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Genomic blot

A type of Southern blot specifically used to analyze a mixture of DNA fragments derived from total genomic DNA. Because genomic DNA is very complicated, when it has been digested with Restriction enzymes, it produces a complex set of fragments ranging from tens of bp to tens of thousands of bp. However, any specific gene will be reproducibly found on only one or a few specific fragments. A million identical Cells will produce a million identical Restriction fragments for any given gene, so probing a genomic Southern with a gene-specific probe will produce a pattern of perhaps one or just a few bands.


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