For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

Alternative promoter methylation regulates together with Foxp3 transcription of GARP.

Biochim Biophys Acta.. 2015-11; 
Haupt S , Söntgerath VS , Leipe J , Schulze-Koops H , Skapenko A .
Products/Services Used Details Operation
Gene Synthesis ... GGCAAACCGGAATTTGAATTCAGGTTTCCTGCAACGCTCCGA-3′ for NF-κB. Scrambled oligonucleotides were generated using https://www.genscript.com/ssl-bin/app/scramble and tested for absence of respective binding sites. Following consensus oligonucleotides were ... Get A Quote

Abstract

Alternative promoter usage has been proposed as a mechanism regulating transcriptional and translational diversity in highly elaborated systems like the immune system in humans. Here, we report that transcription of human glycoprotein A repetitions predominant (GARP) in regulatory CD4 T cells (Tregs) is tightly regulated by two alternative promoters. An intragenic promoter contains several CpGs and acts as a weak promoter that is demethylated and initiates transcription Treg-specifically. The strong up-stream promoter containing a CpG-island is, in contrast, fully demethylated throughout tissues. Transcriptional activity of the strong promoter was surprisingly down-regulated upon demethylation of the weak promo... More

Keywords