UMAG_06414 cDNA ORF clone, Ustilago maydis 521

Gene
Clones
gRNAs

Summary

Gene Symbol	UMAG_06414
Entrez Gene ID	23566013
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Ustilago maydis 521

mRNA and Protein(s)

mRNA	Protein	Name
XM_011394396.1	XP_011392698.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis.
Kämper J, Kahmann R, Bölker M, Ma LJ, Brefort T, Saville BJ, Banuett F, Kronstad JW, Gold SE, Müller O, Perlin MH, Wösten HA, de Vries R, Ruiz-Herrera J, Reynaga-Peña CG, Snetselaar K, McCann M, Pérez-Martín J, Feldbrügge M, Basse CW, Steinberg G, Ibeas JI, Holloman W, Guzman P, Farman M, Stajich JE, Sentandreu R, González-Prieto JM, Kennell JC, Molina L, Schirawski J, Mendoza-Mendoza A, Greilinger D, Münch K, Rössel N, Scherer M, Vranes M, Ladendorf O, Vincon V, Fuchs U, Sandrock B, Meng S, Ho EC, Cahill MJ, Boyce KJ, Klose J, Klosterman SJ, Deelstra HJ, Ortiz-Castellanos L, Li W, Sanchez-Alonso P, Schreier PH, Häuser-Hahn I, Vaupel M, Koopmann E, Friedrich G, Voss H, Schlüter T, Margolis J, Platt D, Swimmer C, Gnirke A, Chen F, Vysotskaia V, Mannhaupt G, Güldener U, Münsterkötter M, Haase D, Oesterheld M, Mewes HW, Mauceli EW, DeCaprio D, Wade CM, Butler J, Young S, Jaffe DB, Calvo S, Nusbaum C, Galagan J, Birren BW
Nature444(7115)97-101(2006 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UMAG_06414 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UMAG_06414 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OUd00113	XM_011394396.1 Latest version!	Ustilago maydis 521 hypothetical protein (UMAG_06414), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UMAG_06414 ( XM_011394396.1 ) cDNA ORF clone, Ustilago maydis 521 -> XP_011392698.1 Ustilago maydis 521 hypothetical protein (UMAG_06414), partial mRNA.

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CloneID	OUd00113
Clone ID Related Accession (Same CDS sequence)	XM_011394396.1
Accession Version	XM_011394396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522598400000
Organism	Ustilago maydis 521
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026500). COMPLETENESS: incomplete on both ends.

ATGAGCGCTG CTATCCTCCC CTCGTCAGCC ATATCTTTCG ACTCTACATT CTCATCCTTC 
GACAACGCCG TTCCCAGCGA TGACTCTCAA AAGTTTGATC CTCCCTCCCC ACCTCCCCTG 
CCACGCGCTT CCGTGATTGT CTTTGGCTCA CAGGGCTCGG ATTTCCTAAA CGCTTTCGAC 
GCCATCATCG GCCTTTCACA TTACCATGCC GAGCTCGCTG CTTTCCTCTC CTTGGCTCTT 
ACAGCCGTCC GCGCCGAGCT CCAAAGCGTC GCGAGTCGTT ATCATCACGT GCCCGAATCG 
GATGAGTTCA CCACAACAGG CCAAAGAAAA GCCGAAGTGG AGCTCTTGGC CTCCCTTCCT 
CCCTTTGAGC CTTTTGCTGG GCTCAGGACA CTCGTAGACT TTCATCGTCG TAGCCAGCAG 
AAAGATCCAA CGATCAACGG TGTGCTCCTC TGTCTTCTCC AAACCGCAAG CGTCGTCGCA 
GTTTGCTGCG TGGCACACCA TGACCAGGAT GACCCGCATG CACCCTCGAC CGCATCTGGC 
CTCAAGCGAG CCTGGAAAGC TTTGACGCAA CCTTGCTCAC ACTTGCTCGG CTACTGCACC 
GGCGCTCTCT CTGCCTTCAG CCTTCGCGAG ATGGTCGAGC ACGAGGCCAC AGCATCATCG 
GTCGACATCT GGGCTTACGC AAAGGATGCG ATAAGAGCTA TTCGTATCTG CTTTTGGATC 
AGCCTGCGCT CAGCGCACGC TCGTCTTCGT CTAATCTCGG CAGACGATTC TGCAACTGCA 
TCCGATCCCT GGAGCTTCCT CGTCTCGGTC AAAGATCCAG ACCTTATTCA AAATGTCTAC 
CTGTATCTTG CCCGGTTCAA CGATCTTGAG CAACAAAGGC CACTCGATAA ACCAATTCCC 
CTCGTTGTCT CTGCCAGAGC ACTCAATCAG ATCTCAATTG CTGGTCTTCC AAGACAGCTG 
CAACGCTTCA AGGCCTACTT ACTCGAGCAC CTCGGTTCCA AATGCAAGAT GTTTTCTCTA 
AAGTTGTTCT CTCCTTATCA TATGTCAGAT CTCGCGCCCG AAGCAGATCT GGTCATGCGC 
GATCTCGAGC ATCGAAAGTT GGTCGACCCT TCCAACTTGT CACCAAGCCT CAAAAGCTTA 
TGGGAGACGA AAAACGCCAA CATTCTATCC GAGGCCAATT TGCAGGAAGC CTTGCGTGTT 
CTTGTAGCGA CCAATCTGTG TGCCACTGCA GACTGGAATG CCATGGTCGA CCAGTTGGTT 
TCTCTGGAAG GCGAGCTCCC TCGTTCCATG CACCCGGAAG ACCGGGTCGT TGTCTCCTTC 
TGTCCCGGTG CCAGCCTTGG CGCCGACATT GCAATGCGTC TCTCCAGTCT CCACGACTCG 
TGTGATTCGA ATGGGTCAGT CGTCCATATC GATGTGTCTG CTGCCCTTTC GGAGCACTTG 
ATGCATCCGT ACTATTCAGG ACTCGATGTC CCGTTTGTAT CCCAATCCGA CGTCAATGGC 
GAAGAGGTTG TGATTGTATC AATGGCTTGT CGTTTCCCTG GCGAGGTGTG CAACCCCGAC 
CAATTTTGGA CTTGCCTTGA AACAGGAAGA TCGACGGTCA CAGAAATTCC CAAGCACTTG 
TTTGACATTG ATGCATACTA TGGCGACGGC GTCAACCAGA CTATGGCAAG ACACATGCAT 
GCCTTACCCG AGAACGTGGT CAAGTCGATG GATGCACGTC TGTTCAGCAT GTCTCCAAAA 
GAGATCGAGC AGCTTGATCC TCAGCACCGT CTGGTGATGC TTTGTTCTTA CGAGGCACTC 
GAACGGGCAG GATACTCGGC CGAAGCCAAC TCTCCTTCGT CTTTCGACGG CAAGCGTATC 
GCCGTCTGTA TGGCCGCGAG CTGGGACGAC TACCGAGAAA ACGCCAGTTG GAACATTGGC 
AGCTACTTTA TCACTGGCAA CATCCGTGCC TTCATCCCTG GTCACGTGTC CTTCTCACTC 
AACTGGGAAG GACCCAGCGT ATCGGTCGAT TCACTCGAAT GTTCGGCGGT ATCAGCATTA 
CAGTGGTCTA GACGCGCACT CCTGAGCGGA CAGTGCGATG TGGCACTTGC TGGTGCTGTC 
AACGTTCTGA CACAGCCGCA AATGTTCATC GCTATGGACA AAGAAGGTAT CCTCTCTCGT 
ACTGGAACGA ATGCTACGTT CTCCTCCAAA CTCGATGGAA AGACGAGAGG CGACGGATGC 
GGTGTTCTGC TGTTGAAGCG ACGCTCAACA GCACTTCGCG ATGGCGACAA GATCCTCGCC 
ACTCTGCCAG CTGCCAGGAC CACTTACCAT GGTAGTGCCC ATGAGAGTGA GGAAGCTAGC 
ACCAACCAGT CACGCTTTCT GGCTCGAGTC ATGTCCGAAG CAAGTGTTCG TGCATCCGAT 
CTCATACACA TCGAAGCAAG CGGATTCCAT ACACAACAAG CAGAGGCCGC CGAGTTCGAA 
TCTTTCAGCC GTCTGCTACC TCAATCCCAA GGTGATCGTT CATCCCAGGT CAGAGATAGG 
ATCTCGGTCG CGAGTTCTCG GCCGAACATT GGTGCAGGCG AAGCGGTGTC TGCCATGGCG 
TCGGTGATTA AGGCTGTGCT CATGTTTCAG CAAGGCAGTG TGCCACGACA GATCTCCATC 
TCGGATCCGT CCGAGCTTCA GCCCAGCATT ACTGCGATCT GCGCCGGTTC ATCTTTGTTT 
GTTCCAACGC AAGCGCGAAG ACTGCCGTCC GATAGAGACC ACGAAGGCCG ACACGTTATC 
CTGGTCAACA GTCTCGCGAG CACAAATTGC CATGGAGTCG TGCTGGTCGG TGCACCAACG 
ACTGACGACG TTGCACCACT GCAGATTCGT TCCAAAGCAC TTGACGCCAC TTTTACACGC 
ACAGAAGGGA CCTGGGTCTT TCTGCTCTCC GCAAAGACGA GGGAGTCAGC AGAGATGCTA 
AGAAAGGCCC TGATCGACTA TCTTCGCCGT GACGTGAATC TTGCCGATTT GAGCTACACT 
CTTACTTGTC GCAGGACGCA TCACCCGTTC CGTCTGAGCG TGGTAGCCTC GGACAAAGAT 
GAGCTGGTTC GGTCTCTGCG TACCGCTGAG TTCATCGAAG CTAAAGCGCT GGCCGATCTT 
CCGGCGTTTG GCCTCGTCCT TCGCCATCCT GAGCGGTCAG CTTGTCAAAA CATGGAAGGA 
TCGTTGGACC TTATCGTTGG ACTACGAGAT CGCTTCGAAG AACTGACATC AGTTATGGGT 
TACTCCGAAA TTCCAGCCGA TACAGAGCGG AAGCTGAATT TGAAGCAGGT TTGTCTAGCT 
TTGCTTCTCG ACGACTGCGG CGTCCACCCA TTGTCTTTCC ATGGCAGCTA TGATCTCATG 
CAGCTCTTGC AACGCTCGTT CCCAGCAGCG AAAGAAAGCG ATCTGTCCAA ACGCTTTACG 
TGGATTCACA TCGGAAGCCT GATCAGCATA TCCACCACCG AAGCCGCGGA ACAAGCAAAG 
CCTGCCGGTT CCGTGGCCGA CGGTCAGAAC CAGCAAAGGT CATTGCTCTC CACGCTTGGG 
TCTCTGCATC AAAAAGGTCA CTGCATTCGT TGGATCGAGT ACTTCCGTCC TCTTCTGGCG 
AAACTTAACT TTATCGAGAC GCTGCCCACA TATCTTTTCA ACCTTCAGCA GCACTGGATG 
GAGTATCACG ACCGCGATCT TCTTCCGCGA TCGGCTTCCA AGGAGGGTCA AGGTGGACAG 
AGGATCCGGC ACATCAGTGA TGGCGTTCTT TACTCGCCTT CAAGCCAAGT CGAAGCTTGG 
GTATCGCCGT CGCAGCCTTT GCTAAGCGAG CAAACGGCAG CAGACGACAG ATTGAGGACG 
TACAGCTCTA GCCTTTTCGA TGAAGCTGCT GCAGCCTTGC TGAGCGAAGC TAGCCCGGGA 
GCTCTGATCG CGGAGCTGGT AGTGGAAGCG ATCAAGGAAG CTTGCGAATC GATTGAGGGA 
ACCGTCGAAG CCTATGAGCA TCGCCCTGTT CGGCTTTATG AATTGTTCTT GGCCGAAGTG 
GTCAAGCAAT GGCCAGCCAC TGGCAAGGTC AAGCTTTCAG TGAAGTCGGT GCAGCAGACA 
GGGAATCGAA GCGAAGGGAC AGTTCAAATC TCTTTTGACG CTACTACTTC GCCTGTCGGC 
GAATGCCGAT ATGAATGGAT CACGCAGACA AACATGCATC AGAGATGGTG GAAGCTGCAG 
AAGCTGCTCG CAGAGAAGAT TTCGACGATC AAAGACAAAG GCGAGCTCTT CTCTCCAAAG 
CTCATCTATA AAACCCTCGG AGTTGACCCC ACGACGACTA CGATGTCGAT CCACAGCGCG 
TACCTCGACT GTCAATCGAG CGAAGCGGTC GTTTGCAACA CCATTCGTAC GCACACATCT 
GCTGTTGGAC AGATTGCACT GCCGCAGCTT CTGTCCAGTC TCGAGGAGAT TGCACGCTGG 
TTTGTTGGCG ACAAACCAAG CTCGGCTGAT GGCAAACTCA CGTTCATCGG CGTGGACGAG 
CTGCTCATTT GTGCCGATTG GCTTGATAGC CTGACTCGAC CAGCAGGTGC GTCTGTCAGC 
TACACGGCCT ACATCTCGCA GAGTCACACA GATGGTTCAT TGCGTGCTTC TACAGATGGG 
CTGGAGCTTG ACATTTTGAT CCTCGATGGC ATGCACAAGG TCGTTGGGGA GCTCAGCCGT 
CTTCGACTCG CAAAGTGCAA CAGCACTGCG AAGAAGACGA CGTCGAAGTG TGCTTCAGAG 
TCGACTCTGG CGCTTTCCAG TGGTGTCAAG ACAGTGTCAG GGTCATTTCC GGTGCAAGAG 
CCTGTTGAGC AACACAAGGA GCCCCGATCG ACCTCTGCAA AGCCTGTAGC AGGGAGACAA 
TCGAGCCGAG CAATGGGACT GCACGCCAAA GTGCTCGATG TGCTTGCTTC CGAACTGGGC 
GTTGCGGTCG AGGAGATGAA GCCCACCGTT AAGTTTGCCG ATCTCGGACT GGACAGTCTC 
ATGTCACTAG TTTGCATCTC TACTCTAGAG ACTATGAACC TTGGGCTTGA CATCCCGCAG 
TCGTTGTTTA TGGAGTGCGA CAGCCCTGCA GAGCTGTTGC AGTTTATCCG GGAGCAGGTC 
AGTGACGGAA CTGACGTTGG CCAGTTGGGG CCAGACGAGT CAACAAACGC AACGGAGCAA 
GTTCAGGGAA GCGATGCTGT GGTTCAATTG TCGCCGAGCG CCAACGCAGC TGAGACTCAG 
CTGACTGCGA CGACGGGCCA GGGCAGCTCT GCGGTGGAAG AGGCGATGAA GGCGATCCGA 
TGCACCGTTG AGACGGAGCT CGGTGTTGAC CTAGGAAGCA TCGATGAGGA TGCCAACTTG 
GCCGATCTGG GCATGGACTC GCTCATGTCG CTGCTCGTGC TTGGAAATTT GTCGGGAACG 
CTGCCGATCG AGCTGCCTTC GTCGCTTTTT ATGGATTGTT CATCGCTTGG CGAGATCCGG 
TCTTTTATGA CGAGCCAACT CGGAGCTGCA CGCGACTGTG CTCGCTCGGA CACTGTGGAC 
CAGCGAGATT CGAACTCGCA AATCGCGTTC TTTCTTCCAC CTGTCAAGAA ACCAATCCTC 
ATCCGCGAAG GGAGCAGCTC TGGCATGGAC CCGCCGTTGT TCCTTCTGCC CGAGGGAGCA 
GGCATGGCGA CGGTCTACCA ACAGTTCCCA GGCATTGATC GAGCGATCTA CTCGATCAAT 
TCGCCGTTTC TAGCCGACAC CTCGGCATGG ACGGGCGGGA TCGCCCAGAT TGCGCGGTAC 
TATCTCGAGA GTATCAAGCT AATCCAACCG GTGGGTCCGT GGCTGGTGGG AGGGTGGAGT 
TTCGGTGGAA TGGCTGCGTT TGAGATCGCG CGTTTGCTCG CTGACTGTGG TGGAGACAAG 
GACCGAGTCG CTGGACTATT CATTCTCGAC TCGCCCAGTC CCGAATATCC TCCTCTACCG 
ATGAGCATTC TCGACTGGAT CAACAGCGCT CCCGAAGTGC GCGACATGGC GCCGCCCACC 
ATGTCTGCCA AGCTCATTGC TCACTTCAGG GCTACTGTCG ATAGCCTCGT TGGCTGGGAG 
CCGACGACGA TGCGTGCCGC CGACGAGGCG AGCAAACCGC TACCGAAAAC GTGGTACATT 
GTAGCGGAAC AAGCGCTGCC TGGCAAGATG GAGGATGTCA AAGAGCTCAA TGAAACCGTC 
AGGTGGCTCT TCCGATCGAA GCGCGCAGAG AAGAGTGGCG CAGACGGCTG GCAGCACTTG 
ATCCCACACC ATCTCACAGT GATCGGCGTG GCTGAAGCAA ACCACTTCAC CATGGTCAGA 
CGTCCTAACG TCGGTCAGGT CGCACACATC TTGCAGAACG CCTGTCGGAA TGCCTTGCTC 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011392698.1
CDS	1..6366
Translation	MSAAILPSSAISFDSTFSSFDNAVPSDDSQKFDPPSPPPLPRASVIVFGSQGSDFLNAFDAIIGLSHYHAELAAFLSLALTAVRAELQSVASRYHHVPESDEFTTTGQRKAEVELLASLPPFEPFAGLRTLVDFHRRSQQKDPTINGVLLCLLQTASVVAVCCVAHHDQDDPHAPSTASGLKRAWKALTQPCSHLLGYCTGALSAFSLREMVEHEATASSVDIWAYAKDAIRAIRICFWISLRSAHARLRLISADDSATASDPWSFLVSVKDPDLIQNVYLYLARFNDLEQQRPLDKPIPLVVSARALNQISIAGLPRQLQRFKAYLLEHLGSKCKMFSLKLFSPYHMSDLAPEADLVMRDLEHRKLVDPSNLSPSLKSLWETKNANILSEANLQEALRVLVATNLCATADWNAMVDQLVSLEGELPRSMHPEDRVVVSFCPGASLGADIAMRLSSLHDSCDSNGSVVHIDVSAALSEHLMHPYYSGLDVPFVSQSDVNGEEVVIVSMACRFPGEVCNPDQFWTCLETGRSTVTEIPKHLFDIDAYYGDGVNQTMARHMHALPENVVKSMDARLFSMSPKEIEQLDPQHRLVMLCSYEALERAGYSAEANSPSSFDGKRIAVCMAASWDDYRENASWNIGSYFITGNIRAFIPGHVSFSLNWEGPSVSVDSLECSAVSALQWSRRALLSGQCDVALAGAVNVLTQPQMFIAMDKEGILSRTGTNATFSSKLDGKTRGDGCGVLLLKRRSTALRDGDKILATLPAARTTYHGSAHESEEASTNQSRFLARVMSEASVRASDLIHIEASGFHTQQAEAAEFESFSRLLPQSQGDRSSQVRDRISVASSRPNIGAGEAVSAMASVIKAVLMFQQGSVPRQISISDPSELQPSITAICAGSSLFVPTQARRLPSDRDHEGRHVILVNSLASTNCHGVVLVGAPTTDDVAPLQIRSKALDATFTRTEGTWVFLLSAKTRESAEMLRKALIDYLRRDVNLADLSYTLTCRRTHHPFRLSVVASDKDELVRSLRTAEFIEAKALADLPAFGLVLRHPERSACQNMEGSLDLIVGLRDRFEELTSVMGYSEIPADTERKLNLKQVCLALLLDDCGVHPLSFHGSYDLMQLLQRSFPAAKESDLSKRFTWIHIGSLISISTTEAAEQAKPAGSVADGQNQQRSLLSTLGSLHQKGHCIRWIEYFRPLLAKLNFIETLPTYLFNLQQHWMEYHDRDLLPRSASKEGQGGQRIRHISDGVLYSPSSQVEAWVSPSQPLLSEQTAADDRLRTYSSSLFDEAAAALLSEASPGALIAELVVEAIKEACESIEGTVEAYEHRPVRLYELFLAEVVKQWPATGKVKLSVKSVQQTGNRSEGTVQISFDATTSPVGECRYEWITQTNMHQRWWKLQKLLAEKISTIKDKGELFSPKLIYKTLGVDPTTTTMSIHSAYLDCQSSEAVVCNTIRTHTSAVGQIALPQLLSSLEEIARWFVGDKPSSADGKLTFIGVDELLICADWLDSLTRPAGASVSYTAYISQSHTDGSLRASTDGLELDILILDGMHKVVGELSRLRLAKCNSTAKKTTSKCASESTLALSSGVKTVSGSFPVQEPVEQHKEPRSTSAKPVAGRQSSRAMGLHAKVLDVLASELGVAVEEMKPTVKFADLGLDSLMSLVCISTLETMNLGLDIPQSLFMECDSPAELLQFIREQVSDGTDVGQLGPDESTNATEQVQGSDAVVQLSPSANAAETQLTATTGQGSSAVEEAMKAIRCTVETELGVDLGSIDEDANLADLGMDSLMSLLVLGNLSGTLPIELPSSLFMDCSSLGEIRSFMTSQLGAARDCARSDTVDQRDSNSQIAFFLPPVKKPILIREGSSSGMDPPLFLLPEGAGMATVYQQFPGIDRAIYSINSPFLADTSAWTGGIAQIARYYLESIKLIQPVGPWLVGGWSFGGMAAFEIARLLADCGGDKDRVAGLFILDSPSPEYPPLPMSILDWINSAPEVRDMAPPTMSAKLIAHFRATVDSLVGWEPTTMRAADEASKPLPKTWYIVAEQALPGKMEDVKELNETVRWLFRSKRAEKSGADGWQHLIPHHLTVIGVAEANHFTMVRRPNVGQVAHILQNACRNALLN

Target ORF information:

RefSeq Version	XM_011394396.1
Organism	Ustilago maydis 521
Definition	Ustilago maydis 521 hypothetical protein (UMAG_06414), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394396.1

ORF Insert Sequence:

ATGAGCGCTG CTATCCTCCC CTCGTCAGCC ATATCTTTCG ACTCTACATT CTCATCCTTC 
GACAACGCCG TTCCCAGCGA TGACTCTCAA AAGTTTGATC CTCCCTCCCC ACCTCCCCTG 
CCACGCGCTT CCGTGATTGT CTTTGGCTCA CAGGGCTCGG ATTTCCTAAA CGCTTTCGAC 
GCCATCATCG GCCTTTCACA TTACCATGCC GAGCTCGCTG CTTTCCTCTC CTTGGCTCTT 
ACAGCCGTCC GCGCCGAGCT CCAAAGCGTC GCGAGTCGTT ATCATCACGT GCCCGAATCG 
GATGAGTTCA CCACAACAGG CCAAAGAAAA GCCGAAGTGG AGCTCTTGGC CTCCCTTCCT 
CCCTTTGAGC CTTTTGCTGG GCTCAGGACA CTCGTAGACT TTCATCGTCG TAGCCAGCAG 
AAAGATCCAA CGATCAACGG TGTGCTCCTC TGTCTTCTCC AAACCGCAAG CGTCGTCGCA 
GTTTGCTGCG TGGCACACCA TGACCAGGAT GACCCGCATG CACCCTCGAC CGCATCTGGC 
CTCAAGCGAG CCTGGAAAGC TTTGACGCAA CCTTGCTCAC ACTTGCTCGG CTACTGCACC 
GGCGCTCTCT CTGCCTTCAG CCTTCGCGAG ATGGTCGAGC ACGAGGCCAC AGCATCATCG 
GTCGACATCT GGGCTTACGC AAAGGATGCG ATAAGAGCTA TTCGTATCTG CTTTTGGATC 
AGCCTGCGCT CAGCGCACGC TCGTCTTCGT CTAATCTCGG CAGACGATTC TGCAACTGCA 
TCCGATCCCT GGAGCTTCCT CGTCTCGGTC AAAGATCCAG ACCTTATTCA AAATGTCTAC 
CTGTATCTTG CCCGGTTCAA CGATCTTGAG CAACAAAGGC CACTCGATAA ACCAATTCCC 
CTCGTTGTCT CTGCCAGAGC ACTCAATCAG ATCTCAATTG CTGGTCTTCC AAGACAGCTG 
CAACGCTTCA AGGCCTACTT ACTCGAGCAC CTCGGTTCCA AATGCAAGAT GTTTTCTCTA 
AAGTTGTTCT CTCCTTATCA TATGTCAGAT CTCGCGCCCG AAGCAGATCT GGTCATGCGC 
GATCTCGAGC ATCGAAAGTT GGTCGACCCT TCCAACTTGT CACCAAGCCT CAAAAGCTTA 
TGGGAGACGA AAAACGCCAA CATTCTATCC GAGGCCAATT TGCAGGAAGC CTTGCGTGTT 
CTTGTAGCGA CCAATCTGTG TGCCACTGCA GACTGGAATG CCATGGTCGA CCAGTTGGTT 
TCTCTGGAAG GCGAGCTCCC TCGTTCCATG CACCCGGAAG ACCGGGTCGT TGTCTCCTTC 
TGTCCCGGTG CCAGCCTTGG CGCCGACATT GCAATGCGTC TCTCCAGTCT CCACGACTCG 
TGTGATTCGA ATGGGTCAGT CGTCCATATC GATGTGTCTG CTGCCCTTTC GGAGCACTTG 
ATGCATCCGT ACTATTCAGG ACTCGATGTC CCGTTTGTAT CCCAATCCGA CGTCAATGGC 
GAAGAGGTTG TGATTGTATC AATGGCTTGT CGTTTCCCTG GCGAGGTGTG CAACCCCGAC 
CAATTTTGGA CTTGCCTTGA AACAGGAAGA TCGACGGTCA CAGAAATTCC CAAGCACTTG 
TTTGACATTG ATGCATACTA TGGCGACGGC GTCAACCAGA CTATGGCAAG ACACATGCAT 
GCCTTACCCG AGAACGTGGT CAAGTCGATG GATGCACGTC TGTTCAGCAT GTCTCCAAAA 
GAGATCGAGC AGCTTGATCC TCAGCACCGT CTGGTGATGC TTTGTTCTTA CGAGGCACTC 
GAACGGGCAG GATACTCGGC CGAAGCCAAC TCTCCTTCGT CTTTCGACGG CAAGCGTATC 
GCCGTCTGTA TGGCCGCGAG CTGGGACGAC TACCGAGAAA ACGCCAGTTG GAACATTGGC 
AGCTACTTTA TCACTGGCAA CATCCGTGCC TTCATCCCTG GTCACGTGTC CTTCTCACTC 
AACTGGGAAG GACCCAGCGT ATCGGTCGAT TCACTCGAAT GTTCGGCGGT ATCAGCATTA 
CAGTGGTCTA GACGCGCACT CCTGAGCGGA CAGTGCGATG TGGCACTTGC TGGTGCTGTC 
AACGTTCTGA CACAGCCGCA AATGTTCATC GCTATGGACA AAGAAGGTAT CCTCTCTCGT 
ACTGGAACGA ATGCTACGTT CTCCTCCAAA CTCGATGGAA AGACGAGAGG CGACGGATGC 
GGTGTTCTGC TGTTGAAGCG ACGCTCAACA GCACTTCGCG ATGGCGACAA GATCCTCGCC 
ACTCTGCCAG CTGCCAGGAC CACTTACCAT GGTAGTGCCC ATGAGAGTGA GGAAGCTAGC 
ACCAACCAGT CACGCTTTCT GGCTCGAGTC ATGTCCGAAG CAAGTGTTCG TGCATCCGAT 
CTCATACACA TCGAAGCAAG CGGATTCCAT ACACAACAAG CAGAGGCCGC CGAGTTCGAA 
TCTTTCAGCC GTCTGCTACC TCAATCCCAA GGTGATCGTT CATCCCAGGT CAGAGATAGG 
ATCTCGGTCG CGAGTTCTCG GCCGAACATT GGTGCAGGCG AAGCGGTGTC TGCCATGGCG 
TCGGTGATTA AGGCTGTGCT CATGTTTCAG CAAGGCAGTG TGCCACGACA GATCTCCATC 
TCGGATCCGT CCGAGCTTCA GCCCAGCATT ACTGCGATCT GCGCCGGTTC ATCTTTGTTT 
GTTCCAACGC AAGCGCGAAG ACTGCCGTCC GATAGAGACC ACGAAGGCCG ACACGTTATC 
CTGGTCAACA GTCTCGCGAG CACAAATTGC CATGGAGTCG TGCTGGTCGG TGCACCAACG 
ACTGACGACG TTGCACCACT GCAGATTCGT TCCAAAGCAC TTGACGCCAC TTTTACACGC 
ACAGAAGGGA CCTGGGTCTT TCTGCTCTCC GCAAAGACGA GGGAGTCAGC AGAGATGCTA 
AGAAAGGCCC TGATCGACTA TCTTCGCCGT GACGTGAATC TTGCCGATTT GAGCTACACT 
CTTACTTGTC GCAGGACGCA TCACCCGTTC CGTCTGAGCG TGGTAGCCTC GGACAAAGAT 
GAGCTGGTTC GGTCTCTGCG TACCGCTGAG TTCATCGAAG CTAAAGCGCT GGCCGATCTT 
CCGGCGTTTG GCCTCGTCCT TCGCCATCCT GAGCGGTCAG CTTGTCAAAA CATGGAAGGA 
TCGTTGGACC TTATCGTTGG ACTACGAGAT CGCTTCGAAG AACTGACATC AGTTATGGGT 
TACTCCGAAA TTCCAGCCGA TACAGAGCGG AAGCTGAATT TGAAGCAGGT TTGTCTAGCT 
TTGCTTCTCG ACGACTGCGG CGTCCACCCA TTGTCTTTCC ATGGCAGCTA TGATCTCATG 
CAGCTCTTGC AACGCTCGTT CCCAGCAGCG AAAGAAAGCG ATCTGTCCAA ACGCTTTACG 
TGGATTCACA TCGGAAGCCT GATCAGCATA TCCACCACCG AAGCCGCGGA ACAAGCAAAG 
CCTGCCGGTT CCGTGGCCGA CGGTCAGAAC CAGCAAAGGT CATTGCTCTC CACGCTTGGG 
TCTCTGCATC AAAAAGGTCA CTGCATTCGT TGGATCGAGT ACTTCCGTCC TCTTCTGGCG 
AAACTTAACT TTATCGAGAC GCTGCCCACA TATCTTTTCA ACCTTCAGCA GCACTGGATG 
GAGTATCACG ACCGCGATCT TCTTCCGCGA TCGGCTTCCA AGGAGGGTCA AGGTGGACAG 
AGGATCCGGC ACATCAGTGA TGGCGTTCTT TACTCGCCTT CAAGCCAAGT CGAAGCTTGG 
GTATCGCCGT CGCAGCCTTT GCTAAGCGAG CAAACGGCAG CAGACGACAG ATTGAGGACG 
TACAGCTCTA GCCTTTTCGA TGAAGCTGCT GCAGCCTTGC TGAGCGAAGC TAGCCCGGGA 
GCTCTGATCG CGGAGCTGGT AGTGGAAGCG ATCAAGGAAG CTTGCGAATC GATTGAGGGA 
ACCGTCGAAG CCTATGAGCA TCGCCCTGTT CGGCTTTATG AATTGTTCTT GGCCGAAGTG 
GTCAAGCAAT GGCCAGCCAC TGGCAAGGTC AAGCTTTCAG TGAAGTCGGT GCAGCAGACA 
GGGAATCGAA GCGAAGGGAC AGTTCAAATC TCTTTTGACG CTACTACTTC GCCTGTCGGC 
GAATGCCGAT ATGAATGGAT CACGCAGACA AACATGCATC AGAGATGGTG GAAGCTGCAG 
AAGCTGCTCG CAGAGAAGAT TTCGACGATC AAAGACAAAG GCGAGCTCTT CTCTCCAAAG 
CTCATCTATA AAACCCTCGG AGTTGACCCC ACGACGACTA CGATGTCGAT CCACAGCGCG 
TACCTCGACT GTCAATCGAG CGAAGCGGTC GTTTGCAACA CCATTCGTAC GCACACATCT 
GCTGTTGGAC AGATTGCACT GCCGCAGCTT CTGTCCAGTC TCGAGGAGAT TGCACGCTGG 
TTTGTTGGCG ACAAACCAAG CTCGGCTGAT GGCAAACTCA CGTTCATCGG CGTGGACGAG 
CTGCTCATTT GTGCCGATTG GCTTGATAGC CTGACTCGAC CAGCAGGTGC GTCTGTCAGC 
TACACGGCCT ACATCTCGCA GAGTCACACA GATGGTTCAT TGCGTGCTTC TACAGATGGG 
CTGGAGCTTG ACATTTTGAT CCTCGATGGC ATGCACAAGG TCGTTGGGGA GCTCAGCCGT 
CTTCGACTCG CAAAGTGCAA CAGCACTGCG AAGAAGACGA CGTCGAAGTG TGCTTCAGAG 
TCGACTCTGG CGCTTTCCAG TGGTGTCAAG ACAGTGTCAG GGTCATTTCC GGTGCAAGAG 
CCTGTTGAGC AACACAAGGA GCCCCGATCG ACCTCTGCAA AGCCTGTAGC AGGGAGACAA 
TCGAGCCGAG CAATGGGACT GCACGCCAAA GTGCTCGATG TGCTTGCTTC CGAACTGGGC 
GTTGCGGTCG AGGAGATGAA GCCCACCGTT AAGTTTGCCG ATCTCGGACT GGACAGTCTC 
ATGTCACTAG TTTGCATCTC TACTCTAGAG ACTATGAACC TTGGGCTTGA CATCCCGCAG 
TCGTTGTTTA TGGAGTGCGA CAGCCCTGCA GAGCTGTTGC AGTTTATCCG GGAGCAGGTC 
AGTGACGGAA CTGACGTTGG CCAGTTGGGG CCAGACGAGT CAACAAACGC AACGGAGCAA 
GTTCAGGGAA GCGATGCTGT GGTTCAATTG TCGCCGAGCG CCAACGCAGC TGAGACTCAG 
CTGACTGCGA CGACGGGCCA GGGCAGCTCT GCGGTGGAAG AGGCGATGAA GGCGATCCGA 
TGCACCGTTG AGACGGAGCT CGGTGTTGAC CTAGGAAGCA TCGATGAGGA TGCCAACTTG 
GCCGATCTGG GCATGGACTC GCTCATGTCG CTGCTCGTGC TTGGAAATTT GTCGGGAACG 
CTGCCGATCG AGCTGCCTTC GTCGCTTTTT ATGGATTGTT CATCGCTTGG CGAGATCCGG 
TCTTTTATGA CGAGCCAACT CGGAGCTGCA CGCGACTGTG CTCGCTCGGA CACTGTGGAC 
CAGCGAGATT CGAACTCGCA AATCGCGTTC TTTCTTCCAC CTGTCAAGAA ACCAATCCTC 
ATCCGCGAAG GGAGCAGCTC TGGCATGGAC CCGCCGTTGT TCCTTCTGCC CGAGGGAGCA 
GGCATGGCGA CGGTCTACCA ACAGTTCCCA GGCATTGATC GAGCGATCTA CTCGATCAAT 
TCGCCGTTTC TAGCCGACAC CTCGGCATGG ACGGGCGGGA TCGCCCAGAT TGCGCGGTAC 
TATCTCGAGA GTATCAAGCT AATCCAACCG GTGGGTCCGT GGCTGGTGGG AGGGTGGAGT 
TTCGGTGGAA TGGCTGCGTT TGAGATCGCG CGTTTGCTCG CTGACTGTGG TGGAGACAAG 
GACCGAGTCG CTGGACTATT CATTCTCGAC TCGCCCAGTC CCGAATATCC TCCTCTACCG 
ATGAGCATTC TCGACTGGAT CAACAGCGCT CCCGAAGTGC GCGACATGGC GCCGCCCACC 
ATGTCTGCCA AGCTCATTGC TCACTTCAGG GCTACTGTCG ATAGCCTCGT TGGCTGGGAG 
CCGACGACGA TGCGTGCCGC CGACGAGGCG AGCAAACCGC TACCGAAAAC GTGGTACATT 
GTAGCGGAAC AAGCGCTGCC TGGCAAGATG GAGGATGTCA AAGAGCTCAA TGAAACCGTC 
AGGTGGCTCT TCCGATCGAA GCGCGCAGAG AAGAGTGGCG CAGACGGCTG GCAGCACTTG 
ATCCCACACC ATCTCACAGT GATCGGCGTG GCTGAAGCAA ACCACTTCAC CATGGTCAGA 
CGTCCTAACG TCGGTCAGGT CGCACACATC TTGCAGAACG CCTGTCGGAA TGCCTTGCTC 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis.
Nature444(7115)97-101(2006 Nov)
Kämper J,Kahmann R,Bölker M,Ma LJ,Brefort T,Saville BJ,Banuett F,Kronstad JW,Gold SE,Müller O,Perlin MH,Wösten HA,de Vries R,Ruiz-Herrera J,Reynaga-Peña CG,Snetselaar K,McCann M,Pérez-Martín J,Feldbrügge M,Basse CW,Steinberg G,Ibeas JI,Holloman W,Guzman P,Farman M,Stajich JE,Sentandreu R,González-Prieto JM,Kennell JC,Molina L,Schirawski J,Mendoza-Mendoza A,Greilinger D,Münch K,Rössel N,Scherer M,Vranes M,Ladendorf O,Vincon V,Fuchs U,Sandrock B,Meng S,Ho EC,Cahill MJ,Boyce KJ,Klose J,Klosterman SJ,Deelstra HJ,Ortiz-Castellanos L,Li W,Sanchez-Alonso P,Schreier PH,Häuser-Hahn I,Vaupel M,Koopmann E,Friedrich G,Voss H,Schlüter T,Margolis J,Platt D,Swimmer C,Gnirke A,Chen F,Vysotskaia V,Mannhaupt G,Güldener U,Münsterkötter M,Haase D,Oesterheld M,Mewes HW,Mauceli EW,DeCaprio D,Wade CM,Butler J,Young S,Jaffe DB,Calvo S,Nusbaum C,Galagan J,Birren BW

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UMAG_06414 cDNA ORF clone, Ustilago maydis 521

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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