EGF cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	EGF
Entrez Gene ID	397083
Full Name	epidermal growth factor
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 8 Map Location: 8q2.3-q2.7

mRNA and Protein(s)

mRNA	Protein	Name
NM_214020.2	NP_999185.1	pro-epidermal growth factor precursor
NM_214020.1	NP_999185.1	pro-epidermal growth factor precursor
XM_021100462.1	XP_020956121.1	pro-epidermal growth factor isoform X2
XM_021100461.1	XP_020956120.1	pro-epidermal growth factor isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Association and polymorphism study of seven candidate genes with reproductive traits in three pig breeds in Hungary.
Hunyadi-Bagi Á, Balogh P, Nagy K, Kusza S
Acta biochimica Polonica63(2)359-64(2016)

Epidermal growth factor can optimize a serum-free culture system for bone marrow stem cell proliferation in a miniature pig model.
Wang X, Zheng F, Liu O, Zheng S, Liu Y, Wang Y, Tang Z, Zhong L
In vitro cellular & developmental biology. Animal49(10)815-25(2013 Dec)

Epidermal growth factor stimulates proliferation and migration of porcine trophectoderm cells through protooncogenic protein kinase 1 and extracellular-signal-regulated kinases 1/2 mitogen-activated protein kinase signal transduction cascades during early pregnancy.
Jeong W, Kim J, Bazer FW, Song G
Molecular and cellular endocrinology381(1-2)302-11(2013 Dec)

Progesterone is essential for maintenance of Tace/Adam17 mRNA expression, but not EGF-like factor, in cumulus cells, which enhances the EGF receptor signaling pathway during in vitro maturation of porcine COCs.
Yamashita Y, Kawashima I, Gunji Y, Hishinuma M, Shimada M
The Journal of reproduction and development56(3)315-23(2010 Jun)

Opposing roles of EGF in IFN-alpha-induced epithelial barrier destabilization and tissue repair.
Lechner J, Malloth NA, Jennings P, Heckl D, Pfaller W, Seppi T
American journal of physiology. Cell physiology293(6)C1843-50(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EGF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe00689	NM_214020.2 Latest version!	Sus scrofa epidermal growth factor (EGF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe223945	XM_021100461.1 Latest version!	Sus scrofa epidermal growth factor (EGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe223946	XM_021100462.1 Latest version!	Sus scrofa epidermal growth factor (EGF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OSe00689	NM_214020.1	Sus scrofa epidermal growth factor (EGF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EGF ( NM_214020.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999185.1 Sus scrofa epidermal growth factor (EGF), mRNA.

Price & Availability↑

CloneID	OSe00689
Clone ID Related Accession (Same CDS sequence)	NM_214020.2 , NM_214020.1
Accession Version	NM_214020.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Sus scrofa(pig)
Product	pro-epidermal growth factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF336151.1. On Mar 1, 2018 this sequence version replaced NM_214020.1. ##Evidence-Data-START## Transcript exon combination :: AF336151.1, SRR5275317.55657.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103886111, SAMEA103886112 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCTCT TCCTTATCCT TCTGTTGCCG GTAGTTTTGA AATTTAGTTT TGTCAGCCTC 
TCAGCACCAG CGCGCTGGAA CTGTCCTGAA GGCTCTCCCT CAGGAAACGG GAATGCCACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ACAGTATCTT TAGGATTGAC 
TTGGAAGGAA CTAATCATGA GCAATTGGTG GCAGATGCTG GAATATCAGT GCTCATGGAC 
TTCCATTACA ATGAGGAAAG AATCTATTGG GTCGATCTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAAA GTATGCAATC TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGCACCATT 
ACAGTAACAG ATATGAAAGG AAACAATTCC CGTGTTCTCT TAAGCGCCTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTGATGTTTT GGTCTTCAGT GGTGGCCGGC 
AGCCTTCACA GGGCAGATGT TACAGGTGTG GAAGTGAGAC TTCTGTTGGA GACATCAGAG 
GAAATTGCAG CTGTGTCATT GGATGTGCTT GATAAACGGC TCTTTTGGAT TCAGTATAAC 
AGAGAAGGAG GCAGTTCTCG TATTTGTTCC TGTGATTATG ATGGAGGTTC TGTCCATTTT 
AGCAAACATC TCACACAGCA CAATGTGTTT GCAATGTCCC TTTTTGGTGA TCACATCTTC 
TATTCAACAT GGAAAAAGAA GACAATTTGG GTAGCTAACA AGCACACTGG GAAGGATATG 
GTTAAAATGA ACCTCAATCC AGCATTTGTA CCACCTGGTG GAATTAAAGT AGTGCATCCA 
CTTGTTCAGC CCAAGGCAGA GGGTGATGCT TGGGCCTCTG ATCAGAAACT CTGCAAACTG 
AGGAAGGGTA ACTGCAGAGG CAGCATGTGT GGGCAAGAGC CAAAGTCCCA CGTGTGCACG 
TGTGCCGAGG GATACACTTT AAGCCAAGAT GGAAGGAAGT GTGAAGATGT CAACGAGTGT 
GCCTTCTGGA ATCATGGCTG TACTCTCGGG TGTGAAAACA CTCCTGGATC CTATTATTGC 
ACGTGTCCTG CAGGATTTGT TCTGCTCCCT GATGGGAAAC GGTGTCATCA ATTAATTTCC 
TGCCCAAGCA ATGTGTCTGA GTGTAGCCAT GACTGTGTTC TGACATCAGA TGGTCCCATA 
TGTTTCTGTC CTGAAGACTC AGTACTTGAG GCAGATGGAA AAACATGTAG CGGCTGTTCA 
TCACCTGATA ATGGTGGGTG CAGCCAGCTC TGCCTTCCTC TCAGTCCAGT AACCTGGGAA 
TGTGGCTGCT TTCCTGGGTA TGACCTACAG TTGGACAAAA AGAGCTGTAG AGCTTCAGGA 
CCACCACCGT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGACGGA 
ACAGATTATG AAACTCTGCT CAACCAGCAA ATAGGAATGG TTTTGGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGGTATA CTTTGCCCAT ACAGCCCTGA AATGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTTTT GAGGAAGCAG TCGATGTGCC AGAAGGTCTT 
GCCATAGACT GGATTGGCCG TAAATTCTAT TGGACAGACA GAGGGAGATC TCTCATTGAA 
GGGAGTGATT TAAATGGAAA ATACCGCGAA ATAATCATTA AGGAAGACAT CTCTCAGCCA 
CGAGGAATTG CTGTTCATCC AGTGGCCAAG AGATTATTCT GGACTGATAT GGGGACTAAT 
CCACGAATTG AAAGTTCATC CCTTCAAGGC ATTGGCCGAC AGGTTATAGC CAGCTCGGAT 
CTGGTCTGGC CCAGCGGAAT AACAATTGAT TACTTAACTG ACAAGTTGTA TTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGTCCAAT CTGGATGGTT CGAGACGCCA GAGACTTGCC 
CAGAACGATG TAGGTCACCC ATTTGCGGTA GCTGTGTTTG AGGATCACGT GTGGTTCTCT 
GACTGGACTA TGCCATCGGT AATAAGGGTG AACAAGAGGA CTGGCAAAAA TAGGGTACGT 
CTCCGAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTAG TTCATCCATT GGCAAAACCA 
GGAACAAATC CCTGCTTACA CCAAAATGGA GGCTGTGAAC ATATCTGCAA AGAGAGTTTT 
GGAACTGCTC AGTGTTTGTG TCATGAAGGT TTTCTGAAAG CCCCAGATGG AAAAATGTGT 
CTGGCTCTGA ATGGCCAAGA GATATTGGCA GGTCGTGGGA AAGATCTGAG TGATGGAGTA 
ATGCCAGTGG ACACCTTACC CAGAAGCAGA GAGCTTGAAG ATAACCTTAC AGAATCTCAA 
CACATTCTAG TGGCTGAAAT CATGGTTTCA GATGACGAGG ACTGCGGCGC TGCGGGATGC 
AGTGCGCAGG CGCGGTGTGT TACAGAGGGA GAGGACGCCA CGTGTCAGTG TTTGAAAGGA 
TTTGCTGGAG ATGGAAACCT GTGTTCTGAT ATAGATGAGT GTGAGCTGGG CACCTCGGTG 
TGCCCTCCTA CCTCCTCCGA GTGCATCAAT ACCGAAGGTG GTCACGTCTG CCGCTGCTCG 
GAAGGCTACC AGGGAGATGG GATCCACTGT CTCGATATTG ATGAGTGCCA ACTAGGTGTG 
CACACCTGTG GGGAAAATGC CACCTGTACA AATACGGAGG GAAACTACAC CTGCACATGT 
GCTGGCCGCC CCTCTGAACC CGGACGGATT TGCCCTGACC CTACTCCACC CTCTCACCTC 
GGGGAGGATG GCCGCTATTC TGTGAGAAAT AGTTACTCTG AATGCCCGCC GTCCCACGAC 
GGGTACTGCC TCCACGGTGG TGTGTGTATG TATATTGAAG CCGTCGACAG CTATGCCTGC 
AACTGTGTTT TTGGCTACGT TGGCGAGCGA TGTCAGCACA GAGACTTGAA ATGGTGGGAG 
CTGCGCCACG CTGGCCTCGG GCGACAGTGG AACGTCACGG TGGTGGCCGT CTGCGTGGTG 
GTGCTGGTCC TGCTGCTGCT CCTGGGGCTG TGGGGGGCTC ACTACTACAG GACTCAGAAG 
CTGCTATCAA AAAATCCAAA GAATCCTTAT GAAGAGTCAG GCAGAGATGT GAGTGGTATC 
AGGCCTGCAG ACGGCGAGGC TGGGATGTCC TCTTGCCCCC AACCTTGGTT TGTGGTGATA 
AAGGAACATC AAAATCTCAG GAATGGGAGT CAACCTGGAG CCCCCAAGGA CGGCCTGGGA 
GCAGATGTTG GCCAGTTTTC TTCCCTGGAG CCTGGGTCAT TGCAGCCGAC ATCATGGAGG 
AAGGAACCCC AGATGTATAT GGACACAGAA CAAGGATGCT GTATTCCATC CTCCAGTGAT 
AAAGGCTCTG GTCCCCAGGG AATAGGGTAT AGCTTTCATC TCCCCTCCTA TGGGGCACGG 
TCCATTGCTG TCGGGGTTGA AAAATCCCAT TCTCTCCTGT CAGCTAACCC ATTACGGCAA 
CAAAGGGCTC CAGATCCACC ACACCAAATG GAGCTGACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999185.1
CDS	437..4081
Translation	MLLFLILLLPVVLKFSFVSLSAPARWNCPEGSPSGNGNATCVGPAPFLIFSHGNSIFRIDLEGTNHEQLVADAGISVLMDFHYNEERIYWVDLERQLLQRVFLNGTRQEKVCNLEKNVSGMAINWINEELIWSNQQEGTITVTDMKGNNSRVLLSALKYPANVAVDPVERLMFWSSVVAGSLHRADVTGVEVRLLLETSEEIAAVSLDVLDKRLFWIQYNREGGSSRICSCDYDGGSVHFSKHLTQHNVFAMSLFGDHIFYSTWKKKTIWVANKHTGKDMVKMNLNPAFVPPGGIKVVHPLVQPKAEGDAWASDQKLCKLRKGNCRGSMCGQEPKSHVCTCAEGYTLSQDGRKCEDVNECAFWNHGCTLGCENTPGSYYCTCPAGFVLLPDGKRCHQLISCPSNVSECSHDCVLTSDGPICFCPEDSVLEADGKTCSGCSSPDNGGCSQLCLPLSPVTWECGCFPGYDLQLDKKSCRASGPPPFLLFANSQDIRHMHFDGTDYETLLNQQIGMVLALDHDPVENKVYFAHTALKWIERANMDGSQRERLFEEAVDVPEGLAIDWIGRKFYWTDRGRSLIEGSDLNGKYREIIIKEDISQPRGIAVHPVAKRLFWTDMGTNPRIESSSLQGIGRQVIASSDLVWPSGITIDYLTDKLYWCDAKQSVIEMSNLDGSRRQRLAQNDVGHPFAVAVFEDHVWFSDWTMPSVIRVNKRTGKNRVRLRGSMLKPSSLVVVHPLAKPGTNPCLHQNGGCEHICKESFGTAQCLCHEGFLKAPDGKMCLALNGQEILAGRGKDLSDGVMPVDTLPRSRELEDNLTESQHILVAEIMVSDDEDCGAAGCSAQARCVTEGEDATCQCLKGFAGDGNLCSDIDECELGTSVCPPTSSECINTEGGHVCRCSEGYQGDGIHCLDIDECQLGVHTCGENATCTNTEGNYTCTCAGRPSEPGRICPDPTPPSHLGEDGRYSVRNSYSECPPSHDGYCLHGGVCMYIEAVDSYACNCVFGYVGERCQHRDLKWWELRHAGLGRQWNVTVVAVCVVVLVLLLLLGLWGAHYYRTQKLLSKNPKNPYEESGRDVSGIRPADGEAGMSSCPQPWFVVIKEHQNLRNGSQPGAPKDGLGADVGQFSSLEPGSLQPTSWRKEPQMYMDTEQGCCIPSSSDKGSGPQGIGYSFHLPSYGARSIAVGVEKSHSLLSANPLRQQRAPDPPHQMELTQ

Target ORF information:

RefSeq Version	NM_214020.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa epidermal growth factor (EGF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214020.2

ORF Insert Sequence:

ATGCTGCTCT TCCTTATCCT TCTGTTGCCG GTAGTTTTGA AATTTAGTTT TGTCAGCCTC 
TCAGCACCAG CGCGCTGGAA CTGTCCTGAA GGCTCTCCCT CAGGAAACGG GAATGCCACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ACAGTATCTT TAGGATTGAC 
TTGGAAGGAA CTAATCATGA GCAATTGGTG GCAGATGCTG GAATATCAGT GCTCATGGAC 
TTCCATTACA ATGAGGAAAG AATCTATTGG GTCGATCTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAAA GTATGCAATC TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGCACCATT 
ACAGTAACAG ATATGAAAGG AAACAATTCC CGTGTTCTCT TAAGCGCCTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTGATGTTTT GGTCTTCAGT GGTGGCCGGC 
AGCCTTCACA GGGCAGATGT TACAGGTGTG GAAGTGAGAC TTCTGTTGGA GACATCAGAG 
GAAATTGCAG CTGTGTCATT GGATGTGCTT GATAAACGGC TCTTTTGGAT TCAGTATAAC 
AGAGAAGGAG GCAGTTCTCG TATTTGTTCC TGTGATTATG ATGGAGGTTC TGTCCATTTT 
AGCAAACATC TCACACAGCA CAATGTGTTT GCAATGTCCC TTTTTGGTGA TCACATCTTC 
TATTCAACAT GGAAAAAGAA GACAATTTGG GTAGCTAACA AGCACACTGG GAAGGATATG 
GTTAAAATGA ACCTCAATCC AGCATTTGTA CCACCTGGTG GAATTAAAGT AGTGCATCCA 
CTTGTTCAGC CCAAGGCAGA GGGTGATGCT TGGGCCTCTG ATCAGAAACT CTGCAAACTG 
AGGAAGGGTA ACTGCAGAGG CAGCATGTGT GGGCAAGAGC CAAAGTCCCA CGTGTGCACG 
TGTGCCGAGG GATACACTTT AAGCCAAGAT GGAAGGAAGT GTGAAGATGT CAACGAGTGT 
GCCTTCTGGA ATCATGGCTG TACTCTCGGG TGTGAAAACA CTCCTGGATC CTATTATTGC 
ACGTGTCCTG CAGGATTTGT TCTGCTCCCT GATGGGAAAC GGTGTCATCA ATTAATTTCC 
TGCCCAAGCA ATGTGTCTGA GTGTAGCCAT GACTGTGTTC TGACATCAGA TGGTCCCATA 
TGTTTCTGTC CTGAAGACTC AGTACTTGAG GCAGATGGAA AAACATGTAG CGGCTGTTCA 
TCACCTGATA ATGGTGGGTG CAGCCAGCTC TGCCTTCCTC TCAGTCCAGT AACCTGGGAA 
TGTGGCTGCT TTCCTGGGTA TGACCTACAG TTGGACAAAA AGAGCTGTAG AGCTTCAGGA 
CCACCACCGT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGACGGA 
ACAGATTATG AAACTCTGCT CAACCAGCAA ATAGGAATGG TTTTGGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGGTATA CTTTGCCCAT ACAGCCCTGA AATGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTTTT GAGGAAGCAG TCGATGTGCC AGAAGGTCTT 
GCCATAGACT GGATTGGCCG TAAATTCTAT TGGACAGACA GAGGGAGATC TCTCATTGAA 
GGGAGTGATT TAAATGGAAA ATACCGCGAA ATAATCATTA AGGAAGACAT CTCTCAGCCA 
CGAGGAATTG CTGTTCATCC AGTGGCCAAG AGATTATTCT GGACTGATAT GGGGACTAAT 
CCACGAATTG AAAGTTCATC CCTTCAAGGC ATTGGCCGAC AGGTTATAGC CAGCTCGGAT 
CTGGTCTGGC CCAGCGGAAT AACAATTGAT TACTTAACTG ACAAGTTGTA TTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGTCCAAT CTGGATGGTT CGAGACGCCA GAGACTTGCC 
CAGAACGATG TAGGTCACCC ATTTGCGGTA GCTGTGTTTG AGGATCACGT GTGGTTCTCT 
GACTGGACTA TGCCATCGGT AATAAGGGTG AACAAGAGGA CTGGCAAAAA TAGGGTACGT 
CTCCGAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTAG TTCATCCATT GGCAAAACCA 
GGAACAAATC CCTGCTTACA CCAAAATGGA GGCTGTGAAC ATATCTGCAA AGAGAGTTTT 
GGAACTGCTC AGTGTTTGTG TCATGAAGGT TTTCTGAAAG CCCCAGATGG AAAAATGTGT 
CTGGCTCTGA ATGGCCAAGA GATATTGGCA GGTCGTGGGA AAGATCTGAG TGATGGAGTA 
ATGCCAGTGG ACACCTTACC CAGAAGCAGA GAGCTTGAAG ATAACCTTAC AGAATCTCAA 
CACATTCTAG TGGCTGAAAT CATGGTTTCA GATGACGAGG ACTGCGGCGC TGCGGGATGC 
AGTGCGCAGG CGCGGTGTGT TACAGAGGGA GAGGACGCCA CGTGTCAGTG TTTGAAAGGA 
TTTGCTGGAG ATGGAAACCT GTGTTCTGAT ATAGATGAGT GTGAGCTGGG CACCTCGGTG 
TGCCCTCCTA CCTCCTCCGA GTGCATCAAT ACCGAAGGTG GTCACGTCTG CCGCTGCTCG 
GAAGGCTACC AGGGAGATGG GATCCACTGT CTCGATATTG ATGAGTGCCA ACTAGGTGTG 
CACACCTGTG GGGAAAATGC CACCTGTACA AATACGGAGG GAAACTACAC CTGCACATGT 
GCTGGCCGCC CCTCTGAACC CGGACGGATT TGCCCTGACC CTACTCCACC CTCTCACCTC 
GGGGAGGATG GCCGCTATTC TGTGAGAAAT AGTTACTCTG AATGCCCGCC GTCCCACGAC 
GGGTACTGCC TCCACGGTGG TGTGTGTATG TATATTGAAG CCGTCGACAG CTATGCCTGC 
AACTGTGTTT TTGGCTACGT TGGCGAGCGA TGTCAGCACA GAGACTTGAA ATGGTGGGAG 
CTGCGCCACG CTGGCCTCGG GCGACAGTGG AACGTCACGG TGGTGGCCGT CTGCGTGGTG 
GTGCTGGTCC TGCTGCTGCT CCTGGGGCTG TGGGGGGCTC ACTACTACAG GACTCAGAAG 
CTGCTATCAA AAAATCCAAA GAATCCTTAT GAAGAGTCAG GCAGAGATGT GAGTGGTATC 
AGGCCTGCAG ACGGCGAGGC TGGGATGTCC TCTTGCCCCC AACCTTGGTT TGTGGTGATA 
AAGGAACATC AAAATCTCAG GAATGGGAGT CAACCTGGAG CCCCCAAGGA CGGCCTGGGA 
GCAGATGTTG GCCAGTTTTC TTCCCTGGAG CCTGGGTCAT TGCAGCCGAC ATCATGGAGG 
AAGGAACCCC AGATGTATAT GGACACAGAA CAAGGATGCT GTATTCCATC CTCCAGTGAT 
AAAGGCTCTG GTCCCCAGGG AATAGGGTAT AGCTTTCATC TCCCCTCCTA TGGGGCACGG 
TCCATTGCTG TCGGGGTTGA AAAATCCCAT TCTCTCCTGT CAGCTAACCC ATTACGGCAA 
CAAAGGGCTC CAGATCCACC ACACCAAATG GAGCTGACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( NM_214020.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999185.1 Sus scrofa epidermal growth factor (EGF), mRNA.

Price & Availability↑

CloneID	OSe00689
Clone ID Related Accession (Same CDS sequence)	NM_214020.2 , NM_214020.1
Accession Version	NM_214020.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509120000000
Organism	Sus scrofa(pig)
Product	pro-epidermal growth factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF336151.1. ##Evidence-Data-START## Transcript exon combination :: AF336151.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTGCTCT TCCTTATCCT TCTGTTGCCG GTAGTTTTGA AATTTAGTTT TGTCAGCCTC 
TCAGCACCAG CGCGCTGGAA CTGTCCTGAA GGCTCTCCCT CAGGAAACGG GAATGCCACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ACAGTATCTT TAGGATTGAC 
TTGGAAGGAA CTAATCATGA GCAATTGGTG GCAGATGCTG GAATATCAGT GCTCATGGAC 
TTCCATTACA ATGAGGAAAG AATCTATTGG GTCGATCTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAAA GTATGCAATC TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGCACCATT 
ACAGTAACAG ATATGAAAGG AAACAATTCC CGTGTTCTCT TAAGCGCCTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTGATGTTTT GGTCTTCAGT GGTGGCCGGC 
AGCCTTCACA GGGCAGATGT TACAGGTGTG GAAGTGAGAC TTCTGTTGGA GACATCAGAG 
GAAATTGCAG CTGTGTCATT GGATGTGCTT GATAAACGGC TCTTTTGGAT TCAGTATAAC 
AGAGAAGGAG GCAGTTCTCG TATTTGTTCC TGTGATTATG ATGGAGGTTC TGTCCATTTT 
AGCAAACATC TCACACAGCA CAATGTGTTT GCAATGTCCC TTTTTGGTGA TCACATCTTC 
TATTCAACAT GGAAAAAGAA GACAATTTGG GTAGCTAACA AGCACACTGG GAAGGATATG 
GTTAAAATGA ACCTCAATCC AGCATTTGTA CCACCTGGTG GAATTAAAGT AGTGCATCCA 
CTTGTTCAGC CCAAGGCAGA GGGTGATGCT TGGGCCTCTG ATCAGAAACT CTGCAAACTG 
AGGAAGGGTA ACTGCAGAGG CAGCATGTGT GGGCAAGAGC CAAAGTCCCA CGTGTGCACG 
TGTGCCGAGG GATACACTTT AAGCCAAGAT GGAAGGAAGT GTGAAGATGT CAACGAGTGT 
GCCTTCTGGA ATCATGGCTG TACTCTCGGG TGTGAAAACA CTCCTGGATC CTATTATTGC 
ACGTGTCCTG CAGGATTTGT TCTGCTCCCT GATGGGAAAC GGTGTCATCA ATTAATTTCC 
TGCCCAAGCA ATGTGTCTGA GTGTAGCCAT GACTGTGTTC TGACATCAGA TGGTCCCATA 
TGTTTCTGTC CTGAAGACTC AGTACTTGAG GCAGATGGAA AAACATGTAG CGGCTGTTCA 
TCACCTGATA ATGGTGGGTG CAGCCAGCTC TGCCTTCCTC TCAGTCCAGT AACCTGGGAA 
TGTGGCTGCT TTCCTGGGTA TGACCTACAG TTGGACAAAA AGAGCTGTAG AGCTTCAGGA 
CCACCACCGT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGACGGA 
ACAGATTATG AAACTCTGCT CAACCAGCAA ATAGGAATGG TTTTGGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGGTATA CTTTGCCCAT ACAGCCCTGA AATGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTTTT GAGGAAGCAG TCGATGTGCC AGAAGGTCTT 
GCCATAGACT GGATTGGCCG TAAATTCTAT TGGACAGACA GAGGGAGATC TCTCATTGAA 
GGGAGTGATT TAAATGGAAA ATACCGCGAA ATAATCATTA AGGAAGACAT CTCTCAGCCA 
CGAGGAATTG CTGTTCATCC AGTGGCCAAG AGATTATTCT GGACTGATAT GGGGACTAAT 
CCACGAATTG AAAGTTCATC CCTTCAAGGC ATTGGCCGAC AGGTTATAGC CAGCTCGGAT 
CTGGTCTGGC CCAGCGGAAT AACAATTGAT TACTTAACTG ACAAGTTGTA TTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGTCCAAT CTGGATGGTT CGAGACGCCA GAGACTTGCC 
CAGAACGATG TAGGTCACCC ATTTGCGGTA GCTGTGTTTG AGGATCACGT GTGGTTCTCT 
GACTGGACTA TGCCATCGGT AATAAGGGTG AACAAGAGGA CTGGCAAAAA TAGGGTACGT 
CTCCGAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTAG TTCATCCATT GGCAAAACCA 
GGAACAAATC CCTGCTTACA CCAAAATGGA GGCTGTGAAC ATATCTGCAA AGAGAGTTTT 
GGAACTGCTC AGTGTTTGTG TCATGAAGGT TTTCTGAAAG CCCCAGATGG AAAAATGTGT 
CTGGCTCTGA ATGGCCAAGA GATATTGGCA GGTCGTGGGA AAGATCTGAG TGATGGAGTA 
ATGCCAGTGG ACACCTTACC CAGAAGCAGA GAGCTTGAAG ATAACCTTAC AGAATCTCAA 
CACATTCTAG TGGCTGAAAT CATGGTTTCA GATGACGAGG ACTGCGGCGC TGCGGGATGC 
AGTGCGCAGG CGCGGTGTGT TACAGAGGGA GAGGACGCCA CGTGTCAGTG TTTGAAAGGA 
TTTGCTGGAG ATGGAAACCT GTGTTCTGAT ATAGATGAGT GTGAGCTGGG CACCTCGGTG 
TGCCCTCCTA CCTCCTCCGA GTGCATCAAT ACCGAAGGTG GTCACGTCTG CCGCTGCTCG 
GAAGGCTACC AGGGAGATGG GATCCACTGT CTCGATATTG ATGAGTGCCA ACTAGGTGTG 
CACACCTGTG GGGAAAATGC CACCTGTACA AATACGGAGG GAAACTACAC CTGCACATGT 
GCTGGCCGCC CCTCTGAACC CGGACGGATT TGCCCTGACC CTACTCCACC CTCTCACCTC 
GGGGAGGATG GCCGCTATTC TGTGAGAAAT AGTTACTCTG AATGCCCGCC GTCCCACGAC 
GGGTACTGCC TCCACGGTGG TGTGTGTATG TATATTGAAG CCGTCGACAG CTATGCCTGC 
AACTGTGTTT TTGGCTACGT TGGCGAGCGA TGTCAGCACA GAGACTTGAA ATGGTGGGAG 
CTGCGCCACG CTGGCCTCGG GCGACAGTGG AACGTCACGG TGGTGGCCGT CTGCGTGGTG 
GTGCTGGTCC TGCTGCTGCT CCTGGGGCTG TGGGGGGCTC ACTACTACAG GACTCAGAAG 
CTGCTATCAA AAAATCCAAA GAATCCTTAT GAAGAGTCAG GCAGAGATGT GAGTGGTATC 
AGGCCTGCAG ACGGCGAGGC TGGGATGTCC TCTTGCCCCC AACCTTGGTT TGTGGTGATA 
AAGGAACATC AAAATCTCAG GAATGGGAGT CAACCTGGAG CCCCCAAGGA CGGCCTGGGA 
GCAGATGTTG GCCAGTTTTC TTCCCTGGAG CCTGGGTCAT TGCAGCCGAC ATCATGGAGG 
AAGGAACCCC AGATGTATAT GGACACAGAA CAAGGATGCT GTATTCCATC CTCCAGTGAT 
AAAGGCTCTG GTCCCCAGGG AATAGGGTAT AGCTTTCATC TCCCCTCCTA TGGGGCACGG 
TCCATTGCTG TCGGGGTTGA AAAATCCCAT TCTCTCCTGT CAGCTAACCC ATTACGGCAA 
CAAAGGGCTC CAGATCCACC ACACCAAATG GAGCTGACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999185.1
CDS	439..4083
Translation	MLLFLILLLPVVLKFSFVSLSAPARWNCPEGSPSGNGNATCVGPAPFLIFSHGNSIFRIDLEGTNHEQLVADAGISVLMDFHYNEERIYWVDLERQLLQRVFLNGTRQEKVCNLEKNVSGMAINWINEELIWSNQQEGTITVTDMKGNNSRVLLSALKYPANVAVDPVERLMFWSSVVAGSLHRADVTGVEVRLLLETSEEIAAVSLDVLDKRLFWIQYNREGGSSRICSCDYDGGSVHFSKHLTQHNVFAMSLFGDHIFYSTWKKKTIWVANKHTGKDMVKMNLNPAFVPPGGIKVVHPLVQPKAEGDAWASDQKLCKLRKGNCRGSMCGQEPKSHVCTCAEGYTLSQDGRKCEDVNECAFWNHGCTLGCENTPGSYYCTCPAGFVLLPDGKRCHQLISCPSNVSECSHDCVLTSDGPICFCPEDSVLEADGKTCSGCSSPDNGGCSQLCLPLSPVTWECGCFPGYDLQLDKKSCRASGPPPFLLFANSQDIRHMHFDGTDYETLLNQQIGMVLALDHDPVENKVYFAHTALKWIERANMDGSQRERLFEEAVDVPEGLAIDWIGRKFYWTDRGRSLIEGSDLNGKYREIIIKEDISQPRGIAVHPVAKRLFWTDMGTNPRIESSSLQGIGRQVIASSDLVWPSGITIDYLTDKLYWCDAKQSVIEMSNLDGSRRQRLAQNDVGHPFAVAVFEDHVWFSDWTMPSVIRVNKRTGKNRVRLRGSMLKPSSLVVVHPLAKPGTNPCLHQNGGCEHICKESFGTAQCLCHEGFLKAPDGKMCLALNGQEILAGRGKDLSDGVMPVDTLPRSRELEDNLTESQHILVAEIMVSDDEDCGAAGCSAQARCVTEGEDATCQCLKGFAGDGNLCSDIDECELGTSVCPPTSSECINTEGGHVCRCSEGYQGDGIHCLDIDECQLGVHTCGENATCTNTEGNYTCTCAGRPSEPGRICPDPTPPSHLGEDGRYSVRNSYSECPPSHDGYCLHGGVCMYIEAVDSYACNCVFGYVGERCQHRDLKWWELRHAGLGRQWNVTVVAVCVVVLVLLLLLGLWGAHYYRTQKLLSKNPKNPYEESGRDVSGIRPADGEAGMSSCPQPWFVVIKEHQNLRNGSQPGAPKDGLGADVGQFSSLEPGSLQPTSWRKEPQMYMDTEQGCCIPSSSDKGSGPQGIGYSFHLPSYGARSIAVGVEKSHSLLSANPLRQQRAPDPPHQMELTQ

Target ORF information:

RefSeq Version	NM_214020.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa epidermal growth factor (EGF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214020.1

ORF Insert Sequence:

ATGCTGCTCT TCCTTATCCT TCTGTTGCCG GTAGTTTTGA AATTTAGTTT TGTCAGCCTC 
TCAGCACCAG CGCGCTGGAA CTGTCCTGAA GGCTCTCCCT CAGGAAACGG GAATGCCACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ACAGTATCTT TAGGATTGAC 
TTGGAAGGAA CTAATCATGA GCAATTGGTG GCAGATGCTG GAATATCAGT GCTCATGGAC 
TTCCATTACA ATGAGGAAAG AATCTATTGG GTCGATCTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAAA GTATGCAATC TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGCACCATT 
ACAGTAACAG ATATGAAAGG AAACAATTCC CGTGTTCTCT TAAGCGCCTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTGATGTTTT GGTCTTCAGT GGTGGCCGGC 
AGCCTTCACA GGGCAGATGT TACAGGTGTG GAAGTGAGAC TTCTGTTGGA GACATCAGAG 
GAAATTGCAG CTGTGTCATT GGATGTGCTT GATAAACGGC TCTTTTGGAT TCAGTATAAC 
AGAGAAGGAG GCAGTTCTCG TATTTGTTCC TGTGATTATG ATGGAGGTTC TGTCCATTTT 
AGCAAACATC TCACACAGCA CAATGTGTTT GCAATGTCCC TTTTTGGTGA TCACATCTTC 
TATTCAACAT GGAAAAAGAA GACAATTTGG GTAGCTAACA AGCACACTGG GAAGGATATG 
GTTAAAATGA ACCTCAATCC AGCATTTGTA CCACCTGGTG GAATTAAAGT AGTGCATCCA 
CTTGTTCAGC CCAAGGCAGA GGGTGATGCT TGGGCCTCTG ATCAGAAACT CTGCAAACTG 
AGGAAGGGTA ACTGCAGAGG CAGCATGTGT GGGCAAGAGC CAAAGTCCCA CGTGTGCACG 
TGTGCCGAGG GATACACTTT AAGCCAAGAT GGAAGGAAGT GTGAAGATGT CAACGAGTGT 
GCCTTCTGGA ATCATGGCTG TACTCTCGGG TGTGAAAACA CTCCTGGATC CTATTATTGC 
ACGTGTCCTG CAGGATTTGT TCTGCTCCCT GATGGGAAAC GGTGTCATCA ATTAATTTCC 
TGCCCAAGCA ATGTGTCTGA GTGTAGCCAT GACTGTGTTC TGACATCAGA TGGTCCCATA 
TGTTTCTGTC CTGAAGACTC AGTACTTGAG GCAGATGGAA AAACATGTAG CGGCTGTTCA 
TCACCTGATA ATGGTGGGTG CAGCCAGCTC TGCCTTCCTC TCAGTCCAGT AACCTGGGAA 
TGTGGCTGCT TTCCTGGGTA TGACCTACAG TTGGACAAAA AGAGCTGTAG AGCTTCAGGA 
CCACCACCGT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGACGGA 
ACAGATTATG AAACTCTGCT CAACCAGCAA ATAGGAATGG TTTTGGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGGTATA CTTTGCCCAT ACAGCCCTGA AATGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTTTT GAGGAAGCAG TCGATGTGCC AGAAGGTCTT 
GCCATAGACT GGATTGGCCG TAAATTCTAT TGGACAGACA GAGGGAGATC TCTCATTGAA 
GGGAGTGATT TAAATGGAAA ATACCGCGAA ATAATCATTA AGGAAGACAT CTCTCAGCCA 
CGAGGAATTG CTGTTCATCC AGTGGCCAAG AGATTATTCT GGACTGATAT GGGGACTAAT 
CCACGAATTG AAAGTTCATC CCTTCAAGGC ATTGGCCGAC AGGTTATAGC CAGCTCGGAT 
CTGGTCTGGC CCAGCGGAAT AACAATTGAT TACTTAACTG ACAAGTTGTA TTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGTCCAAT CTGGATGGTT CGAGACGCCA GAGACTTGCC 
CAGAACGATG TAGGTCACCC ATTTGCGGTA GCTGTGTTTG AGGATCACGT GTGGTTCTCT 
GACTGGACTA TGCCATCGGT AATAAGGGTG AACAAGAGGA CTGGCAAAAA TAGGGTACGT 
CTCCGAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTAG TTCATCCATT GGCAAAACCA 
GGAACAAATC CCTGCTTACA CCAAAATGGA GGCTGTGAAC ATATCTGCAA AGAGAGTTTT 
GGAACTGCTC AGTGTTTGTG TCATGAAGGT TTTCTGAAAG CCCCAGATGG AAAAATGTGT 
CTGGCTCTGA ATGGCCAAGA GATATTGGCA GGTCGTGGGA AAGATCTGAG TGATGGAGTA 
ATGCCAGTGG ACACCTTACC CAGAAGCAGA GAGCTTGAAG ATAACCTTAC AGAATCTCAA 
CACATTCTAG TGGCTGAAAT CATGGTTTCA GATGACGAGG ACTGCGGCGC TGCGGGATGC 
AGTGCGCAGG CGCGGTGTGT TACAGAGGGA GAGGACGCCA CGTGTCAGTG TTTGAAAGGA 
TTTGCTGGAG ATGGAAACCT GTGTTCTGAT ATAGATGAGT GTGAGCTGGG CACCTCGGTG 
TGCCCTCCTA CCTCCTCCGA GTGCATCAAT ACCGAAGGTG GTCACGTCTG CCGCTGCTCG 
GAAGGCTACC AGGGAGATGG GATCCACTGT CTCGATATTG ATGAGTGCCA ACTAGGTGTG 
CACACCTGTG GGGAAAATGC CACCTGTACA AATACGGAGG GAAACTACAC CTGCACATGT 
GCTGGCCGCC CCTCTGAACC CGGACGGATT TGCCCTGACC CTACTCCACC CTCTCACCTC 
GGGGAGGATG GCCGCTATTC TGTGAGAAAT AGTTACTCTG AATGCCCGCC GTCCCACGAC 
GGGTACTGCC TCCACGGTGG TGTGTGTATG TATATTGAAG CCGTCGACAG CTATGCCTGC 
AACTGTGTTT TTGGCTACGT TGGCGAGCGA TGTCAGCACA GAGACTTGAA ATGGTGGGAG 
CTGCGCCACG CTGGCCTCGG GCGACAGTGG AACGTCACGG TGGTGGCCGT CTGCGTGGTG 
GTGCTGGTCC TGCTGCTGCT CCTGGGGCTG TGGGGGGCTC ACTACTACAG GACTCAGAAG 
CTGCTATCAA AAAATCCAAA GAATCCTTAT GAAGAGTCAG GCAGAGATGT GAGTGGTATC 
AGGCCTGCAG ACGGCGAGGC TGGGATGTCC TCTTGCCCCC AACCTTGGTT TGTGGTGATA 
AAGGAACATC AAAATCTCAG GAATGGGAGT CAACCTGGAG CCCCCAAGGA CGGCCTGGGA 
GCAGATGTTG GCCAGTTTTC TTCCCTGGAG CCTGGGTCAT TGCAGCCGAC ATCATGGAGG 
AAGGAACCCC AGATGTATAT GGACACAGAA CAAGGATGCT GTATTCCATC CTCCAGTGAT 
AAAGGCTCTG GTCCCCAGGG AATAGGGTAT AGCTTTCATC TCCCCTCCTA TGGGGCACGG 
TCCATTGCTG TCGGGGTTGA AAAATCCCAT TCTCTCCTGT CAGCTAACCC ATTACGGCAA 
CAAAGGGCTC CAGATCCACC ACACCAAATG GAGCTGACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_021100461.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020956120.1 Sus scrofa epidermal growth factor (EGF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe223945
Clone ID Related Accession (Same CDS sequence)	XM_021100461.1
Accession Version	XM_021100461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	pro-epidermal growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCT TCCTTATCCT TCTGTTGCCG GTAGTTTTGA AATTTAGTTT TGTCAGCCTC 
TCAGCACCAG CGCGCTGGAA CTGTCCTGAA GGCTCTCCCT CAGGAAACGG GAATGCCACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ACAGTATCTT TAGGATTGAC 
TTGGAAGGAA CTAATCATGA GCAATTGGTG GCAGATGCTG GAATATCAGT GCTCATGGAC 
TTCCATTACA ATGAGGAAAG AATCTATTGG GTCGATCTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAAA GTATGCAATC TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGCACCATT 
ACAGTAACAG ATATGAAAGG AAACAATTCC CGTGTTCTCT TAAGCGCCTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTGATGTTTT GGTCTTCAGT GGTGGCCGGC 
AGCCTTCACA GGGCAGATGT TACAGGTGTG GAAGTGAGAC TTCTGTTGGA GACATCAGAG 
GAAATTGCAG CTGTGTCATT GGATGTGCTT GATAAACGGC TCTTTTGGAT TCAGTATAAC 
AGAGAAGGAG GCAGTTCTCG TATTTGTTCC TGTGATTATG ATGGAGGTTC TGTCCATTTT 
AGCAAACATC TCACACAGCA CAATGTGTTT GCAATGTCCC TTTTTGGTGA TCACATCTTC 
TATTCAACAT GGAAAAAGAA GACAATTTGG GTAGCTAACA AGCACACTGG GAAGGATATG 
GTTAAAATGA ACCTCAATCC AGCATTTGTA CCACCTGGTG GAATTAAAGT AGTGCATCCA 
CTTGTTCAGC CCAAGGCAGA GGGTGATGCT TGGGCCTCTG ATCAGAAACT CTGCAAACTG 
AGGAAGGGTA ACTGCAGAGG CAGCATGTGT GGGCAAGAGC CAAAGTCCCA CGTGTGCACG 
TGTGCCGAGG GATACACTTT AAGCCAAGAT GGAAGGAAGT GTGAAGATGT CAACGAGTGT 
GCCTTCTGGA ATCATGGCTG TACTCTCGGG TGTGAAAACA CCCCTGGATC CTATTATTGC 
ACGTGTCCTG CAGGATTTGT TCTGCTCCCT GATGGGAAAC GGTGTCATCA ATTAATTTCC 
TGCCCAAGCA ATGTGTCTGA ATGTAGCCAT GACTGTGTTC TGACATCAGA TGGTCCCATA 
TGTTTCTGTC CTGAAGACTC AGTACTTGAG GCAGATGGAA AAACATGTAG CGGCTGTTCA 
TCACCTGATA ATGGTGGGTG CAGCCAGCTC TGCCTTCCTC TCAGTCCAGT AACCTGGGAA 
TGTGGCTGCT TTCCTGGGTA TGACCTACAG TTGGACAAAA AGAGCTGTAG AGCTTCAGGA 
CCACCACCGT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGACGGA 
ACAGATTATG AAACTCTGCT CAACCAGCAA ATAGGAATGG TTTTGGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGGTATA CTTTGCCCAT ACAGCCCTGA AATGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTTTC GAGGAAGCAG TCGATGTGCC AGAAGGTCTT 
GCCATAGACT GGATTGGCCG TAAATTCTAT TGGACAGACA GAGGGAGATC TCTCATTGAA 
GGGAGTGATT TAAATGGAAA ATACCGCGAA ATAATCATTA AGGAAGACAT CTCTCAGCCA 
CGAGGAATTG CTGTTCATCC AGTGGCCAAG AGATTATTCT GGACTGATAT GGGGACTAAT 
CCACGAATTG AAAGTTCATC CCTTCAAGGC ATTGGCCGAC AGGTTATAGC CAGCTCGGAT 
CTGGTCTGGC CCAGCGGAAT AACAATTGAT TACTTAACTG ACAAGTTGTA TTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGTCCAAT CTGGATGGTT CGAGACGCCA GAGACTTGCC 
CAGAACGATG TAGGTCACCC ATTTGCGGTA GCTGTGTTTG AGGATCACGT GTGGTTCTCT 
GACTGGACTA TGCCATCGGT AATAAGGGTG AACAAGAGGA CTGGCAAAAA TAGGGTACGT 
CTCCGAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTAG TTCATCCATT GGCAAAACCA 
GGAACAAATC CCTGCTTACA CCAAAATGGA GGCTGTGAAC ATATCTGCAA AGAGAGTTTT 
GGAACTGCTC AGTGTTTGTG TCATGAAGGT TTTCTGAAAG CCCCAGATGG AAAAATGTGT 
CTGGCTCTGA ATGGCCAAGA GATATTGGCA GGTCGTGGGA AAGATCTGAG TGATGGAGTA 
ATGCCAGTGG ACACCTTACC CAGAAGCAGA GAGCTTGAAG ATAACCTTAC AGAATCTCAA 
CACATTCTAG TGGCTGAAAT CATGGTTTCA GATGACGAGG ACTGCGGCGC TGCGGGATGC 
AGTGCGCAGG CGCGGTGTGT TACAGAGGGA GAGGACGCCA CGTGTCAGTG TTTGAAAGGA 
TTTGCTGGAG ATGGAAACCT GTGTTCTGAT ATAGATGAGT GTGAGCTGGG CACCTCGGTG 
TGCCCTCCTA CCTCCTCCGA GTGCATCAAT ACCGAAGGTG GTCACGTCTG CCGCTGCTCG 
GAAGGCTACC AGGGAGATGG GATCCACTGT CTCGATATTG ATGAGTGCCA ACTAGGTGTG 
CACACCTGTG GGGAAAATGC CACCTGTACA AATACGGAGG GAAACTACAC CTGCACATGT 
GCTGGCCGCC CCTCTGAACC CGGACGGATT TGCCCTGACC CTACTCCACC CTCTCACCTC 
GGGGAGGATG GCCGCTATTC TGTGAGAAAT AGTTACTCTG AATGCCCGCC GTCCCACGAC 
GGGTACTGCC TCCACGGTGG TGTGTGTATG TATATTGAAG CCGTCAACAG CTATGCCTGC 
AAGACTCAGA AGCTGCTATC AAAAAATCCA AAGAATCCTT ATGAAGAGTC AGGCAGAGAT 
GTGAGTGGTA TCAGGCCTGC AGACGGCGAG GCTGGGATGT CCTCTTGCCC CCAACCTTGG 
TTTGTGGTGA TAAAGGAACA TCAAAATCTC AGGAATGGGA GTCAACCTGG AGCCCCCAAG 
GACGGCCTGG GAGCAGATGT TGGCCAGTTT TCTTCCCTGG AGCCTGGGTC ATTGCAGCCG 
ACATCATGGA GGAAGGAACC CCAGATGTAT ATGGACACAG AACAAGGATG CTGTATTCCA 
TCCTCCAGTG ATAAAGGCTC TGGTCCCCAG GGAATAGGGT ATAGCTTTCA TCTCCCCTCC 
TATGGGGCAC GGTCCATTGC TGTCGGGGTT GAAAAATCCC ATTCTCTCCT GTCAGCTAAC 
CCATTACGGC AACAAAGGGC TCCAGATCCA CCACACCAAA TGGAGCTGAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020956120.1
CDS	487..3963
Translation	MLLFLILLLPVVLKFSFVSLSAPARWNCPEGSPSGNGNATCVGPAPFLIFSHGNSIFRIDLEGTNHEQLVADAGISVLMDFHYNEERIYWVDLERQLLQRVFLNGTRQEKVCNLEKNVSGMAINWINEELIWSNQQEGTITVTDMKGNNSRVLLSALKYPANVAVDPVERLMFWSSVVAGSLHRADVTGVEVRLLLETSEEIAAVSLDVLDKRLFWIQYNREGGSSRICSCDYDGGSVHFSKHLTQHNVFAMSLFGDHIFYSTWKKKTIWVANKHTGKDMVKMNLNPAFVPPGGIKVVHPLVQPKAEGDAWASDQKLCKLRKGNCRGSMCGQEPKSHVCTCAEGYTLSQDGRKCEDVNECAFWNHGCTLGCENTPGSYYCTCPAGFVLLPDGKRCHQLISCPSNVSECSHDCVLTSDGPICFCPEDSVLEADGKTCSGCSSPDNGGCSQLCLPLSPVTWECGCFPGYDLQLDKKSCRASGPPPFLLFANSQDIRHMHFDGTDYETLLNQQIGMVLALDHDPVENKVYFAHTALKWIERANMDGSQRERLFEEAVDVPEGLAIDWIGRKFYWTDRGRSLIEGSDLNGKYREIIIKEDISQPRGIAVHPVAKRLFWTDMGTNPRIESSSLQGIGRQVIASSDLVWPSGITIDYLTDKLYWCDAKQSVIEMSNLDGSRRQRLAQNDVGHPFAVAVFEDHVWFSDWTMPSVIRVNKRTGKNRVRLRGSMLKPSSLVVVHPLAKPGTNPCLHQNGGCEHICKESFGTAQCLCHEGFLKAPDGKMCLALNGQEILAGRGKDLSDGVMPVDTLPRSRELEDNLTESQHILVAEIMVSDDEDCGAAGCSAQARCVTEGEDATCQCLKGFAGDGNLCSDIDECELGTSVCPPTSSECINTEGGHVCRCSEGYQGDGIHCLDIDECQLGVHTCGENATCTNTEGNYTCTCAGRPSEPGRICPDPTPPSHLGEDGRYSVRNSYSECPPSHDGYCLHGGVCMYIEAVNSYACKTQKLLSKNPKNPYEESGRDVSGIRPADGEAGMSSCPQPWFVVIKEHQNLRNGSQPGAPKDGLGADVGQFSSLEPGSLQPTSWRKEPQMYMDTEQGCCIPSSSDKGSGPQGIGYSFHLPSYGARSIAVGVEKSHSLLSANPLRQQRAPDPPHQMELTQ

Target ORF information:

RefSeq Version	XM_021100461.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa epidermal growth factor (EGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021100461.1

ORF Insert Sequence:

ATGCTGCTCT TCCTTATCCT TCTGTTGCCG GTAGTTTTGA AATTTAGTTT TGTCAGCCTC 
TCAGCACCAG CGCGCTGGAA CTGTCCTGAA GGCTCTCCCT CAGGAAACGG GAATGCCACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ACAGTATCTT TAGGATTGAC 
TTGGAAGGAA CTAATCATGA GCAATTGGTG GCAGATGCTG GAATATCAGT GCTCATGGAC 
TTCCATTACA ATGAGGAAAG AATCTATTGG GTCGATCTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAAA GTATGCAATC TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGCACCATT 
ACAGTAACAG ATATGAAAGG AAACAATTCC CGTGTTCTCT TAAGCGCCTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTGATGTTTT GGTCTTCAGT GGTGGCCGGC 
AGCCTTCACA GGGCAGATGT TACAGGTGTG GAAGTGAGAC TTCTGTTGGA GACATCAGAG 
GAAATTGCAG CTGTGTCATT GGATGTGCTT GATAAACGGC TCTTTTGGAT TCAGTATAAC 
AGAGAAGGAG GCAGTTCTCG TATTTGTTCC TGTGATTATG ATGGAGGTTC TGTCCATTTT 
AGCAAACATC TCACACAGCA CAATGTGTTT GCAATGTCCC TTTTTGGTGA TCACATCTTC 
TATTCAACAT GGAAAAAGAA GACAATTTGG GTAGCTAACA AGCACACTGG GAAGGATATG 
GTTAAAATGA ACCTCAATCC AGCATTTGTA CCACCTGGTG GAATTAAAGT AGTGCATCCA 
CTTGTTCAGC CCAAGGCAGA GGGTGATGCT TGGGCCTCTG ATCAGAAACT CTGCAAACTG 
AGGAAGGGTA ACTGCAGAGG CAGCATGTGT GGGCAAGAGC CAAAGTCCCA CGTGTGCACG 
TGTGCCGAGG GATACACTTT AAGCCAAGAT GGAAGGAAGT GTGAAGATGT CAACGAGTGT 
GCCTTCTGGA ATCATGGCTG TACTCTCGGG TGTGAAAACA CCCCTGGATC CTATTATTGC 
ACGTGTCCTG CAGGATTTGT TCTGCTCCCT GATGGGAAAC GGTGTCATCA ATTAATTTCC 
TGCCCAAGCA ATGTGTCTGA ATGTAGCCAT GACTGTGTTC TGACATCAGA TGGTCCCATA 
TGTTTCTGTC CTGAAGACTC AGTACTTGAG GCAGATGGAA AAACATGTAG CGGCTGTTCA 
TCACCTGATA ATGGTGGGTG CAGCCAGCTC TGCCTTCCTC TCAGTCCAGT AACCTGGGAA 
TGTGGCTGCT TTCCTGGGTA TGACCTACAG TTGGACAAAA AGAGCTGTAG AGCTTCAGGA 
CCACCACCGT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGACGGA 
ACAGATTATG AAACTCTGCT CAACCAGCAA ATAGGAATGG TTTTGGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGGTATA CTTTGCCCAT ACAGCCCTGA AATGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTTTC GAGGAAGCAG TCGATGTGCC AGAAGGTCTT 
GCCATAGACT GGATTGGCCG TAAATTCTAT TGGACAGACA GAGGGAGATC TCTCATTGAA 
GGGAGTGATT TAAATGGAAA ATACCGCGAA ATAATCATTA AGGAAGACAT CTCTCAGCCA 
CGAGGAATTG CTGTTCATCC AGTGGCCAAG AGATTATTCT GGACTGATAT GGGGACTAAT 
CCACGAATTG AAAGTTCATC CCTTCAAGGC ATTGGCCGAC AGGTTATAGC CAGCTCGGAT 
CTGGTCTGGC CCAGCGGAAT AACAATTGAT TACTTAACTG ACAAGTTGTA TTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGTCCAAT CTGGATGGTT CGAGACGCCA GAGACTTGCC 
CAGAACGATG TAGGTCACCC ATTTGCGGTA GCTGTGTTTG AGGATCACGT GTGGTTCTCT 
GACTGGACTA TGCCATCGGT AATAAGGGTG AACAAGAGGA CTGGCAAAAA TAGGGTACGT 
CTCCGAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTAG TTCATCCATT GGCAAAACCA 
GGAACAAATC CCTGCTTACA CCAAAATGGA GGCTGTGAAC ATATCTGCAA AGAGAGTTTT 
GGAACTGCTC AGTGTTTGTG TCATGAAGGT TTTCTGAAAG CCCCAGATGG AAAAATGTGT 
CTGGCTCTGA ATGGCCAAGA GATATTGGCA GGTCGTGGGA AAGATCTGAG TGATGGAGTA 
ATGCCAGTGG ACACCTTACC CAGAAGCAGA GAGCTTGAAG ATAACCTTAC AGAATCTCAA 
CACATTCTAG TGGCTGAAAT CATGGTTTCA GATGACGAGG ACTGCGGCGC TGCGGGATGC 
AGTGCGCAGG CGCGGTGTGT TACAGAGGGA GAGGACGCCA CGTGTCAGTG TTTGAAAGGA 
TTTGCTGGAG ATGGAAACCT GTGTTCTGAT ATAGATGAGT GTGAGCTGGG CACCTCGGTG 
TGCCCTCCTA CCTCCTCCGA GTGCATCAAT ACCGAAGGTG GTCACGTCTG CCGCTGCTCG 
GAAGGCTACC AGGGAGATGG GATCCACTGT CTCGATATTG ATGAGTGCCA ACTAGGTGTG 
CACACCTGTG GGGAAAATGC CACCTGTACA AATACGGAGG GAAACTACAC CTGCACATGT 
GCTGGCCGCC CCTCTGAACC CGGACGGATT TGCCCTGACC CTACTCCACC CTCTCACCTC 
GGGGAGGATG GCCGCTATTC TGTGAGAAAT AGTTACTCTG AATGCCCGCC GTCCCACGAC 
GGGTACTGCC TCCACGGTGG TGTGTGTATG TATATTGAAG CCGTCAACAG CTATGCCTGC 
AAGACTCAGA AGCTGCTATC AAAAAATCCA AAGAATCCTT ATGAAGAGTC AGGCAGAGAT 
GTGAGTGGTA TCAGGCCTGC AGACGGCGAG GCTGGGATGT CCTCTTGCCC CCAACCTTGG 
TTTGTGGTGA TAAAGGAACA TCAAAATCTC AGGAATGGGA GTCAACCTGG AGCCCCCAAG 
GACGGCCTGG GAGCAGATGT TGGCCAGTTT TCTTCCCTGG AGCCTGGGTC ATTGCAGCCG 
ACATCATGGA GGAAGGAACC CCAGATGTAT ATGGACACAG AACAAGGATG CTGTATTCCA 
TCCTCCAGTG ATAAAGGCTC TGGTCCCCAG GGAATAGGGT ATAGCTTTCA TCTCCCCTCC 
TATGGGGCAC GGTCCATTGC TGTCGGGGTT GAAAAATCCC ATTCTCTCCT GTCAGCTAAC 
CCATTACGGC AACAAAGGGC TCCAGATCCA CCACACCAAA TGGAGCTGAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_021100462.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020956121.1 Sus scrofa epidermal growth factor (EGF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe223946
Clone ID Related Accession (Same CDS sequence)	XM_021100462.1
Accession Version	XM_021100462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	pro-epidermal growth factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCT TCCTTATCCT TCTGTTGCCG GTAGTTTTGA AATTTAGTTT TGTCAGCCTC 
TCAGCACCAG CGCGCTGGAA CTGTCCTGAA GGCTCTCCCT CAGGAAACGG GAATGCCACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ACAGTATCTT TAGGATTGAC 
TTGGAAGGAA CTAATCATGA GCAATTGGTG GCAGATGCTG GAATATCAGT GCTCATGGAC 
TTCCATTACA ATGAGGAAAG AATCTATTGG GTCGATCTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAAA GTATGCAATC TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGCACCATT 
ACAGTAACAG ATATGAAAGG AAACAATTCC CGTGTTCTCT TAAGCGCCTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTGATGTTTT GGTCTTCAGT GGTGGCCGGC 
AGCCTTCACA GGGCAGATGT TACAGGTGTG GAAGTGAGAC TTCTGTTGGA GACATCAGAG 
GAAATTGCAG CTGTGTCATT GGATGTGCTT GATAAACGGC TCTTTTGGAT TCAGTATAAC 
AGAGAAGGAG GCAGTTCTCG TATTTGTTCC TGTGATTATG ATGGAGGTTC TGTCCATTTT 
AGCAAACATC TCACACAGCA CAATGTGTTT GCAATGTCCC TTTTTGGTGA TCACATCTTC 
TATTCAACAT GGAAAAAGAA GACAATTTGG GTAGCTAACA AGCACACTGG GAAGGATATG 
GTTAAAATGA ACCTCAATCC AGCATTTGTA CCACCTGGTG GAATTAAAGT AGTGCATCCA 
CTTGTTCAGC CCAAGGCAGA GGGTGATGCT TGGGCCTCTG ATCAGAAACT CTGCAAACTG 
AGGAAGGGTA ACTGCAGAGG CAGCATGTGT GGGCAAGAGC CAAAGTCCCA CGTGTGCACG 
TGTGCCGAGG GATACACTTT AAGCCAAGAT GGAAGGAAGT GTGAAGATGT CAACGAGTGT 
GCCTTCTGGA ATCATGGCTG TACTCTCGGG TGTGAAAACA CCCCTGGATC CTATTATTGC 
ACGTGTCCTG CAGGATTTGT TCTGCTCCCT GATGGGAAAC GGTGTCATCA ATTAATTTCC 
TGCCCAAGCA ATGTGTCTGA ATGTAGCCAT GACTGTGTTC TGACATCAGA TGGTCCCATA 
TGTTTCTGTC CTGAAGACTC AGTACTTGAG GCAGATGGAA AAACATGTAG CGGCTGTTCA 
TCACCTGATA ATGGTGGGTG CAGCCAGCTC TGCCTTCCTC TCAGTCCAGT AACCTGGGAA 
TGTGGCTGCT TTCCTGGGTA TGACCTACAG TTGGACAAAA AGAGCTGTAG AGCTTCAGGA 
CCACCACCGT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGACGGA 
ACAGATTATG AAACTCTGCT CAACCAGCAA ATAGGAATGG TTTTGGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGGTATA CTTTGCCCAT ACAGCCCTGA AATGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTTTC GAGGAAGCAG TCGATGTGCC AGAAGGTCTT 
GCCATAGACT GGATTGGCCG TAAATTCTAT TGGACAGACA GAGGGAGATC TCTCATTGAA 
GGGAGTGATT TAAATGGAAA ATACCGCGAA ATAATCATTA AGGAAGACAT CTCTCAGCCA 
CGAGGAATTG CTGTTCATCC AGTGGCCAAG AGATTATTCT GGACTGATAT GGGGACTAAT 
CCACGAATTG AAAGTTCATC CCTTCAAGGC ATTGGCCGAC AGGTTATAGC CAGCTCGGAT 
CTGGTCTGGC CCAGCGGAAT AACAATTGAT TACTTAACTG ACAAGTTGTA TTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGTCCAAT CTGGATGGTT CGAGACGCCA GAGACTTGCC 
CAGAACGATG TAGGTCGTGG GAAAGATCTG AGTGATGGAG TAATGCCAGT GGACACCTTA 
CCCAGAAGCA GAGAGCTTGA AGATAACCTT ACAGAATCTC AACACATTCT AGTGGCTGAA 
ATCATGGTTT CAGATGACGA GGACTGCGGC GCTGCGGGAT GCAGTGCGCA GGCGCGGTGT 
GTTACAGAGG GAGAGGACGC CACGTGTCAG TGTTTGAAAG GATTTGCTGG AGATGGAAAC 
CTGTGTTCTG ATATAGATGA GTGTGAGCTG GGCACCTCGG TGTGCCCTCC TACCTCCTCC 
GAGTGCATCA ATACCGAAGG TGGTCACGTC TGCCGCTGCT CGGAAGGCTA CCAGGGAGAT 
GGGATCCACT GTCTCGATAT TGATGAGTGC CAACTAGGTG TGCACACCTG TGGGGAAAAT 
GCCACCTGTA CAAATACGGA GGGAAACTAC ACCTGCACAT GTGCTGGCCG CCCCTCTGAA 
CCCGGACGGA TTTGCCCTGA CCCTACTCCA CCCTCTCACC TCGGGGAGGA TGGCCGCTAT 
TCTGTGAGAA ATAGTTACTC TGAATGCCCG CCGTCCCACG ACGGGTACTG CCTCCACGGT 
GGTGTGTGTA TGTATATTGA AGCCGTCAAC AGCTATGCCT GCAACTGTGT TTTTGGCTAC 
GTTGGCGAGC GATGTCAGCA CAGAGACTTG AAATGGTGGG AGCTGCGCCA CGCTGGCCTC 
GGGCGACAGT GGAACGTCAC GGTGGTGGCC GTCTGCGTGG TGGTGCTGGT CCTGCTGCTG 
CTCCTGGGGC TGTGGGGGGC TCACTACTAC AGGACTCAGA AGCTGCTATC AAAAAATCCA 
AAGAATCCTT ATGAAGAGTC AGGCAGAGAT GTGAGTGGTA TCAGGCCTGC AGACGGCGAG 
GCTGGGATGT CCTCTTGCCC CCAACCTTGG TTTGTGGTGA TAAAGGAACA TCAAAATCTC 
AGGAATGGGA GTCAACCTGG AGCCCCCAAG GACGGCCTGG GAGCAGATGT TGGCCAGTTT 
TCTTCCCTGG AGCCTGGGTC ATTGCAGCCG ACATCATGGA GGAAGGAACC CCAGATGTAT 
ATGGACACAG AACAAGGATG CTGTATTCCA TCCTCCAGTG ATAAAGGCTC TGGTCCCCAG 
GGAATAGGGT ATAGCTTTCA TCTCCCCTCC TATGGGGCAC GGTCCATTGC TGTCGGGGTT 
GAAAAATCCC ATTCTCTCCT GTCAGCTAAC CCATTACGGC AACAAAGGGC TCCAGATCCA 
CCACACCAAA TGGAGCTGAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020956121.1
CDS	487..3813
Translation	MLLFLILLLPVVLKFSFVSLSAPARWNCPEGSPSGNGNATCVGPAPFLIFSHGNSIFRIDLEGTNHEQLVADAGISVLMDFHYNEERIYWVDLERQLLQRVFLNGTRQEKVCNLEKNVSGMAINWINEELIWSNQQEGTITVTDMKGNNSRVLLSALKYPANVAVDPVERLMFWSSVVAGSLHRADVTGVEVRLLLETSEEIAAVSLDVLDKRLFWIQYNREGGSSRICSCDYDGGSVHFSKHLTQHNVFAMSLFGDHIFYSTWKKKTIWVANKHTGKDMVKMNLNPAFVPPGGIKVVHPLVQPKAEGDAWASDQKLCKLRKGNCRGSMCGQEPKSHVCTCAEGYTLSQDGRKCEDVNECAFWNHGCTLGCENTPGSYYCTCPAGFVLLPDGKRCHQLISCPSNVSECSHDCVLTSDGPICFCPEDSVLEADGKTCSGCSSPDNGGCSQLCLPLSPVTWECGCFPGYDLQLDKKSCRASGPPPFLLFANSQDIRHMHFDGTDYETLLNQQIGMVLALDHDPVENKVYFAHTALKWIERANMDGSQRERLFEEAVDVPEGLAIDWIGRKFYWTDRGRSLIEGSDLNGKYREIIIKEDISQPRGIAVHPVAKRLFWTDMGTNPRIESSSLQGIGRQVIASSDLVWPSGITIDYLTDKLYWCDAKQSVIEMSNLDGSRRQRLAQNDVGRGKDLSDGVMPVDTLPRSRELEDNLTESQHILVAEIMVSDDEDCGAAGCSAQARCVTEGEDATCQCLKGFAGDGNLCSDIDECELGTSVCPPTSSECINTEGGHVCRCSEGYQGDGIHCLDIDECQLGVHTCGENATCTNTEGNYTCTCAGRPSEPGRICPDPTPPSHLGEDGRYSVRNSYSECPPSHDGYCLHGGVCMYIEAVNSYACNCVFGYVGERCQHRDLKWWELRHAGLGRQWNVTVVAVCVVVLVLLLLLGLWGAHYYRTQKLLSKNPKNPYEESGRDVSGIRPADGEAGMSSCPQPWFVVIKEHQNLRNGSQPGAPKDGLGADVGQFSSLEPGSLQPTSWRKEPQMYMDTEQGCCIPSSSDKGSGPQGIGYSFHLPSYGARSIAVGVEKSHSLLSANPLRQQRAPDPPHQMELTQ

Target ORF information:

RefSeq Version	XM_021100462.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa epidermal growth factor (EGF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021100462.1

ORF Insert Sequence:

ATGCTGCTCT TCCTTATCCT TCTGTTGCCG GTAGTTTTGA AATTTAGTTT TGTCAGCCTC 
TCAGCACCAG CGCGCTGGAA CTGTCCTGAA GGCTCTCCCT CAGGAAACGG GAATGCCACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ACAGTATCTT TAGGATTGAC 
TTGGAAGGAA CTAATCATGA GCAATTGGTG GCAGATGCTG GAATATCAGT GCTCATGGAC 
TTCCATTACA ATGAGGAAAG AATCTATTGG GTCGATCTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAAA GTATGCAATC TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGCACCATT 
ACAGTAACAG ATATGAAAGG AAACAATTCC CGTGTTCTCT TAAGCGCCTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTGATGTTTT GGTCTTCAGT GGTGGCCGGC 
AGCCTTCACA GGGCAGATGT TACAGGTGTG GAAGTGAGAC TTCTGTTGGA GACATCAGAG 
GAAATTGCAG CTGTGTCATT GGATGTGCTT GATAAACGGC TCTTTTGGAT TCAGTATAAC 
AGAGAAGGAG GCAGTTCTCG TATTTGTTCC TGTGATTATG ATGGAGGTTC TGTCCATTTT 
AGCAAACATC TCACACAGCA CAATGTGTTT GCAATGTCCC TTTTTGGTGA TCACATCTTC 
TATTCAACAT GGAAAAAGAA GACAATTTGG GTAGCTAACA AGCACACTGG GAAGGATATG 
GTTAAAATGA ACCTCAATCC AGCATTTGTA CCACCTGGTG GAATTAAAGT AGTGCATCCA 
CTTGTTCAGC CCAAGGCAGA GGGTGATGCT TGGGCCTCTG ATCAGAAACT CTGCAAACTG 
AGGAAGGGTA ACTGCAGAGG CAGCATGTGT GGGCAAGAGC CAAAGTCCCA CGTGTGCACG 
TGTGCCGAGG GATACACTTT AAGCCAAGAT GGAAGGAAGT GTGAAGATGT CAACGAGTGT 
GCCTTCTGGA ATCATGGCTG TACTCTCGGG TGTGAAAACA CCCCTGGATC CTATTATTGC 
ACGTGTCCTG CAGGATTTGT TCTGCTCCCT GATGGGAAAC GGTGTCATCA ATTAATTTCC 
TGCCCAAGCA ATGTGTCTGA ATGTAGCCAT GACTGTGTTC TGACATCAGA TGGTCCCATA 
TGTTTCTGTC CTGAAGACTC AGTACTTGAG GCAGATGGAA AAACATGTAG CGGCTGTTCA 
TCACCTGATA ATGGTGGGTG CAGCCAGCTC TGCCTTCCTC TCAGTCCAGT AACCTGGGAA 
TGTGGCTGCT TTCCTGGGTA TGACCTACAG TTGGACAAAA AGAGCTGTAG AGCTTCAGGA 
CCACCACCGT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGACGGA 
ACAGATTATG AAACTCTGCT CAACCAGCAA ATAGGAATGG TTTTGGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGGTATA CTTTGCCCAT ACAGCCCTGA AATGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTTTC GAGGAAGCAG TCGATGTGCC AGAAGGTCTT 
GCCATAGACT GGATTGGCCG TAAATTCTAT TGGACAGACA GAGGGAGATC TCTCATTGAA 
GGGAGTGATT TAAATGGAAA ATACCGCGAA ATAATCATTA AGGAAGACAT CTCTCAGCCA 
CGAGGAATTG CTGTTCATCC AGTGGCCAAG AGATTATTCT GGACTGATAT GGGGACTAAT 
CCACGAATTG AAAGTTCATC CCTTCAAGGC ATTGGCCGAC AGGTTATAGC CAGCTCGGAT 
CTGGTCTGGC CCAGCGGAAT AACAATTGAT TACTTAACTG ACAAGTTGTA TTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGTCCAAT CTGGATGGTT CGAGACGCCA GAGACTTGCC 
CAGAACGATG TAGGTCGTGG GAAAGATCTG AGTGATGGAG TAATGCCAGT GGACACCTTA 
CCCAGAAGCA GAGAGCTTGA AGATAACCTT ACAGAATCTC AACACATTCT AGTGGCTGAA 
ATCATGGTTT CAGATGACGA GGACTGCGGC GCTGCGGGAT GCAGTGCGCA GGCGCGGTGT 
GTTACAGAGG GAGAGGACGC CACGTGTCAG TGTTTGAAAG GATTTGCTGG AGATGGAAAC 
CTGTGTTCTG ATATAGATGA GTGTGAGCTG GGCACCTCGG TGTGCCCTCC TACCTCCTCC 
GAGTGCATCA ATACCGAAGG TGGTCACGTC TGCCGCTGCT CGGAAGGCTA CCAGGGAGAT 
GGGATCCACT GTCTCGATAT TGATGAGTGC CAACTAGGTG TGCACACCTG TGGGGAAAAT 
GCCACCTGTA CAAATACGGA GGGAAACTAC ACCTGCACAT GTGCTGGCCG CCCCTCTGAA 
CCCGGACGGA TTTGCCCTGA CCCTACTCCA CCCTCTCACC TCGGGGAGGA TGGCCGCTAT 
TCTGTGAGAA ATAGTTACTC TGAATGCCCG CCGTCCCACG ACGGGTACTG CCTCCACGGT 
GGTGTGTGTA TGTATATTGA AGCCGTCAAC AGCTATGCCT GCAACTGTGT TTTTGGCTAC 
GTTGGCGAGC GATGTCAGCA CAGAGACTTG AAATGGTGGG AGCTGCGCCA CGCTGGCCTC 
GGGCGACAGT GGAACGTCAC GGTGGTGGCC GTCTGCGTGG TGGTGCTGGT CCTGCTGCTG 
CTCCTGGGGC TGTGGGGGGC TCACTACTAC AGGACTCAGA AGCTGCTATC AAAAAATCCA 
AAGAATCCTT ATGAAGAGTC AGGCAGAGAT GTGAGTGGTA TCAGGCCTGC AGACGGCGAG 
GCTGGGATGT CCTCTTGCCC CCAACCTTGG TTTGTGGTGA TAAAGGAACA TCAAAATCTC 
AGGAATGGGA GTCAACCTGG AGCCCCCAAG GACGGCCTGG GAGCAGATGT TGGCCAGTTT 
TCTTCCCTGG AGCCTGGGTC ATTGCAGCCG ACATCATGGA GGAAGGAACC CCAGATGTAT 
ATGGACACAG AACAAGGATG CTGTATTCCA TCCTCCAGTG ATAAAGGCTC TGGTCCCCAG 
GGAATAGGGT ATAGCTTTCA TCTCCCCTCC TATGGGGCAC GGTCCATTGC TGTCGGGGTT 
GAAAAATCCC ATTCTCTCCT GTCAGCTAAC CCATTACGGC AACAAAGGGC TCCAGATCCA 
CCACACCAAA TGGAGCTGAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association and polymorphism study of seven candidate genes with reproductive traits in three pig breeds in Hungary.
Acta biochimica Polonica63(2)359-64(2016)
Hunyadi-Bagi Á,Balogh P,Nagy K,Kusza S

Epidermal growth factor can optimize a serum-free culture system for bone marrow stem cell proliferation in a miniature pig model.
In vitro cellular & developmental biology. Animal49(10)815-25(2013 Dec)
Wang X,Zheng F,Liu O,Zheng S,Liu Y,Wang Y,Tang Z,Zhong L

Epidermal growth factor stimulates proliferation and migration of porcine trophectoderm cells through protooncogenic protein kinase 1 and extracellular-signal-regulated kinases 1/2 mitogen-activated protein kinase signal transduction cascades during early pregnancy.
Molecular and cellular endocrinology381(1-2)302-11(2013 Dec)
Jeong W,Kim J,Bazer FW,Song G

Progesterone is essential for maintenance of Tace/Adam17 mRNA expression, but not EGF-like factor, in cumulus cells, which enhances the EGF receptor signaling pathway during in vitro maturation of porcine COCs.
The Journal of reproduction and development56(3)315-23(2010 Jun)
Yamashita Y,Kawashima I,Gunji Y,Hishinuma M,Shimada M

Opposing roles of EGF in IFN-alpha-induced epithelial barrier destabilization and tissue repair.
American journal of physiology. Cell physiology293(6)C1843-50(2007 Dec)
Lechner J,Malloth NA,Jennings P,Heckl D,Pfaller W,Seppi T

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EGF cDNA ORF clone, Sus scrofa(pig)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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