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Small-molecule control of super-Mendelian inheritance in gene drives

biorxiv. 2019; 
Víctor López Del Amo,  Brittany S. Leger,  Kurt J. Cox,  Shubhroz Gill,  Alena L. Bishop,  Garrett D. Scanlon,  James A. Walker,  Valentino M. Gantz,  Amit Choudhary
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Gene Synthesis The nanos-DD-SpCas9 transgenic constructs were made using the pnos-Cas9 (Addgene 62208) plasmid. Standard molecular biology techniques were used to build all the constructs analyzed in this work, and sequence information is uploaded on NCBI (accession number: 2183265). Portions of the engineered Cas9 constructs were synthetized using GenScript Inc. DNA Synthesis services. Get A Quote

Abstract

By surpassing the 50% inheritance limit of Mendel’s law of independent assortment, CRISPR-based gene drives have the potential to fight vector-borne diseases or suppress crop pests. However, contemporary gene drives could spread unchecked, posing safety concerns that limit their use in both laboratory and field settings. Current technologies also lack chemical control strategies, which could be applied in the field for dose, spatial and temporal control of gene drives. We describe in Drosophila the first gene-drive system controlled by an engineered Cas9 and a synthetic, orally-available small molecule.

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