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Gene Synthesis> | ... GGCAAACCGGAATTTGAATTCAGGTTTCCTGCAACGCTCCGA-3′ for NF-κB. Scrambled oligonucleotides were generated using https://www.genscript.com/ssl-bin/app/scramble and tested for absence of respective binding sites. Following consensus oligonucleotides were ... | Get A Quote |
Alternative promoter usage has been proposed as a mechanism regulating transcriptional and translational diversity in highly elaborated systems like the immune system in humans. Here, we report that transcription of human glycoprotein A repetitions predominant (GARP) in regulatory CD4 T cells (Tregs) is tightly regulated by two alternative promoters. An intragenic promoter contains several CpGs and acts as a weak promoter that is demethylated and initiates transcription Treg-specifically. The strong up-stream promoter containing a CpG-island is, in contrast, fully demethylated throughout tissues. Transcriptional activity of the strong promoter was surprisingly down-regulated upon demethylation of the weak promo... More