CACNA1B gene cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

mRNA	Protein	Name
XM_031951856.1	XP_031807716.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
XM_031951857.1	XP_031807717.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
XM_031951861.1	XP_031807721.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
XM_031951867.1	XP_031807727.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X18
XM_031951849.1	XP_031807709.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
XM_031951850.1	XP_031807710.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_031951859.1	XP_031807719.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
XM_031951863.1	XP_031807723.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
XM_023494390.1	XP_023350158.1	voltage-dependent N-type calcium channel subunit alpha-1B
XM_031951855.1	XP_031807715.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
XM_031951858.1	XP_031807718.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
XM_031951865.1	XP_031807725.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
XM_003757585.1	XP_003757633.1	voltage-dependent N-type calcium channel subunit alpha-1B
XM_031951848.1	XP_031807708.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_031951852.1	XP_031807712.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_031951853.1	XP_031807713.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
XM_031951854.1	XP_031807714.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
XM_031951860.1	XP_031807720.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
XM_031951864.1	XP_031807724.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
XM_031951866.1	XP_031807726.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa224799	XM_031951848.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224800	XM_031951849.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224801	XM_031951850.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224802	XM_031951852.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224803	XM_031951853.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224804	XM_031951854.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224805	XM_031951855.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224806	XM_031951856.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224807	XM_031951857.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224808	XM_031951858.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224809	XM_031951859.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224810	XM_031951860.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224811	XM_031951861.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224812	XM_031951863.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224813	XM_031951864.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224814	XM_031951865.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224815	XM_031951866.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa224816	XM_031951867.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

CACNA1B ( XM_031951848.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807708.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa224799
Clone ID Related Accession (Same CDS sequence)	XM_031951848.1
Accession Version	XM_031951848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCGAG CTTTATGGGA 
GGAAAAGGGA AAGACATCAG CACAATCAAG TCACTGCGAG TACTCCGAGT CCTGAGACCT 
CTGAAGACCA TCAAACGTTT GCCAAAACTA AAGGCTGTGT TTGACTGTGT GGTAAACTCC 
CTGAAGAATG TCCTCAATAT CCTCATTGTC TACATGCTCT TCATGTTCAT CTTTGCCGTC 
ATTGCTGTGC AGCTCTTCAA AGGAAAATTC TTCTATTGCA CAGATGAATC CAAAGAGCTT 
CAGAGGGATT GCAGGGGTCA GTATTTGGAT TATGAAAAGG ACGAAGTGGA GGCTCAACCC 
AGACAATGGA AGAAATATGA CTTTCACTAT GACAATGTGC TTTGGGCTTT GCTGACTCTT 
TTCACAGTGT CTACAGGAGA AGGGTGGCCA ACGGTACTGA AGCATTCGGT GGATGCAACA 
TATGAAGAAC AGGGTCCAAG TCCTGGCTTC CGGATGGAAC TGTCAATCTT CTACGTGGTC 
TACTTTGTTG TGTTTCCCTT TTTCTTCGTC AATATCTTTG TGGCATTGAT CATCATCACC 
TTCCAGGAGC AGGGAGACAA AGTGATGTCA GAATGCAGCT TGGAGAAGAA TGAGAGGGCT 
TGCATAGACT TTGCCATCAG TGCCAAGCCC CTGACCCGTT ACATGCCTCA GAACAAGCAG 
TCCTTCCAGT ACAAGACATG GACCTTTGTG GTGTCTCCAC CCTTTGAGTA TTTCATCATG 
GCCATGATCG CCCTCAACAC GGCCGTCTTG ATGATGAAGT TCTATGGTGC CCCCTATGAG 
TATGAAATGA TGCTGAAGGG CTTGAATATT GTATTCACTT CAATGTTCTC TATGGAGTGT 
GTGCTGAAGA TCATCGCCTT TGGGGTGCTG AATTATTTCA GAGATGCCTG GAATGTTTTT 
GATTTTGTAA CAGTATTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGGAAACG 
AACAACTTCA TCAATCTCAG CTTCTTGCGG CTCTTTCGGG CAGCCCGACT CATTAAACTG 
CTTCGCCAGG GATATACTAT CCGAATTCTC CTGTGGACCT TTGTTCAGTC CTTCAAGGCT 
CTGCCGTATG TCTGCTTACT CATCGCCATG CTCTTCTTCA TCTACGCCAT CATTGGCATG 
CAGGTCTTTG GAAACATTGC TCTTGATGAT GAAACGAATA TCAACCGGCA CAACAATTTC 
CGGACATTCC TGCAAGCCCT GATGCTGCTG TTCAGGAGTG CTACAGGGGA AGCCTGGCAT 
GAGATCATGT TGTCATGTCT TAGTGACCGT CCCTGTGATC CTGAAACCAA ACTAAAAGAT 
GAATGTGGGA GTGACTTTGC TTACTTTTAC TTCGTCTCCT TCATTTTCCT CTGCTCCTTT 
CTGATGCTGA ATCTCTTTGT GGCAGTGATT ATGGATAATT TTGAGTACCT GACTCGAGAC 
TCTTCCATCC TAGGACCTCA TCACCTGGAC GAATTTATTC GGGTCTGGGC TGAATATGAC 
CCAGCTGCAT GTGGGCGCAT CAGTTACAGT GACATGTTTG AGATGCTGAA ACACATGTCC 
CCACCCCTCG GCCTGGGAAA GAAATGCCCT GCTCGAGTTG CCTATAAGCG CCTGGTGCGA 
ATGAACATGC CCATCTCCAA TGAGGACATG ACTGTGCACT TCACTTCCAC CTTGATGGCC 
CTCATCCGGA CGGCTCTGGA CATCAAGCTT GCACCAGCGG GGATGAAACA GCATCAGTGT 
GATGCAGAAC TGAGGAAAGA GATTGCATCT GTTTGGGCTA ATCTGCCCCA GAAGACACTG 
GACTTACTGG TGCCTCCACA TAAACCTGAT GAGATGACTG TAGGAAAAGT CTACGCAGCT 
CTGATGATTT TTGACTTCTA CAAGCAGAAC AAAAACACCC GAGATCAAGG TCACCAGCCT 
CCGACAGGTC CTGTGTCTCT GTTCCATACC CAAGGCCAAC CAGCTGTGCT CCGAGGTGCT 
AGAGTGTTCT TGAAACAGAA AAGTTCAACC TCCCTAAGCA ATGGTGGAGC CTTACCACAC 
CCAGGGAGTG GTATCAAGGA GTCTGTCTCA TGGGGCACCC AGCGCACCCA GGATATCTGC 
TATGAGACCA GGATGCCCCT GGAACGTGGT CATTCTGCAG AAATTCCTGT GGGACAACCT 
AGCAAACAAG CTGTGGAGAT GCAAGAAATG GCTCAGGATG CCACCAGTGG AGAACCCCAG 
CCAGGGCTGG AAAGTCAGGG CCGAGCGGCC TCCATGCCCC GCCTGGCTGC AGAGATGCAG 
AGGAGCCAAG CGCGATCGCC TGGGAGTTAC CTAGCACCCA TTCCAGACAC AAGCCCCATG 
AAGCGTTCCA TCTCCACCTT GGCCCCACAG CGCCCACATG GCTCACACCT TTATGAATAT 
GGTCTGGAAC GCGTATCCTC AGGCCAGCCT CAGCATCATC ACCATCATCG CTGTCACCGG 
CGCAGGGATA AGAAGCAGAA GTCTTTGGAC AAATGCACTA GTCAGTCAGC AGATGGAGAT 
GGGGCACCAA ACCATAGTGT GGGGATGGCA GCTCCATCAG GGGAAGGTCC AGCCCCATGT 
AAGCATGAGA GGAAGCAAGA AAGAGGGCGA TCCCAGGAGC GGAAGCAGCT CTCTTCCTCC 
TCTTCAGAGA AGCAGCGTTT CTATTCTTGT GACCGATTTG GGAGCCGGGA CCCATCACAG 
CCCAAATCCT CCAATGGAAG TCGTCCTACA TCACCTCCTG GGCAGGAGCC AGGTCCCCAC 
AGACAGGGTA GTGGTTCAGT TAATGGGAGC CCCTTGCTGT CAACATCTGG TGCTAGTACA 
CCGGGCCGTG GTGGGCGGAG GCAGCTCCCC CAGACACCTC TGACCCCCCG TCCCAGCATC 
ACATACAAGA CTGCGAACTC CTCGCCCGTC CACTTCACCG GTTTCCAGAC CAGCCTGCCC 
ACCTTCTCCC CGGGACGGCT CAGTCGAGGC CTTTCTGAGC ACAACACCCT GCTCCAGAGG 
GATTCTCTGA GCCAGCCGCC AACCCCAGTG TCTCGGATTG GCTCGGACCC ATACCTGGGG 
CACCGTGACC ACAGCGATTC CTCGTACCGG GCCCTGCCCG AGGACACACT CACTTTTGAG 
GAAGCCGTCG CCACCAACTC GGGCCGCTCA TCGAGGACTT CCTACGTGTC CTCTCTAACA 
TCCCAGTCCC ACCATCTCCG TCGTGTGCCC AATGGTTACC ACTGCAGTCT GGGGCTCAGC 
TCCGGCCCCG GGACTGGCGG TGGCAGGGCC CGGCAGAGTT ATTATCACCA AACCGACGAA 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807708.1
CDS	1..7155
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAFVKQNRGTVSRSSSASSVNSPKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951848.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951848.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCGAG CTTTATGGGA 
GGAAAAGGGA AAGACATCAG CACAATCAAG TCACTGCGAG TACTCCGAGT CCTGAGACCT 
CTGAAGACCA TCAAACGTTT GCCAAAACTA AAGGCTGTGT TTGACTGTGT GGTAAACTCC 
CTGAAGAATG TCCTCAATAT CCTCATTGTC TACATGCTCT TCATGTTCAT CTTTGCCGTC 
ATTGCTGTGC AGCTCTTCAA AGGAAAATTC TTCTATTGCA CAGATGAATC CAAAGAGCTT 
CAGAGGGATT GCAGGGGTCA GTATTTGGAT TATGAAAAGG ACGAAGTGGA GGCTCAACCC 
AGACAATGGA AGAAATATGA CTTTCACTAT GACAATGTGC TTTGGGCTTT GCTGACTCTT 
TTCACAGTGT CTACAGGAGA AGGGTGGCCA ACGGTACTGA AGCATTCGGT GGATGCAACA 
TATGAAGAAC AGGGTCCAAG TCCTGGCTTC CGGATGGAAC TGTCAATCTT CTACGTGGTC 
TACTTTGTTG TGTTTCCCTT TTTCTTCGTC AATATCTTTG TGGCATTGAT CATCATCACC 
TTCCAGGAGC AGGGAGACAA AGTGATGTCA GAATGCAGCT TGGAGAAGAA TGAGAGGGCT 
TGCATAGACT TTGCCATCAG TGCCAAGCCC CTGACCCGTT ACATGCCTCA GAACAAGCAG 
TCCTTCCAGT ACAAGACATG GACCTTTGTG GTGTCTCCAC CCTTTGAGTA TTTCATCATG 
GCCATGATCG CCCTCAACAC GGCCGTCTTG ATGATGAAGT TCTATGGTGC CCCCTATGAG 
TATGAAATGA TGCTGAAGGG CTTGAATATT GTATTCACTT CAATGTTCTC TATGGAGTGT 
GTGCTGAAGA TCATCGCCTT TGGGGTGCTG AATTATTTCA GAGATGCCTG GAATGTTTTT 
GATTTTGTAA CAGTATTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGGAAACG 
AACAACTTCA TCAATCTCAG CTTCTTGCGG CTCTTTCGGG CAGCCCGACT CATTAAACTG 
CTTCGCCAGG GATATACTAT CCGAATTCTC CTGTGGACCT TTGTTCAGTC CTTCAAGGCT 
CTGCCGTATG TCTGCTTACT CATCGCCATG CTCTTCTTCA TCTACGCCAT CATTGGCATG 
CAGGTCTTTG GAAACATTGC TCTTGATGAT GAAACGAATA TCAACCGGCA CAACAATTTC 
CGGACATTCC TGCAAGCCCT GATGCTGCTG TTCAGGAGTG CTACAGGGGA AGCCTGGCAT 
GAGATCATGT TGTCATGTCT TAGTGACCGT CCCTGTGATC CTGAAACCAA ACTAAAAGAT 
GAATGTGGGA GTGACTTTGC TTACTTTTAC TTCGTCTCCT TCATTTTCCT CTGCTCCTTT 
CTGATGCTGA ATCTCTTTGT GGCAGTGATT ATGGATAATT TTGAGTACCT GACTCGAGAC 
TCTTCCATCC TAGGACCTCA TCACCTGGAC GAATTTATTC GGGTCTGGGC TGAATATGAC 
CCAGCTGCAT GTGGGCGCAT CAGTTACAGT GACATGTTTG AGATGCTGAA ACACATGTCC 
CCACCCCTCG GCCTGGGAAA GAAATGCCCT GCTCGAGTTG CCTATAAGCG CCTGGTGCGA 
ATGAACATGC CCATCTCCAA TGAGGACATG ACTGTGCACT TCACTTCCAC CTTGATGGCC 
CTCATCCGGA CGGCTCTGGA CATCAAGCTT GCACCAGCGG GGATGAAACA GCATCAGTGT 
GATGCAGAAC TGAGGAAAGA GATTGCATCT GTTTGGGCTA ATCTGCCCCA GAAGACACTG 
GACTTACTGG TGCCTCCACA TAAACCTGAT GAGATGACTG TAGGAAAAGT CTACGCAGCT 
CTGATGATTT TTGACTTCTA CAAGCAGAAC AAAAACACCC GAGATCAAGG TCACCAGCCT 
CCGACAGGTC CTGTGTCTCT GTTCCATACC CAAGGCCAAC CAGCTGTGCT CCGAGGTGCT 
AGAGTGTTCT TGAAACAGAA AAGTTCAACC TCCCTAAGCA ATGGTGGAGC CTTACCACAC 
CCAGGGAGTG GTATCAAGGA GTCTGTCTCA TGGGGCACCC AGCGCACCCA GGATATCTGC 
TATGAGACCA GGATGCCCCT GGAACGTGGT CATTCTGCAG AAATTCCTGT GGGACAACCT 
AGCAAACAAG CTGTGGAGAT GCAAGAAATG GCTCAGGATG CCACCAGTGG AGAACCCCAG 
CCAGGGCTGG AAAGTCAGGG CCGAGCGGCC TCCATGCCCC GCCTGGCTGC AGAGATGCAG 
AGGAGCCAAG CGCGATCGCC TGGGAGTTAC CTAGCACCCA TTCCAGACAC AAGCCCCATG 
AAGCGTTCCA TCTCCACCTT GGCCCCACAG CGCCCACATG GCTCACACCT TTATGAATAT 
GGTCTGGAAC GCGTATCCTC AGGCCAGCCT CAGCATCATC ACCATCATCG CTGTCACCGG 
CGCAGGGATA AGAAGCAGAA GTCTTTGGAC AAATGCACTA GTCAGTCAGC AGATGGAGAT 
GGGGCACCAA ACCATAGTGT GGGGATGGCA GCTCCATCAG GGGAAGGTCC AGCCCCATGT 
AAGCATGAGA GGAAGCAAGA AAGAGGGCGA TCCCAGGAGC GGAAGCAGCT CTCTTCCTCC 
TCTTCAGAGA AGCAGCGTTT CTATTCTTGT GACCGATTTG GGAGCCGGGA CCCATCACAG 
CCCAAATCCT CCAATGGAAG TCGTCCTACA TCACCTCCTG GGCAGGAGCC AGGTCCCCAC 
AGACAGGGTA GTGGTTCAGT TAATGGGAGC CCCTTGCTGT CAACATCTGG TGCTAGTACA 
CCGGGCCGTG GTGGGCGGAG GCAGCTCCCC CAGACACCTC TGACCCCCCG TCCCAGCATC 
ACATACAAGA CTGCGAACTC CTCGCCCGTC CACTTCACCG GTTTCCAGAC CAGCCTGCCC 
ACCTTCTCCC CGGGACGGCT CAGTCGAGGC CTTTCTGAGC ACAACACCCT GCTCCAGAGG 
GATTCTCTGA GCCAGCCGCC AACCCCAGTG TCTCGGATTG GCTCGGACCC ATACCTGGGG 
CACCGTGACC ACAGCGATTC CTCGTACCGG GCCCTGCCCG AGGACACACT CACTTTTGAG 
GAAGCCGTCG CCACCAACTC GGGCCGCTCA TCGAGGACTT CCTACGTGTC CTCTCTAACA 
TCCCAGTCCC ACCATCTCCG TCGTGTGCCC AATGGTTACC ACTGCAGTCT GGGGCTCAGC 
TCCGGCCCCG GGACTGGCGG TGGCAGGGCC CGGCAGAGTT ATTATCACCA AACCGACGAA 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951849.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807709.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X2, mRNA.

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CloneID	OSa224800
Clone ID Related Accession (Same CDS sequence)	XM_031951849.1
Accession Version	XM_031951849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCTTT TGTAAAGCAA 
AATCGAGGCA CTGTATCTCG CAGCTCATCC GCCTCCAGCG TAAATTCACC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCGAGCTT TATGGGAGGA 
AAAGGGAAAG ACATCAGCAC AATCAAGTCA CTGCGAGTAC TCCGAGTCCT GAGACCTCTG 
AAGACCATCA AACGTTTGCC AAAACTAAAG GCTGTGTTTG ACTGTGTGGT AAACTCCCTG 
AAGAATGTCC TCAATATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCCGTCATT 
GCTGTGCAGC TCTTCAAAGG AAAATTCTTC TATTGCACAG ATGAATCCAA AGAGCTTCAG 
AGGGATTGCA GGGGTCAGTA TTTGGATTAT GAAAAGGACG AAGTGGAGGC TCAACCCAGA 
CAATGGAAGA AATATGACTT TCACTATGAC AATGTGCTTT GGGCTTTGCT GACTCTTTTC 
ACAGTGTCTA CAGGAGAAGG GTGGCCAACG GTACTGAAGC ATTCGGTGGA TGCAACATAT 
GAAGAACAGG GTCCAAGTCC TGGCTTCCGG ATGGAACTGT CAATCTTCTA CGTGGTCTAC 
TTTGTTGTGT TTCCCTTTTT CTTCGTCAAT ATCTTTGTGG CATTGATCAT CATCACCTTC 
CAGGAGCAGG GAGACAAAGT GATGTCAGAA TGCAGCTTGG AGAAGAATGA GAGGGCTTGC 
ATAGACTTTG CCATCAGTGC CAAGCCCCTG ACCCGTTACA TGCCTCAGAA CAAGCAGTCC 
TTCCAGTACA AGACATGGAC CTTTGTGGTG TCTCCACCCT TTGAGTATTT CATCATGGCC 
ATGATCGCCC TCAACACGGC CGTCTTGATG ATGAAGTTCT ATGGTGCCCC CTATGAGTAT 
GAAATGATGC TGAAGGGCTT GAATATTGTA TTCACTTCAA TGTTCTCTAT GGAGTGTGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAT TATTTCAGAG ATGCCTGGAA TGTTTTTGAT 
TTTGTAACAG TATTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGAAACGAAC 
AACTTCATCA ATCTCAGCTT CTTGCGGCTC TTTCGGGCAG CCCGACTCAT TAAACTGCTT 
CGCCAGGGAT ATACTATCCG AATTCTCCTG TGGACCTTTG TTCAGTCCTT CAAGGCTCTG 
CCGTATGTCT GCTTACTCAT CGCCATGCTC TTCTTCATCT ACGCCATCAT TGGCATGCAG 
GTCTTTGGAA ACATTGCTCT TGATGATGAA ACGAATATCA ACCGGCACAA CAATTTCCGG 
ACATTCCTGC AAGCCCTGAT GCTGCTGTTC AGGAGTGCTA CAGGGGAAGC CTGGCATGAG 
ATCATGTTGT CATGTCTTAG TGACCGTCCC TGTGATCCTG AAACCAAACT AAAAGATGAA 
TGTGGGAGTG ACTTTGCTTA CTTTTACTTC GTCTCCTTCA TTTTCCTCTG CTCCTTTCTG 
ATGCTGAATC TCTTTGTGGC AGTGATTATG GATAATTTTG AGTACCTGAC TCGAGACTCT 
TCCATCCTAG GACCTCATCA CCTGGACGAA TTTATTCGGG TCTGGGCTGA ATATGACCCA 
GCTGCATGTG GGCGCATCAG TTACAGTGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCA 
CCCCTCGGCC TGGGAAAGAA ATGCCCTGCT CGAGTTGCCT ATAAGCGCCT GGTGCGAATG 
AACATGCCCA TCTCCAATGA GGACATGACT GTGCACTTCA CTTCCACCTT GATGGCCCTC 
ATCCGGACGG CTCTGGACAT CAAGCTTGCA CCAGCGGGGA TGAAACAGCA TCAGTGTGAT 
GCAGAACTGA GGAAAGAGAT TGCATCTGTT TGGGCTAATC TGCCCCAGAA GACACTGGAC 
TTACTGGTGC CTCCACATAA ACCTGATGAG ATGACTGTAG GAAAAGTCTA CGCAGCTCTG 
ATGATTTTTG ACTTCTACAA GCAGAACAAA AACACCCGAG ATCAAGGTCA CCAGCCTCCG 
ACAGGTCCTG TGTCTCTGTT CCATACCCAA GGCCAACCAG CTGTGCTCCG AGGTGCTAGA 
GTGTTCTTGA AACAGAAAAG TTCAACCTCC CTAAGCAATG GTGGAGCCTT ACCACACCCA 
GGGAGTGGTA TCAAGGAGTC TGTCTCATGG GGCACCCAGC GCACCCAGGA TATCTGCTAT 
GAGACCAGGA TGCCCCTGGA ACGTGGTCAT TCTGCAGAAA TTCCTGTGGG ACAACCTAGC 
AAACAAGCTG TGGAGATGCA AGAAATGGCT CAGGATGCCA CCAGTGGAGA ACCCCAGCCA 
GGGCTGGAAA GTCAGGGCCG AGCGGCCTCC ATGCCCCGCC TGGCTGCAGA GATGCAGAGG 
AGCCAAGCGC GATCGCCTGG GAGTTACCTA GCACCCATTC CAGACACAAG CCCCATGAAG 
CGTTCCATCT CCACCTTGGC CCCACAGCGC CCACATGGCT CACACCTTTA TGAATATGGT 
CTGGAACGCG TATCCTCAGG CCAGCCTCAG CATCATCACC ATCATCGCTG TCACCGGCGC 
AGGGATAAGA AGCAGAAGTC TTTGGACAAA TGCACTAGTC AGTCAGCAGA TGGAGATGGG 
GCACCAAACC ATAGTGTGGG GATGGCAGCT CCATCAGGGG AAGGTCCAGC CCCATGTAAG 
CATGAGAGGA AGCAAGAAAG AGGGCGATCC CAGGAGCGGA AGCAGCTCTC TTCCTCCTCT 
TCAGAGAAGC AGCGTTTCTA TTCTTGTGAC CGATTTGGGA GCCGGGACCC ATCACAGCCC 
AAATCCTCCA ATGGAAGTCG TCCTACATCA CCTCCTGGGC AGGAGCCAGG TCCCCACAGA 
CAGGGTAGTG GTTCAGTTAA TGGGAGCCCC TTGCTGTCAA CATCTGGTGC TAGTACACCG 
GGCCGTGGTG GGCGGAGGCA GCTCCCCCAG ACACCTCTGA CCCCCCGTCC CAGCATCACA 
TACAAGACTG CGAACTCCTC GCCCGTCCAC TTCACCGGTT TCCAGACCAG CCTGCCCACC 
TTCTCCCCGG GACGGCTCAG TCGAGGCCTT TCTGAGCACA ACACCCTGCT CCAGAGGGAT 
TCTCTGAGCC AGCCGCCAAC CCCAGTGTCT CGGATTGGCT CGGACCCATA CCTGGGGCAC 
CGTGACCACA GCGATTCCTC GTACCGGGCC CTGCCCGAGG ACACACTCAC TTTTGAGGAA 
GCCGTCGCCA CCAACTCGGG CCGCTCATCG AGGACTTCCT ACGTGTCCTC TCTAACATCC 
CAGTCCCACC ATCTCCGTCG TGTGCCCAAT GGTTACCACT GCAGTCTGGG GCTCAGCTCC 
GGCCCCGGGA CTGGCGGTGG CAGGGCCCGG CAGAGTTATT ATCACCAAAC CGACGAAGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807709.1
CDS	1..7152
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAFVKQNRGTVSRSSSASSVNSPKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951849.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951849.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCTTT TGTAAAGCAA 
AATCGAGGCA CTGTATCTCG CAGCTCATCC GCCTCCAGCG TAAATTCACC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCGAGCTT TATGGGAGGA 
AAAGGGAAAG ACATCAGCAC AATCAAGTCA CTGCGAGTAC TCCGAGTCCT GAGACCTCTG 
AAGACCATCA AACGTTTGCC AAAACTAAAG GCTGTGTTTG ACTGTGTGGT AAACTCCCTG 
AAGAATGTCC TCAATATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCCGTCATT 
GCTGTGCAGC TCTTCAAAGG AAAATTCTTC TATTGCACAG ATGAATCCAA AGAGCTTCAG 
AGGGATTGCA GGGGTCAGTA TTTGGATTAT GAAAAGGACG AAGTGGAGGC TCAACCCAGA 
CAATGGAAGA AATATGACTT TCACTATGAC AATGTGCTTT GGGCTTTGCT GACTCTTTTC 
ACAGTGTCTA CAGGAGAAGG GTGGCCAACG GTACTGAAGC ATTCGGTGGA TGCAACATAT 
GAAGAACAGG GTCCAAGTCC TGGCTTCCGG ATGGAACTGT CAATCTTCTA CGTGGTCTAC 
TTTGTTGTGT TTCCCTTTTT CTTCGTCAAT ATCTTTGTGG CATTGATCAT CATCACCTTC 
CAGGAGCAGG GAGACAAAGT GATGTCAGAA TGCAGCTTGG AGAAGAATGA GAGGGCTTGC 
ATAGACTTTG CCATCAGTGC CAAGCCCCTG ACCCGTTACA TGCCTCAGAA CAAGCAGTCC 
TTCCAGTACA AGACATGGAC CTTTGTGGTG TCTCCACCCT TTGAGTATTT CATCATGGCC 
ATGATCGCCC TCAACACGGC CGTCTTGATG ATGAAGTTCT ATGGTGCCCC CTATGAGTAT 
GAAATGATGC TGAAGGGCTT GAATATTGTA TTCACTTCAA TGTTCTCTAT GGAGTGTGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAT TATTTCAGAG ATGCCTGGAA TGTTTTTGAT 
TTTGTAACAG TATTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGAAACGAAC 
AACTTCATCA ATCTCAGCTT CTTGCGGCTC TTTCGGGCAG CCCGACTCAT TAAACTGCTT 
CGCCAGGGAT ATACTATCCG AATTCTCCTG TGGACCTTTG TTCAGTCCTT CAAGGCTCTG 
CCGTATGTCT GCTTACTCAT CGCCATGCTC TTCTTCATCT ACGCCATCAT TGGCATGCAG 
GTCTTTGGAA ACATTGCTCT TGATGATGAA ACGAATATCA ACCGGCACAA CAATTTCCGG 
ACATTCCTGC AAGCCCTGAT GCTGCTGTTC AGGAGTGCTA CAGGGGAAGC CTGGCATGAG 
ATCATGTTGT CATGTCTTAG TGACCGTCCC TGTGATCCTG AAACCAAACT AAAAGATGAA 
TGTGGGAGTG ACTTTGCTTA CTTTTACTTC GTCTCCTTCA TTTTCCTCTG CTCCTTTCTG 
ATGCTGAATC TCTTTGTGGC AGTGATTATG GATAATTTTG AGTACCTGAC TCGAGACTCT 
TCCATCCTAG GACCTCATCA CCTGGACGAA TTTATTCGGG TCTGGGCTGA ATATGACCCA 
GCTGCATGTG GGCGCATCAG TTACAGTGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCA 
CCCCTCGGCC TGGGAAAGAA ATGCCCTGCT CGAGTTGCCT ATAAGCGCCT GGTGCGAATG 
AACATGCCCA TCTCCAATGA GGACATGACT GTGCACTTCA CTTCCACCTT GATGGCCCTC 
ATCCGGACGG CTCTGGACAT CAAGCTTGCA CCAGCGGGGA TGAAACAGCA TCAGTGTGAT 
GCAGAACTGA GGAAAGAGAT TGCATCTGTT TGGGCTAATC TGCCCCAGAA GACACTGGAC 
TTACTGGTGC CTCCACATAA ACCTGATGAG ATGACTGTAG GAAAAGTCTA CGCAGCTCTG 
ATGATTTTTG ACTTCTACAA GCAGAACAAA AACACCCGAG ATCAAGGTCA CCAGCCTCCG 
ACAGGTCCTG TGTCTCTGTT CCATACCCAA GGCCAACCAG CTGTGCTCCG AGGTGCTAGA 
GTGTTCTTGA AACAGAAAAG TTCAACCTCC CTAAGCAATG GTGGAGCCTT ACCACACCCA 
GGGAGTGGTA TCAAGGAGTC TGTCTCATGG GGCACCCAGC GCACCCAGGA TATCTGCTAT 
GAGACCAGGA TGCCCCTGGA ACGTGGTCAT TCTGCAGAAA TTCCTGTGGG ACAACCTAGC 
AAACAAGCTG TGGAGATGCA AGAAATGGCT CAGGATGCCA CCAGTGGAGA ACCCCAGCCA 
GGGCTGGAAA GTCAGGGCCG AGCGGCCTCC ATGCCCCGCC TGGCTGCAGA GATGCAGAGG 
AGCCAAGCGC GATCGCCTGG GAGTTACCTA GCACCCATTC CAGACACAAG CCCCATGAAG 
CGTTCCATCT CCACCTTGGC CCCACAGCGC CCACATGGCT CACACCTTTA TGAATATGGT 
CTGGAACGCG TATCCTCAGG CCAGCCTCAG CATCATCACC ATCATCGCTG TCACCGGCGC 
AGGGATAAGA AGCAGAAGTC TTTGGACAAA TGCACTAGTC AGTCAGCAGA TGGAGATGGG 
GCACCAAACC ATAGTGTGGG GATGGCAGCT CCATCAGGGG AAGGTCCAGC CCCATGTAAG 
CATGAGAGGA AGCAAGAAAG AGGGCGATCC CAGGAGCGGA AGCAGCTCTC TTCCTCCTCT 
TCAGAGAAGC AGCGTTTCTA TTCTTGTGAC CGATTTGGGA GCCGGGACCC ATCACAGCCC 
AAATCCTCCA ATGGAAGTCG TCCTACATCA CCTCCTGGGC AGGAGCCAGG TCCCCACAGA 
CAGGGTAGTG GTTCAGTTAA TGGGAGCCCC TTGCTGTCAA CATCTGGTGC TAGTACACCG 
GGCCGTGGTG GGCGGAGGCA GCTCCCCCAG ACACCTCTGA CCCCCCGTCC CAGCATCACA 
TACAAGACTG CGAACTCCTC GCCCGTCCAC TTCACCGGTT TCCAGACCAG CCTGCCCACC 
TTCTCCCCGG GACGGCTCAG TCGAGGCCTT TCTGAGCACA ACACCCTGCT CCAGAGGGAT 
TCTCTGAGCC AGCCGCCAAC CCCAGTGTCT CGGATTGGCT CGGACCCATA CCTGGGGCAC 
CGTGACCACA GCGATTCCTC GTACCGGGCC CTGCCCGAGG ACACACTCAC TTTTGAGGAA 
GCCGTCGCCA CCAACTCGGG CCGCTCATCG AGGACTTCCT ACGTGTCCTC TCTAACATCC 
CAGTCCCACC ATCTCCGTCG TGTGCCCAAT GGTTACCACT GCAGTCTGGG GCTCAGCTCC 
GGCCCCGGGA CTGGCGGTGG CAGGGCCCGG CAGAGTTATT ATCACCAAAC CGACGAAGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951850.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807710.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.

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CloneID	OSa224801
Clone ID Related Accession (Same CDS sequence)	XM_031951850.1
Accession Version	XM_031951850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCGAG CTTTATGGGA 
GGAAAAGGGA AAGACATCAG CACAATCAAG TCACTGCGAG TACTCCGAGT CCTGAGACCT 
CTGAAGACCA TCAAACGTTT GCCAAAACTA AAGGCTGTGT TTGACTGTGT GGTAAACTCC 
CTGAAGAATG TCCTCAATAT CCTCATTGTC TACATGCTCT TCATGTTCAT CTTTGCCGTC 
ATTGCTGTGC AGCTCTTCAA AGGAAAATTC TTCTATTGCA CAGATGAATC CAAAGAGCTT 
CAGAGGGATT GCAGGGGTCA GTATTTGGAT TATGAAAAGG ACGAAGTGGA GGCTCAACCC 
AGACAATGGA AGAAATATGA CTTTCACTAT GACAATGTGC TTTGGGCTTT GCTGACTCTT 
TTCACAGTGT CTACAGGAGA AGGGTGGCCA ACGGTACTGA AGCATTCGGT GGATGCAACA 
TATGAAGAAC AGGGTCCAAG TCCTGGCTTC CGGATGGAAC TGTCAATCTT CTACGTGGTC 
TACTTTGTTG TGTTTCCCTT TTTCTTCGTC AATATCTTTG TGGCATTGAT CATCATCACC 
TTCCAGGAGC AGGGAGACAA AGTGATGTCA GAATGCAGCT TGGAGAAGAA TGAGAGGGCT 
TGCATAGACT TTGCCATCAG TGCCAAGCCC CTGACCCGTT ACATGCCTCA GAACAAGCAG 
TCCTTCCAGT ACAAGACATG GACCTTTGTG GTGTCTCCAC CCTTTGAGTA TTTCATCATG 
GCCATGATCG CCCTCAACAC GGCCGTCTTG ATGATGAAGT TCTATGGTGC CCCCTATGAG 
TATGAAATGA TGCTGAAGGG CTTGAATATT GTATTCACTT CAATGTTCTC TATGGAGTGT 
GTGCTGAAGA TCATCGCCTT TGGGGTGCTG AATTATTTCA GAGATGCCTG GAATGTTTTT 
GATTTTGTAA CAGTATTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGAACAAC 
TTCATCAATC TCAGCTTCTT GCGGCTCTTT CGGGCAGCCC GACTCATTAA ACTGCTTCGC 
CAGGGATATA CTATCCGAAT TCTCCTGTGG ACCTTTGTTC AGTCCTTCAA GGCTCTGCCG 
TATGTCTGCT TACTCATCGC CATGCTCTTC TTCATCTACG CCATCATTGG CATGCAGGTC 
TTTGGAAACA TTGCTCTTGA TGATGAAACG AATATCAACC GGCACAACAA TTTCCGGACA 
TTCCTGCAAG CCCTGATGCT GCTGTTCAGG AGTGCTACAG GGGAAGCCTG GCATGAGATC 
ATGTTGTCAT GTCTTAGTGA CCGTCCCTGT GATCCTGAAA CCAAACTAAA AGATGAATGT 
GGGAGTGACT TTGCTTACTT TTACTTCGTC TCCTTCATTT TCCTCTGCTC CTTTCTGATG 
CTGAATCTCT TTGTGGCAGT GATTATGGAT AATTTTGAGT ACCTGACTCG AGACTCTTCC 
ATCCTAGGAC CTCATCACCT GGACGAATTT ATTCGGGTCT GGGCTGAATA TGACCCAGCT 
GCATGTGGGC GCATCAGTTA CAGTGACATG TTTGAGATGC TGAAACACAT GTCCCCACCC 
CTCGGCCTGG GAAAGAAATG CCCTGCTCGA GTTGCCTATA AGCGCCTGGT GCGAATGAAC 
ATGCCCATCT CCAATGAGGA CATGACTGTG CACTTCACTT CCACCTTGAT GGCCCTCATC 
CGGACGGCTC TGGACATCAA GCTTGCACCA GCGGGGATGA AACAGCATCA GTGTGATGCA 
GAACTGAGGA AAGAGATTGC ATCTGTTTGG GCTAATCTGC CCCAGAAGAC ACTGGACTTA 
CTGGTGCCTC CACATAAACC TGATGAGATG ACTGTAGGAA AAGTCTACGC AGCTCTGATG 
ATTTTTGACT TCTACAAGCA GAACAAAAAC ACCCGAGATC AAGGTCACCA GCCTCCGACA 
GGTCCTGTGT CTCTGTTCCA TACCCAAGGC CAACCAGCTG TGCTCCGAGG TGCTAGAGTG 
TTCTTGAAAC AGAAAAGTTC AACCTCCCTA AGCAATGGTG GAGCCTTACC ACACCCAGGG 
AGTGGTATCA AGGAGTCTGT CTCATGGGGC ACCCAGCGCA CCCAGGATAT CTGCTATGAG 
ACCAGGATGC CCCTGGAACG TGGTCATTCT GCAGAAATTC CTGTGGGACA ACCTAGCAAA 
CAAGCTGTGG AGATGCAAGA AATGGCTCAG GATGCCACCA GTGGAGAACC CCAGCCAGGG 
CTGGAAAGTC AGGGCCGAGC GGCCTCCATG CCCCGCCTGG CTGCAGAGAT GCAGAGGAGC 
CAAGCGCGAT CGCCTGGGAG TTACCTAGCA CCCATTCCAG ACACAAGCCC CATGAAGCGT 
TCCATCTCCA CCTTGGCCCC ACAGCGCCCA CATGGCTCAC ACCTTTATGA ATATGGTCTG 
GAACGCGTAT CCTCAGGCCA GCCTCAGCAT CATCACCATC ATCGCTGTCA CCGGCGCAGG 
GATAAGAAGC AGAAGTCTTT GGACAAATGC ACTAGTCAGT CAGCAGATGG AGATGGGGCA 
CCAAACCATA GTGTGGGGAT GGCAGCTCCA TCAGGGGAAG GTCCAGCCCC ATGTAAGCAT 
GAGAGGAAGC AAGAAAGAGG GCGATCCCAG GAGCGGAAGC AGCTCTCTTC CTCCTCTTCA 
GAGAAGCAGC GTTTCTATTC TTGTGACCGA TTTGGGAGCC GGGACCCATC ACAGCCCAAA 
TCCTCCAATG GAAGTCGTCC TACATCACCT CCTGGGCAGG AGCCAGGTCC CCACAGACAG 
GGTAGTGGTT CAGTTAATGG GAGCCCCTTG CTGTCAACAT CTGGTGCTAG TACACCGGGC 
CGTGGTGGGC GGAGGCAGCT CCCCCAGACA CCTCTGACCC CCCGTCCCAG CATCACATAC 
AAGACTGCGA ACTCCTCGCC CGTCCACTTC ACCGGTTTCC AGACCAGCCT GCCCACCTTC 
TCCCCGGGAC GGCTCAGTCG AGGCCTTTCT GAGCACAACA CCCTGCTCCA GAGGGATTCT 
CTGAGCCAGC CGCCAACCCC AGTGTCTCGG ATTGGCTCGG ACCCATACCT GGGGCACCGT 
GACCACAGCG ATTCCTCGTA CCGGGCCCTG CCCGAGGACA CACTCACTTT TGAGGAAGCC 
GTCGCCACCA ACTCGGGCCG CTCATCGAGG ACTTCCTACG TGTCCTCTCT AACATCCCAG 
TCCCACCATC TCCGTCGTGT GCCCAATGGT TACCACTGCA GTCTGGGGCT CAGCTCCGGC 
CCCGGGACTG GCGGTGGCAG GGCCCGGCAG AGTTATTATC ACCAAACCGA CGAAGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807710.1
CDS	1..7149
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAFVKQNRGTVSRSSSASSVNSPKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951850.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951850.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCGAG CTTTATGGGA 
GGAAAAGGGA AAGACATCAG CACAATCAAG TCACTGCGAG TACTCCGAGT CCTGAGACCT 
CTGAAGACCA TCAAACGTTT GCCAAAACTA AAGGCTGTGT TTGACTGTGT GGTAAACTCC 
CTGAAGAATG TCCTCAATAT CCTCATTGTC TACATGCTCT TCATGTTCAT CTTTGCCGTC 
ATTGCTGTGC AGCTCTTCAA AGGAAAATTC TTCTATTGCA CAGATGAATC CAAAGAGCTT 
CAGAGGGATT GCAGGGGTCA GTATTTGGAT TATGAAAAGG ACGAAGTGGA GGCTCAACCC 
AGACAATGGA AGAAATATGA CTTTCACTAT GACAATGTGC TTTGGGCTTT GCTGACTCTT 
TTCACAGTGT CTACAGGAGA AGGGTGGCCA ACGGTACTGA AGCATTCGGT GGATGCAACA 
TATGAAGAAC AGGGTCCAAG TCCTGGCTTC CGGATGGAAC TGTCAATCTT CTACGTGGTC 
TACTTTGTTG TGTTTCCCTT TTTCTTCGTC AATATCTTTG TGGCATTGAT CATCATCACC 
TTCCAGGAGC AGGGAGACAA AGTGATGTCA GAATGCAGCT TGGAGAAGAA TGAGAGGGCT 
TGCATAGACT TTGCCATCAG TGCCAAGCCC CTGACCCGTT ACATGCCTCA GAACAAGCAG 
TCCTTCCAGT ACAAGACATG GACCTTTGTG GTGTCTCCAC CCTTTGAGTA TTTCATCATG 
GCCATGATCG CCCTCAACAC GGCCGTCTTG ATGATGAAGT TCTATGGTGC CCCCTATGAG 
TATGAAATGA TGCTGAAGGG CTTGAATATT GTATTCACTT CAATGTTCTC TATGGAGTGT 
GTGCTGAAGA TCATCGCCTT TGGGGTGCTG AATTATTTCA GAGATGCCTG GAATGTTTTT 
GATTTTGTAA CAGTATTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGAACAAC 
TTCATCAATC TCAGCTTCTT GCGGCTCTTT CGGGCAGCCC GACTCATTAA ACTGCTTCGC 
CAGGGATATA CTATCCGAAT TCTCCTGTGG ACCTTTGTTC AGTCCTTCAA GGCTCTGCCG 
TATGTCTGCT TACTCATCGC CATGCTCTTC TTCATCTACG CCATCATTGG CATGCAGGTC 
TTTGGAAACA TTGCTCTTGA TGATGAAACG AATATCAACC GGCACAACAA TTTCCGGACA 
TTCCTGCAAG CCCTGATGCT GCTGTTCAGG AGTGCTACAG GGGAAGCCTG GCATGAGATC 
ATGTTGTCAT GTCTTAGTGA CCGTCCCTGT GATCCTGAAA CCAAACTAAA AGATGAATGT 
GGGAGTGACT TTGCTTACTT TTACTTCGTC TCCTTCATTT TCCTCTGCTC CTTTCTGATG 
CTGAATCTCT TTGTGGCAGT GATTATGGAT AATTTTGAGT ACCTGACTCG AGACTCTTCC 
ATCCTAGGAC CTCATCACCT GGACGAATTT ATTCGGGTCT GGGCTGAATA TGACCCAGCT 
GCATGTGGGC GCATCAGTTA CAGTGACATG TTTGAGATGC TGAAACACAT GTCCCCACCC 
CTCGGCCTGG GAAAGAAATG CCCTGCTCGA GTTGCCTATA AGCGCCTGGT GCGAATGAAC 
ATGCCCATCT CCAATGAGGA CATGACTGTG CACTTCACTT CCACCTTGAT GGCCCTCATC 
CGGACGGCTC TGGACATCAA GCTTGCACCA GCGGGGATGA AACAGCATCA GTGTGATGCA 
GAACTGAGGA AAGAGATTGC ATCTGTTTGG GCTAATCTGC CCCAGAAGAC ACTGGACTTA 
CTGGTGCCTC CACATAAACC TGATGAGATG ACTGTAGGAA AAGTCTACGC AGCTCTGATG 
ATTTTTGACT TCTACAAGCA GAACAAAAAC ACCCGAGATC AAGGTCACCA GCCTCCGACA 
GGTCCTGTGT CTCTGTTCCA TACCCAAGGC CAACCAGCTG TGCTCCGAGG TGCTAGAGTG 
TTCTTGAAAC AGAAAAGTTC AACCTCCCTA AGCAATGGTG GAGCCTTACC ACACCCAGGG 
AGTGGTATCA AGGAGTCTGT CTCATGGGGC ACCCAGCGCA CCCAGGATAT CTGCTATGAG 
ACCAGGATGC CCCTGGAACG TGGTCATTCT GCAGAAATTC CTGTGGGACA ACCTAGCAAA 
CAAGCTGTGG AGATGCAAGA AATGGCTCAG GATGCCACCA GTGGAGAACC CCAGCCAGGG 
CTGGAAAGTC AGGGCCGAGC GGCCTCCATG CCCCGCCTGG CTGCAGAGAT GCAGAGGAGC 
CAAGCGCGAT CGCCTGGGAG TTACCTAGCA CCCATTCCAG ACACAAGCCC CATGAAGCGT 
TCCATCTCCA CCTTGGCCCC ACAGCGCCCA CATGGCTCAC ACCTTTATGA ATATGGTCTG 
GAACGCGTAT CCTCAGGCCA GCCTCAGCAT CATCACCATC ATCGCTGTCA CCGGCGCAGG 
GATAAGAAGC AGAAGTCTTT GGACAAATGC ACTAGTCAGT CAGCAGATGG AGATGGGGCA 
CCAAACCATA GTGTGGGGAT GGCAGCTCCA TCAGGGGAAG GTCCAGCCCC ATGTAAGCAT 
GAGAGGAAGC AAGAAAGAGG GCGATCCCAG GAGCGGAAGC AGCTCTCTTC CTCCTCTTCA 
GAGAAGCAGC GTTTCTATTC TTGTGACCGA TTTGGGAGCC GGGACCCATC ACAGCCCAAA 
TCCTCCAATG GAAGTCGTCC TACATCACCT CCTGGGCAGG AGCCAGGTCC CCACAGACAG 
GGTAGTGGTT CAGTTAATGG GAGCCCCTTG CTGTCAACAT CTGGTGCTAG TACACCGGGC 
CGTGGTGGGC GGAGGCAGCT CCCCCAGACA CCTCTGACCC CCCGTCCCAG CATCACATAC 
AAGACTGCGA ACTCCTCGCC CGTCCACTTC ACCGGTTTCC AGACCAGCCT GCCCACCTTC 
TCCCCGGGAC GGCTCAGTCG AGGCCTTTCT GAGCACAACA CCCTGCTCCA GAGGGATTCT 
CTGAGCCAGC CGCCAACCCC AGTGTCTCGG ATTGGCTCGG ACCCATACCT GGGGCACCGT 
GACCACAGCG ATTCCTCGTA CCGGGCCCTG CCCGAGGACA CACTCACTTT TGAGGAAGCC 
GTCGCCACCA ACTCGGGCCG CTCATCGAGG ACTTCCTACG TGTCCTCTCT AACATCCCAG 
TCCCACCATC TCCGTCGTGT GCCCAATGGT TACCACTGCA GTCTGGGGCT CAGCTCCGGC 
CCCGGGACTG GCGGTGGCAG GGCCCGGCAG AGTTATTATC ACCAAACCGA CGAAGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951852.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807712.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.

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CloneID	OSa224802
Clone ID Related Accession (Same CDS sequence)	XM_031951852.1
Accession Version	XM_031951852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCAGG AAAAGGGAAA 
GACATCAGCA CAATCAAGTC ACTGCGAGTA CTCCGAGTCC TGAGACCTCT GAAGACCATC 
AAACGTTTGC CAAAACTAAA GGCTGTGTTT GACTGTGTGG TAAACTCCCT GAAGAATGTC 
CTCAATATCC TCATTGTCTA CATGCTCTTC ATGTTCATCT TTGCCGTCAT TGCTGTGCAG 
CTCTTCAAAG GAAAATTCTT CTATTGCACA GATGAATCCA AAGAGCTTCA GAGGGATTGC 
AGGGGTCAGT ATTTGGATTA TGAAAAGGAC GAAGTGGAGG CTCAACCCAG ACAATGGAAG 
AAATATGACT TTCACTATGA CAATGTGCTT TGGGCTTTGC TGACTCTTTT CACAGTGTCT 
ACAGGAGAAG GGTGGCCAAC GGTACTGAAG CATTCGGTGG ATGCAACATA TGAAGAACAG 
GGTCCAAGTC CTGGCTTCCG GATGGAACTG TCAATCTTCT ACGTGGTCTA CTTTGTTGTG 
TTTCCCTTTT TCTTCGTCAA TATCTTTGTG GCATTGATCA TCATCACCTT CCAGGAGCAG 
GGAGACAAAG TGATGTCAGA ATGCAGCTTG GAGAAGAATG AGAGGGCTTG CATAGACTTT 
GCCATCAGTG CCAAGCCCCT GACCCGTTAC ATGCCTCAGA ACAAGCAGTC CTTCCAGTAC 
AAGACATGGA CCTTTGTGGT GTCTCCACCC TTTGAGTATT TCATCATGGC CATGATCGCC 
CTCAACACGG CCGTCTTGAT GATGAAGTTC TATGGTGCCC CCTATGAGTA TGAAATGATG 
CTGAAGGGCT TGAATATTGT ATTCACTTCA ATGTTCTCTA TGGAGTGTGT GCTGAAGATC 
ATCGCCTTTG GGGTGCTGAA TTATTTCAGA GATGCCTGGA ATGTTTTTGA TTTTGTAACA 
GTATTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGGAAACGAA CAACTTCATC 
AATCTCAGCT TCTTGCGGCT CTTTCGGGCA GCCCGACTCA TTAAACTGCT TCGCCAGGGA 
TATACTATCC GAATTCTCCT GTGGACCTTT GTTCAGTCCT TCAAGGCTCT GCCGTATGTC 
TGCTTACTCA TCGCCATGCT CTTCTTCATC TACGCCATCA TTGGCATGCA GGTCTTTGGA 
AACATTGCTC TTGATGATGA AACGAATATC AACCGGCACA ACAATTTCCG GACATTCCTG 
CAAGCCCTGA TGCTGCTGTT CAGGAGTGCT ACAGGGGAAG CCTGGCATGA GATCATGTTG 
TCATGTCTTA GTGACCGTCC CTGTGATCCT GAAACCAAAC TAAAAGATGA ATGTGGGAGT 
GACTTTGCTT ACTTTTACTT CGTCTCCTTC ATTTTCCTCT GCTCCTTTCT GATGCTGAAT 
CTCTTTGTGG CAGTGATTAT GGATAATTTT GAGTACCTGA CTCGAGACTC TTCCATCCTA 
GGACCTCATC ACCTGGACGA ATTTATTCGG GTCTGGGCTG AATATGACCC AGCTGCATGT 
GGGCGCATCA GTTACAGTGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC ACCCCTCGGC 
CTGGGAAAGA AATGCCCTGC TCGAGTTGCC TATAAGCGCC TGGTGCGAAT GAACATGCCC 
ATCTCCAATG AGGACATGAC TGTGCACTTC ACTTCCACCT TGATGGCCCT CATCCGGACG 
GCTCTGGACA TCAAGCTTGC ACCAGCGGGG ATGAAACAGC ATCAGTGTGA TGCAGAACTG 
AGGAAAGAGA TTGCATCTGT TTGGGCTAAT CTGCCCCAGA AGACACTGGA CTTACTGGTG 
CCTCCACATA AACCTGATGA GATGACTGTA GGAAAAGTCT ACGCAGCTCT GATGATTTTT 
GACTTCTACA AGCAGAACAA AAACACCCGA GATCAAGGTC ACCAGCCTCC GACAGGTCCT 
GTGTCTCTGT TCCATACCCA AGGCCAACCA GCTGTGCTCC GAGGTGCTAG AGTGTTCTTG 
AAACAGAAAA GTTCAACCTC CCTAAGCAAT GGTGGAGCCT TACCACACCC AGGGAGTGGT 
ATCAAGGAGT CTGTCTCATG GGGCACCCAG CGCACCCAGG ATATCTGCTA TGAGACCAGG 
ATGCCCCTGG AACGTGGTCA TTCTGCAGAA ATTCCTGTGG GACAACCTAG CAAACAAGCT 
GTGGAGATGC AAGAAATGGC TCAGGATGCC ACCAGTGGAG AACCCCAGCC AGGGCTGGAA 
AGTCAGGGCC GAGCGGCCTC CATGCCCCGC CTGGCTGCAG AGATGCAGAG GAGCCAAGCG 
CGATCGCCTG GGAGTTACCT AGCACCCATT CCAGACACAA GCCCCATGAA GCGTTCCATC 
TCCACCTTGG CCCCACAGCG CCCACATGGC TCACACCTTT ATGAATATGG TCTGGAACGC 
GTATCCTCAG GCCAGCCTCA GCATCATCAC CATCATCGCT GTCACCGGCG CAGGGATAAG 
AAGCAGAAGT CTTTGGACAA ATGCACTAGT CAGTCAGCAG ATGGAGATGG GGCACCAAAC 
CATAGTGTGG GGATGGCAGC TCCATCAGGG GAAGGTCCAG CCCCATGTAA GCATGAGAGG 
AAGCAAGAAA GAGGGCGATC CCAGGAGCGG AAGCAGCTCT CTTCCTCCTC TTCAGAGAAG 
CAGCGTTTCT ATTCTTGTGA CCGATTTGGG AGCCGGGACC CATCACAGCC CAAATCCTCC 
AATGGAAGTC GTCCTACATC ACCTCCTGGG CAGGAGCCAG GTCCCCACAG ACAGGGTAGT 
GGTTCAGTTA ATGGGAGCCC CTTGCTGTCA ACATCTGGTG CTAGTACACC GGGCCGTGGT 
GGGCGGAGGC AGCTCCCCCA GACACCTCTG ACCCCCCGTC CCAGCATCAC ATACAAGACT 
GCGAACTCCT CGCCCGTCCA CTTCACCGGT TTCCAGACCA GCCTGCCCAC CTTCTCCCCG 
GGACGGCTCA GTCGAGGCCT TTCTGAGCAC AACACCCTGC TCCAGAGGGA TTCTCTGAGC 
CAGCCGCCAA CCCCAGTGTC TCGGATTGGC TCGGACCCAT ACCTGGGGCA CCGTGACCAC 
AGCGATTCCT CGTACCGGGC CCTGCCCGAG GACACACTCA CTTTTGAGGA AGCCGTCGCC 
ACCAACTCGG GCCGCTCATC GAGGACTTCC TACGTGTCCT CTCTAACATC CCAGTCCCAC 
CATCTCCGTC GTGTGCCCAA TGGTTACCAC TGCAGTCTGG GGCTCAGCTC CGGCCCCGGG 
ACTGGCGGTG GCAGGGCCCG GCAGAGTTAT TATCACCAAA CCGACGAAGA TGACTGGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807712.1
CDS	1..7143
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAFVKQNRGTVSRSSSASSVNSPKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951852.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951852.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCAGG AAAAGGGAAA 
GACATCAGCA CAATCAAGTC ACTGCGAGTA CTCCGAGTCC TGAGACCTCT GAAGACCATC 
AAACGTTTGC CAAAACTAAA GGCTGTGTTT GACTGTGTGG TAAACTCCCT GAAGAATGTC 
CTCAATATCC TCATTGTCTA CATGCTCTTC ATGTTCATCT TTGCCGTCAT TGCTGTGCAG 
CTCTTCAAAG GAAAATTCTT CTATTGCACA GATGAATCCA AAGAGCTTCA GAGGGATTGC 
AGGGGTCAGT ATTTGGATTA TGAAAAGGAC GAAGTGGAGG CTCAACCCAG ACAATGGAAG 
AAATATGACT TTCACTATGA CAATGTGCTT TGGGCTTTGC TGACTCTTTT CACAGTGTCT 
ACAGGAGAAG GGTGGCCAAC GGTACTGAAG CATTCGGTGG ATGCAACATA TGAAGAACAG 
GGTCCAAGTC CTGGCTTCCG GATGGAACTG TCAATCTTCT ACGTGGTCTA CTTTGTTGTG 
TTTCCCTTTT TCTTCGTCAA TATCTTTGTG GCATTGATCA TCATCACCTT CCAGGAGCAG 
GGAGACAAAG TGATGTCAGA ATGCAGCTTG GAGAAGAATG AGAGGGCTTG CATAGACTTT 
GCCATCAGTG CCAAGCCCCT GACCCGTTAC ATGCCTCAGA ACAAGCAGTC CTTCCAGTAC 
AAGACATGGA CCTTTGTGGT GTCTCCACCC TTTGAGTATT TCATCATGGC CATGATCGCC 
CTCAACACGG CCGTCTTGAT GATGAAGTTC TATGGTGCCC CCTATGAGTA TGAAATGATG 
CTGAAGGGCT TGAATATTGT ATTCACTTCA ATGTTCTCTA TGGAGTGTGT GCTGAAGATC 
ATCGCCTTTG GGGTGCTGAA TTATTTCAGA GATGCCTGGA ATGTTTTTGA TTTTGTAACA 
GTATTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGGAAACGAA CAACTTCATC 
AATCTCAGCT TCTTGCGGCT CTTTCGGGCA GCCCGACTCA TTAAACTGCT TCGCCAGGGA 
TATACTATCC GAATTCTCCT GTGGACCTTT GTTCAGTCCT TCAAGGCTCT GCCGTATGTC 
TGCTTACTCA TCGCCATGCT CTTCTTCATC TACGCCATCA TTGGCATGCA GGTCTTTGGA 
AACATTGCTC TTGATGATGA AACGAATATC AACCGGCACA ACAATTTCCG GACATTCCTG 
CAAGCCCTGA TGCTGCTGTT CAGGAGTGCT ACAGGGGAAG CCTGGCATGA GATCATGTTG 
TCATGTCTTA GTGACCGTCC CTGTGATCCT GAAACCAAAC TAAAAGATGA ATGTGGGAGT 
GACTTTGCTT ACTTTTACTT CGTCTCCTTC ATTTTCCTCT GCTCCTTTCT GATGCTGAAT 
CTCTTTGTGG CAGTGATTAT GGATAATTTT GAGTACCTGA CTCGAGACTC TTCCATCCTA 
GGACCTCATC ACCTGGACGA ATTTATTCGG GTCTGGGCTG AATATGACCC AGCTGCATGT 
GGGCGCATCA GTTACAGTGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC ACCCCTCGGC 
CTGGGAAAGA AATGCCCTGC TCGAGTTGCC TATAAGCGCC TGGTGCGAAT GAACATGCCC 
ATCTCCAATG AGGACATGAC TGTGCACTTC ACTTCCACCT TGATGGCCCT CATCCGGACG 
GCTCTGGACA TCAAGCTTGC ACCAGCGGGG ATGAAACAGC ATCAGTGTGA TGCAGAACTG 
AGGAAAGAGA TTGCATCTGT TTGGGCTAAT CTGCCCCAGA AGACACTGGA CTTACTGGTG 
CCTCCACATA AACCTGATGA GATGACTGTA GGAAAAGTCT ACGCAGCTCT GATGATTTTT 
GACTTCTACA AGCAGAACAA AAACACCCGA GATCAAGGTC ACCAGCCTCC GACAGGTCCT 
GTGTCTCTGT TCCATACCCA AGGCCAACCA GCTGTGCTCC GAGGTGCTAG AGTGTTCTTG 
AAACAGAAAA GTTCAACCTC CCTAAGCAAT GGTGGAGCCT TACCACACCC AGGGAGTGGT 
ATCAAGGAGT CTGTCTCATG GGGCACCCAG CGCACCCAGG ATATCTGCTA TGAGACCAGG 
ATGCCCCTGG AACGTGGTCA TTCTGCAGAA ATTCCTGTGG GACAACCTAG CAAACAAGCT 
GTGGAGATGC AAGAAATGGC TCAGGATGCC ACCAGTGGAG AACCCCAGCC AGGGCTGGAA 
AGTCAGGGCC GAGCGGCCTC CATGCCCCGC CTGGCTGCAG AGATGCAGAG GAGCCAAGCG 
CGATCGCCTG GGAGTTACCT AGCACCCATT CCAGACACAA GCCCCATGAA GCGTTCCATC 
TCCACCTTGG CCCCACAGCG CCCACATGGC TCACACCTTT ATGAATATGG TCTGGAACGC 
GTATCCTCAG GCCAGCCTCA GCATCATCAC CATCATCGCT GTCACCGGCG CAGGGATAAG 
AAGCAGAAGT CTTTGGACAA ATGCACTAGT CAGTCAGCAG ATGGAGATGG GGCACCAAAC 
CATAGTGTGG GGATGGCAGC TCCATCAGGG GAAGGTCCAG CCCCATGTAA GCATGAGAGG 
AAGCAAGAAA GAGGGCGATC CCAGGAGCGG AAGCAGCTCT CTTCCTCCTC TTCAGAGAAG 
CAGCGTTTCT ATTCTTGTGA CCGATTTGGG AGCCGGGACC CATCACAGCC CAAATCCTCC 
AATGGAAGTC GTCCTACATC ACCTCCTGGG CAGGAGCCAG GTCCCCACAG ACAGGGTAGT 
GGTTCAGTTA ATGGGAGCCC CTTGCTGTCA ACATCTGGTG CTAGTACACC GGGCCGTGGT 
GGGCGGAGGC AGCTCCCCCA GACACCTCTG ACCCCCCGTC CCAGCATCAC ATACAAGACT 
GCGAACTCCT CGCCCGTCCA CTTCACCGGT TTCCAGACCA GCCTGCCCAC CTTCTCCCCG 
GGACGGCTCA GTCGAGGCCT TTCTGAGCAC AACACCCTGC TCCAGAGGGA TTCTCTGAGC 
CAGCCGCCAA CCCCAGTGTC TCGGATTGGC TCGGACCCAT ACCTGGGGCA CCGTGACCAC 
AGCGATTCCT CGTACCGGGC CCTGCCCGAG GACACACTCA CTTTTGAGGA AGCCGTCGCC 
ACCAACTCGG GCCGCTCATC GAGGACTTCC TACGTGTCCT CTCTAACATC CCAGTCCCAC 
CATCTCCGTC GTGTGCCCAA TGGTTACCAC TGCAGTCTGG GGCTCAGCTC CGGCCCCGGG 
ACTGGCGGTG GCAGGGCCCG GCAGAGTTAT TATCACCAAA CCGACGAAGA TGACTGGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951853.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807713.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.

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CloneID	OSa224803
Clone ID Related Accession (Same CDS sequence)	XM_031951853.1
Accession Version	XM_031951853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCGAG CTTTATGGGA 
GGAAAAGGGA AAGACATCAG CACAATCAAG TCACTGCGAG TACTCCGAGT CCTGAGACCT 
CTGAAGACCA TCAAACGTTT GCCAAAACTA AAGGCTGTGT TTGACTGTGT GGTAAACTCC 
CTGAAGAATG TCCTCAATAT CCTCATTGTC TACATGCTCT TCATGTTCAT CTTTGCCGTC 
ATTGCTGTGC AGCTCTTCAA AGGAAAATTC TTCTATTGCA CAGATGAATC CAAAGAGCTT 
CAGAGGGATT GCAGGGGTCA GTATTTGGAT TATGAAAAGG ACGAAGTGGA GGCTCAACCC 
AGACAATGGA AGAAATATGA CTTTCACTAT GACAATGTGC TTTGGGCTTT GCTGACTCTT 
TTCACAGTGT CTACAGGAGA AGGGTGGCCA ACGGTACTGA AGCATTCGGT GGATGCAACA 
TATGAAGAAC AGGGTCCAAG TCCTGGCTTC CGGATGGAAC TGTCAATCTT CTACGTGGTC 
TACTTTGTTG TGTTTCCCTT TTTCTTCGTC AATATCTTTG TGGCATTGAT CATCATCACC 
TTCCAGGAGC AGGGAGACAA AGTGATGTCA GAATGCAGCT TGGAGAAGAA TGAGAGGGCT 
TGCATAGACT TTGCCATCAG TGCCAAGCCC CTGACCCGTT ACATGCCTCA GAACAAGCAG 
TCCTTCCAGT ACAAGACATG GACCTTTGTG GTGTCTCCAC CCTTTGAGTA TTTCATCATG 
GCCATGATCG CCCTCAACAC GGCCGTCTTG ATGATGAAGT TCTATGGTGC CCCCTATGAG 
TATGAAATGA TGCTGAAGGG CTTGAATATT GTATTCACTT CAATGTTCTC TATGGAGTGT 
GTGCTGAAGA TCATCGCCTT TGGGGTGCTG AATTATTTCA GAGATGCCTG GAATGTTTTT 
GATTTTGTAA CAGTATTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGGAAACG 
AACAACTTCA TCAATCTCAG CTTCTTGCGG CTCTTTCGGG CAGCCCGACT CATTAAACTG 
CTTCGCCAGG GATATACTAT CCGAATTCTC CTGTGGACCT TTGTTCAGTC CTTCAAGGCT 
CTGCCGTATG TCTGCTTACT CATCGCCATG CTCTTCTTCA TCTACGCCAT CATTGGCATG 
CAGGTCTTTG GAAACATTGC TCTTGATGAT GAAACGAATA TCAACCGGCA CAACAATTTC 
CGGACATTCC TGCAAGCCCT GATGCTGCTG TTCAGGAGTG CTACAGGGGA AGCCTGGCAT 
GAGATCATGT TGTCATGTCT TAGTGACCGT CCCTGTGATC CTGAAACCAA ACTAAAAGAT 
GAATGTGGGA GTGACTTTGC TTACTTTTAC TTCGTCTCCT TCATTTTCCT CTGCTCCTTT 
CTGATGCTGA ATCTCTTTGT GGCAGTGATT ATGGATAATT TTGAGTACCT GACTCGAGAC 
TCTTCCATCC TAGGACCTCA TCACCTGGAC GAATTTATTC GGGTCTGGGC TGAATATGAC 
CCAGCTGCAT GTGGGCGCAT CAGTTACAGT GACATGTTTG AGATGCTGAA ACACATGTCC 
CCACCCCTCG GCCTGGGAAA GAAATGCCCT GCTCGAGTTG CCTATAAGCG CCTGGTGCGA 
ATGAACATGC CCATCTCCAA TGAGGACATG ACTGTGCACT TCACTTCCAC CTTGATGGCC 
CTCATCCGGA CGGCTCTGGA CATCAAGCTT GCACCAGCGG GGATGAAACA GCATCAGTGT 
GATGCAGAAC TGAGGAAAGA GATTGCATCT GTTTGGGCTA ATCTGCCCCA GAAGACACTG 
GACTTACTGG TGCCTCCACA TAAACCTGAT GAGATGACTG TAGGAAAAGT CTACGCAGCT 
CTGATGATTT TTGACTTCTA CAAGCAGAAC AAAAACACCC GAGATCAAGG TCACCAGCCT 
CCGACAGGTC CTGTGTCTCT GTTCCATACC CAAGGCCAAC CAGCTGTGCT CCGAGGTGCT 
AGAGTGTTCT TGAAACAGAA AAGTTCAACC TCCCTAAGCA ATGGTGGAGC CTTACCACAC 
CCAGGGAGTG GTATCAAGGA GTCTGTCTCA TGGGGCACCC AGCGCACCCA GGATATCTGC 
TATGAGACCA GGATGCCCCT GGAACGTGGT CATTCTGCAG AAATTCCTGT GGGACAACCT 
AGCAAACAAG CTGTGGAGAT GCAAGAAATG GCTCAGGATG CCACCAGTGG AGAACCCCAG 
CCAGGGCTGG AAAGTCAGGG CCGAGCGGCC TCCATGCCCC GCCTGGCTGC AGAGATGCAG 
CCCATTCCAG ACACAAGCCC CATGAAGCGT TCCATCTCCA CCTTGGCCCC ACAGCGCCCA 
CATGGCTCAC ACCTTTATGA ATATGGTCTG GAACGCGTAT CCTCAGGCCA GCCTCAGCAT 
CATCACCATC ATCGCTGTCA CCGGCGCAGG GATAAGAAGC AGAAGTCTTT GGACAAATGC 
ACTAGTCAGT CAGCAGATGG AGATGGGGCA CCAAACCATA GTGTGGGGAT GGCAGCTCCA 
TCAGGGGAAG GTCCAGCCCC ATGTAAGCAT GAGAGGAAGC AAGAAAGAGG GCGATCCCAG 
GAGCGGAAGC AGCTCTCTTC CTCCTCTTCA GAGAAGCAGC GTTTCTATTC TTGTGACCGA 
TTTGGGAGCC GGGACCCATC ACAGCCCAAA TCCTCCAATG GAAGTCGTCC TACATCACCT 
CCTGGGCAGG AGCCAGGTCC CCACAGACAG GGTAGTGGTT CAGTTAATGG GAGCCCCTTG 
CTGTCAACAT CTGGTGCTAG TACACCGGGC CGTGGTGGGC GGAGGCAGCT CCCCCAGACA 
CCTCTGACCC CCCGTCCCAG CATCACATAC AAGACTGCGA ACTCCTCGCC CGTCCACTTC 
ACCGGTTTCC AGACCAGCCT GCCCACCTTC TCCCCGGGAC GGCTCAGTCG AGGCCTTTCT 
GAGCACAACA CCCTGCTCCA GAGGGATTCT CTGAGCCAGC CGCCAACCCC AGTGTCTCGG 
ATTGGCTCGG ACCCATACCT GGGGCACCGT GACCACAGCG ATTCCTCGTA CCGGGCCCTG 
CCCGAGGACA CACTCACTTT TGAGGAAGCC GTCGCCACCA ACTCGGGCCG CTCATCGAGG 
ACTTCCTACG TGTCCTCTCT AACATCCCAG TCCCACCATC TCCGTCGTGT GCCCAATGGT 
TACCACTGCA GTCTGGGGCT CAGCTCCGGC CCCGGGACTG GCGGTGGCAG GGCCCGGCAG 
AGTTATTATC ACCAAACCGA CGAAGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807713.1
CDS	1..7119
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAFVKQNRGTVSRSSSASSVNSPKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951853.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951853.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCGAG CTTTATGGGA 
GGAAAAGGGA AAGACATCAG CACAATCAAG TCACTGCGAG TACTCCGAGT CCTGAGACCT 
CTGAAGACCA TCAAACGTTT GCCAAAACTA AAGGCTGTGT TTGACTGTGT GGTAAACTCC 
CTGAAGAATG TCCTCAATAT CCTCATTGTC TACATGCTCT TCATGTTCAT CTTTGCCGTC 
ATTGCTGTGC AGCTCTTCAA AGGAAAATTC TTCTATTGCA CAGATGAATC CAAAGAGCTT 
CAGAGGGATT GCAGGGGTCA GTATTTGGAT TATGAAAAGG ACGAAGTGGA GGCTCAACCC 
AGACAATGGA AGAAATATGA CTTTCACTAT GACAATGTGC TTTGGGCTTT GCTGACTCTT 
TTCACAGTGT CTACAGGAGA AGGGTGGCCA ACGGTACTGA AGCATTCGGT GGATGCAACA 
TATGAAGAAC AGGGTCCAAG TCCTGGCTTC CGGATGGAAC TGTCAATCTT CTACGTGGTC 
TACTTTGTTG TGTTTCCCTT TTTCTTCGTC AATATCTTTG TGGCATTGAT CATCATCACC 
TTCCAGGAGC AGGGAGACAA AGTGATGTCA GAATGCAGCT TGGAGAAGAA TGAGAGGGCT 
TGCATAGACT TTGCCATCAG TGCCAAGCCC CTGACCCGTT ACATGCCTCA GAACAAGCAG 
TCCTTCCAGT ACAAGACATG GACCTTTGTG GTGTCTCCAC CCTTTGAGTA TTTCATCATG 
GCCATGATCG CCCTCAACAC GGCCGTCTTG ATGATGAAGT TCTATGGTGC CCCCTATGAG 
TATGAAATGA TGCTGAAGGG CTTGAATATT GTATTCACTT CAATGTTCTC TATGGAGTGT 
GTGCTGAAGA TCATCGCCTT TGGGGTGCTG AATTATTTCA GAGATGCCTG GAATGTTTTT 
GATTTTGTAA CAGTATTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGGAAACG 
AACAACTTCA TCAATCTCAG CTTCTTGCGG CTCTTTCGGG CAGCCCGACT CATTAAACTG 
CTTCGCCAGG GATATACTAT CCGAATTCTC CTGTGGACCT TTGTTCAGTC CTTCAAGGCT 
CTGCCGTATG TCTGCTTACT CATCGCCATG CTCTTCTTCA TCTACGCCAT CATTGGCATG 
CAGGTCTTTG GAAACATTGC TCTTGATGAT GAAACGAATA TCAACCGGCA CAACAATTTC 
CGGACATTCC TGCAAGCCCT GATGCTGCTG TTCAGGAGTG CTACAGGGGA AGCCTGGCAT 
GAGATCATGT TGTCATGTCT TAGTGACCGT CCCTGTGATC CTGAAACCAA ACTAAAAGAT 
GAATGTGGGA GTGACTTTGC TTACTTTTAC TTCGTCTCCT TCATTTTCCT CTGCTCCTTT 
CTGATGCTGA ATCTCTTTGT GGCAGTGATT ATGGATAATT TTGAGTACCT GACTCGAGAC 
TCTTCCATCC TAGGACCTCA TCACCTGGAC GAATTTATTC GGGTCTGGGC TGAATATGAC 
CCAGCTGCAT GTGGGCGCAT CAGTTACAGT GACATGTTTG AGATGCTGAA ACACATGTCC 
CCACCCCTCG GCCTGGGAAA GAAATGCCCT GCTCGAGTTG CCTATAAGCG CCTGGTGCGA 
ATGAACATGC CCATCTCCAA TGAGGACATG ACTGTGCACT TCACTTCCAC CTTGATGGCC 
CTCATCCGGA CGGCTCTGGA CATCAAGCTT GCACCAGCGG GGATGAAACA GCATCAGTGT 
GATGCAGAAC TGAGGAAAGA GATTGCATCT GTTTGGGCTA ATCTGCCCCA GAAGACACTG 
GACTTACTGG TGCCTCCACA TAAACCTGAT GAGATGACTG TAGGAAAAGT CTACGCAGCT 
CTGATGATTT TTGACTTCTA CAAGCAGAAC AAAAACACCC GAGATCAAGG TCACCAGCCT 
CCGACAGGTC CTGTGTCTCT GTTCCATACC CAAGGCCAAC CAGCTGTGCT CCGAGGTGCT 
AGAGTGTTCT TGAAACAGAA AAGTTCAACC TCCCTAAGCA ATGGTGGAGC CTTACCACAC 
CCAGGGAGTG GTATCAAGGA GTCTGTCTCA TGGGGCACCC AGCGCACCCA GGATATCTGC 
TATGAGACCA GGATGCCCCT GGAACGTGGT CATTCTGCAG AAATTCCTGT GGGACAACCT 
AGCAAACAAG CTGTGGAGAT GCAAGAAATG GCTCAGGATG CCACCAGTGG AGAACCCCAG 
CCAGGGCTGG AAAGTCAGGG CCGAGCGGCC TCCATGCCCC GCCTGGCTGC AGAGATGCAG 
CCCATTCCAG ACACAAGCCC CATGAAGCGT TCCATCTCCA CCTTGGCCCC ACAGCGCCCA 
CATGGCTCAC ACCTTTATGA ATATGGTCTG GAACGCGTAT CCTCAGGCCA GCCTCAGCAT 
CATCACCATC ATCGCTGTCA CCGGCGCAGG GATAAGAAGC AGAAGTCTTT GGACAAATGC 
ACTAGTCAGT CAGCAGATGG AGATGGGGCA CCAAACCATA GTGTGGGGAT GGCAGCTCCA 
TCAGGGGAAG GTCCAGCCCC ATGTAAGCAT GAGAGGAAGC AAGAAAGAGG GCGATCCCAG 
GAGCGGAAGC AGCTCTCTTC CTCCTCTTCA GAGAAGCAGC GTTTCTATTC TTGTGACCGA 
TTTGGGAGCC GGGACCCATC ACAGCCCAAA TCCTCCAATG GAAGTCGTCC TACATCACCT 
CCTGGGCAGG AGCCAGGTCC CCACAGACAG GGTAGTGGTT CAGTTAATGG GAGCCCCTTG 
CTGTCAACAT CTGGTGCTAG TACACCGGGC CGTGGTGGGC GGAGGCAGCT CCCCCAGACA 
CCTCTGACCC CCCGTCCCAG CATCACATAC AAGACTGCGA ACTCCTCGCC CGTCCACTTC 
ACCGGTTTCC AGACCAGCCT GCCCACCTTC TCCCCGGGAC GGCTCAGTCG AGGCCTTTCT 
GAGCACAACA CCCTGCTCCA GAGGGATTCT CTGAGCCAGC CGCCAACCCC AGTGTCTCGG 
ATTGGCTCGG ACCCATACCT GGGGCACCGT GACCACAGCG ATTCCTCGTA CCGGGCCCTG 
CCCGAGGACA CACTCACTTT TGAGGAAGCC GTCGCCACCA ACTCGGGCCG CTCATCGAGG 
ACTTCCTACG TGTCCTCTCT AACATCCCAG TCCCACCATC TCCGTCGTGT GCCCAATGGT 
TACCACTGCA GTCTGGGGCT CAGCTCCGGC CCCGGGACTG GCGGTGGCAG GGCCCGGCAG 
AGTTATTATC ACCAAACCGA CGAAGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951854.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807714.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.

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CloneID	OSa224804
Clone ID Related Accession (Same CDS sequence)	XM_031951854.1
Accession Version	XM_031951854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCGAGCTT TATGGGAGGA 
AAAGGGAAAG ACATCAGCAC AATCAAGTCA CTGCGAGTAC TCCGAGTCCT GAGACCTCTG 
AAGACCATCA AACGTTTGCC AAAACTAAAG GCTGTGTTTG ACTGTGTGGT AAACTCCCTG 
AAGAATGTCC TCAATATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCCGTCATT 
GCTGTGCAGC TCTTCAAAGG AAAATTCTTC TATTGCACAG ATGAATCCAA AGAGCTTCAG 
AGGGATTGCA GGGGTCAGTA TTTGGATTAT GAAAAGGACG AAGTGGAGGC TCAACCCAGA 
CAATGGAAGA AATATGACTT TCACTATGAC AATGTGCTTT GGGCTTTGCT GACTCTTTTC 
ACAGTGTCTA CAGGAGAAGG GTGGCCAACG GTACTGAAGC ATTCGGTGGA TGCAACATAT 
GAAGAACAGG GTCCAAGTCC TGGCTTCCGG ATGGAACTGT CAATCTTCTA CGTGGTCTAC 
TTTGTTGTGT TTCCCTTTTT CTTCGTCAAT ATCTTTGTGG CATTGATCAT CATCACCTTC 
CAGGAGCAGG GAGACAAAGT GATGTCAGAA TGCAGCTTGG AGAAGAATGA GAGGGCTTGC 
ATAGACTTTG CCATCAGTGC CAAGCCCCTG ACCCGTTACA TGCCTCAGAA CAAGCAGTCC 
TTCCAGTACA AGACATGGAC CTTTGTGGTG TCTCCACCCT TTGAGTATTT CATCATGGCC 
ATGATCGCCC TCAACACGGC CGTCTTGATG ATGAAGTTCT ATGGTGCCCC CTATGAGTAT 
GAAATGATGC TGAAGGGCTT GAATATTGTA TTCACTTCAA TGTTCTCTAT GGAGTGTGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAT TATTTCAGAG ATGCCTGGAA TGTTTTTGAT 
TTTGTAACAG TATTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGAAACGAAC 
AACTTCATCA ATCTCAGCTT CTTGCGGCTC TTTCGGGCAG CCCGACTCAT TAAACTGCTT 
CGCCAGGGAT ATACTATCCG AATTCTCCTG TGGACCTTTG TTCAGTCCTT CAAGGCTCTG 
CCGTATGTCT GCTTACTCAT CGCCATGCTC TTCTTCATCT ACGCCATCAT TGGCATGCAG 
GTCTTTGGAA ACATTGCTCT TGATGATGAA ACGAATATCA ACCGGCACAA CAATTTCCGG 
ACATTCCTGC AAGCCCTGAT GCTGCTGTTC AGGAGTGCTA CAGGGGAAGC CTGGCATGAG 
ATCATGTTGT CATGTCTTAG TGACCGTCCC TGTGATCCTG AAACCAAACT AAAAGATGAA 
TGTGGGAGTG ACTTTGCTTA CTTTTACTTC GTCTCCTTCA TTTTCCTCTG CTCCTTTCTG 
ATGCTGAATC TCTTTGTGGC AGTGATTATG GATAATTTTG AGTACCTGAC TCGAGACTCT 
TCCATCCTAG GACCTCATCA CCTGGACGAA TTTATTCGGG TCTGGGCTGA ATATGACCCA 
GCTGCATGTG GGCGCATCAG TTACAGTGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCA 
CCCCTCGGCC TGGGAAAGAA ATGCCCTGCT CGAGTTGCCT ATAAGCGCCT GGTGCGAATG 
AACATGCCCA TCTCCAATGA GGACATGACT GTGCACTTCA CTTCCACCTT GATGGCCCTC 
ATCCGGACGG CTCTGGACAT CAAGCTTGCA CCAGCGGGGA TGAAACAGCA TCAGTGTGAT 
GCAGAACTGA GGAAAGAGAT TGCATCTGTT TGGGCTAATC TGCCCCAGAA GACACTGGAC 
TTACTGGTGC CTCCACATAA ACCTGATGAG ATGACTGTAG GAAAAGTCTA CGCAGCTCTG 
ATGATTTTTG ACTTCTACAA GCAGAACAAA AACACCCGAG ATCAAGGTCA CCAGCCTCCG 
ACAGGTCCTG TGTCTCTGTT CCATACCCAA GGCCAACCAG CTGTGCTCCG AGGTGCTAGA 
GTGTTCTTGA AACAGAAAAG TTCAACCTCC CTAAGCAATG GTGGAGCCTT ACCACACCCA 
GGGAGTGGTA TCAAGGAGTC TGTCTCATGG GGCACCCAGC GCACCCAGGA TATCTGCTAT 
GAGACCAGGA TGCCCCTGGA ACGTGGTCAT TCTGCAGAAA TTCCTGTGGG ACAACCTAGC 
AAACAAGCTG TGGAGATGCA AGAAATGGCT CAGGATGCCA CCAGTGGAGA ACCCCAGCCA 
GGGCTGGAAA GTCAGGGCCG AGCGGCCTCC ATGCCCCGCC TGGCTGCAGA GATGCAGAGG 
AGCCAAGCGC GATCGCCTGG GAGTTACCTA GCACCCATTC CAGACACAAG CCCCATGAAG 
CGTTCCATCT CCACCTTGGC CCCACAGCGC CCACATGGCT CACACCTTTA TGAATATGGT 
CTGGAACGCG TATCCTCAGG CCAGCCTCAG CATCATCACC ATCATCGCTG TCACCGGCGC 
AGGGATAAGA AGCAGAAGTC TTTGGACAAA TGCACTAGTC AGTCAGCAGA TGGAGATGGG 
GCACCAAACC ATAGTGTGGG GATGGCAGCT CCATCAGGGG AAGGTCCAGC CCCATGTAAG 
CATGAGAGGA AGCAAGAAAG AGGGCGATCC CAGGAGCGGA AGCAGCTCTC TTCCTCCTCT 
TCAGAGAAGC AGCGTTTCTA TTCTTGTGAC CGATTTGGGA GCCGGGACCC ATCACAGCCC 
AAATCCTCCA ATGGAAGTCG TCCTACATCA CCTCCTGGGC AGGAGCCAGG TCCCCACAGA 
CAGGGTAGTG GTTCAGTTAA TGGGAGCCCC TTGCTGTCAA CATCTGGTGC TAGTACACCG 
GGCCGTGGTG GGCGGAGGCA GCTCCCCCAG ACACCTCTGA CCCCCCGTCC CAGCATCACA 
TACAAGACTG CGAACTCCTC GCCCGTCCAC TTCACCGGTT TCCAGACCAG CCTGCCCACC 
TTCTCCCCGG GACGGCTCAG TCGAGGCCTT TCTGAGCACA ACACCCTGCT CCAGAGGGAT 
TCTCTGAGCC AGCCGCCAAC CCCAGTGTCT CGGATTGGCT CGGACCCATA CCTGGGGCAC 
CGTGACCACA GCGATTCCTC GTACCGGGCC CTGCCCGAGG ACACACTCAC TTTTGAGGAA 
GCCGTCGCCA CCAACTCGGG CCGCTCATCG AGGACTTCCT ACGTGTCCTC TCTAACATCC 
CAGTCCCACC ATCTCCGTCG TGTGCCCAAT GGTTACCACT GCAGTCTGGG GCTCAGCTCC 
GGCCCCGGGA CTGGCGGTGG CAGGGCCCGG CAGAGTTATT ATCACCAAAC CGACGAAGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807714.1
CDS	1..7092
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951854.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951854.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCGAGCTT TATGGGAGGA 
AAAGGGAAAG ACATCAGCAC AATCAAGTCA CTGCGAGTAC TCCGAGTCCT GAGACCTCTG 
AAGACCATCA AACGTTTGCC AAAACTAAAG GCTGTGTTTG ACTGTGTGGT AAACTCCCTG 
AAGAATGTCC TCAATATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCCGTCATT 
GCTGTGCAGC TCTTCAAAGG AAAATTCTTC TATTGCACAG ATGAATCCAA AGAGCTTCAG 
AGGGATTGCA GGGGTCAGTA TTTGGATTAT GAAAAGGACG AAGTGGAGGC TCAACCCAGA 
CAATGGAAGA AATATGACTT TCACTATGAC AATGTGCTTT GGGCTTTGCT GACTCTTTTC 
ACAGTGTCTA CAGGAGAAGG GTGGCCAACG GTACTGAAGC ATTCGGTGGA TGCAACATAT 
GAAGAACAGG GTCCAAGTCC TGGCTTCCGG ATGGAACTGT CAATCTTCTA CGTGGTCTAC 
TTTGTTGTGT TTCCCTTTTT CTTCGTCAAT ATCTTTGTGG CATTGATCAT CATCACCTTC 
CAGGAGCAGG GAGACAAAGT GATGTCAGAA TGCAGCTTGG AGAAGAATGA GAGGGCTTGC 
ATAGACTTTG CCATCAGTGC CAAGCCCCTG ACCCGTTACA TGCCTCAGAA CAAGCAGTCC 
TTCCAGTACA AGACATGGAC CTTTGTGGTG TCTCCACCCT TTGAGTATTT CATCATGGCC 
ATGATCGCCC TCAACACGGC CGTCTTGATG ATGAAGTTCT ATGGTGCCCC CTATGAGTAT 
GAAATGATGC TGAAGGGCTT GAATATTGTA TTCACTTCAA TGTTCTCTAT GGAGTGTGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAT TATTTCAGAG ATGCCTGGAA TGTTTTTGAT 
TTTGTAACAG TATTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGAAACGAAC 
AACTTCATCA ATCTCAGCTT CTTGCGGCTC TTTCGGGCAG CCCGACTCAT TAAACTGCTT 
CGCCAGGGAT ATACTATCCG AATTCTCCTG TGGACCTTTG TTCAGTCCTT CAAGGCTCTG 
CCGTATGTCT GCTTACTCAT CGCCATGCTC TTCTTCATCT ACGCCATCAT TGGCATGCAG 
GTCTTTGGAA ACATTGCTCT TGATGATGAA ACGAATATCA ACCGGCACAA CAATTTCCGG 
ACATTCCTGC AAGCCCTGAT GCTGCTGTTC AGGAGTGCTA CAGGGGAAGC CTGGCATGAG 
ATCATGTTGT CATGTCTTAG TGACCGTCCC TGTGATCCTG AAACCAAACT AAAAGATGAA 
TGTGGGAGTG ACTTTGCTTA CTTTTACTTC GTCTCCTTCA TTTTCCTCTG CTCCTTTCTG 
ATGCTGAATC TCTTTGTGGC AGTGATTATG GATAATTTTG AGTACCTGAC TCGAGACTCT 
TCCATCCTAG GACCTCATCA CCTGGACGAA TTTATTCGGG TCTGGGCTGA ATATGACCCA 
GCTGCATGTG GGCGCATCAG TTACAGTGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCA 
CCCCTCGGCC TGGGAAAGAA ATGCCCTGCT CGAGTTGCCT ATAAGCGCCT GGTGCGAATG 
AACATGCCCA TCTCCAATGA GGACATGACT GTGCACTTCA CTTCCACCTT GATGGCCCTC 
ATCCGGACGG CTCTGGACAT CAAGCTTGCA CCAGCGGGGA TGAAACAGCA TCAGTGTGAT 
GCAGAACTGA GGAAAGAGAT TGCATCTGTT TGGGCTAATC TGCCCCAGAA GACACTGGAC 
TTACTGGTGC CTCCACATAA ACCTGATGAG ATGACTGTAG GAAAAGTCTA CGCAGCTCTG 
ATGATTTTTG ACTTCTACAA GCAGAACAAA AACACCCGAG ATCAAGGTCA CCAGCCTCCG 
ACAGGTCCTG TGTCTCTGTT CCATACCCAA GGCCAACCAG CTGTGCTCCG AGGTGCTAGA 
GTGTTCTTGA AACAGAAAAG TTCAACCTCC CTAAGCAATG GTGGAGCCTT ACCACACCCA 
GGGAGTGGTA TCAAGGAGTC TGTCTCATGG GGCACCCAGC GCACCCAGGA TATCTGCTAT 
GAGACCAGGA TGCCCCTGGA ACGTGGTCAT TCTGCAGAAA TTCCTGTGGG ACAACCTAGC 
AAACAAGCTG TGGAGATGCA AGAAATGGCT CAGGATGCCA CCAGTGGAGA ACCCCAGCCA 
GGGCTGGAAA GTCAGGGCCG AGCGGCCTCC ATGCCCCGCC TGGCTGCAGA GATGCAGAGG 
AGCCAAGCGC GATCGCCTGG GAGTTACCTA GCACCCATTC CAGACACAAG CCCCATGAAG 
CGTTCCATCT CCACCTTGGC CCCACAGCGC CCACATGGCT CACACCTTTA TGAATATGGT 
CTGGAACGCG TATCCTCAGG CCAGCCTCAG CATCATCACC ATCATCGCTG TCACCGGCGC 
AGGGATAAGA AGCAGAAGTC TTTGGACAAA TGCACTAGTC AGTCAGCAGA TGGAGATGGG 
GCACCAAACC ATAGTGTGGG GATGGCAGCT CCATCAGGGG AAGGTCCAGC CCCATGTAAG 
CATGAGAGGA AGCAAGAAAG AGGGCGATCC CAGGAGCGGA AGCAGCTCTC TTCCTCCTCT 
TCAGAGAAGC AGCGTTTCTA TTCTTGTGAC CGATTTGGGA GCCGGGACCC ATCACAGCCC 
AAATCCTCCA ATGGAAGTCG TCCTACATCA CCTCCTGGGC AGGAGCCAGG TCCCCACAGA 
CAGGGTAGTG GTTCAGTTAA TGGGAGCCCC TTGCTGTCAA CATCTGGTGC TAGTACACCG 
GGCCGTGGTG GGCGGAGGCA GCTCCCCCAG ACACCTCTGA CCCCCCGTCC CAGCATCACA 
TACAAGACTG CGAACTCCTC GCCCGTCCAC TTCACCGGTT TCCAGACCAG CCTGCCCACC 
TTCTCCCCGG GACGGCTCAG TCGAGGCCTT TCTGAGCACA ACACCCTGCT CCAGAGGGAT 
TCTCTGAGCC AGCCGCCAAC CCCAGTGTCT CGGATTGGCT CGGACCCATA CCTGGGGCAC 
CGTGACCACA GCGATTCCTC GTACCGGGCC CTGCCCGAGG ACACACTCAC TTTTGAGGAA 
GCCGTCGCCA CCAACTCGGG CCGCTCATCG AGGACTTCCT ACGTGTCCTC TCTAACATCC 
CAGTCCCACC ATCTCCGTCG TGTGCCCAAT GGTTACCACT GCAGTCTGGG GCTCAGCTCC 
GGCCCCGGGA CTGGCGGTGG CAGGGCCCGG CAGAGTTATT ATCACCAAAC CGACGAAGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951855.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807715.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.

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CloneID	OSa224805
Clone ID Related Accession (Same CDS sequence)	XM_031951855.1
Accession Version	XM_031951855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CGAGCTTTAT GGGAGGAAAA 
GGGAAAGACA TCAGCACAAT CAAGTCACTG CGAGTACTCC GAGTCCTGAG ACCTCTGAAG 
ACCATCAAAC GTTTGCCAAA ACTAAAGGCT GTGTTTGACT GTGTGGTAAA CTCCCTGAAG 
AATGTCCTCA ATATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC CGTCATTGCT 
GTGCAGCTCT TCAAAGGAAA ATTCTTCTAT TGCACAGATG AATCCAAAGA GCTTCAGAGG 
GATTGCAGGG GTCAGTATTT GGATTATGAA AAGGACGAAG TGGAGGCTCA ACCCAGACAA 
TGGAAGAAAT ATGACTTTCA CTATGACAAT GTGCTTTGGG CTTTGCTGAC TCTTTTCACA 
GTGTCTACAG GAGAAGGGTG GCCAACGGTA CTGAAGCATT CGGTGGATGC AACATATGAA 
GAACAGGGTC CAAGTCCTGG CTTCCGGATG GAACTGTCAA TCTTCTACGT GGTCTACTTT 
GTTGTGTTTC CCTTTTTCTT CGTCAATATC TTTGTGGCAT TGATCATCAT CACCTTCCAG 
GAGCAGGGAG ACAAAGTGAT GTCAGAATGC AGCTTGGAGA AGAATGAGAG GGCTTGCATA 
GACTTTGCCA TCAGTGCCAA GCCCCTGACC CGTTACATGC CTCAGAACAA GCAGTCCTTC 
CAGTACAAGA CATGGACCTT TGTGGTGTCT CCACCCTTTG AGTATTTCAT CATGGCCATG 
ATCGCCCTCA ACACGGCCGT CTTGATGATG AAGTTCTATG GTGCCCCCTA TGAGTATGAA 
ATGATGCTGA AGGGCTTGAA TATTGTATTC ACTTCAATGT TCTCTATGGA GTGTGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAATTAT TTCAGAGATG CCTGGAATGT TTTTGATTTT 
GTAACAGTAT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAC 
TTCATCAATC TCAGCTTCTT GCGGCTCTTT CGGGCAGCCC GACTCATTAA ACTGCTTCGC 
CAGGGATATA CTATCCGAAT TCTCCTGTGG ACCTTTGTTC AGTCCTTCAA GGCTCTGCCG 
TATGTCTGCT TACTCATCGC CATGCTCTTC TTCATCTACG CCATCATTGG CATGCAGGTC 
TTTGGAAACA TTGCTCTTGA TGATGAAACG AATATCAACC GGCACAACAA TTTCCGGACA 
TTCCTGCAAG CCCTGATGCT GCTGTTCAGG AGTGCTACAG GGGAAGCCTG GCATGAGATC 
ATGTTGTCAT GTCTTAGTGA CCGTCCCTGT GATCCTGAAA CCAAACTAAA AGATGAATGT 
GGGAGTGACT TTGCTTACTT TTACTTCGTC TCCTTCATTT TCCTCTGCTC CTTTCTGATG 
CTGAATCTCT TTGTGGCAGT GATTATGGAT AATTTTGAGT ACCTGACTCG AGACTCTTCC 
ATCCTAGGAC CTCATCACCT GGACGAATTT ATTCGGGTCT GGGCTGAATA TGACCCAGCT 
GCATGTGGGC GCATCAGTTA CAGTGACATG TTTGAGATGC TGAAACACAT GTCCCCACCC 
CTCGGCCTGG GAAAGAAATG CCCTGCTCGA GTTGCCTATA AGCGCCTGGT GCGAATGAAC 
ATGCCCATCT CCAATGAGGA CATGACTGTG CACTTCACTT CCACCTTGAT GGCCCTCATC 
CGGACGGCTC TGGACATCAA GCTTGCACCA GCGGGGATGA AACAGCATCA GTGTGATGCA 
GAACTGAGGA AAGAGATTGC ATCTGTTTGG GCTAATCTGC CCCAGAAGAC ACTGGACTTA 
CTGGTGCCTC CACATAAACC TGATGAGATG ACTGTAGGAA AAGTCTACGC AGCTCTGATG 
ATTTTTGACT TCTACAAGCA GAACAAAAAC ACCCGAGATC AAGGTCACCA GCCTCCGACA 
GGTCCTGTGT CTCTGTTCCA TACCCAAGGC CAACCAGCTG TGCTCCGAGG TGCTAGAGTG 
TTCTTGAAAC AGAAAAGTTC AACCTCCCTA AGCAATGGTG GAGCCTTACC ACACCCAGGG 
AGTGGTATCA AGGAGTCTGT CTCATGGGGC ACCCAGCGCA CCCAGGATAT CTGCTATGAG 
ACCAGGATGC CCCTGGAACG TGGTCATTCT GCAGAAATTC CTGTGGGACA ACCTAGCAAA 
CAAGCTGTGG AGATGCAAGA AATGGCTCAG GATGCCACCA GTGGAGAACC CCAGCCAGGG 
CTGGAAAGTC AGGGCCGAGC GGCCTCCATG CCCCGCCTGG CTGCAGAGAT GCAGAGGAGC 
CAAGCGCGAT CGCCTGGGAG TTACCTAGCA CCCATTCCAG ACACAAGCCC CATGAAGCGT 
TCCATCTCCA CCTTGGCCCC ACAGCGCCCA CATGGCTCAC ACCTTTATGA ATATGGTCTG 
GAACGCGTAT CCTCAGGCCA GCCTCAGCAT CATCACCATC ATCGCTGTCA CCGGCGCAGG 
GATAAGAAGC AGAAGTCTTT GGACAAATGC ACTAGTCAGT CAGCAGATGG AGATGGGGCA 
CCAAACCATA GTGTGGGGAT GGCAGCTCCA TCAGGGGAAG GTCCAGCCCC ATGTAAGCAT 
GAGAGGAAGC AAGAAAGAGG GCGATCCCAG GAGCGGAAGC AGCTCTCTTC CTCCTCTTCA 
GAGAAGCAGC GTTTCTATTC TTGTGACCGA TTTGGGAGCC GGGACCCATC ACAGCCCAAA 
TCCTCCAATG GAAGTCGTCC TACATCACCT CCTGGGCAGG AGCCAGGTCC CCACAGACAG 
GGTAGTGGTT CAGTTAATGG GAGCCCCTTG CTGTCAACAT CTGGTGCTAG TACACCGGGC 
CGTGGTGGGC GGAGGCAGCT CCCCCAGACA CCTCTGACCC CCCGTCCCAG CATCACATAC 
AAGACTGCGA ACTCCTCGCC CGTCCACTTC ACCGGTTTCC AGACCAGCCT GCCCACCTTC 
TCCCCGGGAC GGCTCAGTCG AGGCCTTTCT GAGCACAACA CCCTGCTCCA GAGGGATTCT 
CTGAGCCAGC CGCCAACCCC AGTGTCTCGG ATTGGCTCGG ACCCATACCT GGGGCACCGT 
GACCACAGCG ATTCCTCGTA CCGGGCCCTG CCCGAGGACA CACTCACTTT TGAGGAAGCC 
GTCGCCACCA ACTCGGGCCG CTCATCGAGG ACTTCCTACG TGTCCTCTCT AACATCCCAG 
TCCCACCATC TCCGTCGTGT GCCCAATGGT TACCACTGCA GTCTGGGGCT CAGCTCCGGC 
CCCGGGACTG GCGGTGGCAG GGCCCGGCAG AGTTATTATC ACCAAACCGA CGAAGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807715.1
CDS	1..7089
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951855.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951855.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CGAGCTTTAT GGGAGGAAAA 
GGGAAAGACA TCAGCACAAT CAAGTCACTG CGAGTACTCC GAGTCCTGAG ACCTCTGAAG 
ACCATCAAAC GTTTGCCAAA ACTAAAGGCT GTGTTTGACT GTGTGGTAAA CTCCCTGAAG 
AATGTCCTCA ATATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC CGTCATTGCT 
GTGCAGCTCT TCAAAGGAAA ATTCTTCTAT TGCACAGATG AATCCAAAGA GCTTCAGAGG 
GATTGCAGGG GTCAGTATTT GGATTATGAA AAGGACGAAG TGGAGGCTCA ACCCAGACAA 
TGGAAGAAAT ATGACTTTCA CTATGACAAT GTGCTTTGGG CTTTGCTGAC TCTTTTCACA 
GTGTCTACAG GAGAAGGGTG GCCAACGGTA CTGAAGCATT CGGTGGATGC AACATATGAA 
GAACAGGGTC CAAGTCCTGG CTTCCGGATG GAACTGTCAA TCTTCTACGT GGTCTACTTT 
GTTGTGTTTC CCTTTTTCTT CGTCAATATC TTTGTGGCAT TGATCATCAT CACCTTCCAG 
GAGCAGGGAG ACAAAGTGAT GTCAGAATGC AGCTTGGAGA AGAATGAGAG GGCTTGCATA 
GACTTTGCCA TCAGTGCCAA GCCCCTGACC CGTTACATGC CTCAGAACAA GCAGTCCTTC 
CAGTACAAGA CATGGACCTT TGTGGTGTCT CCACCCTTTG AGTATTTCAT CATGGCCATG 
ATCGCCCTCA ACACGGCCGT CTTGATGATG AAGTTCTATG GTGCCCCCTA TGAGTATGAA 
ATGATGCTGA AGGGCTTGAA TATTGTATTC ACTTCAATGT TCTCTATGGA GTGTGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAATTAT TTCAGAGATG CCTGGAATGT TTTTGATTTT 
GTAACAGTAT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAC 
TTCATCAATC TCAGCTTCTT GCGGCTCTTT CGGGCAGCCC GACTCATTAA ACTGCTTCGC 
CAGGGATATA CTATCCGAAT TCTCCTGTGG ACCTTTGTTC AGTCCTTCAA GGCTCTGCCG 
TATGTCTGCT TACTCATCGC CATGCTCTTC TTCATCTACG CCATCATTGG CATGCAGGTC 
TTTGGAAACA TTGCTCTTGA TGATGAAACG AATATCAACC GGCACAACAA TTTCCGGACA 
TTCCTGCAAG CCCTGATGCT GCTGTTCAGG AGTGCTACAG GGGAAGCCTG GCATGAGATC 
ATGTTGTCAT GTCTTAGTGA CCGTCCCTGT GATCCTGAAA CCAAACTAAA AGATGAATGT 
GGGAGTGACT TTGCTTACTT TTACTTCGTC TCCTTCATTT TCCTCTGCTC CTTTCTGATG 
CTGAATCTCT TTGTGGCAGT GATTATGGAT AATTTTGAGT ACCTGACTCG AGACTCTTCC 
ATCCTAGGAC CTCATCACCT GGACGAATTT ATTCGGGTCT GGGCTGAATA TGACCCAGCT 
GCATGTGGGC GCATCAGTTA CAGTGACATG TTTGAGATGC TGAAACACAT GTCCCCACCC 
CTCGGCCTGG GAAAGAAATG CCCTGCTCGA GTTGCCTATA AGCGCCTGGT GCGAATGAAC 
ATGCCCATCT CCAATGAGGA CATGACTGTG CACTTCACTT CCACCTTGAT GGCCCTCATC 
CGGACGGCTC TGGACATCAA GCTTGCACCA GCGGGGATGA AACAGCATCA GTGTGATGCA 
GAACTGAGGA AAGAGATTGC ATCTGTTTGG GCTAATCTGC CCCAGAAGAC ACTGGACTTA 
CTGGTGCCTC CACATAAACC TGATGAGATG ACTGTAGGAA AAGTCTACGC AGCTCTGATG 
ATTTTTGACT TCTACAAGCA GAACAAAAAC ACCCGAGATC AAGGTCACCA GCCTCCGACA 
GGTCCTGTGT CTCTGTTCCA TACCCAAGGC CAACCAGCTG TGCTCCGAGG TGCTAGAGTG 
TTCTTGAAAC AGAAAAGTTC AACCTCCCTA AGCAATGGTG GAGCCTTACC ACACCCAGGG 
AGTGGTATCA AGGAGTCTGT CTCATGGGGC ACCCAGCGCA CCCAGGATAT CTGCTATGAG 
ACCAGGATGC CCCTGGAACG TGGTCATTCT GCAGAAATTC CTGTGGGACA ACCTAGCAAA 
CAAGCTGTGG AGATGCAAGA AATGGCTCAG GATGCCACCA GTGGAGAACC CCAGCCAGGG 
CTGGAAAGTC AGGGCCGAGC GGCCTCCATG CCCCGCCTGG CTGCAGAGAT GCAGAGGAGC 
CAAGCGCGAT CGCCTGGGAG TTACCTAGCA CCCATTCCAG ACACAAGCCC CATGAAGCGT 
TCCATCTCCA CCTTGGCCCC ACAGCGCCCA CATGGCTCAC ACCTTTATGA ATATGGTCTG 
GAACGCGTAT CCTCAGGCCA GCCTCAGCAT CATCACCATC ATCGCTGTCA CCGGCGCAGG 
GATAAGAAGC AGAAGTCTTT GGACAAATGC ACTAGTCAGT CAGCAGATGG AGATGGGGCA 
CCAAACCATA GTGTGGGGAT GGCAGCTCCA TCAGGGGAAG GTCCAGCCCC ATGTAAGCAT 
GAGAGGAAGC AAGAAAGAGG GCGATCCCAG GAGCGGAAGC AGCTCTCTTC CTCCTCTTCA 
GAGAAGCAGC GTTTCTATTC TTGTGACCGA TTTGGGAGCC GGGACCCATC ACAGCCCAAA 
TCCTCCAATG GAAGTCGTCC TACATCACCT CCTGGGCAGG AGCCAGGTCC CCACAGACAG 
GGTAGTGGTT CAGTTAATGG GAGCCCCTTG CTGTCAACAT CTGGTGCTAG TACACCGGGC 
CGTGGTGGGC GGAGGCAGCT CCCCCAGACA CCTCTGACCC CCCGTCCCAG CATCACATAC 
AAGACTGCGA ACTCCTCGCC CGTCCACTTC ACCGGTTTCC AGACCAGCCT GCCCACCTTC 
TCCCCGGGAC GGCTCAGTCG AGGCCTTTCT GAGCACAACA CCCTGCTCCA GAGGGATTCT 
CTGAGCCAGC CGCCAACCCC AGTGTCTCGG ATTGGCTCGG ACCCATACCT GGGGCACCGT 
GACCACAGCG ATTCCTCGTA CCGGGCCCTG CCCGAGGACA CACTCACTTT TGAGGAAGCC 
GTCGCCACCA ACTCGGGCCG CTCATCGAGG ACTTCCTACG TGTCCTCTCT AACATCCCAG 
TCCCACCATC TCCGTCGTGT GCCCAATGGT TACCACTGCA GTCTGGGGCT CAGCTCCGGC 
CCCGGGACTG GCGGTGGCAG GGCCCGGCAG AGTTATTATC ACCAAACCGA CGAAGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951856.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807716.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.

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CloneID	OSa224806
Clone ID Related Accession (Same CDS sequence)	XM_031951856.1
Accession Version	XM_031951856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCGAGCTT TATGGGAGGA 
AAAGGGAAAG ACATCAGCAC AATCAAGTCA CTGCGAGTAC TCCGAGTCCT GAGACCTCTG 
AAGACCATCA AACGTTTGCC AAAACTAAAG GCTGTGTTTG ACTGTGTGGT AAACTCCCTG 
AAGAATGTCC TCAATATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCCGTCATT 
GCTGTGCAGC TCTTCAAAGG AAAATTCTTC TATTGCACAG ATGAATCCAA AGAGCTTCAG 
AGGGATTGCA GGGGTCAGTA TTTGGATTAT GAAAAGGACG AAGTGGAGGC TCAACCCAGA 
CAATGGAAGA AATATGACTT TCACTATGAC AATGTGCTTT GGGCTTTGCT GACTCTTTTC 
ACAGTGTCTA CAGGAGAAGG GTGGCCAACG GTACTGAAGC ATTCGGTGGA TGCAACATAT 
GAAGAACAGG GTCCAAGTCC TGGCTTCCGG ATGGAACTGT CAATCTTCTA CGTGGTCTAC 
TTTGTTGTGT TTCCCTTTTT CTTCGTCAAT ATCTTTGTGG CATTGATCAT CATCACCTTC 
CAGGAGCAGG GAGACAAAGT GATGTCAGAA TGCAGCTTGG AGAAGAATGA GAGGGCTTGC 
ATAGACTTTG CCATCAGTGC CAAGCCCCTG ACCCGTTACA TGCCTCAGAA CAAGCAGTCC 
TTCCAGTACA AGACATGGAC CTTTGTGGTG TCTCCACCCT TTGAGTATTT CATCATGGCC 
ATGATCGCCC TCAACACGGC CGTCTTGATG ATGAAGTTCT ATGGTGCCCC CTATGAGTAT 
GAAATGATGC TGAAGGGCTT GAATATTGTA TTCACTTCAA TGTTCTCTAT GGAGTGTGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAT TATTTCAGAG ATGCCTGGAA TGTTTTTGAT 
TTTGTAACAG TATTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GAACAACTTC 
ATCAATCTCA GCTTCTTGCG GCTCTTTCGG GCAGCCCGAC TCATTAAACT GCTTCGCCAG 
GGATATACTA TCCGAATTCT CCTGTGGACC TTTGTTCAGT CCTTCAAGGC TCTGCCGTAT 
GTCTGCTTAC TCATCGCCAT GCTCTTCTTC ATCTACGCCA TCATTGGCAT GCAGGTCTTT 
GGAAACATTG CTCTTGATGA TGAAACGAAT ATCAACCGGC ACAACAATTT CCGGACATTC 
CTGCAAGCCC TGATGCTGCT GTTCAGGAGT GCTACAGGGG AAGCCTGGCA TGAGATCATG 
TTGTCATGTC TTAGTGACCG TCCCTGTGAT CCTGAAACCA AACTAAAAGA TGAATGTGGG 
AGTGACTTTG CTTACTTTTA CTTCGTCTCC TTCATTTTCC TCTGCTCCTT TCTGATGCTG 
AATCTCTTTG TGGCAGTGAT TATGGATAAT TTTGAGTACC TGACTCGAGA CTCTTCCATC 
CTAGGACCTC ATCACCTGGA CGAATTTATT CGGGTCTGGG CTGAATATGA CCCAGCTGCA 
TGTGGGCGCA TCAGTTACAG TGACATGTTT GAGATGCTGA AACACATGTC CCCACCCCTC 
GGCCTGGGAA AGAAATGCCC TGCTCGAGTT GCCTATAAGC GCCTGGTGCG AATGAACATG 
CCCATCTCCA ATGAGGACAT GACTGTGCAC TTCACTTCCA CCTTGATGGC CCTCATCCGG 
ACGGCTCTGG ACATCAAGCT TGCACCAGCG GGGATGAAAC AGCATCAGTG TGATGCAGAA 
CTGAGGAAAG AGATTGCATC TGTTTGGGCT AATCTGCCCC AGAAGACACT GGACTTACTG 
GTGCCTCCAC ATAAACCTGA TGAGATGACT GTAGGAAAAG TCTACGCAGC TCTGATGATT 
TTTGACTTCT ACAAGCAGAA CAAAAACACC CGAGATCAAG GTCACCAGCC TCCGACAGGT 
CCTGTGTCTC TGTTCCATAC CCAAGGCCAA CCAGCTGTGC TCCGAGGTGC TAGAGTGTTC 
TTGAAACAGA AAAGTTCAAC CTCCCTAAGC AATGGTGGAG CCTTACCACA CCCAGGGAGT 
GGTATCAAGG AGTCTGTCTC ATGGGGCACC CAGCGCACCC AGGATATCTG CTATGAGACC 
AGGATGCCCC TGGAACGTGG TCATTCTGCA GAAATTCCTG TGGGACAACC TAGCAAACAA 
GCTGTGGAGA TGCAAGAAAT GGCTCAGGAT GCCACCAGTG GAGAACCCCA GCCAGGGCTG 
GAAAGTCAGG GCCGAGCGGC CTCCATGCCC CGCCTGGCTG CAGAGATGCA GAGGAGCCAA 
GCGCGATCGC CTGGGAGTTA CCTAGCACCC ATTCCAGACA CAAGCCCCAT GAAGCGTTCC 
ATCTCCACCT TGGCCCCACA GCGCCCACAT GGCTCACACC TTTATGAATA TGGTCTGGAA 
CGCGTATCCT CAGGCCAGCC TCAGCATCAT CACCATCATC GCTGTCACCG GCGCAGGGAT 
AAGAAGCAGA AGTCTTTGGA CAAATGCACT AGTCAGTCAG CAGATGGAGA TGGGGCACCA 
AACCATAGTG TGGGGATGGC AGCTCCATCA GGGGAAGGTC CAGCCCCATG TAAGCATGAG 
AGGAAGCAAG AAAGAGGGCG ATCCCAGGAG CGGAAGCAGC TCTCTTCCTC CTCTTCAGAG 
AAGCAGCGTT TCTATTCTTG TGACCGATTT GGGAGCCGGG ACCCATCACA GCCCAAATCC 
TCCAATGGAA GTCGTCCTAC ATCACCTCCT GGGCAGGAGC CAGGTCCCCA CAGACAGGGT 
AGTGGTTCAG TTAATGGGAG CCCCTTGCTG TCAACATCTG GTGCTAGTAC ACCGGGCCGT 
GGTGGGCGGA GGCAGCTCCC CCAGACACCT CTGACCCCCC GTCCCAGCAT CACATACAAG 
ACTGCGAACT CCTCGCCCGT CCACTTCACC GGTTTCCAGA CCAGCCTGCC CACCTTCTCC 
CCGGGACGGC TCAGTCGAGG CCTTTCTGAG CACAACACCC TGCTCCAGAG GGATTCTCTG 
AGCCAGCCGC CAACCCCAGT GTCTCGGATT GGCTCGGACC CATACCTGGG GCACCGTGAC 
CACAGCGATT CCTCGTACCG GGCCCTGCCC GAGGACACAC TCACTTTTGA GGAAGCCGTC 
GCCACCAACT CGGGCCGCTC ATCGAGGACT TCCTACGTGT CCTCTCTAAC ATCCCAGTCC 
CACCATCTCC GTCGTGTGCC CAATGGTTAC CACTGCAGTC TGGGGCTCAG CTCCGGCCCC 
GGGACTGGCG GTGGCAGGGC CCGGCAGAGT TATTATCACC AAACCGACGA AGATGACTGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807716.1
CDS	1..7086
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951856.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951856.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCGAGCTT TATGGGAGGA 
AAAGGGAAAG ACATCAGCAC AATCAAGTCA CTGCGAGTAC TCCGAGTCCT GAGACCTCTG 
AAGACCATCA AACGTTTGCC AAAACTAAAG GCTGTGTTTG ACTGTGTGGT AAACTCCCTG 
AAGAATGTCC TCAATATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCCGTCATT 
GCTGTGCAGC TCTTCAAAGG AAAATTCTTC TATTGCACAG ATGAATCCAA AGAGCTTCAG 
AGGGATTGCA GGGGTCAGTA TTTGGATTAT GAAAAGGACG AAGTGGAGGC TCAACCCAGA 
CAATGGAAGA AATATGACTT TCACTATGAC AATGTGCTTT GGGCTTTGCT GACTCTTTTC 
ACAGTGTCTA CAGGAGAAGG GTGGCCAACG GTACTGAAGC ATTCGGTGGA TGCAACATAT 
GAAGAACAGG GTCCAAGTCC TGGCTTCCGG ATGGAACTGT CAATCTTCTA CGTGGTCTAC 
TTTGTTGTGT TTCCCTTTTT CTTCGTCAAT ATCTTTGTGG CATTGATCAT CATCACCTTC 
CAGGAGCAGG GAGACAAAGT GATGTCAGAA TGCAGCTTGG AGAAGAATGA GAGGGCTTGC 
ATAGACTTTG CCATCAGTGC CAAGCCCCTG ACCCGTTACA TGCCTCAGAA CAAGCAGTCC 
TTCCAGTACA AGACATGGAC CTTTGTGGTG TCTCCACCCT TTGAGTATTT CATCATGGCC 
ATGATCGCCC TCAACACGGC CGTCTTGATG ATGAAGTTCT ATGGTGCCCC CTATGAGTAT 
GAAATGATGC TGAAGGGCTT GAATATTGTA TTCACTTCAA TGTTCTCTAT GGAGTGTGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAT TATTTCAGAG ATGCCTGGAA TGTTTTTGAT 
TTTGTAACAG TATTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GAACAACTTC 
ATCAATCTCA GCTTCTTGCG GCTCTTTCGG GCAGCCCGAC TCATTAAACT GCTTCGCCAG 
GGATATACTA TCCGAATTCT CCTGTGGACC TTTGTTCAGT CCTTCAAGGC TCTGCCGTAT 
GTCTGCTTAC TCATCGCCAT GCTCTTCTTC ATCTACGCCA TCATTGGCAT GCAGGTCTTT 
GGAAACATTG CTCTTGATGA TGAAACGAAT ATCAACCGGC ACAACAATTT CCGGACATTC 
CTGCAAGCCC TGATGCTGCT GTTCAGGAGT GCTACAGGGG AAGCCTGGCA TGAGATCATG 
TTGTCATGTC TTAGTGACCG TCCCTGTGAT CCTGAAACCA AACTAAAAGA TGAATGTGGG 
AGTGACTTTG CTTACTTTTA CTTCGTCTCC TTCATTTTCC TCTGCTCCTT TCTGATGCTG 
AATCTCTTTG TGGCAGTGAT TATGGATAAT TTTGAGTACC TGACTCGAGA CTCTTCCATC 
CTAGGACCTC ATCACCTGGA CGAATTTATT CGGGTCTGGG CTGAATATGA CCCAGCTGCA 
TGTGGGCGCA TCAGTTACAG TGACATGTTT GAGATGCTGA AACACATGTC CCCACCCCTC 
GGCCTGGGAA AGAAATGCCC TGCTCGAGTT GCCTATAAGC GCCTGGTGCG AATGAACATG 
CCCATCTCCA ATGAGGACAT GACTGTGCAC TTCACTTCCA CCTTGATGGC CCTCATCCGG 
ACGGCTCTGG ACATCAAGCT TGCACCAGCG GGGATGAAAC AGCATCAGTG TGATGCAGAA 
CTGAGGAAAG AGATTGCATC TGTTTGGGCT AATCTGCCCC AGAAGACACT GGACTTACTG 
GTGCCTCCAC ATAAACCTGA TGAGATGACT GTAGGAAAAG TCTACGCAGC TCTGATGATT 
TTTGACTTCT ACAAGCAGAA CAAAAACACC CGAGATCAAG GTCACCAGCC TCCGACAGGT 
CCTGTGTCTC TGTTCCATAC CCAAGGCCAA CCAGCTGTGC TCCGAGGTGC TAGAGTGTTC 
TTGAAACAGA AAAGTTCAAC CTCCCTAAGC AATGGTGGAG CCTTACCACA CCCAGGGAGT 
GGTATCAAGG AGTCTGTCTC ATGGGGCACC CAGCGCACCC AGGATATCTG CTATGAGACC 
AGGATGCCCC TGGAACGTGG TCATTCTGCA GAAATTCCTG TGGGACAACC TAGCAAACAA 
GCTGTGGAGA TGCAAGAAAT GGCTCAGGAT GCCACCAGTG GAGAACCCCA GCCAGGGCTG 
GAAAGTCAGG GCCGAGCGGC CTCCATGCCC CGCCTGGCTG CAGAGATGCA GAGGAGCCAA 
GCGCGATCGC CTGGGAGTTA CCTAGCACCC ATTCCAGACA CAAGCCCCAT GAAGCGTTCC 
ATCTCCACCT TGGCCCCACA GCGCCCACAT GGCTCACACC TTTATGAATA TGGTCTGGAA 
CGCGTATCCT CAGGCCAGCC TCAGCATCAT CACCATCATC GCTGTCACCG GCGCAGGGAT 
AAGAAGCAGA AGTCTTTGGA CAAATGCACT AGTCAGTCAG CAGATGGAGA TGGGGCACCA 
AACCATAGTG TGGGGATGGC AGCTCCATCA GGGGAAGGTC CAGCCCCATG TAAGCATGAG 
AGGAAGCAAG AAAGAGGGCG ATCCCAGGAG CGGAAGCAGC TCTCTTCCTC CTCTTCAGAG 
AAGCAGCGTT TCTATTCTTG TGACCGATTT GGGAGCCGGG ACCCATCACA GCCCAAATCC 
TCCAATGGAA GTCGTCCTAC ATCACCTCCT GGGCAGGAGC CAGGTCCCCA CAGACAGGGT 
AGTGGTTCAG TTAATGGGAG CCCCTTGCTG TCAACATCTG GTGCTAGTAC ACCGGGCCGT 
GGTGGGCGGA GGCAGCTCCC CCAGACACCT CTGACCCCCC GTCCCAGCAT CACATACAAG 
ACTGCGAACT CCTCGCCCGT CCACTTCACC GGTTTCCAGA CCAGCCTGCC CACCTTCTCC 
CCGGGACGGC TCAGTCGAGG CCTTTCTGAG CACAACACCC TGCTCCAGAG GGATTCTCTG 
AGCCAGCCGC CAACCCCAGT GTCTCGGATT GGCTCGGACC CATACCTGGG GCACCGTGAC 
CACAGCGATT CCTCGTACCG GGCCCTGCCC GAGGACACAC TCACTTTTGA GGAAGCCGTC 
GCCACCAACT CGGGCCGCTC ATCGAGGACT TCCTACGTGT CCTCTCTAAC ATCCCAGTCC 
CACCATCTCC GTCGTGTGCC CAATGGTTAC CACTGCAGTC TGGGGCTCAG CTCCGGCCCC 
GGGACTGGCG GTGGCAGGGC CCGGCAGAGT TATTATCACC AAACCGACGA AGATGACTGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951857.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807717.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X9, mRNA.

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CloneID	OSa224807
Clone ID Related Accession (Same CDS sequence)	XM_031951857.1
Accession Version	XM_031951857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCAGGAAA AGGGAAAGAC 
ATCAGCACAA TCAAGTCACT GCGAGTACTC CGAGTCCTGA GACCTCTGAA GACCATCAAA 
CGTTTGCCAA AACTAAAGGC TGTGTTTGAC TGTGTGGTAA ACTCCCTGAA GAATGTCCTC 
AATATCCTCA TTGTCTACAT GCTCTTCATG TTCATCTTTG CCGTCATTGC TGTGCAGCTC 
TTCAAAGGAA AATTCTTCTA TTGCACAGAT GAATCCAAAG AGCTTCAGAG GGATTGCAGG 
GGTCAGTATT TGGATTATGA AAAGGACGAA GTGGAGGCTC AACCCAGACA ATGGAAGAAA 
TATGACTTTC ACTATGACAA TGTGCTTTGG GCTTTGCTGA CTCTTTTCAC AGTGTCTACA 
GGAGAAGGGT GGCCAACGGT ACTGAAGCAT TCGGTGGATG CAACATATGA AGAACAGGGT 
CCAAGTCCTG GCTTCCGGAT GGAACTGTCA ATCTTCTACG TGGTCTACTT TGTTGTGTTT 
CCCTTTTTCT TCGTCAATAT CTTTGTGGCA TTGATCATCA TCACCTTCCA GGAGCAGGGA 
GACAAAGTGA TGTCAGAATG CAGCTTGGAG AAGAATGAGA GGGCTTGCAT AGACTTTGCC 
ATCAGTGCCA AGCCCCTGAC CCGTTACATG CCTCAGAACA AGCAGTCCTT CCAGTACAAG 
ACATGGACCT TTGTGGTGTC TCCACCCTTT GAGTATTTCA TCATGGCCAT GATCGCCCTC 
AACACGGCCG TCTTGATGAT GAAGTTCTAT GGTGCCCCCT ATGAGTATGA AATGATGCTG 
AAGGGCTTGA ATATTGTATT CACTTCAATG TTCTCTATGG AGTGTGTGCT GAAGATCATC 
GCCTTTGGGG TGCTGAATTA TTTCAGAGAT GCCTGGAATG TTTTTGATTT TGTAACAGTA 
TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGG AAACGAACAA CTTCATCAAT 
CTCAGCTTCT TGCGGCTCTT TCGGGCAGCC CGACTCATTA AACTGCTTCG CCAGGGATAT 
ACTATCCGAA TTCTCCTGTG GACCTTTGTT CAGTCCTTCA AGGCTCTGCC GTATGTCTGC 
TTACTCATCG CCATGCTCTT CTTCATCTAC GCCATCATTG GCATGCAGGT CTTTGGAAAC 
ATTGCTCTTG ATGATGAAAC GAATATCAAC CGGCACAACA ATTTCCGGAC ATTCCTGCAA 
GCCCTGATGC TGCTGTTCAG GAGTGCTACA GGGGAAGCCT GGCATGAGAT CATGTTGTCA 
TGTCTTAGTG ACCGTCCCTG TGATCCTGAA ACCAAACTAA AAGATGAATG TGGGAGTGAC 
TTTGCTTACT TTTACTTCGT CTCCTTCATT TTCCTCTGCT CCTTTCTGAT GCTGAATCTC 
TTTGTGGCAG TGATTATGGA TAATTTTGAG TACCTGACTC GAGACTCTTC CATCCTAGGA 
CCTCATCACC TGGACGAATT TATTCGGGTC TGGGCTGAAT ATGACCCAGC TGCATGTGGG 
CGCATCAGTT ACAGTGACAT GTTTGAGATG CTGAAACACA TGTCCCCACC CCTCGGCCTG 
GGAAAGAAAT GCCCTGCTCG AGTTGCCTAT AAGCGCCTGG TGCGAATGAA CATGCCCATC 
TCCAATGAGG ACATGACTGT GCACTTCACT TCCACCTTGA TGGCCCTCAT CCGGACGGCT 
CTGGACATCA AGCTTGCACC AGCGGGGATG AAACAGCATC AGTGTGATGC AGAACTGAGG 
AAAGAGATTG CATCTGTTTG GGCTAATCTG CCCCAGAAGA CACTGGACTT ACTGGTGCCT 
CCACATAAAC CTGATGAGAT GACTGTAGGA AAAGTCTACG CAGCTCTGAT GATTTTTGAC 
TTCTACAAGC AGAACAAAAA CACCCGAGAT CAAGGTCACC AGCCTCCGAC AGGTCCTGTG 
TCTCTGTTCC ATACCCAAGG CCAACCAGCT GTGCTCCGAG GTGCTAGAGT GTTCTTGAAA 
CAGAAAAGTT CAACCTCCCT AAGCAATGGT GGAGCCTTAC CACACCCAGG GAGTGGTATC 
AAGGAGTCTG TCTCATGGGG CACCCAGCGC ACCCAGGATA TCTGCTATGA GACCAGGATG 
CCCCTGGAAC GTGGTCATTC TGCAGAAATT CCTGTGGGAC AACCTAGCAA ACAAGCTGTG 
GAGATGCAAG AAATGGCTCA GGATGCCACC AGTGGAGAAC CCCAGCCAGG GCTGGAAAGT 
CAGGGCCGAG CGGCCTCCAT GCCCCGCCTG GCTGCAGAGA TGCAGAGGAG CCAAGCGCGA 
TCGCCTGGGA GTTACCTAGC ACCCATTCCA GACACAAGCC CCATGAAGCG TTCCATCTCC 
ACCTTGGCCC CACAGCGCCC ACATGGCTCA CACCTTTATG AATATGGTCT GGAACGCGTA 
TCCTCAGGCC AGCCTCAGCA TCATCACCAT CATCGCTGTC ACCGGCGCAG GGATAAGAAG 
CAGAAGTCTT TGGACAAATG CACTAGTCAG TCAGCAGATG GAGATGGGGC ACCAAACCAT 
AGTGTGGGGA TGGCAGCTCC ATCAGGGGAA GGTCCAGCCC CATGTAAGCA TGAGAGGAAG 
CAAGAAAGAG GGCGATCCCA GGAGCGGAAG CAGCTCTCTT CCTCCTCTTC AGAGAAGCAG 
CGTTTCTATT CTTGTGACCG ATTTGGGAGC CGGGACCCAT CACAGCCCAA ATCCTCCAAT 
GGAAGTCGTC CTACATCACC TCCTGGGCAG GAGCCAGGTC CCCACAGACA GGGTAGTGGT 
TCAGTTAATG GGAGCCCCTT GCTGTCAACA TCTGGTGCTA GTACACCGGG CCGTGGTGGG 
CGGAGGCAGC TCCCCCAGAC ACCTCTGACC CCCCGTCCCA GCATCACATA CAAGACTGCG 
AACTCCTCGC CCGTCCACTT CACCGGTTTC CAGACCAGCC TGCCCACCTT CTCCCCGGGA 
CGGCTCAGTC GAGGCCTTTC TGAGCACAAC ACCCTGCTCC AGAGGGATTC TCTGAGCCAG 
CCGCCAACCC CAGTGTCTCG GATTGGCTCG GACCCATACC TGGGGCACCG TGACCACAGC 
GATTCCTCGT ACCGGGCCCT GCCCGAGGAC ACACTCACTT TTGAGGAAGC CGTCGCCACC 
AACTCGGGCC GCTCATCGAG GACTTCCTAC GTGTCCTCTC TAACATCCCA GTCCCACCAT 
CTCCGTCGTG TGCCCAATGG TTACCACTGC AGTCTGGGGC TCAGCTCCGG CCCCGGGACT 
GGCGGTGGCA GGGCCCGGCA GAGTTATTAT CACCAAACCG ACGAAGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807717.1
CDS	1..7080
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951857.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951857.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCAGGAAA AGGGAAAGAC 
ATCAGCACAA TCAAGTCACT GCGAGTACTC CGAGTCCTGA GACCTCTGAA GACCATCAAA 
CGTTTGCCAA AACTAAAGGC TGTGTTTGAC TGTGTGGTAA ACTCCCTGAA GAATGTCCTC 
AATATCCTCA TTGTCTACAT GCTCTTCATG TTCATCTTTG CCGTCATTGC TGTGCAGCTC 
TTCAAAGGAA AATTCTTCTA TTGCACAGAT GAATCCAAAG AGCTTCAGAG GGATTGCAGG 
GGTCAGTATT TGGATTATGA AAAGGACGAA GTGGAGGCTC AACCCAGACA ATGGAAGAAA 
TATGACTTTC ACTATGACAA TGTGCTTTGG GCTTTGCTGA CTCTTTTCAC AGTGTCTACA 
GGAGAAGGGT GGCCAACGGT ACTGAAGCAT TCGGTGGATG CAACATATGA AGAACAGGGT 
CCAAGTCCTG GCTTCCGGAT GGAACTGTCA ATCTTCTACG TGGTCTACTT TGTTGTGTTT 
CCCTTTTTCT TCGTCAATAT CTTTGTGGCA TTGATCATCA TCACCTTCCA GGAGCAGGGA 
GACAAAGTGA TGTCAGAATG CAGCTTGGAG AAGAATGAGA GGGCTTGCAT AGACTTTGCC 
ATCAGTGCCA AGCCCCTGAC CCGTTACATG CCTCAGAACA AGCAGTCCTT CCAGTACAAG 
ACATGGACCT TTGTGGTGTC TCCACCCTTT GAGTATTTCA TCATGGCCAT GATCGCCCTC 
AACACGGCCG TCTTGATGAT GAAGTTCTAT GGTGCCCCCT ATGAGTATGA AATGATGCTG 
AAGGGCTTGA ATATTGTATT CACTTCAATG TTCTCTATGG AGTGTGTGCT GAAGATCATC 
GCCTTTGGGG TGCTGAATTA TTTCAGAGAT GCCTGGAATG TTTTTGATTT TGTAACAGTA 
TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGG AAACGAACAA CTTCATCAAT 
CTCAGCTTCT TGCGGCTCTT TCGGGCAGCC CGACTCATTA AACTGCTTCG CCAGGGATAT 
ACTATCCGAA TTCTCCTGTG GACCTTTGTT CAGTCCTTCA AGGCTCTGCC GTATGTCTGC 
TTACTCATCG CCATGCTCTT CTTCATCTAC GCCATCATTG GCATGCAGGT CTTTGGAAAC 
ATTGCTCTTG ATGATGAAAC GAATATCAAC CGGCACAACA ATTTCCGGAC ATTCCTGCAA 
GCCCTGATGC TGCTGTTCAG GAGTGCTACA GGGGAAGCCT GGCATGAGAT CATGTTGTCA 
TGTCTTAGTG ACCGTCCCTG TGATCCTGAA ACCAAACTAA AAGATGAATG TGGGAGTGAC 
TTTGCTTACT TTTACTTCGT CTCCTTCATT TTCCTCTGCT CCTTTCTGAT GCTGAATCTC 
TTTGTGGCAG TGATTATGGA TAATTTTGAG TACCTGACTC GAGACTCTTC CATCCTAGGA 
CCTCATCACC TGGACGAATT TATTCGGGTC TGGGCTGAAT ATGACCCAGC TGCATGTGGG 
CGCATCAGTT ACAGTGACAT GTTTGAGATG CTGAAACACA TGTCCCCACC CCTCGGCCTG 
GGAAAGAAAT GCCCTGCTCG AGTTGCCTAT AAGCGCCTGG TGCGAATGAA CATGCCCATC 
TCCAATGAGG ACATGACTGT GCACTTCACT TCCACCTTGA TGGCCCTCAT CCGGACGGCT 
CTGGACATCA AGCTTGCACC AGCGGGGATG AAACAGCATC AGTGTGATGC AGAACTGAGG 
AAAGAGATTG CATCTGTTTG GGCTAATCTG CCCCAGAAGA CACTGGACTT ACTGGTGCCT 
CCACATAAAC CTGATGAGAT GACTGTAGGA AAAGTCTACG CAGCTCTGAT GATTTTTGAC 
TTCTACAAGC AGAACAAAAA CACCCGAGAT CAAGGTCACC AGCCTCCGAC AGGTCCTGTG 
TCTCTGTTCC ATACCCAAGG CCAACCAGCT GTGCTCCGAG GTGCTAGAGT GTTCTTGAAA 
CAGAAAAGTT CAACCTCCCT AAGCAATGGT GGAGCCTTAC CACACCCAGG GAGTGGTATC 
AAGGAGTCTG TCTCATGGGG CACCCAGCGC ACCCAGGATA TCTGCTATGA GACCAGGATG 
CCCCTGGAAC GTGGTCATTC TGCAGAAATT CCTGTGGGAC AACCTAGCAA ACAAGCTGTG 
GAGATGCAAG AAATGGCTCA GGATGCCACC AGTGGAGAAC CCCAGCCAGG GCTGGAAAGT 
CAGGGCCGAG CGGCCTCCAT GCCCCGCCTG GCTGCAGAGA TGCAGAGGAG CCAAGCGCGA 
TCGCCTGGGA GTTACCTAGC ACCCATTCCA GACACAAGCC CCATGAAGCG TTCCATCTCC 
ACCTTGGCCC CACAGCGCCC ACATGGCTCA CACCTTTATG AATATGGTCT GGAACGCGTA 
TCCTCAGGCC AGCCTCAGCA TCATCACCAT CATCGCTGTC ACCGGCGCAG GGATAAGAAG 
CAGAAGTCTT TGGACAAATG CACTAGTCAG TCAGCAGATG GAGATGGGGC ACCAAACCAT 
AGTGTGGGGA TGGCAGCTCC ATCAGGGGAA GGTCCAGCCC CATGTAAGCA TGAGAGGAAG 
CAAGAAAGAG GGCGATCCCA GGAGCGGAAG CAGCTCTCTT CCTCCTCTTC AGAGAAGCAG 
CGTTTCTATT CTTGTGACCG ATTTGGGAGC CGGGACCCAT CACAGCCCAA ATCCTCCAAT 
GGAAGTCGTC CTACATCACC TCCTGGGCAG GAGCCAGGTC CCCACAGACA GGGTAGTGGT 
TCAGTTAATG GGAGCCCCTT GCTGTCAACA TCTGGTGCTA GTACACCGGG CCGTGGTGGG 
CGGAGGCAGC TCCCCCAGAC ACCTCTGACC CCCCGTCCCA GCATCACATA CAAGACTGCG 
AACTCCTCGC CCGTCCACTT CACCGGTTTC CAGACCAGCC TGCCCACCTT CTCCCCGGGA 
CGGCTCAGTC GAGGCCTTTC TGAGCACAAC ACCCTGCTCC AGAGGGATTC TCTGAGCCAG 
CCGCCAACCC CAGTGTCTCG GATTGGCTCG GACCCATACC TGGGGCACCG TGACCACAGC 
GATTCCTCGT ACCGGGCCCT GCCCGAGGAC ACACTCACTT TTGAGGAAGC CGTCGCCACC 
AACTCGGGCC GCTCATCGAG GACTTCCTAC GTGTCCTCTC TAACATCCCA GTCCCACCAT 
CTCCGTCGTG TGCCCAATGG TTACCACTGC AGTCTGGGGC TCAGCTCCGG CCCCGGGACT 
GGCGGTGGCA GGGCCCGGCA GAGTTATTAT CACCAAACCG ACGAAGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951858.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807718.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.

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CloneID	OSa224808
Clone ID Related Accession (Same CDS sequence)	XM_031951858.1
Accession Version	XM_031951858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CAGGAAAAGG GAAAGACATC 
AGCACAATCA AGTCACTGCG AGTACTCCGA GTCCTGAGAC CTCTGAAGAC CATCAAACGT 
TTGCCAAAAC TAAAGGCTGT GTTTGACTGT GTGGTAAACT CCCTGAAGAA TGTCCTCAAT 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCCG TCATTGCTGT GCAGCTCTTC 
AAAGGAAAAT TCTTCTATTG CACAGATGAA TCCAAAGAGC TTCAGAGGGA TTGCAGGGGT 
CAGTATTTGG ATTATGAAAA GGACGAAGTG GAGGCTCAAC CCAGACAATG GAAGAAATAT 
GACTTTCACT ATGACAATGT GCTTTGGGCT TTGCTGACTC TTTTCACAGT GTCTACAGGA 
GAAGGGTGGC CAACGGTACT GAAGCATTCG GTGGATGCAA CATATGAAGA ACAGGGTCCA 
AGTCCTGGCT TCCGGATGGA ACTGTCAATC TTCTACGTGG TCTACTTTGT TGTGTTTCCC 
TTTTTCTTCG TCAATATCTT TGTGGCATTG ATCATCATCA CCTTCCAGGA GCAGGGAGAC 
AAAGTGATGT CAGAATGCAG CTTGGAGAAG AATGAGAGGG CTTGCATAGA CTTTGCCATC 
AGTGCCAAGC CCCTGACCCG TTACATGCCT CAGAACAAGC AGTCCTTCCA GTACAAGACA 
TGGACCTTTG TGGTGTCTCC ACCCTTTGAG TATTTCATCA TGGCCATGAT CGCCCTCAAC 
ACGGCCGTCT TGATGATGAA GTTCTATGGT GCCCCCTATG AGTATGAAAT GATGCTGAAG 
GGCTTGAATA TTGTATTCAC TTCAATGTTC TCTATGGAGT GTGTGCTGAA GATCATCGCC 
TTTGGGGTGC TGAATTATTT CAGAGATGCC TGGAATGTTT TTGATTTTGT AACAGTATTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGGAAA CGAACAACTT CATCAATCTC 
AGCTTCTTGC GGCTCTTTCG GGCAGCCCGA CTCATTAAAC TGCTTCGCCA GGGATATACT 
ATCCGAATTC TCCTGTGGAC CTTTGTTCAG TCCTTCAAGG CTCTGCCGTA TGTCTGCTTA 
CTCATCGCCA TGCTCTTCTT CATCTACGCC ATCATTGGCA TGCAGGTCTT TGGAAACATT 
GCTCTTGATG ATGAAACGAA TATCAACCGG CACAACAATT TCCGGACATT CCTGCAAGCC 
CTGATGCTGC TGTTCAGGAG TGCTACAGGG GAAGCCTGGC ATGAGATCAT GTTGTCATGT 
CTTAGTGACC GTCCCTGTGA TCCTGAAACC AAACTAAAAG ATGAATGTGG GAGTGACTTT 
GCTTACTTTT ACTTCGTCTC CTTCATTTTC CTCTGCTCCT TTCTGATGCT GAATCTCTTT 
GTGGCAGTGA TTATGGATAA TTTTGAGTAC CTGACTCGAG ACTCTTCCAT CCTAGGACCT 
CATCACCTGG ACGAATTTAT TCGGGTCTGG GCTGAATATG ACCCAGCTGC ATGTGGGCGC 
ATCAGTTACA GTGACATGTT TGAGATGCTG AAACACATGT CCCCACCCCT CGGCCTGGGA 
AAGAAATGCC CTGCTCGAGT TGCCTATAAG CGCCTGGTGC GAATGAACAT GCCCATCTCC 
AATGAGGACA TGACTGTGCA CTTCACTTCC ACCTTGATGG CCCTCATCCG GACGGCTCTG 
GACATCAAGC TTGCACCAGC GGGGATGAAA CAGCATCAGT GTGATGCAGA ACTGAGGAAA 
GAGATTGCAT CTGTTTGGGC TAATCTGCCC CAGAAGACAC TGGACTTACT GGTGCCTCCA 
CATAAACCTG ATGAGATGAC TGTAGGAAAA GTCTACGCAG CTCTGATGAT TTTTGACTTC 
TACAAGCAGA ACAAAAACAC CCGAGATCAA GGTCACCAGC CTCCGACAGG TCCTGTGTCT 
CTGTTCCATA CCCAAGGCCA ACCAGCTGTG CTCCGAGGTG CTAGAGTGTT CTTGAAACAG 
AAAAGTTCAA CCTCCCTAAG CAATGGTGGA GCCTTACCAC ACCCAGGGAG TGGTATCAAG 
GAGTCTGTCT CATGGGGCAC CCAGCGCACC CAGGATATCT GCTATGAGAC CAGGATGCCC 
CTGGAACGTG GTCATTCTGC AGAAATTCCT GTGGGACAAC CTAGCAAACA AGCTGTGGAG 
ATGCAAGAAA TGGCTCAGGA TGCCACCAGT GGAGAACCCC AGCCAGGGCT GGAAAGTCAG 
GGCCGAGCGG CCTCCATGCC CCGCCTGGCT GCAGAGATGC AGAGGAGCCA AGCGCGATCG 
CCTGGGAGTT ACCTAGCACC CATTCCAGAC ACAAGCCCCA TGAAGCGTTC CATCTCCACC 
TTGGCCCCAC AGCGCCCACA TGGCTCACAC CTTTATGAAT ATGGTCTGGA ACGCGTATCC 
TCAGGCCAGC CTCAGCATCA TCACCATCAT CGCTGTCACC GGCGCAGGGA TAAGAAGCAG 
AAGTCTTTGG ACAAATGCAC TAGTCAGTCA GCAGATGGAG ATGGGGCACC AAACCATAGT 
GTGGGGATGG CAGCTCCATC AGGGGAAGGT CCAGCCCCAT GTAAGCATGA GAGGAAGCAA 
GAAAGAGGGC GATCCCAGGA GCGGAAGCAG CTCTCTTCCT CCTCTTCAGA GAAGCAGCGT 
TTCTATTCTT GTGACCGATT TGGGAGCCGG GACCCATCAC AGCCCAAATC CTCCAATGGA 
AGTCGTCCTA CATCACCTCC TGGGCAGGAG CCAGGTCCCC ACAGACAGGG TAGTGGTTCA 
GTTAATGGGA GCCCCTTGCT GTCAACATCT GGTGCTAGTA CACCGGGCCG TGGTGGGCGG 
AGGCAGCTCC CCCAGACACC TCTGACCCCC CGTCCCAGCA TCACATACAA GACTGCGAAC 
TCCTCGCCCG TCCACTTCAC CGGTTTCCAG ACCAGCCTGC CCACCTTCTC CCCGGGACGG 
CTCAGTCGAG GCCTTTCTGA GCACAACACC CTGCTCCAGA GGGATTCTCT GAGCCAGCCG 
CCAACCCCAG TGTCTCGGAT TGGCTCGGAC CCATACCTGG GGCACCGTGA CCACAGCGAT 
TCCTCGTACC GGGCCCTGCC CGAGGACACA CTCACTTTTG AGGAAGCCGT CGCCACCAAC 
TCGGGCCGCT CATCGAGGAC TTCCTACGTG TCCTCTCTAA CATCCCAGTC CCACCATCTC 
CGTCGTGTGC CCAATGGTTA CCACTGCAGT CTGGGGCTCA GCTCCGGCCC CGGGACTGGC 
GGTGGCAGGG CCCGGCAGAG TTATTATCAC CAAACCGACG AAGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807718.1
CDS	1..7077
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951858.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951858.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CAGGAAAAGG GAAAGACATC 
AGCACAATCA AGTCACTGCG AGTACTCCGA GTCCTGAGAC CTCTGAAGAC CATCAAACGT 
TTGCCAAAAC TAAAGGCTGT GTTTGACTGT GTGGTAAACT CCCTGAAGAA TGTCCTCAAT 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCCG TCATTGCTGT GCAGCTCTTC 
AAAGGAAAAT TCTTCTATTG CACAGATGAA TCCAAAGAGC TTCAGAGGGA TTGCAGGGGT 
CAGTATTTGG ATTATGAAAA GGACGAAGTG GAGGCTCAAC CCAGACAATG GAAGAAATAT 
GACTTTCACT ATGACAATGT GCTTTGGGCT TTGCTGACTC TTTTCACAGT GTCTACAGGA 
GAAGGGTGGC CAACGGTACT GAAGCATTCG GTGGATGCAA CATATGAAGA ACAGGGTCCA 
AGTCCTGGCT TCCGGATGGA ACTGTCAATC TTCTACGTGG TCTACTTTGT TGTGTTTCCC 
TTTTTCTTCG TCAATATCTT TGTGGCATTG ATCATCATCA CCTTCCAGGA GCAGGGAGAC 
AAAGTGATGT CAGAATGCAG CTTGGAGAAG AATGAGAGGG CTTGCATAGA CTTTGCCATC 
AGTGCCAAGC CCCTGACCCG TTACATGCCT CAGAACAAGC AGTCCTTCCA GTACAAGACA 
TGGACCTTTG TGGTGTCTCC ACCCTTTGAG TATTTCATCA TGGCCATGAT CGCCCTCAAC 
ACGGCCGTCT TGATGATGAA GTTCTATGGT GCCCCCTATG AGTATGAAAT GATGCTGAAG 
GGCTTGAATA TTGTATTCAC TTCAATGTTC TCTATGGAGT GTGTGCTGAA GATCATCGCC 
TTTGGGGTGC TGAATTATTT CAGAGATGCC TGGAATGTTT TTGATTTTGT AACAGTATTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGGAAA CGAACAACTT CATCAATCTC 
AGCTTCTTGC GGCTCTTTCG GGCAGCCCGA CTCATTAAAC TGCTTCGCCA GGGATATACT 
ATCCGAATTC TCCTGTGGAC CTTTGTTCAG TCCTTCAAGG CTCTGCCGTA TGTCTGCTTA 
CTCATCGCCA TGCTCTTCTT CATCTACGCC ATCATTGGCA TGCAGGTCTT TGGAAACATT 
GCTCTTGATG ATGAAACGAA TATCAACCGG CACAACAATT TCCGGACATT CCTGCAAGCC 
CTGATGCTGC TGTTCAGGAG TGCTACAGGG GAAGCCTGGC ATGAGATCAT GTTGTCATGT 
CTTAGTGACC GTCCCTGTGA TCCTGAAACC AAACTAAAAG ATGAATGTGG GAGTGACTTT 
GCTTACTTTT ACTTCGTCTC CTTCATTTTC CTCTGCTCCT TTCTGATGCT GAATCTCTTT 
GTGGCAGTGA TTATGGATAA TTTTGAGTAC CTGACTCGAG ACTCTTCCAT CCTAGGACCT 
CATCACCTGG ACGAATTTAT TCGGGTCTGG GCTGAATATG ACCCAGCTGC ATGTGGGCGC 
ATCAGTTACA GTGACATGTT TGAGATGCTG AAACACATGT CCCCACCCCT CGGCCTGGGA 
AAGAAATGCC CTGCTCGAGT TGCCTATAAG CGCCTGGTGC GAATGAACAT GCCCATCTCC 
AATGAGGACA TGACTGTGCA CTTCACTTCC ACCTTGATGG CCCTCATCCG GACGGCTCTG 
GACATCAAGC TTGCACCAGC GGGGATGAAA CAGCATCAGT GTGATGCAGA ACTGAGGAAA 
GAGATTGCAT CTGTTTGGGC TAATCTGCCC CAGAAGACAC TGGACTTACT GGTGCCTCCA 
CATAAACCTG ATGAGATGAC TGTAGGAAAA GTCTACGCAG CTCTGATGAT TTTTGACTTC 
TACAAGCAGA ACAAAAACAC CCGAGATCAA GGTCACCAGC CTCCGACAGG TCCTGTGTCT 
CTGTTCCATA CCCAAGGCCA ACCAGCTGTG CTCCGAGGTG CTAGAGTGTT CTTGAAACAG 
AAAAGTTCAA CCTCCCTAAG CAATGGTGGA GCCTTACCAC ACCCAGGGAG TGGTATCAAG 
GAGTCTGTCT CATGGGGCAC CCAGCGCACC CAGGATATCT GCTATGAGAC CAGGATGCCC 
CTGGAACGTG GTCATTCTGC AGAAATTCCT GTGGGACAAC CTAGCAAACA AGCTGTGGAG 
ATGCAAGAAA TGGCTCAGGA TGCCACCAGT GGAGAACCCC AGCCAGGGCT GGAAAGTCAG 
GGCCGAGCGG CCTCCATGCC CCGCCTGGCT GCAGAGATGC AGAGGAGCCA AGCGCGATCG 
CCTGGGAGTT ACCTAGCACC CATTCCAGAC ACAAGCCCCA TGAAGCGTTC CATCTCCACC 
TTGGCCCCAC AGCGCCCACA TGGCTCACAC CTTTATGAAT ATGGTCTGGA ACGCGTATCC 
TCAGGCCAGC CTCAGCATCA TCACCATCAT CGCTGTCACC GGCGCAGGGA TAAGAAGCAG 
AAGTCTTTGG ACAAATGCAC TAGTCAGTCA GCAGATGGAG ATGGGGCACC AAACCATAGT 
GTGGGGATGG CAGCTCCATC AGGGGAAGGT CCAGCCCCAT GTAAGCATGA GAGGAAGCAA 
GAAAGAGGGC GATCCCAGGA GCGGAAGCAG CTCTCTTCCT CCTCTTCAGA GAAGCAGCGT 
TTCTATTCTT GTGACCGATT TGGGAGCCGG GACCCATCAC AGCCCAAATC CTCCAATGGA 
AGTCGTCCTA CATCACCTCC TGGGCAGGAG CCAGGTCCCC ACAGACAGGG TAGTGGTTCA 
GTTAATGGGA GCCCCTTGCT GTCAACATCT GGTGCTAGTA CACCGGGCCG TGGTGGGCGG 
AGGCAGCTCC CCCAGACACC TCTGACCCCC CGTCCCAGCA TCACATACAA GACTGCGAAC 
TCCTCGCCCG TCCACTTCAC CGGTTTCCAG ACCAGCCTGC CCACCTTCTC CCCGGGACGG 
CTCAGTCGAG GCCTTTCTGA GCACAACACC CTGCTCCAGA GGGATTCTCT GAGCCAGCCG 
CCAACCCCAG TGTCTCGGAT TGGCTCGGAC CCATACCTGG GGCACCGTGA CCACAGCGAT 
TCCTCGTACC GGGCCCTGCC CGAGGACACA CTCACTTTTG AGGAAGCCGT CGCCACCAAC 
TCGGGCCGCT CATCGAGGAC TTCCTACGTG TCCTCTCTAA CATCCCAGTC CCACCATCTC 
CGTCGTGTGC CCAATGGTTA CCACTGCAGT CTGGGGCTCA GCTCCGGCCC CGGGACTGGC 
GGTGGCAGGG CCCGGCAGAG TTATTATCAC CAAACCGACG AAGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951859.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807719.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X11, mRNA.

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CloneID	OSa224809
Clone ID Related Accession (Same CDS sequence)	XM_031951859.1
Accession Version	XM_031951859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCAGGAAA AGGGAAAGAC 
ATCAGCACAA TCAAGTCACT GCGAGTACTC CGAGTCCTGA GACCTCTGAA GACCATCAAA 
CGTTTGCCAA AACTAAAGGC TGTGTTTGAC TGTGTGGTAA ACTCCCTGAA GAATGTCCTC 
AATATCCTCA TTGTCTACAT GCTCTTCATG TTCATCTTTG CCGTCATTGC TGTGCAGCTC 
TTCAAAGGAA AATTCTTCTA TTGCACAGAT GAATCCAAAG AGCTTCAGAG GGATTGCAGG 
GGTCAGTATT TGGATTATGA AAAGGACGAA GTGGAGGCTC AACCCAGACA ATGGAAGAAA 
TATGACTTTC ACTATGACAA TGTGCTTTGG GCTTTGCTGA CTCTTTTCAC AGTGTCTACA 
GGAGAAGGGT GGCCAACGGT ACTGAAGCAT TCGGTGGATG CAACATATGA AGAACAGGGT 
CCAAGTCCTG GCTTCCGGAT GGAACTGTCA ATCTTCTACG TGGTCTACTT TGTTGTGTTT 
CCCTTTTTCT TCGTCAATAT CTTTGTGGCA TTGATCATCA TCACCTTCCA GGAGCAGGGA 
GACAAAGTGA TGTCAGAATG CAGCTTGGAG AAGAATGAGA GGGCTTGCAT AGACTTTGCC 
ATCAGTGCCA AGCCCCTGAC CCGTTACATG CCTCAGAACA AGCAGTCCTT CCAGTACAAG 
ACATGGACCT TTGTGGTGTC TCCACCCTTT GAGTATTTCA TCATGGCCAT GATCGCCCTC 
AACACGGCCG TCTTGATGAT GAAGTTCTAT GGTGCCCCCT ATGAGTATGA AATGATGCTG 
AAGGGCTTGA ATATTGTATT CACTTCAATG TTCTCTATGG AGTGTGTGCT GAAGATCATC 
GCCTTTGGGG TGCTGAATTA TTTCAGAGAT GCCTGGAATG TTTTTGATTT TGTAACAGTA 
TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGA ACAACTTCAT CAATCTCAGC 
TTCTTGCGGC TCTTTCGGGC AGCCCGACTC ATTAAACTGC TTCGCCAGGG ATATACTATC 
CGAATTCTCC TGTGGACCTT TGTTCAGTCC TTCAAGGCTC TGCCGTATGT CTGCTTACTC 
ATCGCCATGC TCTTCTTCAT CTACGCCATC ATTGGCATGC AGGTCTTTGG AAACATTGCT 
CTTGATGATG AAACGAATAT CAACCGGCAC AACAATTTCC GGACATTCCT GCAAGCCCTG 
ATGCTGCTGT TCAGGAGTGC TACAGGGGAA GCCTGGCATG AGATCATGTT GTCATGTCTT 
AGTGACCGTC CCTGTGATCC TGAAACCAAA CTAAAAGATG AATGTGGGAG TGACTTTGCT 
TACTTTTACT TCGTCTCCTT CATTTTCCTC TGCTCCTTTC TGATGCTGAA TCTCTTTGTG 
GCAGTGATTA TGGATAATTT TGAGTACCTG ACTCGAGACT CTTCCATCCT AGGACCTCAT 
CACCTGGACG AATTTATTCG GGTCTGGGCT GAATATGACC CAGCTGCATG TGGGCGCATC 
AGTTACAGTG ACATGTTTGA GATGCTGAAA CACATGTCCC CACCCCTCGG CCTGGGAAAG 
AAATGCCCTG CTCGAGTTGC CTATAAGCGC CTGGTGCGAA TGAACATGCC CATCTCCAAT 
GAGGACATGA CTGTGCACTT CACTTCCACC TTGATGGCCC TCATCCGGAC GGCTCTGGAC 
ATCAAGCTTG CACCAGCGGG GATGAAACAG CATCAGTGTG ATGCAGAACT GAGGAAAGAG 
ATTGCATCTG TTTGGGCTAA TCTGCCCCAG AAGACACTGG ACTTACTGGT GCCTCCACAT 
AAACCTGATG AGATGACTGT AGGAAAAGTC TACGCAGCTC TGATGATTTT TGACTTCTAC 
AAGCAGAACA AAAACACCCG AGATCAAGGT CACCAGCCTC CGACAGGTCC TGTGTCTCTG 
TTCCATACCC AAGGCCAACC AGCTGTGCTC CGAGGTGCTA GAGTGTTCTT GAAACAGAAA 
AGTTCAACCT CCCTAAGCAA TGGTGGAGCC TTACCACACC CAGGGAGTGG TATCAAGGAG 
TCTGTCTCAT GGGGCACCCA GCGCACCCAG GATATCTGCT ATGAGACCAG GATGCCCCTG 
GAACGTGGTC ATTCTGCAGA AATTCCTGTG GGACAACCTA GCAAACAAGC TGTGGAGATG 
CAAGAAATGG CTCAGGATGC CACCAGTGGA GAACCCCAGC CAGGGCTGGA AAGTCAGGGC 
CGAGCGGCCT CCATGCCCCG CCTGGCTGCA GAGATGCAGA GGAGCCAAGC GCGATCGCCT 
GGGAGTTACC TAGCACCCAT TCCAGACACA AGCCCCATGA AGCGTTCCAT CTCCACCTTG 
GCCCCACAGC GCCCACATGG CTCACACCTT TATGAATATG GTCTGGAACG CGTATCCTCA 
GGCCAGCCTC AGCATCATCA CCATCATCGC TGTCACCGGC GCAGGGATAA GAAGCAGAAG 
TCTTTGGACA AATGCACTAG TCAGTCAGCA GATGGAGATG GGGCACCAAA CCATAGTGTG 
GGGATGGCAG CTCCATCAGG GGAAGGTCCA GCCCCATGTA AGCATGAGAG GAAGCAAGAA 
AGAGGGCGAT CCCAGGAGCG GAAGCAGCTC TCTTCCTCCT CTTCAGAGAA GCAGCGTTTC 
TATTCTTGTG ACCGATTTGG GAGCCGGGAC CCATCACAGC CCAAATCCTC CAATGGAAGT 
CGTCCTACAT CACCTCCTGG GCAGGAGCCA GGTCCCCACA GACAGGGTAG TGGTTCAGTT 
AATGGGAGCC CCTTGCTGTC AACATCTGGT GCTAGTACAC CGGGCCGTGG TGGGCGGAGG 
CAGCTCCCCC AGACACCTCT GACCCCCCGT CCCAGCATCA CATACAAGAC TGCGAACTCC 
TCGCCCGTCC ACTTCACCGG TTTCCAGACC AGCCTGCCCA CCTTCTCCCC GGGACGGCTC 
AGTCGAGGCC TTTCTGAGCA CAACACCCTG CTCCAGAGGG ATTCTCTGAG CCAGCCGCCA 
ACCCCAGTGT CTCGGATTGG CTCGGACCCA TACCTGGGGC ACCGTGACCA CAGCGATTCC 
TCGTACCGGG CCCTGCCCGA GGACACACTC ACTTTTGAGG AAGCCGTCGC CACCAACTCG 
GGCCGCTCAT CGAGGACTTC CTACGTGTCC TCTCTAACAT CCCAGTCCCA CCATCTCCGT 
CGTGTGCCCA ATGGTTACCA CTGCAGTCTG GGGCTCAGCT CCGGCCCCGG GACTGGCGGT 
GGCAGGGCCC GGCAGAGTTA TTATCACCAA ACCGACGAAG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807719.1
CDS	1..7074
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951859.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951859.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCAGGAAA AGGGAAAGAC 
ATCAGCACAA TCAAGTCACT GCGAGTACTC CGAGTCCTGA GACCTCTGAA GACCATCAAA 
CGTTTGCCAA AACTAAAGGC TGTGTTTGAC TGTGTGGTAA ACTCCCTGAA GAATGTCCTC 
AATATCCTCA TTGTCTACAT GCTCTTCATG TTCATCTTTG CCGTCATTGC TGTGCAGCTC 
TTCAAAGGAA AATTCTTCTA TTGCACAGAT GAATCCAAAG AGCTTCAGAG GGATTGCAGG 
GGTCAGTATT TGGATTATGA AAAGGACGAA GTGGAGGCTC AACCCAGACA ATGGAAGAAA 
TATGACTTTC ACTATGACAA TGTGCTTTGG GCTTTGCTGA CTCTTTTCAC AGTGTCTACA 
GGAGAAGGGT GGCCAACGGT ACTGAAGCAT TCGGTGGATG CAACATATGA AGAACAGGGT 
CCAAGTCCTG GCTTCCGGAT GGAACTGTCA ATCTTCTACG TGGTCTACTT TGTTGTGTTT 
CCCTTTTTCT TCGTCAATAT CTTTGTGGCA TTGATCATCA TCACCTTCCA GGAGCAGGGA 
GACAAAGTGA TGTCAGAATG CAGCTTGGAG AAGAATGAGA GGGCTTGCAT AGACTTTGCC 
ATCAGTGCCA AGCCCCTGAC CCGTTACATG CCTCAGAACA AGCAGTCCTT CCAGTACAAG 
ACATGGACCT TTGTGGTGTC TCCACCCTTT GAGTATTTCA TCATGGCCAT GATCGCCCTC 
AACACGGCCG TCTTGATGAT GAAGTTCTAT GGTGCCCCCT ATGAGTATGA AATGATGCTG 
AAGGGCTTGA ATATTGTATT CACTTCAATG TTCTCTATGG AGTGTGTGCT GAAGATCATC 
GCCTTTGGGG TGCTGAATTA TTTCAGAGAT GCCTGGAATG TTTTTGATTT TGTAACAGTA 
TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGA ACAACTTCAT CAATCTCAGC 
TTCTTGCGGC TCTTTCGGGC AGCCCGACTC ATTAAACTGC TTCGCCAGGG ATATACTATC 
CGAATTCTCC TGTGGACCTT TGTTCAGTCC TTCAAGGCTC TGCCGTATGT CTGCTTACTC 
ATCGCCATGC TCTTCTTCAT CTACGCCATC ATTGGCATGC AGGTCTTTGG AAACATTGCT 
CTTGATGATG AAACGAATAT CAACCGGCAC AACAATTTCC GGACATTCCT GCAAGCCCTG 
ATGCTGCTGT TCAGGAGTGC TACAGGGGAA GCCTGGCATG AGATCATGTT GTCATGTCTT 
AGTGACCGTC CCTGTGATCC TGAAACCAAA CTAAAAGATG AATGTGGGAG TGACTTTGCT 
TACTTTTACT TCGTCTCCTT CATTTTCCTC TGCTCCTTTC TGATGCTGAA TCTCTTTGTG 
GCAGTGATTA TGGATAATTT TGAGTACCTG ACTCGAGACT CTTCCATCCT AGGACCTCAT 
CACCTGGACG AATTTATTCG GGTCTGGGCT GAATATGACC CAGCTGCATG TGGGCGCATC 
AGTTACAGTG ACATGTTTGA GATGCTGAAA CACATGTCCC CACCCCTCGG CCTGGGAAAG 
AAATGCCCTG CTCGAGTTGC CTATAAGCGC CTGGTGCGAA TGAACATGCC CATCTCCAAT 
GAGGACATGA CTGTGCACTT CACTTCCACC TTGATGGCCC TCATCCGGAC GGCTCTGGAC 
ATCAAGCTTG CACCAGCGGG GATGAAACAG CATCAGTGTG ATGCAGAACT GAGGAAAGAG 
ATTGCATCTG TTTGGGCTAA TCTGCCCCAG AAGACACTGG ACTTACTGGT GCCTCCACAT 
AAACCTGATG AGATGACTGT AGGAAAAGTC TACGCAGCTC TGATGATTTT TGACTTCTAC 
AAGCAGAACA AAAACACCCG AGATCAAGGT CACCAGCCTC CGACAGGTCC TGTGTCTCTG 
TTCCATACCC AAGGCCAACC AGCTGTGCTC CGAGGTGCTA GAGTGTTCTT GAAACAGAAA 
AGTTCAACCT CCCTAAGCAA TGGTGGAGCC TTACCACACC CAGGGAGTGG TATCAAGGAG 
TCTGTCTCAT GGGGCACCCA GCGCACCCAG GATATCTGCT ATGAGACCAG GATGCCCCTG 
GAACGTGGTC ATTCTGCAGA AATTCCTGTG GGACAACCTA GCAAACAAGC TGTGGAGATG 
CAAGAAATGG CTCAGGATGC CACCAGTGGA GAACCCCAGC CAGGGCTGGA AAGTCAGGGC 
CGAGCGGCCT CCATGCCCCG CCTGGCTGCA GAGATGCAGA GGAGCCAAGC GCGATCGCCT 
GGGAGTTACC TAGCACCCAT TCCAGACACA AGCCCCATGA AGCGTTCCAT CTCCACCTTG 
GCCCCACAGC GCCCACATGG CTCACACCTT TATGAATATG GTCTGGAACG CGTATCCTCA 
GGCCAGCCTC AGCATCATCA CCATCATCGC TGTCACCGGC GCAGGGATAA GAAGCAGAAG 
TCTTTGGACA AATGCACTAG TCAGTCAGCA GATGGAGATG GGGCACCAAA CCATAGTGTG 
GGGATGGCAG CTCCATCAGG GGAAGGTCCA GCCCCATGTA AGCATGAGAG GAAGCAAGAA 
AGAGGGCGAT CCCAGGAGCG GAAGCAGCTC TCTTCCTCCT CTTCAGAGAA GCAGCGTTTC 
TATTCTTGTG ACCGATTTGG GAGCCGGGAC CCATCACAGC CCAAATCCTC CAATGGAAGT 
CGTCCTACAT CACCTCCTGG GCAGGAGCCA GGTCCCCACA GACAGGGTAG TGGTTCAGTT 
AATGGGAGCC CCTTGCTGTC AACATCTGGT GCTAGTACAC CGGGCCGTGG TGGGCGGAGG 
CAGCTCCCCC AGACACCTCT GACCCCCCGT CCCAGCATCA CATACAAGAC TGCGAACTCC 
TCGCCCGTCC ACTTCACCGG TTTCCAGACC AGCCTGCCCA CCTTCTCCCC GGGACGGCTC 
AGTCGAGGCC TTTCTGAGCA CAACACCCTG CTCCAGAGGG ATTCTCTGAG CCAGCCGCCA 
ACCCCAGTGT CTCGGATTGG CTCGGACCCA TACCTGGGGC ACCGTGACCA CAGCGATTCC 
TCGTACCGGG CCCTGCCCGA GGACACACTC ACTTTTGAGG AAGCCGTCGC CACCAACTCG 
GGCCGCTCAT CGAGGACTTC CTACGTGTCC TCTCTAACAT CCCAGTCCCA CCATCTCCGT 
CGTGTGCCCA ATGGTTACCA CTGCAGTCTG GGGCTCAGCT CCGGCCCCGG GACTGGCGGT 
GGCAGGGCCC GGCAGAGTTA TTATCACCAA ACCGACGAAG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951860.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807720.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X12, mRNA.

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CloneID	OSa224810
Clone ID Related Accession (Same CDS sequence)	XM_031951860.1
Accession Version	XM_031951860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CAGGAAAAGG GAAAGACATC 
AGCACAATCA AGTCACTGCG AGTACTCCGA GTCCTGAGAC CTCTGAAGAC CATCAAACGT 
TTGCCAAAAC TAAAGGCTGT GTTTGACTGT GTGGTAAACT CCCTGAAGAA TGTCCTCAAT 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCCG TCATTGCTGT GCAGCTCTTC 
AAAGGAAAAT TCTTCTATTG CACAGATGAA TCCAAAGAGC TTCAGAGGGA TTGCAGGGGT 
CAGTATTTGG ATTATGAAAA GGACGAAGTG GAGGCTCAAC CCAGACAATG GAAGAAATAT 
GACTTTCACT ATGACAATGT GCTTTGGGCT TTGCTGACTC TTTTCACAGT GTCTACAGGA 
GAAGGGTGGC CAACGGTACT GAAGCATTCG GTGGATGCAA CATATGAAGA ACAGGGTCCA 
AGTCCTGGCT TCCGGATGGA ACTGTCAATC TTCTACGTGG TCTACTTTGT TGTGTTTCCC 
TTTTTCTTCG TCAATATCTT TGTGGCATTG ATCATCATCA CCTTCCAGGA GCAGGGAGAC 
AAAGTGATGT CAGAATGCAG CTTGGAGAAG AATGAGAGGG CTTGCATAGA CTTTGCCATC 
AGTGCCAAGC CCCTGACCCG TTACATGCCT CAGAACAAGC AGTCCTTCCA GTACAAGACA 
TGGACCTTTG TGGTGTCTCC ACCCTTTGAG TATTTCATCA TGGCCATGAT CGCCCTCAAC 
ACGGCCGTCT TGATGATGAA GTTCTATGGT GCCCCCTATG AGTATGAAAT GATGCTGAAG 
GGCTTGAATA TTGTATTCAC TTCAATGTTC TCTATGGAGT GTGTGCTGAA GATCATCGCC 
TTTGGGGTGC TGAATTATTT CAGAGATGCC TGGAATGTTT TTGATTTTGT AACAGTATTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGAACA ACTTCATCAA TCTCAGCTTC 
TTGCGGCTCT TTCGGGCAGC CCGACTCATT AAACTGCTTC GCCAGGGATA TACTATCCGA 
ATTCTCCTGT GGACCTTTGT TCAGTCCTTC AAGGCTCTGC CGTATGTCTG CTTACTCATC 
GCCATGCTCT TCTTCATCTA CGCCATCATT GGCATGCAGG TCTTTGGAAA CATTGCTCTT 
GATGATGAAA CGAATATCAA CCGGCACAAC AATTTCCGGA CATTCCTGCA AGCCCTGATG 
CTGCTGTTCA GGAGTGCTAC AGGGGAAGCC TGGCATGAGA TCATGTTGTC ATGTCTTAGT 
GACCGTCCCT GTGATCCTGA AACCAAACTA AAAGATGAAT GTGGGAGTGA CTTTGCTTAC 
TTTTACTTCG TCTCCTTCAT TTTCCTCTGC TCCTTTCTGA TGCTGAATCT CTTTGTGGCA 
GTGATTATGG ATAATTTTGA GTACCTGACT CGAGACTCTT CCATCCTAGG ACCTCATCAC 
CTGGACGAAT TTATTCGGGT CTGGGCTGAA TATGACCCAG CTGCATGTGG GCGCATCAGT 
TACAGTGACA TGTTTGAGAT GCTGAAACAC ATGTCCCCAC CCCTCGGCCT GGGAAAGAAA 
TGCCCTGCTC GAGTTGCCTA TAAGCGCCTG GTGCGAATGA ACATGCCCAT CTCCAATGAG 
GACATGACTG TGCACTTCAC TTCCACCTTG ATGGCCCTCA TCCGGACGGC TCTGGACATC 
AAGCTTGCAC CAGCGGGGAT GAAACAGCAT CAGTGTGATG CAGAACTGAG GAAAGAGATT 
GCATCTGTTT GGGCTAATCT GCCCCAGAAG ACACTGGACT TACTGGTGCC TCCACATAAA 
CCTGATGAGA TGACTGTAGG AAAAGTCTAC GCAGCTCTGA TGATTTTTGA CTTCTACAAG 
CAGAACAAAA ACACCCGAGA TCAAGGTCAC CAGCCTCCGA CAGGTCCTGT GTCTCTGTTC 
CATACCCAAG GCCAACCAGC TGTGCTCCGA GGTGCTAGAG TGTTCTTGAA ACAGAAAAGT 
TCAACCTCCC TAAGCAATGG TGGAGCCTTA CCACACCCAG GGAGTGGTAT CAAGGAGTCT 
GTCTCATGGG GCACCCAGCG CACCCAGGAT ATCTGCTATG AGACCAGGAT GCCCCTGGAA 
CGTGGTCATT CTGCAGAAAT TCCTGTGGGA CAACCTAGCA AACAAGCTGT GGAGATGCAA 
GAAATGGCTC AGGATGCCAC CAGTGGAGAA CCCCAGCCAG GGCTGGAAAG TCAGGGCCGA 
GCGGCCTCCA TGCCCCGCCT GGCTGCAGAG ATGCAGAGGA GCCAAGCGCG ATCGCCTGGG 
AGTTACCTAG CACCCATTCC AGACACAAGC CCCATGAAGC GTTCCATCTC CACCTTGGCC 
CCACAGCGCC CACATGGCTC ACACCTTTAT GAATATGGTC TGGAACGCGT ATCCTCAGGC 
CAGCCTCAGC ATCATCACCA TCATCGCTGT CACCGGCGCA GGGATAAGAA GCAGAAGTCT 
TTGGACAAAT GCACTAGTCA GTCAGCAGAT GGAGATGGGG CACCAAACCA TAGTGTGGGG 
ATGGCAGCTC CATCAGGGGA AGGTCCAGCC CCATGTAAGC ATGAGAGGAA GCAAGAAAGA 
GGGCGATCCC AGGAGCGGAA GCAGCTCTCT TCCTCCTCTT CAGAGAAGCA GCGTTTCTAT 
TCTTGTGACC GATTTGGGAG CCGGGACCCA TCACAGCCCA AATCCTCCAA TGGAAGTCGT 
CCTACATCAC CTCCTGGGCA GGAGCCAGGT CCCCACAGAC AGGGTAGTGG TTCAGTTAAT 
GGGAGCCCCT TGCTGTCAAC ATCTGGTGCT AGTACACCGG GCCGTGGTGG GCGGAGGCAG 
CTCCCCCAGA CACCTCTGAC CCCCCGTCCC AGCATCACAT ACAAGACTGC GAACTCCTCG 
CCCGTCCACT TCACCGGTTT CCAGACCAGC CTGCCCACCT TCTCCCCGGG ACGGCTCAGT 
CGAGGCCTTT CTGAGCACAA CACCCTGCTC CAGAGGGATT CTCTGAGCCA GCCGCCAACC 
CCAGTGTCTC GGATTGGCTC GGACCCATAC CTGGGGCACC GTGACCACAG CGATTCCTCG 
TACCGGGCCC TGCCCGAGGA CACACTCACT TTTGAGGAAG CCGTCGCCAC CAACTCGGGC 
CGCTCATCGA GGACTTCCTA CGTGTCCTCT CTAACATCCC AGTCCCACCA TCTCCGTCGT 
GTGCCCAATG GTTACCACTG CAGTCTGGGG CTCAGCTCCG GCCCCGGGAC TGGCGGTGGC 
AGGGCCCGGC AGAGTTATTA TCACCAAACC GACGAAGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807720.1
CDS	1..7071
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQRSQARSPGSYLAPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951860.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951860.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CAGGAAAAGG GAAAGACATC 
AGCACAATCA AGTCACTGCG AGTACTCCGA GTCCTGAGAC CTCTGAAGAC CATCAAACGT 
TTGCCAAAAC TAAAGGCTGT GTTTGACTGT GTGGTAAACT CCCTGAAGAA TGTCCTCAAT 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCCG TCATTGCTGT GCAGCTCTTC 
AAAGGAAAAT TCTTCTATTG CACAGATGAA TCCAAAGAGC TTCAGAGGGA TTGCAGGGGT 
CAGTATTTGG ATTATGAAAA GGACGAAGTG GAGGCTCAAC CCAGACAATG GAAGAAATAT 
GACTTTCACT ATGACAATGT GCTTTGGGCT TTGCTGACTC TTTTCACAGT GTCTACAGGA 
GAAGGGTGGC CAACGGTACT GAAGCATTCG GTGGATGCAA CATATGAAGA ACAGGGTCCA 
AGTCCTGGCT TCCGGATGGA ACTGTCAATC TTCTACGTGG TCTACTTTGT TGTGTTTCCC 
TTTTTCTTCG TCAATATCTT TGTGGCATTG ATCATCATCA CCTTCCAGGA GCAGGGAGAC 
AAAGTGATGT CAGAATGCAG CTTGGAGAAG AATGAGAGGG CTTGCATAGA CTTTGCCATC 
AGTGCCAAGC CCCTGACCCG TTACATGCCT CAGAACAAGC AGTCCTTCCA GTACAAGACA 
TGGACCTTTG TGGTGTCTCC ACCCTTTGAG TATTTCATCA TGGCCATGAT CGCCCTCAAC 
ACGGCCGTCT TGATGATGAA GTTCTATGGT GCCCCCTATG AGTATGAAAT GATGCTGAAG 
GGCTTGAATA TTGTATTCAC TTCAATGTTC TCTATGGAGT GTGTGCTGAA GATCATCGCC 
TTTGGGGTGC TGAATTATTT CAGAGATGCC TGGAATGTTT TTGATTTTGT AACAGTATTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGAACA ACTTCATCAA TCTCAGCTTC 
TTGCGGCTCT TTCGGGCAGC CCGACTCATT AAACTGCTTC GCCAGGGATA TACTATCCGA 
ATTCTCCTGT GGACCTTTGT TCAGTCCTTC AAGGCTCTGC CGTATGTCTG CTTACTCATC 
GCCATGCTCT TCTTCATCTA CGCCATCATT GGCATGCAGG TCTTTGGAAA CATTGCTCTT 
GATGATGAAA CGAATATCAA CCGGCACAAC AATTTCCGGA CATTCCTGCA AGCCCTGATG 
CTGCTGTTCA GGAGTGCTAC AGGGGAAGCC TGGCATGAGA TCATGTTGTC ATGTCTTAGT 
GACCGTCCCT GTGATCCTGA AACCAAACTA AAAGATGAAT GTGGGAGTGA CTTTGCTTAC 
TTTTACTTCG TCTCCTTCAT TTTCCTCTGC TCCTTTCTGA TGCTGAATCT CTTTGTGGCA 
GTGATTATGG ATAATTTTGA GTACCTGACT CGAGACTCTT CCATCCTAGG ACCTCATCAC 
CTGGACGAAT TTATTCGGGT CTGGGCTGAA TATGACCCAG CTGCATGTGG GCGCATCAGT 
TACAGTGACA TGTTTGAGAT GCTGAAACAC ATGTCCCCAC CCCTCGGCCT GGGAAAGAAA 
TGCCCTGCTC GAGTTGCCTA TAAGCGCCTG GTGCGAATGA ACATGCCCAT CTCCAATGAG 
GACATGACTG TGCACTTCAC TTCCACCTTG ATGGCCCTCA TCCGGACGGC TCTGGACATC 
AAGCTTGCAC CAGCGGGGAT GAAACAGCAT CAGTGTGATG CAGAACTGAG GAAAGAGATT 
GCATCTGTTT GGGCTAATCT GCCCCAGAAG ACACTGGACT TACTGGTGCC TCCACATAAA 
CCTGATGAGA TGACTGTAGG AAAAGTCTAC GCAGCTCTGA TGATTTTTGA CTTCTACAAG 
CAGAACAAAA ACACCCGAGA TCAAGGTCAC CAGCCTCCGA CAGGTCCTGT GTCTCTGTTC 
CATACCCAAG GCCAACCAGC TGTGCTCCGA GGTGCTAGAG TGTTCTTGAA ACAGAAAAGT 
TCAACCTCCC TAAGCAATGG TGGAGCCTTA CCACACCCAG GGAGTGGTAT CAAGGAGTCT 
GTCTCATGGG GCACCCAGCG CACCCAGGAT ATCTGCTATG AGACCAGGAT GCCCCTGGAA 
CGTGGTCATT CTGCAGAAAT TCCTGTGGGA CAACCTAGCA AACAAGCTGT GGAGATGCAA 
GAAATGGCTC AGGATGCCAC CAGTGGAGAA CCCCAGCCAG GGCTGGAAAG TCAGGGCCGA 
GCGGCCTCCA TGCCCCGCCT GGCTGCAGAG ATGCAGAGGA GCCAAGCGCG ATCGCCTGGG 
AGTTACCTAG CACCCATTCC AGACACAAGC CCCATGAAGC GTTCCATCTC CACCTTGGCC 
CCACAGCGCC CACATGGCTC ACACCTTTAT GAATATGGTC TGGAACGCGT ATCCTCAGGC 
CAGCCTCAGC ATCATCACCA TCATCGCTGT CACCGGCGCA GGGATAAGAA GCAGAAGTCT 
TTGGACAAAT GCACTAGTCA GTCAGCAGAT GGAGATGGGG CACCAAACCA TAGTGTGGGG 
ATGGCAGCTC CATCAGGGGA AGGTCCAGCC CCATGTAAGC ATGAGAGGAA GCAAGAAAGA 
GGGCGATCCC AGGAGCGGAA GCAGCTCTCT TCCTCCTCTT CAGAGAAGCA GCGTTTCTAT 
TCTTGTGACC GATTTGGGAG CCGGGACCCA TCACAGCCCA AATCCTCCAA TGGAAGTCGT 
CCTACATCAC CTCCTGGGCA GGAGCCAGGT CCCCACAGAC AGGGTAGTGG TTCAGTTAAT 
GGGAGCCCCT TGCTGTCAAC ATCTGGTGCT AGTACACCGG GCCGTGGTGG GCGGAGGCAG 
CTCCCCCAGA CACCTCTGAC CCCCCGTCCC AGCATCACAT ACAAGACTGC GAACTCCTCG 
CCCGTCCACT TCACCGGTTT CCAGACCAGC CTGCCCACCT TCTCCCCGGG ACGGCTCAGT 
CGAGGCCTTT CTGAGCACAA CACCCTGCTC CAGAGGGATT CTCTGAGCCA GCCGCCAACC 
CCAGTGTCTC GGATTGGCTC GGACCCATAC CTGGGGCACC GTGACCACAG CGATTCCTCG 
TACCGGGCCC TGCCCGAGGA CACACTCACT TTTGAGGAAG CCGTCGCCAC CAACTCGGGC 
CGCTCATCGA GGACTTCCTA CGTGTCCTCT CTAACATCCC AGTCCCACCA TCTCCGTCGT 
GTGCCCAATG GTTACCACTG CAGTCTGGGG CTCAGCTCCG GCCCCGGGAC TGGCGGTGGC 
AGGGCCCGGC AGAGTTATTA TCACCAAACC GACGAAGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951861.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807721.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X13, mRNA.

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CloneID	OSa224811
Clone ID Related Accession (Same CDS sequence)	XM_031951861.1
Accession Version	XM_031951861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CAGGAAAAGG GAAAGACATC 
AGCACAATCA AGTCACTGCG AGTACTCCGA GTCCTGAGAC CTCTGAAGAC CATCAAACGT 
TTGCCAAAAC TAAAGGCTGT GTTTGACTGT GTGGTAAACT CCCTGAAGAA TGTCCTCAAT 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCCG TCATTGCTGT GCAGCTCTTC 
AAAGGAAAAT TCTTCTATTG CACAGATGAA TCCAAAGAGC TTCAGAGGGA TTGCAGGGGT 
CAGTATTTGG ATTATGAAAA GGACGAAGTG GAGGCTCAAC CCAGACAATG GAAGAAATAT 
GACTTTCACT ATGACAATGT GCTTTGGGCT TTGCTGACTC TTTTCACAGT GTCTACAGGA 
GAAGGGTGGC CAACGGTACT GAAGCATTCG GTGGATGCAA CATATGAAGA ACAGGGTCCA 
AGTCCTGGCT TCCGGATGGA ACTGTCAATC TTCTACGTGG TCTACTTTGT TGTGTTTCCC 
TTTTTCTTCG TCAATATCTT TGTGGCATTG ATCATCATCA CCTTCCAGGA GCAGGGAGAC 
AAAGTGATGT CAGAATGCAG CTTGGAGAAG AATGAGAGGG CTTGCATAGA CTTTGCCATC 
AGTGCCAAGC CCCTGACCCG TTACATGCCT CAGAACAAGC AGTCCTTCCA GTACAAGACA 
TGGACCTTTG TGGTGTCTCC ACCCTTTGAG TATTTCATCA TGGCCATGAT CGCCCTCAAC 
ACGGCCGTCT TGATGATGAA GTTCTATGGT GCCCCCTATG AGTATGAAAT GATGCTGAAG 
GGCTTGAATA TTGTATTCAC TTCAATGTTC TCTATGGAGT GTGTGCTGAA GATCATCGCC 
TTTGGGGTGC TGAATTATTT CAGAGATGCC TGGAATGTTT TTGATTTTGT AACAGTATTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGGAAA CGAACAACTT CATCAATCTC 
AGCTTCTTGC GGCTCTTTCG GGCAGCCCGA CTCATTAAAC TGCTTCGCCA GGGATATACT 
ATCCGAATTC TCCTGTGGAC CTTTGTTCAG TCCTTCAAGG CTCTGCCGTA TGTCTGCTTA 
CTCATCGCCA TGCTCTTCTT CATCTACGCC ATCATTGGCA TGCAGGTCTT TGGAAACATT 
GCTCTTGATG ATGAAACGAA TATCAACCGG CACAACAATT TCCGGACATT CCTGCAAGCC 
CTGATGCTGC TGTTCAGGAG TGCTACAGGG GAAGCCTGGC ATGAGATCAT GTTGTCATGT 
CTTAGTGACC GTCCCTGTGA TCCTGAAACC AAACTAAAAG ATGAATGTGG GAGTGACTTT 
GCTTACTTTT ACTTCGTCTC CTTCATTTTC CTCTGCTCCT TTCTGATGCT GAATCTCTTT 
GTGGCAGTGA TTATGGATAA TTTTGAGTAC CTGACTCGAG ACTCTTCCAT CCTAGGACCT 
CATCACCTGG ACGAATTTAT TCGGGTCTGG GCTGAATATG ACCCAGCTGC ATGTGGGCGC 
ATCAGTTACA GTGACATGTT TGAGATGCTG AAACACATGT CCCCACCCCT CGGCCTGGGA 
AAGAAATGCC CTGCTCGAGT TGCCTATAAG CGCCTGGTGC GAATGAACAT GCCCATCTCC 
AATGAGGACA TGACTGTGCA CTTCACTTCC ACCTTGATGG CCCTCATCCG GACGGCTCTG 
GACATCAAGC TTGCACCAGC GGGGATGAAA CAGCATCAGT GTGATGCAGA ACTGAGGAAA 
GAGATTGCAT CTGTTTGGGC TAATCTGCCC CAGAAGACAC TGGACTTACT GGTGCCTCCA 
CATAAACCTG ATGAGATGAC TGTAGGAAAA GTCTACGCAG CTCTGATGAT TTTTGACTTC 
TACAAGCAGA ACAAAAACAC CCGAGATCAA GGTCACCAGC CTCCGACAGG TCCTGTGTCT 
CTGTTCCATA CCCAAGGCCA ACCAGCTGTG CTCCGAGGTG CTAGAGTGTT CTTGAAACAG 
AAAAGTTCAA CCTCCCTAAG CAATGGTGGA GCCTTACCAC ACCCAGGGAG TGGTATCAAG 
GAGTCTGTCT CATGGGGCAC CCAGCGCACC CAGGATATCT GCTATGAGAC CAGGATGCCC 
CTGGAACGTG GTCATTCTGC AGAAATTCCT GTGGGACAAC CTAGCAAACA AGCTGTGGAG 
ATGCAAGAAA TGGCTCAGGA TGCCACCAGT GGAGAACCCC AGCCAGGGCT GGAAAGTCAG 
GGCCGAGCGG CCTCCATGCC CCGCCTGGCT GCAGAGATGC AGCCCATTCC AGACACAAGC 
CCCATGAAGC GTTCCATCTC CACCTTGGCC CCACAGCGCC CACATGGCTC ACACCTTTAT 
GAATATGGTC TGGAACGCGT ATCCTCAGGC CAGCCTCAGC ATCATCACCA TCATCGCTGT 
CACCGGCGCA GGGATAAGAA GCAGAAGTCT TTGGACAAAT GCACTAGTCA GTCAGCAGAT 
GGAGATGGGG CACCAAACCA TAGTGTGGGG ATGGCAGCTC CATCAGGGGA AGGTCCAGCC 
CCATGTAAGC ATGAGAGGAA GCAAGAAAGA GGGCGATCCC AGGAGCGGAA GCAGCTCTCT 
TCCTCCTCTT CAGAGAAGCA GCGTTTCTAT TCTTGTGACC GATTTGGGAG CCGGGACCCA 
TCACAGCCCA AATCCTCCAA TGGAAGTCGT CCTACATCAC CTCCTGGGCA GGAGCCAGGT 
CCCCACAGAC AGGGTAGTGG TTCAGTTAAT GGGAGCCCCT TGCTGTCAAC ATCTGGTGCT 
AGTACACCGG GCCGTGGTGG GCGGAGGCAG CTCCCCCAGA CACCTCTGAC CCCCCGTCCC 
AGCATCACAT ACAAGACTGC GAACTCCTCG CCCGTCCACT TCACCGGTTT CCAGACCAGC 
CTGCCCACCT TCTCCCCGGG ACGGCTCAGT CGAGGCCTTT CTGAGCACAA CACCCTGCTC 
CAGAGGGATT CTCTGAGCCA GCCGCCAACC CCAGTGTCTC GGATTGGCTC GGACCCATAC 
CTGGGGCACC GTGACCACAG CGATTCCTCG TACCGGGCCC TGCCCGAGGA CACACTCACT 
TTTGAGGAAG CCGTCGCCAC CAACTCGGGC CGCTCATCGA GGACTTCCTA CGTGTCCTCT 
CTAACATCCC AGTCCCACCA TCTCCGTCGT GTGCCCAATG GTTACCACTG CAGTCTGGGG 
CTCAGCTCCG GCCCCGGGAC TGGCGGTGGC AGGGCCCGGC AGAGTTATTA TCACCAAACC 
GACGAAGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807721.1
CDS	1..7041
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951861.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951861.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CAGGAAAAGG GAAAGACATC 
AGCACAATCA AGTCACTGCG AGTACTCCGA GTCCTGAGAC CTCTGAAGAC CATCAAACGT 
TTGCCAAAAC TAAAGGCTGT GTTTGACTGT GTGGTAAACT CCCTGAAGAA TGTCCTCAAT 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCCG TCATTGCTGT GCAGCTCTTC 
AAAGGAAAAT TCTTCTATTG CACAGATGAA TCCAAAGAGC TTCAGAGGGA TTGCAGGGGT 
CAGTATTTGG ATTATGAAAA GGACGAAGTG GAGGCTCAAC CCAGACAATG GAAGAAATAT 
GACTTTCACT ATGACAATGT GCTTTGGGCT TTGCTGACTC TTTTCACAGT GTCTACAGGA 
GAAGGGTGGC CAACGGTACT GAAGCATTCG GTGGATGCAA CATATGAAGA ACAGGGTCCA 
AGTCCTGGCT TCCGGATGGA ACTGTCAATC TTCTACGTGG TCTACTTTGT TGTGTTTCCC 
TTTTTCTTCG TCAATATCTT TGTGGCATTG ATCATCATCA CCTTCCAGGA GCAGGGAGAC 
AAAGTGATGT CAGAATGCAG CTTGGAGAAG AATGAGAGGG CTTGCATAGA CTTTGCCATC 
AGTGCCAAGC CCCTGACCCG TTACATGCCT CAGAACAAGC AGTCCTTCCA GTACAAGACA 
TGGACCTTTG TGGTGTCTCC ACCCTTTGAG TATTTCATCA TGGCCATGAT CGCCCTCAAC 
ACGGCCGTCT TGATGATGAA GTTCTATGGT GCCCCCTATG AGTATGAAAT GATGCTGAAG 
GGCTTGAATA TTGTATTCAC TTCAATGTTC TCTATGGAGT GTGTGCTGAA GATCATCGCC 
TTTGGGGTGC TGAATTATTT CAGAGATGCC TGGAATGTTT TTGATTTTGT AACAGTATTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGGAAA CGAACAACTT CATCAATCTC 
AGCTTCTTGC GGCTCTTTCG GGCAGCCCGA CTCATTAAAC TGCTTCGCCA GGGATATACT 
ATCCGAATTC TCCTGTGGAC CTTTGTTCAG TCCTTCAAGG CTCTGCCGTA TGTCTGCTTA 
CTCATCGCCA TGCTCTTCTT CATCTACGCC ATCATTGGCA TGCAGGTCTT TGGAAACATT 
GCTCTTGATG ATGAAACGAA TATCAACCGG CACAACAATT TCCGGACATT CCTGCAAGCC 
CTGATGCTGC TGTTCAGGAG TGCTACAGGG GAAGCCTGGC ATGAGATCAT GTTGTCATGT 
CTTAGTGACC GTCCCTGTGA TCCTGAAACC AAACTAAAAG ATGAATGTGG GAGTGACTTT 
GCTTACTTTT ACTTCGTCTC CTTCATTTTC CTCTGCTCCT TTCTGATGCT GAATCTCTTT 
GTGGCAGTGA TTATGGATAA TTTTGAGTAC CTGACTCGAG ACTCTTCCAT CCTAGGACCT 
CATCACCTGG ACGAATTTAT TCGGGTCTGG GCTGAATATG ACCCAGCTGC ATGTGGGCGC 
ATCAGTTACA GTGACATGTT TGAGATGCTG AAACACATGT CCCCACCCCT CGGCCTGGGA 
AAGAAATGCC CTGCTCGAGT TGCCTATAAG CGCCTGGTGC GAATGAACAT GCCCATCTCC 
AATGAGGACA TGACTGTGCA CTTCACTTCC ACCTTGATGG CCCTCATCCG GACGGCTCTG 
GACATCAAGC TTGCACCAGC GGGGATGAAA CAGCATCAGT GTGATGCAGA ACTGAGGAAA 
GAGATTGCAT CTGTTTGGGC TAATCTGCCC CAGAAGACAC TGGACTTACT GGTGCCTCCA 
CATAAACCTG ATGAGATGAC TGTAGGAAAA GTCTACGCAG CTCTGATGAT TTTTGACTTC 
TACAAGCAGA ACAAAAACAC CCGAGATCAA GGTCACCAGC CTCCGACAGG TCCTGTGTCT 
CTGTTCCATA CCCAAGGCCA ACCAGCTGTG CTCCGAGGTG CTAGAGTGTT CTTGAAACAG 
AAAAGTTCAA CCTCCCTAAG CAATGGTGGA GCCTTACCAC ACCCAGGGAG TGGTATCAAG 
GAGTCTGTCT CATGGGGCAC CCAGCGCACC CAGGATATCT GCTATGAGAC CAGGATGCCC 
CTGGAACGTG GTCATTCTGC AGAAATTCCT GTGGGACAAC CTAGCAAACA AGCTGTGGAG 
ATGCAAGAAA TGGCTCAGGA TGCCACCAGT GGAGAACCCC AGCCAGGGCT GGAAAGTCAG 
GGCCGAGCGG CCTCCATGCC CCGCCTGGCT GCAGAGATGC AGCCCATTCC AGACACAAGC 
CCCATGAAGC GTTCCATCTC CACCTTGGCC CCACAGCGCC CACATGGCTC ACACCTTTAT 
GAATATGGTC TGGAACGCGT ATCCTCAGGC CAGCCTCAGC ATCATCACCA TCATCGCTGT 
CACCGGCGCA GGGATAAGAA GCAGAAGTCT TTGGACAAAT GCACTAGTCA GTCAGCAGAT 
GGAGATGGGG CACCAAACCA TAGTGTGGGG ATGGCAGCTC CATCAGGGGA AGGTCCAGCC 
CCATGTAAGC ATGAGAGGAA GCAAGAAAGA GGGCGATCCC AGGAGCGGAA GCAGCTCTCT 
TCCTCCTCTT CAGAGAAGCA GCGTTTCTAT TCTTGTGACC GATTTGGGAG CCGGGACCCA 
TCACAGCCCA AATCCTCCAA TGGAAGTCGT CCTACATCAC CTCCTGGGCA GGAGCCAGGT 
CCCCACAGAC AGGGTAGTGG TTCAGTTAAT GGGAGCCCCT TGCTGTCAAC ATCTGGTGCT 
AGTACACCGG GCCGTGGTGG GCGGAGGCAG CTCCCCCAGA CACCTCTGAC CCCCCGTCCC 
AGCATCACAT ACAAGACTGC GAACTCCTCG CCCGTCCACT TCACCGGTTT CCAGACCAGC 
CTGCCCACCT TCTCCCCGGG ACGGCTCAGT CGAGGCCTTT CTGAGCACAA CACCCTGCTC 
CAGAGGGATT CTCTGAGCCA GCCGCCAACC CCAGTGTCTC GGATTGGCTC GGACCCATAC 
CTGGGGCACC GTGACCACAG CGATTCCTCG TACCGGGCCC TGCCCGAGGA CACACTCACT 
TTTGAGGAAG CCGTCGCCAC CAACTCGGGC CGCTCATCGA GGACTTCCTA CGTGTCCTCT 
CTAACATCCC AGTCCCACCA TCTCCGTCGT GTGCCCAATG GTTACCACTG CAGTCTGGGG 
CTCAGCTCCG GCCCCGGGAC TGGCGGTGGC AGGGCCCGGC AGAGTTATTA TCACCAAACC 
GACGAAGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951863.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807723.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X14, mRNA.

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CloneID	OSa224812
Clone ID Related Accession (Same CDS sequence)	XM_031951863.1
Accession Version	XM_031951863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CAGGAAAAGG GAAAGACATC 
AGCACAATCA AGTCACTGCG AGTACTCCGA GTCCTGAGAC CTCTGAAGAC CATCAAACGT 
TTGCCAAAAC TAAAGGCTGT GTTTGACTGT GTGGTAAACT CCCTGAAGAA TGTCCTCAAT 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCCG TCATTGCTGT GCAGCTCTTC 
AAAGGAAAAT TCTTCTATTG CACAGATGAA TCCAAAGAGC TTCAGAGGGA TTGCAGGGGT 
CAGTATTTGG ATTATGAAAA GGACGAAGTG GAGGCTCAAC CCAGACAATG GAAGAAATAT 
GACTTTCACT ATGACAATGT GCTTTGGGCT TTGCTGACTC TTTTCACAGT GTCTACAGGA 
GAAGGGTGGC CAACGGTACT GAAGCATTCG GTGGATGCAA CATATGAAGA ACAGGGTCCA 
AGTCCTGGCT TCCGGATGGA ACTGTCAATC TTCTACGTGG TCTACTTTGT TGTGTTTCCC 
TTTTTCTTCG TCAATATCTT TGTGGCATTG ATCATCATCA CCTTCCAGGA GCAGGGAGAC 
AAAGTGATGT CAGAATGCAG CTTGGAGAAG AATGAGAGGG CTTGCATAGA CTTTGCCATC 
AGTGCCAAGC CCCTGACCCG TTACATGCCT CAGAACAAGC AGTCCTTCCA GTACAAGACA 
TGGACCTTTG TGGTGTCTCC ACCCTTTGAG TATTTCATCA TGGCCATGAT CGCCCTCAAC 
ACGGCCGTCT TGATGATGAA GTTCTATGGT GCCCCCTATG AGTATGAAAT GATGCTGAAG 
GGCTTGAATA TTGTATTCAC TTCAATGTTC TCTATGGAGT GTGTGCTGAA GATCATCGCC 
TTTGGGGTGC TGAATTATTT CAGAGATGCC TGGAATGTTT TTGATTTTGT AACAGTATTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGAACA ACTTCATCAA TCTCAGCTTC 
TTGCGGCTCT TTCGGGCAGC CCGACTCATT AAACTGCTTC GCCAGGGATA TACTATCCGA 
ATTCTCCTGT GGACCTTTGT TCAGTCCTTC AAGGCTCTGC CGTATGTCTG CTTACTCATC 
GCCATGCTCT TCTTCATCTA CGCCATCATT GGCATGCAGG TCTTTGGAAA CATTGCTCTT 
GATGATGAAA CGAATATCAA CCGGCACAAC AATTTCCGGA CATTCCTGCA AGCCCTGATG 
CTGCTGTTCA GGAGTGCTAC AGGGGAAGCC TGGCATGAGA TCATGTTGTC ATGTCTTAGT 
GACCGTCCCT GTGATCCTGA AACCAAACTA AAAGATGAAT GTGGGAGTGA CTTTGCTTAC 
TTTTACTTCG TCTCCTTCAT TTTCCTCTGC TCCTTTCTGA TGCTGAATCT CTTTGTGGCA 
GTGATTATGG ATAATTTTGA GTACCTGACT CGAGACTCTT CCATCCTAGG ACCTCATCAC 
CTGGACGAAT TTATTCGGGT CTGGGCTGAA TATGACCCAG CTGCATGTGG GCGCATCAGT 
TACAGTGACA TGTTTGAGAT GCTGAAACAC ATGTCCCCAC CCCTCGGCCT GGGAAAGAAA 
TGCCCTGCTC GAGTTGCCTA TAAGCGCCTG GTGCGAATGA ACATGCCCAT CTCCAATGAG 
GACATGACTG TGCACTTCAC TTCCACCTTG ATGGCCCTCA TCCGGACGGC TCTGGACATC 
AAGCTTGCAC CAGCGGGGAT GAAACAGCAT CAGTGTGATG CAGAACTGAG GAAAGAGATT 
GCATCTGTTT GGGCTAATCT GCCCCAGAAG ACACTGGACT TACTGGTGCC TCCACATAAA 
CCTGATGAGA TGACTGTAGG AAAAGTCTAC GCAGCTCTGA TGATTTTTGA CTTCTACAAG 
CAGAACAAAA ACACCCGAGA TCAAGGTCAC CAGCCTCCGA CAGGTCCTGT GTCTCTGTTC 
CATACCCAAG GCCAACCAGC TGTGCTCCGA GGTGCTAGAG TGTTCTTGAA ACAGAAAAGT 
TCAACCTCCC TAAGCAATGG TGGAGCCTTA CCACACCCAG GGAGTGGTAT CAAGGAGTCT 
GTCTCATGGG GCACCCAGCG CACCCAGGAT ATCTGCTATG AGACCAGGAT GCCCCTGGAA 
CGTGGTCATT CTGCAGAAAT TCCTGTGGGA CAACCTAGCA AACAAGCTGT GGAGATGCAA 
GAAATGGCTC AGGATGCCAC CAGTGGAGAA CCCCAGCCAG GGCTGGAAAG TCAGGGCCGA 
GCGGCCTCCA TGCCCCGCCT GGCTGCAGAG ATGCAGCCCA TTCCAGACAC AAGCCCCATG 
AAGCGTTCCA TCTCCACCTT GGCCCCACAG CGCCCACATG GCTCACACCT TTATGAATAT 
GGTCTGGAAC GCGTATCCTC AGGCCAGCCT CAGCATCATC ACCATCATCG CTGTCACCGG 
CGCAGGGATA AGAAGCAGAA GTCTTTGGAC AAATGCACTA GTCAGTCAGC AGATGGAGAT 
GGGGCACCAA ACCATAGTGT GGGGATGGCA GCTCCATCAG GGGAAGGTCC AGCCCCATGT 
AAGCATGAGA GGAAGCAAGA AAGAGGGCGA TCCCAGGAGC GGAAGCAGCT CTCTTCCTCC 
TCTTCAGAGA AGCAGCGTTT CTATTCTTGT GACCGATTTG GGAGCCGGGA CCCATCACAG 
CCCAAATCCT CCAATGGAAG TCGTCCTACA TCACCTCCTG GGCAGGAGCC AGGTCCCCAC 
AGACAGGGTA GTGGTTCAGT TAATGGGAGC CCCTTGCTGT CAACATCTGG TGCTAGTACA 
CCGGGCCGTG GTGGGCGGAG GCAGCTCCCC CAGACACCTC TGACCCCCCG TCCCAGCATC 
ACATACAAGA CTGCGAACTC CTCGCCCGTC CACTTCACCG GTTTCCAGAC CAGCCTGCCC 
ACCTTCTCCC CGGGACGGCT CAGTCGAGGC CTTTCTGAGC ACAACACCCT GCTCCAGAGG 
GATTCTCTGA GCCAGCCGCC AACCCCAGTG TCTCGGATTG GCTCGGACCC ATACCTGGGG 
CACCGTGACC ACAGCGATTC CTCGTACCGG GCCCTGCCCG AGGACACACT CACTTTTGAG 
GAAGCCGTCG CCACCAACTC GGGCCGCTCA TCGAGGACTT CCTACGTGTC CTCTCTAACA 
TCCCAGTCCC ACCATCTCCG TCGTGTGCCC AATGGTTACC ACTGCAGTCT GGGGCTCAGC 
TCCGGCCCCG GGACTGGCGG TGGCAGGGCC CGGCAGAGTT ATTATCACCA AACCGACGAA 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807723.1
CDS	1..7035
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951863.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951863.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CAGGAAAAGG GAAAGACATC 
AGCACAATCA AGTCACTGCG AGTACTCCGA GTCCTGAGAC CTCTGAAGAC CATCAAACGT 
TTGCCAAAAC TAAAGGCTGT GTTTGACTGT GTGGTAAACT CCCTGAAGAA TGTCCTCAAT 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCCG TCATTGCTGT GCAGCTCTTC 
AAAGGAAAAT TCTTCTATTG CACAGATGAA TCCAAAGAGC TTCAGAGGGA TTGCAGGGGT 
CAGTATTTGG ATTATGAAAA GGACGAAGTG GAGGCTCAAC CCAGACAATG GAAGAAATAT 
GACTTTCACT ATGACAATGT GCTTTGGGCT TTGCTGACTC TTTTCACAGT GTCTACAGGA 
GAAGGGTGGC CAACGGTACT GAAGCATTCG GTGGATGCAA CATATGAAGA ACAGGGTCCA 
AGTCCTGGCT TCCGGATGGA ACTGTCAATC TTCTACGTGG TCTACTTTGT TGTGTTTCCC 
TTTTTCTTCG TCAATATCTT TGTGGCATTG ATCATCATCA CCTTCCAGGA GCAGGGAGAC 
AAAGTGATGT CAGAATGCAG CTTGGAGAAG AATGAGAGGG CTTGCATAGA CTTTGCCATC 
AGTGCCAAGC CCCTGACCCG TTACATGCCT CAGAACAAGC AGTCCTTCCA GTACAAGACA 
TGGACCTTTG TGGTGTCTCC ACCCTTTGAG TATTTCATCA TGGCCATGAT CGCCCTCAAC 
ACGGCCGTCT TGATGATGAA GTTCTATGGT GCCCCCTATG AGTATGAAAT GATGCTGAAG 
GGCTTGAATA TTGTATTCAC TTCAATGTTC TCTATGGAGT GTGTGCTGAA GATCATCGCC 
TTTGGGGTGC TGAATTATTT CAGAGATGCC TGGAATGTTT TTGATTTTGT AACAGTATTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGAACA ACTTCATCAA TCTCAGCTTC 
TTGCGGCTCT TTCGGGCAGC CCGACTCATT AAACTGCTTC GCCAGGGATA TACTATCCGA 
ATTCTCCTGT GGACCTTTGT TCAGTCCTTC AAGGCTCTGC CGTATGTCTG CTTACTCATC 
GCCATGCTCT TCTTCATCTA CGCCATCATT GGCATGCAGG TCTTTGGAAA CATTGCTCTT 
GATGATGAAA CGAATATCAA CCGGCACAAC AATTTCCGGA CATTCCTGCA AGCCCTGATG 
CTGCTGTTCA GGAGTGCTAC AGGGGAAGCC TGGCATGAGA TCATGTTGTC ATGTCTTAGT 
GACCGTCCCT GTGATCCTGA AACCAAACTA AAAGATGAAT GTGGGAGTGA CTTTGCTTAC 
TTTTACTTCG TCTCCTTCAT TTTCCTCTGC TCCTTTCTGA TGCTGAATCT CTTTGTGGCA 
GTGATTATGG ATAATTTTGA GTACCTGACT CGAGACTCTT CCATCCTAGG ACCTCATCAC 
CTGGACGAAT TTATTCGGGT CTGGGCTGAA TATGACCCAG CTGCATGTGG GCGCATCAGT 
TACAGTGACA TGTTTGAGAT GCTGAAACAC ATGTCCCCAC CCCTCGGCCT GGGAAAGAAA 
TGCCCTGCTC GAGTTGCCTA TAAGCGCCTG GTGCGAATGA ACATGCCCAT CTCCAATGAG 
GACATGACTG TGCACTTCAC TTCCACCTTG ATGGCCCTCA TCCGGACGGC TCTGGACATC 
AAGCTTGCAC CAGCGGGGAT GAAACAGCAT CAGTGTGATG CAGAACTGAG GAAAGAGATT 
GCATCTGTTT GGGCTAATCT GCCCCAGAAG ACACTGGACT TACTGGTGCC TCCACATAAA 
CCTGATGAGA TGACTGTAGG AAAAGTCTAC GCAGCTCTGA TGATTTTTGA CTTCTACAAG 
CAGAACAAAA ACACCCGAGA TCAAGGTCAC CAGCCTCCGA CAGGTCCTGT GTCTCTGTTC 
CATACCCAAG GCCAACCAGC TGTGCTCCGA GGTGCTAGAG TGTTCTTGAA ACAGAAAAGT 
TCAACCTCCC TAAGCAATGG TGGAGCCTTA CCACACCCAG GGAGTGGTAT CAAGGAGTCT 
GTCTCATGGG GCACCCAGCG CACCCAGGAT ATCTGCTATG AGACCAGGAT GCCCCTGGAA 
CGTGGTCATT CTGCAGAAAT TCCTGTGGGA CAACCTAGCA AACAAGCTGT GGAGATGCAA 
GAAATGGCTC AGGATGCCAC CAGTGGAGAA CCCCAGCCAG GGCTGGAAAG TCAGGGCCGA 
GCGGCCTCCA TGCCCCGCCT GGCTGCAGAG ATGCAGCCCA TTCCAGACAC AAGCCCCATG 
AAGCGTTCCA TCTCCACCTT GGCCCCACAG CGCCCACATG GCTCACACCT TTATGAATAT 
GGTCTGGAAC GCGTATCCTC AGGCCAGCCT CAGCATCATC ACCATCATCG CTGTCACCGG 
CGCAGGGATA AGAAGCAGAA GTCTTTGGAC AAATGCACTA GTCAGTCAGC AGATGGAGAT 
GGGGCACCAA ACCATAGTGT GGGGATGGCA GCTCCATCAG GGGAAGGTCC AGCCCCATGT 
AAGCATGAGA GGAAGCAAGA AAGAGGGCGA TCCCAGGAGC GGAAGCAGCT CTCTTCCTCC 
TCTTCAGAGA AGCAGCGTTT CTATTCTTGT GACCGATTTG GGAGCCGGGA CCCATCACAG 
CCCAAATCCT CCAATGGAAG TCGTCCTACA TCACCTCCTG GGCAGGAGCC AGGTCCCCAC 
AGACAGGGTA GTGGTTCAGT TAATGGGAGC CCCTTGCTGT CAACATCTGG TGCTAGTACA 
CCGGGCCGTG GTGGGCGGAG GCAGCTCCCC CAGACACCTC TGACCCCCCG TCCCAGCATC 
ACATACAAGA CTGCGAACTC CTCGCCCGTC CACTTCACCG GTTTCCAGAC CAGCCTGCCC 
ACCTTCTCCC CGGGACGGCT CAGTCGAGGC CTTTCTGAGC ACAACACCCT GCTCCAGAGG 
GATTCTCTGA GCCAGCCGCC AACCCCAGTG TCTCGGATTG GCTCGGACCC ATACCTGGGG 
CACCGTGACC ACAGCGATTC CTCGTACCGG GCCCTGCCCG AGGACACACT CACTTTTGAG 
GAAGCCGTCG CCACCAACTC GGGCCGCTCA TCGAGGACTT CCTACGTGTC CTCTCTAACA 
TCCCAGTCCC ACCATCTCCG TCGTGTGCCC AATGGTTACC ACTGCAGTCT GGGGCTCAGC 
TCCGGCCCCG GGACTGGCGG TGGCAGGGCC CGGCAGAGTT ATTATCACCA AACCGACGAA 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951864.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807724.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X15, mRNA.

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CloneID	OSa224813
Clone ID Related Accession (Same CDS sequence)	XM_031951864.1
Accession Version	XM_031951864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCAGGAAA AGGGAAAGAC 
ATCAGCACAA TCAAGTCACT GCGAGTACTC CGAGTCCTGA GACCTCTGAA GACCATCAAA 
CGTTTGCCAA AACTAAAGGC TGTGTTTGAC TGTGTGGTAA ACTCCCTGAA GAATGTCCTC 
AATATCCTCA TTGTCTACAT GCTCTTCATG TTCATCTTTG CCGTCATTGC TGTGCAGCTC 
TTCAAAGGAA AATTCTTCTA TTGCACAGAT GAATCCAAAG AGCTTCAGAG GGATTGCAGG 
GGTCAGTATT TGGATTATGA AAAGGACGAA GTGGAGGCTC AACCCAGACA ATGGAAGAAA 
TATGACTTTC ACTATGACAA TGTGCTTTGG GCTTTGCTGA CTCTTTTCAC AGTGTCTACA 
GGAGAAGGGT GGCCAACGGT ACTGAAGCAT TCGGTGGATG CAACATATGA AGAACAGGGT 
CCAAGTCCTG GCTTCCGGAT GGAACTGTCA ATCTTCTACG TGGTCTACTT TGTTGTGTTT 
CCCTTTTTCT TCGTCAATAT CTTTGTGGCA TTGATCATCA TCACCTTCCA GGAGCAGGGA 
GACAAAGTGA TGTCAGAATG CAGCTTGGAG AAGAATGAGA GGGCTTGCAT AGACTTTGCC 
ATCAGTGCCA AGCCCCTGAC CCGTTACATG CCTCAGAACA AGCAGTCCTT CCAGTACAAG 
ACATGGACCT TTGTGGTGTC TCCACCCTTT GAGTATTTCA TCATGGCCAT GATCGCCCTC 
AACACGGCCG TCTTGATGAT GAAGTTCTAT GGTGCCCCCT ATGAGTATGA AATGATGCTG 
AAGGGCTTGA ATATTGTATT CACTTCAATG TTCTCTATGG AGTGTGTGCT GAAGATCATC 
GCCTTTGGGG TGCTGAATTA TTTCAGAGAT GCCTGGAATG TTTTTGATTT TGTAACAGTA 
TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGA ACAACTTCAT CAATCTCAGC 
TTCTTGCGGC TCTTTCGGGC AGCCCGACTC ATTAAACTGC TTCGCCAGGG ATATACTATC 
CGAATTCTCC TGTGGACCTT TGTTCAGTCC TTCAAGGCTC TGCCGTATGT CTGCTTACTC 
ATCGCCATGC TCTTCTTCAT CTACGCCATC ATTGGCATGC AGGTCTTTGG AAACATTGCT 
CTTGATGATG AAACGAATAT CAACCGGCAC AACAATTTCC GGACATTCCT GCAAGCCCTG 
ATGCTGCTGT TCAGGAGTGC TACAGGGGAA GCCTGGCATG AGATCATGTT GTCATGTCTT 
AGTGACCGTC CCTGTGATCC TGAAACCAAA CTAAAAGATG AATGTGGGAG TGACTTTGCT 
TACTTTTACT TCGTCTCCTT CATTTTCCTC TGCTCCTTTC TGATGCTGAA TCTCTTTGTG 
GCAGTGATTA TGGATAATTT TGAGTACCTG ACTCGAGACT CTTCCATCCT AGGACCTCAT 
CACCTGGACG AATTTATTCG GGTCTGGGCT GAATATGACC CAGCTGCATG TGGGCGCATC 
AGTTACAGTG ACATGTTTGA GATGCTGAAA CACATGTCCC CACCCCTCGG CCTGGGAAAG 
AAATGCCCTG CTCGAGTTGC CTATAAGCGC CTGGTGCGAA TGAACATGCC CATCTCCAAT 
GAGGACATGA CTGTGCACTT CACTTCCACC TTGATGGCCC TCATCCGGAC GGCTCTGGAC 
ATCAAGCTTG CACCAGCGGG GATGAAACAG CATCAGTGTG ATGCAGAACT GAGGAAAGAG 
ATTGCATCTG TTTGGGCTAA TCTGCCCCAG AAGACACTGG ACTTACTGGT GCCTCCACAT 
AAACCTGATG AGATGACTGT AGGAAAAGTC TACGCAGCTC TGATGATTTT TGACTTCTAC 
AAGCAGAACA AAAACACCCG AGATCAAGGT CACCAGCCTC CGACAGGTCC TGTGTCTCTG 
TTCCATACCC AAGGCCAACC AGCTGTGCTC CGAGGTGCTA GAGTGTTCTT GAAACAGAAA 
AGTTCAACCT CCCTAAGCAA TGGTGGAGCC TTACCACACC CAGGGAGTGG TATCAAGGAG 
TCTGTCTCAT GGGGCACCCA GCGCACCCAG GATATCTGCT ATGAGACCAG GATGCCCCTG 
GAACGTGGTC ATTCTGCAGA AATTCCTGTG GGACAACCTA GCAAACAAGC TGTGGAGATG 
CAAGAAATGG CTCAGGATGC CACCAGTGGA GAACCCCAGC CAGGGCTGGA AAGTCAGGGC 
CGAGCGGCCT CCATGCCCCG CCTGGCTGCA GAGATGCAGC CCATTCCAGA CACAAGCCCC 
ATGAAGCGTT CCATCTCCAC CTTGGCCCCA CAGCGCCCAC ATGGCTCACA CCTTTATGAA 
TATGGTCTGG AACGCGTATC CTCAGGCCAG CCTCAGCATC ATCACCATCA TCGCTGTCAC 
CGGCGCAGGG ATAAGAAGCA GAAGTCTTTG GACAAATGCA CTAGTCAGTC AGCAGATGGA 
GATGGGGCAC CAAACCATAG TGTGGGGATG GCAGCTCCAT CAGGGGAAGG TCCAGCCCCA 
TGTAAGCATG AGAGGAAGCA AGAAAGAGGG CGATCCCAGG AGCGGAAGCA GCTCTCTTCC 
TCCTCTTCAG AGAAGCAGCG TTTCTATTCT TGTGACCGAT TTGGGAGCCG GGACCCATCA 
CAGCCCAAAT CCTCCAATGG AAGTCGTCCT ACATCACCTC CTGGGCAGGA GCCAGGTCCC 
CACAGACAGG GTAGTGGTTC AGTTAATGGG AGCCCCTTGC TGTCAACATC TGGTGCTAGT 
ACACCGGGCC GTGGTGGGCG GAGGCAGCTC CCCCAGACAC CTCTGACCCC CCGTCCCAGC 
ATCACATACA AGACTGCGAA CTCCTCGCCC GTCCACTTCA CCGGTTTCCA GACCAGCCTG 
CCCACCTTCT CCCCGGGACG GCTCAGTCGA GGCCTTTCTG AGCACAACAC CCTGCTCCAG 
AGGGATTCTC TGAGCCAGCC GCCAACCCCA GTGTCTCGGA TTGGCTCGGA CCCATACCTG 
GGGCACCGTG ACCACAGCGA TTCCTCGTAC CGGGCCCTGC CCGAGGACAC ACTCACTTTT 
GAGGAAGCCG TCGCCACCAA CTCGGGCCGC TCATCGAGGA CTTCCTACGT GTCCTCTCTA 
ACATCCCAGT CCCACCATCT CCGTCGTGTG CCCAATGGTT ACCACTGCAG TCTGGGGCTC 
AGCTCCGGCC CCGGGACTGG CGGTGGCAGG GCCCGGCAGA GTTATTATCA CCAAACCGAC 
GAAGATGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807724.1
CDS	1..7038
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951864.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951864.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCAGGAAA AGGGAAAGAC 
ATCAGCACAA TCAAGTCACT GCGAGTACTC CGAGTCCTGA GACCTCTGAA GACCATCAAA 
CGTTTGCCAA AACTAAAGGC TGTGTTTGAC TGTGTGGTAA ACTCCCTGAA GAATGTCCTC 
AATATCCTCA TTGTCTACAT GCTCTTCATG TTCATCTTTG CCGTCATTGC TGTGCAGCTC 
TTCAAAGGAA AATTCTTCTA TTGCACAGAT GAATCCAAAG AGCTTCAGAG GGATTGCAGG 
GGTCAGTATT TGGATTATGA AAAGGACGAA GTGGAGGCTC AACCCAGACA ATGGAAGAAA 
TATGACTTTC ACTATGACAA TGTGCTTTGG GCTTTGCTGA CTCTTTTCAC AGTGTCTACA 
GGAGAAGGGT GGCCAACGGT ACTGAAGCAT TCGGTGGATG CAACATATGA AGAACAGGGT 
CCAAGTCCTG GCTTCCGGAT GGAACTGTCA ATCTTCTACG TGGTCTACTT TGTTGTGTTT 
CCCTTTTTCT TCGTCAATAT CTTTGTGGCA TTGATCATCA TCACCTTCCA GGAGCAGGGA 
GACAAAGTGA TGTCAGAATG CAGCTTGGAG AAGAATGAGA GGGCTTGCAT AGACTTTGCC 
ATCAGTGCCA AGCCCCTGAC CCGTTACATG CCTCAGAACA AGCAGTCCTT CCAGTACAAG 
ACATGGACCT TTGTGGTGTC TCCACCCTTT GAGTATTTCA TCATGGCCAT GATCGCCCTC 
AACACGGCCG TCTTGATGAT GAAGTTCTAT GGTGCCCCCT ATGAGTATGA AATGATGCTG 
AAGGGCTTGA ATATTGTATT CACTTCAATG TTCTCTATGG AGTGTGTGCT GAAGATCATC 
GCCTTTGGGG TGCTGAATTA TTTCAGAGAT GCCTGGAATG TTTTTGATTT TGTAACAGTA 
TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGA ACAACTTCAT CAATCTCAGC 
TTCTTGCGGC TCTTTCGGGC AGCCCGACTC ATTAAACTGC TTCGCCAGGG ATATACTATC 
CGAATTCTCC TGTGGACCTT TGTTCAGTCC TTCAAGGCTC TGCCGTATGT CTGCTTACTC 
ATCGCCATGC TCTTCTTCAT CTACGCCATC ATTGGCATGC AGGTCTTTGG AAACATTGCT 
CTTGATGATG AAACGAATAT CAACCGGCAC AACAATTTCC GGACATTCCT GCAAGCCCTG 
ATGCTGCTGT TCAGGAGTGC TACAGGGGAA GCCTGGCATG AGATCATGTT GTCATGTCTT 
AGTGACCGTC CCTGTGATCC TGAAACCAAA CTAAAAGATG AATGTGGGAG TGACTTTGCT 
TACTTTTACT TCGTCTCCTT CATTTTCCTC TGCTCCTTTC TGATGCTGAA TCTCTTTGTG 
GCAGTGATTA TGGATAATTT TGAGTACCTG ACTCGAGACT CTTCCATCCT AGGACCTCAT 
CACCTGGACG AATTTATTCG GGTCTGGGCT GAATATGACC CAGCTGCATG TGGGCGCATC 
AGTTACAGTG ACATGTTTGA GATGCTGAAA CACATGTCCC CACCCCTCGG CCTGGGAAAG 
AAATGCCCTG CTCGAGTTGC CTATAAGCGC CTGGTGCGAA TGAACATGCC CATCTCCAAT 
GAGGACATGA CTGTGCACTT CACTTCCACC TTGATGGCCC TCATCCGGAC GGCTCTGGAC 
ATCAAGCTTG CACCAGCGGG GATGAAACAG CATCAGTGTG ATGCAGAACT GAGGAAAGAG 
ATTGCATCTG TTTGGGCTAA TCTGCCCCAG AAGACACTGG ACTTACTGGT GCCTCCACAT 
AAACCTGATG AGATGACTGT AGGAAAAGTC TACGCAGCTC TGATGATTTT TGACTTCTAC 
AAGCAGAACA AAAACACCCG AGATCAAGGT CACCAGCCTC CGACAGGTCC TGTGTCTCTG 
TTCCATACCC AAGGCCAACC AGCTGTGCTC CGAGGTGCTA GAGTGTTCTT GAAACAGAAA 
AGTTCAACCT CCCTAAGCAA TGGTGGAGCC TTACCACACC CAGGGAGTGG TATCAAGGAG 
TCTGTCTCAT GGGGCACCCA GCGCACCCAG GATATCTGCT ATGAGACCAG GATGCCCCTG 
GAACGTGGTC ATTCTGCAGA AATTCCTGTG GGACAACCTA GCAAACAAGC TGTGGAGATG 
CAAGAAATGG CTCAGGATGC CACCAGTGGA GAACCCCAGC CAGGGCTGGA AAGTCAGGGC 
CGAGCGGCCT CCATGCCCCG CCTGGCTGCA GAGATGCAGC CCATTCCAGA CACAAGCCCC 
ATGAAGCGTT CCATCTCCAC CTTGGCCCCA CAGCGCCCAC ATGGCTCACA CCTTTATGAA 
TATGGTCTGG AACGCGTATC CTCAGGCCAG CCTCAGCATC ATCACCATCA TCGCTGTCAC 
CGGCGCAGGG ATAAGAAGCA GAAGTCTTTG GACAAATGCA CTAGTCAGTC AGCAGATGGA 
GATGGGGCAC CAAACCATAG TGTGGGGATG GCAGCTCCAT CAGGGGAAGG TCCAGCCCCA 
TGTAAGCATG AGAGGAAGCA AGAAAGAGGG CGATCCCAGG AGCGGAAGCA GCTCTCTTCC 
TCCTCTTCAG AGAAGCAGCG TTTCTATTCT TGTGACCGAT TTGGGAGCCG GGACCCATCA 
CAGCCCAAAT CCTCCAATGG AAGTCGTCCT ACATCACCTC CTGGGCAGGA GCCAGGTCCC 
CACAGACAGG GTAGTGGTTC AGTTAATGGG AGCCCCTTGC TGTCAACATC TGGTGCTAGT 
ACACCGGGCC GTGGTGGGCG GAGGCAGCTC CCCCAGACAC CTCTGACCCC CCGTCCCAGC 
ATCACATACA AGACTGCGAA CTCCTCGCCC GTCCACTTCA CCGGTTTCCA GACCAGCCTG 
CCCACCTTCT CCCCGGGACG GCTCAGTCGA GGCCTTTCTG AGCACAACAC CCTGCTCCAG 
AGGGATTCTC TGAGCCAGCC GCCAACCCCA GTGTCTCGGA TTGGCTCGGA CCCATACCTG 
GGGCACCGTG ACCACAGCGA TTCCTCGTAC CGGGCCCTGC CCGAGGACAC ACTCACTTTT 
GAGGAAGCCG TCGCCACCAA CTCGGGCCGC TCATCGAGGA CTTCCTACGT GTCCTCTCTA 
ACATCCCAGT CCCACCATCT CCGTCGTGTG CCCAATGGTT ACCACTGCAG TCTGGGGCTC 
AGCTCCGGCC CCGGGACTGG CGGTGGCAGG GCCCGGCAGA GTTATTATCA CCAAACCGAC 
GAAGATGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951865.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807725.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X16, mRNA.

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CloneID	OSa224814
Clone ID Related Accession (Same CDS sequence)	XM_031951865.1
Accession Version	XM_031951865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCAGG AAAAGGGAAA 
GACATCAGCA CAATCAAGTC ACTGCGAGTA CTCCGAGTCC TGAGACCTCT GAAGACCATC 
AAACGTTTGC CAAAACTAAA GGCTGTGTTT GACTGTGTGG TAAACTCCCT GAAGAATGTC 
CTCAATATCC TCATTGTCTA CATGCTCTTC ATGTTCATCT TTGCCGTCAT TGCTGTGCAG 
CTCTTCAAAG GAAAATTCTT CTATTGCACA GATGAATCCA AAGAGCTTCA GAGGGATTGC 
AGGGGTCAGT ATTTGGATTA TGAAAAGGAC GAAGTGGAGG CTCAACCCAG ACAATGGAAG 
AAATATGACT TTCACTATGA CAATGTGCTT TGGGCTTTGC TGACTCTTTT CACAGTGTCT 
ACAGGAGAAG GGTGGCCAAC GGTACTGAAG CATTCGGTGG ATGCAACATA TGAAGAACAG 
GGTCCAAGTC CTGGCTTCCG GATGGAACTG TCAATCTTCT ACGTGGTCTA CTTTGTTGTG 
TTTCCCTTTT TCTTCGTCAA TATCTTTGTG GCATTGATCA TCATCACCTT CCAGGAGCAG 
GGAGACAAAG TGATGTCAGA ATGCAGCTTG GAGAAGAATG AGAGGGCTTG CATAGACTTT 
GCCATCAGTG CCAAGCCCCT GACCCGTTAC ATGCCTCAGA ACAAGCAGTC CTTCCAGTAC 
AAGACATGGA CCTTTGTGGT GTCTCCACCC TTTGAGTATT TCATCATGGC CATGATCGCC 
CTCAACACGG CCGTCTTGAT GATGAAGTTC TATGGTGCCC CCTATGAGTA TGAAATGATG 
CTGAAGGGCT TGAATATTGT ATTCACTTCA ATGTTCTCTA TGGAGTGTGT GCTGAAGATC 
ATCGCCTTTG GGGTGCTGAA TTATTTCAGA GATGCCTGGA ATGTTTTTGA TTTTGTAACA 
GTATTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGGAAACGAA CAACTTCATC 
AATCTCAGCT TCTTGCGGCT CTTTCGGGCA GCCCGACTCA TTAAACTGCT TCGCCAGGGA 
TATACTATCC GAATTCTCCT GTGGACCTTT GTTCAGTCCT TCAAGGCTCT GCCGTATGTC 
TGCTTACTCA TCGCCATGCT CTTCTTCATC TACGCCATCA TTGGCATGCA GGTCTTTGGA 
AACATTGCTC TTGATGATGA AACGAATATC AACCGGCACA ACAATTTCCG GACATTCCTG 
CAAGCCCTGA TGCTGCTGTT CAGGAGTGCT ACAGGGGAAG CCTGGCATGA GATCATGTTG 
TCATGTCTTA GTGACCGTCC CTGTGATCCT GAAACCAAAC TAAAAGATGA ATGTGGGAGT 
GACTTTGCTT ACTTTTACTT CGTCTCCTTC ATTTTCCTCT GCTCCTTTCT GATGCTGAAT 
CTCTTTGTGG CAGTGATTAT GGATAATTTT GAGTACCTGA CTCGAGACTC TTCCATCCTA 
GGACCTCATC ACCTGGACGA ATTTATTCGG GTCTGGGCTG AATATGACCC AGCTGCATGT 
GGGCGCATCA GTTACAGTGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC ACCCCTCGGC 
CTGGGAAAGA AATGCCCTGC TCGAGTTGCC TATAAGCGCC TGGTGCGAAT GAACATGCCC 
ATCTCCAATG AGGACATGAC TGTGCACTTC ACTTCCACCT TGATGGCCCT CATCCGGACG 
GCTCTGGACA TCAAGCTTGC ACCAGCGGGG ATGAAACAGC ATCAGTGTGA TGCAGAACTG 
AGGAAAGAGA TTGCATCTGT TTGGGCTAAT CTGCCCCAGA AGACACTGGA CTTACTGGTG 
CCTCCACATA AACCTGATGA GATGACTGTA GGAAAAGTCT ACGCAGCTCT GATGATTTTT 
GACTTCTACA AGCAGAACAA AAACACCCGA GATCAAGGTC ACCAGCCTCC GACAGGTCCT 
GTGTCTCTGT TCCATACCCA AGGCCAACCA GCTGTGCTCC GAGGTGCTAG AGTGTTCTTG 
AAACAGAAAA GTTCAACCTC CCTAAGCAAT GGTGGAGCCT TACCACACCC AGGGAGTGGT 
ATCAAGGAGT CTGTCTCATG GGGCACCCAG CGCACCCAGG ATATCTGCTA TGAGACCAGG 
ATGCCCCTGG AACGTGGTCA TTCTGCAGAA ATTCCTGTGG GACAACCTAG CAAACAAGCT 
GTGGAGATGC AAGAAATGGC TCAGGATGCC ACCAGTGGAG AACCCCAGCC AGGGCTGGAA 
AGTCAGGGCC GAGCGGCCTC CATGCCCCGC CTGGCTGCAG AGATGCAGCC CATTCCAGAC 
ACAAGCCCCA TGAAGCGTTC CATCTCCACC TTGGCCCCAC AGCGCCCACA TGGCTCACAC 
CTTTATGAAT ATGGTCTGGA ACGCGTATCC TCAGGCCAGC CTCAGCATCA TCACCATCAT 
CGCTGTCACC GGCGCAGGGA TAAGAAGCAG AAGTCTTTGG ACAAATGCAC TAGTCAGTCA 
GCAGATGGAG ATGGGGCACC AAACCATAGT GTGGGGATGG CAGCTCCATC AGGGGAAGGT 
CCAGCCCCAT GTAAGCATGA GAGGAAGCAA GAAAGAGGGC GATCCCAGGA GCGGAAGCAG 
CTCTCTTCCT CCTCTTCAGA GAAGCAGCGT TTCTATTCTT GTGACCGATT TGGGAGCCGG 
GACCCATCAC AGCCCAAATC CTCCAATGGA AGTCGTCCTA CATCACCTCC TGGGCAGGAG 
CCAGGTCCCC ACAGACAGGG TAGTGGTTCA GTTAATGGGA GCCCCTTGCT GTCAACATCT 
GGTGCTAGTA CACCGGGCCG TGGTGGGCGG AGGCAGCTCC CCCAGACACC TCTGACCCCC 
CGTCCCAGCA TCACATACAA GACTGCGAAC TCCTCGCCCG TCCACTTCAC CGGTTTCCAG 
ACCAGCCTGC CCACCTTCTC CCCGGGACGG CTCAGTCGAG GCCTTTCTGA GCACAACACC 
CTGCTCCAGA GGGATTCTCT GAGCCAGCCG CCAACCCCAG TGTCTCGGAT TGGCTCGGAC 
CCATACCTGG GGCACCGTGA CCACAGCGAT TCCTCGTACC GGGCCCTGCC CGAGGACACA 
CTCACTTTTG AGGAAGCCGT CGCCACCAAC TCGGGCCGCT CATCGAGGAC TTCCTACGTG 
TCCTCTCTAA CATCCCAGTC CCACCATCTC CGTCGTGTGC CCAATGGTTA CCACTGCAGT 
CTGGGGCTCA GCTCCGGCCC CGGGACTGGC GGTGGCAGGG CCCGGCAGAG TTATTATCAC 
CAAACCGACG AAGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807725.1
CDS	1..7107
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAFVKQNRGTVSRSSSASSVNSPKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951865.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951865.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC TTTTGTAAAG 
CAAAATCGAG GCACTGTATC TCGCAGCTCA TCCGCCTCCA GCGTAAATTC ACCCAAGCAG 
CAGAACTCGG CCAAGGCCAG GTCTGTGTGG GAGCAGCGCA CGAGTCAGAT CCGGCTGCAC 
AATTTCCAGG CCAGCTGCGA GGCCCTGTAC AGCGAGATGG CCCCGGAGGA GCGCCTCCGC 
TACACCACCA CCCTCCACAT CCGTCCCGAC ATGAAGACGC ACCTGGACAG ACCCCTGGTG 
GTGGAGCCCC GCAGAGAAGA AGGGGTCCGG GCCCCGGGAG GCAAAGCGGG GACCGGGGAG 
GGGCTGGAGG CCTCGGAGCC GACCAAGGTG TCCCCCAGCG CGGCTGCGGA GGGCCCCGAG 
ATGCCCCGCA AGCACCACCG GCACAGGGAG AAGGAGAAGG AGAAGGAAAA GACAGGGGCT 
GGGGAGCAGG AGAAGGGAGA AGGTGTCAGG GAGGAAAATG GGGAGTCGGG GGTCGCCAGT 
AAGGAGGAGC GGCACCGGCA GCGCCGGAGC AGAAGCAAGG AGACTGGGGG CAACAAAGAG 
GGGAAATGCG AACGTTCCCG GGGCCAGGAG GGCGGCCGGA GGCACCACCG CCGGGGTTCC 
GTGGAGGACG TCACCGCCGA GAAGGAGCAC CGGCGGCACC GGGCCCACCG GCACTCAGCA 
GAGCCGCCGG GCAAAGAGGG CAACGGCACC GTCAGTGGGG CCAAGCCCGA GCGTCGGGCG 
CGCCATCGGG GGGGCTCCCG CTCGGGGACC CGGGAAGGAG AGCCTGGGCC CAAGGGAGAG 
AATGGAGAGG AGCCTCCCCG GAGGCATAAG ACCCGGCACA AAGCCCTCTC CATGTACGAC 
TCGGTGGAAA AGGAACCTGG AGAGAGGGAC GGGGAGCCTC CGGAGAAAGA GATGCGGAAT 
CACCAGCCCA AGGAGAACCA ATGTGAGGCG GAGGCCAGTG GTGGGATGGT GACCATCCCC 
ATTCACACAC TGCCCAGTAC CTGTCTGCAG AAGGTGAGCG AGCAGCCGGA AGACGCAGAT 
AACCAGAAGA ATGTGCATCG CATGACCCAA CCATCCCTGG AGACCCTCAC CATACGCATC 
CCAGTGACCC TGACTGCCCC TGGAGAGACC ACAGTGGTTC CAATGAACAA CGTTGACTTT 
GAAGGTAAAA TGGAAGAGAA GAAGGACGTA GAAGCTGGTG ACGTGACAAG AAGTGGACCC 
CGTCCAATTC TCCCCTATAG CTCCATGTTC TGCTTAAGCC CCACCAACCT ACTCCGGCGT 
GCCTGCCACT ACATAGTGAA TATGCGTTAC TTTGAAATGG TCATCCTCGG GGTCATCGCC 
CTCAGCAGCA TTGCTCTGGC TGCTGAGGAT CCAGTACAGG CCGACTCGCC CAGGAACAAC 
GTTCTCAAGT ACATGGATTA CATTTTCACT GGGGTCTTCA CCTTTGAGAT GGTAATCAAG 
ATGATTGACC TGGGGCTGCT GCTTCATCCT GGCTCTTATT TCCGAGACCT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGGGCCCTG GTGGCTTTTG CCTTCTCAGG AAAAGGGAAA 
GACATCAGCA CAATCAAGTC ACTGCGAGTA CTCCGAGTCC TGAGACCTCT GAAGACCATC 
AAACGTTTGC CAAAACTAAA GGCTGTGTTT GACTGTGTGG TAAACTCCCT GAAGAATGTC 
CTCAATATCC TCATTGTCTA CATGCTCTTC ATGTTCATCT TTGCCGTCAT TGCTGTGCAG 
CTCTTCAAAG GAAAATTCTT CTATTGCACA GATGAATCCA AAGAGCTTCA GAGGGATTGC 
AGGGGTCAGT ATTTGGATTA TGAAAAGGAC GAAGTGGAGG CTCAACCCAG ACAATGGAAG 
AAATATGACT TTCACTATGA CAATGTGCTT TGGGCTTTGC TGACTCTTTT CACAGTGTCT 
ACAGGAGAAG GGTGGCCAAC GGTACTGAAG CATTCGGTGG ATGCAACATA TGAAGAACAG 
GGTCCAAGTC CTGGCTTCCG GATGGAACTG TCAATCTTCT ACGTGGTCTA CTTTGTTGTG 
TTTCCCTTTT TCTTCGTCAA TATCTTTGTG GCATTGATCA TCATCACCTT CCAGGAGCAG 
GGAGACAAAG TGATGTCAGA ATGCAGCTTG GAGAAGAATG AGAGGGCTTG CATAGACTTT 
GCCATCAGTG CCAAGCCCCT GACCCGTTAC ATGCCTCAGA ACAAGCAGTC CTTCCAGTAC 
AAGACATGGA CCTTTGTGGT GTCTCCACCC TTTGAGTATT TCATCATGGC CATGATCGCC 
CTCAACACGG CCGTCTTGAT GATGAAGTTC TATGGTGCCC CCTATGAGTA TGAAATGATG 
CTGAAGGGCT TGAATATTGT ATTCACTTCA ATGTTCTCTA TGGAGTGTGT GCTGAAGATC 
ATCGCCTTTG GGGTGCTGAA TTATTTCAGA GATGCCTGGA ATGTTTTTGA TTTTGTAACA 
GTATTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGGAAACGAA CAACTTCATC 
AATCTCAGCT TCTTGCGGCT CTTTCGGGCA GCCCGACTCA TTAAACTGCT TCGCCAGGGA 
TATACTATCC GAATTCTCCT GTGGACCTTT GTTCAGTCCT TCAAGGCTCT GCCGTATGTC 
TGCTTACTCA TCGCCATGCT CTTCTTCATC TACGCCATCA TTGGCATGCA GGTCTTTGGA 
AACATTGCTC TTGATGATGA AACGAATATC AACCGGCACA ACAATTTCCG GACATTCCTG 
CAAGCCCTGA TGCTGCTGTT CAGGAGTGCT ACAGGGGAAG CCTGGCATGA GATCATGTTG 
TCATGTCTTA GTGACCGTCC CTGTGATCCT GAAACCAAAC TAAAAGATGA ATGTGGGAGT 
GACTTTGCTT ACTTTTACTT CGTCTCCTTC ATTTTCCTCT GCTCCTTTCT GATGCTGAAT 
CTCTTTGTGG CAGTGATTAT GGATAATTTT GAGTACCTGA CTCGAGACTC TTCCATCCTA 
GGACCTCATC ACCTGGACGA ATTTATTCGG GTCTGGGCTG AATATGACCC AGCTGCATGT 
GGGCGCATCA GTTACAGTGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC ACCCCTCGGC 
CTGGGAAAGA AATGCCCTGC TCGAGTTGCC TATAAGCGCC TGGTGCGAAT GAACATGCCC 
ATCTCCAATG AGGACATGAC TGTGCACTTC ACTTCCACCT TGATGGCCCT CATCCGGACG 
GCTCTGGACA TCAAGCTTGC ACCAGCGGGG ATGAAACAGC ATCAGTGTGA TGCAGAACTG 
AGGAAAGAGA TTGCATCTGT TTGGGCTAAT CTGCCCCAGA AGACACTGGA CTTACTGGTG 
CCTCCACATA AACCTGATGA GATGACTGTA GGAAAAGTCT ACGCAGCTCT GATGATTTTT 
GACTTCTACA AGCAGAACAA AAACACCCGA GATCAAGGTC ACCAGCCTCC GACAGGTCCT 
GTGTCTCTGT TCCATACCCA AGGCCAACCA GCTGTGCTCC GAGGTGCTAG AGTGTTCTTG 
AAACAGAAAA GTTCAACCTC CCTAAGCAAT GGTGGAGCCT TACCACACCC AGGGAGTGGT 
ATCAAGGAGT CTGTCTCATG GGGCACCCAG CGCACCCAGG ATATCTGCTA TGAGACCAGG 
ATGCCCCTGG AACGTGGTCA TTCTGCAGAA ATTCCTGTGG GACAACCTAG CAAACAAGCT 
GTGGAGATGC AAGAAATGGC TCAGGATGCC ACCAGTGGAG AACCCCAGCC AGGGCTGGAA 
AGTCAGGGCC GAGCGGCCTC CATGCCCCGC CTGGCTGCAG AGATGCAGCC CATTCCAGAC 
ACAAGCCCCA TGAAGCGTTC CATCTCCACC TTGGCCCCAC AGCGCCCACA TGGCTCACAC 
CTTTATGAAT ATGGTCTGGA ACGCGTATCC TCAGGCCAGC CTCAGCATCA TCACCATCAT 
CGCTGTCACC GGCGCAGGGA TAAGAAGCAG AAGTCTTTGG ACAAATGCAC TAGTCAGTCA 
GCAGATGGAG ATGGGGCACC AAACCATAGT GTGGGGATGG CAGCTCCATC AGGGGAAGGT 
CCAGCCCCAT GTAAGCATGA GAGGAAGCAA GAAAGAGGGC GATCCCAGGA GCGGAAGCAG 
CTCTCTTCCT CCTCTTCAGA GAAGCAGCGT TTCTATTCTT GTGACCGATT TGGGAGCCGG 
GACCCATCAC AGCCCAAATC CTCCAATGGA AGTCGTCCTA CATCACCTCC TGGGCAGGAG 
CCAGGTCCCC ACAGACAGGG TAGTGGTTCA GTTAATGGGA GCCCCTTGCT GTCAACATCT 
GGTGCTAGTA CACCGGGCCG TGGTGGGCGG AGGCAGCTCC CCCAGACACC TCTGACCCCC 
CGTCCCAGCA TCACATACAA GACTGCGAAC TCCTCGCCCG TCCACTTCAC CGGTTTCCAG 
ACCAGCCTGC CCACCTTCTC CCCGGGACGG CTCAGTCGAG GCCTTTCTGA GCACAACACC 
CTGCTCCAGA GGGATTCTCT GAGCCAGCCG CCAACCCCAG TGTCTCGGAT TGGCTCGGAC 
CCATACCTGG GGCACCGTGA CCACAGCGAT TCCTCGTACC GGGCCCTGCC CGAGGACACA 
CTCACTTTTG AGGAAGCCGT CGCCACCAAC TCGGGCCGCT CATCGAGGAC TTCCTACGTG 
TCCTCTCTAA CATCCCAGTC CCACCATCTC CGTCGTGTGC CCAATGGTTA CCACTGCAGT 
CTGGGGCTCA GCTCCGGCCC CGGGACTGGC GGTGGCAGGG CCCGGCAGAG TTATTATCAC 
CAAACCGACG AAGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951866.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807726.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X17, mRNA.

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CloneID	OSa224815
Clone ID Related Accession (Same CDS sequence)	XM_031951866.1
Accession Version	XM_031951866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCGAGCTT TATGGGAGGA 
AAAGGGAAAG ACATCAGCAC AATCAAGTCA CTGCGAGTAC TCCGAGTCCT GAGACCTCTG 
AAGACCATCA AACGTTTGCC AAAACTAAAG GCTGTGTTTG ACTGTGTGGT AAACTCCCTG 
AAGAATGTCC TCAATATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCCGTCATT 
GCTGTGCAGC TCTTCAAAGG AAAATTCTTC TATTGCACAG ATGAATCCAA AGAGCTTCAG 
AGGGATTGCA GGGGTCAGTA TTTGGATTAT GAAAAGGACG AAGTGGAGGC TCAACCCAGA 
CAATGGAAGA AATATGACTT TCACTATGAC AATGTGCTTT GGGCTTTGCT GACTCTTTTC 
ACAGTGTCTA CAGGAGAAGG GTGGCCAACG GTACTGAAGC ATTCGGTGGA TGCAACATAT 
GAAGAACAGG GTCCAAGTCC TGGCTTCCGG ATGGAACTGT CAATCTTCTA CGTGGTCTAC 
TTTGTTGTGT TTCCCTTTTT CTTCGTCAAT ATCTTTGTGG CATTGATCAT CATCACCTTC 
CAGGAGCAGG GAGACAAAGT GATGTCAGAA TGCAGCTTGG AGAAGAATGA GAGGGCTTGC 
ATAGACTTTG CCATCAGTGC CAAGCCCCTG ACCCGTTACA TGCCTCAGAA CAAGCAGTCC 
TTCCAGTACA AGACATGGAC CTTTGTGGTG TCTCCACCCT TTGAGTATTT CATCATGGCC 
ATGATCGCCC TCAACACGGC CGTCTTGATG ATGAAGTTCT ATGGTGCCCC CTATGAGTAT 
GAAATGATGC TGAAGGGCTT GAATATTGTA TTCACTTCAA TGTTCTCTAT GGAGTGTGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAT TATTTCAGAG ATGCCTGGAA TGTTTTTGAT 
TTTGTAACAG TATTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GAACAACTTC 
ATCAATCTCA GCTTCTTGCG GCTCTTTCGG GCAGCCCGAC TCATTAAACT GCTTCGCCAG 
GGATATACTA TCCGAATTCT CCTGTGGACC TTTGTTCAGT CCTTCAAGGC TCTGCCGTAT 
GTCTGCTTAC TCATCGCCAT GCTCTTCTTC ATCTACGCCA TCATTGGCAT GCAGGTCTTT 
GGAAACATTG CTCTTGATGA TGAAACGAAT ATCAACCGGC ACAACAATTT CCGGACATTC 
CTGCAAGCCC TGATGCTGCT GTTCAGGAGT GCTACAGGGG AAGCCTGGCA TGAGATCATG 
TTGTCATGTC TTAGTGACCG TCCCTGTGAT CCTGAAACCA AACTAAAAGA TGAATGTGGG 
AGTGACTTTG CTTACTTTTA CTTCGTCTCC TTCATTTTCC TCTGCTCCTT TCTGATGCTG 
AATCTCTTTG TGGCAGTGAT TATGGATAAT TTTGAGTACC TGACTCGAGA CTCTTCCATC 
CTAGGACCTC ATCACCTGGA CGAATTTATT CGGGTCTGGG CTGAATATGA CCCAGCTGCA 
TGTGGGCGCA TCAGTTACAG TGACATGTTT GAGATGCTGA AACACATGTC CCCACCCCTC 
GGCCTGGGAA AGAAATGCCC TGCTCGAGTT GCCTATAAGC GCCTGGTGCG AATGAACATG 
CCCATCTCCA ATGAGGACAT GACTGTGCAC TTCACTTCCA CCTTGATGGC CCTCATCCGG 
ACGGCTCTGG ACATCAAGCT TGCACCAGCG GGGATGAAAC AGCATCAGTG TGATGCAGAA 
CTGAGGAAAG AGATTGCATC TGTTTGGGCT AATCTGCCCC AGAAGACACT GGACTTACTG 
GTGCCTCCAC ATAAACCTGA TGAGATGACT GTAGGAAAAG TCTACGCAGC TCTGATGATT 
TTTGACTTCT ACAAGCAGAA CAAAAACACC CGAGATCAAG GTCACCAGCC TCCGACAGGT 
CCTGTGTCTC TGTTCCATAC CCAAGGCCAA CCAGCTGTGC TCCGAGGTGC TAGAGTGTTC 
TTGAAACAGA AAAGTTCAAC CTCCCTAAGC AATGGTGGAG CCTTACCACA CCCAGGGAGT 
GGTATCAAGG AGTCTGTCTC ATGGGGCACC CAGCGCACCC AGGATATCTG CTATGAGACC 
AGGATGCCCC TGGAACGTGG TCATTCTGCA GAAATTCCTG TGGGACAACC TAGCAAACAA 
GCTGTGGAGA TGCAAGAAAT GGCTCAGGAT GCCACCAGTG GAGAACCCCA GCCAGGGCTG 
GAAAGTCAGG GCCGAGCGGC CTCCATGCCC CGCCTGGCTG CAGAGATGCA GCCCATTCCA 
GACACAAGCC CCATGAAGCG TTCCATCTCC ACCTTGGCCC CACAGCGCCC ACATGGCTCA 
CACCTTTATG AATATGGTCT GGAACGCGTA TCCTCAGGCC AGCCTCAGCA TCATCACCAT 
CATCGCTGTC ACCGGCGCAG GGATAAGAAG CAGAAGTCTT TGGACAAATG CACTAGTCAG 
TCAGCAGATG GAGATGGGGC ACCAAACCAT AGTGTGGGGA TGGCAGCTCC ATCAGGGGAA 
GGTCCAGCCC CATGTAAGCA TGAGAGGAAG CAAGAAAGAG GGCGATCCCA GGAGCGGAAG 
CAGCTCTCTT CCTCCTCTTC AGAGAAGCAG CGTTTCTATT CTTGTGACCG ATTTGGGAGC 
CGGGACCCAT CACAGCCCAA ATCCTCCAAT GGAAGTCGTC CTACATCACC TCCTGGGCAG 
GAGCCAGGTC CCCACAGACA GGGTAGTGGT TCAGTTAATG GGAGCCCCTT GCTGTCAACA 
TCTGGTGCTA GTACACCGGG CCGTGGTGGG CGGAGGCAGC TCCCCCAGAC ACCTCTGACC 
CCCCGTCCCA GCATCACATA CAAGACTGCG AACTCCTCGC CCGTCCACTT CACCGGTTTC 
CAGACCAGCC TGCCCACCTT CTCCCCGGGA CGGCTCAGTC GAGGCCTTTC TGAGCACAAC 
ACCCTGCTCC AGAGGGATTC TCTGAGCCAG CCGCCAACCC CAGTGTCTCG GATTGGCTCG 
GACCCATACC TGGGGCACCG TGACCACAGC GATTCCTCGT ACCGGGCCCT GCCCGAGGAC 
ACACTCACTT TTGAGGAAGC CGTCGCCACC AACTCGGGCC GCTCATCGAG GACTTCCTAC 
GTGTCCTCTC TAACATCCCA GTCCCACCAT CTCCGTCGTG TGCCCAATGG TTACCACTGC 
AGTCTGGGGC TCAGCTCCGG CCCCGGGACT GGCGGTGGCA GGGCCCGGCA GAGTTATTAT 
CACCAAACCG ACGAAGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807726.1
CDS	1..7050
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDAVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951866.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951866.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGCAGTGCT GAAGAGAGCA 
GCCACCAAGA AAAGTAAAAA TGATTTGATC CATGCTGAAG AAGGGGACGA TCGCTTCGCA 
GACCTCTGCT CTGTGGGTTC TCCGTTTGCT AGAGCTAGTT TGAAGAGTGG GAAGAATGAC 
AGCTCCTCCT ATTTCCGGAG GAAAGAGAAG ATGTTCCGCT TCTTCATCCG TCGCATGGTG 
AAAGCCCAGA CCTTCTACTG GATCGTCCTC TGTGTCGTGG CATTGAACAC ACTGTGTGTG 
GCTATGGTGC ACTATGACCA GCCACAAAGA CTCACCAATG CTTTGTATTT TGCAGAATTT 
GTTTTCCTGG GTCTCTTCCT CACTGAGATG TCTCTGAAGA TGTATGGCCT GGGGCCCAGA 
AATTATTTCC ATTCATCCTT CAACTGCTTT GACTTTGGGG TCATCGTTGG AAGCATTTTT 
GAAGTCATCT GGGCAGCGAT CAAGCCGGGG ACTTCATTTG GAATCAGTGT GCTGAGGGCT 
CTCCGGCTGC TGAGGATTTT CAAAGTCACA AAGTATTGGA ATTCCCTTCG GAACTTGGTG 
GTCTCCTTGC TGAACTCCAT GAAATCCATC ATCAGCCTGC TCTTCCTTCT CTTTCTGTTC 
ATTGTGGTTT TCGCCCTGCT GGGGATGCAG CTCTTTGGAG GACAGTTCAA TTTTAGAGAC 
GAGACCCCGA ACACCAACTT TGACACTTTT CCGGCTGCAA TTCTCACTGT ATTCCAGATC 
CTTACTGGAG AAGACTGGAA CGTGGTCATG TATCATGGGA TAGAGTCCCA AGGGGGAGTC 
AGCAAAGGAA TGTTCTCTTC TTTCTATTTT ATCATCCTGA CACTGTTCGG AAACTACACC 
CTTCTGAATG TCTTCCTGGC TATCGCTGTG GACAATCTTG CCAATGCCCA GGAACTGACC 
AAGGATGAGG AAGAATTGGA GGAAGCAGCC AATCAGAAGC TTGCTTTGCA GAAGGCCAAG 
GAGGTCGCTG AAGTCAGCCC CCTGTCTGCT GCCAACATCT CCATAGCAGC CAAGCAGCAG 
AACTCGGCCA AGGCCAGGTC TGTGTGGGAG CAGCGCACGA GTCAGATCCG GCTGCACAAT 
TTCCAGGCCA GCTGCGAGGC CCTGTACAGC GAGATGGCCC CGGAGGAGCG CCTCCGCTAC 
ACCACCACCC TCCACATCCG TCCCGACATG AAGACGCACC TGGACAGACC CCTGGTGGTG 
GAGCCCCGCA GAGAAGAAGG GGTCCGGGCC CCGGGAGGCA AAGCGGGGAC CGGGGAGGGG 
CTGGAGGCCT CGGAGCCGAC CAAGGTGTCC CCCAGCGCGG CTGCGGAGGG CCCCGAGATG 
CCCCGCAAGC ACCACCGGCA CAGGGAGAAG GAGAAGGAGA AGGAAAAGAC AGGGGCTGGG 
GAGCAGGAGA AGGGAGAAGG TGTCAGGGAG GAAAATGGGG AGTCGGGGGT CGCCAGTAAG 
GAGGAGCGGC ACCGGCAGCG CCGGAGCAGA AGCAAGGAGA CTGGGGGCAA CAAAGAGGGG 
AAATGCGAAC GTTCCCGGGG CCAGGAGGGC GGCCGGAGGC ACCACCGCCG GGGTTCCGTG 
GAGGACGTCA CCGCCGAGAA GGAGCACCGG CGGCACCGGG CCCACCGGCA CTCAGCAGAG 
CCGCCGGGCA AAGAGGGCAA CGGCACCGTC AGTGGGGCCA AGCCCGAGCG TCGGGCGCGC 
CATCGGGGGG GCTCCCGCTC GGGGACCCGG GAAGGAGAGC CTGGGCCCAA GGGAGAGAAT 
GGAGAGGAGC CTCCCCGGAG GCATAAGACC CGGCACAAAG CCCTCTCCAT GTACGACTCG 
GTGGAAAAGG AACCTGGAGA GAGGGACGGG GAGCCTCCGG AGAAAGAGAT GCGGAATCAC 
CAGCCCAAGG AGAACCAATG TGAGGCGGAG GCCAGTGGTG GGATGGTGAC CATCCCCATT 
CACACACTGC CCAGTACCTG TCTGCAGAAG GTGAGCGAGC AGCCGGAAGA CGCAGATAAC 
CAGAAGAATG TGCATCGCAT GACCCAACCA TCCCTGGAGA CCCTCACCAT ACGCATCCCA 
GTGACCCTGA CTGCCCCTGG AGAGACCACA GTGGTTCCAA TGAACAACGT TGACTTTGAA 
GGTAAAATGG AAGAGAAGAA GGACGTAGAA GCTGGTGACG TGACAAGAAG TGGACCCCGT 
CCAATTCTCC CCTATAGCTC CATGTTCTGC TTAAGCCCCA CCAACCTACT CCGGCGTGCC 
TGCCACTACA TAGTGAATAT GCGTTACTTT GAAATGGTCA TCCTCGGGGT CATCGCCCTC 
AGCAGCATTG CTCTGGCTGC TGAGGATCCA GTACAGGCCG ACTCGCCCAG GAACAACGTT 
CTCAAGTACA TGGATTACAT TTTCACTGGG GTCTTCACCT TTGAGATGGT AATCAAGATG 
ATTGACCTGG GGCTGCTGCT TCATCCTGGC TCTTATTTCC GAGACCTGTG GAACATTCTG 
GACTTCATTG TGGTCAGTGG GGCCCTGGTG GCTTTTGCCT TCTCGAGCTT TATGGGAGGA 
AAAGGGAAAG ACATCAGCAC AATCAAGTCA CTGCGAGTAC TCCGAGTCCT GAGACCTCTG 
AAGACCATCA AACGTTTGCC AAAACTAAAG GCTGTGTTTG ACTGTGTGGT AAACTCCCTG 
AAGAATGTCC TCAATATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCCGTCATT 
GCTGTGCAGC TCTTCAAAGG AAAATTCTTC TATTGCACAG ATGAATCCAA AGAGCTTCAG 
AGGGATTGCA GGGGTCAGTA TTTGGATTAT GAAAAGGACG AAGTGGAGGC TCAACCCAGA 
CAATGGAAGA AATATGACTT TCACTATGAC AATGTGCTTT GGGCTTTGCT GACTCTTTTC 
ACAGTGTCTA CAGGAGAAGG GTGGCCAACG GTACTGAAGC ATTCGGTGGA TGCAACATAT 
GAAGAACAGG GTCCAAGTCC TGGCTTCCGG ATGGAACTGT CAATCTTCTA CGTGGTCTAC 
TTTGTTGTGT TTCCCTTTTT CTTCGTCAAT ATCTTTGTGG CATTGATCAT CATCACCTTC 
CAGGAGCAGG GAGACAAAGT GATGTCAGAA TGCAGCTTGG AGAAGAATGA GAGGGCTTGC 
ATAGACTTTG CCATCAGTGC CAAGCCCCTG ACCCGTTACA TGCCTCAGAA CAAGCAGTCC 
TTCCAGTACA AGACATGGAC CTTTGTGGTG TCTCCACCCT TTGAGTATTT CATCATGGCC 
ATGATCGCCC TCAACACGGC CGTCTTGATG ATGAAGTTCT ATGGTGCCCC CTATGAGTAT 
GAAATGATGC TGAAGGGCTT GAATATTGTA TTCACTTCAA TGTTCTCTAT GGAGTGTGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAT TATTTCAGAG ATGCCTGGAA TGTTTTTGAT 
TTTGTAACAG TATTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GAACAACTTC 
ATCAATCTCA GCTTCTTGCG GCTCTTTCGG GCAGCCCGAC TCATTAAACT GCTTCGCCAG 
GGATATACTA TCCGAATTCT CCTGTGGACC TTTGTTCAGT CCTTCAAGGC TCTGCCGTAT 
GTCTGCTTAC TCATCGCCAT GCTCTTCTTC ATCTACGCCA TCATTGGCAT GCAGGTCTTT 
GGAAACATTG CTCTTGATGA TGAAACGAAT ATCAACCGGC ACAACAATTT CCGGACATTC 
CTGCAAGCCC TGATGCTGCT GTTCAGGAGT GCTACAGGGG AAGCCTGGCA TGAGATCATG 
TTGTCATGTC TTAGTGACCG TCCCTGTGAT CCTGAAACCA AACTAAAAGA TGAATGTGGG 
AGTGACTTTG CTTACTTTTA CTTCGTCTCC TTCATTTTCC TCTGCTCCTT TCTGATGCTG 
AATCTCTTTG TGGCAGTGAT TATGGATAAT TTTGAGTACC TGACTCGAGA CTCTTCCATC 
CTAGGACCTC ATCACCTGGA CGAATTTATT CGGGTCTGGG CTGAATATGA CCCAGCTGCA 
TGTGGGCGCA TCAGTTACAG TGACATGTTT GAGATGCTGA AACACATGTC CCCACCCCTC 
GGCCTGGGAA AGAAATGCCC TGCTCGAGTT GCCTATAAGC GCCTGGTGCG AATGAACATG 
CCCATCTCCA ATGAGGACAT GACTGTGCAC TTCACTTCCA CCTTGATGGC CCTCATCCGG 
ACGGCTCTGG ACATCAAGCT TGCACCAGCG GGGATGAAAC AGCATCAGTG TGATGCAGAA 
CTGAGGAAAG AGATTGCATC TGTTTGGGCT AATCTGCCCC AGAAGACACT GGACTTACTG 
GTGCCTCCAC ATAAACCTGA TGAGATGACT GTAGGAAAAG TCTACGCAGC TCTGATGATT 
TTTGACTTCT ACAAGCAGAA CAAAAACACC CGAGATCAAG GTCACCAGCC TCCGACAGGT 
CCTGTGTCTC TGTTCCATAC CCAAGGCCAA CCAGCTGTGC TCCGAGGTGC TAGAGTGTTC 
TTGAAACAGA AAAGTTCAAC CTCCCTAAGC AATGGTGGAG CCTTACCACA CCCAGGGAGT 
GGTATCAAGG AGTCTGTCTC ATGGGGCACC CAGCGCACCC AGGATATCTG CTATGAGACC 
AGGATGCCCC TGGAACGTGG TCATTCTGCA GAAATTCCTG TGGGACAACC TAGCAAACAA 
GCTGTGGAGA TGCAAGAAAT GGCTCAGGAT GCCACCAGTG GAGAACCCCA GCCAGGGCTG 
GAAAGTCAGG GCCGAGCGGC CTCCATGCCC CGCCTGGCTG CAGAGATGCA GCCCATTCCA 
GACACAAGCC CCATGAAGCG TTCCATCTCC ACCTTGGCCC CACAGCGCCC ACATGGCTCA 
CACCTTTATG AATATGGTCT GGAACGCGTA TCCTCAGGCC AGCCTCAGCA TCATCACCAT 
CATCGCTGTC ACCGGCGCAG GGATAAGAAG CAGAAGTCTT TGGACAAATG CACTAGTCAG 
TCAGCAGATG GAGATGGGGC ACCAAACCAT AGTGTGGGGA TGGCAGCTCC ATCAGGGGAA 
GGTCCAGCCC CATGTAAGCA TGAGAGGAAG CAAGAAAGAG GGCGATCCCA GGAGCGGAAG 
CAGCTCTCTT CCTCCTCTTC AGAGAAGCAG CGTTTCTATT CTTGTGACCG ATTTGGGAGC 
CGGGACCCAT CACAGCCCAA ATCCTCCAAT GGAAGTCGTC CTACATCACC TCCTGGGCAG 
GAGCCAGGTC CCCACAGACA GGGTAGTGGT TCAGTTAATG GGAGCCCCTT GCTGTCAACA 
TCTGGTGCTA GTACACCGGG CCGTGGTGGG CGGAGGCAGC TCCCCCAGAC ACCTCTGACC 
CCCCGTCCCA GCATCACATA CAAGACTGCG AACTCCTCGC CCGTCCACTT CACCGGTTTC 
CAGACCAGCC TGCCCACCTT CTCCCCGGGA CGGCTCAGTC GAGGCCTTTC TGAGCACAAC 
ACCCTGCTCC AGAGGGATTC TCTGAGCCAG CCGCCAACCC CAGTGTCTCG GATTGGCTCG 
GACCCATACC TGGGGCACCG TGACCACAGC GATTCCTCGT ACCGGGCCCT GCCCGAGGAC 
ACACTCACTT TTGAGGAAGC CGTCGCCACC AACTCGGGCC GCTCATCGAG GACTTCCTAC 
GTGTCCTCTC TAACATCCCA GTCCCACCAT CTCCGTCGTG TGCCCAATGG TTACCACTGC 
AGTCTGGGGC TCAGCTCCGG CCCCGGGACT GGCGGTGGCA GGGCCCGGCA GAGTTATTAT 
CACCAAACCG ACGAAGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_031951867.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807727.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X18, mRNA.

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CloneID	OSa224816
Clone ID Related Accession (Same CDS sequence)	XM_031951867.1
Accession Version	XM_031951867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CGAGCTTTAT GGGAGGAAAA 
GGGAAAGACA TCAGCACAAT CAAGTCACTG CGAGTACTCC GAGTCCTGAG ACCTCTGAAG 
ACCATCAAAC GTTTGCCAAA ACTAAAGGCT GTGTTTGACT GTGTGGTAAA CTCCCTGAAG 
AATGTCCTCA ATATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC CGTCATTGCT 
GTGCAGCTCT TCAAAGGAAA ATTCTTCTAT TGCACAGATG AATCCAAAGA GCTTCAGAGG 
GATTGCAGGG GTCAGTATTT GGATTATGAA AAGGACGAAG TGGAGGCTCA ACCCAGACAA 
TGGAAGAAAT ATGACTTTCA CTATGACAAT GTGCTTTGGG CTTTGCTGAC TCTTTTCACA 
GTGTCTACAG GAGAAGGGTG GCCAACGGTA CTGAAGCATT CGGTGGATGC AACATATGAA 
GAACAGGGTC CAAGTCCTGG CTTCCGGATG GAACTGTCAA TCTTCTACGT GGTCTACTTT 
GTTGTGTTTC CCTTTTTCTT CGTCAATATC TTTGTGGCAT TGATCATCAT CACCTTCCAG 
GAGCAGGGAG ACAAAGTGAT GTCAGAATGC AGCTTGGAGA AGAATGAGAG GGCTTGCATA 
GACTTTGCCA TCAGTGCCAA GCCCCTGACC CGTTACATGC CTCAGAACAA GCAGTCCTTC 
CAGTACAAGA CATGGACCTT TGTGGTGTCT CCACCCTTTG AGTATTTCAT CATGGCCATG 
ATCGCCCTCA ACACGGCCGT CTTGATGATG AAGTTCTATG GTGCCCCCTA TGAGTATGAA 
ATGATGCTGA AGGGCTTGAA TATTGTATTC ACTTCAATGT TCTCTATGGA GTGTGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAATTAT TTCAGAGATG CCTGGAATGT TTTTGATTTT 
GTAACAGTAT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGAA CAACTTCATC 
AATCTCAGCT TCTTGCGGCT CTTTCGGGCA GCCCGACTCA TTAAACTGCT TCGCCAGGGA 
TATACTATCC GAATTCTCCT GTGGACCTTT GTTCAGTCCT TCAAGGCTCT GCCGTATGTC 
TGCTTACTCA TCGCCATGCT CTTCTTCATC TACGCCATCA TTGGCATGCA GGTCTTTGGA 
AACATTGCTC TTGATGATGA AACGAATATC AACCGGCACA ACAATTTCCG GACATTCCTG 
CAAGCCCTGA TGCTGCTGTT CAGGAGTGCT ACAGGGGAAG CCTGGCATGA GATCATGTTG 
TCATGTCTTA GTGACCGTCC CTGTGATCCT GAAACCAAAC TAAAAGATGA ATGTGGGAGT 
GACTTTGCTT ACTTTTACTT CGTCTCCTTC ATTTTCCTCT GCTCCTTTCT GATGCTGAAT 
CTCTTTGTGG CAGTGATTAT GGATAATTTT GAGTACCTGA CTCGAGACTC TTCCATCCTA 
GGACCTCATC ACCTGGACGA ATTTATTCGG GTCTGGGCTG AATATGACCC AGCTGCATGT 
GGGCGCATCA GTTACAGTGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC ACCCCTCGGC 
CTGGGAAAGA AATGCCCTGC TCGAGTTGCC TATAAGCGCC TGGTGCGAAT GAACATGCCC 
ATCTCCAATG AGGACATGAC TGTGCACTTC ACTTCCACCT TGATGGCCCT CATCCGGACG 
GCTCTGGACA TCAAGCTTGC ACCAGCGGGG ATGAAACAGC ATCAGTGTGA TGCAGAACTG 
AGGAAAGAGA TTGCATCTGT TTGGGCTAAT CTGCCCCAGA AGACACTGGA CTTACTGGTG 
CCTCCACATA AACCTGATGA GATGACTGTA GGAAAAGTCT ACGCAGCTCT GATGATTTTT 
GACTTCTACA AGCAGAACAA AAACACCCGA GATCAAGGTC ACCAGCCTCC GACAGGTCCT 
GTGTCTCTGT TCCATACCCA AGGCCAACCA GCTGTGCTCC GAGGTGCTAG AGTGTTCTTG 
AAACAGAAAA GTTCAACCTC CCTAAGCAAT GGTGGAGCCT TACCACACCC AGGGAGTGGT 
ATCAAGGAGT CTGTCTCATG GGGCACCCAG CGCACCCAGG ATATCTGCTA TGAGACCAGG 
ATGCCCCTGG AACGTGGTCA TTCTGCAGAA ATTCCTGTGG GACAACCTAG CAAACAAGCT 
GTGGAGATGC AAGAAATGGC TCAGGATGCC ACCAGTGGAG AACCCCAGCC AGGGCTGGAA 
AGTCAGGGCC GAGCGGCCTC CATGCCCCGC CTGGCTGCAG AGATGCAGCC CATTCCAGAC 
ACAAGCCCCA TGAAGCGTTC CATCTCCACC TTGGCCCCAC AGCGCCCACA TGGCTCACAC 
CTTTATGAAT ATGGTCTGGA ACGCGTATCC TCAGGCCAGC CTCAGCATCA TCACCATCAT 
CGCTGTCACC GGCGCAGGGA TAAGAAGCAG AAGTCTTTGG ACAAATGCAC TAGTCAGTCA 
GCAGATGGAG ATGGGGCACC AAACCATAGT GTGGGGATGG CAGCTCCATC AGGGGAAGGT 
CCAGCCCCAT GTAAGCATGA GAGGAAGCAA GAAAGAGGGC GATCCCAGGA GCGGAAGCAG 
CTCTCTTCCT CCTCTTCAGA GAAGCAGCGT TTCTATTCTT GTGACCGATT TGGGAGCCGG 
GACCCATCAC AGCCCAAATC CTCCAATGGA AGTCGTCCTA CATCACCTCC TGGGCAGGAG 
CCAGGTCCCC ACAGACAGGG TAGTGGTTCA GTTAATGGGA GCCCCTTGCT GTCAACATCT 
GGTGCTAGTA CACCGGGCCG TGGTGGGCGG AGGCAGCTCC CCCAGACACC TCTGACCCCC 
CGTCCCAGCA TCACATACAA GACTGCGAAC TCCTCGCCCG TCCACTTCAC CGGTTTCCAG 
ACCAGCCTGC CCACCTTCTC CCCGGGACGG CTCAGTCGAG GCCTTTCTGA GCACAACACC 
CTGCTCCAGA GGGATTCTCT GAGCCAGCCG CCAACCCCAG TGTCTCGGAT TGGCTCGGAC 
CCATACCTGG GGCACCGTGA CCACAGCGAT TCCTCGTACC GGGCCCTGCC CGAGGACACA 
CTCACTTTTG AGGAAGCCGT CGCCACCAAC TCGGGCCGCT CATCGAGGAC TTCCTACGTG 
TCCTCTCTAA CATCCCAGTC CCACCATCTC CGTCGTGTGC CCAATGGTTA CCACTGCAGT 
CTGGGGCTCA GCTCCGGCCC CGGGACTGGC GGTGGCAGGG CCCGGCAGAG TTATTATCAC 
CAAACCGACG AAGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807727.1
CDS	1..7047
Translation	MVRFGDELPGRYGGAGPAGGGRGGGGAGAGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVVRRYAKRVTEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKACFPNNTADPHSDPVGDFPCGTDASARQCENGYICREYWRGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNADEKSPLDVLKRAATKKSKNDLIHAEEGDDRFADLCSVGSPFARASLKSGKNDSSSYFRRKEKMFRFFIRRMVKAQTFYWIVLCVVALNTLCVAMVHYDQPQRLTNALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPNTNFDTFPAAILTVFQILTGEDWNVVMYHGIESQGGVSKGMFSSFYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEEELEEAANQKLALQKAKEVAEVSPLSAANISIAAKQQNSAKARSVWEQRTSQIRLHNFQASCEALYSEMAPEERLRYTTTLHIRPDMKTHLDRPLVVEPRREEGVRAPGGKAGTGEGLEASEPTKVSPSAAAEGPEMPRKHHRHREKEKEKEKTGAGEQEKGEGVREENGESGVASKEERHRQRRSRSKETGGNKEGKCERSRGQEGGRRHHRRGSVEDVTAEKEHRRHRAHRHSAEPPGKEGNGTVSGAKPERRARHRGGSRSGTREGEPGPKGENGEEPPRRHKTRHKALSMYDSVEKEPGERDGEPPEKEMRNHQPKENQCEAEASGGMVTIPIHTLPSTCLQKVSEQPEDADNQKNVHRMTQPSLETLTIRIPVTLTAPGETTVVPMNNVDFEGKMEEKKDVEAGDVTRSGPRPILPYSSMFCLSPTNLLRRACHYIVNMRYFEMVILGVIALSSIALAAEDPVQADSPRNNVLKYMDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELQRDCRGQYLDYEKDEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTAVLMMKFYGAPYEYEMMLKGLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDETNINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSDRPCDPETKLKDECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALDIKLAPAGMKQHQCDAELRKEIASVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNTRDQGHQPPTGPVSLFHTQGQPAVLRGARVFLKQKSSTSLSNGGALPHPGSGIKESVSWGTQRTQDICYETRMPLERGHSAEIPVGQPSKQAVEMQEMAQDATSGEPQPGLESQGRAASMPRLAAEMQPIPDTSPMKRSISTLAPQRPHGSHLYEYGLERVSSGQPQHHHHHRCHRRRDKKQKSLDKCTSQSADGDGAPNHSVGMAAPSGEGPAPCKHERKQERGRSQERKQLSSSSSEKQRFYSCDRFGSRDPSQPKSSNGSRPTSPPGQEPGPHRQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFTGFQTSLPTFSPGRLSRGLSEHNTLLQRDSLSQPPTPVSRIGSDPYLGHRDHSDSSYRALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHHLRRVPNGYHCSLGLSSGPGTGGGRARQSYYHQTDEDDWC

Target ORF information:

RefSeq Version	XM_031951867.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951867.1

ORF Insert Sequence:

ATGGTCCGTT TCGGGGACGA GCTGCCCGGG CGCTATGGAG GAGCGGGCCC GGCGGGCGGC 
GGCCGGGGCG GCGGTGGGGC CGGGGCCGGG CCCGGGGGCC TGCAGCCCGG CCAACGCGTG 
CTTTACAAAC AGTCCATCGC GCAGCGGGCC CGCACTATGG CCCTGTACAA CCCCATCCCC 
GTCAAGCAGA ACTGCTTCAC AGTCAACCGT TCACTCTTCA TCTTCAGCGA GGACAACGTC 
GTCCGCAGAT ACGCCAAGCG CGTCACAGAA TGGCCTCCCT TTGAGTACAT GATCCTGGCC 
ACTATCATTG CCAATTGTAT CGTCTTAGCT CTGGAGCAAC ACCTTCCTGA TGGAGACAAG 
ACTCCCATGT CTGAGAGGCT GGATGACACT GAGCCCTATT TCATTGGGAT ATTTTGTTTT 
GAGGCTGGGA TCAAAATCAT CGCTTTGGGC TTCGTTTTCC ACAAGGGCTC ATATCTGAGG 
AACGGTTGGA ACGTGATGGA TTTCGTAGTA GTCCTCACAG GGATTCTGGC TACCGCTGGC 
ACTGAGTTTG ACCTCCGCAC CCTCCGAGCT GTGAGAGTGT TAAGACCATT GAAGCTGGTA 
TCAGGGATTC CAAGCCTCCA GGTTGTGCTC AAGTCCATCA TGAAGGCAAT GGTGCCCCTG 
CTGCAAATCG GCCTCCTGCT CTTCTTCGCC ATTGTCATGT TTGCCATCAT TGGACTTGAG 
TTTTATATGG GCAAGTTCCA CAAAGCCTGC TTCCCCAATA ACACAGCGGA CCCACACTCA 
GACCCTGTAG GAGACTTCCC CTGTGGCACT GATGCCTCAG CCCGACAGTG TGAGAATGGC 
TACATCTGCC GGGAATACTG GCGTGGTCCC AACTTTGGTA TTACCAACTT CGACAACATC 
CTTTTTGCGA TCCTGACCGT GTTCCAGTGT ATCACGATGG AAGGCTGGAC AGACATCCTC 
TACAACACTA ATGATGCAGC AGGAAATACC TGGAACTGGC TTTATTTCAT CCCTCTCATC 
ATCATTGGAT CTTTCTTCAT GCTCAACCTG GTTCTGGGAG TGCTCTCAGG GGAATTTGCC 
AAAGAGAGAG AGAGGGTAGA AAACCGTCGG GCTTTTCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAACGAG AGCTTAACGG TTACTTGGAG TGGATCTTCA AGGCTGAGGA GGTCATGTTG 
GCGGAAGAGG ACAAGAACGC AGATGAGAAG TCCCCTTTGG ATGTGCTGAA GAGAGCAGCC 
ACCAAGAAAA GTAAAAATGA TTTGATCCAT GCTGAAGAAG GGGACGATCG CTTCGCAGAC 
CTCTGCTCTG TGGGTTCTCC GTTTGCTAGA GCTAGTTTGA AGAGTGGGAA GAATGACAGC 
TCCTCCTATT TCCGGAGGAA AGAGAAGATG TTCCGCTTCT TCATCCGTCG CATGGTGAAA 
GCCCAGACCT TCTACTGGAT CGTCCTCTGT GTCGTGGCAT TGAACACACT GTGTGTGGCT 
ATGGTGCACT ATGACCAGCC ACAAAGACTC ACCAATGCTT TGTATTTTGC AGAATTTGTT 
TTCCTGGGTC TCTTCCTCAC TGAGATGTCT CTGAAGATGT ATGGCCTGGG GCCCAGAAAT 
TATTTCCATT CATCCTTCAA CTGCTTTGAC TTTGGGGTCA TCGTTGGAAG CATTTTTGAA 
GTCATCTGGG CAGCGATCAA GCCGGGGACT TCATTTGGAA TCAGTGTGCT GAGGGCTCTC 
CGGCTGCTGA GGATTTTCAA AGTCACAAAG TATTGGAATT CCCTTCGGAA CTTGGTGGTC 
TCCTTGCTGA ACTCCATGAA ATCCATCATC AGCCTGCTCT TCCTTCTCTT TCTGTTCATT 
GTGGTTTTCG CCCTGCTGGG GATGCAGCTC TTTGGAGGAC AGTTCAATTT TAGAGACGAG 
ACCCCGAACA CCAACTTTGA CACTTTTCCG GCTGCAATTC TCACTGTATT CCAGATCCTT 
ACTGGAGAAG ACTGGAACGT GGTCATGTAT CATGGGATAG AGTCCCAAGG GGGAGTCAGC 
AAAGGAATGT TCTCTTCTTT CTATTTTATC ATCCTGACAC TGTTCGGAAA CTACACCCTT 
CTGAATGTCT TCCTGGCTAT CGCTGTGGAC AATCTTGCCA ATGCCCAGGA ACTGACCAAG 
GATGAGGAAG AATTGGAGGA AGCAGCCAAT CAGAAGCTTG CTTTGCAGAA GGCCAAGGAG 
GTCGCTGAAG TCAGCCCCCT GTCTGCTGCC AACATCTCCA TAGCAGCCAA GCAGCAGAAC 
TCGGCCAAGG CCAGGTCTGT GTGGGAGCAG CGCACGAGTC AGATCCGGCT GCACAATTTC 
CAGGCCAGCT GCGAGGCCCT GTACAGCGAG ATGGCCCCGG AGGAGCGCCT CCGCTACACC 
ACCACCCTCC ACATCCGTCC CGACATGAAG ACGCACCTGG ACAGACCCCT GGTGGTGGAG 
CCCCGCAGAG AAGAAGGGGT CCGGGCCCCG GGAGGCAAAG CGGGGACCGG GGAGGGGCTG 
GAGGCCTCGG AGCCGACCAA GGTGTCCCCC AGCGCGGCTG CGGAGGGCCC CGAGATGCCC 
CGCAAGCACC ACCGGCACAG GGAGAAGGAG AAGGAGAAGG AAAAGACAGG GGCTGGGGAG 
CAGGAGAAGG GAGAAGGTGT CAGGGAGGAA AATGGGGAGT CGGGGGTCGC CAGTAAGGAG 
GAGCGGCACC GGCAGCGCCG GAGCAGAAGC AAGGAGACTG GGGGCAACAA AGAGGGGAAA 
TGCGAACGTT CCCGGGGCCA GGAGGGCGGC CGGAGGCACC ACCGCCGGGG TTCCGTGGAG 
GACGTCACCG CCGAGAAGGA GCACCGGCGG CACCGGGCCC ACCGGCACTC AGCAGAGCCG 
CCGGGCAAAG AGGGCAACGG CACCGTCAGT GGGGCCAAGC CCGAGCGTCG GGCGCGCCAT 
CGGGGGGGCT CCCGCTCGGG GACCCGGGAA GGAGAGCCTG GGCCCAAGGG AGAGAATGGA 
GAGGAGCCTC CCCGGAGGCA TAAGACCCGG CACAAAGCCC TCTCCATGTA CGACTCGGTG 
GAAAAGGAAC CTGGAGAGAG GGACGGGGAG CCTCCGGAGA AAGAGATGCG GAATCACCAG 
CCCAAGGAGA ACCAATGTGA GGCGGAGGCC AGTGGTGGGA TGGTGACCAT CCCCATTCAC 
ACACTGCCCA GTACCTGTCT GCAGAAGGTG AGCGAGCAGC CGGAAGACGC AGATAACCAG 
AAGAATGTGC ATCGCATGAC CCAACCATCC CTGGAGACCC TCACCATACG CATCCCAGTG 
ACCCTGACTG CCCCTGGAGA GACCACAGTG GTTCCAATGA ACAACGTTGA CTTTGAAGGT 
AAAATGGAAG AGAAGAAGGA CGTAGAAGCT GGTGACGTGA CAAGAAGTGG ACCCCGTCCA 
ATTCTCCCCT ATAGCTCCAT GTTCTGCTTA AGCCCCACCA ACCTACTCCG GCGTGCCTGC 
CACTACATAG TGAATATGCG TTACTTTGAA ATGGTCATCC TCGGGGTCAT CGCCCTCAGC 
AGCATTGCTC TGGCTGCTGA GGATCCAGTA CAGGCCGACT CGCCCAGGAA CAACGTTCTC 
AAGTACATGG ATTACATTTT CACTGGGGTC TTCACCTTTG AGATGGTAAT CAAGATGATT 
GACCTGGGGC TGCTGCTTCA TCCTGGCTCT TATTTCCGAG ACCTGTGGAA CATTCTGGAC 
TTCATTGTGG TCAGTGGGGC CCTGGTGGCT TTTGCCTTCT CGAGCTTTAT GGGAGGAAAA 
GGGAAAGACA TCAGCACAAT CAAGTCACTG CGAGTACTCC GAGTCCTGAG ACCTCTGAAG 
ACCATCAAAC GTTTGCCAAA ACTAAAGGCT GTGTTTGACT GTGTGGTAAA CTCCCTGAAG 
AATGTCCTCA ATATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC CGTCATTGCT 
GTGCAGCTCT TCAAAGGAAA ATTCTTCTAT TGCACAGATG AATCCAAAGA GCTTCAGAGG 
GATTGCAGGG GTCAGTATTT GGATTATGAA AAGGACGAAG TGGAGGCTCA ACCCAGACAA 
TGGAAGAAAT ATGACTTTCA CTATGACAAT GTGCTTTGGG CTTTGCTGAC TCTTTTCACA 
GTGTCTACAG GAGAAGGGTG GCCAACGGTA CTGAAGCATT CGGTGGATGC AACATATGAA 
GAACAGGGTC CAAGTCCTGG CTTCCGGATG GAACTGTCAA TCTTCTACGT GGTCTACTTT 
GTTGTGTTTC CCTTTTTCTT CGTCAATATC TTTGTGGCAT TGATCATCAT CACCTTCCAG 
GAGCAGGGAG ACAAAGTGAT GTCAGAATGC AGCTTGGAGA AGAATGAGAG GGCTTGCATA 
GACTTTGCCA TCAGTGCCAA GCCCCTGACC CGTTACATGC CTCAGAACAA GCAGTCCTTC 
CAGTACAAGA CATGGACCTT TGTGGTGTCT CCACCCTTTG AGTATTTCAT CATGGCCATG 
ATCGCCCTCA ACACGGCCGT CTTGATGATG AAGTTCTATG GTGCCCCCTA TGAGTATGAA 
ATGATGCTGA AGGGCTTGAA TATTGTATTC ACTTCAATGT TCTCTATGGA GTGTGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAATTAT TTCAGAGATG CCTGGAATGT TTTTGATTTT 
GTAACAGTAT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGAA CAACTTCATC 
AATCTCAGCT TCTTGCGGCT CTTTCGGGCA GCCCGACTCA TTAAACTGCT TCGCCAGGGA 
TATACTATCC GAATTCTCCT GTGGACCTTT GTTCAGTCCT TCAAGGCTCT GCCGTATGTC 
TGCTTACTCA TCGCCATGCT CTTCTTCATC TACGCCATCA TTGGCATGCA GGTCTTTGGA 
AACATTGCTC TTGATGATGA AACGAATATC AACCGGCACA ACAATTTCCG GACATTCCTG 
CAAGCCCTGA TGCTGCTGTT CAGGAGTGCT ACAGGGGAAG CCTGGCATGA GATCATGTTG 
TCATGTCTTA GTGACCGTCC CTGTGATCCT GAAACCAAAC TAAAAGATGA ATGTGGGAGT 
GACTTTGCTT ACTTTTACTT CGTCTCCTTC ATTTTCCTCT GCTCCTTTCT GATGCTGAAT 
CTCTTTGTGG CAGTGATTAT GGATAATTTT GAGTACCTGA CTCGAGACTC TTCCATCCTA 
GGACCTCATC ACCTGGACGA ATTTATTCGG GTCTGGGCTG AATATGACCC AGCTGCATGT 
GGGCGCATCA GTTACAGTGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC ACCCCTCGGC 
CTGGGAAAGA AATGCCCTGC TCGAGTTGCC TATAAGCGCC TGGTGCGAAT GAACATGCCC 
ATCTCCAATG AGGACATGAC TGTGCACTTC ACTTCCACCT TGATGGCCCT CATCCGGACG 
GCTCTGGACA TCAAGCTTGC ACCAGCGGGG ATGAAACAGC ATCAGTGTGA TGCAGAACTG 
AGGAAAGAGA TTGCATCTGT TTGGGCTAAT CTGCCCCAGA AGACACTGGA CTTACTGGTG 
CCTCCACATA AACCTGATGA GATGACTGTA GGAAAAGTCT ACGCAGCTCT GATGATTTTT 
GACTTCTACA AGCAGAACAA AAACACCCGA GATCAAGGTC ACCAGCCTCC GACAGGTCCT 
GTGTCTCTGT TCCATACCCA AGGCCAACCA GCTGTGCTCC GAGGTGCTAG AGTGTTCTTG 
AAACAGAAAA GTTCAACCTC CCTAAGCAAT GGTGGAGCCT TACCACACCC AGGGAGTGGT 
ATCAAGGAGT CTGTCTCATG GGGCACCCAG CGCACCCAGG ATATCTGCTA TGAGACCAGG 
ATGCCCCTGG AACGTGGTCA TTCTGCAGAA ATTCCTGTGG GACAACCTAG CAAACAAGCT 
GTGGAGATGC AAGAAATGGC TCAGGATGCC ACCAGTGGAG AACCCCAGCC AGGGCTGGAA 
AGTCAGGGCC GAGCGGCCTC CATGCCCCGC CTGGCTGCAG AGATGCAGCC CATTCCAGAC 
ACAAGCCCCA TGAAGCGTTC CATCTCCACC TTGGCCCCAC AGCGCCCACA TGGCTCACAC 
CTTTATGAAT ATGGTCTGGA ACGCGTATCC TCAGGCCAGC CTCAGCATCA TCACCATCAT 
CGCTGTCACC GGCGCAGGGA TAAGAAGCAG AAGTCTTTGG ACAAATGCAC TAGTCAGTCA 
GCAGATGGAG ATGGGGCACC AAACCATAGT GTGGGGATGG CAGCTCCATC AGGGGAAGGT 
CCAGCCCCAT GTAAGCATGA GAGGAAGCAA GAAAGAGGGC GATCCCAGGA GCGGAAGCAG 
CTCTCTTCCT CCTCTTCAGA GAAGCAGCGT TTCTATTCTT GTGACCGATT TGGGAGCCGG 
GACCCATCAC AGCCCAAATC CTCCAATGGA AGTCGTCCTA CATCACCTCC TGGGCAGGAG 
CCAGGTCCCC ACAGACAGGG TAGTGGTTCA GTTAATGGGA GCCCCTTGCT GTCAACATCT 
GGTGCTAGTA CACCGGGCCG TGGTGGGCGG AGGCAGCTCC CCCAGACACC TCTGACCCCC 
CGTCCCAGCA TCACATACAA GACTGCGAAC TCCTCGCCCG TCCACTTCAC CGGTTTCCAG 
ACCAGCCTGC CCACCTTCTC CCCGGGACGG CTCAGTCGAG GCCTTTCTGA GCACAACACC 
CTGCTCCAGA GGGATTCTCT GAGCCAGCCG CCAACCCCAG TGTCTCGGAT TGGCTCGGAC 
CCATACCTGG GGCACCGTGA CCACAGCGAT TCCTCGTACC GGGCCCTGCC CGAGGACACA 
CTCACTTTTG AGGAAGCCGT CGCCACCAAC TCGGGCCGCT CATCGAGGAC TTCCTACGTG 
TCCTCTCTAA CATCCCAGTC CCACCATCTC CGTCGTGTGC CCAATGGTTA CCACTGCAGT 
CTGGGGCTCA GCTCCGGCCC CGGGACTGGC GGTGGCAGGG CCCGGCAGAG TTATTATCAC 
CAAACCGACG AAGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CACNA1B
Entrez Gene ID	100933192
Full Name	calcium voltage-gated channel subunit alpha1 B
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CACNA1B cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

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