Tb927.7.7200 cDNA ORF clone, Trypanosoma brucei brucei TREU927

Gene
Clones
gRNAs

Summary

Gene Symbol	Tb927.7.7200
Entrez Gene ID	3658948
Full Name	hypothetical protein
Synonyms	Tb07.21H15.130
Gene Type	protein-coding
Organism	Trypanosoma brucei brucei TREU927

mRNA and Protein(s)

mRNA	Protein	Name
XM_841247.1	XP_846340.1	hypothetical protein, conserved

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

The genome of the African trypanosome Trypanosoma brucei.
Berriman M, Ghedin E, Hertz-Fowler C, Blandin G, Renauld H, Bartholomeu DC, Lennard NJ, Caler E, Hamlin NE, Haas B, Böhme U, Hannick L, Aslett MA, Shallom J, Marcello L, Hou L, Wickstead B, Alsmark UC, Arrowsmith C, Atkin RJ, Barron AJ, Bringaud F, Brooks K, Carrington M, Cherevach I, Chillingworth TJ, Churcher C, Clark LN, Corton CH, Cronin A, Davies RM, Doggett J, Djikeng A, Feldblyum T, Field MC, Fraser A, Goodhead I, Hance Z, Harper D, Harris BR, Hauser H, Hostetler J, Ivens A, Jagels K, Johnson D, Johnson J, Jones K, Kerhornou AX, Koo H, Larke N, Landfear S, Larkin C, Leech V, Line A, Lord A, Macleod A, Mooney PJ, Moule S, Martin DM, Morgan GW, Mungall K, Norbertczak H, Ormond D, Pai G, Peacock CS, Peterson J, Quail MA, Rabbinowitsch E, Rajandream MA, Reitter C, Salzberg SL, Sanders M, Schobel S, Sharp S, Simmonds M, Simpson AJ, Tallon L, Turner CM, Tait A, Tivey AR, Van Aken S, Walker D, Wanless D, Wang S, White B, White O, Whitehead S, Woodward J, Wortman J, Adams MD, Embley TM, Gull K, Ullu E, Barry JD, Fairlamb AH, Opperdoes F, Barrell BG, Donelson JE, Hall N, Fraser CM, Melville SE, El-Sayed NM
Science (New York, N.Y.)309(5733)416-22(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tb927.7.7200 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tb927.7.7200 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr134272	XM_841247.1 Latest version!	Trypanosoma brucei brucei TREU927 hypothetical protein, conserved (Tb927.7.7200), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tb927.7.7200 ( XM_841247.1 ) cDNA ORF clone, Trypanosoma brucei brucei TREU927 -> XP_846340.1 Trypanosoma brucei brucei TREU927 hypothetical protein, conserved (Tb927.7.7200), partial mRNA.

Price & Availability↑

CloneID	OTr134272
Clone ID Related Accession (Same CDS sequence)	XM_841247.1
Accession Version	XM_841247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523203200000
Organism	Trypanosoma brucei brucei TREU927
Product	hypothetical protein, conserved
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007280). COMPLETENESS: incomplete on both ends.

ATGGATACGT ACAGTAAGTT GTCGCTAGTG AAGGAGAATG ATGTCGATGT GAACGATGTC 
CAAGCGCAAG TAGATGTTAT GTACGCGAGT ATTTTGGCCT CCGTCGATAT GATAATGGAG 
AAGGAAGCGG CTGCAGCCAG AAAAGGGGCT CCCACGCGCG GGGCAGCTCA CCACTTCAAG 
CAGCGAGGTA CGCAGGCTGC GTCCTCTTGC GCATCAGCGG CACCACCAAA ACCAGAGACA 
AGATGCGCAC TTCCAGCAAC GGAGACGAAG GAGCCTGACG AAGCTGCAGA GACTTGCGCA 
TCTCAATGTG GGTGCTCGTG TGCTCACGAG TGCACGTCCA TGGGCCCCAA GGCGTCTTCC 
GGATGCCAGC ACGTTCGTCG TCACAACTCC CAGTTGGCTG CTACAAAGTG CCGTCGGCCT 
CATGAGGCAT CAGCGGAGAA AGTGAAGATA CCTTATAGCA CTCCGACTGG TCGGGTGGTT 
AATGCTGCTA CGGTCACTAG AAGTTCACAG CATCGCTTCC CCCCATCTAC ATTCGGAAGT 
GGTCGGAAGG GGATGTCTGA ACCGATACGA AAAGATACGT CACCAGGGCC CGGATCGTAC 
TTCATTGCGG ATGACAGCAA TAACGACAGT GGGGACTGCT ACCGCGGACG GGGTGCTGAA 
GCTGGGCATA GTAAGAAAAG TCACACAAGC GGTCCACATC ACACGGGGGT TGAGGTGGTG 
GAACAGAAGG CGGAGAAGAA TGAGCTACTC AAGAGACAAA TGGAAATACT CTGTCAGTGT 
TATTGGAAGC AAATGCCTGA TATCGACGCA CCGAAGAATC CCGCGGCTCA CGCGTCCGAC 
TGCCCTTGTC AGTCACTGAG AACAGTGTGT ACAGAGGGGG ACAATGACGA TCACACCAGC 
AGCCGCAGCA ACTCCGGCAG TGAGGCTGCT CAACCTTGCG GCAGCAGCAC AAAGAAAAAT 
AGCAACAAAA AAAGCGGTGC GACAAAGGCA CATGATAGTT TTCAGAAATC TCCCTCGGTG 
GTGTCGCCGC GGCTTGTAGG ACGGCTCTCC AATCGGGGTG CCGTATTCGG AATTTCACCT 
CGGTGCACTA TTTTCGCTGT AGACACATCT GACACTAATA CACATCCGAA GCACCGCTCC 
AGTCAACGAA GGCGCGGCAG AAGTGAGGAG GAAGTCCGAA GCGGTCAGCG GGCAGTGGGA 
GACTCAGACG TACATGGCAG CGTGCCTTCA TACTTGAAAC CAACAAAACG AGTTGATGGT 
GCCTCGGCGG GGGTGTCACC GGGTCCTGGG GCGTACGATG TGTCTGGTGA ATTAGGTATG 
TCTGCATTGT CCCCCGGGAA AACGGCCACT GGGGGGGTGC TAATTGGACG GGCAGAACGA 
AAGTTGCATG CTGTCAATAA TTTTCCCGGG CCCGGAGCGT ACGACGTCAA GGAAAAGAGT 
ATGACAGAAA CCTCATCAAA GAAAAGTGCC CGCACCCTAT CGAGTAGTCG TCGTCAGCTT 
TCTATACTTC CCACAAAGGA GGCGGTGCTC ATACCAGGAC CTGGCGAATA TCACCCAGAA 
GCCGCGACAC TCTCCGACGC GCGGCGGAGG AGCCCTCCTG CTTTCTCATT TGGTCGTGCG 
AAGTTACTAC GTTATGGACG AGTGCCACTC ACTTCTTCAC CACCTTCTGA ACCAGCGAAC 
GTACCAGGTC CAGGCACGTA CGATACCGCT TCTGTGCCAA GCGACAGGTG CACAGCCGTC 
AGTGGTCGCA GTGCCGTGAT AACGAACGTA AGACGTGGTG GATCTCCGTG GAGCAAGGAG 
CGCACTTCCC CTCATGAAGT ACCTGGACCT GGGGCATATG AACCCGTCAG GAGGGAAAGT 
GGTCCAGCCT ACCTTATGGG ACGCCGACCA GCAGAGCGGG TGGATACAAC GCCTGGTCCC 
GGCCACTACG AATTGCCACA AATGTCTACC GGTCCACGGT GGCTTGTGTC GGAGTCGCCC 
TCGGCACCTC TCGAGCCCCA TCCTACCCCA GGACCTGGTG AGTACAGTGT TTCCACATAT 
GAGCTAAAAC ATTCGCATTC CTCTGCGCCC TTATTCCCTA CAGCGCCGCG TGTGCTGCAC 
GAAGGGACGA AAGTTGCTGA GACGCTCCCC GGACCGGGAA CTTACGAGAC AGAGAGACAG 
ACCAGTCCCG TGCGGTCTGC CATCATGGGT ACCGCACAAC GAGTATCAAC GCTTGGCTTC 
GACTGCTGTG CTGATGTGCC GGGGCCAGGC ACTTACACAG CACTTACCAC TGTACAGGAG 
CCACGGGTCG TGTCGCCTCT ATTAAATGCC GCCGCACCGC GATTTGCGGA GAGTAGCGAA 
CCGGGTGCTG AAGGGGCTGC CGGTGTGGTG GCCACCCCCG GACCAGGACA CTACGATGCT 
GATTGTGGGG ATGTGGGAAC GAGGGGCCGT GCCGCTGTCT TCGGAAGTGC CCCACGAGAA 
TTACCATTCA ACGAGGATGA AGCTGCAAAA AAACCAGGCC CGGGTGCGTA CGAGATCGCC 
ATGCCGAAGA ATAGCCCGTC TTTTTCAATC GGTCAGAAAC TTGACGGCCC AGAGCCCAGT 
GTGTCAGTTC CCGGACCCGG AGAATACGAT CCACAAGATC CGATGCCGAA CACCCGGGCC 
GTTGTGATTG GTGAAGCTGC AAGGTTTCAG ACTAGCGGTG GGGAAAAAGA TCGTCAAGAT 
GTCCCAGGAC CAGGGGCCTA CACAATAGAG AGACCTCCGG AGTACAGGGG GGCGGCAGTG 
TTTGGCACAG AGCCACGCCT TTTAACCATC GACCACTCCG TGGGGAACCA CGATGGCAAC 
GACGGGATTG GACCAGGTTC ATACGATCCC CAAGTGCCTA AAGGTCCTGC ATCGGGGGCA 
GTGTGGAGCG CCGGTCCCAG GTTTGCTGCC GATACACTTA ATGAGGTACC GGGTCCTGGA 
GCATATGACA CTCGGCAGAG CCCAACCCCA ACTAATGTCG TGAACTTCGG CATATCTTGT 
GGCAAACCGT CCCAGCGGGC TGATCCCGCA GAGGCTGTCC CTGGTCCTGG GGCGTATGCT 
CCAAAAGACC CACATGGACG CACGATCCCA GGTGTAGTCT TTGGTTCGTC GCCGCAACAC 
CCGACATCAC CAGAAGAGCG TGAAGCGCTT ATGCGACCCG GTGTCGGTCC AGGATCATAC 
GAAGTTATCC TTCCCGTCCC GCACACCTTT CACACCACGT TCGGTAGCGC CGCGGATGGA 
CGAATACAGG AGGAGCGGCA CGGCGAGTTG GCGGTTGGTC CGGGTCACTA CAATCCCCAC 
GATAGCAAGG CCTCTTGTGC GCGCTCCGCC ATAATTGGCA TGGCACCTCG TCTACAGTCT 
CATGAAGACA GTTGGAGCAT CCCGGGACCC GGAGCGTATT CGTTAGACCC GACCACTGGA 
GGCGCGTCAC CAGTCGGTCC GGTCTCCTTC ACAACAGCAC CCCGGTTCGA CAATGTTGCG 
GGGGCTGCTG CAGGTGGTGA CAACGACGTC ACTCCGGGAC CTGGATACTA TTCAATAGAA 
GATCGTGGAA CCCTCACAGC GGCTGCTGGT GTTCCTGTGT TCAGCAGGGG GCCACGTGTC 
CTCGATCTGA AGGAACGAGA ACACGCACTT CTTCCCGGGC CTGGCCAATA TCATCCGGAG 
GACCTAAGCA CCAAGGTAGG TGGAGCAGCC TACTCTTTTG GAGTGGAACC TCGCATGCCA 
CAGGATGCTA CCGAGAACGA TGGAACCACA CCCGGCCCCG GAGCCTACGA TGTCACCATC 
CCCACACTCA CAGCTACAGG CACACGGTTC GGTGTCGAAC CACGTGCCTT GGGTTCTTTG 
AACCATGAGG AAGGGCCGGG ACCAGGAACG TATAATGTTG GGGCGTCGCG TACAACGTCA 
CCGACGGCAC CTGTCTATCG CTTCCCCACA GCTACCCGGG AGGCGCCCAG TCTTGAGGGA 
AGCGACGTCC CTGGGCCTGG GCAGTACGAC TACACTGGGG AAGATTTATC TCATCGCCGA 
GCCGTTGTTT CCGCCTTTCC CATAAGTCCT CGCTTTGCAA CCGAGAGAGG GGAGGACGAA 
GCCCCTGGAC CCGGGCAGTA CATGCCCCAC CATCCGCAAT GGGACTCTTC CCAAGCATAC 
TCTTTCCCCA TGGGAGGAAG AAACGCTTCG CTCACTCTAG GAACGGATGG GCCCGGACCA 
GGAACATATA GCCCACAAGA ACCCTCACTA ACGGCAACAG CAGCTCTCTT TGGTATTGGG 
CCGCGCCAAA CAAGCGGAGG TTTGAACAAC CTCAGTGGAC AAAACCATCC GGGACCTGGA 
CACTACAACC CTGCGCTGCC GACGGATACA CCTTCCGTTA TTTTTGGCAC CGCACAAAGG 
GACGTAGCAA ACACGGGTGG CATTGAAGAT GCCCCAGGGC CAGGTTCTTA CGACCTAAGG 
GTGGAAAACC GTGAGGGGAT AGCGTATTCC ATCGGACGGG CTGCTGCCCA GGATCGCATG 
GCGGTTGATA TGCAAAAAGC CGAACTTCCA GGCCCTGGCA CGTACTCGGT GCAGCCCGCA 
TTTCCTGCGG TCGATGGTGG GAGCGGGGCC ACTTCATTTG TCAAGGCTGC GCGGTTCCCA 
GATGGCCAAG CTGCCCAAAC CGAAGGACCT GGGCCCGGTG CATACAGCAT TCAATCGGCG 
CAACGGACCT CAGGGCCCTT TATTGGTCGA GCGCCCCGTG TTGCCACAGG GGTTGGTGCC 
AACAATAACG ATCTCAGAGA ACAGCCAGGA CCAGGTCACT ACGAGGTCAG CAGTGGAGGG 
CTTGGGAGTA TTGTGGACGG TGGCGTAGCG ATGCGTTTCA CGAGCGAGCG CTTCCCTGGG 
CTCAGCGGTG GCGCAGTGGG AGAGAGTCCA GGTCCGGCGT CGTACAGTCC CGCAGAGGCC 
AGAGGTACGA AGGATGGTGT GTCGTTCCCA CAGGCACCGC GCCATTCGCC GGCAGAGCTG 
AATGATGCTT CATCGCGTCC TGGACCTGGT GATTATGATA TCCCCTCTAC CTGGAACACC 
ACAGCGGGCA ACACCTATTC CTTTGGTGCT GAAGAGCGCA TGAAAGCCCC CACGGTAGAT 
GCCGATGTAC CGGGGCCCGG TAGCTACGAT ACGAACCGTT ACCTGAACGC ATATCAAGCT 
GGGCCCGCAT TCACTCTTAA AAGTAAACCA GGACAGAATG AAAACGGTGG TGACCTTCCT 
GGTCCTGGGA GCTACGATGC AAGTCGCTAC ATAGATGAAA GTAGGGCGGG TCGTGCGTTT 
ACTCTTCGAG GGAAAAGTAC ATTTAACGGC AGCAGTGGCA ATGGTGATGT TCCTGGTCCG 
GGAAGCTACG ACCCAAATCG CTATCTCGAA CAATGCCAAG CCGGCCCGTC GGTCTCATTG 
AGAGGGAAAG CAGCCCCCGT CGGCGGACAT GATGCTGACG TCCCCGGTCC CGGAAGCTAT 
GACGCGAGTA GCTACTTGGA AAGGTGCCAA ATGGGCCCCG CCTTCTCGTT GAAGGGAAAA 
CCAACCTGCG TTGACGAAAA CAGTGCTGGC ATTCCCGGCC CCGGTAGTTA TGATGCGAAC 
CGATACTTGG AGCAGTTTCA GTCAGGACCC GCGTTCACAC TTCGAGGAAA AGGTGCGTGT 
AATGGCAATG ACAGCGAAGC TGACGTTCCA GGTCCGGGGA GCTACGATCC GAATCGTTAC 
CTCGAACAAC ACCACGCAGG GCCGTCGTTT ACCCTAAAGG GCAAGGCCAC GTATAATGGC 
GAGAATGGTA TTGATGCTCC GGGACCTGGC AGTTATGATA TCAATCGCTG TATGGAACAG 
TCTCAGGCAG GTCCTTCATT CACGTTAAGG GGGAAACCAA GCGACACTGG GCAGAAGGGG 
CCTGATATCC CTGGTCCTGG AAGCTATGAT GCCAGTCAGT ACTTGGAGCG GTGTCAAGCG 
GGTCCTGCCT TCACTCTCCG AGGGAGGGAT GCGTTTAACA GTAACAGTGC CGATGGCGAC 
GTTCCTGGGC CGGGAAGCTA CGATGTGAAT CGATATTTGA AGCAGTGTCA GTCGGGGGTT 
TCATTTACTT TGAAGGGAAA ATCAGCGCAA AACGACAACG AAGCTGACGC TCCAGGTCCG 
GGGAGCTACG ATCCGAATCG TTACCTCGAA CAACACCACG CAGGGCCGTC GTTTACCCTA 
AAGGGCAAGG CCACGTATAA TGGCGAGAAT GGTATTGATG CTCCGGGACC TGGCAGTTAC 
GATATCAATC GCTACATGGA ACTGTGTCAG GCGGGCCCAG CCTTTACTTT GAAAGGAAAA 
GCATCCCAAG TTGAAGGTAG CGCCGATGTA CCTGGCCCCG GTAGCTACGA CGTTAGCCGC 
TATACGGAAC AATTTCAGTC GGGCCCTGCC TTCACGCTCA AAGGAAAACA AGTGACCAGC 
GACGGGGTCG ACGCCGACGT CCCCGGTCCC GGAAGCTACA ACGTAAGCAG CTACGCAGAA 
CAGTTTAAAC CGGGACCAGC TTTTACGGTG AAGGGGAAGC CATCGGGCGC TGCCGAGAAC 
AGTGATGGTT TACCTGGGCC CGGAAGTTAC GACACGGATC GATACATGCA GCAATGCCAT 
TCAGGTCCCG CCTTCACTCT GAAAGGGAAA CCAACGTACG GCGATGGCCG CCAAACGGAA 
GATGTCCCTG GCCCCGGTGC CTATTGTCCA CGACCTACCG AAACAACTCG TGCAGTTTCC 
TTCGGTCCAT TGCCCACCGT TGATTCGACA GCCGTATTTG AAGTGCGGGT GAACGCAACA 
CCCGGGCCTG CAGCGTACGT TATCCCTTCC ACACTAAACC AAAAGGCCGT ACCGTTTGGG 
GCAGCGGAGC GGGAGGTGCA GTGCAGAGCA GAAGATGTTC CAGGACCGGG GGCATATCTA 
ACAGGTGGCC CAGTCGTCTT AAAACCCGGC CCCGCATTTA CCTTCGGCAG GGGGCCACGC 
TCTATGACGC TGGAATCTGC CCAGGAGGAC GCACCCGGTC CCGGTGAATA TCACCACCCC 
GTTGACGCAA GACCCACCGT ACTTGCCTTC ACATTCGCTT CTGCCGATCG CTTCGCTGAG 
TTTGAAGCAG GCAATGGCGT GGGTCCCGGT GAATATTACC ACCCGTACCC TACCGAGACC 
CACGGTGGTC CGGCCTTCAG ATTCCCCAAA TCTATGCGTG CTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_846340.1
CDS	1..7188
Translation	MDTYSKLSLVKENDVDVNDVQAQVDVMYASILASVDMIMEKEAAAARKGAPTRGAAHHFKQRGTQAASSCASAAPPKPETRCALPATETKEPDEAAETCASQCGCSCAHECTSMGPKASSGCQHVRRHNSQLAATKCRRPHEASAEKVKIPYSTPTGRVVNAATVTRSSQHRFPPSTFGSGRKGMSEPIRKDTSPGPGSYFIADDSNNDSGDCYRGRGAEAGHSKKSHTSGPHHTGVEVVEQKAEKNELLKRQMEILCQCYWKQMPDIDAPKNPAAHASDCPCQSLRTVCTEGDNDDHTSSRSNSGSEAAQPCGSSTKKNSNKKSGATKAHDSFQKSPSVVSPRLVGRLSNRGAVFGISPRCTIFAVDTSDTNTHPKHRSSQRRRGRSEEEVRSGQRAVGDSDVHGSVPSYLKPTKRVDGASAGVSPGPGAYDVSGELGMSALSPGKTATGGVLIGRAERKLHAVNNFPGPGAYDVKEKSMTETSSKKSARTLSSSRRQLSILPTKEAVLIPGPGEYHPEAATLSDARRRSPPAFSFGRAKLLRYGRVPLTSSPPSEPANVPGPGTYDTASVPSDRCTAVSGRSAVITNVRRGGSPWSKERTSPHEVPGPGAYEPVRRESGPAYLMGRRPAERVDTTPGPGHYELPQMSTGPRWLVSESPSAPLEPHPTPGPGEYSVSTYELKHSHSSAPLFPTAPRVLHEGTKVAETLPGPGTYETERQTSPVRSAIMGTAQRVSTLGFDCCADVPGPGTYTALTTVQEPRVVSPLLNAAAPRFAESSEPGAEGAAGVVATPGPGHYDADCGDVGTRGRAAVFGSAPRELPFNEDEAAKKPGPGAYEIAMPKNSPSFSIGQKLDGPEPSVSVPGPGEYDPQDPMPNTRAVVIGEAARFQTSGGEKDRQDVPGPGAYTIERPPEYRGAAVFGTEPRLLTIDHSVGNHDGNDGIGPGSYDPQVPKGPASGAVWSAGPRFAADTLNEVPGPGAYDTRQSPTPTNVVNFGISCGKPSQRADPAEAVPGPGAYAPKDPHGRTIPGVVFGSSPQHPTSPEEREALMRPGVGPGSYEVILPVPHTFHTTFGSAADGRIQEERHGELAVGPGHYNPHDSKASCARSAIIGMAPRLQSHEDSWSIPGPGAYSLDPTTGGASPVGPVSFTTAPRFDNVAGAAAGGDNDVTPGPGYYSIEDRGTLTAAAGVPVFSRGPRVLDLKEREHALLPGPGQYHPEDLSTKVGGAAYSFGVEPRMPQDATENDGTTPGPGAYDVTIPTLTATGTRFGVEPRALGSLNHEEGPGPGTYNVGASRTTSPTAPVYRFPTATREAPSLEGSDVPGPGQYDYTGEDLSHRRAVVSAFPISPRFATERGEDEAPGPGQYMPHHPQWDSSQAYSFPMGGRNASLTLGTDGPGPGTYSPQEPSLTATAALFGIGPRQTSGGLNNLSGQNHPGPGHYNPALPTDTPSVIFGTAQRDVANTGGIEDAPGPGSYDLRVENREGIAYSIGRAAAQDRMAVDMQKAELPGPGTYSVQPAFPAVDGGSGATSFVKAARFPDGQAAQTEGPGPGAYSIQSAQRTSGPFIGRAPRVATGVGANNNDLREQPGPGHYEVSSGGLGSIVDGGVAMRFTSERFPGLSGGAVGESPGPASYSPAEARGTKDGVSFPQAPRHSPAELNDASSRPGPGDYDIPSTWNTTAGNTYSFGAEERMKAPTVDADVPGPGSYDTNRYLNAYQAGPAFTLKSKPGQNENGGDLPGPGSYDASRYIDESRAGRAFTLRGKSTFNGSSGNGDVPGPGSYDPNRYLEQCQAGPSVSLRGKAAPVGGHDADVPGPGSYDASSYLERCQMGPAFSLKGKPTCVDENSAGIPGPGSYDANRYLEQFQSGPAFTLRGKGACNGNDSEADVPGPGSYDPNRYLEQHHAGPSFTLKGKATYNGENGIDAPGPGSYDINRCMEQSQAGPSFTLRGKPSDTGQKGPDIPGPGSYDASQYLERCQAGPAFTLRGRDAFNSNSADGDVPGPGSYDVNRYLKQCQSGVSFTLKGKSAQNDNEADAPGPGSYDPNRYLEQHHAGPSFTLKGKATYNGENGIDAPGPGSYDINRYMELCQAGPAFTLKGKASQVEGSADVPGPGSYDVSRYTEQFQSGPAFTLKGKQVTSDGVDADVPGPGSYNVSSYAEQFKPGPAFTVKGKPSGAAENSDGLPGPGSYDTDRYMQQCHSGPAFTLKGKPTYGDGRQTEDVPGPGAYCPRPTETTRAVSFGPLPTVDSTAVFEVRVNATPGPAAYVIPSTLNQKAVPFGAAEREVQCRAEDVPGPGAYLTGGPVVLKPGPAFTFGRGPRSMTLESAQEDAPGPGEYHHPVDARPTVLAFTFASADRFAEFEAGNGVGPGEYYHPYPTETHGGPAFRFPKSMRAA

Target ORF information:

RefSeq Version	XM_841247.1
Organism	Trypanosoma brucei brucei TREU927
Definition	Trypanosoma brucei brucei TREU927 hypothetical protein, conserved (Tb927.7.7200), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_841247.1

ORF Insert Sequence:

ATGGATACGT ACAGTAAGTT GTCGCTAGTG AAGGAGAATG ATGTCGATGT GAACGATGTC 
CAAGCGCAAG TAGATGTTAT GTACGCGAGT ATTTTGGCCT CCGTCGATAT GATAATGGAG 
AAGGAAGCGG CTGCAGCCAG AAAAGGGGCT CCCACGCGCG GGGCAGCTCA CCACTTCAAG 
CAGCGAGGTA CGCAGGCTGC GTCCTCTTGC GCATCAGCGG CACCACCAAA ACCAGAGACA 
AGATGCGCAC TTCCAGCAAC GGAGACGAAG GAGCCTGACG AAGCTGCAGA GACTTGCGCA 
TCTCAATGTG GGTGCTCGTG TGCTCACGAG TGCACGTCCA TGGGCCCCAA GGCGTCTTCC 
GGATGCCAGC ACGTTCGTCG TCACAACTCC CAGTTGGCTG CTACAAAGTG CCGTCGGCCT 
CATGAGGCAT CAGCGGAGAA AGTGAAGATA CCTTATAGCA CTCCGACTGG TCGGGTGGTT 
AATGCTGCTA CGGTCACTAG AAGTTCACAG CATCGCTTCC CCCCATCTAC ATTCGGAAGT 
GGTCGGAAGG GGATGTCTGA ACCGATACGA AAAGATACGT CACCAGGGCC CGGATCGTAC 
TTCATTGCGG ATGACAGCAA TAACGACAGT GGGGACTGCT ACCGCGGACG GGGTGCTGAA 
GCTGGGCATA GTAAGAAAAG TCACACAAGC GGTCCACATC ACACGGGGGT TGAGGTGGTG 
GAACAGAAGG CGGAGAAGAA TGAGCTACTC AAGAGACAAA TGGAAATACT CTGTCAGTGT 
TATTGGAAGC AAATGCCTGA TATCGACGCA CCGAAGAATC CCGCGGCTCA CGCGTCCGAC 
TGCCCTTGTC AGTCACTGAG AACAGTGTGT ACAGAGGGGG ACAATGACGA TCACACCAGC 
AGCCGCAGCA ACTCCGGCAG TGAGGCTGCT CAACCTTGCG GCAGCAGCAC AAAGAAAAAT 
AGCAACAAAA AAAGCGGTGC GACAAAGGCA CATGATAGTT TTCAGAAATC TCCCTCGGTG 
GTGTCGCCGC GGCTTGTAGG ACGGCTCTCC AATCGGGGTG CCGTATTCGG AATTTCACCT 
CGGTGCACTA TTTTCGCTGT AGACACATCT GACACTAATA CACATCCGAA GCACCGCTCC 
AGTCAACGAA GGCGCGGCAG AAGTGAGGAG GAAGTCCGAA GCGGTCAGCG GGCAGTGGGA 
GACTCAGACG TACATGGCAG CGTGCCTTCA TACTTGAAAC CAACAAAACG AGTTGATGGT 
GCCTCGGCGG GGGTGTCACC GGGTCCTGGG GCGTACGATG TGTCTGGTGA ATTAGGTATG 
TCTGCATTGT CCCCCGGGAA AACGGCCACT GGGGGGGTGC TAATTGGACG GGCAGAACGA 
AAGTTGCATG CTGTCAATAA TTTTCCCGGG CCCGGAGCGT ACGACGTCAA GGAAAAGAGT 
ATGACAGAAA CCTCATCAAA GAAAAGTGCC CGCACCCTAT CGAGTAGTCG TCGTCAGCTT 
TCTATACTTC CCACAAAGGA GGCGGTGCTC ATACCAGGAC CTGGCGAATA TCACCCAGAA 
GCCGCGACAC TCTCCGACGC GCGGCGGAGG AGCCCTCCTG CTTTCTCATT TGGTCGTGCG 
AAGTTACTAC GTTATGGACG AGTGCCACTC ACTTCTTCAC CACCTTCTGA ACCAGCGAAC 
GTACCAGGTC CAGGCACGTA CGATACCGCT TCTGTGCCAA GCGACAGGTG CACAGCCGTC 
AGTGGTCGCA GTGCCGTGAT AACGAACGTA AGACGTGGTG GATCTCCGTG GAGCAAGGAG 
CGCACTTCCC CTCATGAAGT ACCTGGACCT GGGGCATATG AACCCGTCAG GAGGGAAAGT 
GGTCCAGCCT ACCTTATGGG ACGCCGACCA GCAGAGCGGG TGGATACAAC GCCTGGTCCC 
GGCCACTACG AATTGCCACA AATGTCTACC GGTCCACGGT GGCTTGTGTC GGAGTCGCCC 
TCGGCACCTC TCGAGCCCCA TCCTACCCCA GGACCTGGTG AGTACAGTGT TTCCACATAT 
GAGCTAAAAC ATTCGCATTC CTCTGCGCCC TTATTCCCTA CAGCGCCGCG TGTGCTGCAC 
GAAGGGACGA AAGTTGCTGA GACGCTCCCC GGACCGGGAA CTTACGAGAC AGAGAGACAG 
ACCAGTCCCG TGCGGTCTGC CATCATGGGT ACCGCACAAC GAGTATCAAC GCTTGGCTTC 
GACTGCTGTG CTGATGTGCC GGGGCCAGGC ACTTACACAG CACTTACCAC TGTACAGGAG 
CCACGGGTCG TGTCGCCTCT ATTAAATGCC GCCGCACCGC GATTTGCGGA GAGTAGCGAA 
CCGGGTGCTG AAGGGGCTGC CGGTGTGGTG GCCACCCCCG GACCAGGACA CTACGATGCT 
GATTGTGGGG ATGTGGGAAC GAGGGGCCGT GCCGCTGTCT TCGGAAGTGC CCCACGAGAA 
TTACCATTCA ACGAGGATGA AGCTGCAAAA AAACCAGGCC CGGGTGCGTA CGAGATCGCC 
ATGCCGAAGA ATAGCCCGTC TTTTTCAATC GGTCAGAAAC TTGACGGCCC AGAGCCCAGT 
GTGTCAGTTC CCGGACCCGG AGAATACGAT CCACAAGATC CGATGCCGAA CACCCGGGCC 
GTTGTGATTG GTGAAGCTGC AAGGTTTCAG ACTAGCGGTG GGGAAAAAGA TCGTCAAGAT 
GTCCCAGGAC CAGGGGCCTA CACAATAGAG AGACCTCCGG AGTACAGGGG GGCGGCAGTG 
TTTGGCACAG AGCCACGCCT TTTAACCATC GACCACTCCG TGGGGAACCA CGATGGCAAC 
GACGGGATTG GACCAGGTTC ATACGATCCC CAAGTGCCTA AAGGTCCTGC ATCGGGGGCA 
GTGTGGAGCG CCGGTCCCAG GTTTGCTGCC GATACACTTA ATGAGGTACC GGGTCCTGGA 
GCATATGACA CTCGGCAGAG CCCAACCCCA ACTAATGTCG TGAACTTCGG CATATCTTGT 
GGCAAACCGT CCCAGCGGGC TGATCCCGCA GAGGCTGTCC CTGGTCCTGG GGCGTATGCT 
CCAAAAGACC CACATGGACG CACGATCCCA GGTGTAGTCT TTGGTTCGTC GCCGCAACAC 
CCGACATCAC CAGAAGAGCG TGAAGCGCTT ATGCGACCCG GTGTCGGTCC AGGATCATAC 
GAAGTTATCC TTCCCGTCCC GCACACCTTT CACACCACGT TCGGTAGCGC CGCGGATGGA 
CGAATACAGG AGGAGCGGCA CGGCGAGTTG GCGGTTGGTC CGGGTCACTA CAATCCCCAC 
GATAGCAAGG CCTCTTGTGC GCGCTCCGCC ATAATTGGCA TGGCACCTCG TCTACAGTCT 
CATGAAGACA GTTGGAGCAT CCCGGGACCC GGAGCGTATT CGTTAGACCC GACCACTGGA 
GGCGCGTCAC CAGTCGGTCC GGTCTCCTTC ACAACAGCAC CCCGGTTCGA CAATGTTGCG 
GGGGCTGCTG CAGGTGGTGA CAACGACGTC ACTCCGGGAC CTGGATACTA TTCAATAGAA 
GATCGTGGAA CCCTCACAGC GGCTGCTGGT GTTCCTGTGT TCAGCAGGGG GCCACGTGTC 
CTCGATCTGA AGGAACGAGA ACACGCACTT CTTCCCGGGC CTGGCCAATA TCATCCGGAG 
GACCTAAGCA CCAAGGTAGG TGGAGCAGCC TACTCTTTTG GAGTGGAACC TCGCATGCCA 
CAGGATGCTA CCGAGAACGA TGGAACCACA CCCGGCCCCG GAGCCTACGA TGTCACCATC 
CCCACACTCA CAGCTACAGG CACACGGTTC GGTGTCGAAC CACGTGCCTT GGGTTCTTTG 
AACCATGAGG AAGGGCCGGG ACCAGGAACG TATAATGTTG GGGCGTCGCG TACAACGTCA 
CCGACGGCAC CTGTCTATCG CTTCCCCACA GCTACCCGGG AGGCGCCCAG TCTTGAGGGA 
AGCGACGTCC CTGGGCCTGG GCAGTACGAC TACACTGGGG AAGATTTATC TCATCGCCGA 
GCCGTTGTTT CCGCCTTTCC CATAAGTCCT CGCTTTGCAA CCGAGAGAGG GGAGGACGAA 
GCCCCTGGAC CCGGGCAGTA CATGCCCCAC CATCCGCAAT GGGACTCTTC CCAAGCATAC 
TCTTTCCCCA TGGGAGGAAG AAACGCTTCG CTCACTCTAG GAACGGATGG GCCCGGACCA 
GGAACATATA GCCCACAAGA ACCCTCACTA ACGGCAACAG CAGCTCTCTT TGGTATTGGG 
CCGCGCCAAA CAAGCGGAGG TTTGAACAAC CTCAGTGGAC AAAACCATCC GGGACCTGGA 
CACTACAACC CTGCGCTGCC GACGGATACA CCTTCCGTTA TTTTTGGCAC CGCACAAAGG 
GACGTAGCAA ACACGGGTGG CATTGAAGAT GCCCCAGGGC CAGGTTCTTA CGACCTAAGG 
GTGGAAAACC GTGAGGGGAT AGCGTATTCC ATCGGACGGG CTGCTGCCCA GGATCGCATG 
GCGGTTGATA TGCAAAAAGC CGAACTTCCA GGCCCTGGCA CGTACTCGGT GCAGCCCGCA 
TTTCCTGCGG TCGATGGTGG GAGCGGGGCC ACTTCATTTG TCAAGGCTGC GCGGTTCCCA 
GATGGCCAAG CTGCCCAAAC CGAAGGACCT GGGCCCGGTG CATACAGCAT TCAATCGGCG 
CAACGGACCT CAGGGCCCTT TATTGGTCGA GCGCCCCGTG TTGCCACAGG GGTTGGTGCC 
AACAATAACG ATCTCAGAGA ACAGCCAGGA CCAGGTCACT ACGAGGTCAG CAGTGGAGGG 
CTTGGGAGTA TTGTGGACGG TGGCGTAGCG ATGCGTTTCA CGAGCGAGCG CTTCCCTGGG 
CTCAGCGGTG GCGCAGTGGG AGAGAGTCCA GGTCCGGCGT CGTACAGTCC CGCAGAGGCC 
AGAGGTACGA AGGATGGTGT GTCGTTCCCA CAGGCACCGC GCCATTCGCC GGCAGAGCTG 
AATGATGCTT CATCGCGTCC TGGACCTGGT GATTATGATA TCCCCTCTAC CTGGAACACC 
ACAGCGGGCA ACACCTATTC CTTTGGTGCT GAAGAGCGCA TGAAAGCCCC CACGGTAGAT 
GCCGATGTAC CGGGGCCCGG TAGCTACGAT ACGAACCGTT ACCTGAACGC ATATCAAGCT 
GGGCCCGCAT TCACTCTTAA AAGTAAACCA GGACAGAATG AAAACGGTGG TGACCTTCCT 
GGTCCTGGGA GCTACGATGC AAGTCGCTAC ATAGATGAAA GTAGGGCGGG TCGTGCGTTT 
ACTCTTCGAG GGAAAAGTAC ATTTAACGGC AGCAGTGGCA ATGGTGATGT TCCTGGTCCG 
GGAAGCTACG ACCCAAATCG CTATCTCGAA CAATGCCAAG CCGGCCCGTC GGTCTCATTG 
AGAGGGAAAG CAGCCCCCGT CGGCGGACAT GATGCTGACG TCCCCGGTCC CGGAAGCTAT 
GACGCGAGTA GCTACTTGGA AAGGTGCCAA ATGGGCCCCG CCTTCTCGTT GAAGGGAAAA 
CCAACCTGCG TTGACGAAAA CAGTGCTGGC ATTCCCGGCC CCGGTAGTTA TGATGCGAAC 
CGATACTTGG AGCAGTTTCA GTCAGGACCC GCGTTCACAC TTCGAGGAAA AGGTGCGTGT 
AATGGCAATG ACAGCGAAGC TGACGTTCCA GGTCCGGGGA GCTACGATCC GAATCGTTAC 
CTCGAACAAC ACCACGCAGG GCCGTCGTTT ACCCTAAAGG GCAAGGCCAC GTATAATGGC 
GAGAATGGTA TTGATGCTCC GGGACCTGGC AGTTATGATA TCAATCGCTG TATGGAACAG 
TCTCAGGCAG GTCCTTCATT CACGTTAAGG GGGAAACCAA GCGACACTGG GCAGAAGGGG 
CCTGATATCC CTGGTCCTGG AAGCTATGAT GCCAGTCAGT ACTTGGAGCG GTGTCAAGCG 
GGTCCTGCCT TCACTCTCCG AGGGAGGGAT GCGTTTAACA GTAACAGTGC CGATGGCGAC 
GTTCCTGGGC CGGGAAGCTA CGATGTGAAT CGATATTTGA AGCAGTGTCA GTCGGGGGTT 
TCATTTACTT TGAAGGGAAA ATCAGCGCAA AACGACAACG AAGCTGACGC TCCAGGTCCG 
GGGAGCTACG ATCCGAATCG TTACCTCGAA CAACACCACG CAGGGCCGTC GTTTACCCTA 
AAGGGCAAGG CCACGTATAA TGGCGAGAAT GGTATTGATG CTCCGGGACC TGGCAGTTAC 
GATATCAATC GCTACATGGA ACTGTGTCAG GCGGGCCCAG CCTTTACTTT GAAAGGAAAA 
GCATCCCAAG TTGAAGGTAG CGCCGATGTA CCTGGCCCCG GTAGCTACGA CGTTAGCCGC 
TATACGGAAC AATTTCAGTC GGGCCCTGCC TTCACGCTCA AAGGAAAACA AGTGACCAGC 
GACGGGGTCG ACGCCGACGT CCCCGGTCCC GGAAGCTACA ACGTAAGCAG CTACGCAGAA 
CAGTTTAAAC CGGGACCAGC TTTTACGGTG AAGGGGAAGC CATCGGGCGC TGCCGAGAAC 
AGTGATGGTT TACCTGGGCC CGGAAGTTAC GACACGGATC GATACATGCA GCAATGCCAT 
TCAGGTCCCG CCTTCACTCT GAAAGGGAAA CCAACGTACG GCGATGGCCG CCAAACGGAA 
GATGTCCCTG GCCCCGGTGC CTATTGTCCA CGACCTACCG AAACAACTCG TGCAGTTTCC 
TTCGGTCCAT TGCCCACCGT TGATTCGACA GCCGTATTTG AAGTGCGGGT GAACGCAACA 
CCCGGGCCTG CAGCGTACGT TATCCCTTCC ACACTAAACC AAAAGGCCGT ACCGTTTGGG 
GCAGCGGAGC GGGAGGTGCA GTGCAGAGCA GAAGATGTTC CAGGACCGGG GGCATATCTA 
ACAGGTGGCC CAGTCGTCTT AAAACCCGGC CCCGCATTTA CCTTCGGCAG GGGGCCACGC 
TCTATGACGC TGGAATCTGC CCAGGAGGAC GCACCCGGTC CCGGTGAATA TCACCACCCC 
GTTGACGCAA GACCCACCGT ACTTGCCTTC ACATTCGCTT CTGCCGATCG CTTCGCTGAG 
TTTGAAGCAG GCAATGGCGT GGGTCCCGGT GAATATTACC ACCCGTACCC TACCGAGACC 
CACGGTGGTC CGGCCTTCAG ATTCCCCAAA TCTATGCGTG CTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

The genome of the African trypanosome Trypanosoma brucei.
Science (New York, N.Y.)309(5733)416-22(2005 Jul)
Berriman M,Ghedin E,Hertz-Fowler C,Blandin G,Renauld H,Bartholomeu DC,Lennard NJ,Caler E,Hamlin NE,Haas B,Böhme U,Hannick L,Aslett MA,Shallom J,Marcello L,Hou L,Wickstead B,Alsmark UC,Arrowsmith C,Atkin RJ,Barron AJ,Bringaud F,Brooks K,Carrington M,Cherevach I,Chillingworth TJ,Churcher C,Clark LN,Corton CH,Cronin A,Davies RM,Doggett J,Djikeng A,Feldblyum T,Field MC,Fraser A,Goodhead I,Hance Z,Harper D,Harris BR,Hauser H,Hostetler J,Ivens A,Jagels K,Johnson D,Johnson J,Jones K,Kerhornou AX,Koo H,Larke N,Landfear S,Larkin C,Leech V,Line A,Lord A,Macleod A,Mooney PJ,Moule S,Martin DM,Morgan GW,Mungall K,Norbertczak H,Ormond D,Pai G,Peacock CS,Peterson J,Quail MA,Rabbinowitsch E,Rajandream MA,Reitter C,Salzberg SL,Sanders M,Schobel S,Sharp S,Simmonds M,Simpson AJ,Tallon L,Turner CM,Tait A,Tivey AR,Van Aken S,Walker D,Wanless D,Wang S,White B,White O,Whitehead S,Woodward J,Wortman J,Adams MD,Embley TM,Gull K,Ullu E,Barry JD,Fairlamb AH,Opperdoes F,Barrell BG,Donelson JE,Hall N,Fraser CM,Melville SE,El-Sayed NM

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Tb927.7.7200 cDNA ORF clone, Trypanosoma brucei brucei TREU927

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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