCTGCTGGAT TTACAACCGG 
GACAAGGTGG TGGAAACAGC CCAGCGGGTA TCGGTCAGAA GCAGTGAGAC TCAAAGCAGT 
GTGGACATCT CTGGGGCCGG TCCAGCTGAC GGGGGCTCCT ATACCATCGT AGTACGGAAC 
AAGGAGGGCT CAGCCAATCA CACGGTGTCG CTGACTGTCA TAGACCGACC TGACCCACCA 
GCATCGCAAC CAGTCGTTTC CCAGCTGACA AGCGTGTCCT TGGTCCTGTC CTGGACGGGT 
CCCAGCTACG ACGGAGGCAA AGCCGTGCAG GGCTACATTG TGGAGGTCAG ACCAGAGGGC 
TTGGGTAAGA CCCCAGGTTG GACTGAACTC GCCACCCGTT GCAAAAGCAC GTCCTACCAC 
GTCCGCTCGG GCCTGGACCC TTTGGGAAGG TACCGCTTCC GGGTCAGAGC CTACAACTCG 
GCAGGGATCA GTGAACCAAG CCAGGAATCT GAGTGTGTCA AGATGGCCAT TACAAGGGTA 
TCGAGGCAGC ATCAGCAGCT CGCCTTCTAC GACACAATAA CCATCAATAG CAAACACAGG 
GTGACCGACC ACTACAATGT GCATGAGAAG CTCGGCGTCG GGAAGTTTGG TGAAGTGTTC 
CGATTGTCTC ATAAAGAGAC GGGTCAGGTG TGCGCGGGGA AGTTCTACCG GGCACGGTCT 
GCCAAAGATA GGGCAGCGGC TCGGAAGGAG ATCGAACTCA TGAACTGTCT CCATCACCCC 
AAACTGGTCC AGTGTTTGGC GGCCTACGAC GCGCGCGCGG AGACGGTGAT GGTTCTGGAG 
TACATCGCAG GCGGGGAGCT CTTCGAGCGG CTCGTAGACG ACAACTTTGA GCACACGGAG 
CTCACCAGCG CCCGTTACAT GAGGCAGATC CTGCAAGGCA TGGAGTACGT TCACAAGCAG 
AACATTGTAC ACTTGGACCT CAAACCGGAG AACATCGTGT GCGTAGACAC GACTGGCACG 
CAGATCAAAA TCATCGACTT TGGCCTGGCC AGCAAGCTGG AGGACAATAA GCCTTTGATG 
GTGATGCACG GCACACCCGA ATTCGTGGCC CCTGAGGTAA TCAGCTATGA GCCAGTTGGC 
CTGGAGACGG ACATGTGGAG CATCGGAGTC ATCTGCTTCA TCCTACTGAG CGGAGAATCT 
CCCTTCCAGG GGAACAGCGA TACAGAGACA TTGGCGCTTG TGACGGAGGC CTCCTACGAA 
TTTGACGAGG AGAGTTTCGA GGACATATCG GAGCAAGCAA AAGACTTCAT TCGATCCCTG 
CTGAAGAAAG ACCGCAGATG CCGCCTGTCG TGCACCGAAG CCCTTGTCCA CCCCTGGATG 
ATCTCTTTCA CCCCCTTGAC TCGCAGCTCT ACCAAGTCCC TCAACAAGGA CAAGATGAGG 
CACTTTCTGG CCAAGCGCAA GTGGAAGAAA ACCGGCAAGG CTGTTTTGGC CCTGCAGCGG 
ATGGCTAACC TCTCCAGCAG GCCAGAGTCT CCTGGCAGCT CCTCAGAGGA GCCGGGCTGG 
AGCCAGGAGG CAGAGGAGGC CATAGAGTCG CTGGACAAGC AGCTGAGGAG TGAACCTCGG 
TTCAGGCAGG CCCTGAGGGA CACCATCCAT CCCACAGGAG CTACAGCTCG GCTCACGTGC 
ATTGTCAACG GTTACCCCCA GCCGACGGTG CAGTGGCTTC AGAATGGGAA CCCCGTGACT 
GAATCGAGCA GAACGACAAT GGAGGAAGAG GAAGACGGGC TCTGCGCTCT GGTTCTGGCT 
GATTTGAACC CTTCTGACTC TGGGGTTTAC ACGTGCAAGG CTAGCAACAA GCAGGGAGAA 
GCAACATGCT CAGCCAAACT CAAAGTGGAG ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011619562.1
CDS	286..2721
Translation	MDKEGQKSKTFVSTFRLVLKPKASGLLVVKQKNDGLDTKNRTLTGSSNVEVSSSTGKSAPSHNPLRRNGVASQSQCSTGDAGTHTCRSDSAAERQPTSADVHIIATREIPATNKQLHQGLKSAEVKAWATRASREKSQQDENYDITERRRQAKASAKKEPPVQFLNPPEQVEVRVGERARLHCEFRSSSVPVACCWIYNRDKVVETAQRVSVRSSETQSSVDISGAGPADGGSYTIVVRNKEGSANHTVSLTVIDRPDPPASQPVVSQLTSVSLVLSWTGPSYDGGKAVQGYIVEVRPEGLGKTPGWTELATRCKSTSYHVRSGLDPLGRYRFRVRAYNSAGISEPSQESECVKMAITRVSRQHQQLAFYDTITINSKHRVTDHYNVHEKLGVGKFGEVFRLSHKETGQVCAGKFYRARSAKDRAAARKEIELMNCLHHPKLVQCLAAYDARAETVMVLEYIAGGELFERLVDDNFEHTELTSARYMRQILQGMEYVHKQNIVHLDLKPENIVCVDTTGTQIKIIDFGLASKLEDNKPLMVMHGTPEFVAPEVISYEPVGLETDMWSIGVICFILLSGESPFQGNSDTETLALVTEASYEFDEESFEDISEQAKDFIRSLLKKDRRCRLSCTEALVHPWMISFTPLTRSSTKSLNKDKMRHFLAKRKWKKTGKAVLALQRMANLSSRPESPGSSSEEPGWSQEAEEAIESLDKQLRSEPRFRQALRDTIHPTGATARLTCIVNGYPQPTVQWLQNGNPVTESSRTTMEEEEDGLCALVLADLNPSDSGVYTCKASNKQGEATCSAKLKVEM

Target ORF information:

RefSeq Version	XM_011621260.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes myosin light chain kinase, smooth muscle-like (LOC101061699), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011621260.1

ORF Insert Sequence:

ATGGACAAGG AGGGACAAAA ATCAAAGACC TTTGTATCTA CTTTCCGGTT GGTCCTAAAG 
CCAAAAGCCA GCGGGTTGTT AGTTGTGAAG CAAAAAAATG ATGGACTGGA TACAAAAAAC 
AGGACTTTAA CAGGGAGCAG CAACGTGGAA GTCTCATCTT CAACAGGCAA GAGCGCGCCA 
TCCCATAATC CTCTACGTAG AAATGGTGTT GCCAGTCAGT CTCAGTGTTC TACTGGAGAT 
GCCGGCACTC ACACCTGCAG ATCTGACAGC GCTGCTGAGA GGCAGCCAAC CAGCGCTGAT 
GTACACATCA TAGCAACAAG GGAAATTCCT GCAACAAACA AGCAGCTGCA CCAGGGGCTG 
AAGTCAGCTG AAGTCAAAGC TTGGGCCACA AGAGCTTCAA GAGAAAAAAG CCAACAGGAT 
GAAAACTATG ATATCACAGA GCGGCGACGT CAAGCAAAAG CCTCTGCCAA AAAAGAGCCA 
CCAGTACAGT TTCTAAACCC ACCAGAGCAG GTGGAGGTTC GGGTGGGAGA GCGGGCCCGA 
CTACACTGTG AGTTCAGGAG TAGCTCTGTC CCGGTGGCCT GCTGCTGGAT TTACAACCGG 
GACAAGGTGG TGGAAACAGC CCAGCGGGTA TCGGTCAGAA GCAGTGAGAC TCAAAGCAGT 
GTGGACATCT CTGGGGCCGG TCCAGCTGAC GGGGGCTCCT ATACCATCGT AGTACGGAAC 
AAGGAGGGCT CAGCCAATCA CACGGTGTCG CTGACTGTCA TAGACCGACC TGACCCACCA 
GCATCGCAAC CAGTCGTTTC CCAGCTGACA AGCGTGTCCT TGGTCCTGTC CTGGACGGGT 
CCCAGCTACG ACGGAGGCAA AGCCGTGCAG GGCTACATTG TGGAGGTCAG ACCAGAGGGC 
TTGGGTAAGA CCCCAGGTTG GACTGAACTC GCCACCCGTT GCAAAAGCAC GTCCTACCAC 
GTCCGCTCGG GCCTGGACCC TTTGGGAAGG TACCGCTTCC GGGTCAGAGC CTACAACTCG 
GCAGGGATCA GTGAACCAAG CCAGGAATCT GAGTGTGTCA AGATGGCCAT TACAAGGGTA 
TCGAGGCAGC ATCAGCAGCT CGCCTTCTAC GACACAATAA CCATCAATAG CAAACACAGG 
GTGACCGACC ACTACAATGT GCATGAGAAG CTCGGCGTCG GGAAGTTTGG TGAAGTGTTC 
CGATTGTCTC ATAAAGAGAC GGGTCAGGTG TGCGCGGGGA AGTTCTACCG GGCACGGTCT 
GCCAAAGATA GGGCAGCGGC TCGGAAGGAG ATCGAACTCA TGAACTGTCT CCATCACCCC 
AAACTGGTCC AGTGTTTGGC GGCCTACGAC GCGCGCGCGG AGACGGTGAT GGTTCTGGAG 
TACATCGCAG GCGGGGAGCT CTTCGAGCGG CTCGTAGACG ACAACTTTGA GCACACGGAG 
CTCACCAGCG CCCGTTACAT GAGGCAGATC CTGCAAGGCA TGGAGTACGT TCACAAGCAG 
AACATTGTAC ACTTGGACCT CAAACCGGAG AACATCGTGT GCGTAGACAC GACTGGCACG 
CAGATCAAAA TCATCGACTT TGGCCTGGCC AGCAAGCTGG AGGACAATAA GCCTTTGATG 
GTGATGCACG GCACACCCGA ATTCGTGGCC CCTGAGGTAA TCAGCTATGA GCCAGTTGGC 
CTGGAGACGG ACATGTGGAG CATCGGAGTC ATCTGCTTCA TCCTACTGAG CGGAGAATCT 
CCCTTCCAGG GGAACAGCGA TACAGAGACA TTGGCGCTTG TGACGGAGGC CTCCTACGAA 
TTTGACGAGG AGAGTTTCGA GGACATATCG GAGCAAGCAA AAGACTTCAT TCGATCCCTG 
CTGAAGAAAG ACCGCAGATG CCGCCTGTCG TGCACCGAAG CCCTTGTCCA CCCCTGGATG 
ATCTCTTTCA CCCCCTTGAC TCGCAGCTCT ACCAAGTCCC TCAACAAGGA CAAGATGAGG 
CACTTTCTGG CCAAGCGCAA GTGGAAGAAA ACCGGCAAGG CTGTTTTGGC CCTGCAGCGG 
ATGGCTAACC TCTCCAGCAG GCCAGAGTCT CCTGGCAGCT CCTCAGAGGA GCCGGGCTGG 
AGCCAGGAGG CAGAGGAGGC CATAGAGTCG CTGGACAAGC AGCTGAGGAG TGAACCTCGG 
TTCAGGCAGG CCCTGAGGGA CACCATCCAT CCCACAGGAG CTACAGCTCG GCTCACGTGC 
ATTGTCAACG GTTACCCCCA GCCGACGGTG CAGTGGCTTC AGAATGGGAA CCCCGTGACT 
GAATCGAGCA GAACGACAAT GGAGGAAGAG GAAGACGGGC TCTGCGCTCT GGTTCTGGCT 
GATTTGAACC CTTCTGACTC TGGGGTTTAC ACGTGCAAGG CTAGCAACAA GCAGGGAGAA 
GCAACATGCT CAGCCAAACT CAAAGTGGAG ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101061699 ( XM_029847411.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029703271.1 Takifugu rubripes myosin light chain kinase (mylk), mRNA.

Price & Availability↑

CloneID	OTo42752
Clone ID Related Accession (Same CDS sequence)	XM_029847411.1
Accession Version	XM_029847411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	myosin light chain kinase, smooth muscle
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGG AGGGACAAAA ATCAAAGACC TTTGTATCTA CTTTCCGGTT GGTCCTAAAG 
CCAAAAGCCA GCGGGTTGTT AGTTGTGAAG CAAAAAAATG ATGGACTGGA TACAAAAAAC 
AGGACTTTAA CAGGGAGCAG CAACGTGGAA GTCTCATCTT CAACAGGCAA GAGCGCGCCA 
TCCCATAATC CTCTACGTAG AAATGGTGTT GCCAGTCAGT CTCAGTGTTC TACTGGAGAT 
GCCGGCACTC ACACCTGCAG ATCTGACAGC GCTGCTGAGA GGCGGCCAAC CAGCGCTGAT 
GTACACATCA TAGCAACAAG GGAAATTCCT GCAACAAACA AGCAGCTGCA CCAGGGGCTG 
AAGTCAGCTG AAGTCAAAGC TTGGGCCACA AGAGCTTCAA GAGAAAAAAG CCAACAGGAT 
GAAAACTATG ATATCACAGA GCGGCGACGT CAAGCAAAAG CCTCTGCCAA AAAAGAGCCA 
CCAGTACAGT TTCTAAACCC ACCAGAGCAG GTGGAGGTTC GGGTGGGAGA GCGGGCCCGA 
CTACACTGTG AGTTCAGGAG TAGCTCTGTC CCGGTGGCCT GCTGCTGGAT TTACAACCGG 
GACAAGGTGG TGGAAACAGC CCAGCGGGTA TCGGTCAGAA GCAGTGAGAC TCAAAGCAGT 
GTGGACATCT CTGGGGCCGG TCCAGCTGAC GGGGGCTCCT ATACCATCGT AGTACGGAAC 
AAGGAGGGCT CAGCCAATCA CACGGTGTCG CTGACTGTCA TAGACCGACC TGACCCACCA 
GCATCGCAAC CAGTCGTTTC CCAGCTGACA AGCGTGTCCT TGGTCCTGTC CTGGACGGGT 
CCCAGCTACG ACGGAGGCAA AGCCGTGCAG GGCTACATTG TGGAGGTCAG ACCAGAGGGC 
TTGGGTAAGA CCCCAGGTTG GACTGAACTC GCCACCCGTT GCAAAAGCAC GTCCTACCAC 
GTCCGCTCGG GCCTGGACCC TTTGGGAAGG TACCGCTTCC GGGTCAGAGC CTACAACTCG 
GCAGGGATCA GTGAACCAAG CCAGGAATCT GAGTGTGTCA AGATGGCCAC TACAAGGGTA 
TCGAGGCAGC ATCAGCAGCT CGCCTTCTAC GACACAATAA CCATCAATAG CAAACACAGG 
GTGACCGACC ACTACAATGT GCATGAGAAG CTCGGCGTCG GGAAGTTTGG TGAAGTGTTC 
CGATTGTCTC ATAAAGAGAC GGGTCAGGTG TGCGCGGGGA AGTTCTACCG GGCACGGTCT 
GCCAAAGATA GGGCAGCGGC TCGGAAGGAG ATCGAACTCA TGAACTGTCT CCATCACCCC 
AAACTGGTCC AGTGTTTGGC GGCCTACGAC GCGCGCGCGG AGACGGTGAT GGTTCTGGAG 
TACATCGCAG GCGGGGAGCT CTTCGAGCGG CTCGTAGACG ACAACTTTGA GCACACGGAG 
CTCACCAGCG CCCGTTACAT GAGGCAGATC CTGCAAGGCA TGGAGTACGT TCACAAGCAG 
AACATTGTAC ACTTGGACCT CAAACCGGAG AACATCGTGT GCGTAGACAC GACTGGCACG 
CAGATCAAAA TCATCGACTT TGGCCTGGCC AGCAAGCTGG AGGACAATAA GCCTTTGATG 
GTGATGCACG GCACACCCGA ATTCGTGGCC CCTGAGGTAA TCAGCTATGA GCCAGTTGGC 
CTGGAGACGG ACATGTGGAG CATCGGAGTC ATCTGCTTCA TCCTACTGAG CGGAGAATCT 
CCCTTCCAGG GGAACAGCGA TACAGAGACA TTGGCGCTTG TGACGGAGGC CTCCTACGAA 
TTTGACGAGG AGAGTTTCGA GGACATATCG GAGCAAGCAA AAGACTTCAT TCGATCCCTG 
CTGAAGAAAG ACCGCAGATG CCGCCTGTCG TGCACCGAAG CCCTTGTCCA CCCCTGGATG 
ATCTCTTTCA CCCCCTTGAC TCGCAGCTCT ACCAAGTCCC TCAACAAGGA CAAGATGAGG 
CACTTTCTGG CCAAGCGCAA GTGGAAGAAA ACCGGCAAGG CTGTTTTGGC CCTGCAGCGG 
ATGGCTAACC TCTCCAGCAG GCCAGAGTCT CCTGGCAGCT CCTCAGAGGA GCCGGGCTGG 
AGCCAGGAGG CAGAGGAGGC CATAGAGTCG CTGGACAAGC AGCTGAGGAG TGAACCTCGG 
TTCAGGCAGG CCCTGAGGGA CACCATCCAT CCCACAGGAG CTACAGCTCG GCTCACGTGC 
ATTGTCAACG GTTACCCCCA GCCGACGGTG CAGTGGCTTC AGAATGGGAA CCCCGTGACT 
GAATCGAGCA GAACGACAAT GGAGGAAGAG GAAGACGGGC TCTGCGCTCT GGTTCTGGCT 
GATTTGAACC CTTCTGACTC TGGGGTTTAC ACGTGCAAGG CTAGCAACAA GCAGGGAGAA 
GCAACATGCT CAGCCAAACT CAAAGTGGGG ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029703271.1
CDS	310..2745
Translation	MDKEGQKSKTFVSTFRLVLKPKASGLLVVKQKNDGLDTKNRTLTGSSNVEVSSSTGKSAPSHNPLRRNGVASQSQCSTGDAGTHTCRSDSAAERRPTSADVHIIATREIPATNKQLHQGLKSAEVKAWATRASREKSQQDENYDITERRRQAKASAKKEPPVQFLNPPEQVEVRVGERARLHCEFRSSSVPVACCWIYNRDKVVETAQRVSVRSSETQSSVDISGAGPADGGSYTIVVRNKEGSANHTVSLTVIDRPDPPASQPVVSQLTSVSLVLSWTGPSYDGGKAVQGYIVEVRPEGLGKTPGWTELATRCKSTSYHVRSGLDPLGRYRFRVRAYNSAGISEPSQESECVKMATTRVSRQHQQLAFYDTITINSKHRVTDHYNVHEKLGVGKFGEVFRLSHKETGQVCAGKFYRARSAKDRAAARKEIELMNCLHHPKLVQCLAAYDARAETVMVLEYIAGGELFERLVDDNFEHTELTSARYMRQILQGMEYVHKQNIVHLDLKPENIVCVDTTGTQIKIIDFGLASKLEDNKPLMVMHGTPEFVAPEVISYEPVGLETDMWSIGVICFILLSGESPFQGNSDTETLALVTEASYEFDEESFEDISEQAKDFIRSLLKKDRRCRLSCTEALVHPWMISFTPLTRSSTKSLNKDKMRHFLAKRKWKKTGKAVLALQRMANLSSRPESPGSSSEEPGWSQEAEEAIESLDKQLRSEPRFRQALRDTIHPTGATARLTCIVNGYPQPTVQWLQNGNPVTESSRTTMEEEEDGLCALVLADLNPSDSGVYTCKASNKQGEATCSAKLKVGM

Target ORF information:

RefSeq Version	XM_029847411.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes myosin light chain kinase (mylk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029847411.1

ORF Insert Sequence:

ATGGACAAGG AGGGACAAAA ATCAAAGACC TTTGTATCTA CTTTCCGGTT GGTCCTAAAG 
CCAAAAGCCA GCGGGTTGTT AGTTGTGAAG CAAAAAAATG ATGGACTGGA TACAAAAAAC 
AGGACTTTAA CAGGGAGCAG CAACGTGGAA GTCTCATCTT CAACAGGCAA GAGCGCGCCA 
TCCCATAATC CTCTACGTAG AAATGGTGTT GCCAGTCAGT CTCAGTGTTC TACTGGAGAT 
GCCGGCACTC ACACCTGCAG ATCTGACAGC GCTGCTGAGA GGCGGCCAAC CAGCGCTGAT 
GTACACATCA TAGCAACAAG GGAAATTCCT GCAACAAACA AGCAGCTGCA CCAGGGGCTG 
AAGTCAGCTG AAGTCAAAGC TTGGGCCACA AGAGCTTCAA GAGAAAAAAG CCAACAGGAT 
GAAAACTATG ATATCACAGA GCGGCGACGT CAAGCAAAAG CCTCTGCCAA AAAAGAGCCA 
CCAGTACAGT TTCTAAACCC ACCAGAGCAG GTGGAGGTTC GGGTGGGAGA GCGGGCCCGA 
CTACACTGTG AGTTCAGGAG TAGCTCTGTC CCGGTGGCCT GCTGCTGGAT TTACAACCGG 
GACAAGGTGG TGGAAACAGC CCAGCGGGTA TCGGTCAGAA GCAGTGAGAC TCAAAGCAGT 
GTGGACATCT CTGGGGCCGG TCCAGCTGAC GGGGGCTCCT ATACCATCGT AGTACGGAAC 
AAGGAGGGCT CAGCCAATCA CACGGTGTCG CTGACTGTCA TAGACCGACC TGACCCACCA 
GCATCGCAAC CAGTCGTTTC CCAGCTGACA AGCGTGTCCT TGGTCCTGTC CTGGACGGGT 
CCCAGCTACG ACGGAGGCAA AGCCGTGCAG GGCTACATTG TGGAGGTCAG ACCAGAGGGC 
TTGGGTAAGA CCCCAGGTTG GACTGAACTC GCCACCCGTT GCAAAAGCAC GTCCTACCAC 
GTCCGCTCGG GCCTGGACCC TTTGGGAAGG TACCGCTTCC GGGTCAGAGC CTACAACTCG 
GCAGGGATCA GTGAACCAAG CCAGGAATCT GAGTGTGTCA AGATGGCCAC TACAAGGGTA 
TCGAGGCAGC ATCAGCAGCT CGCCTTCTAC GACACAATAA CCATCAATAG CAAACACAGG 
GTGACCGACC ACTACAATGT GCATGAGAAG CTCGGCGTCG GGAAGTTTGG TGAAGTGTTC 
CGATTGTCTC ATAAAGAGAC GGGTCAGGTG TGCGCGGGGA AGTTCTACCG GGCACGGTCT 
GCCAAAGATA GGGCAGCGGC TCGGAAGGAG ATCGAACTCA TGAACTGTCT CCATCACCCC 
AAACTGGTCC AGTGTTTGGC GGCCTACGAC GCGCGCGCGG AGACGGTGAT GGTTCTGGAG 
TACATCGCAG GCGGGGAGCT CTTCGAGCGG CTCGTAGACG ACAACTTTGA GCACACGGAG 
CTCACCAGCG CCCGTTACAT GAGGCAGATC CTGCAAGGCA TGGAGTACGT TCACAAGCAG 
AACATTGTAC ACTTGGACCT CAAACCGGAG AACATCGTGT GCGTAGACAC GACTGGCACG 
CAGATCAAAA TCATCGACTT TGGCCTGGCC AGCAAGCTGG AGGACAATAA GCCTTTGATG 
GTGATGCACG GCACACCCGA ATTCGTGGCC CCTGAGGTAA TCAGCTATGA GCCAGTTGGC 
CTGGAGACGG ACATGTGGAG CATCGGAGTC ATCTGCTTCA TCCTACTGAG CGGAGAATCT 
CCCTTCCAGG GGAACAGCGA TACAGAGACA TTGGCGCTTG TGACGGAGGC CTCCTACGAA 
TTTGACGAGG AGAGTTTCGA GGACATATCG GAGCAAGCAA AAGACTTCAT TCGATCCCTG 
CTGAAGAAAG ACCGCAGATG CCGCCTGTCG TGCACCGAAG CCCTTGTCCA CCCCTGGATG 
ATCTCTTTCA CCCCCTTGAC TCGCAGCTCT ACCAAGTCCC TCAACAAGGA CAAGATGAGG 
CACTTTCTGG CCAAGCGCAA GTGGAAGAAA ACCGGCAAGG CTGTTTTGGC CCTGCAGCGG 
ATGGCTAACC TCTCCAGCAG GCCAGAGTCT CCTGGCAGCT CCTCAGAGGA GCCGGGCTGG 
AGCCAGGAGG CAGAGGAGGC CATAGAGTCG CTGGACAAGC AGCTGAGGAG TGAACCTCGG 
TTCAGGCAGG CCCTGAGGGA CACCATCCAT CCCACAGGAG CTACAGCTCG GCTCACGTGC 
ATTGTCAACG GTTACCCCCA GCCGACGGTG CAGTGGCTTC AGAATGGGAA CCCCGTGACT 
GAATCGAGCA GAACGACAAT GGAGGAAGAG GAAGACGGGC TCTGCGCTCT GGTTCTGGCT 
GATTTGAACC CTTCTGACTC TGGGGTTTAC ACGTGCAAGG CTAGCAACAA GCAGGGAGAA 
GCAACATGCT CAGCCAAACT CAAAGTGGGG ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101061699 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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