HADH cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	HADH
Entrez Gene ID	397604
Full Name	hydroxyacyl-CoA dehydrogenase
Synonyms	HAD,HADHSC
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214331.1	NP_999496.1	hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

Pig heart short chain L-3-hydroxyacyl-CoA dehydrogenase revisited: sequence analysis and crystal structure determination.
Barycki JJ, O'Brien LK, Birktoft JJ, Strauss AW, Banaszak LJ
Protein science : a publication of the Protein Society8(10)2010-8(1999 Oct)

Identity of heart and liver L-3-hydroxyacyl coenzyme A dehydrogenase.
He XY, Zhang G, Blecha F, Yang SY
Biochimica et biophysica acta1437(2)119-23(1999 Feb)

Molecular cloning, expression in Escherichia coli, and characterization of a novel L-3-hydroxyacyl coenzyme A dehydrogenase from pig liver.
He XY, Yang SY
Biochimica et biophysica acta1392(1)119-26(1998 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HADH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HADH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe30517	NM_214331.1 Latest version!	Sus scrofa hydroxyacyl-CoA dehydrogenase (HADH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HADH ( NM_214331.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999496.1 Sus scrofa hydroxyacyl-CoA dehydrogenase (HADH), mRNA.

Price & Availability↑

CloneID	OSe30517
Clone ID Related Accession (Same CDS sequence)	NM_214331.1
Accession Version	NM_214331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573660800000
Organism	Sus scrofa(pig)
Product	hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF027652.1. ##Evidence-Data-START## Transcript exon combination :: AF027652.1, AK230471.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01048300, SAMN01048373 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTTTCG CAACCAGGCA GCTCGTGCGC TCCTTGTCCT CCTCTTCCAC CGCCGCGGCC 
TCGGCGAAGA AAATCCTCGT CAAGCACGTG ACGGTCATCG GCGGCGGGCT GATGGGCGCA 
GGCATTGCCC AGGTTGCTGC AGCAACTGGC CACACGGTAG TGTTGGTAGA TCAAACAGAG 
GACATCCTAG CAAAATCTAA AAAGGGAATT GAGGAAAGCC TTCGGAAAGT GGCCAAGAAG 
AAGTTTGCGG AAAACCCCAA GGCTGGCGAT GAATTCGTGG AGAAGACCCT GAGCAGCATA 
TCCACCAGCA CGGACGCAGC ATCCGTGGTC CACAGCACAG ACCTGGTGGT GGAGGCCATC 
GTGGAGAACC TGAAGGTGAA GAGCGAGCTC TTCAAGAGGC TGGACAAGTT TGCTGCTGAG 
CACACAATCT TTGCCAGCAA CACTTCCTCT TTGCAGATCA CAAGCCTAGC CAATGCCACC 
ACCAGACAGG ACCGATTTGC CGGGCTCCAT TTCTTCAACC CAGTGCCCTT GATGAAACTT 
GTGGAAGTCG TCAAAACACC AATGACCAGC CAGAAAACAT TGGAATCTCT GGTTGACTTC 
AGCAAAACCC TGGGAAAACA CCCTGTTTCT TGCAAGGACA CTCCTGGTTT TATCGTGAAC 
CGTCTCCTGG TGCCGTACCT CATAGAAGCA GTCAGGCTGT ACGAACGAGG TGACGCATCC 
AAGGAAGACA TTGATACTGC TATGAAGCTG GGTGCTGGGT ACCCCATGGG CCCATTTGAA 
CTTCTCGATT ATGTTGGACT GGATACTACC AAGTTCATCA TAGATGGGTG GCATGAAATG 
GATTCACAGA ACCCCCTGTT TCAGCCCAGC CCAGCCATGA ATAAGCTGGT AGCGGAGAAT 
AAGTTTGGCA AGAAGACTGG AGAAGGATTT TACAAATACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999496.1
CDS	107..1051
Translation	MAFATRQLVRSLSSSSTAAASAKKILVKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGIEESLRKVAKKKFAENPKAGDEFVEKTLSSISTSTDAASVVHSTDLVVEAIVENLKVKSELFKRLDKFAAEHTIFASNTSSLQITSLANATTRQDRFAGLHFFNPVPLMKLVEVVKTPMTSQKTLESLVDFSKTLGKHPVSCKDTPGFIVNRLLVPYLIEAVRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIIDGWHEMDSQNPLFQPSPAMNKLVAENKFGKKTGEGFYKYK

Target ORF information:

RefSeq Version	NM_214331.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa hydroxyacyl-CoA dehydrogenase (HADH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214331.1

ORF Insert Sequence:

ATGGCTTTCG CAACCAGGCA GCTCGTGCGC TCCTTGTCCT CCTCTTCCAC CGCCGCGGCC 
TCGGCGAAGA AAATCCTCGT CAAGCACGTG ACGGTCATCG GCGGCGGGCT GATGGGCGCA 
GGCATTGCCC AGGTTGCTGC AGCAACTGGC CACACGGTAG TGTTGGTAGA TCAAACAGAG 
GACATCCTAG CAAAATCTAA AAAGGGAATT GAGGAAAGCC TTCGGAAAGT GGCCAAGAAG 
AAGTTTGCGG AAAACCCCAA GGCTGGCGAT GAATTCGTGG AGAAGACCCT GAGCAGCATA 
TCCACCAGCA CGGACGCAGC ATCCGTGGTC CACAGCACAG ACCTGGTGGT GGAGGCCATC 
GTGGAGAACC TGAAGGTGAA GAGCGAGCTC TTCAAGAGGC TGGACAAGTT TGCTGCTGAG 
CACACAATCT TTGCCAGCAA CACTTCCTCT TTGCAGATCA CAAGCCTAGC CAATGCCACC 
ACCAGACAGG ACCGATTTGC CGGGCTCCAT TTCTTCAACC CAGTGCCCTT GATGAAACTT 
GTGGAAGTCG TCAAAACACC AATGACCAGC CAGAAAACAT TGGAATCTCT GGTTGACTTC 
AGCAAAACCC TGGGAAAACA CCCTGTTTCT TGCAAGGACA CTCCTGGTTT TATCGTGAAC 
CGTCTCCTGG TGCCGTACCT CATAGAAGCA GTCAGGCTGT ACGAACGAGG TGACGCATCC 
AAGGAAGACA TTGATACTGC TATGAAGCTG GGTGCTGGGT ACCCCATGGG CCCATTTGAA 
CTTCTCGATT ATGTTGGACT GGATACTACC AAGTTCATCA TAGATGGGTG GCATGAAATG 
GATTCACAGA ACCCCCTGTT TCAGCCCAGC CCAGCCATGA ATAAGCTGGT AGCGGAGAAT 
AAGTTTGGCA AGAAGACTGG AGAAGGATTT TACAAATACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Nucleic acids research35(Database issue)D650-3(2007 Jan)
Uenishi H,Eguchi-Ogawa T,Shinkai H,Okumura N,Suzuki K,Toki D,Hamasima N,Awata T

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

Pig heart short chain L-3-hydroxyacyl-CoA dehydrogenase revisited: sequence analysis and crystal structure determination.
Protein science : a publication of the Protein Society8(10)2010-8(1999 Oct)
Barycki JJ,O'Brien LK,Birktoft JJ,Strauss AW,Banaszak LJ

Identity of heart and liver L-3-hydroxyacyl coenzyme A dehydrogenase.
Biochimica et biophysica acta1437(2)119-23(1999 Feb)
He XY,Zhang G,Blecha F,Yang SY

Molecular cloning, expression in Escherichia coli, and characterization of a novel L-3-hydroxyacyl coenzyme A dehydrogenase from pig liver.
Biochimica et biophysica acta1392(1)119-26(1998 May)
He XY,Yang SY

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HADH cDNA ORF clone, Sus scrofa(pig)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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