Tc00.1047053506241.19 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053506241.19
Entrez Gene ID	3550000
Full Name	dispersed gene family protein 1 (DGF-1)
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_812802.1	XP_817895.1	dispersed gene family protein 1 (DGF-1)

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053506241.19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053506241.19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb12277	XM_812802.1 Latest version!	Trypanosoma cruzi strain CL Brener dispersed gene family protein 1 (DGF-1) partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053506241.19 ( XM_812802.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_817895.1 Trypanosoma cruzi strain CL Brener dispersed gene family protein 1 (DGF-1) partial mRNA.

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CloneID	OTb12277
Clone ID Related Accession (Same CDS sequence)	XM_812802.1
Accession Version	XM_812802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	dispersed gene family protein 1 (DGF-1)
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849385). COMPLETENESS: incomplete on both ends.

ATGGTCGACG GGGTGAGAGC GACACAACAC GACAGTCCTG TTGGTGCCTG TTTGTGTCGG 
TGGATGCGGT CTGCGGAGGG TGCGTGTCCG CGCAGCCGGC ACCGTGTGCG GCGCAGGGCC 
ATTTTGGCTG TGCGTGCGGT GCTGTTTGTG GCGTTGGTGC TGGCTGCGGC GGCGTGGATG 
CCCGCGGTGC ATGCGGTGGT GCTGCGGCTG CGGGGCGGTG CGGTGGACAG GGCCATCATG 
GTTGGCCGCG CCATGGACAC AGTGCTGATG GACGGCGTGC ACATCACGAA CGGCGTGGCT 
GTGGTGTTTG ACGTGCCGGC GATGCTTCCC GGCGCGCTGC GCATTGAATT GAGGAACTGC 
GTGTGCGACG GCGGTGCGCA GATCTACGTG CGGGGCTACA GCGGCGAGCC GGCGAGTGAC 
CGGAGCCTGG AGGTGAGCGT GAGCGGGATG TCCGGCGGCT ACTGCTCGCT TGTGTTTGTG 
CACAACCTGC CGGCACACAC GAACGTGACG GTCCGTGACT CGACGATTGT GACGGCGGGG 
CCGATGCGCT ACTCCCAGCT GAAAATGTTG ACGGACGCGG TGGCGTCGCC GCTTGTGCTG 
TACGCGACGT CGCTGTTGCA GACGCAGCTG CGTGTGAGCA ACACTGTGCT GAGGTCGTTG 
CACGCGGGCG GGTCTGCGGT GTACGTTGGC GGCGGCGTGG ACCTGCTGTC GAGCGCGGTG 
GTGCTTGACG GCGTGTCGCT GGAGGCGTCG GGCGGTCCGA CCGCGTCCGC GATGCATGTG 
GCGTCCTCGT CGCGTCTCAG TCTGCGGAGC CACTCGGTGT TCTCCGTGAC GAACGTCTCG 
GTTGTGAGCA GCGGCGGTGG CATTGTGCTT GGCGAGCGCC TCGAGGTGGT TGATTCGGTG 
CTGCGCTTCG TGGGCGTCGA GGGGTCAGTT GCGTCGTCGC TGGTGCGCTG CGACGGTGGG 
ACGATCGGTG GCGGCGGGTG GCTGGAGTTG CGCGACGTGT GGGCGGTGGG CGAGGCGTCG 
TCGGTGGCGT CGCTGTCGGG GGTGAAGCTG AGCGGCGGCA CCGTGTCGAT TGCGCGCTGC 
GCTGCCACCG GCGCGACGCT TGTCTCCGGG CCGACGATCA CGAGCGGCGT CGTGTCGGTG 
CAGTGCAACC GTGCCGGCGG CCGGGTGCTG GAGAGCAGCG GCGACTACCG CTTGGCCGGC 
CTGCCCTCCG TGAGCGTGGT GCCGTGCGAC GGCTGTGCGG CTGCGCTGGC GTGCTTTGAT 
GCGCTGACGG CGTCGTTCTC CGACTGTGCG TGCAGCTGCC GTGCCGGCGG CGTGGGCGAG 
GCGTGCCTGC CGTTCGACGT GCCGGTTGCG AGGGCCGGTG GTGGTGCGCA GGACTGCGTG 
AGTGGCGTGA CGCTGACGGA GTCCGTGACG GTTGGCGGTG GGCGTGCGAC GGCGTGCTTC 
GACTCGGTGG TGTTCAGCGG GCCGATCACT GTGGCGGTGG ACCTGCGCTT GATGGACGCG 
TTCGCTGACG TGCTGAACGT GACGCTGCGC CGCTGCGTGC TTGCGGGCGG GTCGCAGCTG 
CGGATTGGCG GCCTCAGCGA GAGCACGGCG CGCCTGATGC CCCACGCCCT CGTCAACATG 
ACGAATGTGA CGTCGGTGGA GGGCACGATT GTGCTGCATG GCGCGGTGCC GCCGAACTCG 
AGTGTGCTGC TGGCGAACTC AACGCTGCGC GCGACGGTTG GCGGGTCGCA GTACGTGCCG 
ACGACCCCTG GCTTCGCGGG ATTTCGGTGT GGCCCCGCGC TTGTCCTGGA CGGTGTCCGG 
CTGCTGTCGA CGCGGTTTGT CATGACGCGG TCGACGCTGG TGTGTGGCGG TGGCTCGTGC 
GCTGCGATCC TCGTGGAGCG CGGCCTCGGC GTGCACCTGT CCAGCGTCTT CTACATGGAC 
AACTGCGTTG TCAATGCGCA GATGCACGTG ATGTACGCTT TTGGGTCGGA CCTGCTTGTC 
AGCGGCGGCT CCGTGTTCTC CATACAGAAC AGCTCGTGGA GTGTGTCGAG CATTAACATC 
TACAAAGGCG CGTGTGAGTT TGAGGGCGTT GTGGTGGATG GCGGCAGCGT GCTGCAGATT 
GTGTCCAGCA CTTTCCGTCT TGGCTTTGCC ATGCTCATTG CAAATACGCT GACCGTGACT 
GGTGGCAGTT GGCTAGTGCA CCGCGACAAC GAGTTCCGCA CCGCCTACGT TGTGTACGTG 
GTCAAGGAGA ACGGCGTGGC GTTCCGCGAT CGGAGTGTGT GGTCGATAAT TGACAACGAC 
CTCACGTACG GCTCGTATTT GTCGACCATC GCAAATATGA CGAGCAACTG GTCACCACCA 
TCCGACACGC GCCCGATCAT CTACGGCATG TGCAACGAGG CAAGGGGCTC TCCTGTGACG 
GATTATCAAG ACGACCTCAA TATTGGGACG CCCGTGAGGG TGTTGGACTG TGGTGCGTGC 
ACCATGGAGG CCGTGTGCTT CGCTGCGAGG ACGAGCAGCA TTAGCGGCTG TGGGTGTGTG 
TGCGCTGCTG GCGGCTACGG TGACACGTGT CTGCCGGCTG CTGTTCCGGA CGGCCTTGGG 
CCGCTCCCGC TCCCCGACGC GAAGGACACG GAGGTCCGCT GCGTGCACGG TGGCAGCATC 
AGCTCCGTGT ACGATCCTGA TCCCGGCGTG CGTTGTCTGT GCTTCGTCAA CGTGACTTTT 
ACCGCCGCGA TTGTGCTGGA ACTGTCATAT TTTGGCGCTC CAAAACAGAC ACTCAACATC 
ACGCTGCTGC AGTGCGTTCT CGTGGGCCTG TCGATCAAGG GGAGTGGTGT GCGAGTGCAC 
GTGAACGTGA CTTCTTCGAT GCTGGATTCT GGTGATTTGG AGTTCAGGGG TGATTTTGGC 
TTGAGCTCCC AGATACTGGT GGTTGGCAGT ACGCTTGTGT TGACGTCTGG CCACGCCATT 
CATTTCCAGA GGTTTTCTCT TGGTGTGAAC TCGTCGCTGC TGCTACTCGA CAACAACATT 
GAGGGGAGTA ATTACGCAGT CCGTTTATCC TTTGTTTTTG TTGATGGAGG CAGGATCATT 
ATGAAGGGAA ACAGGCTGAG GGGAGCGGAG GAGGACGTTA CTTTGGAATC CGCTGTTTTG 
TTTGCACCTG CTGTTTTGAA GAACGGGGGC TACTTTGACG TGGAGAACAA CACGATGATC 
GCAGTAAATG GCATTTATTT TTATGGGGAT ACCACCGTGA GCTCTGCGGG GCTGCTGAGA 
GTGGCGGACT GCGCCTTTGT CGGCAGGACA GAAATTTTTG ATTCCGCGCT GGTGCATTTG 
TCTGGCTCTG TGACACTCGA GGGTGGAGCG CAGTGGCGTG TGGAGGGCAA CAGCGTGAGC 
GCAGCCTCAG TGATAGGTAT GCCTAATTCC TTGTGCAAAA TGAAGCTGTC GGGCAGCGGC 
ACCACCGTGG TGCTTGCAAA CAACCGTCAG GTGGAATCGA GTAAAGCCTT TGCGAAACTT 
CTTCCACGGG GCACGATTGT GAAGCCGCCC GCGCGGTTTT TGGTTGGGTG CAACCTGCAG 
GGCGATGAGG AGGTGTCGTA CGGCGGTGTG TTTCCGGAGG GCGTGGTGGT GTTCAGGTGC 
GGCACGTGCG ACGACGACGC GGCGTGCCAC ATGCCCGGGA CGGAGTCGGT GGACCGCAGC 
TCGTGCTCGT GCAGCTGCAA GGACGGTTGG CATGGCGCGT CGTGTCTTCC CTTCGAGGTG 
CCCGACACGG TTGTGCCGCC CGTGGCGGAG AGAGCCGTTG ACGGCGACAC GAGCTGCGTG 
GTGAACCAGA CGCTGAAGTT CCTCACGCTG AACATGTGGA AGACGCACCA CTGCTACGCG 
GGTGTGACAT TCAGCGGCGT GGGTGCTTCG CTGACATTTT CATTCGACAG TATGCCGCTG 
CATCTCCCCA TCAACATCAC GCTGACTGGG TGCACATTCC GTGACGGTGC CGCGCTGCAG 
TTTGTTGGGG GTGCTGAGTC GGCGGAGTCA TCCGGCGTCC TGATCCGCGT GAGCCAGACG 
GTGATGCGGT CCTCTGTTAT TTTGTTTGCA CTTGCCCTCC CGAAGCACTG CGACATCACC 
GTCACGGAGG TTGACGCGGT GCAGTCTTCT GAGGTTCAGC TGCTTGACCT TATGAGCAAC 
ACGCTGAGCG TTGTGCTGCT GCAGAACTTT TTGTTGGCTG CGTCTTCGAT GTTGGTCAGC 
AACGTCAAGG CGCATGCATT GAGGTATGGG GGGTCTGGTT TGTACTCGAC CGGGACGCTG 
ACGCTGGTGG GTGGCAGCTC GCTGTACACG CGGTACTGCA GCTTCGATGG ATACGCACAC 
ATGCTGTACA TTAATATCCT CACTGTGAGT GATCACTCTG TTTTTGCGCT TCTCAACAAT 
AGAATTTCTT TTGGGGCAAG TCTTCTTTAT CTGCGCCATG GATTCAGCGT GTCGGATCAC 
AGTGTGTTGC GCGTGGTGGG GAACAGCGTT TTTTCATTCT ATGCTATTTA CGCTAACAAA 
TTTTGGACCG TTGAGCGATC AAGCTGGTTG GATTGGCGTG ACAACGACGT GGGAGTGGGC 
GCGATTTTCA AAGACTGTGA TCCCACCTTT GTGAGCATTG ACAGCAGCAG TGTGGTGACG 
CTGACGGGTT GCAATATGGG ATCGACGGGG TTGTCGGGGC CCCTGCTGTC GCGGGTTGGG 
GCCGGTTACC GGTTCGTTGC TGGGTGCCTG ACGGTCGCGG GACGGGAGGT GACGACGGCG 
GCTGAACTGG AGCTGAACGG CATCACCAAC GTGACGAATG TTGCCGCGTG CGGGGAGTGC 
ACGAAGGAGG GCGACTGCTT TGCTCCGCTG ACGACGGCGA TCATTGACTG CAAGTGCCAG 
TGCGCTGCTG GAGGGCACGG CGATGTGTGC GTCCCGGCTC CTGTGCCTGC TGGCCCGCCA 
CCGCCGCCAA CAACACCACC ACCAGCAACA CCCACGCCAC CGCCGGTTGG TGAGTGCATC 
AGCGACATGG TGTACCCCGA GGTTGCGCAG GCAGTGGGCG GCGGGCTGTC GTGGCTGTGC 
TACCGCAACG TGACGTTCAG CGGGGGCGGC ATGAGCATGA CGGTGCTGGT CGGGGCGATG 
ACGGGCGACG TGGTGAATGT CATGTTTGAT GGATGCACTT GGAGGGACGG CGCCGTGCTG 
TTGCTCTTGG GCAACGTGCA CGCCGCTGTG GGGTCGCTGA ACATCGTCGT CACCGGCAAC 
ACATTTAGTG ACGCGCTGCT CAGCCCGGAG GGTGGGTTTC CACCGCACAC AAACATCACG 
ATCAGCGGGA ACCGCTTCAT TGTCACGAGG CTGATCCCTC GGTTTTGTTT GGGCGTTAGG 
AGGCCGTCGT GTGTTGCGAT GAATGGACTG GCGATCAGCA ATGACTCCGC GGTGGTGCTG 
AGCGGCAATG TGTTTCACGC AGTGACGGCA TCGTCAAGCG CCATTTACGT TGTCAGGTCT 
GCGCTGAGCG TGTCGTGGCA CTCCGTGTTT GCGGTGTTGG GCAACACGTT TCATATGGCT 
GGCGGTGACG GTGCGCCGAT ACATCTTGAA GGGTCTAGCC AATCCTCATC GCTGAAGGTT 
CTGAACAACT CTGCCGTGGT GATCCGAGGC AACGTTGTGT CGAGGCCGGT GCAGTGCTTT 
GTGTTATTTA TTCGGACTTT AGACGTGGAG TCTCTTTCAG CTATTGTGTT TCAGGGGAAC 
GAGATGCAGG GAAGCTCGGT TGTGTTTTAT TCAGGCGAAT CGTCCAACAT CTACTACAAC 
TCCTGGTTGC AGTTGAGTGG CAACCTCTGC CGTGAATCCC CATCGGAAGC CCTTGCTTTT 
TTTTACTCCA CGGTGAATCT CCGCGACTCC ACGGTGTCTG TTTTTGGCAA CCAATTCATT 
TCCAGCACGG GAATGACGAA AGCGCTGTGG ATACATGCAG TTTCCAGCGA CCTCAAAAAC 
GGAGAGATTG TGGCTGCGTG CAACAGTGTG AACGGCGAGG AGGAGGCGAA CTACGCCATT 
CCGTCTGTGT ACAACGCCAC ATTTCTGACG TGCAGCGACC CATGCACCCT CGCGGCGTCG 
TGCTTCCCCG CGTACACGAC GACGGCGTCG AGTGATGGCT GCGCCTGCAC GTGCGCGGAG 
GGCGGCCATG GCGATGCATG CCTGCCTGTT GCTGTTCCCG AGCCACCGAG CACCGACGGA 
GCCGACTTGT GCGTGCGGGA CGTGCGTGTG GAGGTGGGGG TGAGCGCCGG TCTCGGGACG 
TCGGTGGCGT GCTACGTTGG CGTGACCTTT GCGGCGGACG TCGTGGTGGA TGTGGAGTCG 
ATGTCGGGGA GCGTGCGCAA CGTGACGCTG GCGAACTGTA CGTTTGTGGG CGGAGCGAGC 
CTGTACGTTT TGGGGTGGCG TTCCGACTCG CCTGCTGGCG AGCGCGCCGA TGTGTTGATC 
AGCGGGCTTG AGTCGCGCTC CGGCGGCGGC GTGTTGGTGG CGAACCGATT TCCGCCGGGC 
TCGCGCGTCA CTGTGGTGGA CTCGGTGCTG ATTGCAGAGA AGCGTGTTGC GTACCGCGAC 
GCCTACGACC TTGGCGACGT CTCCGCGTGC CTCGTGGTGC ACAGCGTGAA TCTGACTGGG 
AGCGTGCTGA CCATTGCGCG CACGCACGTG GCGGCTGTGT TCGGCGACGC TGTTGGCGTG 
TTGTTGGTTG GCGGCGTGGC GCTGCAGTCA CGTGGTGCGC TGTACGTGGA CGGACTGTCG 
GTGCAGACGG CGCTGGGGCT GTGCGTGTCG GTGGAGGGCG GTGTTTCGGC CAGCGGCGGC 
TCCGTTGTGG CGTTTGTTGA CAGCGACTTC CTGCTGTGCA AGCACGCGGT GTGTGTGCGT 
GGGGCTGTGA GCGTGTCGGG CTCCGCCGTG GCGCTTGTGC GGAGCGACTT TTCGTTGACG 
GAGGACTACG CGGTGGCGTT TTATTCGACG GTGAGCCTGG ATGGCGGGTC GATGCTGCTG 
GCGAAGGGCA ACGTGCACGA CGGCGTCTCG AGGGAGATGC TCCACGCCGC TGGCGCCGTG 
ACCGCTGCTG GGAGCACGCT GAGCTTTGTG CGCAACCGAG CGCTGCTGCC GCGGATGCTG 
TCGCTGAGCC TGTCGCTTGC GGCTGGGGCG CATGTGAGGG TGGCGTGCAA CTACGCGGGT 
GGACGTGTCC TGTCGACGGC GGAGGAGTAC GCTGCTGCTG GATTTGGCGA CGCTGGGCGC 
ATCGACGTTG CTGGGTGCGA CGCCTGCGAC AGGGACACGC ACTGCTACGC GCCCGGGACG 
GCGTCGGCCA GCATGAAGGA CGGTGTGTGC GAGTGCGTGT GCGGCAGCGG CGGGTACGGC 
GAGGCATGCG TGCCTGTGGG TGCTCCCGTG CTGCCGACCG CTGTGGGCAC TGCGTCGAGT 
TTGTTTGTCC GTGAGGGCGT GACGGTGCGG TCGGTGTTCG TTGTGCCTGC CGGCGCGAGC 
GAGGTGACGC TGCGCCACGT TGTGCTGGAC GGCGTGAGTC CGGTGCTCTA CGTGCCGTGG 
ATGGCGCGGG ACGGCGTGCG GATTGTTGTG CAGAACGTGT CGCTGCTGAA CGGCGCCGTG 
CTGTACGTGA TGGGCGGTGG AGGCCTGCGC GGTGCGGTGG CGGCGGGGAG CGACGAGGGC 
GGGCCGGTGG AGCTGTCGGT GTGCGATGTG GAGGCGCTGA ACGGTGCGCT TGTGCTGACC 
GGCACGTTCC CCGCGGGGTC CGTGCTGACG GTGACGGACT CCGTGCTGGT TGCCGCGAGG 
CCGACGCCGC TTGTGTATCT TCCCGGCTCG CGGTCCTCGC CGTACGCACC GGTGCTGGTG 
CTGTCGGGTC TGCGGCTCGT GCGGTCCGTG CTGGTTGTGT CCGGCGTTGC CCTCGTGACG 
GTGGTGACTG GTGGGCGGAC GGTGGTGGTG GACGGCGCCG TGCTGGAGCT TGTCGGTGGC 
GGTGTCGCGC TGGACGCGGC TGTGTTCGGT GGCGACTATG CGTTGTACGC GAGTGCGCGC 
GTGGTTGCGT CTGAGGGCGC TGTGCTGCGC GTGTCGGGGA GCCAGGTGTA CGCCGCGCAT 
GGATTGTTGT TCGAGAGCGG TGTGGAGGCC AACGCGTCCG CCGTCGTGGT GAACGACAAC 
GCCGGTGCGC TGACCGATGG CGCGCTGCTG GTGCTGCGGG GGTCGGCGTC GTTCGTGTCC 
GGGTCGTGGC TGTCGGTGCG CGGCAACAGC ATCAGCGGCA GGCTCCTGTC GCTGCCGTCG 
TACCCGCGCC GTGCGGAGCT CGTGCAGAGC ACGCTGACGC TTCACAAGAA CGCTGGCAGC 
GGACCCGTCG TGATGGACGG CACCGTTGCG CTCGGGGGTG CCGGCCGAAA GTTTGTCGTG 
GGGTGCCTGA CGCTGAACGG GCATGCGCTG CAGCCGATGG ACTACAGATC CGCCGGCATC 
ATCGGGAAAT TCCGCCCCGT GGCGTGTGGC GTTTGCGATG CCGACGTGCG CTGCTTTGCA 
GCTGCGACGA GGGCGATGTC GGGGTCGTGC GGCTGCCGCT GTGCTGAGGG CGGGTACGGG 
CGCGACTGCC TGCCGGTGTA CCTGCCGCAC GTGGACGGGT GCAACCGCAC GCCCGCCCTG 
CCGCTGCTGA GCCACACGGA GACGCTGACG GAGACGCGCA GCCTGACGCC GGGCATGAGT 
ACGACGCACT ACAGTCCGAC GCAGTACAGT TCGACGGAGA CCCTGCAGGT GACGGAGACG 
GTGGCGCTGT CGCCCACGCG GACGCCGACG GCATCGGTGA GCAGCACGCT GTGGTGGAGC 
GACGTGGCGT GCCCGACGCT TGCCGTGACG ACGACGGCGG CTGGTGGGAG CCTGACGCAG 
AACGACATTC GCGGCGGTGG GAGCGCCGTC CCGACGCGGC TGATGGTGGC GCTGCCGCCG 
CCGTTTCGGT GGGCACGCGA CCCGCAGCTT GGCACGCACC TGAGCTTCGT GCCGGTGTCG 
ACGGCGCAGC CGCGCGGGAT CGGTGGTCCG TGGGGAGCGA TGCTGAGTAA CGCGAAGTGG 
GTGCGCAACG CGACGAATCC GTCCACCGTT CTGGAGCTGG CTGTGCCCGT GCACCGCGGT 
TACTTCATTG CTGCCGACGA GACGATATTC ATTCGCTGCG ACGCTGTGGC GGTGTTCGGC 
GGCTGCAAGG GTGTGCTGCT TGGGTCCTTC ACGATCAGGT CAAACACGCT GCCCGCCGCT 
GCCAGCGTCG TCTCGGCGAT CACCGGCGTT GTTGCGGGTG CGGCGGCTGT TGCCGTGGTG 
GTGACCGGCG GACTGGGCTC CATTCTGGAG ATGCAGGCGC TCGGCGTGTT CGCGAGGATG 
CCATGCGCCT CGGCGCAGGA GCGTGCGAGC ACCGTTGCGC TGCCTTACTT CCTGTCGGTG 
TTCGCTGCCC GTGACCCGCT GTGGATGGTG GTGGGCAACG CGCTGCTCGC CGCTGTGTTC 
GGGTGCGTGC ACTGCGGTGT GACGGCGGCC TTTCAGCGGT GGCGCGGCGT GGACGCGGCG 
AGTGCGTGGG CTGCGATGCG CTTCCCGAGC CTGACGTACG TCGTGGCGCA CGCGATGCAC 
CTCGGCATCT TCTTTGGCTC CGTGCTTGCA CTGGCGATGC CCGACGCCCG CGTGCAGCAC 
CGCGTAATTG GTGTCGTTGG CGTGCTGTAC GGTGCGGCGT TCCCTGCTGG CGTGTGCTAC 
TTGATTGCCC GCCACGTGGG CGCGAGCTTC ACGAGTTACT GGCGGTTTTC GAGGAAGCCG 
CTGCACGAGC GCCTGCTGTA CCCCGTTGGG TATTGGCACC CCGCGGCGCA GCAGCGGATG 
TACGGCGGCA TGCTGACGAA CATGAGGGGC AGCTGCGTGT ACTGGTGCGT GTTCCAGCTG 
TCGGTGTTGT GCGTGGTGTG CCTGATTGCG GCTGTGCACC CGCCCGTGGG AGGCTGCCAC 
GTCCAGTATT TCTGCACGGC GGCTGTGCTG CTTGCGGGTG CGGGCGTGAT TGCCTTCACG 
AACATGATGC GCTCGGCCTT CCTGACGGTG ATGCACACTG CGGGCTTTGT ACTCCTTGCG 
GCGCTGTGCG TGGTCAGCGC GGCCAACCAC CTTGCGCCGA GCGACGGCGG TGCGAGGGCG 
TACGCGGCTA TCGTGCTGCT GCTGACGACT GTGCCGCTTT TGGCGACTGT GTACAGCGTC 
GTTGTGTGGT ATGCGGAGGA CCGCCACTGG CAGGAGCTGC GCGAGCCGCG GCGTGGAGGG 
CTGGAGGCGC TGCTGCGCGA TGACGAGGAT GGCGACGAGG AGACGCAGAA GCCCCACGAA 
ATGACGTCGT CGTACGCCTC AGGCACCACC GTTGCGTCGT TGTACCGACC ACCCGCACCG 
CCGTTGCAAC CCATGGCGGT GACACCCACT CCGACACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_817895.1
CDS	1..10362
Translation	MVDGVRATQHDSPVGACLCRWMRSAEGACPRSRHRVRRRAILAVRAVLFVALVLAAAAWMPAVHAVVLRLRGGAVDRAIMVGRAMDTVLMDGVHITNGVAVVFDVPAMLPGALRIELRNCVCDGGAQIYVRGYSGEPASDRSLEVSVSGMSGGYCSLVFVHNLPAHTNVTVRDSTIVTAGPMRYSQLKMLTDAVASPLVLYATSLLQTQLRVSNTVLRSLHAGGSAVYVGGGVDLLSSAVVLDGVSLEASGGPTASAMHVASSSRLSLRSHSVFSVTNVSVVSSGGGIVLGERLEVVDSVLRFVGVEGSVASSLVRCDGGTIGGGGWLELRDVWAVGEASSVASLSGVKLSGGTVSIARCAATGATLVSGPTITSGVVSVQCNRAGGRVLESSGDYRLAGLPSVSVVPCDGCAAALACFDALTASFSDCACSCRAGGVGEACLPFDVPVARAGGGAQDCVSGVTLTESVTVGGGRATACFDSVVFSGPITVAVDLRLMDAFADVLNVTLRRCVLAGGSQLRIGGLSESTARLMPHALVNMTNVTSVEGTIVLHGAVPPNSSVLLANSTLRATVGGSQYVPTTPGFAGFRCGPALVLDGVRLLSTRFVMTRSTLVCGGGSCAAILVERGLGVHLSSVFYMDNCVVNAQMHVMYAFGSDLLVSGGSVFSIQNSSWSVSSINIYKGACEFEGVVVDGGSVLQIVSSTFRLGFAMLIANTLTVTGGSWLVHRDNEFRTAYVVYVVKENGVAFRDRSVWSIIDNDLTYGSYLSTIANMTSNWSPPSDTRPIIYGMCNEARGSPVTDYQDDLNIGTPVRVLDCGACTMEAVCFAARTSSISGCGCVCAAGGYGDTCLPAAVPDGLGPLPLPDAKDTEVRCVHGGSISSVYDPDPGVRCLCFVNVTFTAAIVLELSYFGAPKQTLNITLLQCVLVGLSIKGSGVRVHVNVTSSMLDSGDLEFRGDFGLSSQILVVGSTLVLTSGHAIHFQRFSLGVNSSLLLLDNNIEGSNYAVRLSFVFVDGGRIIMKGNRLRGAEEDVTLESAVLFAPAVLKNGGYFDVENNTMIAVNGIYFYGDTTVSSAGLLRVADCAFVGRTEIFDSALVHLSGSVTLEGGAQWRVEGNSVSAASVIGMPNSLCKMKLSGSGTTVVLANNRQVESSKAFAKLLPRGTIVKPPARFLVGCNLQGDEEVSYGGVFPEGVVVFRCGTCDDDAACHMPGTESVDRSSCSCSCKDGWHGASCLPFEVPDTVVPPVAERAVDGDTSCVVNQTLKFLTLNMWKTHHCYAGVTFSGVGASLTFSFDSMPLHLPINITLTGCTFRDGAALQFVGGAESAESSGVLIRVSQTVMRSSVILFALALPKHCDITVTEVDAVQSSEVQLLDLMSNTLSVVLLQNFLLAASSMLVSNVKAHALRYGGSGLYSTGTLTLVGGSSLYTRYCSFDGYAHMLYINILTVSDHSVFALLNNRISFGASLLYLRHGFSVSDHSVLRVVGNSVFSFYAIYANKFWTVERSSWLDWRDNDVGVGAIFKDCDPTFVSIDSSSVVTLTGCNMGSTGLSGPLLSRVGAGYRFVAGCLTVAGREVTTAAELELNGITNVTNVAACGECTKEGDCFAPLTTAIIDCKCQCAAGGHGDVCVPAPVPAGPPPPPTTPPPATPTPPPVGECISDMVYPEVAQAVGGGLSWLCYRNVTFSGGGMSMTVLVGAMTGDVVNVMFDGCTWRDGAVLLLLGNVHAAVGSLNIVVTGNTFSDALLSPEGGFPPHTNITISGNRFIVTRLIPRFCLGVRRPSCVAMNGLAISNDSAVVLSGNVFHAVTASSSAIYVVRSALSVSWHSVFAVLGNTFHMAGGDGAPIHLEGSSQSSSLKVLNNSAVVIRGNVVSRPVQCFVLFIRTLDVESLSAIVFQGNEMQGSSVVFYSGESSNIYYNSWLQLSGNLCRESPSEALAFFYSTVNLRDSTVSVFGNQFISSTGMTKALWIHAVSSDLKNGEIVAACNSVNGEEEANYAIPSVYNATFLTCSDPCTLAASCFPAYTTTASSDGCACTCAEGGHGDACLPVAVPEPPSTDGADLCVRDVRVEVGVSAGLGTSVACYVGVTFAADVVVDVESMSGSVRNVTLANCTFVGGASLYVLGWRSDSPAGERADVLISGLESRSGGGVLVANRFPPGSRVTVVDSVLIAEKRVAYRDAYDLGDVSACLVVHSVNLTGSVLTIARTHVAAVFGDAVGVLLVGGVALQSRGALYVDGLSVQTALGLCVSVEGGVSASGGSVVAFVDSDFLLCKHAVCVRGAVSVSGSAVALVRSDFSLTEDYAVAFYSTVSLDGGSMLLAKGNVHDGVSREMLHAAGAVTAAGSTLSFVRNRALLPRMLSLSLSLAAGAHVRVACNYAGGRVLSTAEEYAAAGFGDAGRIDVAGCDACDRDTHCYAPGTASASMKDGVCECVCGSGGYGEACVPVGAPVLPTAVGTASSLFVREGVTVRSVFVVPAGASEVTLRHVVLDGVSPVLYVPWMARDGVRIVVQNVSLLNGAVLYVMGGGGLRGAVAAGSDEGGPVELSVCDVEALNGALVLTGTFPAGSVLTVTDSVLVAARPTPLVYLPGSRSSPYAPVLVLSGLRLVRSVLVVSGVALVTVVTGGRTVVVDGAVLELVGGGVALDAAVFGGDYALYASARVVASEGAVLRVSGSQVYAAHGLLFESGVEANASAVVVNDNAGALTDGALLVLRGSASFVSGSWLSVRGNSISGRLLSLPSYPRRAELVQSTLTLHKNAGSGPVVMDGTVALGGAGRKFVVGCLTLNGHALQPMDYRSAGIIGKFRPVACGVCDADVRCFAAATRAMSGSCGCRCAEGGYGRDCLPVYLPHVDGCNRTPALPLLSHTETLTETRSLTPGMSTTHYSPTQYSSTETLQVTETVALSPTRTPTASVSSTLWWSDVACPTLAVTTTAAGGSLTQNDIRGGGSAVPTRLMVALPPPFRWARDPQLGTHLSFVPVSTAQPRGIGGPWGAMLSNAKWVRNATNPSTVLELAVPVHRGYFIAADETIFIRCDAVAVFGGCKGVLLGSFTIRSNTLPAAASVVSAITGVVAGAAAVAVVVTGGLGSILEMQALGVFARMPCASAQERASTVALPYFLSVFAARDPLWMVVGNALLAAVFGCVHCGVTAAFQRWRGVDAASAWAAMRFPSLTYVVAHAMHLGIFFGSVLALAMPDARVQHRVIGVVGVLYGAAFPAGVCYLIARHVGASFTSYWRFSRKPLHERLLYPVGYWHPAAQQRMYGGMLTNMRGSCVYWCVFQLSVLCVVCLIAAVHPPVGGCHVQYFCTAAVLLAGAGVIAFTNMMRSAFLTVMHTAGFVLLAALCVVSAANHLAPSDGGARAYAAIVLLLTTVPLLATVYSVVVWYAEDRHWQELREPRRGGLEALLRDDEDGDEETQKPHEMTSSYASGTTVASLYRPPAPPLQPMAVTPTPTL

Target ORF information:

RefSeq Version	XM_812802.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener dispersed gene family protein 1 (DGF-1) partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_812802.1

ORF Insert Sequence:

ATGGTCGACG GGGTGAGAGC GACACAACAC GACAGTCCTG TTGGTGCCTG TTTGTGTCGG 
TGGATGCGGT CTGCGGAGGG TGCGTGTCCG CGCAGCCGGC ACCGTGTGCG GCGCAGGGCC 
ATTTTGGCTG TGCGTGCGGT GCTGTTTGTG GCGTTGGTGC TGGCTGCGGC GGCGTGGATG 
CCCGCGGTGC ATGCGGTGGT GCTGCGGCTG CGGGGCGGTG CGGTGGACAG GGCCATCATG 
GTTGGCCGCG CCATGGACAC AGTGCTGATG GACGGCGTGC ACATCACGAA CGGCGTGGCT 
GTGGTGTTTG ACGTGCCGGC GATGCTTCCC GGCGCGCTGC GCATTGAATT GAGGAACTGC 
GTGTGCGACG GCGGTGCGCA GATCTACGTG CGGGGCTACA GCGGCGAGCC GGCGAGTGAC 
CGGAGCCTGG AGGTGAGCGT GAGCGGGATG TCCGGCGGCT ACTGCTCGCT TGTGTTTGTG 
CACAACCTGC CGGCACACAC GAACGTGACG GTCCGTGACT CGACGATTGT GACGGCGGGG 
CCGATGCGCT ACTCCCAGCT GAAAATGTTG ACGGACGCGG TGGCGTCGCC GCTTGTGCTG 
TACGCGACGT CGCTGTTGCA GACGCAGCTG CGTGTGAGCA ACACTGTGCT GAGGTCGTTG 
CACGCGGGCG GGTCTGCGGT GTACGTTGGC GGCGGCGTGG ACCTGCTGTC GAGCGCGGTG 
GTGCTTGACG GCGTGTCGCT GGAGGCGTCG GGCGGTCCGA CCGCGTCCGC GATGCATGTG 
GCGTCCTCGT CGCGTCTCAG TCTGCGGAGC CACTCGGTGT TCTCCGTGAC GAACGTCTCG 
GTTGTGAGCA GCGGCGGTGG CATTGTGCTT GGCGAGCGCC TCGAGGTGGT TGATTCGGTG 
CTGCGCTTCG TGGGCGTCGA GGGGTCAGTT GCGTCGTCGC TGGTGCGCTG CGACGGTGGG 
ACGATCGGTG GCGGCGGGTG GCTGGAGTTG CGCGACGTGT GGGCGGTGGG CGAGGCGTCG 
TCGGTGGCGT CGCTGTCGGG GGTGAAGCTG AGCGGCGGCA CCGTGTCGAT TGCGCGCTGC 
GCTGCCACCG GCGCGACGCT TGTCTCCGGG CCGACGATCA CGAGCGGCGT CGTGTCGGTG 
CAGTGCAACC GTGCCGGCGG CCGGGTGCTG GAGAGCAGCG GCGACTACCG CTTGGCCGGC 
CTGCCCTCCG TGAGCGTGGT GCCGTGCGAC GGCTGTGCGG CTGCGCTGGC GTGCTTTGAT 
GCGCTGACGG CGTCGTTCTC CGACTGTGCG TGCAGCTGCC GTGCCGGCGG CGTGGGCGAG 
GCGTGCCTGC CGTTCGACGT GCCGGTTGCG AGGGCCGGTG GTGGTGCGCA GGACTGCGTG 
AGTGGCGTGA CGCTGACGGA GTCCGTGACG GTTGGCGGTG GGCGTGCGAC GGCGTGCTTC 
GACTCGGTGG TGTTCAGCGG GCCGATCACT GTGGCGGTGG ACCTGCGCTT GATGGACGCG 
TTCGCTGACG TGCTGAACGT GACGCTGCGC CGCTGCGTGC TTGCGGGCGG GTCGCAGCTG 
CGGATTGGCG GCCTCAGCGA GAGCACGGCG CGCCTGATGC CCCACGCCCT CGTCAACATG 
ACGAATGTGA CGTCGGTGGA GGGCACGATT GTGCTGCATG GCGCGGTGCC GCCGAACTCG 
AGTGTGCTGC TGGCGAACTC AACGCTGCGC GCGACGGTTG GCGGGTCGCA GTACGTGCCG 
ACGACCCCTG GCTTCGCGGG ATTTCGGTGT GGCCCCGCGC TTGTCCTGGA CGGTGTCCGG 
CTGCTGTCGA CGCGGTTTGT CATGACGCGG TCGACGCTGG TGTGTGGCGG TGGCTCGTGC 
GCTGCGATCC TCGTGGAGCG CGGCCTCGGC GTGCACCTGT CCAGCGTCTT CTACATGGAC 
AACTGCGTTG TCAATGCGCA GATGCACGTG ATGTACGCTT TTGGGTCGGA CCTGCTTGTC 
AGCGGCGGCT CCGTGTTCTC CATACAGAAC AGCTCGTGGA GTGTGTCGAG CATTAACATC 
TACAAAGGCG CGTGTGAGTT TGAGGGCGTT GTGGTGGATG GCGGCAGCGT GCTGCAGATT 
GTGTCCAGCA CTTTCCGTCT TGGCTTTGCC ATGCTCATTG CAAATACGCT GACCGTGACT 
GGTGGCAGTT GGCTAGTGCA CCGCGACAAC GAGTTCCGCA CCGCCTACGT TGTGTACGTG 
GTCAAGGAGA ACGGCGTGGC GTTCCGCGAT CGGAGTGTGT GGTCGATAAT TGACAACGAC 
CTCACGTACG GCTCGTATTT GTCGACCATC GCAAATATGA CGAGCAACTG GTCACCACCA 
TCCGACACGC GCCCGATCAT CTACGGCATG TGCAACGAGG CAAGGGGCTC TCCTGTGACG 
GATTATCAAG ACGACCTCAA TATTGGGACG CCCGTGAGGG TGTTGGACTG TGGTGCGTGC 
ACCATGGAGG CCGTGTGCTT CGCTGCGAGG ACGAGCAGCA TTAGCGGCTG TGGGTGTGTG 
TGCGCTGCTG GCGGCTACGG TGACACGTGT CTGCCGGCTG CTGTTCCGGA CGGCCTTGGG 
CCGCTCCCGC TCCCCGACGC GAAGGACACG GAGGTCCGCT GCGTGCACGG TGGCAGCATC 
AGCTCCGTGT ACGATCCTGA TCCCGGCGTG CGTTGTCTGT GCTTCGTCAA CGTGACTTTT 
ACCGCCGCGA TTGTGCTGGA ACTGTCATAT TTTGGCGCTC CAAAACAGAC ACTCAACATC 
ACGCTGCTGC AGTGCGTTCT CGTGGGCCTG TCGATCAAGG GGAGTGGTGT GCGAGTGCAC 
GTGAACGTGA CTTCTTCGAT GCTGGATTCT GGTGATTTGG AGTTCAGGGG TGATTTTGGC 
TTGAGCTCCC AGATACTGGT GGTTGGCAGT ACGCTTGTGT TGACGTCTGG CCACGCCATT 
CATTTCCAGA GGTTTTCTCT TGGTGTGAAC TCGTCGCTGC TGCTACTCGA CAACAACATT 
GAGGGGAGTA ATTACGCAGT CCGTTTATCC TTTGTTTTTG TTGATGGAGG CAGGATCATT 
ATGAAGGGAA ACAGGCTGAG GGGAGCGGAG GAGGACGTTA CTTTGGAATC CGCTGTTTTG 
TTTGCACCTG CTGTTTTGAA GAACGGGGGC TACTTTGACG TGGAGAACAA CACGATGATC 
GCAGTAAATG GCATTTATTT TTATGGGGAT ACCACCGTGA GCTCTGCGGG GCTGCTGAGA 
GTGGCGGACT GCGCCTTTGT CGGCAGGACA GAAATTTTTG ATTCCGCGCT GGTGCATTTG 
TCTGGCTCTG TGACACTCGA GGGTGGAGCG CAGTGGCGTG TGGAGGGCAA CAGCGTGAGC 
GCAGCCTCAG TGATAGGTAT GCCTAATTCC TTGTGCAAAA TGAAGCTGTC GGGCAGCGGC 
ACCACCGTGG TGCTTGCAAA CAACCGTCAG GTGGAATCGA GTAAAGCCTT TGCGAAACTT 
CTTCCACGGG GCACGATTGT GAAGCCGCCC GCGCGGTTTT TGGTTGGGTG CAACCTGCAG 
GGCGATGAGG AGGTGTCGTA CGGCGGTGTG TTTCCGGAGG GCGTGGTGGT GTTCAGGTGC 
GGCACGTGCG ACGACGACGC GGCGTGCCAC ATGCCCGGGA CGGAGTCGGT GGACCGCAGC 
TCGTGCTCGT GCAGCTGCAA GGACGGTTGG CATGGCGCGT CGTGTCTTCC CTTCGAGGTG 
CCCGACACGG TTGTGCCGCC CGTGGCGGAG AGAGCCGTTG ACGGCGACAC GAGCTGCGTG 
GTGAACCAGA CGCTGAAGTT CCTCACGCTG AACATGTGGA AGACGCACCA CTGCTACGCG 
GGTGTGACAT TCAGCGGCGT GGGTGCTTCG CTGACATTTT CATTCGACAG TATGCCGCTG 
CATCTCCCCA TCAACATCAC GCTGACTGGG TGCACATTCC GTGACGGTGC CGCGCTGCAG 
TTTGTTGGGG GTGCTGAGTC GGCGGAGTCA TCCGGCGTCC TGATCCGCGT GAGCCAGACG 
GTGATGCGGT CCTCTGTTAT TTTGTTTGCA CTTGCCCTCC CGAAGCACTG CGACATCACC 
GTCACGGAGG TTGACGCGGT GCAGTCTTCT GAGGTTCAGC TGCTTGACCT TATGAGCAAC 
ACGCTGAGCG TTGTGCTGCT GCAGAACTTT TTGTTGGCTG CGTCTTCGAT GTTGGTCAGC 
AACGTCAAGG CGCATGCATT GAGGTATGGG GGGTCTGGTT TGTACTCGAC CGGGACGCTG 
ACGCTGGTGG GTGGCAGCTC GCTGTACACG CGGTACTGCA GCTTCGATGG ATACGCACAC 
ATGCTGTACA TTAATATCCT CACTGTGAGT GATCACTCTG TTTTTGCGCT TCTCAACAAT 
AGAATTTCTT TTGGGGCAAG TCTTCTTTAT CTGCGCCATG GATTCAGCGT GTCGGATCAC 
AGTGTGTTGC GCGTGGTGGG GAACAGCGTT TTTTCATTCT ATGCTATTTA CGCTAACAAA 
TTTTGGACCG TTGAGCGATC AAGCTGGTTG GATTGGCGTG ACAACGACGT GGGAGTGGGC 
GCGATTTTCA AAGACTGTGA TCCCACCTTT GTGAGCATTG ACAGCAGCAG TGTGGTGACG 
CTGACGGGTT GCAATATGGG ATCGACGGGG TTGTCGGGGC CCCTGCTGTC GCGGGTTGGG 
GCCGGTTACC GGTTCGTTGC TGGGTGCCTG ACGGTCGCGG GACGGGAGGT GACGACGGCG 
GCTGAACTGG AGCTGAACGG CATCACCAAC GTGACGAATG TTGCCGCGTG CGGGGAGTGC 
ACGAAGGAGG GCGACTGCTT TGCTCCGCTG ACGACGGCGA TCATTGACTG CAAGTGCCAG 
TGCGCTGCTG GAGGGCACGG CGATGTGTGC GTCCCGGCTC CTGTGCCTGC TGGCCCGCCA 
CCGCCGCCAA CAACACCACC ACCAGCAACA CCCACGCCAC CGCCGGTTGG TGAGTGCATC 
AGCGACATGG TGTACCCCGA GGTTGCGCAG GCAGTGGGCG GCGGGCTGTC GTGGCTGTGC 
TACCGCAACG TGACGTTCAG CGGGGGCGGC ATGAGCATGA CGGTGCTGGT CGGGGCGATG 
ACGGGCGACG TGGTGAATGT CATGTTTGAT GGATGCACTT GGAGGGACGG CGCCGTGCTG 
TTGCTCTTGG GCAACGTGCA CGCCGCTGTG GGGTCGCTGA ACATCGTCGT CACCGGCAAC 
ACATTTAGTG ACGCGCTGCT CAGCCCGGAG GGTGGGTTTC CACCGCACAC AAACATCACG 
ATCAGCGGGA ACCGCTTCAT TGTCACGAGG CTGATCCCTC GGTTTTGTTT GGGCGTTAGG 
AGGCCGTCGT GTGTTGCGAT GAATGGACTG GCGATCAGCA ATGACTCCGC GGTGGTGCTG 
AGCGGCAATG TGTTTCACGC AGTGACGGCA TCGTCAAGCG CCATTTACGT TGTCAGGTCT 
GCGCTGAGCG TGTCGTGGCA CTCCGTGTTT GCGGTGTTGG GCAACACGTT TCATATGGCT 
GGCGGTGACG GTGCGCCGAT ACATCTTGAA GGGTCTAGCC AATCCTCATC GCTGAAGGTT 
CTGAACAACT CTGCCGTGGT GATCCGAGGC AACGTTGTGT CGAGGCCGGT GCAGTGCTTT 
GTGTTATTTA TTCGGACTTT AGACGTGGAG TCTCTTTCAG CTATTGTGTT TCAGGGGAAC 
GAGATGCAGG GAAGCTCGGT TGTGTTTTAT TCAGGCGAAT CGTCCAACAT CTACTACAAC 
TCCTGGTTGC AGTTGAGTGG CAACCTCTGC CGTGAATCCC CATCGGAAGC CCTTGCTTTT 
TTTTACTCCA CGGTGAATCT CCGCGACTCC ACGGTGTCTG TTTTTGGCAA CCAATTCATT 
TCCAGCACGG GAATGACGAA AGCGCTGTGG ATACATGCAG TTTCCAGCGA CCTCAAAAAC 
GGAGAGATTG TGGCTGCGTG CAACAGTGTG AACGGCGAGG AGGAGGCGAA CTACGCCATT 
CCGTCTGTGT ACAACGCCAC ATTTCTGACG TGCAGCGACC CATGCACCCT CGCGGCGTCG 
TGCTTCCCCG CGTACACGAC GACGGCGTCG AGTGATGGCT GCGCCTGCAC GTGCGCGGAG 
GGCGGCCATG GCGATGCATG CCTGCCTGTT GCTGTTCCCG AGCCACCGAG CACCGACGGA 
GCCGACTTGT GCGTGCGGGA CGTGCGTGTG GAGGTGGGGG TGAGCGCCGG TCTCGGGACG 
TCGGTGGCGT GCTACGTTGG CGTGACCTTT GCGGCGGACG TCGTGGTGGA TGTGGAGTCG 
ATGTCGGGGA GCGTGCGCAA CGTGACGCTG GCGAACTGTA CGTTTGTGGG CGGAGCGAGC 
CTGTACGTTT TGGGGTGGCG TTCCGACTCG CCTGCTGGCG AGCGCGCCGA TGTGTTGATC 
AGCGGGCTTG AGTCGCGCTC CGGCGGCGGC GTGTTGGTGG CGAACCGATT TCCGCCGGGC 
TCGCGCGTCA CTGTGGTGGA CTCGGTGCTG ATTGCAGAGA AGCGTGTTGC GTACCGCGAC 
GCCTACGACC TTGGCGACGT CTCCGCGTGC CTCGTGGTGC ACAGCGTGAA TCTGACTGGG 
AGCGTGCTGA CCATTGCGCG CACGCACGTG GCGGCTGTGT TCGGCGACGC TGTTGGCGTG 
TTGTTGGTTG GCGGCGTGGC GCTGCAGTCA CGTGGTGCGC TGTACGTGGA CGGACTGTCG 
GTGCAGACGG CGCTGGGGCT GTGCGTGTCG GTGGAGGGCG GTGTTTCGGC CAGCGGCGGC 
TCCGTTGTGG CGTTTGTTGA CAGCGACTTC CTGCTGTGCA AGCACGCGGT GTGTGTGCGT 
GGGGCTGTGA GCGTGTCGGG CTCCGCCGTG GCGCTTGTGC GGAGCGACTT TTCGTTGACG 
GAGGACTACG CGGTGGCGTT TTATTCGACG GTGAGCCTGG ATGGCGGGTC GATGCTGCTG 
GCGAAGGGCA ACGTGCACGA CGGCGTCTCG AGGGAGATGC TCCACGCCGC TGGCGCCGTG 
ACCGCTGCTG GGAGCACGCT GAGCTTTGTG CGCAACCGAG CGCTGCTGCC GCGGATGCTG 
TCGCTGAGCC TGTCGCTTGC GGCTGGGGCG CATGTGAGGG TGGCGTGCAA CTACGCGGGT 
GGACGTGTCC TGTCGACGGC GGAGGAGTAC GCTGCTGCTG GATTTGGCGA CGCTGGGCGC 
ATCGACGTTG CTGGGTGCGA CGCCTGCGAC AGGGACACGC ACTGCTACGC GCCCGGGACG 
GCGTCGGCCA GCATGAAGGA CGGTGTGTGC GAGTGCGTGT GCGGCAGCGG CGGGTACGGC 
GAGGCATGCG TGCCTGTGGG TGCTCCCGTG CTGCCGACCG CTGTGGGCAC TGCGTCGAGT 
TTGTTTGTCC GTGAGGGCGT GACGGTGCGG TCGGTGTTCG TTGTGCCTGC CGGCGCGAGC 
GAGGTGACGC TGCGCCACGT TGTGCTGGAC GGCGTGAGTC CGGTGCTCTA CGTGCCGTGG 
ATGGCGCGGG ACGGCGTGCG GATTGTTGTG CAGAACGTGT CGCTGCTGAA CGGCGCCGTG 
CTGTACGTGA TGGGCGGTGG AGGCCTGCGC GGTGCGGTGG CGGCGGGGAG CGACGAGGGC 
GGGCCGGTGG AGCTGTCGGT GTGCGATGTG GAGGCGCTGA ACGGTGCGCT TGTGCTGACC 
GGCACGTTCC CCGCGGGGTC CGTGCTGACG GTGACGGACT CCGTGCTGGT TGCCGCGAGG 
CCGACGCCGC TTGTGTATCT TCCCGGCTCG CGGTCCTCGC CGTACGCACC GGTGCTGGTG 
CTGTCGGGTC TGCGGCTCGT GCGGTCCGTG CTGGTTGTGT CCGGCGTTGC CCTCGTGACG 
GTGGTGACTG GTGGGCGGAC GGTGGTGGTG GACGGCGCCG TGCTGGAGCT TGTCGGTGGC 
GGTGTCGCGC TGGACGCGGC TGTGTTCGGT GGCGACTATG CGTTGTACGC GAGTGCGCGC 
GTGGTTGCGT CTGAGGGCGC TGTGCTGCGC GTGTCGGGGA GCCAGGTGTA CGCCGCGCAT 
GGATTGTTGT TCGAGAGCGG TGTGGAGGCC AACGCGTCCG CCGTCGTGGT GAACGACAAC 
GCCGGTGCGC TGACCGATGG CGCGCTGCTG GTGCTGCGGG GGTCGGCGTC GTTCGTGTCC 
GGGTCGTGGC TGTCGGTGCG CGGCAACAGC ATCAGCGGCA GGCTCCTGTC GCTGCCGTCG 
TACCCGCGCC GTGCGGAGCT CGTGCAGAGC ACGCTGACGC TTCACAAGAA CGCTGGCAGC 
GGACCCGTCG TGATGGACGG CACCGTTGCG CTCGGGGGTG CCGGCCGAAA GTTTGTCGTG 
GGGTGCCTGA CGCTGAACGG GCATGCGCTG CAGCCGATGG ACTACAGATC CGCCGGCATC 
ATCGGGAAAT TCCGCCCCGT GGCGTGTGGC GTTTGCGATG CCGACGTGCG CTGCTTTGCA 
GCTGCGACGA GGGCGATGTC GGGGTCGTGC GGCTGCCGCT GTGCTGAGGG CGGGTACGGG 
CGCGACTGCC TGCCGGTGTA CCTGCCGCAC GTGGACGGGT GCAACCGCAC GCCCGCCCTG 
CCGCTGCTGA GCCACACGGA GACGCTGACG GAGACGCGCA GCCTGACGCC GGGCATGAGT 
ACGACGCACT ACAGTCCGAC GCAGTACAGT TCGACGGAGA CCCTGCAGGT GACGGAGACG 
GTGGCGCTGT CGCCCACGCG GACGCCGACG GCATCGGTGA GCAGCACGCT GTGGTGGAGC 
GACGTGGCGT GCCCGACGCT TGCCGTGACG ACGACGGCGG CTGGTGGGAG CCTGACGCAG 
AACGACATTC GCGGCGGTGG GAGCGCCGTC CCGACGCGGC TGATGGTGGC GCTGCCGCCG 
CCGTTTCGGT GGGCACGCGA CCCGCAGCTT GGCACGCACC TGAGCTTCGT GCCGGTGTCG 
ACGGCGCAGC CGCGCGGGAT CGGTGGTCCG TGGGGAGCGA TGCTGAGTAA CGCGAAGTGG 
GTGCGCAACG CGACGAATCC GTCCACCGTT CTGGAGCTGG CTGTGCCCGT GCACCGCGGT 
TACTTCATTG CTGCCGACGA GACGATATTC ATTCGCTGCG ACGCTGTGGC GGTGTTCGGC 
GGCTGCAAGG GTGTGCTGCT TGGGTCCTTC ACGATCAGGT CAAACACGCT GCCCGCCGCT 
GCCAGCGTCG TCTCGGCGAT CACCGGCGTT GTTGCGGGTG CGGCGGCTGT TGCCGTGGTG 
GTGACCGGCG GACTGGGCTC CATTCTGGAG ATGCAGGCGC TCGGCGTGTT CGCGAGGATG 
CCATGCGCCT CGGCGCAGGA GCGTGCGAGC ACCGTTGCGC TGCCTTACTT CCTGTCGGTG 
TTCGCTGCCC GTGACCCGCT GTGGATGGTG GTGGGCAACG CGCTGCTCGC CGCTGTGTTC 
GGGTGCGTGC ACTGCGGTGT GACGGCGGCC TTTCAGCGGT GGCGCGGCGT GGACGCGGCG 
AGTGCGTGGG CTGCGATGCG CTTCCCGAGC CTGACGTACG TCGTGGCGCA CGCGATGCAC 
CTCGGCATCT TCTTTGGCTC CGTGCTTGCA CTGGCGATGC CCGACGCCCG CGTGCAGCAC 
CGCGTAATTG GTGTCGTTGG CGTGCTGTAC GGTGCGGCGT TCCCTGCTGG CGTGTGCTAC 
TTGATTGCCC GCCACGTGGG CGCGAGCTTC ACGAGTTACT GGCGGTTTTC GAGGAAGCCG 
CTGCACGAGC GCCTGCTGTA CCCCGTTGGG TATTGGCACC CCGCGGCGCA GCAGCGGATG 
TACGGCGGCA TGCTGACGAA CATGAGGGGC AGCTGCGTGT ACTGGTGCGT GTTCCAGCTG 
TCGGTGTTGT GCGTGGTGTG CCTGATTGCG GCTGTGCACC CGCCCGTGGG AGGCTGCCAC 
GTCCAGTATT TCTGCACGGC GGCTGTGCTG CTTGCGGGTG CGGGCGTGAT TGCCTTCACG 
AACATGATGC GCTCGGCCTT CCTGACGGTG ATGCACACTG CGGGCTTTGT ACTCCTTGCG 
GCGCTGTGCG TGGTCAGCGC GGCCAACCAC CTTGCGCCGA GCGACGGCGG TGCGAGGGCG 
TACGCGGCTA TCGTGCTGCT GCTGACGACT GTGCCGCTTT TGGCGACTGT GTACAGCGTC 
GTTGTGTGGT ATGCGGAGGA CCGCCACTGG CAGGAGCTGC GCGAGCCGCG GCGTGGAGGG 
CTGGAGGCGC TGCTGCGCGA TGACGAGGAT GGCGACGAGG AGACGCAGAA GCCCCACGAA 
ATGACGTCGT CGTACGCCTC AGGCACCACC GTTGCGTCGT TGTACCGACC ACCCGCACCG 
CCGTTGCAAC CCATGGCGGT GACACCCACT CCGACACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053506241.19 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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