ASTL cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASTL
Entrez Gene ID	100033191
Full Name	astacin like metalloendopeptidase
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007476539.2	XP_007476601.1	astacin-like metalloendopeptidase isoform X3
XM_007476538.1	XP_007476600.1	astacin-like metalloendopeptidase isoform X2
XM_007476537.1	XP_007476599.1	astacin-like metalloendopeptidase isoform X1

Pathways from BioSystems
Gene Ontology

The following ASTL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASTL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc06697	XM_007476537.1 Latest version!	Monodelphis domestica astacin-like metallo-endopeptidase (M12 family) (ASTL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMc06698	XM_007476538.1 Latest version!	Monodelphis domestica astacin-like metallo-endopeptidase (M12 family) (ASTL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMc06699	XM_007476539.2 Latest version!	Monodelphis domestica astacin-like metallo-endopeptidase (M12 family) (ASTL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASTL ( XM_007476537.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007476599.1 Monodelphis domestica astacin-like metallo-endopeptidase (M12 family) (ASTL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc06697
Clone ID Related Accession (Same CDS sequence)	XM_007476537.1
Accession Version	XM_007476537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	astacin-like metalloendopeptidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGG TCTTGGACCT CTGGTCCTTG GTGCTGGGTC TGCTCACACT TGTGGCAGGT 
TTGGATCCTA CAAGACCTCC AGAAGAGAAT GCTACCAGTT TCATGGAGCA TGTCACCCCT 
GAAGGGACCC AGTCCACGTG GGATGGGGAT ATCCCTGCTA TTAACCAAGG TCTCATTCCT 
GAAGAATCCC CAGAGAGCAG CTTCCTAGTC GAAGGTGACA TCATCAAGGC AAGACCCTTC 
CAGCTGTTTT CTGCTGCCAA TTTCAAATGG CCCAAGAAGA ATGGAATTGT AGAGATCCCA 
TATATTTTCT CCAGCAAATA TGATCAGCCC AGCCAGGAAG TCATTCTGAA GGCTTTTGCT 
GACTTTGAGC ACCTCACTTG TATTAGGTTT GTTCCTCGGA CAAAGCAGAA GGACTTTGTT 
TCCATCATTC CTATGTCTGG GTGCTTCTCC AGTGTGGGAC ACAGTGGCGG GATGCAAGTG 
ACCTCTCTGG CCCCCTTCTG CCTCCAGAAA GGCAAAGGCA TTGTGCTCCA CGAGCTCATG 
CATGTGCTGG GATTCTGGCA TGAGCACTCC CGGGCTGATC GAGACCGCTA TATTCACGTG 
TCCTGGAAAG AGATCCGGCC AGGCTTTGAA ATCAACTTCA TCAAATCTCA GAACACCAAC 
ATGCTGGTGC CTTATGACTA CACGTCAGTG ATGCACTATG GGAGATATGC TTTTAGCAAG 
AACGGTAACA CCACCATTGT GCCACTGGCT GGACCGGACA TCCCCATTGG TCAGCGATGG 
AACCTAAGCA CTTCAGACAT CATTCGGGTC AATAGACTTT ATGAATGCAG CCAGACCATG 
AAAGATCCAG GGTTCCACAG TGAGAATAAT TGGAGTCACC AAAACATGGG GGTTATGGAC 
TTCATTGTCC CAGATGAAAT GAAGCTATCT CCTACTGAAT CCTCTTTGGA GACAGACTCA 
TCCCTGAAAG CGGTGAGCCA CCCAGAGGCA GGAAATAGGG TGTCTCCTGG TGGTTCTGAC 
TCTGCCTACA GTGGCTTTCC TGGGGCGAAA AAGGTGGCAA AAAATATTTG GCCCACTTGG 
ATTACCTCAG AATCTTCTCC TGAAGCTGCC CCGCTGCCAG ATTTAGTCTC CCCCTCTAAA 
GAAGTCCCTC AGGAGTCCAT GAAGAATACA TCAATTAGCT TAAATAACAC AGAAACAACT 
CAGCTTCTGA ATGAACCAGT ATCAGGCACT TCTGTCCATC TCCTCAAAAT TCTTCCCAGA 
GGGACAATGG CCAGTTTTCC AGAATCCAAG CATCTGCCTG AGATACATAA CAGTATCCTA 
GAGGAAATGG GAGAGGCTGG TGGGCTGTCA AGCACCCCGG TACCTGGCAG GGGGGCCTGG 
CCTGAAGGCA CAGTTCTCTT GCCTTCTGGG AATGCCTCCT TCTCCCTGGA ATTCCAGAGC 
CCCAGCCCAG ATTGGAAAGC TAGACTCAGG AACATTGTAG CAGACATTCA CGCCTCTCAC 
CCCAGGTTTG GCAGCATCCC GGGGCAACCT CATGGATGCC AACTGTGTGG CCAGAAGGAA 
ATAGACTGGG CTTCTACTGC TGTAGATGTA GCCCTGCCAA GTGGAGAGGA AGGGTTGCTT 
TGGCGCCTGC TGAAGGCCTT AACCATGGTA TCAGCCTCTA GGAATTTGCC AACCATCAGA 
GCACGGGGTC CAGGGACTAG GAATCAAGTC TTTGCTGCAG AACTGAATGG AAATAATGCT 
CCATCTAAGA CTATGGGCAC GGTATTCCCT GCCCGTGGGA CACACAACCA AGAGGCCCCT 
GAAGAGCTAG CCCAGACCCT GGGAGGAAGG AAGGAGGAAG GAAGAACAGA AGCAGATTCC 
AAAAAGAGAG AAAAACTTCT AAAACTGTTA GCCTTTGGAG ATGCAACCAG TGTTCCATAT 
GTACCAGCCA GGCATAAACC CTCCCCAAGC ATTGGCCAGA GTAGTACCTG GCCCAGATGG 
AGATCTAGAC CTAGAAACCA GCTGAGCCAA GAGAAAGCTA TTCTACAACA GCCCAGGAGG 
GACAACTCCT CCGGGGGTCA TGTGGCAACA GATCTCTCTG GGGTGTTCTC TCCATTCAGC 
AGGTCCAACC TAGGTTGGGC ATTAAGCAGG TCTCTGTCTC CGAGAGGAGG AATTCCTGCT 
CTGAGGAGGG TCCCTTGGGC AGGATCAGGA AACCCAGTAA CTGGAAACTT GGGAGTAGCC 
GGTAACTTTA GAGAGGAATT TGGAAGTGCC CCTTACGCTC TCCCAGTGGA AAAGGCTCCA 
TTTATTTCTC CAGAAAAGAA GCTTCCTTCT CTAGAGAATC AGGCCACCGA AGGTGAATGG 
GGGCTTCTAT CCACCCTGCC AAGGACCATC AGTCAGACTC CTTCTAGCCC CATGGCTCCT 
TCCCTGTTAT TAAAGCCTCA GACTAATAAG ATTCTAGCTC AGATAAAGTC ATCTCTGCTG 
GCTGCCAGCA CCCAGACCTC TTACCTGACC CATCCGATGG AAGATATGAC AAGTAACCAT 
TTTATTTTTC CTGTTCCTTC AAGAAAAAAA CAATACCTGG GAACAGAGAC TCCAGATGAG 
ACTTTTCAGA GGTCTGTAGG AGCTCACTCT GAGGTCAAGA CCACCACAGT GGATGGAAAG 
GAAGTGAGAC AAGCTGCTTC TCCTTCCACT GTGGGGTCAG CATTTCCTGA AAGGGAAGTG 
GCCAGCCCTG CTGTCTCTAT AAATTCAATC TGGTCCCTGG AGGATTCTGG GAACCAAAAG 
CCACATCCTC CTGGAAGAAT CAAACAGGGG GGCCTGAGAG TAGCTGGAAA GAAATGGATT 
TCTTCCTTTG CCCCTCTCAG GAGATGTCCC TCTGGAGGGA TCCAGCTTTT CCTAGGGGGA 
TTGATGATTC CCCTGCTTCC TGTGGAACCT GAGAGAATCC CAAGGGAATA TAAATCTAAG 
GGAACTTTGA CTCTCCTGGC TCTAGGGGTG GAGAGAAAGG TACCATCCTT TCTTTCCTGG 
ATCTCCAACT CCAGCAATGT CACACTGGGG CATCTCATGT CTCCTCCCCA ATTTCTAGAG 
GATCCTCAGA GCCAAACTTT TTTCCTTGCT GAGGCCAAAT CAGAAATCCC CAGGCACCAG 
AGGGTTCTTG ATCCCAAGTT TGCTGAGGCA GGAATTGTGC TTTTAAAAAG CCCATCTGGC 
CTCTTGAGTC TGGTGCCCCT TTGGGAGTGC TGGAGCCTCA GCAAAGTCAG AGAATCAGCC 
TATTTTCCAG AACCAGAAGC CCTGGAAAGG AAATATCGCT CTGCCTTAGT TGAGAATCCC 
TCCTCAGCTA TGGAAGCCAT GGACCTGCCC TCACAGGCCC TTAAGACTCA AACCCCTTTC 
CTGGACCTAT TACATGAAGA CAAAGGGAAG GGCCAATTTG GAATTAGGTC TGTTCCTTTG 
ATTCAGACAA AGGCAGGAAT GGCCCATTCA GTGAAGGATG AAGTAACACA GATCCTGGAG 
GAGGAAAATA GAACCTCAAA GGGCCTGGGA GGCAGAGACC CTAAACTCTC TCCCTTGAGG 
CTGGGCTTTC ATGGTGATAT TCTGCCCTCG AGTAAGCCTT CCTGGAGGCC TAATACCTTC 
CAGGGGATAT CTGATGTCAA GAGTCTGCTA GGTCTGGAGA CTCAAACCAG TAAATGGGCC 
AGAGGGACAC TTTCTCTCTT GGCTACCTCA AGAAGGGGGA CAGAGTCAGT GAGAGGTACT 
TGGACAACAA CCCTCCATGG TGAGCCCAAG TTGAATGAAG AGCTCCCACA GGGCAGATTC 
AAAATTCAAG CTGGCCCCAG ATTGCCCTCG CTTTCCCTGC CTGAGCCTGT GATGGAGCCC 
CAGGTTACTG ACATCCCTTT TCTGTGGGAA CCTCACACCA AGAAGGGCAG AGGAGAGAGA 
AAGCCACATT CCCTAAGCCC TTCGAAGGCT GGACATGGCA ACATGCCTTT GACAGACTAC 
AACTCTGGGG AGCCATTTAG TGAGAACATA CTCCACCACC ATATGGCTTG GGTGGGGAGG 
CAGGGGGTCC AAGGAAACCA TCTGTCTCTG CAGAACCAAG CTTCTTCACA AATAATCCCC 
AAAGCGGTTC TGATTAGCCC AGTGTTGTAT GGAGCCAGAT GGATTCGGTT TTGGTACAGT 
CCCCTGCAGC CTGGCATGGG AACCATTAGT GTCTATGTCT ACTCTCGGGC TCAATTTGGG 
TGGCATCTAC TCTGGTCCAG TGTGGGGCAT AGAAACGCAT CCTGGAGCTG GGTGGAACTG 
AAGCTTTGGC CATCACGGAA GATACAGGTG ATGGTAGAGG GAGTTCAAGG CCCAGAAGAT 
GGTGCTCATG TGAGCATTGA TAACCTTTCT GTCTCTAGCC ACCAATGGCC AAAGGGGGAA 
ACCAAGCTTC TTTGCCATTC TTTTCAAGAT TCCCTAAATA AAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007476599.1
CDS	77..4504
Translation	MRLVLDLWSLVLGLLTLVAGLDPTRPPEENATSFMEHVTPEGTQSTWDGDIPAINQGLIPEESPESSFLVEGDIIKARPFQLFSAANFKWPKKNGIVEIPYIFSSKYDQPSQEVILKAFADFEHLTCIRFVPRTKQKDFVSIIPMSGCFSSVGHSGGMQVTSLAPFCLQKGKGIVLHELMHVLGFWHEHSRADRDRYIHVSWKEIRPGFEINFIKSQNTNMLVPYDYTSVMHYGRYAFSKNGNTTIVPLAGPDIPIGQRWNLSTSDIIRVNRLYECSQTMKDPGFHSENNWSHQNMGVMDFIVPDEMKLSPTESSLETDSSLKAVSHPEAGNRVSPGGSDSAYSGFPGAKKVAKNIWPTWITSESSPEAAPLPDLVSPSKEVPQESMKNTSISLNNTETTQLLNEPVSGTSVHLLKILPRGTMASFPESKHLPEIHNSILEEMGEAGGLSSTPVPGRGAWPEGTVLLPSGNASFSLEFQSPSPDWKARLRNIVADIHASHPRFGSIPGQPHGCQLCGQKEIDWASTAVDVALPSGEEGLLWRLLKALTMVSASRNLPTIRARGPGTRNQVFAAELNGNNAPSKTMGTVFPARGTHNQEAPEELAQTLGGRKEEGRTEADSKKREKLLKLLAFGDATSVPYVPARHKPSPSIGQSSTWPRWRSRPRNQLSQEKAILQQPRRDNSSGGHVATDLSGVFSPFSRSNLGWALSRSLSPRGGIPALRRVPWAGSGNPVTGNLGVAGNFREEFGSAPYALPVEKAPFISPEKKLPSLENQATEGEWGLLSTLPRTISQTPSSPMAPSLLLKPQTNKILAQIKSSLLAASTQTSYLTHPMEDMTSNHFIFPVPSRKKQYLGTETPDETFQRSVGAHSEVKTTTVDGKEVRQAASPSTVGSAFPEREVASPAVSINSIWSLEDSGNQKPHPPGRIKQGGLRVAGKKWISSFAPLRRCPSGGIQLFLGGLMIPLLPVEPERIPREYKSKGTLTLLALGVERKVPSFLSWISNSSNVTLGHLMSPPQFLEDPQSQTFFLAEAKSEIPRHQRVLDPKFAEAGIVLLKSPSGLLSLVPLWECWSLSKVRESAYFPEPEALERKYRSALVENPSSAMEAMDLPSQALKTQTPFLDLLHEDKGKGQFGIRSVPLIQTKAGMAHSVKDEVTQILEEENRTSKGLGGRDPKLSPLRLGFHGDILPSSKPSWRPNTFQGISDVKSLLGLETQTSKWARGTLSLLATSRRGTESVRGTWTTTLHGEPKLNEELPQGRFKIQAGPRLPSLSLPEPVMEPQVTDIPFLWEPHTKKGRGERKPHSLSPSKAGHGNMPLTDYNSGEPFSENILHHHMAWVGRQGVQGNHLSLQNQASSQIIPKAVLISPVLYGARWIRFWYSPLQPGMGTISVYVYSRAQFGWHLLWSSVGHRNASWSWVELKLWPSRKIQVMVEGVQGPEDGAHVSIDNLSVSSHQWPKGETKLLCHSFQDSLNKT

Target ORF information:

RefSeq Version	XM_007476537.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica astacin-like metallo-endopeptidase (M12 family) (ASTL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007476537.1

ORF Insert Sequence:

ATGAGGCTGG TCTTGGACCT CTGGTCCTTG GTGCTGGGTC TGCTCACACT TGTGGCAGGT 
TTGGATCCTA CAAGACCTCC AGAAGAGAAT GCTACCAGTT TCATGGAGCA TGTCACCCCT 
GAAGGGACCC AGTCCACGTG GGATGGGGAT ATCCCTGCTA TTAACCAAGG TCTCATTCCT 
GAAGAATCCC CAGAGAGCAG CTTCCTAGTC GAAGGTGACA TCATCAAGGC AAGACCCTTC 
CAGCTGTTTT CTGCTGCCAA TTTCAAATGG CCCAAGAAGA ATGGAATTGT AGAGATCCCA 
TATATTTTCT CCAGCAAATA TGATCAGCCC AGCCAGGAAG TCATTCTGAA GGCTTTTGCT 
GACTTTGAGC ACCTCACTTG TATTAGGTTT GTTCCTCGGA CAAAGCAGAA GGACTTTGTT 
TCCATCATTC CTATGTCTGG GTGCTTCTCC AGTGTGGGAC ACAGTGGCGG GATGCAAGTG 
ACCTCTCTGG CCCCCTTCTG CCTCCAGAAA GGCAAAGGCA TTGTGCTCCA CGAGCTCATG 
CATGTGCTGG GATTCTGGCA TGAGCACTCC CGGGCTGATC GAGACCGCTA TATTCACGTG 
TCCTGGAAAG AGATCCGGCC AGGCTTTGAA ATCAACTTCA TCAAATCTCA GAACACCAAC 
ATGCTGGTGC CTTATGACTA CACGTCAGTG ATGCACTATG GGAGATATGC TTTTAGCAAG 
AACGGTAACA CCACCATTGT GCCACTGGCT GGACCGGACA TCCCCATTGG TCAGCGATGG 
AACCTAAGCA CTTCAGACAT CATTCGGGTC AATAGACTTT ATGAATGCAG CCAGACCATG 
AAAGATCCAG GGTTCCACAG TGAGAATAAT TGGAGTCACC AAAACATGGG GGTTATGGAC 
TTCATTGTCC CAGATGAAAT GAAGCTATCT CCTACTGAAT CCTCTTTGGA GACAGACTCA 
TCCCTGAAAG CGGTGAGCCA CCCAGAGGCA GGAAATAGGG TGTCTCCTGG TGGTTCTGAC 
TCTGCCTACA GTGGCTTTCC TGGGGCGAAA AAGGTGGCAA AAAATATTTG GCCCACTTGG 
ATTACCTCAG AATCTTCTCC TGAAGCTGCC CCGCTGCCAG ATTTAGTCTC CCCCTCTAAA 
GAAGTCCCTC AGGAGTCCAT GAAGAATACA TCAATTAGCT TAAATAACAC AGAAACAACT 
CAGCTTCTGA ATGAACCAGT ATCAGGCACT TCTGTCCATC TCCTCAAAAT TCTTCCCAGA 
GGGACAATGG CCAGTTTTCC AGAATCCAAG CATCTGCCTG AGATACATAA CAGTATCCTA 
GAGGAAATGG GAGAGGCTGG TGGGCTGTCA AGCACCCCGG TACCTGGCAG GGGGGCCTGG 
CCTGAAGGCA CAGTTCTCTT GCCTTCTGGG AATGCCTCCT TCTCCCTGGA ATTCCAGAGC 
CCCAGCCCAG ATTGGAAAGC TAGACTCAGG AACATTGTAG CAGACATTCA CGCCTCTCAC 
CCCAGGTTTG GCAGCATCCC GGGGCAACCT CATGGATGCC AACTGTGTGG CCAGAAGGAA 
ATAGACTGGG CTTCTACTGC TGTAGATGTA GCCCTGCCAA GTGGAGAGGA AGGGTTGCTT 
TGGCGCCTGC TGAAGGCCTT AACCATGGTA TCAGCCTCTA GGAATTTGCC AACCATCAGA 
GCACGGGGTC CAGGGACTAG GAATCAAGTC TTTGCTGCAG AACTGAATGG AAATAATGCT 
CCATCTAAGA CTATGGGCAC GGTATTCCCT GCCCGTGGGA CACACAACCA AGAGGCCCCT 
GAAGAGCTAG CCCAGACCCT GGGAGGAAGG AAGGAGGAAG GAAGAACAGA AGCAGATTCC 
AAAAAGAGAG AAAAACTTCT AAAACTGTTA GCCTTTGGAG ATGCAACCAG TGTTCCATAT 
GTACCAGCCA GGCATAAACC CTCCCCAAGC ATTGGCCAGA GTAGTACCTG GCCCAGATGG 
AGATCTAGAC CTAGAAACCA GCTGAGCCAA GAGAAAGCTA TTCTACAACA GCCCAGGAGG 
GACAACTCCT CCGGGGGTCA TGTGGCAACA GATCTCTCTG GGGTGTTCTC TCCATTCAGC 
AGGTCCAACC TAGGTTGGGC ATTAAGCAGG TCTCTGTCTC CGAGAGGAGG AATTCCTGCT 
CTGAGGAGGG TCCCTTGGGC AGGATCAGGA AACCCAGTAA CTGGAAACTT GGGAGTAGCC 
GGTAACTTTA GAGAGGAATT TGGAAGTGCC CCTTACGCTC TCCCAGTGGA AAAGGCTCCA 
TTTATTTCTC CAGAAAAGAA GCTTCCTTCT CTAGAGAATC AGGCCACCGA AGGTGAATGG 
GGGCTTCTAT CCACCCTGCC AAGGACCATC AGTCAGACTC CTTCTAGCCC CATGGCTCCT 
TCCCTGTTAT TAAAGCCTCA GACTAATAAG ATTCTAGCTC AGATAAAGTC ATCTCTGCTG 
GCTGCCAGCA CCCAGACCTC TTACCTGACC CATCCGATGG AAGATATGAC AAGTAACCAT 
TTTATTTTTC CTGTTCCTTC AAGAAAAAAA CAATACCTGG GAACAGAGAC TCCAGATGAG 
ACTTTTCAGA GGTCTGTAGG AGCTCACTCT GAGGTCAAGA CCACCACAGT GGATGGAAAG 
GAAGTGAGAC AAGCTGCTTC TCCTTCCACT GTGGGGTCAG CATTTCCTGA AAGGGAAGTG 
GCCAGCCCTG CTGTCTCTAT AAATTCAATC TGGTCCCTGG AGGATTCTGG GAACCAAAAG 
CCACATCCTC CTGGAAGAAT CAAACAGGGG GGCCTGAGAG TAGCTGGAAA GAAATGGATT 
TCTTCCTTTG CCCCTCTCAG GAGATGTCCC TCTGGAGGGA TCCAGCTTTT CCTAGGGGGA 
TTGATGATTC CCCTGCTTCC TGTGGAACCT GAGAGAATCC CAAGGGAATA TAAATCTAAG 
GGAACTTTGA CTCTCCTGGC TCTAGGGGTG GAGAGAAAGG TACCATCCTT TCTTTCCTGG 
ATCTCCAACT CCAGCAATGT CACACTGGGG CATCTCATGT CTCCTCCCCA ATTTCTAGAG 
GATCCTCAGA GCCAAACTTT TTTCCTTGCT GAGGCCAAAT CAGAAATCCC CAGGCACCAG 
AGGGTTCTTG ATCCCAAGTT TGCTGAGGCA GGAATTGTGC TTTTAAAAAG CCCATCTGGC 
CTCTTGAGTC TGGTGCCCCT TTGGGAGTGC TGGAGCCTCA GCAAAGTCAG AGAATCAGCC 
TATTTTCCAG AACCAGAAGC CCTGGAAAGG AAATATCGCT CTGCCTTAGT TGAGAATCCC 
TCCTCAGCTA TGGAAGCCAT GGACCTGCCC TCACAGGCCC TTAAGACTCA AACCCCTTTC 
CTGGACCTAT TACATGAAGA CAAAGGGAAG GGCCAATTTG GAATTAGGTC TGTTCCTTTG 
ATTCAGACAA AGGCAGGAAT GGCCCATTCA GTGAAGGATG AAGTAACACA GATCCTGGAG 
GAGGAAAATA GAACCTCAAA GGGCCTGGGA GGCAGAGACC CTAAACTCTC TCCCTTGAGG 
CTGGGCTTTC ATGGTGATAT TCTGCCCTCG AGTAAGCCTT CCTGGAGGCC TAATACCTTC 
CAGGGGATAT CTGATGTCAA GAGTCTGCTA GGTCTGGAGA CTCAAACCAG TAAATGGGCC 
AGAGGGACAC TTTCTCTCTT GGCTACCTCA AGAAGGGGGA CAGAGTCAGT GAGAGGTACT 
TGGACAACAA CCCTCCATGG TGAGCCCAAG TTGAATGAAG AGCTCCCACA GGGCAGATTC 
AAAATTCAAG CTGGCCCCAG ATTGCCCTCG CTTTCCCTGC CTGAGCCTGT GATGGAGCCC 
CAGGTTACTG ACATCCCTTT TCTGTGGGAA CCTCACACCA AGAAGGGCAG AGGAGAGAGA 
AAGCCACATT CCCTAAGCCC TTCGAAGGCT GGACATGGCA ACATGCCTTT GACAGACTAC 
AACTCTGGGG AGCCATTTAG TGAGAACATA CTCCACCACC ATATGGCTTG GGTGGGGAGG 
CAGGGGGTCC AAGGAAACCA TCTGTCTCTG CAGAACCAAG CTTCTTCACA AATAATCCCC 
AAAGCGGTTC TGATTAGCCC AGTGTTGTAT GGAGCCAGAT GGATTCGGTT TTGGTACAGT 
CCCCTGCAGC CTGGCATGGG AACCATTAGT GTCTATGTCT ACTCTCGGGC TCAATTTGGG 
TGGCATCTAC TCTGGTCCAG TGTGGGGCAT AGAAACGCAT CCTGGAGCTG GGTGGAACTG 
AAGCTTTGGC CATCACGGAA GATACAGGTG ATGGTAGAGG GAGTTCAAGG CCCAGAAGAT 
GGTGCTCATG TGAGCATTGA TAACCTTTCT GTCTCTAGCC ACCAATGGCC AAAGGGGGAA 
ACCAAGCTTC TTTGCCATTC TTTTCAAGAT TCCCTAAATA AAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASTL ( XM_007476538.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007476600.1 Monodelphis domestica astacin-like metallo-endopeptidase (M12 family) (ASTL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMc06698
Clone ID Related Accession (Same CDS sequence)	XM_007476538.1
Accession Version	XM_007476538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	astacin-like metalloendopeptidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATG TCACCCCTGA AGGGACCCAG TCCACGTGGG ATGGGGATAT CCCTGCTATT 
AACCAAGGTC TCATTCCTGA AGAATCCCCA GAGAGCAGCT TCCTAGTCGA AGGTGACATC 
ATCAAGGCAA GACCCTTCCA GCTGTTTTCT GCTGCCAATT TCAAATGGCC CAAGAAGAAT 
GGAATTGTAG AGATCCCATA TATTTTCTCC AGCAAATATG ATCAGCCCAG CCAGGAAGTC 
ATTCTGAAGG CTTTTGCTGA CTTTGAGCAC CTCACTTGTA TTAGGTTTGT TCCTCGGACA 
AAGCAGAAGG ACTTTGTTTC CATCATTCCT ATGTCTGGGT GCTTCTCCAG TGTGGGACAC 
AGTGGCGGGA TGCAAGTGAC CTCTCTGGCC CCCTTCTGCC TCCAGAAAGG CAAAGGCATT 
GTGCTCCACG AGCTCATGCA TGTGCTGGGA TTCTGGCATG AGCACTCCCG GGCTGATCGA 
GACCGCTATA TTCACGTGTC CTGGAAAGAG ATCCGGCCAG GCTTTGAAAT CAACTTCATC 
AAATCTCAGA ACACCAACAT GCTGGTGCCT TATGACTACA CGTCAGTGAT GCACTATGGG 
AGATATGCTT TTAGCAAGAA CGGTAACACC ACCATTGTGC CACTGGCTGG ACCGGACATC 
CCCATTGGTC AGCGATGGAA CCTAAGCACT TCAGACATCA TTCGGGTCAA TAGACTTTAT 
GAATGCAGCC AGACCATGAA AGATCCAGGG TTCCACAGTG AGAATAATTG GAGTCACCAA 
AACATGGGGG TTATGGACTT CATTGTCCCA GATGAAATGA AGCTATCTCC TACTGAATCC 
TCTTTGGAGA CAGACTCATC CCTGAAAGCG GTGAGCCACC CAGAGGCAGG AAATAGGGTG 
TCTCCTGGTG GTTCTGACTC TGCCTACAGT GGCTTTCCTG GGGCGAAAAA GGTGGCAAAA 
AATATTTGGC CCACTTGGAT TACCTCAGAA TCTTCTCCTG AAGCTGCCCC GCTGCCAGAT 
TTAGTCTCCC CCTCTAAAGA AGTCCCTCAG GAGTCCATGA AGAATACATC AATTAGCTTA 
AATAACACAG AAACAACTCA GCTTCTGAAT GAACCAGTAT CAGGCACTTC TGTCCATCTC 
CTCAAAATTC TTCCCAGAGG GACAATGGCC AGTTTTCCAG AATCCAAGCA TCTGCCTGAG 
ATACATAACA GTATCCTAGA GGAAATGGGA GAGGCTGGTG GGCTGTCAAG CACCCCGGTA 
CCTGGCAGGG GGGCCTGGCC TGAAGGCACA GTTCTCTTGC CTTCTGGGAA TGCCTCCTTC 
TCCCTGGAAT TCCAGAGCCC CAGCCCAGAT TGGAAAGCTA GACTCAGGAA CATTGTAGCA 
GACATTCACG CCTCTCACCC CAGGTTTGGC AGCATCCCGG GGCAACCTCA TGGATGCCAA 
CTGTGTGGCC AGAAGGAAAT AGACTGGGCT TCTACTGCTG TAGATGTAGC CCTGCCAAGT 
GGAGAGGAAG GGTTGCTTTG GCGCCTGCTG AAGGCCTTAA CCATGGTATC AGCCTCTAGG 
AATTTGCCAA CCATCAGAGC ACGGGGTCCA GGGACTAGGA ATCAAGTCTT TGCTGCAGAA 
CTGAATGGAA ATAATGCTCC ATCTAAGACT ATGGGCACGG TATTCCCTGC CCGTGGGACA 
CACAACCAAG AGGCCCCTGA AGAGCTAGCC CAGACCCTGG GAGGAAGGAA GGAGGAAGGA 
AGAACAGAAG CAGATTCCAA AAAGAGAGAA AAACTTCTAA AACTGTTAGC CTTTGGAGAT 
GCAACCAGTG TTCCATATGT ACCAGCCAGG CATAAACCCT CCCCAAGCAT TGGCCAGAGT 
AGTACCTGGC CCAGATGGAG ATCTAGACCT AGAAACCAGC TGAGCCAAGA GAAAGCTATT 
CTACAACAGC CCAGGAGGGA CAACTCCTCC GGGGGTCATG TGGCAACAGA TCTCTCTGGG 
GTGTTCTCTC CATTCAGCAG GTCCAACCTA GGTTGGGCAT TAAGCAGGTC TCTGTCTCCG 
AGAGGAGGAA TTCCTGCTCT GAGGAGGGTC CCTTGGGCAG GATCAGGAAA CCCAGTAACT 
GGAAACTTGG GAGTAGCCGG TAACTTTAGA GAGGAATTTG GAAGTGCCCC TTACGCTCTC 
CCAGTGGAAA AGGCTCCATT TATTTCTCCA GAAAAGAAGC TTCCTTCTCT AGAGAATCAG 
GCCACCGAAG GTGAATGGGG GCTTCTATCC ACCCTGCCAA GGACCATCAG TCAGACTCCT 
TCTAGCCCCA TGGCTCCTTC CCTGTTATTA AAGCCTCAGA CTAATAAGAT TCTAGCTCAG 
ATAAAGTCAT CTCTGCTGGC TGCCAGCACC CAGACCTCTT ACCTGACCCA TCCGATGGAA 
GATATGACAA GTAACCATTT TATTTTTCCT GTTCCTTCAA GAAAAAAACA ATACCTGGGA 
ACAGAGACTC CAGATGAGAC TTTTCAGAGG TCTGTAGGAG CTCACTCTGA GGTCAAGACC 
ACCACAGTGG ATGGAAAGGA AGTGAGACAA GCTGCTTCTC CTTCCACTGT GGGGTCAGCA 
TTTCCTGAAA GGGAAGTGGC CAGCCCTGCT GTCTCTATAA ATTCAATCTG GTCCCTGGAG 
GATTCTGGGA ACCAAAAGCC ACATCCTCCT GGAAGAATCA AACAGGGGGG CCTGAGAGTA 
GCTGGAAAGA AATGGATTTC TTCCTTTGCC CCTCTCAGGA GATGTCCCTC TGGAGGGATC 
CAGCTTTTCC TAGGGGGATT GATGATTCCC CTGCTTCCTG TGGAACCTGA GAGAATCCCA 
AGGGAATATA AATCTAAGGG AACTTTGACT CTCCTGGCTC TAGGGGTGGA GAGAAAGGTA 
CCATCCTTTC TTTCCTGGAT CTCCAACTCC AGCAATGTCA CACTGGGGCA TCTCATGTCT 
CCTCCCCAAT TTCTAGAGGA TCCTCAGAGC CAAACTTTTT TCCTTGCTGA GGCCAAATCA 
GAAATCCCCA GGCACCAGAG GGTTCTTGAT CCCAAGTTTG CTGAGGCAGG AATTGTGCTT 
TTAAAAAGCC CATCTGGCCT CTTGAGTCTG GTGCCCCTTT GGGAGTGCTG GAGCCTCAGC 
AAAGTCAGAG AATCAGCCTA TTTTCCAGAA CCAGAAGCCC TGGAAAGGAA ATATCGCTCT 
GCCTTAGTTG AGAATCCCTC CTCAGCTATG GAAGCCATGG ACCTGCCCTC ACAGGCCCTT 
AAGACTCAAA CCCCTTTCCT GGACCTATTA CATGAAGACA AAGGGAAGGG CCAATTTGGA 
ATTAGGTCTG TTCCTTTGAT TCAGACAAAG GCAGGAATGG CCCATTCAGT GAAGGATGAA 
GTAACACAGA TCCTGGAGGA GGAAAATAGA ACCTCAAAGG GCCTGGGAGG CAGAGACCCT 
AAACTCTCTC CCTTGAGGCT GGGCTTTCAT GGTGATATTC TGCCCTCGAG TAAGCCTTCC 
TGGAGGCCTA ATACCTTCCA GGGGATATCT GATGTCAAGA GTCTGCTAGG TCTGGAGACT 
CAAACCAGTA AATGGGCCAG AGGGACACTT TCTCTCTTGG CTACCTCAAG AAGGGGGACA 
GAGTCAGTGA GAGGTACTTG GACAACAACC CTCCATGGTG AGCCCAAGTT GAATGAAGAG 
CTCCCACAGG GCAGATTCAA AATTCAAGCT GGCCCCAGAT TGCCCTCGCT TTCCCTGCCT 
GAGCCTGTGA TGGAGCCCCA GGTTACTGAC ATCCCTTTTC TGTGGGAACC TCACACCAAG 
AAGGGCAGAG GAGAGAGAAA GCCACATTCC CTAAGCCCTT CGAAGGCTGG ACATGGCAAC 
ATGCCTTTGA CAGACTACAA CTCTGGGGAG CCATTTAGTG AGAACATACT CCACCACCAT 
ATGGCTTGGG TGGGGAGGCA GGGGGTCCAA GGAAACCATC TGTCTCTGCA GAACCAAGCT 
TCTTCACAAA TAATCCCCAA AGCGGTTCTG ATTAGCCCAG TGTTGTATGG AGCCAGATGG 
ATTCGGTTTT GGTACAGTCC CCTGCAGCCT GGCATGGGAA CCATTAGTGT CTATGTCTAC 
TCTCGGGCTC AATTTGGGTG GCATCTACTC TGGTCCAGTG TGGGGCATAG AAACGCATCC 
TGGAGCTGGG TGGAACTGAA GCTTTGGCCA TCACGGAAGA TACAGGTGAT GGTAGAGGGA 
GTTCAAGGCC CAGAAGATGG TGCTCATGTG AGCATTGATA ACCTTTCTGT CTCTAGCCAC 
CAATGGCCAA AGGGGGAAAC CAAGCTTCTT TGCCATTCTT TTCAAGATTC CCTAAATAAA 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007476600.1
CDS	126..4451
Translation	MEHVTPEGTQSTWDGDIPAINQGLIPEESPESSFLVEGDIIKARPFQLFSAANFKWPKKNGIVEIPYIFSSKYDQPSQEVILKAFADFEHLTCIRFVPRTKQKDFVSIIPMSGCFSSVGHSGGMQVTSLAPFCLQKGKGIVLHELMHVLGFWHEHSRADRDRYIHVSWKEIRPGFEINFIKSQNTNMLVPYDYTSVMHYGRYAFSKNGNTTIVPLAGPDIPIGQRWNLSTSDIIRVNRLYECSQTMKDPGFHSENNWSHQNMGVMDFIVPDEMKLSPTESSLETDSSLKAVSHPEAGNRVSPGGSDSAYSGFPGAKKVAKNIWPTWITSESSPEAAPLPDLVSPSKEVPQESMKNTSISLNNTETTQLLNEPVSGTSVHLLKILPRGTMASFPESKHLPEIHNSILEEMGEAGGLSSTPVPGRGAWPEGTVLLPSGNASFSLEFQSPSPDWKARLRNIVADIHASHPRFGSIPGQPHGCQLCGQKEIDWASTAVDVALPSGEEGLLWRLLKALTMVSASRNLPTIRARGPGTRNQVFAAELNGNNAPSKTMGTVFPARGTHNQEAPEELAQTLGGRKEEGRTEADSKKREKLLKLLAFGDATSVPYVPARHKPSPSIGQSSTWPRWRSRPRNQLSQEKAILQQPRRDNSSGGHVATDLSGVFSPFSRSNLGWALSRSLSPRGGIPALRRVPWAGSGNPVTGNLGVAGNFREEFGSAPYALPVEKAPFISPEKKLPSLENQATEGEWGLLSTLPRTISQTPSSPMAPSLLLKPQTNKILAQIKSSLLAASTQTSYLTHPMEDMTSNHFIFPVPSRKKQYLGTETPDETFQRSVGAHSEVKTTTVDGKEVRQAASPSTVGSAFPEREVASPAVSINSIWSLEDSGNQKPHPPGRIKQGGLRVAGKKWISSFAPLRRCPSGGIQLFLGGLMIPLLPVEPERIPREYKSKGTLTLLALGVERKVPSFLSWISNSSNVTLGHLMSPPQFLEDPQSQTFFLAEAKSEIPRHQRVLDPKFAEAGIVLLKSPSGLLSLVPLWECWSLSKVRESAYFPEPEALERKYRSALVENPSSAMEAMDLPSQALKTQTPFLDLLHEDKGKGQFGIRSVPLIQTKAGMAHSVKDEVTQILEEENRTSKGLGGRDPKLSPLRLGFHGDILPSSKPSWRPNTFQGISDVKSLLGLETQTSKWARGTLSLLATSRRGTESVRGTWTTTLHGEPKLNEELPQGRFKIQAGPRLPSLSLPEPVMEPQVTDIPFLWEPHTKKGRGERKPHSLSPSKAGHGNMPLTDYNSGEPFSENILHHHMAWVGRQGVQGNHLSLQNQASSQIIPKAVLISPVLYGARWIRFWYSPLQPGMGTISVYVYSRAQFGWHLLWSSVGHRNASWSWVELKLWPSRKIQVMVEGVQGPEDGAHVSIDNLSVSSHQWPKGETKLLCHSFQDSLNKT

Target ORF information:

RefSeq Version	XM_007476538.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica astacin-like metallo-endopeptidase (M12 family) (ASTL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007476538.1

ORF Insert Sequence:

ATGGAGCATG TCACCCCTGA AGGGACCCAG TCCACGTGGG ATGGGGATAT CCCTGCTATT 
AACCAAGGTC TCATTCCTGA AGAATCCCCA GAGAGCAGCT TCCTAGTCGA AGGTGACATC 
ATCAAGGCAA GACCCTTCCA GCTGTTTTCT GCTGCCAATT TCAAATGGCC CAAGAAGAAT 
GGAATTGTAG AGATCCCATA TATTTTCTCC AGCAAATATG ATCAGCCCAG CCAGGAAGTC 
ATTCTGAAGG CTTTTGCTGA CTTTGAGCAC CTCACTTGTA TTAGGTTTGT TCCTCGGACA 
AAGCAGAAGG ACTTTGTTTC CATCATTCCT ATGTCTGGGT GCTTCTCCAG TGTGGGACAC 
AGTGGCGGGA TGCAAGTGAC CTCTCTGGCC CCCTTCTGCC TCCAGAAAGG CAAAGGCATT 
GTGCTCCACG AGCTCATGCA TGTGCTGGGA TTCTGGCATG AGCACTCCCG GGCTGATCGA 
GACCGCTATA TTCACGTGTC CTGGAAAGAG ATCCGGCCAG GCTTTGAAAT CAACTTCATC 
AAATCTCAGA ACACCAACAT GCTGGTGCCT TATGACTACA CGTCAGTGAT GCACTATGGG 
AGATATGCTT TTAGCAAGAA CGGTAACACC ACCATTGTGC CACTGGCTGG ACCGGACATC 
CCCATTGGTC AGCGATGGAA CCTAAGCACT TCAGACATCA TTCGGGTCAA TAGACTTTAT 
GAATGCAGCC AGACCATGAA AGATCCAGGG TTCCACAGTG AGAATAATTG GAGTCACCAA 
AACATGGGGG TTATGGACTT CATTGTCCCA GATGAAATGA AGCTATCTCC TACTGAATCC 
TCTTTGGAGA CAGACTCATC CCTGAAAGCG GTGAGCCACC CAGAGGCAGG AAATAGGGTG 
TCTCCTGGTG GTTCTGACTC TGCCTACAGT GGCTTTCCTG GGGCGAAAAA GGTGGCAAAA 
AATATTTGGC CCACTTGGAT TACCTCAGAA TCTTCTCCTG AAGCTGCCCC GCTGCCAGAT 
TTAGTCTCCC CCTCTAAAGA AGTCCCTCAG GAGTCCATGA AGAATACATC AATTAGCTTA 
AATAACACAG AAACAACTCA GCTTCTGAAT GAACCAGTAT CAGGCACTTC TGTCCATCTC 
CTCAAAATTC TTCCCAGAGG GACAATGGCC AGTTTTCCAG AATCCAAGCA TCTGCCTGAG 
ATACATAACA GTATCCTAGA GGAAATGGGA GAGGCTGGTG GGCTGTCAAG CACCCCGGTA 
CCTGGCAGGG GGGCCTGGCC TGAAGGCACA GTTCTCTTGC CTTCTGGGAA TGCCTCCTTC 
TCCCTGGAAT TCCAGAGCCC CAGCCCAGAT TGGAAAGCTA GACTCAGGAA CATTGTAGCA 
GACATTCACG CCTCTCACCC CAGGTTTGGC AGCATCCCGG GGCAACCTCA TGGATGCCAA 
CTGTGTGGCC AGAAGGAAAT AGACTGGGCT TCTACTGCTG TAGATGTAGC CCTGCCAAGT 
GGAGAGGAAG GGTTGCTTTG GCGCCTGCTG AAGGCCTTAA CCATGGTATC AGCCTCTAGG 
AATTTGCCAA CCATCAGAGC ACGGGGTCCA GGGACTAGGA ATCAAGTCTT TGCTGCAGAA 
CTGAATGGAA ATAATGCTCC ATCTAAGACT ATGGGCACGG TATTCCCTGC CCGTGGGACA 
CACAACCAAG AGGCCCCTGA AGAGCTAGCC CAGACCCTGG GAGGAAGGAA GGAGGAAGGA 
AGAACAGAAG CAGATTCCAA AAAGAGAGAA AAACTTCTAA AACTGTTAGC CTTTGGAGAT 
GCAACCAGTG TTCCATATGT ACCAGCCAGG CATAAACCCT CCCCAAGCAT TGGCCAGAGT 
AGTACCTGGC CCAGATGGAG ATCTAGACCT AGAAACCAGC TGAGCCAAGA GAAAGCTATT 
CTACAACAGC CCAGGAGGGA CAACTCCTCC GGGGGTCATG TGGCAACAGA TCTCTCTGGG 
GTGTTCTCTC CATTCAGCAG GTCCAACCTA GGTTGGGCAT TAAGCAGGTC TCTGTCTCCG 
AGAGGAGGAA TTCCTGCTCT GAGGAGGGTC CCTTGGGCAG GATCAGGAAA CCCAGTAACT 
GGAAACTTGG GAGTAGCCGG TAACTTTAGA GAGGAATTTG GAAGTGCCCC TTACGCTCTC 
CCAGTGGAAA AGGCTCCATT TATTTCTCCA GAAAAGAAGC TTCCTTCTCT AGAGAATCAG 
GCCACCGAAG GTGAATGGGG GCTTCTATCC ACCCTGCCAA GGACCATCAG TCAGACTCCT 
TCTAGCCCCA TGGCTCCTTC CCTGTTATTA AAGCCTCAGA CTAATAAGAT TCTAGCTCAG 
ATAAAGTCAT CTCTGCTGGC TGCCAGCACC CAGACCTCTT ACCTGACCCA TCCGATGGAA 
GATATGACAA GTAACCATTT TATTTTTCCT GTTCCTTCAA GAAAAAAACA ATACCTGGGA 
ACAGAGACTC CAGATGAGAC TTTTCAGAGG TCTGTAGGAG CTCACTCTGA GGTCAAGACC 
ACCACAGTGG ATGGAAAGGA AGTGAGACAA GCTGCTTCTC CTTCCACTGT GGGGTCAGCA 
TTTCCTGAAA GGGAAGTGGC CAGCCCTGCT GTCTCTATAA ATTCAATCTG GTCCCTGGAG 
GATTCTGGGA ACCAAAAGCC ACATCCTCCT GGAAGAATCA AACAGGGGGG CCTGAGAGTA 
GCTGGAAAGA AATGGATTTC TTCCTTTGCC CCTCTCAGGA GATGTCCCTC TGGAGGGATC 
CAGCTTTTCC TAGGGGGATT GATGATTCCC CTGCTTCCTG TGGAACCTGA GAGAATCCCA 
AGGGAATATA AATCTAAGGG AACTTTGACT CTCCTGGCTC TAGGGGTGGA GAGAAAGGTA 
CCATCCTTTC TTTCCTGGAT CTCCAACTCC AGCAATGTCA CACTGGGGCA TCTCATGTCT 
CCTCCCCAAT TTCTAGAGGA TCCTCAGAGC CAAACTTTTT TCCTTGCTGA GGCCAAATCA 
GAAATCCCCA GGCACCAGAG GGTTCTTGAT CCCAAGTTTG CTGAGGCAGG AATTGTGCTT 
TTAAAAAGCC CATCTGGCCT CTTGAGTCTG GTGCCCCTTT GGGAGTGCTG GAGCCTCAGC 
AAAGTCAGAG AATCAGCCTA TTTTCCAGAA CCAGAAGCCC TGGAAAGGAA ATATCGCTCT 
GCCTTAGTTG AGAATCCCTC CTCAGCTATG GAAGCCATGG ACCTGCCCTC ACAGGCCCTT 
AAGACTCAAA CCCCTTTCCT GGACCTATTA CATGAAGACA AAGGGAAGGG CCAATTTGGA 
ATTAGGTCTG TTCCTTTGAT TCAGACAAAG GCAGGAATGG CCCATTCAGT GAAGGATGAA 
GTAACACAGA TCCTGGAGGA GGAAAATAGA ACCTCAAAGG GCCTGGGAGG CAGAGACCCT 
AAACTCTCTC CCTTGAGGCT GGGCTTTCAT GGTGATATTC TGCCCTCGAG TAAGCCTTCC 
TGGAGGCCTA ATACCTTCCA GGGGATATCT GATGTCAAGA GTCTGCTAGG TCTGGAGACT 
CAAACCAGTA AATGGGCCAG AGGGACACTT TCTCTCTTGG CTACCTCAAG AAGGGGGACA 
GAGTCAGTGA GAGGTACTTG GACAACAACC CTCCATGGTG AGCCCAAGTT GAATGAAGAG 
CTCCCACAGG GCAGATTCAA AATTCAAGCT GGCCCCAGAT TGCCCTCGCT TTCCCTGCCT 
GAGCCTGTGA TGGAGCCCCA GGTTACTGAC ATCCCTTTTC TGTGGGAACC TCACACCAAG 
AAGGGCAGAG GAGAGAGAAA GCCACATTCC CTAAGCCCTT CGAAGGCTGG ACATGGCAAC 
ATGCCTTTGA CAGACTACAA CTCTGGGGAG CCATTTAGTG AGAACATACT CCACCACCAT 
ATGGCTTGGG TGGGGAGGCA GGGGGTCCAA GGAAACCATC TGTCTCTGCA GAACCAAGCT 
TCTTCACAAA TAATCCCCAA AGCGGTTCTG ATTAGCCCAG TGTTGTATGG AGCCAGATGG 
ATTCGGTTTT GGTACAGTCC CCTGCAGCCT GGCATGGGAA CCATTAGTGT CTATGTCTAC 
TCTCGGGCTC AATTTGGGTG GCATCTACTC TGGTCCAGTG TGGGGCATAG AAACGCATCC 
TGGAGCTGGG TGGAACTGAA GCTTTGGCCA TCACGGAAGA TACAGGTGAT GGTAGAGGGA 
GTTCAAGGCC CAGAAGATGG TGCTCATGTG AGCATTGATA ACCTTTCTGT CTCTAGCCAC 
CAATGGCCAA AGGGGGAAAC CAAGCTTCTT TGCCATTCTT TTCAAGATTC CCTAAATAAA 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASTL ( XM_007476539.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007476601.1 Monodelphis domestica astacin-like metallo-endopeptidase (M12 family) (ASTL), transcript variant X3, mRNA.

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CloneID	OMc06699
Clone ID Related Accession (Same CDS sequence)	XM_007476539.2
Accession Version	XM_007476539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	astacin-like metalloendopeptidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007476539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGG TCTTGGACCT CTGGTCCTTG GTGCTGGGTC TGCTCACACT TGTGGCAGGT 
TTGGATCCTA CAAGACCTCC AGAAGAGAAT GCTACCAGTT TCATGGAGCA TGTCACCCCT 
GAAGGGACCC AGTCCACGTG GGATGGGGAT ATCCCTGCTA TTAACCAAGG TCTCATTCCT 
GAAGAATCCC CAGAGAGCAG CTTCCTAGTC GAAGGTGACA TCATCAAGGC AAGACCCTTC 
CAGCTGTTTT CTGCTGCCAA TTTCAAATGG CCCAAGAAGA ATGGAATTGT AGAGATCCCA 
TATATTTTCT CCAGCAAATA TGATCAGCCC AGCCAGGAAG TCATTCTGAA GGCTTTTGCT 
GACTTTGAGC ACCTCACTTG TATTAGGTTT GTTCCTCGGA CAAAGCAGAA GGACTTTGTT 
TCCATCATTC CTATGTCTGG GTGCTTCTCC AGTGTGGGAC ACAGTGGCGG GATGCAAGTG 
ACCTCTCTGG CCCCCTTCTG CCTCCAGAAA GGCAAAGGCA TTGTGCTCCA CGAGCTCATG 
CATGTGCTGG GATTCTGGCA TGAGCACTCC CGGGCTGATC GAGACCGCTA TATTCACGTG 
TCCTGGAAAG AGATCCGGCC AGGCTTTGAA ATCAACTTCA TCAAATCTCA GAACACCAAC 
ATGCTGGTGC CTTATGACTA CACGTCAGTG ATGCACTATG GGAGATATGC TTTTAGCAAG 
AACGGTAACA CCACCATTGT GCCACTGGCT GGACCGGACA TCCCCATTGG TCAGCGATGG 
AACCTAAGCA CTTCAGACAT CATTCGGGTC AATAGACTTT ATGAATGCAG CCAGACCATG 
AAAGATCCAG GGTTCCACAG TGAGAATAAT TGGAGTCACC AAAACATGGG GGTTATGGAC 
TTCATTGTCC CAGATGAAAT GAAGCTATCT CCTACTGAAT CCTCTTTGGA GACAGACTCA 
TCCCTGAAAG CGGTGAGCCA CCCAGAGGCA GGAAATAGGG TGTCTCCTGG TGGTTCTGAC 
TCTGCCTACA GTGGCTTTCC TGGGGCGAAA AAGGTGGCAA AAAATATTTG GCCCACTTGG 
ATTACCTCAG AATCTTCTCC TGAAGCTGCC CCGCTGCCAG ATTTAGTCTC CCCCTCTAAA 
GAAGTCCCTC AGGAGTCCAT GAAGAATACA TCAATTAGCT TAAATAACAC AGAAACAACT 
CAGCTTCTGA ATGAACCAGT ATCAGGCACT TCTGTCCATC TCCTCAAAAT TCTTCCCAGA 
GGGACAATGG CCAGTTTTCC AGAATCCAAG CATCTGCCTG AGATACATAA CAGTATCCTA 
GAGGAAATGG GAGAGGCTGG TGGGCTGTCA AGCACCCCGG TACCTGGCAG GGGGGCCTGG 
CCTGAAGGCA CAGTTCTCTT GCCTTCTGGG AATGCCTCCT TCTCCCTGGA ATTCCAGAGC 
CCCAGCCCAG ATTGGAAAGC TAGACTCAGG AACATTGTAG CAGACATTCA CGCCTCTCAC 
CCCAGGTTTG GCAGCATCCC GGGGCAACCT CATGGATGCC AACTGTGTGG CCAGAAGGAA 
ATAGACTGGG CTTCTACTGC TGTAGATGTA GCCCTGCCAA GTGGAGAGGA AGGGTTGCTT 
TGGCGCCTGC TGAAGGCCTT AACCATGGTA TCAGCCTCTA GGAATTTGCC AACCATCAGA 
GCACGGGGTC CAGGGACTAG GAATCAAGTC TTTGCTGCAG AACTGAATGG AAATAATGCT 
CCATCTAAGA CTATGGGCAC GGTATTCCCT GCCCGTGGGA CACACAACCA AGAGGCCCCT 
GAAGAGCTAG CCCAGACCCT GGGAGGAAGG AAGGAGGAAG GAAGAACAGA AGCAGATTCC 
AAAAAGAGAG AAAAACTTCT AAAACTGTTA GCCTTTGGAG ATGCAACCAG TGTTCCATAT 
GTACCAGCCA GGCATAAACC CTCCCCAAGC ATTGGCCAGA GTAGTACCTG GCCCAGATGG 
AGATCTAGAC CTAGAAACCA GCTGAGCCAA GAGAAAGCTA TTCTACAACA GCCCAGGAGG 
GACAACTCCT CCGGGGGTCA TGTGGCAACA GATCTCTCTG GGGTGTTCTC TCCATTCAGC 
AGGTCCAACC TAGGTTGGGC ATTAAGCAGG TCTCTGTCTC CGAGAGGAGG AATTCCTGCT 
CTGAGGAGGG TCCCTTGGGC AGGATCAGGA AACCCAGTAA CTGGAAACTT GGGAGTAGCC 
GGTAACTTTA GAGAGGAATT TGGAAGTGCC CCTTACGCTC TCCCAGTGGA AAAGGCTCCA 
TTTATTTCTC CAGAAAAGAA GCTTCCTTCT CTAGAGAATC AGGCCACCGA AGGTGAATGG 
GGGCTTCTAT CCACCCTGCC AAGGACCATC AGTCAGACTC CTTCTAGCCC CATGGCTCCT 
TCCCTGTTAT TAAAGCCTCA GACTAATAAG ATTCTAGCTC AGATAAAGTC ATCTCTGCTG 
GCTGCCAGCA CCCAGACCTC TTACCTGACC CATCCGATGG AAGATATGAC AAGTAACCAT 
TTTATTTTTC CTGTTCCTTC AAGAAAAAAA CAATACCTGG GAACAGAGAC TCCAGATGAG 
ACTTTTCAGA GGTCTGTAGG AGCTCACTCT GAGGTCAAGA CCACCACAGT GGATGGAAAG 
GAAGTGAGAC AAGCTGCTTC TCCTTCCACT GTGGGGTCAG CATTTCCTGA AAGGGAAGTG 
GCCAGCCCTG CTGTCTCTAT AAATTCAATC TGGTCCCTGG AGGATTCTGG GAACCAAAAG 
CCACATCCTC CTGGAAGAAT CAAACAGGGG GGCCTGAGAG TAGCTGGAAA GAAATGGATT 
TCTTCCTTTG CCCCTCTCAG GAGATGTCCC TCTGGAGGGA TCCAGCTTTT CCTAGGGGGA 
TTGATGATTC CCCTGCTTCC TGTGGAACCT GAGAGAATCC CAAGGGAATA TAAATCTAAG 
GGAACTTTGA CTCTCCTGGC TCTAGGGGTG GAGAGAAAGG TACCATCCTT TCTTTCCTGG 
ATCTCCAACT CCAGCAATGT CACACTGGGG CATCTCATGT CTCCTCCCCA ATTTCTAGAG 
GATCCTCAGA GCCAAACTTT TTTCCTTGCT GAGGCCAAAT CAGAAATCCC CAGGCACCAG 
AGGGTTCTTG ATCCCAAGTT TGCTGAGGCA GGAATTGTGC TTTTAAAAAG CCCATCTGGC 
CTCTTGAGTC TGGTGCCCCT TTGGGAGTGC TGGAGCCTCA GCAAAGTCAG AGAATCAGCC 
TATTTTCCAG AACCAGAAGC CCTGGAAAGG AAATATCGCT CTGCCTTAGT TGAGAATCCC 
TCCTCAGCTA TGGAAGCCAT GGACCTGCCC TCACAGGCCC TTAAGACTCA AACCCCTTTC 
CTGGACCTAT TACATGAAGA CAAAGGGAAG GGCCAATTTG GAATTAGGTC TGTTCCTTTG 
ATTCAGACAA AGGCAGGAAT GGCCCATTCA GTGAAGGATG AAGTAACACA GATCCTGGAG 
GAGGAAAATA GAACCTCAAA GGGCCTGGGA GGCAGAGACC CTAAACTCTC TCCCTTGAGG 
CTGGGCTTTC ATGGTGATAT TCTGCCCTCG AGTAAGCCTT CCTGGAGGCC TAATACCTTC 
CAGGGGATAT CTGATGTCAA GAGTCTGCTA GGTCTGGAGA CTCAAACCAG TAAATGGGCC 
AGAGGGACAC TTTCTCTCTT GGCTACCTCA AGAAGGGGGA CAGAGTCAGT GAGAGGTACT 
TGGACAACAA CCCTCCATGG TGAGCCCAAG TTGAATGAAG AGCTCCCACA GGGCAGATTC 
AAAATTCAAG CTGGCCCCAG ATTGCCCTCG CTTTCCCTGC CTGAGCCTGT GATGGAGCCC 
CAGGTTACTG ACATCCCTTT TCTGTGGGAA CCTCACACCA AGAAGGGCAG AGGAGAGAGA 
AAGCCACATT CCCTAAGCCC TTCGAAGGCT GGACATGGCA ACATGCCTTT GACAGACTAC 
AACTCTGGGG AGCCATTTAG TGAGAACATA CTCCACCACC ATATGGCTTG GGTGGGGAGG 
CAGGGGGTCC AAGGAAACCA TCTGTCTCTG CAGAACCAAG CTTCTTCACA AATAATCCCC 
AAAGCGGTTC TGATTAGCCC AGTGTTGTAT GGAGCCAGAT GGATTCGGTT TTGGTACAGT 
CCCCTGCAGC CTGGCATGGG TGATGGTAGA GGGAGTTCAA GGCCCAGAAG ATGGTGCTCA 
TGTGAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007476601.1
CDS	77..4288
Translation	MRLVLDLWSLVLGLLTLVAGLDPTRPPEENATSFMEHVTPEGTQSTWDGDIPAINQGLIPEESPESSFLVEGDIIKARPFQLFSAANFKWPKKNGIVEIPYIFSSKYDQPSQEVILKAFADFEHLTCIRFVPRTKQKDFVSIIPMSGCFSSVGHSGGMQVTSLAPFCLQKGKGIVLHELMHVLGFWHEHSRADRDRYIHVSWKEIRPGFEINFIKSQNTNMLVPYDYTSVMHYGRYAFSKNGNTTIVPLAGPDIPIGQRWNLSTSDIIRVNRLYECSQTMKDPGFHSENNWSHQNMGVMDFIVPDEMKLSPTESSLETDSSLKAVSHPEAGNRVSPGGSDSAYSGFPGAKKVAKNIWPTWITSESSPEAAPLPDLVSPSKEVPQESMKNTSISLNNTETTQLLNEPVSGTSVHLLKILPRGTMASFPESKHLPEIHNSILEEMGEAGGLSSTPVPGRGAWPEGTVLLPSGNASFSLEFQSPSPDWKARLRNIVADIHASHPRFGSIPGQPHGCQLCGQKEIDWASTAVDVALPSGEEGLLWRLLKALTMVSASRNLPTIRARGPGTRNQVFAAELNGNNAPSKTMGTVFPARGTHNQEAPEELAQTLGGRKEEGRTEADSKKREKLLKLLAFGDATSVPYVPARHKPSPSIGQSSTWPRWRSRPRNQLSQEKAILQQPRRDNSSGGHVATDLSGVFSPFSRSNLGWALSRSLSPRGGIPALRRVPWAGSGNPVTGNLGVAGNFREEFGSAPYALPVEKAPFISPEKKLPSLENQATEGEWGLLSTLPRTISQTPSSPMAPSLLLKPQTNKILAQIKSSLLAASTQTSYLTHPMEDMTSNHFIFPVPSRKKQYLGTETPDETFQRSVGAHSEVKTTTVDGKEVRQAASPSTVGSAFPEREVASPAVSINSIWSLEDSGNQKPHPPGRIKQGGLRVAGKKWISSFAPLRRCPSGGIQLFLGGLMIPLLPVEPERIPREYKSKGTLTLLALGVERKVPSFLSWISNSSNVTLGHLMSPPQFLEDPQSQTFFLAEAKSEIPRHQRVLDPKFAEAGIVLLKSPSGLLSLVPLWECWSLSKVRESAYFPEPEALERKYRSALVENPSSAMEAMDLPSQALKTQTPFLDLLHEDKGKGQFGIRSVPLIQTKAGMAHSVKDEVTQILEEENRTSKGLGGRDPKLSPLRLGFHGDILPSSKPSWRPNTFQGISDVKSLLGLETQTSKWARGTLSLLATSRRGTESVRGTWTTTLHGEPKLNEELPQGRFKIQAGPRLPSLSLPEPVMEPQVTDIPFLWEPHTKKGRGERKPHSLSPSKAGHGNMPLTDYNSGEPFSENILHHHMAWVGRQGVQGNHLSLQNQASSQIIPKAVLISPVLYGARWIRFWYSPLQPGMGDGRGSSRPRRWCSCEH

Target ORF information:

RefSeq Version	XM_007476539.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica astacin-like metallo-endopeptidase (M12 family) (ASTL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007476539.2

ORF Insert Sequence:

ATGAGGCTGG TCTTGGACCT CTGGTCCTTG GTGCTGGGTC TGCTCACACT TGTGGCAGGT 
TTGGATCCTA CAAGACCTCC AGAAGAGAAT GCTACCAGTT TCATGGAGCA TGTCACCCCT 
GAAGGGACCC AGTCCACGTG GGATGGGGAT ATCCCTGCTA TTAACCAAGG TCTCATTCCT 
GAAGAATCCC CAGAGAGCAG CTTCCTAGTC GAAGGTGACA TCATCAAGGC AAGACCCTTC 
CAGCTGTTTT CTGCTGCCAA TTTCAAATGG CCCAAGAAGA ATGGAATTGT AGAGATCCCA 
TATATTTTCT CCAGCAAATA TGATCAGCCC AGCCAGGAAG TCATTCTGAA GGCTTTTGCT 
GACTTTGAGC ACCTCACTTG TATTAGGTTT GTTCCTCGGA CAAAGCAGAA GGACTTTGTT 
TCCATCATTC CTATGTCTGG GTGCTTCTCC AGTGTGGGAC ACAGTGGCGG GATGCAAGTG 
ACCTCTCTGG CCCCCTTCTG CCTCCAGAAA GGCAAAGGCA TTGTGCTCCA CGAGCTCATG 
CATGTGCTGG GATTCTGGCA TGAGCACTCC CGGGCTGATC GAGACCGCTA TATTCACGTG 
TCCTGGAAAG AGATCCGGCC AGGCTTTGAA ATCAACTTCA TCAAATCTCA GAACACCAAC 
ATGCTGGTGC CTTATGACTA CACGTCAGTG ATGCACTATG GGAGATATGC TTTTAGCAAG 
AACGGTAACA CCACCATTGT GCCACTGGCT GGACCGGACA TCCCCATTGG TCAGCGATGG 
AACCTAAGCA CTTCAGACAT CATTCGGGTC AATAGACTTT ATGAATGCAG CCAGACCATG 
AAAGATCCAG GGTTCCACAG TGAGAATAAT TGGAGTCACC AAAACATGGG GGTTATGGAC 
TTCATTGTCC CAGATGAAAT GAAGCTATCT CCTACTGAAT CCTCTTTGGA GACAGACTCA 
TCCCTGAAAG CGGTGAGCCA CCCAGAGGCA GGAAATAGGG TGTCTCCTGG TGGTTCTGAC 
TCTGCCTACA GTGGCTTTCC TGGGGCGAAA AAGGTGGCAA AAAATATTTG GCCCACTTGG 
ATTACCTCAG AATCTTCTCC TGAAGCTGCC CCGCTGCCAG ATTTAGTCTC CCCCTCTAAA 
GAAGTCCCTC AGGAGTCCAT GAAGAATACA TCAATTAGCT TAAATAACAC AGAAACAACT 
CAGCTTCTGA ATGAACCAGT ATCAGGCACT TCTGTCCATC TCCTCAAAAT TCTTCCCAGA 
GGGACAATGG CCAGTTTTCC AGAATCCAAG CATCTGCCTG AGATACATAA CAGTATCCTA 
GAGGAAATGG GAGAGGCTGG TGGGCTGTCA AGCACCCCGG TACCTGGCAG GGGGGCCTGG 
CCTGAAGGCA CAGTTCTCTT GCCTTCTGGG AATGCCTCCT TCTCCCTGGA ATTCCAGAGC 
CCCAGCCCAG ATTGGAAAGC TAGACTCAGG AACATTGTAG CAGACATTCA CGCCTCTCAC 
CCCAGGTTTG GCAGCATCCC GGGGCAACCT CATGGATGCC AACTGTGTGG CCAGAAGGAA 
ATAGACTGGG CTTCTACTGC TGTAGATGTA GCCCTGCCAA GTGGAGAGGA AGGGTTGCTT 
TGGCGCCTGC TGAAGGCCTT AACCATGGTA TCAGCCTCTA GGAATTTGCC AACCATCAGA 
GCACGGGGTC CAGGGACTAG GAATCAAGTC TTTGCTGCAG AACTGAATGG AAATAATGCT 
CCATCTAAGA CTATGGGCAC GGTATTCCCT GCCCGTGGGA CACACAACCA AGAGGCCCCT 
GAAGAGCTAG CCCAGACCCT GGGAGGAAGG AAGGAGGAAG GAAGAACAGA AGCAGATTCC 
AAAAAGAGAG AAAAACTTCT AAAACTGTTA GCCTTTGGAG ATGCAACCAG TGTTCCATAT 
GTACCAGCCA GGCATAAACC CTCCCCAAGC ATTGGCCAGA GTAGTACCTG GCCCAGATGG 
AGATCTAGAC CTAGAAACCA GCTGAGCCAA GAGAAAGCTA TTCTACAACA GCCCAGGAGG 
GACAACTCCT CCGGGGGTCA TGTGGCAACA GATCTCTCTG GGGTGTTCTC TCCATTCAGC 
AGGTCCAACC TAGGTTGGGC ATTAAGCAGG TCTCTGTCTC CGAGAGGAGG AATTCCTGCT 
CTGAGGAGGG TCCCTTGGGC AGGATCAGGA AACCCAGTAA CTGGAAACTT GGGAGTAGCC 
GGTAACTTTA GAGAGGAATT TGGAAGTGCC CCTTACGCTC TCCCAGTGGA AAAGGCTCCA 
TTTATTTCTC CAGAAAAGAA GCTTCCTTCT CTAGAGAATC AGGCCACCGA AGGTGAATGG 
GGGCTTCTAT CCACCCTGCC AAGGACCATC AGTCAGACTC CTTCTAGCCC CATGGCTCCT 
TCCCTGTTAT TAAAGCCTCA GACTAATAAG ATTCTAGCTC AGATAAAGTC ATCTCTGCTG 
GCTGCCAGCA CCCAGACCTC TTACCTGACC CATCCGATGG AAGATATGAC AAGTAACCAT 
TTTATTTTTC CTGTTCCTTC AAGAAAAAAA CAATACCTGG GAACAGAGAC TCCAGATGAG 
ACTTTTCAGA GGTCTGTAGG AGCTCACTCT GAGGTCAAGA CCACCACAGT GGATGGAAAG 
GAAGTGAGAC AAGCTGCTTC TCCTTCCACT GTGGGGTCAG CATTTCCTGA AAGGGAAGTG 
GCCAGCCCTG CTGTCTCTAT AAATTCAATC TGGTCCCTGG AGGATTCTGG GAACCAAAAG 
CCACATCCTC CTGGAAGAAT CAAACAGGGG GGCCTGAGAG TAGCTGGAAA GAAATGGATT 
TCTTCCTTTG CCCCTCTCAG GAGATGTCCC TCTGGAGGGA TCCAGCTTTT CCTAGGGGGA 
TTGATGATTC CCCTGCTTCC TGTGGAACCT GAGAGAATCC CAAGGGAATA TAAATCTAAG 
GGAACTTTGA CTCTCCTGGC TCTAGGGGTG GAGAGAAAGG TACCATCCTT TCTTTCCTGG 
ATCTCCAACT CCAGCAATGT CACACTGGGG CATCTCATGT CTCCTCCCCA ATTTCTAGAG 
GATCCTCAGA GCCAAACTTT TTTCCTTGCT GAGGCCAAAT CAGAAATCCC CAGGCACCAG 
AGGGTTCTTG ATCCCAAGTT TGCTGAGGCA GGAATTGTGC TTTTAAAAAG CCCATCTGGC 
CTCTTGAGTC TGGTGCCCCT TTGGGAGTGC TGGAGCCTCA GCAAAGTCAG AGAATCAGCC 
TATTTTCCAG AACCAGAAGC CCTGGAAAGG AAATATCGCT CTGCCTTAGT TGAGAATCCC 
TCCTCAGCTA TGGAAGCCAT GGACCTGCCC TCACAGGCCC TTAAGACTCA AACCCCTTTC 
CTGGACCTAT TACATGAAGA CAAAGGGAAG GGCCAATTTG GAATTAGGTC TGTTCCTTTG 
ATTCAGACAA AGGCAGGAAT GGCCCATTCA GTGAAGGATG AAGTAACACA GATCCTGGAG 
GAGGAAAATA GAACCTCAAA GGGCCTGGGA GGCAGAGACC CTAAACTCTC TCCCTTGAGG 
CTGGGCTTTC ATGGTGATAT TCTGCCCTCG AGTAAGCCTT CCTGGAGGCC TAATACCTTC 
CAGGGGATAT CTGATGTCAA GAGTCTGCTA GGTCTGGAGA CTCAAACCAG TAAATGGGCC 
AGAGGGACAC TTTCTCTCTT GGCTACCTCA AGAAGGGGGA CAGAGTCAGT GAGAGGTACT 
TGGACAACAA CCCTCCATGG TGAGCCCAAG TTGAATGAAG AGCTCCCACA GGGCAGATTC 
AAAATTCAAG CTGGCCCCAG ATTGCCCTCG CTTTCCCTGC CTGAGCCTGT GATGGAGCCC 
CAGGTTACTG ACATCCCTTT TCTGTGGGAA CCTCACACCA AGAAGGGCAG AGGAGAGAGA 
AAGCCACATT CCCTAAGCCC TTCGAAGGCT GGACATGGCA ACATGCCTTT GACAGACTAC 
AACTCTGGGG AGCCATTTAG TGAGAACATA CTCCACCACC ATATGGCTTG GGTGGGGAGG 
CAGGGGGTCC AAGGAAACCA TCTGTCTCTG CAGAACCAAG CTTCTTCACA AATAATCCCC 
AAAGCGGTTC TGATTAGCCC AGTGTTGTAT GGAGCCAGAT GGATTCGGTT TTGGTACAGT 
CCCCTGCAGC CTGGCATGGG TGATGGTAGA GGGAGTTCAA GGCCCAGAAG ATGGTGCTCA 
TGTGAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ASTL cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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