LOC581149 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC581149
Entrez Gene ID	581149
Full Name	uncharacterized LOC581149
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030999829.1	XP_030855689.1	YLP motif-containing protein 1 isoform X2
XM_781163.4	XP_786256.3	uncharacterized protein LOC581149
XM_030999828.1	XP_030855688.1	YLP motif-containing protein 1 isoform X1
XM_030999830.1	XP_030855690.1	YLP motif-containing protein 1 isoform X3
XM_030999831.1	XP_030855691.1	YLP motif-containing protein 1 isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC581149 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC581149 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd07467	XM_781163.4 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC581149 (LOC581149), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSd65788	XM_030999828.1 Latest version!	Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSd65789	XM_030999829.1 Latest version!	Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSd65790	XM_030999830.1 Latest version!	Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSd65791	XM_030999831.1 Latest version!	Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC581149 ( XM_781163.4 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_786256.3 Strongylocentrotus purpuratus uncharacterized LOC581149 (LOC581149), mRNA.

Price & Availability↑

CloneID	OSd07467
Clone ID Related Accession (Same CDS sequence)	XM_781163.4
Accession Version	XM_781163.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC581149
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011989134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_781163.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GATCAGGTAT GAATATGATG ATGGGACCAG GTGGACCAGG ACCAAGAAGA 
CCACCTCCAC CGATGTCGAT GATGCATGGT CCAGGTCCAG GTGGCCCAAG GCCAAGAATG 
GGTATGCCCA TCCCAGAGCC TGTTCGGGTG TTAGTCAATC AAGCGCAAGC CGCAGATACT 
GCAGTCCCAC AACCGCCTCA GAATGAAGAT GTGAAGTTGA GAGGGCTTCA GGATCAGGCC 
TTACATTGGC AGGCAACGGC CCCGGTTCGA AAGGAGGAAG AAACTGTACA AAAACAGGCA 
GCTGCTCAAG ACCAGTGGCA GAGCTACCAG CAACAGATGC AATCATATCA GCAACAGTAT 
CAACAATGGT ACCAAAGTCA AGAGACTACC TTGATGGATG TTAAATTGAA AGACTTGCAG 
GAAAAGAAGG CTACCTGTGA AAAGCAGCTC ACAGACTGGA AAAATCAGTT TGATAATTGG 
GTAGAACAGC ATAAGAAACA TCCAAACAAG GACCAGTTTG ATCAATATCA AAATCAATGG 
AAAACATGGC AAACACAGAT GGAGAGCATG GTGAATTCTT TGGACATGCA AATCAAAATA 
AAAGAGACAT CCCTTCAGAA GAACTCCTCT AAAAATGCTT CAACAACCCA GACACCTGCT 
TCTGTCGGCA GTGGTGTAGG AACAGTTACC ACACAAGGTG CAAATGTAGC AATGCCATCA 
GGTGGGCATG GAATGGTTCC ATCTAGTGGT GCTGGAATGG GACAAGGGTC ATCAATTGGT 
TCTATACCAA GCAGTCAGAG TGTAGCCACA GTTCCAAGTG GTTCTGGGAT GGGAGCAGGA 
CCCAGAGGAC TTGGAGTAGG ACCAATGCCA ACTGGGAGTG GAATGGGACC AGGTCCCTCA 
GGAACAAATC CTAGAGTTAC CCCTGCATTG CCTCAAGGAC CTGGGCAAGG GCCAAGAATG 
GAGAGTGGAA GCAGGTCAAA CTCTGGAGCT GGACTTCCAG GCCCAAAAGG ACAGATGCCT 
GGCAAAGACA GCTTTCCTTT CCCATTTCCT GATGATTTTC CATTTCCAGG AGATATGGAC 
AATTTCTCCT TCCCTGATAT GAATAAACTA GATGCAATGT TTTCAGGTGG TTTTAAATCT 
GATGAGTTTC CATTTTCCTT CCCTGATTTT CCGATGCCAG GTGGTTTAAT GGGACCCGAC 
TTTCCATTAC CTGAAGGACT GAAACCCGAA GACATGACCA AAGATATGGA AGAATTCATG 
AAACAGATGG AATCTGAACT AGGCAATGTT TCTGCAAAAT TTCAACCAAC CCCAAGGGGA 
AGCAACATAG CCGATCAGGA TAAACAGCAA GCTTTAAATG CCAAAGCATC AAGAGGACCT 
GTAGACATAG AGAGGGGTCC ACAAGGAATG AACAGAGATT CTGCTGGGAT GGGAAGACAC 
CCAATGGGAA TGGATAGAGG TCCTCAAGGT ATGGAGAGAC ACTCACAAGA ATTCAATAGA 
GGTCTATCAG GAATAGACAG AGGCCCAAGA GAAAGTCTTC AAGGGAGGGA AAGGGTCCCA 
CCAGGAATGG ATAGAGGACA ACAAGGAATG GATAGATGCC CTCCCGGAAT GGATAGAAGA 
AGACCAGGCA TGGACGGAGG CCCTCTTGGA ATGGATAGAG GACAACCAGG AATGGATAGA 
GGACAACCAG GAATGGACAG AGGCCCTCTT GGAATGGATA GAGGACAACC AGAAATGGAC 
AGAGGCCCTC TTGGAATGGA TAGAGGACAA CCAGGAATTG ACGGAGGCCC TCTTGGAATG 
AATAGAGGAC AACCAGGAAT GGATAGAGGC ACTCCTGGAA TGGATAGAGG TCCCCCTGGG 
ATGGATAGGG GACCAACCGG AATGGATAGA GGACAACCAA GCTTGGATAG AGGTCCTCCT 
GGAATGGATA GAGGACAGCA AGGAATGGAT AGAGGTCAAC CAGGAATGGA TAGAGGACAA 
CCAGGAATGG ATAGAGGACA ACCAGGAATG GATAGAGGTC CTCCTGGAAT GGATAGAGGA 
CAGCAACGAA TGGATAGAGG ACAGCAAGGA ATGGATAGAG GTTATCCTGG AATGGATAGA 
GGACAAACAG GAATGGATAG AGGTCCTCCT GGAATGGATA GAGGACAGCA AGGAATGGAG 
AGAGTTCCTC CTGGAACAGA CAAAAGACTA CCCGGAATGG ATAGAGGCCC TCCTGGAATG 
GATGGAGGCC CTCCCAGACT GGATAGAGGA CAACCAGGCA TGGATAAAGG CCCTCCTGGA 
ATGAATAGAG GCCCTCCCGG AATGGATGGA GGCCCTCCCG GAATGGATAG AGGACATCCA 
GGCATGGATA AAGGCCCTCC TGGAATGGAT AGAGGCCCTC CCGGAATGGA GAGAGGCCCA 
CCTGGACTGG ATAGAGGACA TCCAGGCATG GATAAAGGCC CTCCTGGAAT GGAGAGTGGA 
CATCCAGGCA TAGATAAAGG CCCTCCTGGA ATGGATAGAG GTACAGCAGG AAGGAATAGA 
GGCCAATCAG AAATGGATAG AGGCTCATCA AGAAATGAGG GAGATGCACC AAGAACTAAT 
AAAGTTCAAC TAGGGATCGG GGGAATTCTT GCAGGACTTA AAGATTCATC AGGTATGGAT 
AATGATCATA GAATGATTGA CAGAGGCCCA CCAGGAATTG ACAGTGGCCC TCCAGGAGCA 
GACAGTGGCC CACCAGGAGT AGACAGTGGT GCATTGAGAA CCGATAGAAG TCAACTAGCT 
TTAGGGAACG TTCTTGCTGG GTTGAGGGCA GCCCCACCAG GAATGGATAG AGGCCCACCA 
GGAATAGACG GTGGCCCACC AGGAATAGAC AGTGGCCCAC CAGGAATAGA AATAAACGCC 
CCAGGTCGTA CTAAAAGTCA ACTAGGTTTA GGGAGCATTC TTGCTGCACT GAAGACAGGT 
CCACCAGGAA TGGATACGGG TCCACCTGGT GTGGAGATGA GACCTAGTGG CATGGACAAG 
GGACCCCCGG GAATAGACAT AGCCCCTCCA GTTATGGATA TGGGTCCTCC AGGAACGGAC 
AGAGGACCTC CATCTAATTA CATTAAACCT CCAGGGATGG ACAACTCACC TCCAGTTATG 
GGGACATTGC CCATGAGTTT GACCAGGGGT CCTCCTGGTC CACCTGGAGT TGATGTTGGA 
CCTCCTGGTG TCGATGTTGG ACCTCCTGGT GTGGATATTG ATGCAATTAG TATGCATAGC 
CATTATTTGG GAGCTGGAAA GCCTCGCCCT CCATTTGATC AAAGGCAATT GGATATTGGT 
CCACCAGGTG TAGATACCTT TCCCCCTGGA ATTCCACGTT ATCCTTTTGG TTCAACACAA 
GGTCCAGCAG GATTTGGAAA CTCATCTTCA ATTCAAGATA GCAGCTTTGA AGAGCCAATG 
CCTCCTGGTA TGGAGCCTAT TAGTAAACCC CTTGCTTTTC CCAGAGATCT TGCACCTCCT 
GGGATGGATG AGACATTTCC ACCAGGGATG GAACCAGAAC AGGAAAGCCA GCCAAGCACA 
AAAAGTAAAC AGCCGCAACC ACCGGAAGAC AATATAAGTG AATTTGAAAA GGGGCAAAAC 
AAGCCACTGT TTCCCCCACC ATCCAAAGGT TTGACTCAAC ATTCCTATTC AGTTGGACAA 
TCCATACCAA TTACAGGTAT GGTGAAAGAT TCTTTTGGTG CCAGCTCCTT TGTTTCAACA 
AAACAGACAT TAGCTCCAAG CCCACTTGCT GGGTATGAGT CATTAGAATC GGTAAAGGCC 
AAGAAAGAAG AAAAGAAAAA AGATGAATCA AAGTCAGAGG ATAAAAAAAA AGGAAAAGGG 
AAAAAGGGAA ATATGAACAT GCCTACAATC ATCAAATTTG GGGATTCTAA AATTGATAAA 
GATTCTGATG ATCCTGTCAA AGTTGAAAAG GACGAGACAT CTACTGATGA AAAAGCAGCA 
AAGGAGGCTG CAAAAGCTAA GGAGTTTAAA AAAGAAAATT CACCTTCTGA GGAGGTTTCA 
AAGAGAGTAA CTGTGCGTAG CACCCTACCA TTGATCACAG ATTCTACAAC CAAGTCTATG 
ATACCTTCAT CAATTGGTGA GCTATTCAAG AAGAACTCTA CAACACCTCC AGCATTCAAC 
AAAGATGTAA AGACCTCACT TAGGAGCTAC AGGAAAGCAG CAGAAGATGA TGAGGAAGAG 
GAAGAAGCGC CTCACTCTAA AAAGATGAAT AGGAAGGATT CTAGCGATAG AGATGATAGG 
GGACGGGATG ACACACTCCG CACTACACTC CCATCCCCCC CACGGTCATA TCGGTCACCT 
GATCGAAGGT CTAGGAGATC TCCTGAAAGA AGGAGATCTC GTTCTCCAGA CAAAAGAAAG 
CGTAGATCTC GGTCTAGATC GATCTCACAG GACCGCAGGA AACGGAGGCG GGATTCTCCA 
CCCCGTAAGA GAAACTCATA CTCTCCGAGC AAAAGGAGGT CCCCCTCTAA AAGTAAAAAT 
TCACGATCCC CAAGCAGAGA CAAGGGCAAT AGATCCCCGG ATCGAAGAGA TCAGAGAGAT 
CGAAGAGATC AGAGAGATCA AAGAGATCAG AGAGATCGAA GAGATGGGAG AGATGACAGA 
TACAGAGAAA AGGACAGGAG GGACAGGTAT CCTGACAAAG AATATGAGAA AGATTCTGAT 
AGGAGAGGTG GGAAGCCAAG TACAGAAGCC TTTGACAAGT TGCGAGAGCT GATTTATGAT 
ACCTTGGTGC GCCATCCTGA TGAAAAGCCA AGGGATTCTC CACATAGCGA TAGGCGACAC 
CCGGACGATA GATCTCCTCA GCGACCAAGA AGGGAGAACT ATTACCCGGA AGCTGAGAGG 
CCAAGAATGC GATATCCTCC ACCACGTAGA GACTATGAAA GAGATCCTTT GAGGGATCGG 
TTAAGAAGCC TTCCAAGGGA TTCACCAAGG GATCTTCCAT TGCCAGCTCA AAGGGAGCCT 
CGTAGGGATT TTGATAGAGA TTCCTCTCAA GACAGATCTA TGGAGAGGGC TCATTCCCCT 
CCGGTGACGG AAATAACAGC ACCTATTGTC TTTGACTATG GGCATGCAGA GTCCACCAGG 
CCTGCTTATG ACATAGAGAC AAGAGATCCC ATGGGAAGCC TGCTCAGCAA TGCAGTTATT 
GTAGACGTTG TTGATTATAG TCATGGTCAG AAGCAGGGAC CAGATGCTAG AGCAATTAGC 
GGATCAGAAC TTCTTCAAGA GCGGGAGTCA TTGTTCCCTT CAGAATTTCA AATACATAAA 
GAGATTCAAC CCGCAAAGAG AGATGATACC AGTATGCAAC AATCACCAGC TCCACCACAT 
CGTGAACCTT CACCTCAGCC TCCACCTCAG CCACCCATGC AGCAATCACT TCCCCAGCAA 
ACTCAGCAGC AAGAACTTCC CTACAGAGAA CAGCACCAGC AATTCTCTCA GCAGTACCTG 
CCAGATGATC TGACACAACA GTTTGATCCA TACAGTGGCC AGCAACAGAT TGGCTATTAC 
CAGCAACCAG AATCACAGAT GGCATTCCAA CAACAACAAC ACCTGCAAGC ATTCCAGCAA 
ATGGGCCAGT TCCCCCAGCA GGCTCAGTAC AACACCCAGC AACAGCAGCA ACACTATACG 
GAACAGTTCG AGCAGCAGCA GCAGCAGCCG CCGCCACCAC CACCTCCACC ATCTGAAGAT 
TTGCGGTCAA GTGCCTACCG CAAGTCTCCT CCCCCGCCGC CACCTCCTGA TACTCCTGCC 
CCACCGAAGG ATGATTCCTT TCAGCAATCT TGGGCAGCTG CACCTCCTCC ACCTCCATCT 
GACTCTTACA CCAGTACCAG ACAATCTGGA GGTCAAGACC AAGACAGATC CAGGGAGAGA 
GGGGATGACA GGCAACGTGG AAGGGGTGAT AAGCAATCTG GAGGTCAAGA CCAAGACAGA 
TCCAGGGAGA GAGGAGATGA CAGGCAACGG GAGTTTCAAT TCAAAACTTA TAAAAATTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_786256.3
CDS	178..6420
Translation	MFNNWNRPGGPGPGGPGERPPMMPGNGMPNRPPFYGPGSGPGAGGRPMHMSGPPGPGPGSHHMGMRMDSMNPRGPMSGPGHWHGSGMNMMMGPGGPGPRRPPPPMSMMHGPGPGGPRPRMGMPIPEPVRVLVNQAQAADTAVPQPPQNEDVKLRGLQDQALHWQATAPVRKEEETVQKQAAAQDQWQSYQQQMQSYQQQYQQWYQSQETTLMDVKLKDLQEKKATCEKQLTDWKNQFDNWVEQHKKHPNKDQFDQYQNQWKTWQTQMESMVNSLDMQIKIKETSLQKNSSKNASTTQTPASVGSGVGTVTTQGANVAMPSGGHGMVPSSGAGMGQGSSIGSIPSSQSVATVPSGSGMGAGPRGLGVGPMPTGSGMGPGPSGTNPRVTPALPQGPGQGPRMESGSRSNSGAGLPGPKGQMPGKDSFPFPFPDDFPFPGDMDNFSFPDMNKLDAMFSGGFKSDEFPFSFPDFPMPGGLMGPDFPLPEGLKPEDMTKDMEEFMKQMESELGNVSAKFQPTPRGSNIADQDKQQALNAKASRGPVDIERGPQGMNRDSAGMGRHPMGMDRGPQGMERHSQEFNRGLSGIDRGPRESLQGRERVPPGMDRGQQGMDRCPPGMDRRRPGMDGGPLGMDRGQPGMDRGQPGMDRGPLGMDRGQPEMDRGPLGMDRGQPGIDGGPLGMNRGQPGMDRGTPGMDRGPPGMDRGPTGMDRGQPSLDRGPPGMDRGQQGMDRGQPGMDRGQPGMDRGQPGMDRGPPGMDRGQQRMDRGQQGMDRGYPGMDRGQTGMDRGPPGMDRGQQGMERVPPGTDKRLPGMDRGPPGMDGGPPRLDRGQPGMDKGPPGMNRGPPGMDGGPPGMDRGHPGMDKGPPGMDRGPPGMERGPPGLDRGHPGMDKGPPGMESGHPGIDKGPPGMDRGTAGRNRGQSEMDRGSSRNEGDAPRTNKVQLGIGGILAGLKDSSGMDNDHRMIDRGPPGIDSGPPGADSGPPGVDSGALRTDRSQLALGNVLAGLRAAPPGMDRGPPGIDGGPPGIDSGPPGIEINAPGRTKSQLGLGSILAALKTGPPGMDTGPPGVEMRPSGMDKGPPGIDIAPPVMDMGPPGTDRGPPSNYIKPPGMDNSPPVMGTLPMSLTRGPPGPPGVDVGPPGVDVGPPGVDIDAISMHSHYLGAGKPRPPFDQRQLDIGPPGVDTFPPGIPRYPFGSTQGPAGFGNSSSIQDSSFEEPMPPGMEPISKPLAFPRDLAPPGMDETFPPGMEPEQESQPSTKSKQPQPPEDNISEFEKGQNKPLFPPPSKGLTQHSYSVGQSIPITGMVKDSFGASSFVSTKQTLAPSPLAGYESLESVKAKKEEKKKDESKSEDKKKGKGKKGNMNMPTIIKFGDSKIDKDSDDPVKVEKDETSTDEKAAKEAAKAKEFKKENSPSEEVSKRVTVRSTLPLITDSTTKSMIPSSIGELFKKNSTTPPAFNKDVKTSLRSYRKAAEDDEEEEEAPHSKKMNRKDSSDRDDRGRDDTLRTTLPSPPRSYRSPDRRSRRSPERRRSRSPDKRKRRSRSRSISQDRRKRRRDSPPRKRNSYSPSKRRSPSKSKNSRSPSRDKGNRSPDRRDQRDRRDQRDQRDQRDRRDGRDDRYREKDRRDRYPDKEYEKDSDRRGGKPSTEAFDKLRELIYDTLVRHPDEKPRDSPHSDRRHPDDRSPQRPRRENYYPEAERPRMRYPPPRRDYERDPLRDRLRSLPRDSPRDLPLPAQREPRRDFDRDSSQDRSMERAHSPPVTEITAPIVFDYGHAESTRPAYDIETRDPMGSLLSNAVIVDVVDYSHGQKQGPDARAISGSELLQERESLFPSEFQIHKEIQPAKRDDTSMQQSPAPPHREPSPQPPPQPPMQQSLPQQTQQQELPYREQHQQFSQQYLPDDLTQQFDPYSGQQQIGYYQQPESQMAFQQQQHLQAFQQMGQFPQQAQYNTQQQQQHYTEQFEQQQQQPPPPPPPPSEDLRSSAYRKSPPPPPPPDTPAPPKDDSFQQSWAAAPPPPPSDSYTSTRQSGGQDQDRSRERGDDRQRGRGDKQSGGQDQDRSRERGDDRQREFQFKTYKNY

Target ORF information:

RefSeq Version	XM_781163.4
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC581149 (LOC581149), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_781163.4

ORF Insert Sequence:

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GATCAGGTAT GAATATGATG ATGGGACCAG GTGGACCAGG ACCAAGAAGA 
CCACCTCCAC CGATGTCGAT GATGCATGGT CCAGGTCCAG GTGGCCCAAG GCCAAGAATG 
GGTATGCCCA TCCCAGAGCC TGTTCGGGTG TTAGTCAATC AAGCGCAAGC CGCAGATACT 
GCAGTCCCAC AACCGCCTCA GAATGAAGAT GTGAAGTTGA GAGGGCTTCA GGATCAGGCC 
TTACATTGGC AGGCAACGGC CCCGGTTCGA AAGGAGGAAG AAACTGTACA AAAACAGGCA 
GCTGCTCAAG ACCAGTGGCA GAGCTACCAG CAACAGATGC AATCATATCA GCAACAGTAT 
CAACAATGGT ACCAAAGTCA AGAGACTACC TTGATGGATG TTAAATTGAA AGACTTGCAG 
GAAAAGAAGG CTACCTGTGA AAAGCAGCTC ACAGACTGGA AAAATCAGTT TGATAATTGG 
GTAGAACAGC ATAAGAAACA TCCAAACAAG GACCAGTTTG ATCAATATCA AAATCAATGG 
AAAACATGGC AAACACAGAT GGAGAGCATG GTGAATTCTT TGGACATGCA AATCAAAATA 
AAAGAGACAT CCCTTCAGAA GAACTCCTCT AAAAATGCTT CAACAACCCA GACACCTGCT 
TCTGTCGGCA GTGGTGTAGG AACAGTTACC ACACAAGGTG CAAATGTAGC AATGCCATCA 
GGTGGGCATG GAATGGTTCC ATCTAGTGGT GCTGGAATGG GACAAGGGTC ATCAATTGGT 
TCTATACCAA GCAGTCAGAG TGTAGCCACA GTTCCAAGTG GTTCTGGGAT GGGAGCAGGA 
CCCAGAGGAC TTGGAGTAGG ACCAATGCCA ACTGGGAGTG GAATGGGACC AGGTCCCTCA 
GGAACAAATC CTAGAGTTAC CCCTGCATTG CCTCAAGGAC CTGGGCAAGG GCCAAGAATG 
GAGAGTGGAA GCAGGTCAAA CTCTGGAGCT GGACTTCCAG GCCCAAAAGG ACAGATGCCT 
GGCAAAGACA GCTTTCCTTT CCCATTTCCT GATGATTTTC CATTTCCAGG AGATATGGAC 
AATTTCTCCT TCCCTGATAT GAATAAACTA GATGCAATGT TTTCAGGTGG TTTTAAATCT 
GATGAGTTTC CATTTTCCTT CCCTGATTTT CCGATGCCAG GTGGTTTAAT GGGACCCGAC 
TTTCCATTAC CTGAAGGACT GAAACCCGAA GACATGACCA AAGATATGGA AGAATTCATG 
AAACAGATGG AATCTGAACT AGGCAATGTT TCTGCAAAAT TTCAACCAAC CCCAAGGGGA 
AGCAACATAG CCGATCAGGA TAAACAGCAA GCTTTAAATG CCAAAGCATC AAGAGGACCT 
GTAGACATAG AGAGGGGTCC ACAAGGAATG AACAGAGATT CTGCTGGGAT GGGAAGACAC 
CCAATGGGAA TGGATAGAGG TCCTCAAGGT ATGGAGAGAC ACTCACAAGA ATTCAATAGA 
GGTCTATCAG GAATAGACAG AGGCCCAAGA GAAAGTCTTC AAGGGAGGGA AAGGGTCCCA 
CCAGGAATGG ATAGAGGACA ACAAGGAATG GATAGATGCC CTCCCGGAAT GGATAGAAGA 
AGACCAGGCA TGGACGGAGG CCCTCTTGGA ATGGATAGAG GACAACCAGG AATGGATAGA 
GGACAACCAG GAATGGACAG AGGCCCTCTT GGAATGGATA GAGGACAACC AGAAATGGAC 
AGAGGCCCTC TTGGAATGGA TAGAGGACAA CCAGGAATTG ACGGAGGCCC TCTTGGAATG 
AATAGAGGAC AACCAGGAAT GGATAGAGGC ACTCCTGGAA TGGATAGAGG TCCCCCTGGG 
ATGGATAGGG GACCAACCGG AATGGATAGA GGACAACCAA GCTTGGATAG AGGTCCTCCT 
GGAATGGATA GAGGACAGCA AGGAATGGAT AGAGGTCAAC CAGGAATGGA TAGAGGACAA 
CCAGGAATGG ATAGAGGACA ACCAGGAATG GATAGAGGTC CTCCTGGAAT GGATAGAGGA 
CAGCAACGAA TGGATAGAGG ACAGCAAGGA ATGGATAGAG GTTATCCTGG AATGGATAGA 
GGACAAACAG GAATGGATAG AGGTCCTCCT GGAATGGATA GAGGACAGCA AGGAATGGAG 
AGAGTTCCTC CTGGAACAGA CAAAAGACTA CCCGGAATGG ATAGAGGCCC TCCTGGAATG 
GATGGAGGCC CTCCCAGACT GGATAGAGGA CAACCAGGCA TGGATAAAGG CCCTCCTGGA 
ATGAATAGAG GCCCTCCCGG AATGGATGGA GGCCCTCCCG GAATGGATAG AGGACATCCA 
GGCATGGATA AAGGCCCTCC TGGAATGGAT AGAGGCCCTC CCGGAATGGA GAGAGGCCCA 
CCTGGACTGG ATAGAGGACA TCCAGGCATG GATAAAGGCC CTCCTGGAAT GGAGAGTGGA 
CATCCAGGCA TAGATAAAGG CCCTCCTGGA ATGGATAGAG GTACAGCAGG AAGGAATAGA 
GGCCAATCAG AAATGGATAG AGGCTCATCA AGAAATGAGG GAGATGCACC AAGAACTAAT 
AAAGTTCAAC TAGGGATCGG GGGAATTCTT GCAGGACTTA AAGATTCATC AGGTATGGAT 
AATGATCATA GAATGATTGA CAGAGGCCCA CCAGGAATTG ACAGTGGCCC TCCAGGAGCA 
GACAGTGGCC CACCAGGAGT AGACAGTGGT GCATTGAGAA CCGATAGAAG TCAACTAGCT 
TTAGGGAACG TTCTTGCTGG GTTGAGGGCA GCCCCACCAG GAATGGATAG AGGCCCACCA 
GGAATAGACG GTGGCCCACC AGGAATAGAC AGTGGCCCAC CAGGAATAGA AATAAACGCC 
CCAGGTCGTA CTAAAAGTCA ACTAGGTTTA GGGAGCATTC TTGCTGCACT GAAGACAGGT 
CCACCAGGAA TGGATACGGG TCCACCTGGT GTGGAGATGA GACCTAGTGG CATGGACAAG 
GGACCCCCGG GAATAGACAT AGCCCCTCCA GTTATGGATA TGGGTCCTCC AGGAACGGAC 
AGAGGACCTC CATCTAATTA CATTAAACCT CCAGGGATGG ACAACTCACC TCCAGTTATG 
GGGACATTGC CCATGAGTTT GACCAGGGGT CCTCCTGGTC CACCTGGAGT TGATGTTGGA 
CCTCCTGGTG TCGATGTTGG ACCTCCTGGT GTGGATATTG ATGCAATTAG TATGCATAGC 
CATTATTTGG GAGCTGGAAA GCCTCGCCCT CCATTTGATC AAAGGCAATT GGATATTGGT 
CCACCAGGTG TAGATACCTT TCCCCCTGGA ATTCCACGTT ATCCTTTTGG TTCAACACAA 
GGTCCAGCAG GATTTGGAAA CTCATCTTCA ATTCAAGATA GCAGCTTTGA AGAGCCAATG 
CCTCCTGGTA TGGAGCCTAT TAGTAAACCC CTTGCTTTTC CCAGAGATCT TGCACCTCCT 
GGGATGGATG AGACATTTCC ACCAGGGATG GAACCAGAAC AGGAAAGCCA GCCAAGCACA 
AAAAGTAAAC AGCCGCAACC ACCGGAAGAC AATATAAGTG AATTTGAAAA GGGGCAAAAC 
AAGCCACTGT TTCCCCCACC ATCCAAAGGT TTGACTCAAC ATTCCTATTC AGTTGGACAA 
TCCATACCAA TTACAGGTAT GGTGAAAGAT TCTTTTGGTG CCAGCTCCTT TGTTTCAACA 
AAACAGACAT TAGCTCCAAG CCCACTTGCT GGGTATGAGT CATTAGAATC GGTAAAGGCC 
AAGAAAGAAG AAAAGAAAAA AGATGAATCA AAGTCAGAGG ATAAAAAAAA AGGAAAAGGG 
AAAAAGGGAA ATATGAACAT GCCTACAATC ATCAAATTTG GGGATTCTAA AATTGATAAA 
GATTCTGATG ATCCTGTCAA AGTTGAAAAG GACGAGACAT CTACTGATGA AAAAGCAGCA 
AAGGAGGCTG CAAAAGCTAA GGAGTTTAAA AAAGAAAATT CACCTTCTGA GGAGGTTTCA 
AAGAGAGTAA CTGTGCGTAG CACCCTACCA TTGATCACAG ATTCTACAAC CAAGTCTATG 
ATACCTTCAT CAATTGGTGA GCTATTCAAG AAGAACTCTA CAACACCTCC AGCATTCAAC 
AAAGATGTAA AGACCTCACT TAGGAGCTAC AGGAAAGCAG CAGAAGATGA TGAGGAAGAG 
GAAGAAGCGC CTCACTCTAA AAAGATGAAT AGGAAGGATT CTAGCGATAG AGATGATAGG 
GGACGGGATG ACACACTCCG CACTACACTC CCATCCCCCC CACGGTCATA TCGGTCACCT 
GATCGAAGGT CTAGGAGATC TCCTGAAAGA AGGAGATCTC GTTCTCCAGA CAAAAGAAAG 
CGTAGATCTC GGTCTAGATC GATCTCACAG GACCGCAGGA AACGGAGGCG GGATTCTCCA 
CCCCGTAAGA GAAACTCATA CTCTCCGAGC AAAAGGAGGT CCCCCTCTAA AAGTAAAAAT 
TCACGATCCC CAAGCAGAGA CAAGGGCAAT AGATCCCCGG ATCGAAGAGA TCAGAGAGAT 
CGAAGAGATC AGAGAGATCA AAGAGATCAG AGAGATCGAA GAGATGGGAG AGATGACAGA 
TACAGAGAAA AGGACAGGAG GGACAGGTAT CCTGACAAAG AATATGAGAA AGATTCTGAT 
AGGAGAGGTG GGAAGCCAAG TACAGAAGCC TTTGACAAGT TGCGAGAGCT GATTTATGAT 
ACCTTGGTGC GCCATCCTGA TGAAAAGCCA AGGGATTCTC CACATAGCGA TAGGCGACAC 
CCGGACGATA GATCTCCTCA GCGACCAAGA AGGGAGAACT ATTACCCGGA AGCTGAGAGG 
CCAAGAATGC GATATCCTCC ACCACGTAGA GACTATGAAA GAGATCCTTT GAGGGATCGG 
TTAAGAAGCC TTCCAAGGGA TTCACCAAGG GATCTTCCAT TGCCAGCTCA AAGGGAGCCT 
CGTAGGGATT TTGATAGAGA TTCCTCTCAA GACAGATCTA TGGAGAGGGC TCATTCCCCT 
CCGGTGACGG AAATAACAGC ACCTATTGTC TTTGACTATG GGCATGCAGA GTCCACCAGG 
CCTGCTTATG ACATAGAGAC AAGAGATCCC ATGGGAAGCC TGCTCAGCAA TGCAGTTATT 
GTAGACGTTG TTGATTATAG TCATGGTCAG AAGCAGGGAC CAGATGCTAG AGCAATTAGC 
GGATCAGAAC TTCTTCAAGA GCGGGAGTCA TTGTTCCCTT CAGAATTTCA AATACATAAA 
GAGATTCAAC CCGCAAAGAG AGATGATACC AGTATGCAAC AATCACCAGC TCCACCACAT 
CGTGAACCTT CACCTCAGCC TCCACCTCAG CCACCCATGC AGCAATCACT TCCCCAGCAA 
ACTCAGCAGC AAGAACTTCC CTACAGAGAA CAGCACCAGC AATTCTCTCA GCAGTACCTG 
CCAGATGATC TGACACAACA GTTTGATCCA TACAGTGGCC AGCAACAGAT TGGCTATTAC 
CAGCAACCAG AATCACAGAT GGCATTCCAA CAACAACAAC ACCTGCAAGC ATTCCAGCAA 
ATGGGCCAGT TCCCCCAGCA GGCTCAGTAC AACACCCAGC AACAGCAGCA ACACTATACG 
GAACAGTTCG AGCAGCAGCA GCAGCAGCCG CCGCCACCAC CACCTCCACC ATCTGAAGAT 
TTGCGGTCAA GTGCCTACCG CAAGTCTCCT CCCCCGCCGC CACCTCCTGA TACTCCTGCC 
CCACCGAAGG ATGATTCCTT TCAGCAATCT TGGGCAGCTG CACCTCCTCC ACCTCCATCT 
GACTCTTACA CCAGTACCAG ACAATCTGGA GGTCAAGACC AAGACAGATC CAGGGAGAGA 
GGGGATGACA GGCAACGTGG AAGGGGTGAT AAGCAATCTG GAGGTCAAGA CCAAGACAGA 
TCCAGGGAGA GAGGAGATGA CAGGCAACGG GAGTTTCAAT TCAAAACTTA TAAAAATTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC581149 ( XM_030999828.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030855688.1 Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X1, mRNA.

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CloneID	OSd65788
Clone ID Related Accession (Same CDS sequence)	XM_030999828.1
Accession Version	XM_030999828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	YLP motif-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GACCAGGTAT GAATATGATG GGACCAGGTG GACCAGGACC AAGAAGACCA 
CCTCCACCGA TGACGATGTC GATGATGCAT GGTCCTGGTC CAGGTGGCCC AAGGCCAAGA 
ATGGGTATGC CCATCCCAGA GCCTGTTCGG GTGTTAGTCA ATCAAGCGCA AGCCGCAGAT 
ACTGCAGTCC CACAACCGCC TCAGAATGAA GATGTGAAGT TGAGAGGGCT TCAGGATCAG 
GCCTTACATT GGCAGGCAAC GGCCCCGGTT CGAAAGGAGG AAGAAACTGT ACAAAAACAG 
GCAGCTGCTC AAGACCAGTG GCAGAGCTAC CAGCAACAGA TGCAATCATA TCAGCAACAG 
TATCAACAAT GGTACCAAAG TCAAGAGACT ACCTTGATGG ATGTTAAATT GAAAGACTTG 
CAGGAAAAGA AGGCTACCTG TGAAAAGCAG CTCACAGACT GGAAAAATCA GTTTGATAAT 
TGGGTAGAAC AGCATAAGAA ACATCCAAAC AAGGACCAGT TTGATCAATA TCAAAATCAA 
TGGAAAACAT GGCAAACACA GATGGAGAGC ATGGTGAATT CTTTGGACAT GCAAATCAAA 
ATAAAAGAGA CATCCCTTCA GAAGAACTCC TCTAAAAATG CTTCAACAAC CCAGACACCT 
GCTTCTGTCG GCAGTGGTGT AGGAACAGTT ACCACACAAG GTGCAAATGT AGCAATGCCA 
TCAGGTGGGC ATGGAATGGT TCCATCTAGT GGTGCTGGAA TGGGACAAGG GTCATCAATT 
GGTTCTATAC CAAGCAGTCA GAGTGTAGCC ACAGTTCCAA GTGGTTCTGG GATGGGAGCA 
GGACCCAGAG GACTTGGAGT AGGACCAATG CCAACTGGGA GTGGAATGGG ACCAGGTCCC 
TCAGGAACAA ATCCTAGAGT TACCCCTGCA TTGCCTCAAG GACCTGGGCA AGGGCCAAGA 
ATGGAGAGTG GAAGCAGGTC AAACTCTGGA GCTGGACTTC CAGGCCCAAA AGGACAGATG 
CCTGGCAAAG ACAGCTTTCC TTTCCCATTT CCTGATGATT TTCCATTTCC AGGAGATATG 
GACAATTTCT CCTTCCCTGA TATGAATAAA CTAGATGCAA TGTTTTCAGG TGGTTTTAAA 
TCTGATGAGT TTCCATTTTC CTTCCCTGAT TTTCCGATGC CAGGTGGTTT AATGGGACCC 
GACTTTCCAT TACCTGAAGG ACTGAAACCC GAAGACATGA CCAAAGATAT GGAAGAATTC 
ATGAAACAGA TGGAATCTGA ACTAGGCAAT GTTTCTGCAA AATTTCAACC AACCCCAAGG 
GGAAGCAACA TAGCCGATCA GGATAAACAG CAAGCTTTAA ATGCCAAAGC ATCAAGAGGA 
CCTGTAGACA TAGAGAGGGG TCCACAAGGA ATGAACAGAG ATTCTGCTGG GATGGGAAGA 
CACCCAATGG GAATGGATAG AGGTCCTCAA GGTATGGAGA GACACTCACA AGAATTCAAT 
AGAGGTCTAT CAGGAATAGA CAGAGGCCCA AGAGAAAGTC TTCAAGGGAG GGAAAGGGTC 
CCACCAGGAA TGGATAGAGG ACAACAAGGA ATGGATAGAT GCCCTCCCGG AATGGATAGA 
AGAAGACCAG GCATGGACGG AGGCCCTCTT GGAATGGATA GAGGACAACC AGGAATGGAT 
AGAGGACAAC CAGGAATGGA CAGAGGCCCT CTTGGAATGG ATAGAGGACA ACCAGAAATG 
GACAGAGGCC CTCTTGGAAT GGATAGAGGA CAACCAGGAA TTGACGGAGG CCCTCTTGGA 
ATGAATAGAG GACAACCAGG AATGGATAGA GGCACTCCTG GAATGGATAG AGGTCCCCCT 
GGGATGGATA GGGGACCAAC CGGAATGGAT AGAGGACAAC CAAGCTTGGA TAGAGGTCCT 
CCTGGAATGG ATAGAGGACA GCAAGGAATG GATAGAGGTC AACCAGGAAT GGATAGAGGA 
CAACCAGGAA TGGATAGAGG ACAACCAGGA ATGGATAGAG GTCCTCCTGG AATGGATAGA 
GGACAGCAAC GAATGGATAG AGGACAGCAA GGAATGGATA GAGGTTATCC TGGAATGGAT 
AGAGGACAAA CAGGAATGGA TAGAGGTCCT CCTGGAATGG ATAGAGGACA GCAAGGAATG 
GAGAGAGTTC CTCCTGGAAC AGACAAAAGA CTACCCGGAA TGGATAGAGG CCCTCCTGGA 
ATGGATGGAG GCCCTCCCAG ACTGGATAGA GGACAACCAG GCATGGATAA AGGCCCTCCT 
GGAATGAATA GAGGCCCTCC CGGAATGGAT GGAGGCCCTC CCGGAATGGA TAGAGGACAT 
CCAGGCATGG ATAAAGGCCC TCCTGGAATG GATAGAGGCC CTCCCGGAAT GGAGAGAGGC 
CCACCTGGAC TGGATAGAGG ACATCCAGGC ATGGATAAAG GCCCTCCTGG AATGGAGAGT 
GGACATCCAG GCATAGATAA AGGCCCTCCT GGAATGGATA GAGGTACAGC AGGAAGGAAT 
AGAGGCCAAT CAGAAATGGA TAGAGGCTCA TCAAGAAATG AGGGAGATGC ACCAAGAACT 
AATAAAGTTC AACTAGGGAT CGGGGGAATT CTTGCAGGAC TTAAAGATTC ATCAGGTATG 
GATAATGATC ATAGAATGAT TGACAGAGGC CCACCAGGAA TTGACAGTGG CCCTCCAGGA 
GCAGACAGTG GCCCACCAGG AGTAGACAGT GGTGCATTGA GAACCGATAG AAGTCAACTA 
GCTTTAGGGA ACGTTCTTGC TGGGTTGAGG GCAGCCCCAC CAGGAATGGA TAGAGGCCCA 
CCAGGAATAG ACGGTGGCCC ACCAGGAATA GACAGTGGCC CACCAGGAAT AGAAATAAAC 
GCCCCAGGTC GTACTAAAAG TCAACTAGGT TTAGGGAGCA TTCTTGCTGC ACTGAAGACA 
GGTCCACCAG GAATGGATAC GGGTCCACCT GGTGTGGAGA TGAGACCTAG TGGCATGGAC 
AAGGGACCCC CGGGAATAGA CATAGCCCCT CCAGTTATGG ATATGGGTCC TCCAGGAACG 
GACAGAGGAC CTCCATCTAA TTACATTAAA CCTCCAGGGA TGGACAACTC ACCTCCAGTT 
ATGGGGACAT TGCCCATGAG TTTGACCAGG GGTCCTCCTG GTCCACCTGG AGTTGATGTT 
GGACCTCCTG GTGTCGATGT TGGACCTCCT GGTGTGGATA TTGATGCAAT TAGTATGCAT 
AGCCATTATT TGGGAGCTGG AAAGCCTCGC CCTCCATTTG ATCAAAGGCA ATTGGATATT 
GGTCCACCAG GTGTAGATAC CTTTCCCCCT GGAATTCCAC GTTATCCTTT TGGTTCAACA 
CAAGGTCCAG CAGGATTTGG AAACTCATCT TCAATTCAAG ATAGCAGCTT TGAAGAGCCA 
ATGCCTCCTG GTATGGAGCC TATTAGTAAA CCCCTTGCTT TTCCCAGAGA TCTTGCACCT 
CCTGGGATGG ATGAGACATT TCCACCAGGG ATGGAACCAG AACAGGAAAG CCAGCCAAGC 
ACAAAAAGTA AACAGCCGCA ACCACCGGAA GACAATATAA GTGAATTTGA AAAGGGGCAA 
AACAAGCCAC TGTTTCCCCC ACCATCCAAA GGTTTGACTC AACATTCCTA TTCAGTTGGA 
CAATCCATAC CAATTACAGG TATGGTGAAA GATTCTTTTG GTGCCAGCTC CTTTGTTTCA 
ACAAAACAGA CATTAGCTCC AAGCCCACTT GCTGGGTATG AGTCATTAGA ATCGGTAAAG 
GCCAAGAAAG AAGAAAAGAA AAAAGATGAA TCAAAGTCAG AGGATAAAAA AAAAGGAAAA 
GGGAAAAAGG GAAATATGAA CATGCCTACA ATCATCAAAT TTGGGGATTC TAAAATTGAT 
AAAGATTCTG ATGATCCTGT CAAAGTTGAA AAGGACGAGA CATCTACTGA TGAAAAAGCA 
GCAAAGGAGG CTGCAAAAGC TAAGGAGTTT AAAAAAGAAA ATTCACCTTC TGAGGAGGTT 
TCAAAGAGAG TAACTGTGCG TAGCACCCTA CCATTGATCA CAGATTCTAC AACCAAGTCT 
ATGATACCTT CATCAATTGG TGAGCTATTC AAGAAGAACT CTACAACACC TCCAGCATTC 
AACAAAGATG TAAAGACCTC ACTTAGGAGC TACAGGAAAG CAGCAGAAGA TGATGAGGAA 
GAGGAAGAAG CGCCTCACTC TAAAAAGATG AATAGGAAGG ATTCTAGCGA TAGAGATGAT 
AGGGGACGGG ATGACACACT CCGCACTACA CTCCCATCCC CCCCACGGTC ATATCGGTCA 
CCTGATCGAA GGTCTAGGAG ATCTCCTGAA AGAAGGAGAT CTCGTTCTCC AGACAAAAGA 
AAGCGTAGAT CTCGGTCTAG ATCGATCTCA CAGGACCGCA GGAAACGGAG GCGGGATTCT 
CCACCCCGTA AGAGAAACTC ATACTCTCCG AGCAAAAGGA GGTCCCCCTC TAAAAGTAAA 
AATTCACGAT CCCCAAGCAG AGACAAGGGC AATAGATCCC CGGATCGAAG AGATCAGAGA 
GATCGAAGAG ATCAGAGAGA TCAAAGAGAT CAGAGAGATC GAAGAGATGG GAGAGATGAC 
AGATACAGAG AAAAGGACAG GAGGGACAGG TATCCTGACA AAGAATATGA GAAAGATTCT 
GATAGGAGAG GTGGGAAGCC AAGTACAGAA GCCTTTGACA AGTTGCGAGA GCTGATTTAT 
GATACCTTGG TGCGCCATCC TGATGAAAAG CCAAGGGATT CTCCACATAG CGATAGGCGA 
CACCCGGACG ATAGATCTCC TCAGCGACCA AGAAGGGAGA ACTATTACCC GGAAGCTGAG 
AGGCCAAGAA TGCGATACCC TCCACCACGT AGAGACTATG AAAGAGATCC TTTGAGGGAT 
CGGTTAAGAA GCCTTCCAAG GGATTCACCA AGGGATCTTC CATTGCCAGC TCAAAGGGAG 
CCTCGTAGGG ATTTTGATAG AGATTCCTCT CAAGACAGAT CTATGGAGAG GGCTCATTCC 
CCTCCGGTGA CGGAAATAAC AGCACCTATT GTCTTTGACT ATGGGCATGC AGAGTCCACC 
AGGCCTGCTT ATGACATAGA GACAAGAGAT CCCATGGGAA GCCTGCTCAG CAATGCAGTT 
ATTGTAGACG TTGTTGATTA TAGTCATGGT CAGAATCAGG GACCAGATGC TAGAGCAATT 
AGCGGATCAG AACTTCTTCA AGAGCGGGAG TCATCGTTCC CTTCAGAATT TCAAATACAT 
AAAGAGATTC AACCCGCAAA GAGAGATGAT ACCAGTATGC AACAATCACC AGCTCCACCA 
CATCGTGAAC CTTCACCTCA GCCTCCACCT CAGCCACCCA TGCAGCAATC ACTTCCCCAG 
CAAACTCAGC AGCAAGAACT TCCCTACAGA GAACAGCACC AGCAATTCTC TCAGCAGTAC 
CTGCCAGATG ATCTGACACA ACAGTTTGAT CCATACAGTG GCCAGCAACA GATTGGCTAT 
TACCAGCAAC CAGAATCACA GATGGCATTC CAACAACAAC AACACCTGCA AGCATTCCAG 
CAAATGGGCC AGTTCCCCCA GCAGGCTCAG TACAACACCC AGCAACAGCA GCAACACTAT 
ACGGAACAGT TCGAGCAGCA GCAGCAGCAG CCGCCGCCAC CACCACCTCC ACCATCTGAA 
GATTTGCGGT CAAGTGCCTA CCGCAAGTCC CCTCCCCCGC CGCCACCTGA TACTCCTGCC 
CCACCGAAGG ATGATTCCTT TCAGCAATCT TGGGCAGCTG CACCTCCCCC ACCTCCATCT 
GACTCTTACA CCAGTACCAG ACAATCTGGA GGTCAAGACC AAGACAGATC CAGGGAGAGA 
GGGGATGACA GGCAACGTGG AAGGGGTGAT AAGCAATCTG GAGGTCAAGA CCAAGACAGA 
TCCAGGGAGA GAGGAGATGA CAGGCAACGT GGAAGGGGGG ATAAATACCA GAGTGGCCCT 
GCCAGGCTAG TGGAGGACTA CAGGGACTCC CCTGGACAGA AGGACATGTT CACCAACTCA 
CCCCAGCAAA CCCCTCCTCC CCCTTCCAAG CCCAAGAAGA GGGAAGTGGT GGCAGTCACG 
GACCTCCTAG ACCCGCCCAA CAGGGAGAAA CGCCCAGCCA AGATCTGTAT GATCCTGCGT 
GGCCCTCCTG GAAGCGGCAA GAGTTACATT GCAAAGCTTA TCAAGGACAG AGAAATACAA 
AACCGAGGTC CTAGTCCTCG CATGCTGTGT CTGGATGACT ACTTTATGAG TGAGAAGGAG 
AAGACGGAAA AGGACGCTGA AACTGGCAAG ATTGTCAAGA AGAGGTTTCA GGAATACGAA 
CATGAGGACA GCATGGAGGC TATTTACCAT GTCAGTTTAC TCAAGACATT CAAGAAGACC 
ATCGAAGACG GTTTCTTTGA CTTCATCATC GTGGATGCCG TCAACGACAA AATCTCAAAG 
ATTGAACCGT TCTGGAGCTA TGCAAAGATG AAAGGATTTG AGGTGTATGT AGCTGAGTTA 
CTTGTTGAAT TAGGCTTGTG CATTCAGCGA GACATCCACA ATAGAGGTGA AACAGGAATC 
AGAAAGATCA TAGAACACTG GGAGTTTGCC CCACGGAAAT ACATCAAGCT AGATGTGGTG 
GCCCTCCTGC AAGATGCTGC CATTCCAGAG GTTGAGATGG AGGAACTTGA TGAAGACGCC 
GGACCTTCCA TGGAGGAACT GATGAACCAA CAGCAGGAAG CCTTGGAAGA ACTAGAGGAT 
CAGGAAGAGA CACTGGCTTT GACGAAGAGT AAGTGGGATT CTGCTACCGA AGAGAAACTA 
GATGCCCTTG ATGGTATCAA GAAGTATAAG AAGAAGGAGA GCTACACGTC TATGGAGGAC 
TTCCTATCAT GTGGAGATGA GTTTGACACA GTCTCCAAAC CCATACTCCC AGGACAGAAG 
AGAGTACGCT GGGCCGACAT CGAGGAACGC CAAAACCAAG AACGTATGCG TGCAGTAGGT 
TTTGTCGTTG GGCAGACGGC CACTGACTGG GCCAAGATCA CTGACGACAA TTATGCAGAC 
AAGGCTCTCA ATCGCACCAA GTACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030855688.1
CDS	124..7473
Translation	MFNNWNRPGGPGPGGPGERPPMMPGNGMPNRPPFYGPGSGPGAGGRPMHMSGPPGPGPGSHHMGMRMDSMNPRGPMSGPGHWHGPGMNMMGPGGPGPRRPPPPMTMSMMHGPGPGGPRPRMGMPIPEPVRVLVNQAQAADTAVPQPPQNEDVKLRGLQDQALHWQATAPVRKEEETVQKQAAAQDQWQSYQQQMQSYQQQYQQWYQSQETTLMDVKLKDLQEKKATCEKQLTDWKNQFDNWVEQHKKHPNKDQFDQYQNQWKTWQTQMESMVNSLDMQIKIKETSLQKNSSKNASTTQTPASVGSGVGTVTTQGANVAMPSGGHGMVPSSGAGMGQGSSIGSIPSSQSVATVPSGSGMGAGPRGLGVGPMPTGSGMGPGPSGTNPRVTPALPQGPGQGPRMESGSRSNSGAGLPGPKGQMPGKDSFPFPFPDDFPFPGDMDNFSFPDMNKLDAMFSGGFKSDEFPFSFPDFPMPGGLMGPDFPLPEGLKPEDMTKDMEEFMKQMESELGNVSAKFQPTPRGSNIADQDKQQALNAKASRGPVDIERGPQGMNRDSAGMGRHPMGMDRGPQGMERHSQEFNRGLSGIDRGPRESLQGRERVPPGMDRGQQGMDRCPPGMDRRRPGMDGGPLGMDRGQPGMDRGQPGMDRGPLGMDRGQPEMDRGPLGMDRGQPGIDGGPLGMNRGQPGMDRGTPGMDRGPPGMDRGPTGMDRGQPSLDRGPPGMDRGQQGMDRGQPGMDRGQPGMDRGQPGMDRGPPGMDRGQQRMDRGQQGMDRGYPGMDRGQTGMDRGPPGMDRGQQGMERVPPGTDKRLPGMDRGPPGMDGGPPRLDRGQPGMDKGPPGMNRGPPGMDGGPPGMDRGHPGMDKGPPGMDRGPPGMERGPPGLDRGHPGMDKGPPGMESGHPGIDKGPPGMDRGTAGRNRGQSEMDRGSSRNEGDAPRTNKVQLGIGGILAGLKDSSGMDNDHRMIDRGPPGIDSGPPGADSGPPGVDSGALRTDRSQLALGNVLAGLRAAPPGMDRGPPGIDGGPPGIDSGPPGIEINAPGRTKSQLGLGSILAALKTGPPGMDTGPPGVEMRPSGMDKGPPGIDIAPPVMDMGPPGTDRGPPSNYIKPPGMDNSPPVMGTLPMSLTRGPPGPPGVDVGPPGVDVGPPGVDIDAISMHSHYLGAGKPRPPFDQRQLDIGPPGVDTFPPGIPRYPFGSTQGPAGFGNSSSIQDSSFEEPMPPGMEPISKPLAFPRDLAPPGMDETFPPGMEPEQESQPSTKSKQPQPPEDNISEFEKGQNKPLFPPPSKGLTQHSYSVGQSIPITGMVKDSFGASSFVSTKQTLAPSPLAGYESLESVKAKKEEKKKDESKSEDKKKGKGKKGNMNMPTIIKFGDSKIDKDSDDPVKVEKDETSTDEKAAKEAAKAKEFKKENSPSEEVSKRVTVRSTLPLITDSTTKSMIPSSIGELFKKNSTTPPAFNKDVKTSLRSYRKAAEDDEEEEEAPHSKKMNRKDSSDRDDRGRDDTLRTTLPSPPRSYRSPDRRSRRSPERRRSRSPDKRKRRSRSRSISQDRRKRRRDSPPRKRNSYSPSKRRSPSKSKNSRSPSRDKGNRSPDRRDQRDRRDQRDQRDQRDRRDGRDDRYREKDRRDRYPDKEYEKDSDRRGGKPSTEAFDKLRELIYDTLVRHPDEKPRDSPHSDRRHPDDRSPQRPRRENYYPEAERPRMRYPPPRRDYERDPLRDRLRSLPRDSPRDLPLPAQREPRRDFDRDSSQDRSMERAHSPPVTEITAPIVFDYGHAESTRPAYDIETRDPMGSLLSNAVIVDVVDYSHGQNQGPDARAISGSELLQERESSFPSEFQIHKEIQPAKRDDTSMQQSPAPPHREPSPQPPPQPPMQQSLPQQTQQQELPYREQHQQFSQQYLPDDLTQQFDPYSGQQQIGYYQQPESQMAFQQQQHLQAFQQMGQFPQQAQYNTQQQQQHYTEQFEQQQQQPPPPPPPPSEDLRSSAYRKSPPPPPPDTPAPPKDDSFQQSWAAAPPPPPSDSYTSTRQSGGQDQDRSRERGDDRQRGRGDKQSGGQDQDRSRERGDDRQRGRGDKYQSGPARLVEDYRDSPGQKDMFTNSPQQTPPPPSKPKKREVVAVTDLLDPPNREKRPAKICMILRGPPGSGKSYIAKLIKDREIQNRGPSPRMLCLDDYFMSEKEKTEKDAETGKIVKKRFQEYEHEDSMEAIYHVSLLKTFKKTIEDGFFDFIIVDAVNDKISKIEPFWSYAKMKGFEVYVAELLVELGLCIQRDIHNRGETGIRKIIEHWEFAPRKYIKLDVVALLQDAAIPEVEMEELDEDAGPSMEELMNQQQEALEELEDQEETLALTKSKWDSATEEKLDALDGIKKYKKKESYTSMEDFLSCGDEFDTVSKPILPGQKRVRWADIEERQNQERMRAVGFVVGQTATDWAKITDDNYADKALNRTKYI

Target ORF information:

RefSeq Version	XM_030999828.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030999828.1

ORF Insert Sequence:

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GACCAGGTAT GAATATGATG GGACCAGGTG GACCAGGACC AAGAAGACCA 
CCTCCACCGA TGACGATGTC GATGATGCAT GGTCCTGGTC CAGGTGGCCC AAGGCCAAGA 
ATGGGTATGC CCATCCCAGA GCCTGTTCGG GTGTTAGTCA ATCAAGCGCA AGCCGCAGAT 
ACTGCAGTCC CACAACCGCC TCAGAATGAA GATGTGAAGT TGAGAGGGCT TCAGGATCAG 
GCCTTACATT GGCAGGCAAC GGCCCCGGTT CGAAAGGAGG AAGAAACTGT ACAAAAACAG 
GCAGCTGCTC AAGACCAGTG GCAGAGCTAC CAGCAACAGA TGCAATCATA TCAGCAACAG 
TATCAACAAT GGTACCAAAG TCAAGAGACT ACCTTGATGG ATGTTAAATT GAAAGACTTG 
CAGGAAAAGA AGGCTACCTG TGAAAAGCAG CTCACAGACT GGAAAAATCA GTTTGATAAT 
TGGGTAGAAC AGCATAAGAA ACATCCAAAC AAGGACCAGT TTGATCAATA TCAAAATCAA 
TGGAAAACAT GGCAAACACA GATGGAGAGC ATGGTGAATT CTTTGGACAT GCAAATCAAA 
ATAAAAGAGA CATCCCTTCA GAAGAACTCC TCTAAAAATG CTTCAACAAC CCAGACACCT 
GCTTCTGTCG GCAGTGGTGT AGGAACAGTT ACCACACAAG GTGCAAATGT AGCAATGCCA 
TCAGGTGGGC ATGGAATGGT TCCATCTAGT GGTGCTGGAA TGGGACAAGG GTCATCAATT 
GGTTCTATAC CAAGCAGTCA GAGTGTAGCC ACAGTTCCAA GTGGTTCTGG GATGGGAGCA 
GGACCCAGAG GACTTGGAGT AGGACCAATG CCAACTGGGA GTGGAATGGG ACCAGGTCCC 
TCAGGAACAA ATCCTAGAGT TACCCCTGCA TTGCCTCAAG GACCTGGGCA AGGGCCAAGA 
ATGGAGAGTG GAAGCAGGTC AAACTCTGGA GCTGGACTTC CAGGCCCAAA AGGACAGATG 
CCTGGCAAAG ACAGCTTTCC TTTCCCATTT CCTGATGATT TTCCATTTCC AGGAGATATG 
GACAATTTCT CCTTCCCTGA TATGAATAAA CTAGATGCAA TGTTTTCAGG TGGTTTTAAA 
TCTGATGAGT TTCCATTTTC CTTCCCTGAT TTTCCGATGC CAGGTGGTTT AATGGGACCC 
GACTTTCCAT TACCTGAAGG ACTGAAACCC GAAGACATGA CCAAAGATAT GGAAGAATTC 
ATGAAACAGA TGGAATCTGA ACTAGGCAAT GTTTCTGCAA AATTTCAACC AACCCCAAGG 
GGAAGCAACA TAGCCGATCA GGATAAACAG CAAGCTTTAA ATGCCAAAGC ATCAAGAGGA 
CCTGTAGACA TAGAGAGGGG TCCACAAGGA ATGAACAGAG ATTCTGCTGG GATGGGAAGA 
CACCCAATGG GAATGGATAG AGGTCCTCAA GGTATGGAGA GACACTCACA AGAATTCAAT 
AGAGGTCTAT CAGGAATAGA CAGAGGCCCA AGAGAAAGTC TTCAAGGGAG GGAAAGGGTC 
CCACCAGGAA TGGATAGAGG ACAACAAGGA ATGGATAGAT GCCCTCCCGG AATGGATAGA 
AGAAGACCAG GCATGGACGG AGGCCCTCTT GGAATGGATA GAGGACAACC AGGAATGGAT 
AGAGGACAAC CAGGAATGGA CAGAGGCCCT CTTGGAATGG ATAGAGGACA ACCAGAAATG 
GACAGAGGCC CTCTTGGAAT GGATAGAGGA CAACCAGGAA TTGACGGAGG CCCTCTTGGA 
ATGAATAGAG GACAACCAGG AATGGATAGA GGCACTCCTG GAATGGATAG AGGTCCCCCT 
GGGATGGATA GGGGACCAAC CGGAATGGAT AGAGGACAAC CAAGCTTGGA TAGAGGTCCT 
CCTGGAATGG ATAGAGGACA GCAAGGAATG GATAGAGGTC AACCAGGAAT GGATAGAGGA 
CAACCAGGAA TGGATAGAGG ACAACCAGGA ATGGATAGAG GTCCTCCTGG AATGGATAGA 
GGACAGCAAC GAATGGATAG AGGACAGCAA GGAATGGATA GAGGTTATCC TGGAATGGAT 
AGAGGACAAA CAGGAATGGA TAGAGGTCCT CCTGGAATGG ATAGAGGACA GCAAGGAATG 
GAGAGAGTTC CTCCTGGAAC AGACAAAAGA CTACCCGGAA TGGATAGAGG CCCTCCTGGA 
ATGGATGGAG GCCCTCCCAG ACTGGATAGA GGACAACCAG GCATGGATAA AGGCCCTCCT 
GGAATGAATA GAGGCCCTCC CGGAATGGAT GGAGGCCCTC CCGGAATGGA TAGAGGACAT 
CCAGGCATGG ATAAAGGCCC TCCTGGAATG GATAGAGGCC CTCCCGGAAT GGAGAGAGGC 
CCACCTGGAC TGGATAGAGG ACATCCAGGC ATGGATAAAG GCCCTCCTGG AATGGAGAGT 
GGACATCCAG GCATAGATAA AGGCCCTCCT GGAATGGATA GAGGTACAGC AGGAAGGAAT 
AGAGGCCAAT CAGAAATGGA TAGAGGCTCA TCAAGAAATG AGGGAGATGC ACCAAGAACT 
AATAAAGTTC AACTAGGGAT CGGGGGAATT CTTGCAGGAC TTAAAGATTC ATCAGGTATG 
GATAATGATC ATAGAATGAT TGACAGAGGC CCACCAGGAA TTGACAGTGG CCCTCCAGGA 
GCAGACAGTG GCCCACCAGG AGTAGACAGT GGTGCATTGA GAACCGATAG AAGTCAACTA 
GCTTTAGGGA ACGTTCTTGC TGGGTTGAGG GCAGCCCCAC CAGGAATGGA TAGAGGCCCA 
CCAGGAATAG ACGGTGGCCC ACCAGGAATA GACAGTGGCC CACCAGGAAT AGAAATAAAC 
GCCCCAGGTC GTACTAAAAG TCAACTAGGT TTAGGGAGCA TTCTTGCTGC ACTGAAGACA 
GGTCCACCAG GAATGGATAC GGGTCCACCT GGTGTGGAGA TGAGACCTAG TGGCATGGAC 
AAGGGACCCC CGGGAATAGA CATAGCCCCT CCAGTTATGG ATATGGGTCC TCCAGGAACG 
GACAGAGGAC CTCCATCTAA TTACATTAAA CCTCCAGGGA TGGACAACTC ACCTCCAGTT 
ATGGGGACAT TGCCCATGAG TTTGACCAGG GGTCCTCCTG GTCCACCTGG AGTTGATGTT 
GGACCTCCTG GTGTCGATGT TGGACCTCCT GGTGTGGATA TTGATGCAAT TAGTATGCAT 
AGCCATTATT TGGGAGCTGG AAAGCCTCGC CCTCCATTTG ATCAAAGGCA ATTGGATATT 
GGTCCACCAG GTGTAGATAC CTTTCCCCCT GGAATTCCAC GTTATCCTTT TGGTTCAACA 
CAAGGTCCAG CAGGATTTGG AAACTCATCT TCAATTCAAG ATAGCAGCTT TGAAGAGCCA 
ATGCCTCCTG GTATGGAGCC TATTAGTAAA CCCCTTGCTT TTCCCAGAGA TCTTGCACCT 
CCTGGGATGG ATGAGACATT TCCACCAGGG ATGGAACCAG AACAGGAAAG CCAGCCAAGC 
ACAAAAAGTA AACAGCCGCA ACCACCGGAA GACAATATAA GTGAATTTGA AAAGGGGCAA 
AACAAGCCAC TGTTTCCCCC ACCATCCAAA GGTTTGACTC AACATTCCTA TTCAGTTGGA 
CAATCCATAC CAATTACAGG TATGGTGAAA GATTCTTTTG GTGCCAGCTC CTTTGTTTCA 
ACAAAACAGA CATTAGCTCC AAGCCCACTT GCTGGGTATG AGTCATTAGA ATCGGTAAAG 
GCCAAGAAAG AAGAAAAGAA AAAAGATGAA TCAAAGTCAG AGGATAAAAA AAAAGGAAAA 
GGGAAAAAGG GAAATATGAA CATGCCTACA ATCATCAAAT TTGGGGATTC TAAAATTGAT 
AAAGATTCTG ATGATCCTGT CAAAGTTGAA AAGGACGAGA CATCTACTGA TGAAAAAGCA 
GCAAAGGAGG CTGCAAAAGC TAAGGAGTTT AAAAAAGAAA ATTCACCTTC TGAGGAGGTT 
TCAAAGAGAG TAACTGTGCG TAGCACCCTA CCATTGATCA CAGATTCTAC AACCAAGTCT 
ATGATACCTT CATCAATTGG TGAGCTATTC AAGAAGAACT CTACAACACC TCCAGCATTC 
AACAAAGATG TAAAGACCTC ACTTAGGAGC TACAGGAAAG CAGCAGAAGA TGATGAGGAA 
GAGGAAGAAG CGCCTCACTC TAAAAAGATG AATAGGAAGG ATTCTAGCGA TAGAGATGAT 
AGGGGACGGG ATGACACACT CCGCACTACA CTCCCATCCC CCCCACGGTC ATATCGGTCA 
CCTGATCGAA GGTCTAGGAG ATCTCCTGAA AGAAGGAGAT CTCGTTCTCC AGACAAAAGA 
AAGCGTAGAT CTCGGTCTAG ATCGATCTCA CAGGACCGCA GGAAACGGAG GCGGGATTCT 
CCACCCCGTA AGAGAAACTC ATACTCTCCG AGCAAAAGGA GGTCCCCCTC TAAAAGTAAA 
AATTCACGAT CCCCAAGCAG AGACAAGGGC AATAGATCCC CGGATCGAAG AGATCAGAGA 
GATCGAAGAG ATCAGAGAGA TCAAAGAGAT CAGAGAGATC GAAGAGATGG GAGAGATGAC 
AGATACAGAG AAAAGGACAG GAGGGACAGG TATCCTGACA AAGAATATGA GAAAGATTCT 
GATAGGAGAG GTGGGAAGCC AAGTACAGAA GCCTTTGACA AGTTGCGAGA GCTGATTTAT 
GATACCTTGG TGCGCCATCC TGATGAAAAG CCAAGGGATT CTCCACATAG CGATAGGCGA 
CACCCGGACG ATAGATCTCC TCAGCGACCA AGAAGGGAGA ACTATTACCC GGAAGCTGAG 
AGGCCAAGAA TGCGATACCC TCCACCACGT AGAGACTATG AAAGAGATCC TTTGAGGGAT 
CGGTTAAGAA GCCTTCCAAG GGATTCACCA AGGGATCTTC CATTGCCAGC TCAAAGGGAG 
CCTCGTAGGG ATTTTGATAG AGATTCCTCT CAAGACAGAT CTATGGAGAG GGCTCATTCC 
CCTCCGGTGA CGGAAATAAC AGCACCTATT GTCTTTGACT ATGGGCATGC AGAGTCCACC 
AGGCCTGCTT ATGACATAGA GACAAGAGAT CCCATGGGAA GCCTGCTCAG CAATGCAGTT 
ATTGTAGACG TTGTTGATTA TAGTCATGGT CAGAATCAGG GACCAGATGC TAGAGCAATT 
AGCGGATCAG AACTTCTTCA AGAGCGGGAG TCATCGTTCC CTTCAGAATT TCAAATACAT 
AAAGAGATTC AACCCGCAAA GAGAGATGAT ACCAGTATGC AACAATCACC AGCTCCACCA 
CATCGTGAAC CTTCACCTCA GCCTCCACCT CAGCCACCCA TGCAGCAATC ACTTCCCCAG 
CAAACTCAGC AGCAAGAACT TCCCTACAGA GAACAGCACC AGCAATTCTC TCAGCAGTAC 
CTGCCAGATG ATCTGACACA ACAGTTTGAT CCATACAGTG GCCAGCAACA GATTGGCTAT 
TACCAGCAAC CAGAATCACA GATGGCATTC CAACAACAAC AACACCTGCA AGCATTCCAG 
CAAATGGGCC AGTTCCCCCA GCAGGCTCAG TACAACACCC AGCAACAGCA GCAACACTAT 
ACGGAACAGT TCGAGCAGCA GCAGCAGCAG CCGCCGCCAC CACCACCTCC ACCATCTGAA 
GATTTGCGGT CAAGTGCCTA CCGCAAGTCC CCTCCCCCGC CGCCACCTGA TACTCCTGCC 
CCACCGAAGG ATGATTCCTT TCAGCAATCT TGGGCAGCTG CACCTCCCCC ACCTCCATCT 
GACTCTTACA CCAGTACCAG ACAATCTGGA GGTCAAGACC AAGACAGATC CAGGGAGAGA 
GGGGATGACA GGCAACGTGG AAGGGGTGAT AAGCAATCTG GAGGTCAAGA CCAAGACAGA 
TCCAGGGAGA GAGGAGATGA CAGGCAACGT GGAAGGGGGG ATAAATACCA GAGTGGCCCT 
GCCAGGCTAG TGGAGGACTA CAGGGACTCC CCTGGACAGA AGGACATGTT CACCAACTCA 
CCCCAGCAAA CCCCTCCTCC CCCTTCCAAG CCCAAGAAGA GGGAAGTGGT GGCAGTCACG 
GACCTCCTAG ACCCGCCCAA CAGGGAGAAA CGCCCAGCCA AGATCTGTAT GATCCTGCGT 
GGCCCTCCTG GAAGCGGCAA GAGTTACATT GCAAAGCTTA TCAAGGACAG AGAAATACAA 
AACCGAGGTC CTAGTCCTCG CATGCTGTGT CTGGATGACT ACTTTATGAG TGAGAAGGAG 
AAGACGGAAA AGGACGCTGA AACTGGCAAG ATTGTCAAGA AGAGGTTTCA GGAATACGAA 
CATGAGGACA GCATGGAGGC TATTTACCAT GTCAGTTTAC TCAAGACATT CAAGAAGACC 
ATCGAAGACG GTTTCTTTGA CTTCATCATC GTGGATGCCG TCAACGACAA AATCTCAAAG 
ATTGAACCGT TCTGGAGCTA TGCAAAGATG AAAGGATTTG AGGTGTATGT AGCTGAGTTA 
CTTGTTGAAT TAGGCTTGTG CATTCAGCGA GACATCCACA ATAGAGGTGA AACAGGAATC 
AGAAAGATCA TAGAACACTG GGAGTTTGCC CCACGGAAAT ACATCAAGCT AGATGTGGTG 
GCCCTCCTGC AAGATGCTGC CATTCCAGAG GTTGAGATGG AGGAACTTGA TGAAGACGCC 
GGACCTTCCA TGGAGGAACT GATGAACCAA CAGCAGGAAG CCTTGGAAGA ACTAGAGGAT 
CAGGAAGAGA CACTGGCTTT GACGAAGAGT AAGTGGGATT CTGCTACCGA AGAGAAACTA 
GATGCCCTTG ATGGTATCAA GAAGTATAAG AAGAAGGAGA GCTACACGTC TATGGAGGAC 
TTCCTATCAT GTGGAGATGA GTTTGACACA GTCTCCAAAC CCATACTCCC AGGACAGAAG 
AGAGTACGCT GGGCCGACAT CGAGGAACGC CAAAACCAAG AACGTATGCG TGCAGTAGGT 
TTTGTCGTTG GGCAGACGGC CACTGACTGG GCCAAGATCA CTGACGACAA TTATGCAGAC 
AAGGCTCTCA ATCGCACCAA GTACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC581149 ( XM_030999829.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030855689.1 Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X2, mRNA.

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CloneID	OSd65789
Clone ID Related Accession (Same CDS sequence)	XM_030999829.1
Accession Version	XM_030999829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	YLP motif-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GACCAGGTAT GAATATGATG GGACCAGGTG GACCAGGACC AAGAAGACCA 
CCTCCACCGA TGACGATGTC GATGATGCAT GGTCCTGGTC CAGGTGGCCC AAGGCCAAGA 
ATGGGTATGC CCATCCCAGA GCCTGTTCGG GTGTTAGTCA ATCAAGCGCA AGCCGCAGAT 
ACTGCAGTCC CACAACCGCC TCAGAATGAA GATGTGAAGT TGAGAGGGCT TCAGGATCAG 
GCCTTACATT GGCAGGCAAC GGCCCCGGTT CGAAAGGAGG AAGAAACTGT ACAAAAACAG 
GCAGCTGCTC AAGACCAGTG GCAGAGCTAC CAGCAACAGA TGCAATCATA TCAGCAACAG 
TATCAACAAT GGTACCAAAG TCAAGAGACT ACCTTGATGG ATGTTAAATT GAAAGACTTG 
CAGGAAAAGA AGGCTACCTG TGAAAAGCAG CTCACAGACT GGAAAAATCA GTTTGATAAT 
TGGGTAGAAC AGCATAAGAA ACATCCAAAC AAGGACCAGT TTGATCAATA TCAAAATCAA 
TGGAAAACAT GGCAAACACA GATGGAGAGC ATGGTGAATT CTTTGGACAT GCAAATCAAA 
ATAAAAGAGA CATCCCTTCA GAAGAACTCC TCTAAAAATG CTTCAACAAC CCAGACACCT 
GCTTCTGTCG GCAGTGGTGT AGGAACAGTT ACCACACAAG GTGCAAATGT AGCAATGCCA 
TCAGGTGGGC ATGGAATGGT TCCATCTAGT GGTGCTGGAA TGGGACAAGG GTCATCAATT 
GGTTCTATAC CAAGCAGTCA GAGTGTAGCC ACAGTTCCAA GTGGTTCTGG GATGGGAGCA 
GGACCCAGAG GACTTGGAGT AGGACCAATG CCAACTGGGA GTGGAATGGG ACCAGGTCCC 
TCAGGAACAA ATCCTAGAGT TACCCCTGCA TTGCCTCAAG GACCTGGGCA AGGGCCAAGA 
ATGGAGAGTG GAAGCAGGTC AAACTCTGGA GCTGGACTTC CAGGCCCAAA AGGACAGATG 
CCTGGCAAAG ACAGCTTTCC TTTCCCATTT CCTGATGATT TTCCATTTCC AGGAGATATG 
GACAATTTCT CCTTCCCTGA TATGAATAAA CTAGATGCAA TGTTTTCAGG TGGTTTTAAA 
TCTGATGAGT TTCCATTTTC CTTCCCTGAT TTTCCGATGC CAGGTGGTTT AATGGGACCC 
GACTTTCCAT TACCTGAAGG ACTGAAACCC GAAGACATGA CCAAAGATAT GGAAGAATTC 
ATGAAACAGA TGGAATCTGA ACTAGGCAAT GTTTCTGCAA AATTTCAACC AACCCCAAGG 
GGAAGCAACA TAGCCGATCA GGATAAACAG CAAGCTTTAA ATGCCAAAGC ATCAAGAGGA 
CCTGTAGACA TAGAGAGGGG TCCACAAGGA ATGAACAGAG ATTCTGCTGG GATGGGAAGA 
CACCCAATGG GAATGGATAG AGGTCCTCAA GGTATGGAGA GACACTCACA AGAATTCAAT 
AGAGGTCTAT CAGGAATAGA CAGAGGCCCA AGAGAAAGTC TTCAAGGGAG GGAAAGGGTC 
CCACCAGGAA TGGATAGAGG ACAACAAGGA ATGGATAGAT GCCCTCCCGG AATGGATAGA 
AGAAGACCAG GCATGGACGG AGGCCCTCTT GGAATGGATA GAGGACAACC AGGAATGGAT 
AGAGGACAAC CAGGAATGGA CAGAGGCCCT CTTGGAATGG ATAGAGGACA ACCAGAAATG 
GACAGAGGCC CTCTTGGAAT GGATAGAGGA CAACCAGGAA TTGACGGAGG CCCTCTTGGA 
ATGAATAGAG GACAACCAGG AATGGATAGA GGCACTCCTG GAATGGATAG AGGTCCCCCT 
GGGATGGATA GGGGACCAAC CGGAATGGAT AGAGGACAAC CAAGCTTGGA TAGAGGTCCT 
CCTGGAATGG ATAGAGGACA GCAAGGAATG GATAGAGGTC AACCAGGAAT GGATAGAGGA 
CAACCAGGAA TGGATAGAGG ACAACCAGGA ATGGATAGAG GTCCTCCTGG AATGGATAGA 
GGACAGCAAC GAATGGATAG AGGACAGCAA GGAATGGATA GAGGTTATCC TGGAATGGAT 
AGAGGACAAA CAGGAATGGA TAGAGGTCCT CCTGGAATGG ATAGAGGACA GCAAGGAATG 
GAGAGAGTTC CTCCTGGAAC AGACAAAAGA CTACCCGGAA TGGATAGAGG CCCTCCTGGA 
ATGGATGGAG GCCCTCCCAG ACTGGATAGA GGACAACCAG GCATGGATAA AGGCCCTCCT 
GGAATGAATA GAGGCCCTCC CGGAATGGAT GGAGGCCCTC CCGGAATGGA TAGAGGACAT 
CCAGGCATGG ATAAAGGCCC TCCTGGAATG GATAGAGGCC CTCCCGGAAT GGAGAGAGGC 
CCACCTGGAC TGGATAGAGG ACATCCAGGC ATGGATAAAG GCCCTCCTGG AATGGAGAGT 
GGACATCCAG GCATAGATAA AGGCCCTCCT GGAATGGATA GAGGTACAGC AGGAAGGAAT 
AGAGGCCAAT CAGAAATGGA TAGAGGCTCA TCAAGAAATG AGGGAGATGC ACCAAGAACT 
AATAAAGTTC AACTAGGGAT CGGGGGAATT CTTGCAGGAC TTAAAGATTC ATCAGGTATG 
GATAATGATC ATAGAATGAT TGACAGAGGC CCACCAGGAA TTGACAGTGG CCCTCCAGGA 
GCAGACAGTG GCCCACCAGG AGTAGACAGT GGTGCATTGA GAACCGATAG AAGTCAACTA 
GCTTTAGGGA ACGTTCTTGC TGGGTTGAGG GCAGCCCCAC CAGGAATGGA TAGAGGCCCA 
CCAGGAATAG ACGGTGGCCC ACCAGGAATA GACAGTGGCC CACCAGGAAT AGAAATAAAC 
GCCCCAGGTC GTACTAAAAG TCAACTAGGT TTAGGGAGCA TTCTTGCTGC ACTGAAGACA 
GGTCCACCAG GAATGGATAC GGGTCCACCT GGTGTGGAGA TGAGACCTAG TGGCATGGAC 
AAGGGACCCC CGGGAATAGA CATAGCCCCT CCAGTTATGG ATATGGGTCC TCCAGGAACG 
GACAGAGGAC CTCCATCTAA TTACATTAAA CCTCCAGGGA TGGACAACTC ACCTCCAGTT 
ATGGGGACAT TGCCCATGAG TTTGACCAGG GGTCCTCCTG GTCCACCTGG AGTTGATGTT 
GGACCTCCTG GTGTCGATGT TGGACCTCCT GGTGTGGATA TTGATGCAAT TAGTATGCAT 
AGCCATTATT TGGGAGCTGG AAAGCCTCGC CCTCCATTTG ATCAAAGGCA ATTGGATATT 
GGTCCACCAG GTGTAGATAC CTTTCCCCCT GGAATTCCAC GTTATCCTTT TGGTTCAACA 
CAAGGTCCAG CAGGATTTGG AAACTCATCT TCAATTCAAG ATAGCAGCTT TGAAGAGCCA 
ATGCCTCCTG GTATGGAGCC TATTAGTAAA CCCCTTGCTT TTCCCAGAGA TCTTGCACCT 
CCTGGGATGG ATGAGACATT TCCACCAGGG ATGGAACCAG AACAGGAAAG CCAGCCAAGC 
ACAAAAAGTA AACAGCCGCA ACCACCGGAA GACAATATAA GTGAATTTGA AAAGGGGCAA 
AACAAGCCAC TGTTTCCCCC ACCATCCAAA GGTTTGACTC AACATTCCTA TTCAGTTGGA 
CAATCCATAC CAATTACAGG TATGGTGAAA GATTCTTTTG GTGCCAGCTC CTTTGTTTCA 
ACAAAACAGA CATTAGCTCC AAGCCCACTT GCTGGGTATG AGTCATTAGA ATCGGTAAAG 
GCCAAGAAAG AAGAAAAGAA AAAAGATGAA TCAAAGTCAG AGGATAAAAA AAAAGGAAAA 
GGGAAAAAGG GAAATATGAA CATGCCTACA ATCATCAAAT TTGGGGATTC TAAAATTGAT 
AAAGATTCTG ATGATCCTGT CAAAGTTGAA AAGGACGAGA CATCTACTGA TGAAAAAGCA 
GCAAAGGAGG CTGCAAAAGC TAAGGAGTTT AAAAAAGAAA ATTCACCTTC TGAGGAGGTT 
TCAAAGAGAG TAACTGTGCG TAGCACCCTA CCATTGATCA CAGATTCTAC AACCAAGTCT 
ATGATACCTT CATCAATTGG TGAGCTATTC AAGAAGAACT CTACAACACC TCCAGCATTC 
AACAAAGATG TAAAGACCTC ACTTAGGAGC TACAGGAAAG CAGCAGAAGA TGATGAGGAA 
GAGGAAGAAG CGCCTCACTC TAAAAAGATG AATAGGAAGG ATTCTAGCGA TAGAGATGAT 
AGGGGACGGG ATGACACACT CCGCACTACA CTCCCATCCC CCCCACGGTC ATATCGGTCA 
CCTGATCGAA GGTCTAGGAG ATCTCCTGAA AGAAGGAGAT CTCGTTCTCC AGACAAAAGA 
AAGCGTAGAT CTCGGTCTAG ATCGATCTCA CAGGACCGCA GGAAACGGAG GCGGGATTCT 
CCACCCCGTA AGAGAAACTC ATACTCTCCG AGCAAAAGGA GGTCCCCCTC TAAAAGTAAA 
AATTCACGAT CCCCAAGCAG AGACAAGGGC AATAGATCCC CGGATCGAAG AGATCAGAGA 
GATCGAAGAG ATCAGAGAGA TCAAAGAGAT CAGAGAGATC GAAGAGATGG GAGAGATGAC 
AGATACAGAG AAAAGGACAG GAGGGACAGG TATCCTGACA AAGAATATGA GAAAGATTCT 
GATAGGAGAG GTGGGAAGCC AAGTACAGAA GCCTTTGACA AGTTGCGAGA GCTGATTTAT 
GATACCTTGG TGCGCCATCC TGATGAAAAG CCAAGGGATT CTCCACATAG CGATAGGCGA 
CACCCGGACG ATAGATCTCC TCAGCGACCA AGAAGGGAGA ACTATTACCC GGAAGCTGAG 
AGGCCAAGAA TGCGATACCC TCCACCACGT AGAGACTATG AAAGAGATCC TTTGAGGGAT 
CGGTTAAGAA GCCTTCCAAG GGATTCACCA AGGGATCTTC CATTGCCAGC TCAAAGGGAG 
CCTCGTAGGG ATTTTGATAG AGATTCCTCT CAAGACAGAT CTATGGAGAG GGCTCATTCC 
CCTCCGGTGA CGGAAATAAC AGCACCTATT GTCTTTGACT ATGGGCATGC AGAGTCCACC 
AGGCCTGCTT ATGACATAGA GACAAGAGAT CCCATGGGAA GCCTGCTCAG CAATGCAGTT 
ATTGTAGACG TTGTTGATTA TAGTCATGGT CAGAATCAGG GACCAGATGC TAGAGCAATT 
AGCGGATCAG AACTTCTTCA AGAGCGGGAG TCATCGTTCC CTTCAGAATT TCAAATACAT 
AAAGAGATTC AACCCGCAAA GAGAGATGAT ACCAGTATGC AACAATCACC AGCTCCACCA 
CATCGTGAAC CTTCACCTCA GCCTCCACCT CAGCCACCCA TGCAGCAATC ACTTCCCCAG 
CAAACTCAGC AGCAAGAACT TCCCTACAGA GAACAGCACC AGCAATTCTC TCAGCAGTAC 
CTGCCAGATG ATCTGACACA ACAGTTTGAT CCATACAGTG GCCAGCAACA GATTGGCTAT 
TACCAGCAAC CAGAATCACA GATGGCATTC CAACAACAAC AACACCTGCA AGCATTCCAG 
CAAATGGGCC AGTTCCCCCA GCAGGCTCAG TACAACACCC AGCAACAGCA GCAACACTAT 
ACGGAACAGT TCGAGCAGCA GCAGCAGCAG CCGCCGCCAC CACCACCTCC ACCATCTGAA 
GATTTGCGGT CAAGTGCCTA CCGCAAGTCC CCTCCCCCGC CGCCACCTGA TACTCCTGCC 
CCACCGAAGG ATGATTCCTT TCAGCAATCT TGGGCAGCTG CACCTCCCCC ACCTCCATCT 
GACTCTTACA CCAGTACCAG ACAATCTGGA GGTCAAGACC AAGACAGATC CAGGGAGAGA 
GGGGATGACA GGCAACGTGG AAGGGGTGAT AAATACCAGA GTGGCCCTGC CAGGCTAGTG 
GAGGACTACA GGGACTCCCC TGGACAGAAG GACATGTTCA CCAACTCACC CCAGCAAACC 
CCTCCTCCCC CTTCCAAGCC CAAGAAGAGG GAAGTGGTGG CAGTCACGGA CCTCCTAGAC 
CCGCCCAACA GGGAGAAACG CCCAGCCAAG ATCTGTATGA TCCTGCGTGG CCCTCCTGGA 
AGCGGCAAGA GTTACATTGC AAAGCTTATC AAGGACAGAG AAATACAAAA CCGAGGTCCT 
AGTCCTCGCA TGCTGTGTCT GGATGACTAC TTTATGAGTG AGAAGGAGAA GACGGAAAAG 
GACGCTGAAA CTGGCAAGAT TGTCAAGAAG AGGTTTCAGG AATACGAACA TGAGGACAGC 
ATGGAGGCTA TTTACCATGT CAGTTTACTC AAGACATTCA AGAAGACCAT CGAAGACGGT 
TTCTTTGACT TCATCATCGT GGATGCCGTC AACGACAAAA TCTCAAAGAT TGAACCGTTC 
TGGAGCTATG CAAAGATGAA AGGATTTGAG GTGTATGTAG CTGAGTTACT TGTTGAATTA 
GGCTTGTGCA TTCAGCGAGA CATCCACAAT AGAGGTGAAA CAGGAATCAG AAAGATCATA 
GAACACTGGG AGTTTGCCCC ACGGAAATAC ATCAAGCTAG ATGTGGTGGC CCTCCTGCAA 
GATGCTGCCA TTCCAGAGGT TGAGATGGAG GAACTTGATG AAGACGCCGG ACCTTCCATG 
GAGGAACTGA TGAACCAACA GCAGGAAGCC TTGGAAGAAC TAGAGGATCA GGAAGAGACA 
CTGGCTTTGA CGAAGAGTAA GTGGGATTCT GCTACCGAAG AGAAACTAGA TGCCCTTGAT 
GGTATCAAGA AGTATAAGAA GAAGGAGAGC TACACGTCTA TGGAGGACTT CCTATCATGT 
GGAGATGAGT TTGACACAGT CTCCAAACCC ATACTCCCAG GACAGAAGAG AGTACGCTGG 
GCCGACATCG AGGAACGCCA AAACCAAGAA CGTATGCGTG CAGTAGGTTT TGTCGTTGGG 
CAGACGGCCA CTGACTGGGC CAAGATCACT GACGACAATT ATGCAGACAA GGCTCTCAAT 
CGCACCAAGT ACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030855689.1
CDS	124..7401
Translation	MFNNWNRPGGPGPGGPGERPPMMPGNGMPNRPPFYGPGSGPGAGGRPMHMSGPPGPGPGSHHMGMRMDSMNPRGPMSGPGHWHGPGMNMMGPGGPGPRRPPPPMTMSMMHGPGPGGPRPRMGMPIPEPVRVLVNQAQAADTAVPQPPQNEDVKLRGLQDQALHWQATAPVRKEEETVQKQAAAQDQWQSYQQQMQSYQQQYQQWYQSQETTLMDVKLKDLQEKKATCEKQLTDWKNQFDNWVEQHKKHPNKDQFDQYQNQWKTWQTQMESMVNSLDMQIKIKETSLQKNSSKNASTTQTPASVGSGVGTVTTQGANVAMPSGGHGMVPSSGAGMGQGSSIGSIPSSQSVATVPSGSGMGAGPRGLGVGPMPTGSGMGPGPSGTNPRVTPALPQGPGQGPRMESGSRSNSGAGLPGPKGQMPGKDSFPFPFPDDFPFPGDMDNFSFPDMNKLDAMFSGGFKSDEFPFSFPDFPMPGGLMGPDFPLPEGLKPEDMTKDMEEFMKQMESELGNVSAKFQPTPRGSNIADQDKQQALNAKASRGPVDIERGPQGMNRDSAGMGRHPMGMDRGPQGMERHSQEFNRGLSGIDRGPRESLQGRERVPPGMDRGQQGMDRCPPGMDRRRPGMDGGPLGMDRGQPGMDRGQPGMDRGPLGMDRGQPEMDRGPLGMDRGQPGIDGGPLGMNRGQPGMDRGTPGMDRGPPGMDRGPTGMDRGQPSLDRGPPGMDRGQQGMDRGQPGMDRGQPGMDRGQPGMDRGPPGMDRGQQRMDRGQQGMDRGYPGMDRGQTGMDRGPPGMDRGQQGMERVPPGTDKRLPGMDRGPPGMDGGPPRLDRGQPGMDKGPPGMNRGPPGMDGGPPGMDRGHPGMDKGPPGMDRGPPGMERGPPGLDRGHPGMDKGPPGMESGHPGIDKGPPGMDRGTAGRNRGQSEMDRGSSRNEGDAPRTNKVQLGIGGILAGLKDSSGMDNDHRMIDRGPPGIDSGPPGADSGPPGVDSGALRTDRSQLALGNVLAGLRAAPPGMDRGPPGIDGGPPGIDSGPPGIEINAPGRTKSQLGLGSILAALKTGPPGMDTGPPGVEMRPSGMDKGPPGIDIAPPVMDMGPPGTDRGPPSNYIKPPGMDNSPPVMGTLPMSLTRGPPGPPGVDVGPPGVDVGPPGVDIDAISMHSHYLGAGKPRPPFDQRQLDIGPPGVDTFPPGIPRYPFGSTQGPAGFGNSSSIQDSSFEEPMPPGMEPISKPLAFPRDLAPPGMDETFPPGMEPEQESQPSTKSKQPQPPEDNISEFEKGQNKPLFPPPSKGLTQHSYSVGQSIPITGMVKDSFGASSFVSTKQTLAPSPLAGYESLESVKAKKEEKKKDESKSEDKKKGKGKKGNMNMPTIIKFGDSKIDKDSDDPVKVEKDETSTDEKAAKEAAKAKEFKKENSPSEEVSKRVTVRSTLPLITDSTTKSMIPSSIGELFKKNSTTPPAFNKDVKTSLRSYRKAAEDDEEEEEAPHSKKMNRKDSSDRDDRGRDDTLRTTLPSPPRSYRSPDRRSRRSPERRRSRSPDKRKRRSRSRSISQDRRKRRRDSPPRKRNSYSPSKRRSPSKSKNSRSPSRDKGNRSPDRRDQRDRRDQRDQRDQRDRRDGRDDRYREKDRRDRYPDKEYEKDSDRRGGKPSTEAFDKLRELIYDTLVRHPDEKPRDSPHSDRRHPDDRSPQRPRRENYYPEAERPRMRYPPPRRDYERDPLRDRLRSLPRDSPRDLPLPAQREPRRDFDRDSSQDRSMERAHSPPVTEITAPIVFDYGHAESTRPAYDIETRDPMGSLLSNAVIVDVVDYSHGQNQGPDARAISGSELLQERESSFPSEFQIHKEIQPAKRDDTSMQQSPAPPHREPSPQPPPQPPMQQSLPQQTQQQELPYREQHQQFSQQYLPDDLTQQFDPYSGQQQIGYYQQPESQMAFQQQQHLQAFQQMGQFPQQAQYNTQQQQQHYTEQFEQQQQQPPPPPPPPSEDLRSSAYRKSPPPPPPDTPAPPKDDSFQQSWAAAPPPPPSDSYTSTRQSGGQDQDRSRERGDDRQRGRGDKYQSGPARLVEDYRDSPGQKDMFTNSPQQTPPPPSKPKKREVVAVTDLLDPPNREKRPAKICMILRGPPGSGKSYIAKLIKDREIQNRGPSPRMLCLDDYFMSEKEKTEKDAETGKIVKKRFQEYEHEDSMEAIYHVSLLKTFKKTIEDGFFDFIIVDAVNDKISKIEPFWSYAKMKGFEVYVAELLVELGLCIQRDIHNRGETGIRKIIEHWEFAPRKYIKLDVVALLQDAAIPEVEMEELDEDAGPSMEELMNQQQEALEELEDQEETLALTKSKWDSATEEKLDALDGIKKYKKKESYTSMEDFLSCGDEFDTVSKPILPGQKRVRWADIEERQNQERMRAVGFVVGQTATDWAKITDDNYADKALNRTKYI

Target ORF information:

RefSeq Version	XM_030999829.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030999829.1

ORF Insert Sequence:

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GACCAGGTAT GAATATGATG GGACCAGGTG GACCAGGACC AAGAAGACCA 
CCTCCACCGA TGACGATGTC GATGATGCAT GGTCCTGGTC CAGGTGGCCC AAGGCCAAGA 
ATGGGTATGC CCATCCCAGA GCCTGTTCGG GTGTTAGTCA ATCAAGCGCA AGCCGCAGAT 
ACTGCAGTCC CACAACCGCC TCAGAATGAA GATGTGAAGT TGAGAGGGCT TCAGGATCAG 
GCCTTACATT GGCAGGCAAC GGCCCCGGTT CGAAAGGAGG AAGAAACTGT ACAAAAACAG 
GCAGCTGCTC AAGACCAGTG GCAGAGCTAC CAGCAACAGA TGCAATCATA TCAGCAACAG 
TATCAACAAT GGTACCAAAG TCAAGAGACT ACCTTGATGG ATGTTAAATT GAAAGACTTG 
CAGGAAAAGA AGGCTACCTG TGAAAAGCAG CTCACAGACT GGAAAAATCA GTTTGATAAT 
TGGGTAGAAC AGCATAAGAA ACATCCAAAC AAGGACCAGT TTGATCAATA TCAAAATCAA 
TGGAAAACAT GGCAAACACA GATGGAGAGC ATGGTGAATT CTTTGGACAT GCAAATCAAA 
ATAAAAGAGA CATCCCTTCA GAAGAACTCC TCTAAAAATG CTTCAACAAC CCAGACACCT 
GCTTCTGTCG GCAGTGGTGT AGGAACAGTT ACCACACAAG GTGCAAATGT AGCAATGCCA 
TCAGGTGGGC ATGGAATGGT TCCATCTAGT GGTGCTGGAA TGGGACAAGG GTCATCAATT 
GGTTCTATAC CAAGCAGTCA GAGTGTAGCC ACAGTTCCAA GTGGTTCTGG GATGGGAGCA 
GGACCCAGAG GACTTGGAGT AGGACCAATG CCAACTGGGA GTGGAATGGG ACCAGGTCCC 
TCAGGAACAA ATCCTAGAGT TACCCCTGCA TTGCCTCAAG GACCTGGGCA AGGGCCAAGA 
ATGGAGAGTG GAAGCAGGTC AAACTCTGGA GCTGGACTTC CAGGCCCAAA AGGACAGATG 
CCTGGCAAAG ACAGCTTTCC TTTCCCATTT CCTGATGATT TTCCATTTCC AGGAGATATG 
GACAATTTCT CCTTCCCTGA TATGAATAAA CTAGATGCAA TGTTTTCAGG TGGTTTTAAA 
TCTGATGAGT TTCCATTTTC CTTCCCTGAT TTTCCGATGC CAGGTGGTTT AATGGGACCC 
GACTTTCCAT TACCTGAAGG ACTGAAACCC GAAGACATGA CCAAAGATAT GGAAGAATTC 
ATGAAACAGA TGGAATCTGA ACTAGGCAAT GTTTCTGCAA AATTTCAACC AACCCCAAGG 
GGAAGCAACA TAGCCGATCA GGATAAACAG CAAGCTTTAA ATGCCAAAGC ATCAAGAGGA 
CCTGTAGACA TAGAGAGGGG TCCACAAGGA ATGAACAGAG ATTCTGCTGG GATGGGAAGA 
CACCCAATGG GAATGGATAG AGGTCCTCAA GGTATGGAGA GACACTCACA AGAATTCAAT 
AGAGGTCTAT CAGGAATAGA CAGAGGCCCA AGAGAAAGTC TTCAAGGGAG GGAAAGGGTC 
CCACCAGGAA TGGATAGAGG ACAACAAGGA ATGGATAGAT GCCCTCCCGG AATGGATAGA 
AGAAGACCAG GCATGGACGG AGGCCCTCTT GGAATGGATA GAGGACAACC AGGAATGGAT 
AGAGGACAAC CAGGAATGGA CAGAGGCCCT CTTGGAATGG ATAGAGGACA ACCAGAAATG 
GACAGAGGCC CTCTTGGAAT GGATAGAGGA CAACCAGGAA TTGACGGAGG CCCTCTTGGA 
ATGAATAGAG GACAACCAGG AATGGATAGA GGCACTCCTG GAATGGATAG AGGTCCCCCT 
GGGATGGATA GGGGACCAAC CGGAATGGAT AGAGGACAAC CAAGCTTGGA TAGAGGTCCT 
CCTGGAATGG ATAGAGGACA GCAAGGAATG GATAGAGGTC AACCAGGAAT GGATAGAGGA 
CAACCAGGAA TGGATAGAGG ACAACCAGGA ATGGATAGAG GTCCTCCTGG AATGGATAGA 
GGACAGCAAC GAATGGATAG AGGACAGCAA GGAATGGATA GAGGTTATCC TGGAATGGAT 
AGAGGACAAA CAGGAATGGA TAGAGGTCCT CCTGGAATGG ATAGAGGACA GCAAGGAATG 
GAGAGAGTTC CTCCTGGAAC AGACAAAAGA CTACCCGGAA TGGATAGAGG CCCTCCTGGA 
ATGGATGGAG GCCCTCCCAG ACTGGATAGA GGACAACCAG GCATGGATAA AGGCCCTCCT 
GGAATGAATA GAGGCCCTCC CGGAATGGAT GGAGGCCCTC CCGGAATGGA TAGAGGACAT 
CCAGGCATGG ATAAAGGCCC TCCTGGAATG GATAGAGGCC CTCCCGGAAT GGAGAGAGGC 
CCACCTGGAC TGGATAGAGG ACATCCAGGC ATGGATAAAG GCCCTCCTGG AATGGAGAGT 
GGACATCCAG GCATAGATAA AGGCCCTCCT GGAATGGATA GAGGTACAGC AGGAAGGAAT 
AGAGGCCAAT CAGAAATGGA TAGAGGCTCA TCAAGAAATG AGGGAGATGC ACCAAGAACT 
AATAAAGTTC AACTAGGGAT CGGGGGAATT CTTGCAGGAC TTAAAGATTC ATCAGGTATG 
GATAATGATC ATAGAATGAT TGACAGAGGC CCACCAGGAA TTGACAGTGG CCCTCCAGGA 
GCAGACAGTG GCCCACCAGG AGTAGACAGT GGTGCATTGA GAACCGATAG AAGTCAACTA 
GCTTTAGGGA ACGTTCTTGC TGGGTTGAGG GCAGCCCCAC CAGGAATGGA TAGAGGCCCA 
CCAGGAATAG ACGGTGGCCC ACCAGGAATA GACAGTGGCC CACCAGGAAT AGAAATAAAC 
GCCCCAGGTC GTACTAAAAG TCAACTAGGT TTAGGGAGCA TTCTTGCTGC ACTGAAGACA 
GGTCCACCAG GAATGGATAC GGGTCCACCT GGTGTGGAGA TGAGACCTAG TGGCATGGAC 
AAGGGACCCC CGGGAATAGA CATAGCCCCT CCAGTTATGG ATATGGGTCC TCCAGGAACG 
GACAGAGGAC CTCCATCTAA TTACATTAAA CCTCCAGGGA TGGACAACTC ACCTCCAGTT 
ATGGGGACAT TGCCCATGAG TTTGACCAGG GGTCCTCCTG GTCCACCTGG AGTTGATGTT 
GGACCTCCTG GTGTCGATGT TGGACCTCCT GGTGTGGATA TTGATGCAAT TAGTATGCAT 
AGCCATTATT TGGGAGCTGG AAAGCCTCGC CCTCCATTTG ATCAAAGGCA ATTGGATATT 
GGTCCACCAG GTGTAGATAC CTTTCCCCCT GGAATTCCAC GTTATCCTTT TGGTTCAACA 
CAAGGTCCAG CAGGATTTGG AAACTCATCT TCAATTCAAG ATAGCAGCTT TGAAGAGCCA 
ATGCCTCCTG GTATGGAGCC TATTAGTAAA CCCCTTGCTT TTCCCAGAGA TCTTGCACCT 
CCTGGGATGG ATGAGACATT TCCACCAGGG ATGGAACCAG AACAGGAAAG CCAGCCAAGC 
ACAAAAAGTA AACAGCCGCA ACCACCGGAA GACAATATAA GTGAATTTGA AAAGGGGCAA 
AACAAGCCAC TGTTTCCCCC ACCATCCAAA GGTTTGACTC AACATTCCTA TTCAGTTGGA 
CAATCCATAC CAATTACAGG TATGGTGAAA GATTCTTTTG GTGCCAGCTC CTTTGTTTCA 
ACAAAACAGA CATTAGCTCC AAGCCCACTT GCTGGGTATG AGTCATTAGA ATCGGTAAAG 
GCCAAGAAAG AAGAAAAGAA AAAAGATGAA TCAAAGTCAG AGGATAAAAA AAAAGGAAAA 
GGGAAAAAGG GAAATATGAA CATGCCTACA ATCATCAAAT TTGGGGATTC TAAAATTGAT 
AAAGATTCTG ATGATCCTGT CAAAGTTGAA AAGGACGAGA CATCTACTGA TGAAAAAGCA 
GCAAAGGAGG CTGCAAAAGC TAAGGAGTTT AAAAAAGAAA ATTCACCTTC TGAGGAGGTT 
TCAAAGAGAG TAACTGTGCG TAGCACCCTA CCATTGATCA CAGATTCTAC AACCAAGTCT 
ATGATACCTT CATCAATTGG TGAGCTATTC AAGAAGAACT CTACAACACC TCCAGCATTC 
AACAAAGATG TAAAGACCTC ACTTAGGAGC TACAGGAAAG CAGCAGAAGA TGATGAGGAA 
GAGGAAGAAG CGCCTCACTC TAAAAAGATG AATAGGAAGG ATTCTAGCGA TAGAGATGAT 
AGGGGACGGG ATGACACACT CCGCACTACA CTCCCATCCC CCCCACGGTC ATATCGGTCA 
CCTGATCGAA GGTCTAGGAG ATCTCCTGAA AGAAGGAGAT CTCGTTCTCC AGACAAAAGA 
AAGCGTAGAT CTCGGTCTAG ATCGATCTCA CAGGACCGCA GGAAACGGAG GCGGGATTCT 
CCACCCCGTA AGAGAAACTC ATACTCTCCG AGCAAAAGGA GGTCCCCCTC TAAAAGTAAA 
AATTCACGAT CCCCAAGCAG AGACAAGGGC AATAGATCCC CGGATCGAAG AGATCAGAGA 
GATCGAAGAG ATCAGAGAGA TCAAAGAGAT CAGAGAGATC GAAGAGATGG GAGAGATGAC 
AGATACAGAG AAAAGGACAG GAGGGACAGG TATCCTGACA AAGAATATGA GAAAGATTCT 
GATAGGAGAG GTGGGAAGCC AAGTACAGAA GCCTTTGACA AGTTGCGAGA GCTGATTTAT 
GATACCTTGG TGCGCCATCC TGATGAAAAG CCAAGGGATT CTCCACATAG CGATAGGCGA 
CACCCGGACG ATAGATCTCC TCAGCGACCA AGAAGGGAGA ACTATTACCC GGAAGCTGAG 
AGGCCAAGAA TGCGATACCC TCCACCACGT AGAGACTATG AAAGAGATCC TTTGAGGGAT 
CGGTTAAGAA GCCTTCCAAG GGATTCACCA AGGGATCTTC CATTGCCAGC TCAAAGGGAG 
CCTCGTAGGG ATTTTGATAG AGATTCCTCT CAAGACAGAT CTATGGAGAG GGCTCATTCC 
CCTCCGGTGA CGGAAATAAC AGCACCTATT GTCTTTGACT ATGGGCATGC AGAGTCCACC 
AGGCCTGCTT ATGACATAGA GACAAGAGAT CCCATGGGAA GCCTGCTCAG CAATGCAGTT 
ATTGTAGACG TTGTTGATTA TAGTCATGGT CAGAATCAGG GACCAGATGC TAGAGCAATT 
AGCGGATCAG AACTTCTTCA AGAGCGGGAG TCATCGTTCC CTTCAGAATT TCAAATACAT 
AAAGAGATTC AACCCGCAAA GAGAGATGAT ACCAGTATGC AACAATCACC AGCTCCACCA 
CATCGTGAAC CTTCACCTCA GCCTCCACCT CAGCCACCCA TGCAGCAATC ACTTCCCCAG 
CAAACTCAGC AGCAAGAACT TCCCTACAGA GAACAGCACC AGCAATTCTC TCAGCAGTAC 
CTGCCAGATG ATCTGACACA ACAGTTTGAT CCATACAGTG GCCAGCAACA GATTGGCTAT 
TACCAGCAAC CAGAATCACA GATGGCATTC CAACAACAAC AACACCTGCA AGCATTCCAG 
CAAATGGGCC AGTTCCCCCA GCAGGCTCAG TACAACACCC AGCAACAGCA GCAACACTAT 
ACGGAACAGT TCGAGCAGCA GCAGCAGCAG CCGCCGCCAC CACCACCTCC ACCATCTGAA 
GATTTGCGGT CAAGTGCCTA CCGCAAGTCC CCTCCCCCGC CGCCACCTGA TACTCCTGCC 
CCACCGAAGG ATGATTCCTT TCAGCAATCT TGGGCAGCTG CACCTCCCCC ACCTCCATCT 
GACTCTTACA CCAGTACCAG ACAATCTGGA GGTCAAGACC AAGACAGATC CAGGGAGAGA 
GGGGATGACA GGCAACGTGG AAGGGGTGAT AAATACCAGA GTGGCCCTGC CAGGCTAGTG 
GAGGACTACA GGGACTCCCC TGGACAGAAG GACATGTTCA CCAACTCACC CCAGCAAACC 
CCTCCTCCCC CTTCCAAGCC CAAGAAGAGG GAAGTGGTGG CAGTCACGGA CCTCCTAGAC 
CCGCCCAACA GGGAGAAACG CCCAGCCAAG ATCTGTATGA TCCTGCGTGG CCCTCCTGGA 
AGCGGCAAGA GTTACATTGC AAAGCTTATC AAGGACAGAG AAATACAAAA CCGAGGTCCT 
AGTCCTCGCA TGCTGTGTCT GGATGACTAC TTTATGAGTG AGAAGGAGAA GACGGAAAAG 
GACGCTGAAA CTGGCAAGAT TGTCAAGAAG AGGTTTCAGG AATACGAACA TGAGGACAGC 
ATGGAGGCTA TTTACCATGT CAGTTTACTC AAGACATTCA AGAAGACCAT CGAAGACGGT 
TTCTTTGACT TCATCATCGT GGATGCCGTC AACGACAAAA TCTCAAAGAT TGAACCGTTC 
TGGAGCTATG CAAAGATGAA AGGATTTGAG GTGTATGTAG CTGAGTTACT TGTTGAATTA 
GGCTTGTGCA TTCAGCGAGA CATCCACAAT AGAGGTGAAA CAGGAATCAG AAAGATCATA 
GAACACTGGG AGTTTGCCCC ACGGAAATAC ATCAAGCTAG ATGTGGTGGC CCTCCTGCAA 
GATGCTGCCA TTCCAGAGGT TGAGATGGAG GAACTTGATG AAGACGCCGG ACCTTCCATG 
GAGGAACTGA TGAACCAACA GCAGGAAGCC TTGGAAGAAC TAGAGGATCA GGAAGAGACA 
CTGGCTTTGA CGAAGAGTAA GTGGGATTCT GCTACCGAAG AGAAACTAGA TGCCCTTGAT 
GGTATCAAGA AGTATAAGAA GAAGGAGAGC TACACGTCTA TGGAGGACTT CCTATCATGT 
GGAGATGAGT TTGACACAGT CTCCAAACCC ATACTCCCAG GACAGAAGAG AGTACGCTGG 
GCCGACATCG AGGAACGCCA AAACCAAGAA CGTATGCGTG CAGTAGGTTT TGTCGTTGGG 
CAGACGGCCA CTGACTGGGC CAAGATCACT GACGACAATT ATGCAGACAA GGCTCTCAAT 
CGCACCAAGT ACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC581149 ( XM_030999830.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030855690.1 Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X3, mRNA.

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CloneID	OSd65790
Clone ID Related Accession (Same CDS sequence)	XM_030999830.1
Accession Version	XM_030999830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	YLP motif-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GACCAGGTAT GAATATGATG GGACCAGGTG GACCAGGACC AAGAAGACCA 
CCTCCACCGA TGACGATGTC GATGATGCAT GGTCCTGGTC CAGGTGGCCC AAGGCCAAGA 
ATGGGTATGC CCATCCCAGA GCCTGTTCGG GTGTTAGTCA ATCAAGCGCA AGCCGCAGAT 
ACTGCAGTCC CACAACCGCC TCAGAATGAA GATGTGAAGT TGAGAGGGCT TCAGGATCAG 
GCCTTACATT GGCAGGCAAC GGCCCCGGTT CGAAAGGAGG AAGAAACTGT ACAAAAACAG 
GCAGCTGCTC AAGACCAGTG GCAGAGCTAC CAGCAACAGA TGCAATCATA TCAGCAACAG 
TATCAACAAT GGTACCAAAG TCAAGAGACT ACCTTGATGG ATGTTAAATT GAAAGACTTG 
CAGGAAAAGA AGGCTACCTG TGAAAAGCAG CTCACAGACT GGAAAAATCA GTTTGATAAT 
TGGGTAGAAC AGCATAAGAA ACATCCAAAC AAGGACCAGT TTGATCAATA TCAAAATCAA 
TGGAAAACAT GGCAAACACA GATGGAGAGC ATGGTGAATT CTTTGGACAT GCAAATCAAA 
ATAAAAGAGA CATCCCTTCA GAAGAACTCC TCTAAAAATG CTTCAACAAC CCAGACACCT 
GCTTCTGTCG GCAGTGGTGT AGGAACAGTT ACCACACAAG GTGCAAATGT AGCAATGCCA 
TCAGGTGGGC ATGGAATGGT TCCATCTAGT GGTGCTGGAA TGGGACAAGG GTCATCAATT 
GGTTCTATAC CAAGCAGTCA GAGTGTAGCC ACAGTTCCAA GTGGTTCTGG GATGGGAGCA 
GGACCCAGAG GACTTGGAGT AGGACCAATG CCAACTGGGA GTGGAATGGG ACCAGGTCCC 
TCAGGAACAA ATCCTAGAGT TACCCCTGCA TTGCCTCAAG GACCTGGGCA AGGGCCAAGA 
ATGGAGAGTG GAAGCAGGTC AAACTCTGGA GCTGGACTTC CAGGCCCAAA AGGACAGATG 
CCTGGCAAAG ACAGCTTTCC TTTCCCATTT CCTGATGATT TTCCATTTCC AGGAGATATG 
GACAATTTCT CCTTCCCTGA TATGAATAAA CTAGATGCAA TGTTTTCAGG TGGTTTTAAA 
TCTGATGAGT TTCCATTTTC CTTCCCTGAT TTTCCGATGC CAGGTGGTTT AATGGGACCC 
GACTTTCCAT TACCTGAAGG ACTGAAACCC GAAGACATGA CCAAAGATAT GGAAGAATTC 
ATGAAACAGA TGGAATCTGA ACTAGGCAAT GTTTCTGCAA AATTTCAACC AACCCCAAGG 
GGAAGCAACA TAGCCGATCA GGATAAACAG CAAGCTTTAA ATGCCAAAGC ATCAAGAGGA 
CCTGTAGACA TAGAGAGGGG TCCACAAGGA ATGAACAGAG ATTCTGCTGG GATGGGAAGA 
CACCCAATGG GAATGGATAG AGGTCCTCAA GGTATGGAGA GACACTCACA AGAATTCAAT 
AGAGGTCTAT CAGGAATAGA CAGAGGCCCA AGAGAAAGTC TTCAAGGGAG GGAAAGGGTC 
CCACCAGGAA TGGATAGAGG ACAACAAGGA ATGGATAGAT GCCCTCCCGG AATGGATAGA 
AGAAGACCAG GCATGGACGG AGGCCCTCTT GGAATGGATA GAGGACAACC AGGAATGGAT 
AGAGGACAAC CAGGAATGGA CAGAGGCCCT CTTGGAATGG ATAGAGGACA ACCAGAAATG 
GACAGAGGCC CTCTTGGAAT GGATAGAGGA CAACCAGGAA TTGACGGAGG CCCTCTTGGA 
ATGAATAGAG GACAACCAGG AATGGATAGA GGCACTCCTG GAATGGATAG AGGTCCCCCT 
GGGATGGATA GGGGACCAAC CGGAATGGAT AGAGGACAAC CAAGCTTGGA TAGAGGTCCT 
CCTGGAATGG ATAGAGGACA GCAAGGAATG GATAGAGGTC AACCAGGAAT GGATAGAGGA 
CAACCAGGAA TGGATAGAGG ACAACCAGGA ATGGATAGAG GACAAACAGG AATGGATAGA 
GGTCCTCCTG GAATGGATAG AGGACAGCAA GGAATGGAGA GAGTTCCTCC TGGAACAGAC 
AAAAGACTAC CCGGAATGGA TAGAGGCCCT CCTGGAATGG ATGGAGGCCC TCCCAGACTG 
GATAGAGGAC AACCAGGCAT GGATAAAGGC CCTCCTGGAA TGAATAGAGG CCCTCCCGGA 
ATGGATGGAG GCCCTCCCGG AATGGATAGA GGACATCCAG GCATGGATAA AGGCCCTCCT 
GGAATGGATA GAGGCCCTCC CGGAATGGAG AGAGGCCCAC CTGGACTGGA TAGAGGACAT 
CCAGGCATGG ATAAAGGCCC TCCTGGAATG GAGAGTGGAC ATCCAGGCAT AGATAAAGGC 
CCTCCTGGAA TGGATAGAGG TACAGCAGGA AGGAATAGAG GCCAATCAGA AATGGATAGA 
GGCTCATCAA GAAATGAGGG AGATGCACCA AGAACTAATA AAGTTCAACT AGGGATCGGG 
GGAATTCTTG CAGGACTTAA AGATTCATCA GGTATGGATA ATGATCATAG AATGATTGAC 
AGAGGCCCAC CAGGAATTGA CAGTGGCCCT CCAGGAGCAG ACAGTGGCCC ACCAGGAGTA 
GACAGTGGTG CATTGAGAAC CGATAGAAGT CAACTAGCTT TAGGGAACGT TCTTGCTGGG 
TTGAGGGCAG CCCCACCAGG AATGGATAGA GGCCCACCAG GAATAGACGG TGGCCCACCA 
GGAATAGACA GTGGCCCACC AGGAATAGAA ATAAACGCCC CAGGTCGTAC TAAAAGTCAA 
CTAGGTTTAG GGAGCATTCT TGCTGCACTG AAGACAGGTC CACCAGGAAT GGATACGGGT 
CCACCTGGTG TGGAGATGAG ACCTAGTGGC ATGGACAAGG GACCCCCGGG AATAGACATA 
GCCCCTCCAG TTATGGATAT GGGTCCTCCA GGAACGGACA GAGGACCTCC ATCTAATTAC 
ATTAAACCTC CAGGGATGGA CAACTCACCT CCAGTTATGG GGACATTGCC CATGAGTTTG 
ACCAGGGGTC CTCCTGGTCC ACCTGGAGTT GATGTTGGAC CTCCTGGTGT CGATGTTGGA 
CCTCCTGGTG TGGATATTGA TGCAATTAGT ATGCATAGCC ATTATTTGGG AGCTGGAAAG 
CCTCGCCCTC CATTTGATCA AAGGCAATTG GATATTGGTC CACCAGGTGT AGATACCTTT 
CCCCCTGGAA TTCCACGTTA TCCTTTTGGT TCAACACAAG GTCCAGCAGG ATTTGGAAAC 
TCATCTTCAA TTCAAGATAG CAGCTTTGAA GAGCCAATGC CTCCTGGTAT GGAGCCTATT 
AGTAAACCCC TTGCTTTTCC CAGAGATCTT GCACCTCCTG GGATGGATGA GACATTTCCA 
CCAGGGATGG AACCAGAACA GGAAAGCCAG CCAAGCACAA AAAGTAAACA GCCGCAACCA 
CCGGAAGACA ATATAAGTGA ATTTGAAAAG GGGCAAAACA AGCCACTGTT TCCCCCACCA 
TCCAAAGGTT TGACTCAACA TTCCTATTCA GTTGGACAAT CCATACCAAT TACAGGTATG 
GTGAAAGATT CTTTTGGTGC CAGCTCCTTT GTTTCAACAA AACAGACATT AGCTCCAAGC 
CCACTTGCTG GGTATGAGTC ATTAGAATCG GTAAAGGCCA AGAAAGAAGA AAAGAAAAAA 
GATGAATCAA AGTCAGAGGA TAAAAAAAAA GGAAAAGGGA AAAAGGGAAA TATGAACATG 
CCTACAATCA TCAAATTTGG GGATTCTAAA ATTGATAAAG ATTCTGATGA TCCTGTCAAA 
GTTGAAAAGG ACGAGACATC TACTGATGAA AAAGCAGCAA AGGAGGCTGC AAAAGCTAAG 
GAGTTTAAAA AAGAAAATTC ACCTTCTGAG GAGGTTTCAA AGAGAGTAAC TGTGCGTAGC 
ACCCTACCAT TGATCACAGA TTCTACAACC AAGTCTATGA TACCTTCATC AATTGGTGAG 
CTATTCAAGA AGAACTCTAC AACACCTCCA GCATTCAACA AAGATGTAAA GACCTCACTT 
AGGAGCTACA GGAAAGCAGC AGAAGATGAT GAGGAAGAGG AAGAAGCGCC TCACTCTAAA 
AAGATGAATA GGAAGGATTC TAGCGATAGA GATGATAGGG GACGGGATGA CACACTCCGC 
ACTACACTCC CATCCCCCCC ACGGTCATAT CGGTCACCTG ATCGAAGGTC TAGGAGATCT 
CCTGAAAGAA GGAGATCTCG TTCTCCAGAC AAAAGAAAGC GTAGATCTCG GTCTAGATCG 
ATCTCACAGG ACCGCAGGAA ACGGAGGCGG GATTCTCCAC CCCGTAAGAG AAACTCATAC 
TCTCCGAGCA AAAGGAGGTC CCCCTCTAAA AGTAAAAATT CACGATCCCC AAGCAGAGAC 
AAGGGCAATA GATCCCCGGA TCGAAGAGAT CAGAGAGATC GAAGAGATCA GAGAGATCAA 
AGAGATCAGA GAGATCGAAG AGATGGGAGA GATGACAGAT ACAGAGAAAA GGACAGGAGG 
GACAGGTATC CTGACAAAGA ATATGAGAAA GATTCTGATA GGAGAGGTGG GAAGCCAAGT 
ACAGAAGCCT TTGACAAGTT GCGAGAGCTG ATTTATGATA CCTTGGTGCG CCATCCTGAT 
GAAAAGCCAA GGGATTCTCC ACATAGCGAT AGGCGACACC CGGACGATAG ATCTCCTCAG 
CGACCAAGAA GGGAGAACTA TTACCCGGAA GCTGAGAGGC CAAGAATGCG ATACCCTCCA 
CCACGTAGAG ACTATGAAAG AGATCCTTTG AGGGATCGGT TAAGAAGCCT TCCAAGGGAT 
TCACCAAGGG ATCTTCCATT GCCAGCTCAA AGGGAGCCTC GTAGGGATTT TGATAGAGAT 
TCCTCTCAAG ACAGATCTAT GGAGAGGGCT CATTCCCCTC CGGTGACGGA AATAACAGCA 
CCTATTGTCT TTGACTATGG GCATGCAGAG TCCACCAGGC CTGCTTATGA CATAGAGACA 
AGAGATCCCA TGGGAAGCCT GCTCAGCAAT GCAGTTATTG TAGACGTTGT TGATTATAGT 
CATGGTCAGA ATCAGGGACC AGATGCTAGA GCAATTAGCG GATCAGAACT TCTTCAAGAG 
CGGGAGTCAT CGTTCCCTTC AGAATTTCAA ATACATAAAG AGATTCAACC CGCAAAGAGA 
GATGATACCA GTATGCAACA ATCACCAGCT CCACCACATC GTGAACCTTC ACCTCAGCCT 
CCACCTCAGC CACCCATGCA GCAATCACTT CCCCAGCAAA CTCAGCAGCA AGAACTTCCC 
TACAGAGAAC AGCACCAGCA ATTCTCTCAG CAGTACCTGC CAGATGATCT GACACAACAG 
TTTGATCCAT ACAGTGGCCA GCAACAGATT GGCTATTACC AGCAACCAGA ATCACAGATG 
GCATTCCAAC AACAACAACA CCTGCAAGCA TTCCAGCAAA TGGGCCAGTT CCCCCAGCAG 
GCTCAGTACA ACACCCAGCA ACAGCAGCAA CACTATACGG AACAGTTCGA GCAGCAGCAG 
CAGCAGCCGC CGCCACCACC ACCTCCACCA TCTGAAGATT TGCGGTCAAG TGCCTACCGC 
AAGTCCCCTC CCCCGCCGCC ACCTGATACT CCTGCCCCAC CGAAGGATGA TTCCTTTCAG 
CAATCTTGGG CAGCTGCACC TCCCCCACCT CCATCTGACT CTTACACCAG TACCAGACAA 
TCTGGAGGTC AAGACCAAGA CAGATCCAGG GAGAGAGGGG ATGACAGGCA ACGTGGAAGG 
GGTGATAAGC AATCTGGAGG TCAAGACCAA GACAGATCCA GGGAGAGAGG AGATGACAGG 
CAACGTGGAA GGGGGGATAA ATACCAGAGT GGCCCTGCCA GGCTAGTGGA GGACTACAGG 
GACTCCCCTG GACAGAAGGA CATGTTCACC AACTCACCCC AGCAAACCCC TCCTCCCCCT 
TCCAAGCCCA AGAAGAGGGA AGTGGTGGCA GTCACGGACC TCCTAGACCC GCCCAACAGG 
GAGAAACGCC CAGCCAAGAT CTGTATGATC CTGCGTGGCC CTCCTGGAAG CGGCAAGAGT 
TACATTGCAA AGCTTATCAA GGACAGAGAA ATACAAAACC GAGGTCCTAG TCCTCGCATG 
CTGTGTCTGG ATGACTACTT TATGAGTGAG AAGGAGAAGA CGGAAAAGGA CGCTGAAACT 
GGCAAGATTG TCAAGAAGAG GTTTCAGGAA TACGAACATG AGGACAGCAT GGAGGCTATT 
TACCATGTCA GTTTACTCAA GACATTCAAG AAGACCATCG AAGACGGTTT CTTTGACTTC 
ATCATCGTGG ATGCCGTCAA CGACAAAATC TCAAAGATTG AACCGTTCTG GAGCTATGCA 
AAGATGAAAG GATTTGAGGT GTATGTAGCT GAGTTACTTG TTGAATTAGG CTTGTGCATT 
CAGCGAGACA TCCACAATAG AGGTGAAACA GGAATCAGAA AGATCATAGA ACACTGGGAG 
TTTGCCCCAC GGAAATACAT CAAGCTAGAT GTGGTGGCCC TCCTGCAAGA TGCTGCCATT 
CCAGAGGTTG AGATGGAGGA ACTTGATGAA GACGCCGGAC CTTCCATGGA GGAACTGATG 
AACCAACAGC AGGAAGCCTT GGAAGAACTA GAGGATCAGG AAGAGACACT GGCTTTGACG 
AAGAGTAAGT GGGATTCTGC TACCGAAGAG AAACTAGATG CCCTTGATGG TATCAAGAAG 
TATAAGAAGA AGGAGAGCTA CACGTCTATG GAGGACTTCC TATCATGTGG AGATGAGTTT 
GACACAGTCT CCAAACCCAT ACTCCCAGGA CAGAAGAGAG TACGCTGGGC CGACATCGAG 
GAACGCCAAA ACCAAGAACG TATGCGTGCA GTAGGTTTTG TCGTTGGGCA GACGGCCACT 
GACTGGGCCA AGATCACTGA CGACAATTAT GCAGACAAGG CTCTCAATCG CACCAAGTAC 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030855690.1
CDS	124..7389
Translation	MFNNWNRPGGPGPGGPGERPPMMPGNGMPNRPPFYGPGSGPGAGGRPMHMSGPPGPGPGSHHMGMRMDSMNPRGPMSGPGHWHGPGMNMMGPGGPGPRRPPPPMTMSMMHGPGPGGPRPRMGMPIPEPVRVLVNQAQAADTAVPQPPQNEDVKLRGLQDQALHWQATAPVRKEEETVQKQAAAQDQWQSYQQQMQSYQQQYQQWYQSQETTLMDVKLKDLQEKKATCEKQLTDWKNQFDNWVEQHKKHPNKDQFDQYQNQWKTWQTQMESMVNSLDMQIKIKETSLQKNSSKNASTTQTPASVGSGVGTVTTQGANVAMPSGGHGMVPSSGAGMGQGSSIGSIPSSQSVATVPSGSGMGAGPRGLGVGPMPTGSGMGPGPSGTNPRVTPALPQGPGQGPRMESGSRSNSGAGLPGPKGQMPGKDSFPFPFPDDFPFPGDMDNFSFPDMNKLDAMFSGGFKSDEFPFSFPDFPMPGGLMGPDFPLPEGLKPEDMTKDMEEFMKQMESELGNVSAKFQPTPRGSNIADQDKQQALNAKASRGPVDIERGPQGMNRDSAGMGRHPMGMDRGPQGMERHSQEFNRGLSGIDRGPRESLQGRERVPPGMDRGQQGMDRCPPGMDRRRPGMDGGPLGMDRGQPGMDRGQPGMDRGPLGMDRGQPEMDRGPLGMDRGQPGIDGGPLGMNRGQPGMDRGTPGMDRGPPGMDRGPTGMDRGQPSLDRGPPGMDRGQQGMDRGQPGMDRGQPGMDRGQPGMDRGQTGMDRGPPGMDRGQQGMERVPPGTDKRLPGMDRGPPGMDGGPPRLDRGQPGMDKGPPGMNRGPPGMDGGPPGMDRGHPGMDKGPPGMDRGPPGMERGPPGLDRGHPGMDKGPPGMESGHPGIDKGPPGMDRGTAGRNRGQSEMDRGSSRNEGDAPRTNKVQLGIGGILAGLKDSSGMDNDHRMIDRGPPGIDSGPPGADSGPPGVDSGALRTDRSQLALGNVLAGLRAAPPGMDRGPPGIDGGPPGIDSGPPGIEINAPGRTKSQLGLGSILAALKTGPPGMDTGPPGVEMRPSGMDKGPPGIDIAPPVMDMGPPGTDRGPPSNYIKPPGMDNSPPVMGTLPMSLTRGPPGPPGVDVGPPGVDVGPPGVDIDAISMHSHYLGAGKPRPPFDQRQLDIGPPGVDTFPPGIPRYPFGSTQGPAGFGNSSSIQDSSFEEPMPPGMEPISKPLAFPRDLAPPGMDETFPPGMEPEQESQPSTKSKQPQPPEDNISEFEKGQNKPLFPPPSKGLTQHSYSVGQSIPITGMVKDSFGASSFVSTKQTLAPSPLAGYESLESVKAKKEEKKKDESKSEDKKKGKGKKGNMNMPTIIKFGDSKIDKDSDDPVKVEKDETSTDEKAAKEAAKAKEFKKENSPSEEVSKRVTVRSTLPLITDSTTKSMIPSSIGELFKKNSTTPPAFNKDVKTSLRSYRKAAEDDEEEEEAPHSKKMNRKDSSDRDDRGRDDTLRTTLPSPPRSYRSPDRRSRRSPERRRSRSPDKRKRRSRSRSISQDRRKRRRDSPPRKRNSYSPSKRRSPSKSKNSRSPSRDKGNRSPDRRDQRDRRDQRDQRDQRDRRDGRDDRYREKDRRDRYPDKEYEKDSDRRGGKPSTEAFDKLRELIYDTLVRHPDEKPRDSPHSDRRHPDDRSPQRPRRENYYPEAERPRMRYPPPRRDYERDPLRDRLRSLPRDSPRDLPLPAQREPRRDFDRDSSQDRSMERAHSPPVTEITAPIVFDYGHAESTRPAYDIETRDPMGSLLSNAVIVDVVDYSHGQNQGPDARAISGSELLQERESSFPSEFQIHKEIQPAKRDDTSMQQSPAPPHREPSPQPPPQPPMQQSLPQQTQQQELPYREQHQQFSQQYLPDDLTQQFDPYSGQQQIGYYQQPESQMAFQQQQHLQAFQQMGQFPQQAQYNTQQQQQHYTEQFEQQQQQPPPPPPPPSEDLRSSAYRKSPPPPPPDTPAPPKDDSFQQSWAAAPPPPPSDSYTSTRQSGGQDQDRSRERGDDRQRGRGDKQSGGQDQDRSRERGDDRQRGRGDKYQSGPARLVEDYRDSPGQKDMFTNSPQQTPPPPSKPKKREVVAVTDLLDPPNREKRPAKICMILRGPPGSGKSYIAKLIKDREIQNRGPSPRMLCLDDYFMSEKEKTEKDAETGKIVKKRFQEYEHEDSMEAIYHVSLLKTFKKTIEDGFFDFIIVDAVNDKISKIEPFWSYAKMKGFEVYVAELLVELGLCIQRDIHNRGETGIRKIIEHWEFAPRKYIKLDVVALLQDAAIPEVEMEELDEDAGPSMEELMNQQQEALEELEDQEETLALTKSKWDSATEEKLDALDGIKKYKKKESYTSMEDFLSCGDEFDTVSKPILPGQKRVRWADIEERQNQERMRAVGFVVGQTATDWAKITDDNYADKALNRTKYI

Target ORF information:

RefSeq Version	XM_030999830.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030999830.1

ORF Insert Sequence:

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GACCAGGTAT GAATATGATG GGACCAGGTG GACCAGGACC AAGAAGACCA 
CCTCCACCGA TGACGATGTC GATGATGCAT GGTCCTGGTC CAGGTGGCCC AAGGCCAAGA 
ATGGGTATGC CCATCCCAGA GCCTGTTCGG GTGTTAGTCA ATCAAGCGCA AGCCGCAGAT 
ACTGCAGTCC CACAACCGCC TCAGAATGAA GATGTGAAGT TGAGAGGGCT TCAGGATCAG 
GCCTTACATT GGCAGGCAAC GGCCCCGGTT CGAAAGGAGG AAGAAACTGT ACAAAAACAG 
GCAGCTGCTC AAGACCAGTG GCAGAGCTAC CAGCAACAGA TGCAATCATA TCAGCAACAG 
TATCAACAAT GGTACCAAAG TCAAGAGACT ACCTTGATGG ATGTTAAATT GAAAGACTTG 
CAGGAAAAGA AGGCTACCTG TGAAAAGCAG CTCACAGACT GGAAAAATCA GTTTGATAAT 
TGGGTAGAAC AGCATAAGAA ACATCCAAAC AAGGACCAGT TTGATCAATA TCAAAATCAA 
TGGAAAACAT GGCAAACACA GATGGAGAGC ATGGTGAATT CTTTGGACAT GCAAATCAAA 
ATAAAAGAGA CATCCCTTCA GAAGAACTCC TCTAAAAATG CTTCAACAAC CCAGACACCT 
GCTTCTGTCG GCAGTGGTGT AGGAACAGTT ACCACACAAG GTGCAAATGT AGCAATGCCA 
TCAGGTGGGC ATGGAATGGT TCCATCTAGT GGTGCTGGAA TGGGACAAGG GTCATCAATT 
GGTTCTATAC CAAGCAGTCA GAGTGTAGCC ACAGTTCCAA GTGGTTCTGG GATGGGAGCA 
GGACCCAGAG GACTTGGAGT AGGACCAATG CCAACTGGGA GTGGAATGGG ACCAGGTCCC 
TCAGGAACAA ATCCTAGAGT TACCCCTGCA TTGCCTCAAG GACCTGGGCA AGGGCCAAGA 
ATGGAGAGTG GAAGCAGGTC AAACTCTGGA GCTGGACTTC CAGGCCCAAA AGGACAGATG 
CCTGGCAAAG ACAGCTTTCC TTTCCCATTT CCTGATGATT TTCCATTTCC AGGAGATATG 
GACAATTTCT CCTTCCCTGA TATGAATAAA CTAGATGCAA TGTTTTCAGG TGGTTTTAAA 
TCTGATGAGT TTCCATTTTC CTTCCCTGAT TTTCCGATGC CAGGTGGTTT AATGGGACCC 
GACTTTCCAT TACCTGAAGG ACTGAAACCC GAAGACATGA CCAAAGATAT GGAAGAATTC 
ATGAAACAGA TGGAATCTGA ACTAGGCAAT GTTTCTGCAA AATTTCAACC AACCCCAAGG 
GGAAGCAACA TAGCCGATCA GGATAAACAG CAAGCTTTAA ATGCCAAAGC ATCAAGAGGA 
CCTGTAGACA TAGAGAGGGG TCCACAAGGA ATGAACAGAG ATTCTGCTGG GATGGGAAGA 
CACCCAATGG GAATGGATAG AGGTCCTCAA GGTATGGAGA GACACTCACA AGAATTCAAT 
AGAGGTCTAT CAGGAATAGA CAGAGGCCCA AGAGAAAGTC TTCAAGGGAG GGAAAGGGTC 
CCACCAGGAA TGGATAGAGG ACAACAAGGA ATGGATAGAT GCCCTCCCGG AATGGATAGA 
AGAAGACCAG GCATGGACGG AGGCCCTCTT GGAATGGATA GAGGACAACC AGGAATGGAT 
AGAGGACAAC CAGGAATGGA CAGAGGCCCT CTTGGAATGG ATAGAGGACA ACCAGAAATG 
GACAGAGGCC CTCTTGGAAT GGATAGAGGA CAACCAGGAA TTGACGGAGG CCCTCTTGGA 
ATGAATAGAG GACAACCAGG AATGGATAGA GGCACTCCTG GAATGGATAG AGGTCCCCCT 
GGGATGGATA GGGGACCAAC CGGAATGGAT AGAGGACAAC CAAGCTTGGA TAGAGGTCCT 
CCTGGAATGG ATAGAGGACA GCAAGGAATG GATAGAGGTC AACCAGGAAT GGATAGAGGA 
CAACCAGGAA TGGATAGAGG ACAACCAGGA ATGGATAGAG GACAAACAGG AATGGATAGA 
GGTCCTCCTG GAATGGATAG AGGACAGCAA GGAATGGAGA GAGTTCCTCC TGGAACAGAC 
AAAAGACTAC CCGGAATGGA TAGAGGCCCT CCTGGAATGG ATGGAGGCCC TCCCAGACTG 
GATAGAGGAC AACCAGGCAT GGATAAAGGC CCTCCTGGAA TGAATAGAGG CCCTCCCGGA 
ATGGATGGAG GCCCTCCCGG AATGGATAGA GGACATCCAG GCATGGATAA AGGCCCTCCT 
GGAATGGATA GAGGCCCTCC CGGAATGGAG AGAGGCCCAC CTGGACTGGA TAGAGGACAT 
CCAGGCATGG ATAAAGGCCC TCCTGGAATG GAGAGTGGAC ATCCAGGCAT AGATAAAGGC 
CCTCCTGGAA TGGATAGAGG TACAGCAGGA AGGAATAGAG GCCAATCAGA AATGGATAGA 
GGCTCATCAA GAAATGAGGG AGATGCACCA AGAACTAATA AAGTTCAACT AGGGATCGGG 
GGAATTCTTG CAGGACTTAA AGATTCATCA GGTATGGATA ATGATCATAG AATGATTGAC 
AGAGGCCCAC CAGGAATTGA CAGTGGCCCT CCAGGAGCAG ACAGTGGCCC ACCAGGAGTA 
GACAGTGGTG CATTGAGAAC CGATAGAAGT CAACTAGCTT TAGGGAACGT TCTTGCTGGG 
TTGAGGGCAG CCCCACCAGG AATGGATAGA GGCCCACCAG GAATAGACGG TGGCCCACCA 
GGAATAGACA GTGGCCCACC AGGAATAGAA ATAAACGCCC CAGGTCGTAC TAAAAGTCAA 
CTAGGTTTAG GGAGCATTCT TGCTGCACTG AAGACAGGTC CACCAGGAAT GGATACGGGT 
CCACCTGGTG TGGAGATGAG ACCTAGTGGC ATGGACAAGG GACCCCCGGG AATAGACATA 
GCCCCTCCAG TTATGGATAT GGGTCCTCCA GGAACGGACA GAGGACCTCC ATCTAATTAC 
ATTAAACCTC CAGGGATGGA CAACTCACCT CCAGTTATGG GGACATTGCC CATGAGTTTG 
ACCAGGGGTC CTCCTGGTCC ACCTGGAGTT GATGTTGGAC CTCCTGGTGT CGATGTTGGA 
CCTCCTGGTG TGGATATTGA TGCAATTAGT ATGCATAGCC ATTATTTGGG AGCTGGAAAG 
CCTCGCCCTC CATTTGATCA AAGGCAATTG GATATTGGTC CACCAGGTGT AGATACCTTT 
CCCCCTGGAA TTCCACGTTA TCCTTTTGGT TCAACACAAG GTCCAGCAGG ATTTGGAAAC 
TCATCTTCAA TTCAAGATAG CAGCTTTGAA GAGCCAATGC CTCCTGGTAT GGAGCCTATT 
AGTAAACCCC TTGCTTTTCC CAGAGATCTT GCACCTCCTG GGATGGATGA GACATTTCCA 
CCAGGGATGG AACCAGAACA GGAAAGCCAG CCAAGCACAA AAAGTAAACA GCCGCAACCA 
CCGGAAGACA ATATAAGTGA ATTTGAAAAG GGGCAAAACA AGCCACTGTT TCCCCCACCA 
TCCAAAGGTT TGACTCAACA TTCCTATTCA GTTGGACAAT CCATACCAAT TACAGGTATG 
GTGAAAGATT CTTTTGGTGC CAGCTCCTTT GTTTCAACAA AACAGACATT AGCTCCAAGC 
CCACTTGCTG GGTATGAGTC ATTAGAATCG GTAAAGGCCA AGAAAGAAGA AAAGAAAAAA 
GATGAATCAA AGTCAGAGGA TAAAAAAAAA GGAAAAGGGA AAAAGGGAAA TATGAACATG 
CCTACAATCA TCAAATTTGG GGATTCTAAA ATTGATAAAG ATTCTGATGA TCCTGTCAAA 
GTTGAAAAGG ACGAGACATC TACTGATGAA AAAGCAGCAA AGGAGGCTGC AAAAGCTAAG 
GAGTTTAAAA AAGAAAATTC ACCTTCTGAG GAGGTTTCAA AGAGAGTAAC TGTGCGTAGC 
ACCCTACCAT TGATCACAGA TTCTACAACC AAGTCTATGA TACCTTCATC AATTGGTGAG 
CTATTCAAGA AGAACTCTAC AACACCTCCA GCATTCAACA AAGATGTAAA GACCTCACTT 
AGGAGCTACA GGAAAGCAGC AGAAGATGAT GAGGAAGAGG AAGAAGCGCC TCACTCTAAA 
AAGATGAATA GGAAGGATTC TAGCGATAGA GATGATAGGG GACGGGATGA CACACTCCGC 
ACTACACTCC CATCCCCCCC ACGGTCATAT CGGTCACCTG ATCGAAGGTC TAGGAGATCT 
CCTGAAAGAA GGAGATCTCG TTCTCCAGAC AAAAGAAAGC GTAGATCTCG GTCTAGATCG 
ATCTCACAGG ACCGCAGGAA ACGGAGGCGG GATTCTCCAC CCCGTAAGAG AAACTCATAC 
TCTCCGAGCA AAAGGAGGTC CCCCTCTAAA AGTAAAAATT CACGATCCCC AAGCAGAGAC 
AAGGGCAATA GATCCCCGGA TCGAAGAGAT CAGAGAGATC GAAGAGATCA GAGAGATCAA 
AGAGATCAGA GAGATCGAAG AGATGGGAGA GATGACAGAT ACAGAGAAAA GGACAGGAGG 
GACAGGTATC CTGACAAAGA ATATGAGAAA GATTCTGATA GGAGAGGTGG GAAGCCAAGT 
ACAGAAGCCT TTGACAAGTT GCGAGAGCTG ATTTATGATA CCTTGGTGCG CCATCCTGAT 
GAAAAGCCAA GGGATTCTCC ACATAGCGAT AGGCGACACC CGGACGATAG ATCTCCTCAG 
CGACCAAGAA GGGAGAACTA TTACCCGGAA GCTGAGAGGC CAAGAATGCG ATACCCTCCA 
CCACGTAGAG ACTATGAAAG AGATCCTTTG AGGGATCGGT TAAGAAGCCT TCCAAGGGAT 
TCACCAAGGG ATCTTCCATT GCCAGCTCAA AGGGAGCCTC GTAGGGATTT TGATAGAGAT 
TCCTCTCAAG ACAGATCTAT GGAGAGGGCT CATTCCCCTC CGGTGACGGA AATAACAGCA 
CCTATTGTCT TTGACTATGG GCATGCAGAG TCCACCAGGC CTGCTTATGA CATAGAGACA 
AGAGATCCCA TGGGAAGCCT GCTCAGCAAT GCAGTTATTG TAGACGTTGT TGATTATAGT 
CATGGTCAGA ATCAGGGACC AGATGCTAGA GCAATTAGCG GATCAGAACT TCTTCAAGAG 
CGGGAGTCAT CGTTCCCTTC AGAATTTCAA ATACATAAAG AGATTCAACC CGCAAAGAGA 
GATGATACCA GTATGCAACA ATCACCAGCT CCACCACATC GTGAACCTTC ACCTCAGCCT 
CCACCTCAGC CACCCATGCA GCAATCACTT CCCCAGCAAA CTCAGCAGCA AGAACTTCCC 
TACAGAGAAC AGCACCAGCA ATTCTCTCAG CAGTACCTGC CAGATGATCT GACACAACAG 
TTTGATCCAT ACAGTGGCCA GCAACAGATT GGCTATTACC AGCAACCAGA ATCACAGATG 
GCATTCCAAC AACAACAACA CCTGCAAGCA TTCCAGCAAA TGGGCCAGTT CCCCCAGCAG 
GCTCAGTACA ACACCCAGCA ACAGCAGCAA CACTATACGG AACAGTTCGA GCAGCAGCAG 
CAGCAGCCGC CGCCACCACC ACCTCCACCA TCTGAAGATT TGCGGTCAAG TGCCTACCGC 
AAGTCCCCTC CCCCGCCGCC ACCTGATACT CCTGCCCCAC CGAAGGATGA TTCCTTTCAG 
CAATCTTGGG CAGCTGCACC TCCCCCACCT CCATCTGACT CTTACACCAG TACCAGACAA 
TCTGGAGGTC AAGACCAAGA CAGATCCAGG GAGAGAGGGG ATGACAGGCA ACGTGGAAGG 
GGTGATAAGC AATCTGGAGG TCAAGACCAA GACAGATCCA GGGAGAGAGG AGATGACAGG 
CAACGTGGAA GGGGGGATAA ATACCAGAGT GGCCCTGCCA GGCTAGTGGA GGACTACAGG 
GACTCCCCTG GACAGAAGGA CATGTTCACC AACTCACCCC AGCAAACCCC TCCTCCCCCT 
TCCAAGCCCA AGAAGAGGGA AGTGGTGGCA GTCACGGACC TCCTAGACCC GCCCAACAGG 
GAGAAACGCC CAGCCAAGAT CTGTATGATC CTGCGTGGCC CTCCTGGAAG CGGCAAGAGT 
TACATTGCAA AGCTTATCAA GGACAGAGAA ATACAAAACC GAGGTCCTAG TCCTCGCATG 
CTGTGTCTGG ATGACTACTT TATGAGTGAG AAGGAGAAGA CGGAAAAGGA CGCTGAAACT 
GGCAAGATTG TCAAGAAGAG GTTTCAGGAA TACGAACATG AGGACAGCAT GGAGGCTATT 
TACCATGTCA GTTTACTCAA GACATTCAAG AAGACCATCG AAGACGGTTT CTTTGACTTC 
ATCATCGTGG ATGCCGTCAA CGACAAAATC TCAAAGATTG AACCGTTCTG GAGCTATGCA 
AAGATGAAAG GATTTGAGGT GTATGTAGCT GAGTTACTTG TTGAATTAGG CTTGTGCATT 
CAGCGAGACA TCCACAATAG AGGTGAAACA GGAATCAGAA AGATCATAGA ACACTGGGAG 
TTTGCCCCAC GGAAATACAT CAAGCTAGAT GTGGTGGCCC TCCTGCAAGA TGCTGCCATT 
CCAGAGGTTG AGATGGAGGA ACTTGATGAA GACGCCGGAC CTTCCATGGA GGAACTGATG 
AACCAACAGC AGGAAGCCTT GGAAGAACTA GAGGATCAGG AAGAGACACT GGCTTTGACG 
AAGAGTAAGT GGGATTCTGC TACCGAAGAG AAACTAGATG CCCTTGATGG TATCAAGAAG 
TATAAGAAGA AGGAGAGCTA CACGTCTATG GAGGACTTCC TATCATGTGG AGATGAGTTT 
GACACAGTCT CCAAACCCAT ACTCCCAGGA CAGAAGAGAG TACGCTGGGC CGACATCGAG 
GAACGCCAAA ACCAAGAACG TATGCGTGCA GTAGGTTTTG TCGTTGGGCA GACGGCCACT 
GACTGGGCCA AGATCACTGA CGACAATTAT GCAGACAAGG CTCTCAATCG CACCAAGTAC 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC581149 ( XM_030999831.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030855691.1 Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X4, mRNA.

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CloneID	OSd65791
Clone ID Related Accession (Same CDS sequence)	XM_030999831.1
Accession Version	XM_030999831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	YLP motif-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GACCAGGTAT GAATATGATG GGACCAGGTG GACCAGGACC AAGAAGACCA 
CCTCCACCGA TGACGATGTC GATGATGCAT GGTCCTGGTC CAGGTGGCCC AAGGCCAAGA 
ATGGGTATGC CCATCCCAGA GCCTGTTCGG GTGTTAGTCA ATCAAGCGCA AGCCGCAGAT 
ACTGCAGTCC CACAACCGCC TCAGAATGAA GATGTGAAGT TGAGAGGGCT TCAGGATCAG 
GCCTTACATT GGCAGGCAAC GGCCCCGGTT CGAAAGGAGG AAGAAACTGT ACAAAAACAG 
GCAGCTGCTC AAGACCAGTG GCAGAGCTAC CAGCAACAGA TGCAATCATA TCAGCAACAG 
TATCAACAAT GGTACCAAAG TCAAGAGACT ACCTTGATGG ATGTTAAATT GAAAGACTTG 
CAGGAAAAGA AGGCTACCTG TGAAAAGCAG CTCACAGACT GGAAAAATCA GTTTGATAAT 
TGGGTAGAAC AGCATAAGAA ACATCCAAAC AAGGACCAGT TTGATCAATA TCAAAATCAA 
TGGAAAACAT GGCAAACACA GATGGAGAGC ATGGTGAATT CTTTGGACAT GCAAATCAAA 
ATAAAAGAGA CATCCCTTCA GAAGAACTCC TCTAAAAATG CTTCAACAAC CCAGACACCT 
GCTTCTGTCG GCAGTGGTGT AGGAACAGTT ACCACACAAG GTGCAAATGT AGCAATGCCA 
TCAGGTGGGC ATGGAATGGT TCCATCTAGT GGTGCTGGAA TGGGACAAGG GTCATCAATT 
GGTTCTATAC CAAGCAGTCA GAGTGTAGCC ACAGTTCCAA GTGGTTCTGG GATGGGAGCA 
GGACCCAGAG GACTTGGAGT AGGACCAATG CCAACTGGGA GTGGAATGGG ACCAGGTCCC 
TCAGGAACAA ATCCTAGAGT TACCCCTGCA TTGCCTCAAG GACCTGGGCA AGGGCCAAGA 
ATGGAGAGTG GAAGCAGGTC AAACTCTGGA GCTGGACTTC CAGGCCCAAA AGGACAGATG 
CCTGGCAAAG ACAGCTTTCC TTTCCCATTT CCTGATGATT TTCCATTTCC AGGAGATATG 
GACAATTTCT CCTTCCCTGA TATGAATAAA CTAGATGCAA TGTTTTCAGG TGGTTTTAAA 
TCTGATGAGT TTCCATTTTC CTTCCCTGAT TTTCCGATGC CAGGTGGTTT AATGGGACCC 
GACTTTCCAT TACCTGAAGG ACTGAAACCC GAAGACATGA CCAAAGATAT GGAAGAATTC 
ATGAAACAGA TGGAATCTGA ACTAGGCAAT GTTTCTGCAA AATTTCAACC AACCCCAAGG 
GGAAGCAACA TAGCCGATCA GGATAAACAG CAAGCTTTAA ATGCCAAAGC ATCAAGAGGA 
CCTGTAGACA TAGAGAGGGG TCCACAAGGA ATGAACAGAG ATTCTGCTGG GATGGGAAGA 
CACCCAATGG GAATGGATAG AGGTCCTCAA GGTATGGAGA GACACTCACA AGAATTCAAT 
AGAGGTCTAT CAGGAATAGA CAGAGGCCCA AGAGAAAGTC TTCAAGGGAG GGAAAGGGTC 
CCACCAGGAA TGGATAGAGG ACAACAAGGA ATGGATAGAT GCCCTCCCGG AATGGATAGA 
AGAAGACCAG GCATGGACGG AGGCCCTCTT GGAATGGATA GAGGACAACC AGGAATGGAT 
AGAGGACAAC CAGGAATGGA CAGAGGCACT CCTGGAATGG ATAGAGGTCC CCCTGGGATG 
GATAGGGGAC CAACCGGAAT GGATAGAGGA CAACCAAGCT TGGATAGAGG TCCTCCTGGA 
ATGGATAGAG GACAGCAAGG AATGGATAGA GGTCAACCAG GAATGGATAG AGGACAACCA 
GGAATGGATA GAGGACAACC AGGAATGGAT AGAGGTCCTC CTGGAATGGA TAGAGGACAG 
CAACGAATGG ATAGAGGACA GCAAGGAATG GATAGAGGTT ATCCTGGAAT GGATAGAGGA 
CAAACAGGAA TGGATAGAGG TCCTCCTGGA ATGGATAGAG GACAGCAAGG AATGGAGAGA 
GTTCCTCCTG GAACAGACAA AAGACTACCC GGAATGGATA GAGGCCCTCC TGGAATGGAT 
GGAGGCCCTC CCAGACTGGA TAGAGGACAA CCAGGCATGG ATAAAGGCCC TCCTGGAATG 
AATAGAGGCC CTCCCGGAAT GGATGGAGGC CCTCCCGGAA TGGATAGAGG ACATCCAGGC 
ATGGATAAAG GCCCTCCTGG AATGGATAGA GGCCCTCCCG GAATGGAGAG AGGCCCACCT 
GGACTGGATA GAGGACATCC AGGCATGGAT AAAGGCCCTC CTGGAATGGA GAGTGGACAT 
CCAGGCATAG ATAAAGGCCC TCCTGGAATG GATAGAGGTA CAGCAGGAAG GAATAGAGGC 
CAATCAGAAA TGGATAGAGG CTCATCAAGA AATGAGGGAG ATGCACCAAG AACTAATAAA 
GTTCAACTAG GGATCGGGGG AATTCTTGCA GGACTTAAAG ATTCATCAGG TATGGATAAT 
GATCATAGAA TGATTGACAG AGGCCCACCA GGAATTGACA GTGGCCCTCC AGGAGCAGAC 
AGTGGCCCAC CAGGAGTAGA CAGTGGTGCA TTGAGAACCG ATAGAAGTCA ACTAGCTTTA 
GGGAACGTTC TTGCTGGGTT GAGGGCAGCC CCACCAGGAA TGGATAGAGG CCCACCAGGA 
ATAGACGGTG GCCCACCAGG AATAGACAGT GGCCCACCAG GAATAGAAAT AAACGCCCCA 
GGTCGTACTA AAAGTCAACT AGGTTTAGGG AGCATTCTTG CTGCACTGAA GACAGGTCCA 
CCAGGAATGG ATACGGGTCC ACCTGGTGTG GAGATGAGAC CTAGTGGCAT GGACAAGGGA 
CCCCCGGGAA TAGACATAGC CCCTCCAGTT ATGGATATGG GTCCTCCAGG AACGGACAGA 
GGACCTCCAT CTAATTACAT TAAACCTCCA GGGATGGACA ACTCACCTCC AGTTATGGGG 
ACATTGCCCA TGAGTTTGAC CAGGGGTCCT CCTGGTCCAC CTGGAGTTGA TGTTGGACCT 
CCTGGTGTCG ATGTTGGACC TCCTGGTGTG GATATTGATG CAATTAGTAT GCATAGCCAT 
TATTTGGGAG CTGGAAAGCC TCGCCCTCCA TTTGATCAAA GGCAATTGGA TATTGGTCCA 
CCAGGTGTAG ATACCTTTCC CCCTGGAATT CCACGTTATC CTTTTGGTTC AACACAAGGT 
CCAGCAGGAT TTGGAAACTC ATCTTCAATT CAAGATAGCA GCTTTGAAGA GCCAATGCCT 
CCTGGTATGG AGCCTATTAG TAAACCCCTT GCTTTTCCCA GAGATCTTGC ACCTCCTGGG 
ATGGATGAGA CATTTCCACC AGGGATGGAA CCAGAACAGG AAAGCCAGCC AAGCACAAAA 
AGTAAACAGC CGCAACCACC GGAAGACAAT ATAAGTGAAT TTGAAAAGGG GCAAAACAAG 
CCACTGTTTC CCCCACCATC CAAAGGTTTG ACTCAACATT CCTATTCAGT TGGACAATCC 
ATACCAATTA CAGGTATGGT GAAAGATTCT TTTGGTGCCA GCTCCTTTGT TTCAACAAAA 
CAGACATTAG CTCCAAGCCC ACTTGCTGGG TATGAGTCAT TAGAATCGGT AAAGGCCAAG 
AAAGAAGAAA AGAAAAAAGA TGAATCAAAG TCAGAGGATA AAAAAAAAGG AAAAGGGAAA 
AAGGGAAATA TGAACATGCC TACAATCATC AAATTTGGGG ATTCTAAAAT TGATAAAGAT 
TCTGATGATC CTGTCAAAGT TGAAAAGGAC GAGACATCTA CTGATGAAAA AGCAGCAAAG 
GAGGCTGCAA AAGCTAAGGA GTTTAAAAAA GAAAATTCAC CTTCTGAGGA GGTTTCAAAG 
AGAGTAACTG TGCGTAGCAC CCTACCATTG ATCACAGATT CTACAACCAA GTCTATGATA 
CCTTCATCAA TTGGTGAGCT ATTCAAGAAG AACTCTACAA CACCTCCAGC ATTCAACAAA 
GATGTAAAGA CCTCACTTAG GAGCTACAGG AAAGCAGCAG AAGATGATGA GGAAGAGGAA 
GAAGCGCCTC ACTCTAAAAA GATGAATAGG AAGGATTCTA GCGATAGAGA TGATAGGGGA 
CGGGATGACA CACTCCGCAC TACACTCCCA TCCCCCCCAC GGTCATATCG GTCACCTGAT 
CGAAGGTCTA GGAGATCTCC TGAAAGAAGG AGATCTCGTT CTCCAGACAA AAGAAAGCGT 
AGATCTCGGT CTAGATCGAT CTCACAGGAC CGCAGGAAAC GGAGGCGGGA TTCTCCACCC 
CGTAAGAGAA ACTCATACTC TCCGAGCAAA AGGAGGTCCC CCTCTAAAAG TAAAAATTCA 
CGATCCCCAA GCAGAGACAA GGGCAATAGA TCCCCGGATC GAAGAGATCA GAGAGATCGA 
AGAGATCAGA GAGATCAAAG AGATCAGAGA GATCGAAGAG ATGGGAGAGA TGACAGATAC 
AGAGAAAAGG ACAGGAGGGA CAGGTATCCT GACAAAGAAT ATGAGAAAGA TTCTGATAGG 
AGAGGTGGGA AGCCAAGTAC AGAAGCCTTT GACAAGTTGC GAGAGCTGAT TTATGATACC 
TTGGTGCGCC ATCCTGATGA AAAGCCAAGG GATTCTCCAC ATAGCGATAG GCGACACCCG 
GACGATAGAT CTCCTCAGCG ACCAAGAAGG GAGAACTATT ACCCGGAAGC TGAGAGGCCA 
AGAATGCGAT ACCCTCCACC ACGTAGAGAC TATGAAAGAG ATCCTTTGAG GGATCGGTTA 
AGAAGCCTTC CAAGGGATTC ACCAAGGGAT CTTCCATTGC CAGCTCAAAG GGAGCCTCGT 
AGGGATTTTG ATAGAGATTC CTCTCAAGAC AGATCTATGG AGAGGGCTCA TTCCCCTCCG 
GTGACGGAAA TAACAGCACC TATTGTCTTT GACTATGGGC ATGCAGAGTC CACCAGGCCT 
GCTTATGACA TAGAGACAAG AGATCCCATG GGAAGCCTGC TCAGCAATGC AGTTATTGTA 
GACGTTGTTG ATTATAGTCA TGGTCAGAAT CAGGGACCAG ATGCTAGAGC AATTAGCGGA 
TCAGAACTTC TTCAAGAGCG GGAGTCATCG TTCCCTTCAG AATTTCAAAT ACATAAAGAG 
ATTCAACCCG CAAAGAGAGA TGATACCAGT ATGCAACAAT CACCAGCTCC ACCACATCGT 
GAACCTTCAC CTCAGCCTCC ACCTCAGCCA CCCATGCAGC AATCACTTCC CCAGCAAACT 
CAGCAGCAAG AACTTCCCTA CAGAGAACAG CACCAGCAAT TCTCTCAGCA GTACCTGCCA 
GATGATCTGA CACAACAGTT TGATCCATAC AGTGGCCAGC AACAGATTGG CTATTACCAG 
CAACCAGAAT CACAGATGGC ATTCCAACAA CAACAACACC TGCAAGCATT CCAGCAAATG 
GGCCAGTTCC CCCAGCAGGC TCAGTACAAC ACCCAGCAAC AGCAGCAACA CTATACGGAA 
CAGTTCGAGC AGCAGCAGCA GCAGCCGCCG CCACCACCAC CTCCACCATC TGAAGATTTG 
CGGTCAAGTG CCTACCGCAA GTCCCCTCCC CCGCCGCCAC CTGATACTCC TGCCCCACCG 
AAGGATGATT CCTTTCAGCA ATCTTGGGCA GCTGCACCTC CCCCACCTCC ATCTGACTCT 
TACACCAGTA CCAGACAATC TGGAGGTCAA GACCAAGACA GATCCAGGGA GAGAGGGGAT 
GACAGGCAAC GTGGAAGGGG TGATAAGCAA TCTGGAGGTC AAGACCAAGA CAGATCCAGG 
GAGAGAGGAG ATGACAGGCA ACGTGGAAGG GGGGATAAAT ACCAGAGTGG CCCTGCCAGG 
CTAGTGGAGG ACTACAGGGA CTCCCCTGGA CAGAAGGACA TGTTCACCAA CTCACCCCAG 
CAAACCCCTC CTCCCCCTTC CAAGCCCAAG AAGAGGGAAG TGGTGGCAGT CACGGACCTC 
CTAGACCCGC CCAACAGGGA GAAACGCCCA GCCAAGATCT GTATGATCCT GCGTGGCCCT 
CCTGGAAGCG GCAAGAGTTA CATTGCAAAG CTTATCAAGG ACAGAGAAAT ACAAAACCGA 
GGTCCTAGTC CTCGCATGCT GTGTCTGGAT GACTACTTTA TGAGTGAGAA GGAGAAGACG 
GAAAAGGACG CTGAAACTGG CAAGATTGTC AAGAAGAGGT TTCAGGAATA CGAACATGAG 
GACAGCATGG AGGCTATTTA CCATGTCAGT TTACTCAAGA CATTCAAGAA GACCATCGAA 
GACGGTTTCT TTGACTTCAT CATCGTGGAT GCCGTCAACG ACAAAATCTC AAAGATTGAA 
CCGTTCTGGA GCTATGCAAA GATGAAAGGA TTTGAGGTGT ATGTAGCTGA GTTACTTGTT 
GAATTAGGCT TGTGCATTCA GCGAGACATC CACAATAGAG GTGAAACAGG AATCAGAAAG 
ATCATAGAAC ACTGGGAGTT TGCCCCACGG AAATACATCA AGCTAGATGT GGTGGCCCTC 
CTGCAAGATG CTGCCATTCC AGAGGTTGAG ATGGAGGAAC TTGATGAAGA CGCCGGACCT 
TCCATGGAGG AACTGATGAA CCAACAGCAG GAAGCCTTGG AAGAACTAGA GGATCAGGAA 
GAGACACTGG CTTTGACGAA GAGTAAGTGG GATTCTGCTA CCGAAGAGAA ACTAGATGCC 
CTTGATGGTA TCAAGAAGTA TAAGAAGAAG GAGAGCTACA CGTCTATGGA GGACTTCCTA 
TCATGTGGAG ATGAGTTTGA CACAGTCTCC AAACCCATAC TCCCAGGACA GAAGAGAGTA 
CGCTGGGCCG ACATCGAGGA ACGCCAAAAC CAAGAACGTA TGCGTGCAGT AGGTTTTGTC 
GTTGGGCAGA CGGCCACTGA CTGGGCCAAG ATCACTGACG ACAATTATGC AGACAAGGCT 
CTCAATCGCA CCAAGTACAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030855691.1
CDS	124..7347
Translation	MFNNWNRPGGPGPGGPGERPPMMPGNGMPNRPPFYGPGSGPGAGGRPMHMSGPPGPGPGSHHMGMRMDSMNPRGPMSGPGHWHGPGMNMMGPGGPGPRRPPPPMTMSMMHGPGPGGPRPRMGMPIPEPVRVLVNQAQAADTAVPQPPQNEDVKLRGLQDQALHWQATAPVRKEEETVQKQAAAQDQWQSYQQQMQSYQQQYQQWYQSQETTLMDVKLKDLQEKKATCEKQLTDWKNQFDNWVEQHKKHPNKDQFDQYQNQWKTWQTQMESMVNSLDMQIKIKETSLQKNSSKNASTTQTPASVGSGVGTVTTQGANVAMPSGGHGMVPSSGAGMGQGSSIGSIPSSQSVATVPSGSGMGAGPRGLGVGPMPTGSGMGPGPSGTNPRVTPALPQGPGQGPRMESGSRSNSGAGLPGPKGQMPGKDSFPFPFPDDFPFPGDMDNFSFPDMNKLDAMFSGGFKSDEFPFSFPDFPMPGGLMGPDFPLPEGLKPEDMTKDMEEFMKQMESELGNVSAKFQPTPRGSNIADQDKQQALNAKASRGPVDIERGPQGMNRDSAGMGRHPMGMDRGPQGMERHSQEFNRGLSGIDRGPRESLQGRERVPPGMDRGQQGMDRCPPGMDRRRPGMDGGPLGMDRGQPGMDRGQPGMDRGTPGMDRGPPGMDRGPTGMDRGQPSLDRGPPGMDRGQQGMDRGQPGMDRGQPGMDRGQPGMDRGPPGMDRGQQRMDRGQQGMDRGYPGMDRGQTGMDRGPPGMDRGQQGMERVPPGTDKRLPGMDRGPPGMDGGPPRLDRGQPGMDKGPPGMNRGPPGMDGGPPGMDRGHPGMDKGPPGMDRGPPGMERGPPGLDRGHPGMDKGPPGMESGHPGIDKGPPGMDRGTAGRNRGQSEMDRGSSRNEGDAPRTNKVQLGIGGILAGLKDSSGMDNDHRMIDRGPPGIDSGPPGADSGPPGVDSGALRTDRSQLALGNVLAGLRAAPPGMDRGPPGIDGGPPGIDSGPPGIEINAPGRTKSQLGLGSILAALKTGPPGMDTGPPGVEMRPSGMDKGPPGIDIAPPVMDMGPPGTDRGPPSNYIKPPGMDNSPPVMGTLPMSLTRGPPGPPGVDVGPPGVDVGPPGVDIDAISMHSHYLGAGKPRPPFDQRQLDIGPPGVDTFPPGIPRYPFGSTQGPAGFGNSSSIQDSSFEEPMPPGMEPISKPLAFPRDLAPPGMDETFPPGMEPEQESQPSTKSKQPQPPEDNISEFEKGQNKPLFPPPSKGLTQHSYSVGQSIPITGMVKDSFGASSFVSTKQTLAPSPLAGYESLESVKAKKEEKKKDESKSEDKKKGKGKKGNMNMPTIIKFGDSKIDKDSDDPVKVEKDETSTDEKAAKEAAKAKEFKKENSPSEEVSKRVTVRSTLPLITDSTTKSMIPSSIGELFKKNSTTPPAFNKDVKTSLRSYRKAAEDDEEEEEAPHSKKMNRKDSSDRDDRGRDDTLRTTLPSPPRSYRSPDRRSRRSPERRRSRSPDKRKRRSRSRSISQDRRKRRRDSPPRKRNSYSPSKRRSPSKSKNSRSPSRDKGNRSPDRRDQRDRRDQRDQRDQRDRRDGRDDRYREKDRRDRYPDKEYEKDSDRRGGKPSTEAFDKLRELIYDTLVRHPDEKPRDSPHSDRRHPDDRSPQRPRRENYYPEAERPRMRYPPPRRDYERDPLRDRLRSLPRDSPRDLPLPAQREPRRDFDRDSSQDRSMERAHSPPVTEITAPIVFDYGHAESTRPAYDIETRDPMGSLLSNAVIVDVVDYSHGQNQGPDARAISGSELLQERESSFPSEFQIHKEIQPAKRDDTSMQQSPAPPHREPSPQPPPQPPMQQSLPQQTQQQELPYREQHQQFSQQYLPDDLTQQFDPYSGQQQIGYYQQPESQMAFQQQQHLQAFQQMGQFPQQAQYNTQQQQQHYTEQFEQQQQQPPPPPPPPSEDLRSSAYRKSPPPPPPDTPAPPKDDSFQQSWAAAPPPPPSDSYTSTRQSGGQDQDRSRERGDDRQRGRGDKQSGGQDQDRSRERGDDRQRGRGDKYQSGPARLVEDYRDSPGQKDMFTNSPQQTPPPPSKPKKREVVAVTDLLDPPNREKRPAKICMILRGPPGSGKSYIAKLIKDREIQNRGPSPRMLCLDDYFMSEKEKTEKDAETGKIVKKRFQEYEHEDSMEAIYHVSLLKTFKKTIEDGFFDFIIVDAVNDKISKIEPFWSYAKMKGFEVYVAELLVELGLCIQRDIHNRGETGIRKIIEHWEFAPRKYIKLDVVALLQDAAIPEVEMEELDEDAGPSMEELMNQQQEALEELEDQEETLALTKSKWDSATEEKLDALDGIKKYKKKESYTSMEDFLSCGDEFDTVSKPILPGQKRVRWADIEERQNQERMRAVGFVVGQTATDWAKITDDNYADKALNRTKYI

Target ORF information:

RefSeq Version	XM_030999831.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus YLP motif-containing protein 1 (LOC581149), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030999831.1

ORF Insert Sequence:

ATGTTCAACA ACTGGAACAG GCCAGGTGGG CCCGGGCCTG GAGGTCCGGG CGAAAGGCCT 
CCGATGATGC CAGGAAATGG AATGCCGAAT CGTCCTCCAT TTTACGGACC AGGATCAGGT 
CCAGGGGCAG GTGGACGACC CATGCATATG AGTGGCCCGC CAGGACCGGG GCCTGGCTCG 
CACCACATGG GAATGAGGAT GGACAGCATG AACCCTCGCG GACCCATGTC GGGCCCGGGG 
CATTGGCATG GACCAGGTAT GAATATGATG GGACCAGGTG GACCAGGACC AAGAAGACCA 
CCTCCACCGA TGACGATGTC GATGATGCAT GGTCCTGGTC CAGGTGGCCC AAGGCCAAGA 
ATGGGTATGC CCATCCCAGA GCCTGTTCGG GTGTTAGTCA ATCAAGCGCA AGCCGCAGAT 
ACTGCAGTCC CACAACCGCC TCAGAATGAA GATGTGAAGT TGAGAGGGCT TCAGGATCAG 
GCCTTACATT GGCAGGCAAC GGCCCCGGTT CGAAAGGAGG AAGAAACTGT ACAAAAACAG 
GCAGCTGCTC AAGACCAGTG GCAGAGCTAC CAGCAACAGA TGCAATCATA TCAGCAACAG 
TATCAACAAT GGTACCAAAG TCAAGAGACT ACCTTGATGG ATGTTAAATT GAAAGACTTG 
CAGGAAAAGA AGGCTACCTG TGAAAAGCAG CTCACAGACT GGAAAAATCA GTTTGATAAT 
TGGGTAGAAC AGCATAAGAA ACATCCAAAC AAGGACCAGT TTGATCAATA TCAAAATCAA 
TGGAAAACAT GGCAAACACA GATGGAGAGC ATGGTGAATT CTTTGGACAT GCAAATCAAA 
ATAAAAGAGA CATCCCTTCA GAAGAACTCC TCTAAAAATG CTTCAACAAC CCAGACACCT 
GCTTCTGTCG GCAGTGGTGT AGGAACAGTT ACCACACAAG GTGCAAATGT AGCAATGCCA 
TCAGGTGGGC ATGGAATGGT TCCATCTAGT GGTGCTGGAA TGGGACAAGG GTCATCAATT 
GGTTCTATAC CAAGCAGTCA GAGTGTAGCC ACAGTTCCAA GTGGTTCTGG GATGGGAGCA 
GGACCCAGAG GACTTGGAGT AGGACCAATG CCAACTGGGA GTGGAATGGG ACCAGGTCCC 
TCAGGAACAA ATCCTAGAGT TACCCCTGCA TTGCCTCAAG GACCTGGGCA AGGGCCAAGA 
ATGGAGAGTG GAAGCAGGTC AAACTCTGGA GCTGGACTTC CAGGCCCAAA AGGACAGATG 
CCTGGCAAAG ACAGCTTTCC TTTCCCATTT CCTGATGATT TTCCATTTCC AGGAGATATG 
GACAATTTCT CCTTCCCTGA TATGAATAAA CTAGATGCAA TGTTTTCAGG TGGTTTTAAA 
TCTGATGAGT TTCCATTTTC CTTCCCTGAT TTTCCGATGC CAGGTGGTTT AATGGGACCC 
GACTTTCCAT TACCTGAAGG ACTGAAACCC GAAGACATGA CCAAAGATAT GGAAGAATTC 
ATGAAACAGA TGGAATCTGA ACTAGGCAAT GTTTCTGCAA AATTTCAACC AACCCCAAGG 
GGAAGCAACA TAGCCGATCA GGATAAACAG CAAGCTTTAA ATGCCAAAGC ATCAAGAGGA 
CCTGTAGACA TAGAGAGGGG TCCACAAGGA ATGAACAGAG ATTCTGCTGG GATGGGAAGA 
CACCCAATGG GAATGGATAG AGGTCCTCAA GGTATGGAGA GACACTCACA AGAATTCAAT 
AGAGGTCTAT CAGGAATAGA CAGAGGCCCA AGAGAAAGTC TTCAAGGGAG GGAAAGGGTC 
CCACCAGGAA TGGATAGAGG ACAACAAGGA ATGGATAGAT GCCCTCCCGG AATGGATAGA 
AGAAGACCAG GCATGGACGG AGGCCCTCTT GGAATGGATA GAGGACAACC AGGAATGGAT 
AGAGGACAAC CAGGAATGGA CAGAGGCACT CCTGGAATGG ATAGAGGTCC CCCTGGGATG 
GATAGGGGAC CAACCGGAAT GGATAGAGGA CAACCAAGCT TGGATAGAGG TCCTCCTGGA 
ATGGATAGAG GACAGCAAGG AATGGATAGA GGTCAACCAG GAATGGATAG AGGACAACCA 
GGAATGGATA GAGGACAACC AGGAATGGAT AGAGGTCCTC CTGGAATGGA TAGAGGACAG 
CAACGAATGG ATAGAGGACA GCAAGGAATG GATAGAGGTT ATCCTGGAAT GGATAGAGGA 
CAAACAGGAA TGGATAGAGG TCCTCCTGGA ATGGATAGAG GACAGCAAGG AATGGAGAGA 
GTTCCTCCTG GAACAGACAA AAGACTACCC GGAATGGATA GAGGCCCTCC TGGAATGGAT 
GGAGGCCCTC CCAGACTGGA TAGAGGACAA CCAGGCATGG ATAAAGGCCC TCCTGGAATG 
AATAGAGGCC CTCCCGGAAT GGATGGAGGC CCTCCCGGAA TGGATAGAGG ACATCCAGGC 
ATGGATAAAG GCCCTCCTGG AATGGATAGA GGCCCTCCCG GAATGGAGAG AGGCCCACCT 
GGACTGGATA GAGGACATCC AGGCATGGAT AAAGGCCCTC CTGGAATGGA GAGTGGACAT 
CCAGGCATAG ATAAAGGCCC TCCTGGAATG GATAGAGGTA CAGCAGGAAG GAATAGAGGC 
CAATCAGAAA TGGATAGAGG CTCATCAAGA AATGAGGGAG ATGCACCAAG AACTAATAAA 
GTTCAACTAG GGATCGGGGG AATTCTTGCA GGACTTAAAG ATTCATCAGG TATGGATAAT 
GATCATAGAA TGATTGACAG AGGCCCACCA GGAATTGACA GTGGCCCTCC AGGAGCAGAC 
AGTGGCCCAC CAGGAGTAGA CAGTGGTGCA TTGAGAACCG ATAGAAGTCA ACTAGCTTTA 
GGGAACGTTC TTGCTGGGTT GAGGGCAGCC CCACCAGGAA TGGATAGAGG CCCACCAGGA 
ATAGACGGTG GCCCACCAGG AATAGACAGT GGCCCACCAG GAATAGAAAT AAACGCCCCA 
GGTCGTACTA AAAGTCAACT AGGTTTAGGG AGCATTCTTG CTGCACTGAA GACAGGTCCA 
CCAGGAATGG ATACGGGTCC ACCTGGTGTG GAGATGAGAC CTAGTGGCAT GGACAAGGGA 
CCCCCGGGAA TAGACATAGC CCCTCCAGTT ATGGATATGG GTCCTCCAGG AACGGACAGA 
GGACCTCCAT CTAATTACAT TAAACCTCCA GGGATGGACA ACTCACCTCC AGTTATGGGG 
ACATTGCCCA TGAGTTTGAC CAGGGGTCCT CCTGGTCCAC CTGGAGTTGA TGTTGGACCT 
CCTGGTGTCG ATGTTGGACC TCCTGGTGTG GATATTGATG CAATTAGTAT GCATAGCCAT 
TATTTGGGAG CTGGAAAGCC TCGCCCTCCA TTTGATCAAA GGCAATTGGA TATTGGTCCA 
CCAGGTGTAG ATACCTTTCC CCCTGGAATT CCACGTTATC CTTTTGGTTC AACACAAGGT 
CCAGCAGGAT TTGGAAACTC ATCTTCAATT CAAGATAGCA GCTTTGAAGA GCCAATGCCT 
CCTGGTATGG AGCCTATTAG TAAACCCCTT GCTTTTCCCA GAGATCTTGC ACCTCCTGGG 
ATGGATGAGA CATTTCCACC AGGGATGGAA CCAGAACAGG AAAGCCAGCC AAGCACAAAA 
AGTAAACAGC CGCAACCACC GGAAGACAAT ATAAGTGAAT TTGAAAAGGG GCAAAACAAG 
CCACTGTTTC CCCCACCATC CAAAGGTTTG ACTCAACATT CCTATTCAGT TGGACAATCC 
ATACCAATTA CAGGTATGGT GAAAGATTCT TTTGGTGCCA GCTCCTTTGT TTCAACAAAA 
CAGACATTAG CTCCAAGCCC ACTTGCTGGG TATGAGTCAT TAGAATCGGT AAAGGCCAAG 
AAAGAAGAAA AGAAAAAAGA TGAATCAAAG TCAGAGGATA AAAAAAAAGG AAAAGGGAAA 
AAGGGAAATA TGAACATGCC TACAATCATC AAATTTGGGG ATTCTAAAAT TGATAAAGAT 
TCTGATGATC CTGTCAAAGT TGAAAAGGAC GAGACATCTA CTGATGAAAA AGCAGCAAAG 
GAGGCTGCAA AAGCTAAGGA GTTTAAAAAA GAAAATTCAC CTTCTGAGGA GGTTTCAAAG 
AGAGTAACTG TGCGTAGCAC CCTACCATTG ATCACAGATT CTACAACCAA GTCTATGATA 
CCTTCATCAA TTGGTGAGCT ATTCAAGAAG AACTCTACAA CACCTCCAGC ATTCAACAAA 
GATGTAAAGA CCTCACTTAG GAGCTACAGG AAAGCAGCAG AAGATGATGA GGAAGAGGAA 
GAAGCGCCTC ACTCTAAAAA GATGAATAGG AAGGATTCTA GCGATAGAGA TGATAGGGGA 
CGGGATGACA CACTCCGCAC TACACTCCCA TCCCCCCCAC GGTCATATCG GTCACCTGAT 
CGAAGGTCTA GGAGATCTCC TGAAAGAAGG AGATCTCGTT CTCCAGACAA AAGAAAGCGT 
AGATCTCGGT CTAGATCGAT CTCACAGGAC CGCAGGAAAC GGAGGCGGGA TTCTCCACCC 
CGTAAGAGAA ACTCATACTC TCCGAGCAAA AGGAGGTCCC CCTCTAAAAG TAAAAATTCA 
CGATCCCCAA GCAGAGACAA GGGCAATAGA TCCCCGGATC GAAGAGATCA GAGAGATCGA 
AGAGATCAGA GAGATCAAAG AGATCAGAGA GATCGAAGAG ATGGGAGAGA TGACAGATAC 
AGAGAAAAGG ACAGGAGGGA CAGGTATCCT GACAAAGAAT ATGAGAAAGA TTCTGATAGG 
AGAGGTGGGA AGCCAAGTAC AGAAGCCTTT GACAAGTTGC GAGAGCTGAT TTATGATACC 
TTGGTGCGCC ATCCTGATGA AAAGCCAAGG GATTCTCCAC ATAGCGATAG GCGACACCCG 
GACGATAGAT CTCCTCAGCG ACCAAGAAGG GAGAACTATT ACCCGGAAGC TGAGAGGCCA 
AGAATGCGAT ACCCTCCACC ACGTAGAGAC TATGAAAGAG ATCCTTTGAG GGATCGGTTA 
AGAAGCCTTC CAAGGGATTC ACCAAGGGAT CTTCCATTGC CAGCTCAAAG GGAGCCTCGT 
AGGGATTTTG ATAGAGATTC CTCTCAAGAC AGATCTATGG AGAGGGCTCA TTCCCCTCCG 
GTGACGGAAA TAACAGCACC TATTGTCTTT GACTATGGGC ATGCAGAGTC CACCAGGCCT 
GCTTATGACA TAGAGACAAG AGATCCCATG GGAAGCCTGC TCAGCAATGC AGTTATTGTA 
GACGTTGTTG ATTATAGTCA TGGTCAGAAT CAGGGACCAG ATGCTAGAGC AATTAGCGGA 
TCAGAACTTC TTCAAGAGCG GGAGTCATCG TTCCCTTCAG AATTTCAAAT ACATAAAGAG 
ATTCAACCCG CAAAGAGAGA TGATACCAGT ATGCAACAAT CACCAGCTCC ACCACATCGT 
GAACCTTCAC CTCAGCCTCC ACCTCAGCCA CCCATGCAGC AATCACTTCC CCAGCAAACT 
CAGCAGCAAG AACTTCCCTA CAGAGAACAG CACCAGCAAT TCTCTCAGCA GTACCTGCCA 
GATGATCTGA CACAACAGTT TGATCCATAC AGTGGCCAGC AACAGATTGG CTATTACCAG 
CAACCAGAAT CACAGATGGC ATTCCAACAA CAACAACACC TGCAAGCATT CCAGCAAATG 
GGCCAGTTCC CCCAGCAGGC TCAGTACAAC ACCCAGCAAC AGCAGCAACA CTATACGGAA 
CAGTTCGAGC AGCAGCAGCA GCAGCCGCCG CCACCACCAC CTCCACCATC TGAAGATTTG 
CGGTCAAGTG CCTACCGCAA GTCCCCTCCC CCGCCGCCAC CTGATACTCC TGCCCCACCG 
AAGGATGATT CCTTTCAGCA ATCTTGGGCA GCTGCACCTC CCCCACCTCC ATCTGACTCT 
TACACCAGTA CCAGACAATC TGGAGGTCAA GACCAAGACA GATCCAGGGA GAGAGGGGAT 
GACAGGCAAC GTGGAAGGGG TGATAAGCAA TCTGGAGGTC AAGACCAAGA CAGATCCAGG 
GAGAGAGGAG ATGACAGGCA ACGTGGAAGG GGGGATAAAT ACCAGAGTGG CCCTGCCAGG 
CTAGTGGAGG ACTACAGGGA CTCCCCTGGA CAGAAGGACA TGTTCACCAA CTCACCCCAG 
CAAACCCCTC CTCCCCCTTC CAAGCCCAAG AAGAGGGAAG TGGTGGCAGT CACGGACCTC 
CTAGACCCGC CCAACAGGGA GAAACGCCCA GCCAAGATCT GTATGATCCT GCGTGGCCCT 
CCTGGAAGCG GCAAGAGTTA CATTGCAAAG CTTATCAAGG ACAGAGAAAT ACAAAACCGA 
GGTCCTAGTC CTCGCATGCT GTGTCTGGAT GACTACTTTA TGAGTGAGAA GGAGAAGACG 
GAAAAGGACG CTGAAACTGG CAAGATTGTC AAGAAGAGGT TTCAGGAATA CGAACATGAG 
GACAGCATGG AGGCTATTTA CCATGTCAGT TTACTCAAGA CATTCAAGAA GACCATCGAA 
GACGGTTTCT TTGACTTCAT CATCGTGGAT GCCGTCAACG ACAAAATCTC AAAGATTGAA 
CCGTTCTGGA GCTATGCAAA GATGAAAGGA TTTGAGGTGT ATGTAGCTGA GTTACTTGTT 
GAATTAGGCT TGTGCATTCA GCGAGACATC CACAATAGAG GTGAAACAGG AATCAGAAAG 
ATCATAGAAC ACTGGGAGTT TGCCCCACGG AAATACATCA AGCTAGATGT GGTGGCCCTC 
CTGCAAGATG CTGCCATTCC AGAGGTTGAG ATGGAGGAAC TTGATGAAGA CGCCGGACCT 
TCCATGGAGG AACTGATGAA CCAACAGCAG GAAGCCTTGG AAGAACTAGA GGATCAGGAA 
GAGACACTGG CTTTGACGAA GAGTAAGTGG GATTCTGCTA CCGAAGAGAA ACTAGATGCC 
CTTGATGGTA TCAAGAAGTA TAAGAAGAAG GAGAGCTACA CGTCTATGGA GGACTTCCTA 
TCATGTGGAG ATGAGTTTGA CACAGTCTCC AAACCCATAC TCCCAGGACA GAAGAGAGTA 
CGCTGGGCCG ACATCGAGGA ACGCCAAAAC CAAGAACGTA TGCGTGCAGT AGGTTTTGTC 
GTTGGGCAGA CGGCCACTGA CTGGGCCAAG ATCACTGACG ACAATTATGC AGACAAGGCT 
CTCAATCGCA CCAAGTACAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC581149 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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