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For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.tested for use in western blots [version 1; referees: awaiting peer
F1000Research. 2018;Products/Services Used Details Operation Peptide Synthesis Malate dehydrogenases (Mdhs) catalyse the interconversion of malate and oxaloacetate using NAD+ or NADH1 . In the yeast Saccharomyces cerevisiae there are three known isozymes: Mdh1 is located in mitochondria, Mdh2 is mostly cytosolic, and Mdh3 is localized to peroxisomes. The residues of the three isozymes are between 43–50% identical2 . It is thus very important for purposes of isolation and identification to utilize specific antibodies that will recognize only one specific isozyme. Using three Mdh2 peptides, which were specifically designed to unique regions in the Mdh2 protein, GenScript USA produced three different antibodies that should have high specificity for S. cerevisiae Mdh2 relative to Mdh1 and Mdh3. We then tested all three antibodies by western blotting and found one with specific binding. Due to its specificity, this antibody has the potential to also work in other experimental assays such as immunoprecipitation, immunohistochemistry and ELISA (EnzymeLinked Immunosorbent Assay). Get A Quote Abstract
Malate dehydrogenases (Mdhs) reversibly convert malate to oxaloacetate and serve as important enzymes in several metabolic pathways. In the yeast Saccharomyces cerevisiae there are three Mdh isozymes, localized to different compartments in the cell. In order to identify specifically the Mdh2 isozyme, GenScript USA produced three different antibodies that we further tested by western blot. All three antibodies recognized the S. cerevisiae Mdh2 with different background and specificity properties. One of the antibodies had a relatively low background and high specificity and thus can be used for specific identification of Mdh2 in ... More
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