MAP3K4 gene cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

mRNA	Protein	Name
XM_031937526.1	XP_031793386.1	mitogen-activated protein kinase kinase kinase 4 isoform X6
XM_031937528.1	XP_031793388.1	mitogen-activated protein kinase kinase kinase 4 isoform X8
XM_031937527.1	XP_031793387.1	mitogen-activated protein kinase kinase kinase 4 isoform X7
XM_023503320.1	XP_023359088.1	mitogen-activated protein kinase kinase kinase 4
XM_012549451.1	XP_012404905.1	mitogen-activated protein kinase kinase kinase 4
XM_031937525.1	XP_031793385.1	mitogen-activated protein kinase kinase kinase 4 isoform X5
XM_031937524.1	XP_031793384.1	mitogen-activated protein kinase kinase kinase 4 isoform X4
XM_031937521.1	XP_031793381.1	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_031937522.1	XP_031793382.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_031937523.1	XP_031793383.1	mitogen-activated protein kinase kinase kinase 4 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa236414	XM_031937528.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa131785	XM_012549451.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa155063	XM_023503320.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa236407	XM_031937521.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa236408	XM_031937522.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa236409	XM_031937523.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa236410	XM_031937524.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa236411	XM_031937525.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa236412	XM_031937526.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa236413	XM_031937527.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

MAP3K4 ( XM_031937528.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031793388.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OSa236414
Clone ID Related Accession (Same CDS sequence)	XM_031937528.1
Accession Version	XM_031937528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCAGCTATA 
CATCGGAACA GCCCCCGTCC TGTGAAGGTA CCCCGATGTC ACAGTGACCC TCCCAACCCA 
CATCTCATTA TCCCAACTCC TGATGGATTC AGCACTCGGA GTGTGCCTTC TGACGTGCGG 
AACCCATGCA GTGCTGCTGC CAGTCGCCCC GCCCCAGCCG GCAGCGACTC CGCTCCACTC 
AAACCTGTCA GCAATACAAA TGATAACAGG GGATCCAGTG TTCCAGAAAA TGATCGTTTG 
GCCTCGATAG CTGCTGAATT GCAATTCAGA TCATTGAGTC GACATTCAAG CCCCACTGAG 
GAGCGAGAAG AGCCTTCATA CCCAAAAGGA GATTCAAGTG GGCCAACACG AAGAAGTTGG 
GAGCTTCGGA CTCTGATCAG CCAAAATAAA GACACCGCTT CTAAACAAGG ACCCATAGAA 
GCTGTTCAAA AATCAGTTCG ATTGTTTGAA GAAAAACGTT ATCGAGAAAT GAGGAGAAAG 
AATATCATTG GTCAAGTTTG TGACACCCCC AAATCATATG ATAATGTCAT GCATGTTGGA 
TTACGAAAAG TGACCTTCAA ATGGCAAAGA GGAAATAAAA TTGGAGAAGG CCAGTATGGA 
AAAGTCTATA CTTGTATCAG TGTTGATACT GGTGAATTGA TGGCAATGAA GGAAATTCGA 
TTTCAGCCGA ATGACCACAA AACTATCAAA GAGACTGCAG ATGAACTGAA AATATTTGAA 
GGCATTAAGC ACCCCAATCT GGTTCGGTAT TTTGGTGTGG AGCTTCATAG AAGCACAGTA 
ACTTTGCCTA ATTATAAGGA AATCTCCATG AGTTACATCA TTTCTGCCTT CTCTCTGAAA 
CTAGAGGAAT TCTTCCTAAA ATGTAAGTTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031793388.1
CDS	307..4599
Translation	MREAVAAPELVSPPAGAAAAAANAMEGPPPPPPELPLQLEGCQAARQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFSTRSVPSDVRNPCSAAASRPAPAGSDSAPLKPVSNTNDNRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHRSTVTLPNYKEISMSYIISAFSLKLEEFFLKCKL

Target ORF information:

RefSeq Version	XM_031937528.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031937528.1

ORF Insert Sequence:

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCAGCTATA 
CATCGGAACA GCCCCCGTCC TGTGAAGGTA CCCCGATGTC ACAGTGACCC TCCCAACCCA 
CATCTCATTA TCCCAACTCC TGATGGATTC AGCACTCGGA GTGTGCCTTC TGACGTGCGG 
AACCCATGCA GTGCTGCTGC CAGTCGCCCC GCCCCAGCCG GCAGCGACTC CGCTCCACTC 
AAACCTGTCA GCAATACAAA TGATAACAGG GGATCCAGTG TTCCAGAAAA TGATCGTTTG 
GCCTCGATAG CTGCTGAATT GCAATTCAGA TCATTGAGTC GACATTCAAG CCCCACTGAG 
GAGCGAGAAG AGCCTTCATA CCCAAAAGGA GATTCAAGTG GGCCAACACG AAGAAGTTGG 
GAGCTTCGGA CTCTGATCAG CCAAAATAAA GACACCGCTT CTAAACAAGG ACCCATAGAA 
GCTGTTCAAA AATCAGTTCG ATTGTTTGAA GAAAAACGTT ATCGAGAAAT GAGGAGAAAG 
AATATCATTG GTCAAGTTTG TGACACCCCC AAATCATATG ATAATGTCAT GCATGTTGGA 
TTACGAAAAG TGACCTTCAA ATGGCAAAGA GGAAATAAAA TTGGAGAAGG CCAGTATGGA 
AAAGTCTATA CTTGTATCAG TGTTGATACT GGTGAATTGA TGGCAATGAA GGAAATTCGA 
TTTCAGCCGA ATGACCACAA AACTATCAAA GAGACTGCAG ATGAACTGAA AATATTTGAA 
GGCATTAAGC ACCCCAATCT GGTTCGGTAT TTTGGTGTGG AGCTTCATAG AAGCACAGTA 
ACTTTGCCTA ATTATAAGGA AATCTCCATG AGTTACATCA TTTCTGCCTT CTCTCTGAAA 
CTAGAGGAAT TCTTCCTAAA ATGTAAGTTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_012549451.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012404905.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), mRNA.

Price & Availability↑

CloneID	OSa131785
Clone ID Related Accession (Same CDS sequence)	XM_012549451.1
Accession Version	XM_012549451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGCGGGGAG CTGGGGGTCG CCCCTCCGCT CAGGTGCTGC AGGTCCGCCG GGCTCGGCGC 
AGCCTGCTCC TCAGCCCCGC ATTCTTAGCA CCGCCGGGCG TGCAGGAGTC CGGTCCTGGG 
CAGGAATGCA AGTTGGGAGA TGTTGCTTGC AAAAACTCTG AATCTGATCC AGAGGAGTTC 
TCAGATGAAA CAAATACTGA AAACCTTTAT GGTGCCTCTC CTCCCAGCAC ACCTCGACAG 
ATGAAACGTA TGTCAACAAA ACACCAGAGG AATAATGTAG GGAGACCTTC TGGCCGCTCC 
AGTTTAAAAG AAAAAATGAA TGCACCAAAT CAGCTTCCTC ACAAAGACAT TTGCAAAACA 
GTAGAGAATG TGGAAGAACA CAGTTATAAG CAAGAAAAAA AGATCCGAGC CACTCTTAGA 
ACAACAGAAC GGGATCATAA AAAAAATGTA CAGTGTTCAT TCATGTTAGA CGCAGTGGGT 
GGGTCTTTGC CAAAAAAGCC AATTCCGGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT 
GGCTGTAGCA ATGCTAAGCT TCCGGTTTCT ATGCCTATAC CTATAGCCAG AACTGCACGC 
CAGACTTCCA GGACTGATTG TCCTTTGGAA CGCTTAAAAT TCTTTGAAAC TTTACGACTT 
CTGCTAAAGC TTACCTCAGT CTCAAAGAAA AAAGACAGGG AGCAGAGAGG GCAGGAAAAC 
ACTTCTGCTT TCTGGTTTAA CCGATCCAAT GAACTCATTT GGCTGGAATT ACAAGCCTGG 
CATGCCGGAC GGACCATTAA TGACCAGGAC CTTTTTTTAT ATGCTGCCCG TCAAGCCATC 
CCCGATATTA TCAATGAAAT CCTCACATTC AAAGTAGATT ATGGGAGTTT TGCCTGTGTG 
AGAAATGGAG CTAGTTTTAA TGGTACTTCA GTGGAAGGAC AGAGCAGAGC ACCCCCAGGA 
GCCAATGCTG TGGGTTATTC AAACTATCAC GAACATCTCC AACGCCAGCG GGTCTCCTTT 
GAACAGGTAA AGCGGATAAT GGAGCAGCTT GAATATATAG AAGCACTTTA TCCCTCTTTG 
CAGGCTCTTC AAAAGGACTA TGAAAAATAT GCTGCAAAAG ATTTCCAAGA CAGGGTGCAG 
GCACTCTGTC TGTGGCTAAA CATTACGAAA GACTTAAATC AGAAACTAAG GATTATGGGA 
ACTGTTTTGG GCATCAAAAA TTTATCTGAC ATTGGCTGGC CAGTGTTTGA AATACCGTCT 
CCCCGACCTT CCAAAGGAAA CGAGCCGGAG GAGGAGGGGA GTGACACAGA AGGAGAAGTG 
AAGGAATTGG AAACTACCAC CGATGAAAGT GAAGAAGAAC ATGTTCTAGA TCAGGGTGTC 
AAGGACTTCA GGCGGGCCAT GGAGAGCAGT TTGGACCTCC AGCCCCAGGA CCATTTTTCA 
AGGAAGTTTG ACAGACTGGA GTCTGAAGAT GAATCTATTG GCTGGGGAGC GTCCGAGTAC 
AGTGCTGAAA CAGGCTATAG TAGATACTGT CTGACTTCTA TATATAGACC ATTTGTAGAC 
AAAGCTCTCA AACAGATGGG TTTGAGAAAA TTAATTGTAA GACTTCACAA GCTAATGGAT 
GGTTCTTTAC AAAGGGCCCG TATAGCACTG GTTAAGAGTG ATCATCCATT GGAGTTTTCT 
GAGTTCCCAG ATCCTATGTT GTGTTCAGAT TATATACAGT TGTCAAATAC AACACCGTCA 
TCAGAACAAA AGTGCAGCAC AGTGTCATGG GAAGAGCTGA AATCTATGGA TTTGCCATCA 
TTTGAGCCTG CATTTTTGGT CCTTTGTCGA GTGCTTCTTA ATGTTATCCA TGAGTGTCTC 
AAATTAAGAT TGGAACAGAG ACCTGCTGGG GAACCATCTC TCTTGAGTAT TAAGCAGCTG 
GTAAGAGAGT GTAAAGAGGT TCTGAAGGGT GGCCTGTTAA TGAAGCAATA TTATCAATTT 
ATGCTTCATG AAGTATTAGA TGATTTGGAA AAGATTGACT GCAATATAGA CTCTTTTGAA 
GAGGACCTGC ACAAAATGTT GATGGTGTAT TTTGATTACA TGAGAAGTTG GATCAAAATG 
TTACAGCAGT TACCTCAAGC ATCTCATAGT TTAAAAAATC TATTAGAAGA AGAATGGAAT 
TTCACCAAAG AAATAACTCA TTACATAAGA GGAGGAGAAG CACAGGCTGG AAAATTGTTT 
TGTGACATTG CAGGAATGCT GCTGAAATCT ACTGGAGGAT TTTTAGATTG TGGCCTGCAG 
GAAAGCTGTG CTGAATTCTG GACTAGTGCT GATGATAGCA CTGCTTCAGA TGAAATCAGG 
AGATCTGTTA TAGAGACCAG CAGAGCCCTG AAGGAGCTCT TTCATGAAGC TAGGGAAAGG 
GCTTCCAAAG CACTTGGCTT TGCTAAAATG CTAAGAAAGG ATCTGGAAAT AGCAGCAGAA 
TTCATAGTAT CAGCACCAGT ACAAGACCTT CTGGATATGT TGAAATCAAA ACAATATGTT 
AAGGTGCAAA TTCCTGGATT GGAAAATTTG CAGTTATTTG TTCCACATAC TCTTGCTGGG 
GAGAAATGTG TTATTTTGCA ACTACTGAAT GCAGCTGCAG GAAAGGACTG TTCGAAAGAT 
TCAGATGATG TAGTAATTGA AGCTTACTTA CTGATGACCA AGCATGGTGA TCGAGATCCT 
GAGTTAGATG ACCACTGGAG TACATGGGAA GGGACGCCTG TAAAAATAGT GCCTCAGGTG 
GAAACAGTTG ACACACTTAG AAGTATGCAG GTTGAAAATC TACTCTTGGT AGTCATGCAA 
TCTGCTCACC TTGTGATCCA GAGAAAAGCT TTCCAGCAAT CCATTGAAGG GCTTGTAACT 
TTGCACCAAG AACAGACTTC GAGTCAGCCA ATTATTGCCA AAGCTTTGCA GCAGCTAAAG 
GATGATGCAT TGCAGCTGTG CAACAAAATA AGTGATGCCA TTGACCAAGT AGACCACATA 
TTCACATCAG AATTTGATAC TGAAGTTGAT GAGTCAGAGT CTGCCACCTT GCAGCAGTAC 
TATCGTGAAG CCATGATTCA AGGTTACAAT TTTGGTTTTG AGTATCACAA AGAAGTTGTT 
CGTCTGATGT CTGGAACATT TAGACAGAAG ATTGGAGATA AATATATTAG CTTTGCCAGA 
AAGTGGATGA ATTATGTGCT AACTAAATGT GAGAGTGGTC GTGGCACCAG ACCCAGGTGG 
GCAACTCAAG GTTTTGATTT TCTGCAAGCA ATTGAACCAG GCTTTATTTC AGCACTTCCT 
GAAGATGACT TTTTGAGTTT ACAAGCTTTG ATGAATGAAT GCATTGGTCA TGTGATAGGA 
AAACCTCACA GTCCTGTTAC AGCTATACAT CGGAACAGCC CCCGTCCTGT GAAGGTACCC 
CGATGTCACA GTGACCCTCC CAACCCACAT CTCATTATCC CAACTCCTGA TGGATTCAGG 
GGATCCAGTG TTCCAGAAAA TGATCGTTTG GCCTCGATAG CTGCTGAATT GCAATTCAGA 
TCATTGAGTC GACATTCAAG CCCCACTGAG GAGCGAGAAG AGCCTTCATA CCCAAAAGGA 
GATTCAAGTG GGCCAACACG AAGAAGTTGG GAGCTTCGGA CTCTGATCAG CCAAAATAAA 
GACACCGCTT CTAAACAAGG ACCCATAGAA GCTGTTCAAA AATCAGTTCG ATTGTTTGAA 
GAAAAACGTT ATCGAGAAAT GAGGAGAAAG AATATCATTG GTCAAGTTTG TGACACCCCC 
AAATCATATG ATAATGTCAT GCATGTTGGA TTACGAAAAG TGACCTTCAA ATGGCAAAGA 
GGAAATAAAA TTGGAGAAGG CCAGTATGGA AAAGTCTATA CTTGTATCAG TGTTGATACT 
GGTGAATTGA TGGCAATGAA GGAAATTCGA TTTCAGCCGA ATGACCACAA AACTATCAAA 
GAGACTGCAG ATGAACTGAA AATATTTGAA GGCATTAAGC ACCCCAATCT GGTTCGGTAT 
TTTGGTGTGG AGCTTCATAG AGAAGAAATG TATATTTTCA TGGAATATTG TGATGAGGGC 
ACCCTGGAAG AAGTGTCAAG GCTGGGCCTT CAAGAACATG TTATTCGCTT GTACTCAAAG 
CAGATCACCA TTGCTATTAA TGTACTGCAT GAGCATGGTA TAGTTCATCG TGACATCAAA 
GGAGCCAATA TCTTCCTTAC ATCATCTGGA CTAATTAAAT TGGGGGATTT TGGATGCTCA 
GTCAAGTTAA AAAACAATGC ACAAACCATG CCTGGAGAAG TGAACAGTAC TCTCGGGACT 
GCAGCGTACA TGGCACCTGA AGTCATCACT CGAGCGAAAG GAGAAGGACA TGGGCGTGCA 
GCAGACATCT GGAGTTTGGG TTGTGTTGTG ATAGAAATGG TTACTGGCAA GAGGCCTTGG 
CATGAGTATG AGCACAACTT TCAGATCATG TATAAGGTTG GAATGGGACA TAAGCCACCC 
ATCCCTGAAA GGATAAGCCC TGAAGGAAAA GATTTCCTTT CCCATTGCCT CGAAAGTGAT 
CCAAAGATGA GATGGACAGC TAGCCAGCTC CTCGACCATT CGTTTGTCAA GGTTTGCACA 
GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012404905.1
CDS	1..4632
Translation	MRGAGGRPSAQVLQVRRARRSLLLSPAFLAPPGVQESGPGQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERISPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_012549451.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012549451.1

ORF Insert Sequence:

ATGCGGGGAG CTGGGGGTCG CCCCTCCGCT CAGGTGCTGC AGGTCCGCCG GGCTCGGCGC 
AGCCTGCTCC TCAGCCCCGC ATTCTTAGCA CCGCCGGGCG TGCAGGAGTC CGGTCCTGGG 
CAGGAATGCA AGTTGGGAGA TGTTGCTTGC AAAAACTCTG AATCTGATCC AGAGGAGTTC 
TCAGATGAAA CAAATACTGA AAACCTTTAT GGTGCCTCTC CTCCCAGCAC ACCTCGACAG 
ATGAAACGTA TGTCAACAAA ACACCAGAGG AATAATGTAG GGAGACCTTC TGGCCGCTCC 
AGTTTAAAAG AAAAAATGAA TGCACCAAAT CAGCTTCCTC ACAAAGACAT TTGCAAAACA 
GTAGAGAATG TGGAAGAACA CAGTTATAAG CAAGAAAAAA AGATCCGAGC CACTCTTAGA 
ACAACAGAAC GGGATCATAA AAAAAATGTA CAGTGTTCAT TCATGTTAGA CGCAGTGGGT 
GGGTCTTTGC CAAAAAAGCC AATTCCGGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT 
GGCTGTAGCA ATGCTAAGCT TCCGGTTTCT ATGCCTATAC CTATAGCCAG AACTGCACGC 
CAGACTTCCA GGACTGATTG TCCTTTGGAA CGCTTAAAAT TCTTTGAAAC TTTACGACTT 
CTGCTAAAGC TTACCTCAGT CTCAAAGAAA AAAGACAGGG AGCAGAGAGG GCAGGAAAAC 
ACTTCTGCTT TCTGGTTTAA CCGATCCAAT GAACTCATTT GGCTGGAATT ACAAGCCTGG 
CATGCCGGAC GGACCATTAA TGACCAGGAC CTTTTTTTAT ATGCTGCCCG TCAAGCCATC 
CCCGATATTA TCAATGAAAT CCTCACATTC AAAGTAGATT ATGGGAGTTT TGCCTGTGTG 
AGAAATGGAG CTAGTTTTAA TGGTACTTCA GTGGAAGGAC AGAGCAGAGC ACCCCCAGGA 
GCCAATGCTG TGGGTTATTC AAACTATCAC GAACATCTCC AACGCCAGCG GGTCTCCTTT 
GAACAGGTAA AGCGGATAAT GGAGCAGCTT GAATATATAG AAGCACTTTA TCCCTCTTTG 
CAGGCTCTTC AAAAGGACTA TGAAAAATAT GCTGCAAAAG ATTTCCAAGA CAGGGTGCAG 
GCACTCTGTC TGTGGCTAAA CATTACGAAA GACTTAAATC AGAAACTAAG GATTATGGGA 
ACTGTTTTGG GCATCAAAAA TTTATCTGAC ATTGGCTGGC CAGTGTTTGA AATACCGTCT 
CCCCGACCTT CCAAAGGAAA CGAGCCGGAG GAGGAGGGGA GTGACACAGA AGGAGAAGTG 
AAGGAATTGG AAACTACCAC CGATGAAAGT GAAGAAGAAC ATGTTCTAGA TCAGGGTGTC 
AAGGACTTCA GGCGGGCCAT GGAGAGCAGT TTGGACCTCC AGCCCCAGGA CCATTTTTCA 
AGGAAGTTTG ACAGACTGGA GTCTGAAGAT GAATCTATTG GCTGGGGAGC GTCCGAGTAC 
AGTGCTGAAA CAGGCTATAG TAGATACTGT CTGACTTCTA TATATAGACC ATTTGTAGAC 
AAAGCTCTCA AACAGATGGG TTTGAGAAAA TTAATTGTAA GACTTCACAA GCTAATGGAT 
GGTTCTTTAC AAAGGGCCCG TATAGCACTG GTTAAGAGTG ATCATCCATT GGAGTTTTCT 
GAGTTCCCAG ATCCTATGTT GTGTTCAGAT TATATACAGT TGTCAAATAC AACACCGTCA 
TCAGAACAAA AGTGCAGCAC AGTGTCATGG GAAGAGCTGA AATCTATGGA TTTGCCATCA 
TTTGAGCCTG CATTTTTGGT CCTTTGTCGA GTGCTTCTTA ATGTTATCCA TGAGTGTCTC 
AAATTAAGAT TGGAACAGAG ACCTGCTGGG GAACCATCTC TCTTGAGTAT TAAGCAGCTG 
GTAAGAGAGT GTAAAGAGGT TCTGAAGGGT GGCCTGTTAA TGAAGCAATA TTATCAATTT 
ATGCTTCATG AAGTATTAGA TGATTTGGAA AAGATTGACT GCAATATAGA CTCTTTTGAA 
GAGGACCTGC ACAAAATGTT GATGGTGTAT TTTGATTACA TGAGAAGTTG GATCAAAATG 
TTACAGCAGT TACCTCAAGC ATCTCATAGT TTAAAAAATC TATTAGAAGA AGAATGGAAT 
TTCACCAAAG AAATAACTCA TTACATAAGA GGAGGAGAAG CACAGGCTGG AAAATTGTTT 
TGTGACATTG CAGGAATGCT GCTGAAATCT ACTGGAGGAT TTTTAGATTG TGGCCTGCAG 
GAAAGCTGTG CTGAATTCTG GACTAGTGCT GATGATAGCA CTGCTTCAGA TGAAATCAGG 
AGATCTGTTA TAGAGACCAG CAGAGCCCTG AAGGAGCTCT TTCATGAAGC TAGGGAAAGG 
GCTTCCAAAG CACTTGGCTT TGCTAAAATG CTAAGAAAGG ATCTGGAAAT AGCAGCAGAA 
TTCATAGTAT CAGCACCAGT ACAAGACCTT CTGGATATGT TGAAATCAAA ACAATATGTT 
AAGGTGCAAA TTCCTGGATT GGAAAATTTG CAGTTATTTG TTCCACATAC TCTTGCTGGG 
GAGAAATGTG TTATTTTGCA ACTACTGAAT GCAGCTGCAG GAAAGGACTG TTCGAAAGAT 
TCAGATGATG TAGTAATTGA AGCTTACTTA CTGATGACCA AGCATGGTGA TCGAGATCCT 
GAGTTAGATG ACCACTGGAG TACATGGGAA GGGACGCCTG TAAAAATAGT GCCTCAGGTG 
GAAACAGTTG ACACACTTAG AAGTATGCAG GTTGAAAATC TACTCTTGGT AGTCATGCAA 
TCTGCTCACC TTGTGATCCA GAGAAAAGCT TTCCAGCAAT CCATTGAAGG GCTTGTAACT 
TTGCACCAAG AACAGACTTC GAGTCAGCCA ATTATTGCCA AAGCTTTGCA GCAGCTAAAG 
GATGATGCAT TGCAGCTGTG CAACAAAATA AGTGATGCCA TTGACCAAGT AGACCACATA 
TTCACATCAG AATTTGATAC TGAAGTTGAT GAGTCAGAGT CTGCCACCTT GCAGCAGTAC 
TATCGTGAAG CCATGATTCA AGGTTACAAT TTTGGTTTTG AGTATCACAA AGAAGTTGTT 
CGTCTGATGT CTGGAACATT TAGACAGAAG ATTGGAGATA AATATATTAG CTTTGCCAGA 
AAGTGGATGA ATTATGTGCT AACTAAATGT GAGAGTGGTC GTGGCACCAG ACCCAGGTGG 
GCAACTCAAG GTTTTGATTT TCTGCAAGCA ATTGAACCAG GCTTTATTTC AGCACTTCCT 
GAAGATGACT TTTTGAGTTT ACAAGCTTTG ATGAATGAAT GCATTGGTCA TGTGATAGGA 
AAACCTCACA GTCCTGTTAC AGCTATACAT CGGAACAGCC CCCGTCCTGT GAAGGTACCC 
CGATGTCACA GTGACCCTCC CAACCCACAT CTCATTATCC CAACTCCTGA TGGATTCAGG 
GGATCCAGTG TTCCAGAAAA TGATCGTTTG GCCTCGATAG CTGCTGAATT GCAATTCAGA 
TCATTGAGTC GACATTCAAG CCCCACTGAG GAGCGAGAAG AGCCTTCATA CCCAAAAGGA 
GATTCAAGTG GGCCAACACG AAGAAGTTGG GAGCTTCGGA CTCTGATCAG CCAAAATAAA 
GACACCGCTT CTAAACAAGG ACCCATAGAA GCTGTTCAAA AATCAGTTCG ATTGTTTGAA 
GAAAAACGTT ATCGAGAAAT GAGGAGAAAG AATATCATTG GTCAAGTTTG TGACACCCCC 
AAATCATATG ATAATGTCAT GCATGTTGGA TTACGAAAAG TGACCTTCAA ATGGCAAAGA 
GGAAATAAAA TTGGAGAAGG CCAGTATGGA AAAGTCTATA CTTGTATCAG TGTTGATACT 
GGTGAATTGA TGGCAATGAA GGAAATTCGA TTTCAGCCGA ATGACCACAA AACTATCAAA 
GAGACTGCAG ATGAACTGAA AATATTTGAA GGCATTAAGC ACCCCAATCT GGTTCGGTAT 
TTTGGTGTGG AGCTTCATAG AGAAGAAATG TATATTTTCA TGGAATATTG TGATGAGGGC 
ACCCTGGAAG AAGTGTCAAG GCTGGGCCTT CAAGAACATG TTATTCGCTT GTACTCAAAG 
CAGATCACCA TTGCTATTAA TGTACTGCAT GAGCATGGTA TAGTTCATCG TGACATCAAA 
GGAGCCAATA TCTTCCTTAC ATCATCTGGA CTAATTAAAT TGGGGGATTT TGGATGCTCA 
GTCAAGTTAA AAAACAATGC ACAAACCATG CCTGGAGAAG TGAACAGTAC TCTCGGGACT 
GCAGCGTACA TGGCACCTGA AGTCATCACT CGAGCGAAAG GAGAAGGACA TGGGCGTGCA 
GCAGACATCT GGAGTTTGGG TTGTGTTGTG ATAGAAATGG TTACTGGCAA GAGGCCTTGG 
CATGAGTATG AGCACAACTT TCAGATCATG TATAAGGTTG GAATGGGACA TAAGCCACCC 
ATCCCTGAAA GGATAAGCCC TGAAGGAAAA GATTTCCTTT CCCATTGCCT CGAAAGTGAT 
CCAAAGATGA GATGGACAGC TAGCCAGCTC CTCGACCATT CGTTTGTCAA GGTTTGCACA 
GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_023503320.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023359088.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), mRNA.

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CloneID	OSa155063
Clone ID Related Accession (Same CDS sequence)	XM_023503320.1
Accession Version	XM_023503320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGCGGGGAG CTGGGGGTCG CCCCTCCGCT CAGGTGCTGC AGGTCCGCCG GGCTCGGCGC 
AGCCTGCTCC TCAGCCCCGC ATTCTTAGCA CCGCCGGGCG TGCAGGAGTC CGGTCCTGGG 
CAGGAATGCA AGTTGGGAGA TGTTGCTTGC AAAAACTCTG AATCTGATCC AGAGGAGTTC 
TCAGATGAAA CAAATACTGA AAACCTTTAT GGTGCCTCTC CTCCCAGCAC ACCTCGACAG 
ATGAAACGTA TGTCAACAAA ACACCAGAGG AATAATGTAG GGAGACCTTC TGGCCGCTCC 
AGTTTAAAAG AAAAAATGAA TGCACCAAAT CAGCTTCCTC ACAAAGACAT TTGCAAAACA 
GTAGAGAATG TGGAAGAACA CAGTTATAAG CAAGAAAAAA AGATCCGAGC CACTCTTAGA 
ACAACAGAAC GGGATCATAA AAAAAATGTA CAGTGTTCAT TCATGTTAGA CGCAGTGGGT 
GGGTCTTTGC CAAAAAAGCC AATTCCGGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT 
GGCTGTAGCA ATGCTAAGCT TCCGGTTTCT ATGCCTATAC CTATAGCCAG AACTGCACGC 
CAGACTTCCA GGACTGATTG TCCTTTGGAA CGCTTAAAAT TCTTTGAAAC TTTACGACTT 
CTGCTAAAGC TTACCTCAGT CTCAAAGAAA AAAGACAGGG AGCAGAGAGG GCAGGAAAAC 
ACTTCTGCTT TCTGGTTTAA CCGATCCAAT GAACTCATTT GGCTGGAATT ACAAGCCTGG 
CATGCCGGAC GGACCATTAA TGACCAGGAC CTTTTTTTAT ATGCTGCCCG TCAAGCCATC 
CCCGATATTA TCAATGAAAT CCTCACATTC AAAGTAGATT ATGGGAGTTT TGCCTGTGTG 
AGAAATGGAG CTAGTTTTAA TGGTACTTCA GTGGAAGGAC AGAGCAGAGC ACCCCCAGGA 
GCCAATGCTG TGGGTTATTC AAACTATCAC GAACATCTCC AACGCCAGCG GGTCTCCTTT 
GAACAGGTAA AGCGGATAAT GGAGCAGCTT GAATATATAG AAGCACTTTA TCCCTCTTTG 
CAGGCTCTTC AAAAGGACTA TGAAAAATAT GCTGCAAAAG ATTTCCAAGA CAGGGTGCAG 
GCACTCTGTC TGTGGCTAAA CATTACGAAA GACTTAAATC AGAAACTAAG GATTATGGGA 
ACTGTTTTGG GCATCAAAAA TTTATCTGAC ATTGGCTGGC CAGTGTTTGA AATACCGTCT 
CCCCGACCTT CCAAAGGAAA CGAGCCGGAG GAGGAGGGGA GTGACACAGA AGGAGAAGTG 
AAGGAATTGG AAACTACCAC CGATGAAAGT GAAGAAGAAC ATGTTCTAGA TCAGGGTGTC 
AAGGACTTCA GGCGGGCCAT GGAGAGCAGT TTGGACCTCC AGCCCCAGGA CCATTTTTCA 
AGGAAGTTTG ACAGACTGGA GTCTGAAGAT GAATCTATTG GCTGGGGAGC GTCCGAGTAC 
AGTGCTGAAA CAGGCTATAG TAGATACTGT CTGACTTCTA TATATAGACC ATTTGTAGAC 
AAAGCTCTCA AACAGATGGG TTTGAGAAAA TTAATTGTAA GACTTCACAA GCTAATGGAT 
GGTTCTTTAC AAAGGGCCCG TATAGCACTG GTTAAGAGTG ATCATCCATT GGAGTTTTCT 
GAGTTCCCAG ATCCTATGTT GTGTTCAGAT TATATACAGT TGTCAAATAC AACACCGTCA 
TCAGAACAAA AGTGCAGCAC AGTGTCATGG GAAGAGCTGA AATCTATGGA TTTGCCATCA 
TTTGAGCCTG CATTTTTGGT CCTTTGTCGA GTGCTTCTTA ATGTTATCCA TGAGTGTCTC 
AAATTAAGAT TGGAACAGAG ACCTGCTGGG GAACCATCTC TCTTGAGTAT TAAGCAGCTG 
GTAAGAGAGT GTAAAGAGGT TCTGAAGGGT GGCCTGTTAA TGAAGCAATA TTATCAATTT 
ATGCTTCATG AAGTATTAGA TGATTTGGAA AAGATTGACT GCAATATAGA CTCTTTTGAA 
GAGGACCTGC ACAAAATGTT GATGGTGTAT TTTGATTACA TGAGAAGTTG GATCAAAATG 
TTACAGCAGT TACCTCAAGC ATCTCATAGT TTAAAAAATC TATTAGAAGA AGAATGGAAT 
TTCACCAAAG AAATAACTCA TTACATAAGA GGAGGAGAAG CACAGGCTGG AAAATTGTTT 
TGTGACATTG CAGGAATGCT GCTGAAATCT ACTGGAGGAT TTTTAGATTG TGGCCTGCAG 
GAAAGCTGTG CTGAATTCTG GACTAGTGCT GATGATAGCA CTGCTTCAGA TGAAATCAGG 
AGATCTGTTA TAGAGACCAG CAGAGCCCTG AAGGAGCTCT TTCATGAAGC TAGGGAAAGG 
GCTTCCAAAG CACTTGGCTT TGCTAAAATG CTAAGAAAGG ATCTGGAAAT AGCAGCAGAA 
TTCATAGTAT CAGCACCAGT ACAAGACCTT CTGGATATGT TGAAATCAAA ACAATATGTT 
AAGGTGCAAA TTCCTGGATT GGAAAATTTG CAGTTATTTG TTCCACATAC TCTTGCTGGG 
GAGAAATGTG TTATTTTGCA ACTACTGAAT GCAGCTGCAG GAAAGGACTG TTCGAAAGAT 
TCAGATGATG TAGTAATTGA AGCTTACTTA CTGATGACCA AGCATGGTGA TCGAGATCCT 
GAGTTAGATG ACCACTGGAG TACATGGGAA GGGACGCCTG TAAAAATAGT GCCTCAGGTG 
GAAACAGTTG ACACACTTAG AAGTATGCAG GTTGAAAATC TACTCTTGGT AGTCATGCAA 
TCTGCTCACC TTGTGATCCA GAGAAAAGCT TTCCAGCAAT CCATTGAAGG GCTTGTAACT 
TTGCACCAAG AACAGACTTC GAGTCAGCCA ATTATTGCCA AAGCTTTGCA GCAGCTAAAG 
GATGATGCAT TGCAGCTGTG CAACAAAATA AGTGATGCCA TTGACCAAGT AGACCACATA 
TTCACATCAG AATTTGATAC TGAAGTTGAT GAGTCAGAGT CTGCCACCTT GCAGCAGTAC 
TATCGTGAAG CCATGATTCA AGGTTACAAT TTTGGTTTTG AGTATCACAA AGAAGTTGTT 
CGTCTGATGT CTGGAACATT TAGACAGAAG ATTGGAGATA AATATATTAG CTTTGCCAGA 
AAGTGGATGA ATTATGTGCT AACTAAATGT GAGAGTGGTC GTGGCACCAG ACCCAGGTGG 
GCAACTCAAG GTTTTGATTT TCTGCAAGCA ATTGAACCAG GCTTTATTTC AGCACTTCCT 
GAAGATGACT TTTTGAGTTT ACAAGCTTTG ATGAATGAAT GCATTGGTCA TGTGATAGGA 
AAACCTCACA GTCCTGTTAC AGGTCTGTAC CTTGCAGCTA TACATCGGAA CAGCCCCCGT 
CCTGTGAAGG TACCCCGATG TCACAGTGAC CCTCCCAACC CACATCTCAT TATCCCAACT 
CCTGATGGAT TCAGCACTCG GAGTGTGCCT TCTGACGTGC GGAACCCATG CAGTGCTGCT 
GCCAGTCGCC CCGCCCCAGC CGGCAGCGAC TCCGCTCCAC TCAAACCTGT CAGCAATACA 
AATGATAACA GGGGATCCAG TGTTCCAGAA AATGATCGTT TGGCCTCGAT AGCTGCTGAA 
TTGCAATTCA GATCATTGAG TCGACATTCA AGCCCCACTG AGGAGCGAGA AGAGCCTTCA 
TACCCAAAAG GAGATTCAAG TGGGCCAACA CGAAGAAGTT GGGAGCTTCG GACTCTGATC 
AGCCAAAATA AAGACACCGC TTCTAAACAA GGACCCATAG AAGCTGTTCA AAAATCAGTT 
CGATTGTTTG AAGAAAAACG TTATCGAGAA ATGAGGAGAA AGAATATCAT TGGTCAAGTT 
TGTGACACCC CCAAATCATA TGATAATGTC ATGCATGTTG GATTACGAAA AGTGACCTTC 
AAATGGCAAA GAGGAAATAA AATTGGAGAA GGCCAGTATG GAAAAGTCTA TACTTGTATC 
AGTGTTGATA CTGGTGAATT GATGGCAATG AAGGAAATTC GATTTCAGCC GAATGACCAC 
AAAACTATCA AAGAGACTGC AGATGAACTG AAAATATTTG AAGGCATTAA GCACCCCAAT 
CTGGTTCGGT ATTTTGGTGT GGAGCTTCAT AGAGAAGAAA TGTATATTTT CATGGAATAT 
TGTGATGAGG GCACCCTGGA AGAAGTGTCA AGGCTGGGCC TTCAAGAACA TGTTATTCGC 
TTGTACTCAA AGCAGATCAC CATTGCTATT AATGTACTGC ATGAGCATGG TATAGTTCAT 
CGTGACATCA AAGGAGCCAA TATCTTCCTT ACATCATCTG GACTAATTAA ATTGGGGGAT 
TTTGGATGCT CAGTCAAGTT AAAAAACAAT GCACAAACCA TGCCTGGAGA AGTGAACAGT 
ACTCTCGGGA CTGCAGCGTA CATGGCACCT GAAGTCATCA CTCGAGCGAA AGGAGAAGGA 
CATGGGCGTG CAGCAGACAT CTGGAGTTTG GGTTGTGTTG TGATAGAAAT GGTTACTGGC 
AAGAGGCCTT GGCATGAGTA TGAGCACAAC TTTCAGATCA TGTATAAGGT TGGAATGGGA 
CATAAGCCAC CCATCCCTGA AAGGATAAGC CCTGAAGGAA AAGATTTCCT TTCCCATTGC 
CTCGAAAGTG ATCCAAAGAT GAGATGGACA GCTAGCCAGC TCCTCGACCA TTCGTTTGTC 
AAGGTTTGCA CAGATGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023359088.1
CDS	1..4764
Translation	MRGAGGRPSAQVLQVRRARRSLLLSPAFLAPPGVQESGPGQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFSTRSVPSDVRNPCSAAASRPAPAGSDSAPLKPVSNTNDNRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERISPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_023503320.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023503320.1

ORF Insert Sequence:

ATGCGGGGAG CTGGGGGTCG CCCCTCCGCT CAGGTGCTGC AGGTCCGCCG GGCTCGGCGC 
AGCCTGCTCC TCAGCCCCGC ATTCTTAGCA CCGCCGGGCG TGCAGGAGTC CGGTCCTGGG 
CAGGAATGCA AGTTGGGAGA TGTTGCTTGC AAAAACTCTG AATCTGATCC AGAGGAGTTC 
TCAGATGAAA CAAATACTGA AAACCTTTAT GGTGCCTCTC CTCCCAGCAC ACCTCGACAG 
ATGAAACGTA TGTCAACAAA ACACCAGAGG AATAATGTAG GGAGACCTTC TGGCCGCTCC 
AGTTTAAAAG AAAAAATGAA TGCACCAAAT CAGCTTCCTC ACAAAGACAT TTGCAAAACA 
GTAGAGAATG TGGAAGAACA CAGTTATAAG CAAGAAAAAA AGATCCGAGC CACTCTTAGA 
ACAACAGAAC GGGATCATAA AAAAAATGTA CAGTGTTCAT TCATGTTAGA CGCAGTGGGT 
GGGTCTTTGC CAAAAAAGCC AATTCCGGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT 
GGCTGTAGCA ATGCTAAGCT TCCGGTTTCT ATGCCTATAC CTATAGCCAG AACTGCACGC 
CAGACTTCCA GGACTGATTG TCCTTTGGAA CGCTTAAAAT TCTTTGAAAC TTTACGACTT 
CTGCTAAAGC TTACCTCAGT CTCAAAGAAA AAAGACAGGG AGCAGAGAGG GCAGGAAAAC 
ACTTCTGCTT TCTGGTTTAA CCGATCCAAT GAACTCATTT GGCTGGAATT ACAAGCCTGG 
CATGCCGGAC GGACCATTAA TGACCAGGAC CTTTTTTTAT ATGCTGCCCG TCAAGCCATC 
CCCGATATTA TCAATGAAAT CCTCACATTC AAAGTAGATT ATGGGAGTTT TGCCTGTGTG 
AGAAATGGAG CTAGTTTTAA TGGTACTTCA GTGGAAGGAC AGAGCAGAGC ACCCCCAGGA 
GCCAATGCTG TGGGTTATTC AAACTATCAC GAACATCTCC AACGCCAGCG GGTCTCCTTT 
GAACAGGTAA AGCGGATAAT GGAGCAGCTT GAATATATAG AAGCACTTTA TCCCTCTTTG 
CAGGCTCTTC AAAAGGACTA TGAAAAATAT GCTGCAAAAG ATTTCCAAGA CAGGGTGCAG 
GCACTCTGTC TGTGGCTAAA CATTACGAAA GACTTAAATC AGAAACTAAG GATTATGGGA 
ACTGTTTTGG GCATCAAAAA TTTATCTGAC ATTGGCTGGC CAGTGTTTGA AATACCGTCT 
CCCCGACCTT CCAAAGGAAA CGAGCCGGAG GAGGAGGGGA GTGACACAGA AGGAGAAGTG 
AAGGAATTGG AAACTACCAC CGATGAAAGT GAAGAAGAAC ATGTTCTAGA TCAGGGTGTC 
AAGGACTTCA GGCGGGCCAT GGAGAGCAGT TTGGACCTCC AGCCCCAGGA CCATTTTTCA 
AGGAAGTTTG ACAGACTGGA GTCTGAAGAT GAATCTATTG GCTGGGGAGC GTCCGAGTAC 
AGTGCTGAAA CAGGCTATAG TAGATACTGT CTGACTTCTA TATATAGACC ATTTGTAGAC 
AAAGCTCTCA AACAGATGGG TTTGAGAAAA TTAATTGTAA GACTTCACAA GCTAATGGAT 
GGTTCTTTAC AAAGGGCCCG TATAGCACTG GTTAAGAGTG ATCATCCATT GGAGTTTTCT 
GAGTTCCCAG ATCCTATGTT GTGTTCAGAT TATATACAGT TGTCAAATAC AACACCGTCA 
TCAGAACAAA AGTGCAGCAC AGTGTCATGG GAAGAGCTGA AATCTATGGA TTTGCCATCA 
TTTGAGCCTG CATTTTTGGT CCTTTGTCGA GTGCTTCTTA ATGTTATCCA TGAGTGTCTC 
AAATTAAGAT TGGAACAGAG ACCTGCTGGG GAACCATCTC TCTTGAGTAT TAAGCAGCTG 
GTAAGAGAGT GTAAAGAGGT TCTGAAGGGT GGCCTGTTAA TGAAGCAATA TTATCAATTT 
ATGCTTCATG AAGTATTAGA TGATTTGGAA AAGATTGACT GCAATATAGA CTCTTTTGAA 
GAGGACCTGC ACAAAATGTT GATGGTGTAT TTTGATTACA TGAGAAGTTG GATCAAAATG 
TTACAGCAGT TACCTCAAGC ATCTCATAGT TTAAAAAATC TATTAGAAGA AGAATGGAAT 
TTCACCAAAG AAATAACTCA TTACATAAGA GGAGGAGAAG CACAGGCTGG AAAATTGTTT 
TGTGACATTG CAGGAATGCT GCTGAAATCT ACTGGAGGAT TTTTAGATTG TGGCCTGCAG 
GAAAGCTGTG CTGAATTCTG GACTAGTGCT GATGATAGCA CTGCTTCAGA TGAAATCAGG 
AGATCTGTTA TAGAGACCAG CAGAGCCCTG AAGGAGCTCT TTCATGAAGC TAGGGAAAGG 
GCTTCCAAAG CACTTGGCTT TGCTAAAATG CTAAGAAAGG ATCTGGAAAT AGCAGCAGAA 
TTCATAGTAT CAGCACCAGT ACAAGACCTT CTGGATATGT TGAAATCAAA ACAATATGTT 
AAGGTGCAAA TTCCTGGATT GGAAAATTTG CAGTTATTTG TTCCACATAC TCTTGCTGGG 
GAGAAATGTG TTATTTTGCA ACTACTGAAT GCAGCTGCAG GAAAGGACTG TTCGAAAGAT 
TCAGATGATG TAGTAATTGA AGCTTACTTA CTGATGACCA AGCATGGTGA TCGAGATCCT 
GAGTTAGATG ACCACTGGAG TACATGGGAA GGGACGCCTG TAAAAATAGT GCCTCAGGTG 
GAAACAGTTG ACACACTTAG AAGTATGCAG GTTGAAAATC TACTCTTGGT AGTCATGCAA 
TCTGCTCACC TTGTGATCCA GAGAAAAGCT TTCCAGCAAT CCATTGAAGG GCTTGTAACT 
TTGCACCAAG AACAGACTTC GAGTCAGCCA ATTATTGCCA AAGCTTTGCA GCAGCTAAAG 
GATGATGCAT TGCAGCTGTG CAACAAAATA AGTGATGCCA TTGACCAAGT AGACCACATA 
TTCACATCAG AATTTGATAC TGAAGTTGAT GAGTCAGAGT CTGCCACCTT GCAGCAGTAC 
TATCGTGAAG CCATGATTCA AGGTTACAAT TTTGGTTTTG AGTATCACAA AGAAGTTGTT 
CGTCTGATGT CTGGAACATT TAGACAGAAG ATTGGAGATA AATATATTAG CTTTGCCAGA 
AAGTGGATGA ATTATGTGCT AACTAAATGT GAGAGTGGTC GTGGCACCAG ACCCAGGTGG 
GCAACTCAAG GTTTTGATTT TCTGCAAGCA ATTGAACCAG GCTTTATTTC AGCACTTCCT 
GAAGATGACT TTTTGAGTTT ACAAGCTTTG ATGAATGAAT GCATTGGTCA TGTGATAGGA 
AAACCTCACA GTCCTGTTAC AGGTCTGTAC CTTGCAGCTA TACATCGGAA CAGCCCCCGT 
CCTGTGAAGG TACCCCGATG TCACAGTGAC CCTCCCAACC CACATCTCAT TATCCCAACT 
CCTGATGGAT TCAGCACTCG GAGTGTGCCT TCTGACGTGC GGAACCCATG CAGTGCTGCT 
GCCAGTCGCC CCGCCCCAGC CGGCAGCGAC TCCGCTCCAC TCAAACCTGT CAGCAATACA 
AATGATAACA GGGGATCCAG TGTTCCAGAA AATGATCGTT TGGCCTCGAT AGCTGCTGAA 
TTGCAATTCA GATCATTGAG TCGACATTCA AGCCCCACTG AGGAGCGAGA AGAGCCTTCA 
TACCCAAAAG GAGATTCAAG TGGGCCAACA CGAAGAAGTT GGGAGCTTCG GACTCTGATC 
AGCCAAAATA AAGACACCGC TTCTAAACAA GGACCCATAG AAGCTGTTCA AAAATCAGTT 
CGATTGTTTG AAGAAAAACG TTATCGAGAA ATGAGGAGAA AGAATATCAT TGGTCAAGTT 
TGTGACACCC CCAAATCATA TGATAATGTC ATGCATGTTG GATTACGAAA AGTGACCTTC 
AAATGGCAAA GAGGAAATAA AATTGGAGAA GGCCAGTATG GAAAAGTCTA TACTTGTATC 
AGTGTTGATA CTGGTGAATT GATGGCAATG AAGGAAATTC GATTTCAGCC GAATGACCAC 
AAAACTATCA AAGAGACTGC AGATGAACTG AAAATATTTG AAGGCATTAA GCACCCCAAT 
CTGGTTCGGT ATTTTGGTGT GGAGCTTCAT AGAGAAGAAA TGTATATTTT CATGGAATAT 
TGTGATGAGG GCACCCTGGA AGAAGTGTCA AGGCTGGGCC TTCAAGAACA TGTTATTCGC 
TTGTACTCAA AGCAGATCAC CATTGCTATT AATGTACTGC ATGAGCATGG TATAGTTCAT 
CGTGACATCA AAGGAGCCAA TATCTTCCTT ACATCATCTG GACTAATTAA ATTGGGGGAT 
TTTGGATGCT CAGTCAAGTT AAAAAACAAT GCACAAACCA TGCCTGGAGA AGTGAACAGT 
ACTCTCGGGA CTGCAGCGTA CATGGCACCT GAAGTCATCA CTCGAGCGAA AGGAGAAGGA 
CATGGGCGTG CAGCAGACAT CTGGAGTTTG GGTTGTGTTG TGATAGAAAT GGTTACTGGC 
AAGAGGCCTT GGCATGAGTA TGAGCACAAC TTTCAGATCA TGTATAAGGT TGGAATGGGA 
CATAAGCCAC CCATCCCTGA AAGGATAAGC CCTGAAGGAA AAGATTTCCT TTCCCATTGC 
CTCGAAAGTG ATCCAAAGAT GAGATGGACA GCTAGCCAGC TCCTCGACCA TTCGTTTGTC 
AAGGTTTGCA CAGATGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_031937521.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031793381.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

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CloneID	OSa236407
Clone ID Related Accession (Same CDS sequence)	XM_031937521.1
Accession Version	XM_031937521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCAGCTATA 
CATCGGAACA GCCCCCGTCC TGTGAAGGTA CCCCGATGTC ACAGTGACCC TCCCAACCCA 
CATCTCATTA TCCCAACTCC TGATGGATTC AGCACTCGGA GTGTGCCTTC TGACGTGCGG 
AACCCATGCA GTGCTGCTGC CAGTCGCCCC GCCCCAGCCG GCAGCGACTC CGCTCCACTC 
AAACCTGTCA GCAATACAAA TGATAACAGG GGATCCAGTG TTCCAGAAAA TGATCGTTTG 
GCCTCGATAG CTGCTGAATT GCAATTCAGA TCATTGAGTC GACATTCAAG CCCCACTGAG 
GAGCGAGAAG AGCCTTCATA CCCAAAAGGA GATTCAAGTG GGCCAACACG AAGAAGTTGG 
GAGCTTCGGA CTCTGATCAG CCAAAATAAA GACACCGCTT CTAAACAAGG ACCCATAGAA 
GCTGTTCAAA AATCAGTTCG ATTGTTTGAA GAAAAACGTT ATCGAGAAAT GAGGAGAAAG 
AATATCATTG GTCAAGTTTG TGACACCCCC AAATCATATG ATAATGTCAT GCATGTTGGA 
TTACGAAAAG TGACCTTCAA ATGGCAAAGA GGAAATAAAA TTGGAGAAGG CCAGTATGGA 
AAAGTCTATA CTTGTATCAG TGTTGATACT GGTGAATTGA TGGCAATGAA GGAAATTCGA 
TTTCAGCCGA ATGACCACAA AACTATCAAA GAGACTGCAG ATGAACTGAA AATATTTGAA 
GGCATTAAGC ACCCCAATCT GGTTCGGTAT TTTGGTGTGG AGCTTCATAG AGAAGAAATG 
TATATTTTCA TGGAATATTG TGATGAGGGC ACCCTGGAAG AAGTGTCAAG GCTGGGCCTT 
CAAGAACATG TTATTCGCTT GTACTCAAAG CAGATCACCA TTGCTATTAA TGTACTGCAT 
GAGCATGGTA TAGTTCATCG TGACATCAAA GGAGCCAATA TCTTCCTTAC ATCATCTGGA 
CTAATTAAAT TGGGGGATTT TGGATGCTCA GTCAAGTTAA AAAACAATGC ACAAACCATG 
CCTGGAGAAG TGAACAGTAC TCTCGGGACT GCAGCGTACA TGGCACCTGA AGTCATCACT 
CGAGCGAAAG GAGAAGGACA TGGGCGTGCA GCAGACATCT GGAGTTTGGG TTGTGTTGTG 
ATAGAAATGG TTACTGGCAA GAGGCCTTGG CATGAGTATG AGCACAACTT TCAGATCATG 
TATAAGGTTG GAATGGGACA TAAGCCACCC ATCCCTGAAA GGATAAGCCC TGAAGGAAAA 
GATTTCCTTT CCCATTGCCT CGAAAGTGAT CCAAAGATGA GATGGACAGC TAGCCAGCTC 
CTCGACCATT CGTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031793381.1
CDS	304..5085
Translation	MREAVAAPELVSPPAGAAAAAANAMEGPPPPPPELPLQLEGCQAARQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFSTRSVPSDVRNPCSAAASRPAPAGSDSAPLKPVSNTNDNRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERISPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_031937521.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031937521.1

ORF Insert Sequence:

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCAGCTATA 
CATCGGAACA GCCCCCGTCC TGTGAAGGTA CCCCGATGTC ACAGTGACCC TCCCAACCCA 
CATCTCATTA TCCCAACTCC TGATGGATTC AGCACTCGGA GTGTGCCTTC TGACGTGCGG 
AACCCATGCA GTGCTGCTGC CAGTCGCCCC GCCCCAGCCG GCAGCGACTC CGCTCCACTC 
AAACCTGTCA GCAATACAAA TGATAACAGG GGATCCAGTG TTCCAGAAAA TGATCGTTTG 
GCCTCGATAG CTGCTGAATT GCAATTCAGA TCATTGAGTC GACATTCAAG CCCCACTGAG 
GAGCGAGAAG AGCCTTCATA CCCAAAAGGA GATTCAAGTG GGCCAACACG AAGAAGTTGG 
GAGCTTCGGA CTCTGATCAG CCAAAATAAA GACACCGCTT CTAAACAAGG ACCCATAGAA 
GCTGTTCAAA AATCAGTTCG ATTGTTTGAA GAAAAACGTT ATCGAGAAAT GAGGAGAAAG 
AATATCATTG GTCAAGTTTG TGACACCCCC AAATCATATG ATAATGTCAT GCATGTTGGA 
TTACGAAAAG TGACCTTCAA ATGGCAAAGA GGAAATAAAA TTGGAGAAGG CCAGTATGGA 
AAAGTCTATA CTTGTATCAG TGTTGATACT GGTGAATTGA TGGCAATGAA GGAAATTCGA 
TTTCAGCCGA ATGACCACAA AACTATCAAA GAGACTGCAG ATGAACTGAA AATATTTGAA 
GGCATTAAGC ACCCCAATCT GGTTCGGTAT TTTGGTGTGG AGCTTCATAG AGAAGAAATG 
TATATTTTCA TGGAATATTG TGATGAGGGC ACCCTGGAAG AAGTGTCAAG GCTGGGCCTT 
CAAGAACATG TTATTCGCTT GTACTCAAAG CAGATCACCA TTGCTATTAA TGTACTGCAT 
GAGCATGGTA TAGTTCATCG TGACATCAAA GGAGCCAATA TCTTCCTTAC ATCATCTGGA 
CTAATTAAAT TGGGGGATTT TGGATGCTCA GTCAAGTTAA AAAACAATGC ACAAACCATG 
CCTGGAGAAG TGAACAGTAC TCTCGGGACT GCAGCGTACA TGGCACCTGA AGTCATCACT 
CGAGCGAAAG GAGAAGGACA TGGGCGTGCA GCAGACATCT GGAGTTTGGG TTGTGTTGTG 
ATAGAAATGG TTACTGGCAA GAGGCCTTGG CATGAGTATG AGCACAACTT TCAGATCATG 
TATAAGGTTG GAATGGGACA TAAGCCACCC ATCCCTGAAA GGATAAGCCC TGAAGGAAAA 
GATTTCCTTT CCCATTGCCT CGAAAGTGAT CCAAAGATGA GATGGACAGC TAGCCAGCTC 
CTCGACCATT CGTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_031937522.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031793382.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

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CloneID	OSa236408
Clone ID Related Accession (Same CDS sequence)	XM_031937522.1
Accession Version	XM_031937522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATACAT 
CGGAACAGCC CCCGTCCTGT GAAGGTACCC CGATGTCACA GTGACCCTCC CAACCCACAT 
CTCATTATCC CAACTCCTGA TGGATTCAGC ACTCGGAGTG TGCCTTCTGA CGTGCGGAAC 
CCATGCAGTG CTGCTGCCAG TCGCCCCGCC CCAGCCGGCA GCGACTCCGC TCCACTCAAA 
CCTGTCAGCA ATACAAATGA TAACAGGGGA TCCAGTGTTC CAGAAAATGA TCGTTTGGCC 
TCGATAGCTG CTGAATTGCA ATTCAGATCA TTGAGTCGAC ATTCAAGCCC CACTGAGGAG 
CGAGAAGAGC CTTCATACCC AAAAGGAGAT TCAAGTGGGC CAACACGAAG AAGTTGGGAG 
CTTCGGACTC TGATCAGCCA AAATAAAGAC ACCGCTTCTA AACAAGGACC CATAGAAGCT 
GTTCAAAAAT CAGTTCGATT GTTTGAAGAA AAACGTTATC GAGAAATGAG GAGAAAGAAT 
ATCATTGGTC AAGTTTGTGA CACCCCCAAA TCATATGATA ATGTCATGCA TGTTGGATTA 
CGAAAAGTGA CCTTCAAATG GCAAAGAGGA AATAAAATTG GAGAAGGCCA GTATGGAAAA 
GTCTATACTT GTATCAGTGT TGATACTGGT GAATTGATGG CAATGAAGGA AATTCGATTT 
CAGCCGAATG ACCACAAAAC TATCAAAGAG ACTGCAGATG AACTGAAAAT ATTTGAAGGC 
ATTAAGCACC CCAATCTGGT TCGGTATTTT GGTGTGGAGC TTCATAGAGA AGAAATGTAT 
ATTTTCATGG AATATTGTGA TGAGGGCACC CTGGAAGAAG TGTCAAGGCT GGGCCTTCAA 
GAACATGTTA TTCGCTTGTA CTCAAAGCAG ATCACCATTG CTATTAATGT ACTGCATGAG 
CATGGTATAG TTCATCGTGA CATCAAAGGA GCCAATATCT TCCTTACATC ATCTGGACTA 
ATTAAATTGG GGGATTTTGG ATGCTCAGTC AAGTTAAAAA ACAATGCACA AACCATGCCT 
GGAGAAGTGA ACAGTACTCT CGGGACTGCA GCGTACATGG CACCTGAAGT CATCACTCGA 
GCGAAAGGAG AAGGACATGG GCGTGCAGCA GACATCTGGA GTTTGGGTTG TGTTGTGATA 
GAAATGGTTA CTGGCAAGAG GCCTTGGCAT GAGTATGAGC ACAACTTTCA GATCATGTAT 
AAGGTTGGAA TGGGACATAA GCCACCCATC CCTGAAAGGA TAAGCCCTGA AGGAAAAGAT 
TTCCTTTCCC ATTGCCTCGA AAGTGATCCA AAGATGAGAT GGACAGCTAG CCAGCTCCTC 
GACCATTCGT TTGTCAAGGT TTGCACAGAT GAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031793382.1
CDS	304..5082
Translation	MREAVAAPELVSPPAGAAAAAANAMEGPPPPPPELPLQLEGCQAARQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFSTRSVPSDVRNPCSAAASRPAPAGSDSAPLKPVSNTNDNRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERISPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_031937522.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031937522.1

ORF Insert Sequence:

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATACAT 
CGGAACAGCC CCCGTCCTGT GAAGGTACCC CGATGTCACA GTGACCCTCC CAACCCACAT 
CTCATTATCC CAACTCCTGA TGGATTCAGC ACTCGGAGTG TGCCTTCTGA CGTGCGGAAC 
CCATGCAGTG CTGCTGCCAG TCGCCCCGCC CCAGCCGGCA GCGACTCCGC TCCACTCAAA 
CCTGTCAGCA ATACAAATGA TAACAGGGGA TCCAGTGTTC CAGAAAATGA TCGTTTGGCC 
TCGATAGCTG CTGAATTGCA ATTCAGATCA TTGAGTCGAC ATTCAAGCCC CACTGAGGAG 
CGAGAAGAGC CTTCATACCC AAAAGGAGAT TCAAGTGGGC CAACACGAAG AAGTTGGGAG 
CTTCGGACTC TGATCAGCCA AAATAAAGAC ACCGCTTCTA AACAAGGACC CATAGAAGCT 
GTTCAAAAAT CAGTTCGATT GTTTGAAGAA AAACGTTATC GAGAAATGAG GAGAAAGAAT 
ATCATTGGTC AAGTTTGTGA CACCCCCAAA TCATATGATA ATGTCATGCA TGTTGGATTA 
CGAAAAGTGA CCTTCAAATG GCAAAGAGGA AATAAAATTG GAGAAGGCCA GTATGGAAAA 
GTCTATACTT GTATCAGTGT TGATACTGGT GAATTGATGG CAATGAAGGA AATTCGATTT 
CAGCCGAATG ACCACAAAAC TATCAAAGAG ACTGCAGATG AACTGAAAAT ATTTGAAGGC 
ATTAAGCACC CCAATCTGGT TCGGTATTTT GGTGTGGAGC TTCATAGAGA AGAAATGTAT 
ATTTTCATGG AATATTGTGA TGAGGGCACC CTGGAAGAAG TGTCAAGGCT GGGCCTTCAA 
GAACATGTTA TTCGCTTGTA CTCAAAGCAG ATCACCATTG CTATTAATGT ACTGCATGAG 
CATGGTATAG TTCATCGTGA CATCAAAGGA GCCAATATCT TCCTTACATC ATCTGGACTA 
ATTAAATTGG GGGATTTTGG ATGCTCAGTC AAGTTAAAAA ACAATGCACA AACCATGCCT 
GGAGAAGTGA ACAGTACTCT CGGGACTGCA GCGTACATGG CACCTGAAGT CATCACTCGA 
GCGAAAGGAG AAGGACATGG GCGTGCAGCA GACATCTGGA GTTTGGGTTG TGTTGTGATA 
GAAATGGTTA CTGGCAAGAG GCCTTGGCAT GAGTATGAGC ACAACTTTCA GATCATGTAT 
AAGGTTGGAA TGGGACATAA GCCACCCATC CCTGAAAGGA TAAGCCCTGA AGGAAAAGAT 
TTCCTTTCCC ATTGCCTCGA AAGTGATCCA AAGATGAGAT GGACAGCTAG CCAGCTCCTC 
GACCATTCGT TTGTCAAGGT TTGCACAGAT GAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_031937523.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031793383.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSa236409
Clone ID Related Accession (Same CDS sequence)	XM_031937523.1
Accession Version	XM_031937523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG CAGCTATACA TCGGAACAGC 
CCCCGTCCTG TGAAGGTACC CCGATGTCAC AGTGACCCTC CCAACCCACA TCTCATTATC 
CCAACTCCTG ATGGATTCAG CACTCGGAGT GTGCCTTCTG ACGTGCGGAA CCCATGCAGT 
GCTGCTGCCA GTCGCCCCGC CCCAGCCGGC AGCGACTCCG CTCCACTCAA ACCTGTCAGC 
AATACAAATG ATAACAGGGG ATCCAGTGTT CCAGAAAATG ATCGTTTGGC CTCGATAGCT 
GCTGAATTGC AATTCAGATC ATTGAGTCGA CATTCAAGCC CCACTGAGGA GCGAGAAGAG 
CCTTCATACC CAAAAGGAGA TTCAAGTGGG CCAACACGAA GAAGTTGGGA GCTTCGGACT 
CTGATCAGCC AAAATAAAGA CACCGCTTCT AAACAAGGAC CCATAGAAGC TGTTCAAAAA 
TCAGTTCGAT TGTTTGAAGA AAAACGTTAT CGAGAAATGA GGAGAAAGAA TATCATTGGT 
CAAGTTTGTG ACACCCCCAA ATCATATGAT AATGTCATGC ATGTTGGATT ACGAAAAGTG 
ACCTTCAAAT GGCAAAGAGG AAATAAAATT GGAGAAGGCC AGTATGGAAA AGTCTATACT 
TGTATCAGTG TTGATACTGG TGAATTGATG GCAATGAAGG AAATTCGATT TCAGCCGAAT 
GACCACAAAA CTATCAAAGA GACTGCAGAT GAACTGAAAA TATTTGAAGG CATTAAGCAC 
CCCAATCTGG TTCGGTATTT TGGTGTGGAG CTTCATAGAG AAGAAATGTA TATTTTCATG 
GAATATTGTG ATGAGGGCAC CCTGGAAGAA GTGTCAAGGC TGGGCCTTCA AGAACATGTT 
ATTCGCTTGT ACTCAAAGCA GATCACCATT GCTATTAATG TACTGCATGA GCATGGTATA 
GTTCATCGTG ACATCAAAGG AGCCAATATC TTCCTTACAT CATCTGGACT AATTAAATTG 
GGGGATTTTG GATGCTCAGT CAAGTTAAAA AACAATGCAC AAACCATGCC TGGAGAAGTG 
AACAGTACTC TCGGGACTGC AGCGTACATG GCACCTGAAG TCATCACTCG AGCGAAAGGA 
GAAGGACATG GGCGTGCAGC AGACATCTGG AGTTTGGGTT GTGTTGTGAT AGAAATGGTT 
ACTGGCAAGA GGCCTTGGCA TGAGTATGAG CACAACTTTC AGATCATGTA TAAGGTTGGA 
ATGGGACATA AGCCACCCAT CCCTGAAAGG ATAAGCCCTG AAGGAAAAGA TTTCCTTTCC 
CATTGCCTCG AAAGTGATCC AAAGATGAGA TGGACAGCTA GCCAGCTCCT CGACCATTCG 
TTTGTCAAGG TTTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031793383.1
CDS	304..5073
Translation	MREAVAAPELVSPPAGAAAAAANAMEGPPPPPPELPLQLEGCQAARQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFSTRSVPSDVRNPCSAAASRPAPAGSDSAPLKPVSNTNDNRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERISPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_031937523.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031937523.1

ORF Insert Sequence:

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG CAGCTATACA TCGGAACAGC 
CCCCGTCCTG TGAAGGTACC CCGATGTCAC AGTGACCCTC CCAACCCACA TCTCATTATC 
CCAACTCCTG ATGGATTCAG CACTCGGAGT GTGCCTTCTG ACGTGCGGAA CCCATGCAGT 
GCTGCTGCCA GTCGCCCCGC CCCAGCCGGC AGCGACTCCG CTCCACTCAA ACCTGTCAGC 
AATACAAATG ATAACAGGGG ATCCAGTGTT CCAGAAAATG ATCGTTTGGC CTCGATAGCT 
GCTGAATTGC AATTCAGATC ATTGAGTCGA CATTCAAGCC CCACTGAGGA GCGAGAAGAG 
CCTTCATACC CAAAAGGAGA TTCAAGTGGG CCAACACGAA GAAGTTGGGA GCTTCGGACT 
CTGATCAGCC AAAATAAAGA CACCGCTTCT AAACAAGGAC CCATAGAAGC TGTTCAAAAA 
TCAGTTCGAT TGTTTGAAGA AAAACGTTAT CGAGAAATGA GGAGAAAGAA TATCATTGGT 
CAAGTTTGTG ACACCCCCAA ATCATATGAT AATGTCATGC ATGTTGGATT ACGAAAAGTG 
ACCTTCAAAT GGCAAAGAGG AAATAAAATT GGAGAAGGCC AGTATGGAAA AGTCTATACT 
TGTATCAGTG TTGATACTGG TGAATTGATG GCAATGAAGG AAATTCGATT TCAGCCGAAT 
GACCACAAAA CTATCAAAGA GACTGCAGAT GAACTGAAAA TATTTGAAGG CATTAAGCAC 
CCCAATCTGG TTCGGTATTT TGGTGTGGAG CTTCATAGAG AAGAAATGTA TATTTTCATG 
GAATATTGTG ATGAGGGCAC CCTGGAAGAA GTGTCAAGGC TGGGCCTTCA AGAACATGTT 
ATTCGCTTGT ACTCAAAGCA GATCACCATT GCTATTAATG TACTGCATGA GCATGGTATA 
GTTCATCGTG ACATCAAAGG AGCCAATATC TTCCTTACAT CATCTGGACT AATTAAATTG 
GGGGATTTTG GATGCTCAGT CAAGTTAAAA AACAATGCAC AAACCATGCC TGGAGAAGTG 
AACAGTACTC TCGGGACTGC AGCGTACATG GCACCTGAAG TCATCACTCG AGCGAAAGGA 
GAAGGACATG GGCGTGCAGC AGACATCTGG AGTTTGGGTT GTGTTGTGAT AGAAATGGTT 
ACTGGCAAGA GGCCTTGGCA TGAGTATGAG CACAACTTTC AGATCATGTA TAAGGTTGGA 
ATGGGACATA AGCCACCCAT CCCTGAAAGG ATAAGCCCTG AAGGAAAAGA TTTCCTTTCC 
CATTGCCTCG AAAGTGATCC AAAGATGAGA TGGACAGCTA GCCAGCTCCT CGACCATTCG 
TTTGTCAAGG TTTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_031937524.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031793384.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSa236410
Clone ID Related Accession (Same CDS sequence)	XM_031937524.1
Accession Version	XM_031937524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG CTATACATCG GAACAGCCCC 
CGTCCTGTGA AGGTACCCCG ATGTCACAGT GACCCTCCCA ACCCACATCT CATTATCCCA 
ACTCCTGATG GATTCAGCAC TCGGAGTGTG CCTTCTGACG TGCGGAACCC ATGCAGTGCT 
GCTGCCAGTC GCCCCGCCCC AGCCGGCAGC GACTCCGCTC CACTCAAACC TGTCAGCAAT 
ACAAATGATA ACAGGGGATC CAGTGTTCCA GAAAATGATC GTTTGGCCTC GATAGCTGCT 
GAATTGCAAT TCAGATCATT GAGTCGACAT TCAAGCCCCA CTGAGGAGCG AGAAGAGCCT 
TCATACCCAA AAGGAGATTC AAGTGGGCCA ACACGAAGAA GTTGGGAGCT TCGGACTCTG 
ATCAGCCAAA ATAAAGACAC CGCTTCTAAA CAAGGACCCA TAGAAGCTGT TCAAAAATCA 
GTTCGATTGT TTGAAGAAAA ACGTTATCGA GAAATGAGGA GAAAGAATAT CATTGGTCAA 
GTTTGTGACA CCCCCAAATC ATATGATAAT GTCATGCATG TTGGATTACG AAAAGTGACC 
TTCAAATGGC AAAGAGGAAA TAAAATTGGA GAAGGCCAGT ATGGAAAAGT CTATACTTGT 
ATCAGTGTTG ATACTGGTGA ATTGATGGCA ATGAAGGAAA TTCGATTTCA GCCGAATGAC 
CACAAAACTA TCAAAGAGAC TGCAGATGAA CTGAAAATAT TTGAAGGCAT TAAGCACCCC 
AATCTGGTTC GGTATTTTGG TGTGGAGCTT CATAGAGAAG AAATGTATAT TTTCATGGAA 
TATTGTGATG AGGGCACCCT GGAAGAAGTG TCAAGGCTGG GCCTTCAAGA ACATGTTATT 
CGCTTGTACT CAAAGCAGAT CACCATTGCT ATTAATGTAC TGCATGAGCA TGGTATAGTT 
CATCGTGACA TCAAAGGAGC CAATATCTTC CTTACATCAT CTGGACTAAT TAAATTGGGG 
GATTTTGGAT GCTCAGTCAA GTTAAAAAAC AATGCACAAA CCATGCCTGG AGAAGTGAAC 
AGTACTCTCG GGACTGCAGC GTACATGGCA CCTGAAGTCA TCACTCGAGC GAAAGGAGAA 
GGACATGGGC GTGCAGCAGA CATCTGGAGT TTGGGTTGTG TTGTGATAGA AATGGTTACT 
GGCAAGAGGC CTTGGCATGA GTATGAGCAC AACTTTCAGA TCATGTATAA GGTTGGAATG 
GGACATAAGC CACCCATCCC TGAAAGGATA AGCCCTGAAG GAAAAGATTT CCTTTCCCAT 
TGCCTCGAAA GTGATCCAAA GATGAGATGG ACAGCTAGCC AGCTCCTCGA CCATTCGTTT 
GTCAAGGTTT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031793384.1
CDS	304..5070
Translation	MREAVAAPELVSPPAGAAAAAANAMEGPPPPPPELPLQLEGCQAARQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFSTRSVPSDVRNPCSAAASRPAPAGSDSAPLKPVSNTNDNRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERISPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_031937524.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031937524.1

ORF Insert Sequence:

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG CTATACATCG GAACAGCCCC 
CGTCCTGTGA AGGTACCCCG ATGTCACAGT GACCCTCCCA ACCCACATCT CATTATCCCA 
ACTCCTGATG GATTCAGCAC TCGGAGTGTG CCTTCTGACG TGCGGAACCC ATGCAGTGCT 
GCTGCCAGTC GCCCCGCCCC AGCCGGCAGC GACTCCGCTC CACTCAAACC TGTCAGCAAT 
ACAAATGATA ACAGGGGATC CAGTGTTCCA GAAAATGATC GTTTGGCCTC GATAGCTGCT 
GAATTGCAAT TCAGATCATT GAGTCGACAT TCAAGCCCCA CTGAGGAGCG AGAAGAGCCT 
TCATACCCAA AAGGAGATTC AAGTGGGCCA ACACGAAGAA GTTGGGAGCT TCGGACTCTG 
ATCAGCCAAA ATAAAGACAC CGCTTCTAAA CAAGGACCCA TAGAAGCTGT TCAAAAATCA 
GTTCGATTGT TTGAAGAAAA ACGTTATCGA GAAATGAGGA GAAAGAATAT CATTGGTCAA 
GTTTGTGACA CCCCCAAATC ATATGATAAT GTCATGCATG TTGGATTACG AAAAGTGACC 
TTCAAATGGC AAAGAGGAAA TAAAATTGGA GAAGGCCAGT ATGGAAAAGT CTATACTTGT 
ATCAGTGTTG ATACTGGTGA ATTGATGGCA ATGAAGGAAA TTCGATTTCA GCCGAATGAC 
CACAAAACTA TCAAAGAGAC TGCAGATGAA CTGAAAATAT TTGAAGGCAT TAAGCACCCC 
AATCTGGTTC GGTATTTTGG TGTGGAGCTT CATAGAGAAG AAATGTATAT TTTCATGGAA 
TATTGTGATG AGGGCACCCT GGAAGAAGTG TCAAGGCTGG GCCTTCAAGA ACATGTTATT 
CGCTTGTACT CAAAGCAGAT CACCATTGCT ATTAATGTAC TGCATGAGCA TGGTATAGTT 
CATCGTGACA TCAAAGGAGC CAATATCTTC CTTACATCAT CTGGACTAAT TAAATTGGGG 
GATTTTGGAT GCTCAGTCAA GTTAAAAAAC AATGCACAAA CCATGCCTGG AGAAGTGAAC 
AGTACTCTCG GGACTGCAGC GTACATGGCA CCTGAAGTCA TCACTCGAGC GAAAGGAGAA 
GGACATGGGC GTGCAGCAGA CATCTGGAGT TTGGGTTGTG TTGTGATAGA AATGGTTACT 
GGCAAGAGGC CTTGGCATGA GTATGAGCAC AACTTTCAGA TCATGTATAA GGTTGGAATG 
GGACATAAGC CACCCATCCC TGAAAGGATA AGCCCTGAAG GAAAAGATTT CCTTTCCCAT 
TGCCTCGAAA GTGATCCAAA GATGAGATGG ACAGCTAGCC AGCTCCTCGA CCATTCGTTT 
GTCAAGGTTT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_031937525.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031793385.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.

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CloneID	OSa236411
Clone ID Related Accession (Same CDS sequence)	XM_031937525.1
Accession Version	XM_031937525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCAGCTATA 
CATCGGAACA GCCCCCGTCC TGTGAAGGTA CCCCGATGTC ACAGTGACCC TCCCAACCCA 
CATCTCATTA TCCCAACTCC TGATGGATTC AGGGGATCCA GTGTTCCAGA AAATGATCGT 
TTGGCCTCGA TAGCTGCTGA ATTGCAATTC AGATCATTGA GTCGACATTC AAGCCCCACT 
GAGGAGCGAG AAGAGCCTTC ATACCCAAAA GGAGATTCAA GTGGGCCAAC ACGAAGAAGT 
TGGGAGCTTC GGACTCTGAT CAGCCAAAAT AAAGACACCG CTTCTAAACA AGGACCCATA 
GAAGCTGTTC AAAAATCAGT TCGATTGTTT GAAGAAAAAC GTTATCGAGA AATGAGGAGA 
AAGAATATCA TTGGTCAAGT TTGTGACACC CCCAAATCAT ATGATAATGT CATGCATGTT 
GGATTACGAA AAGTGACCTT CAAATGGCAA AGAGGAAATA AAATTGGAGA AGGCCAGTAT 
GGAAAAGTCT ATACTTGTAT CAGTGTTGAT ACTGGTGAAT TGATGGCAAT GAAGGAAATT 
CGATTTCAGC CGAATGACCA CAAAACTATC AAAGAGACTG CAGATGAACT GAAAATATTT 
GAAGGCATTA AGCACCCCAA TCTGGTTCGG TATTTTGGTG TGGAGCTTCA TAGAGAAGAA 
ATGTATATTT TCATGGAATA TTGTGATGAG GGCACCCTGG AAGAAGTGTC AAGGCTGGGC 
CTTCAAGAAC ATGTTATTCG CTTGTACTCA AAGCAGATCA CCATTGCTAT TAATGTACTG 
CATGAGCATG GTATAGTTCA TCGTGACATC AAAGGAGCCA ATATCTTCCT TACATCATCT 
GGACTAATTA AATTGGGGGA TTTTGGATGC TCAGTCAAGT TAAAAAACAA TGCACAAACC 
ATGCCTGGAG AAGTGAACAG TACTCTCGGG ACTGCAGCGT ACATGGCACC TGAAGTCATC 
ACTCGAGCGA AAGGAGAAGG ACATGGGCGT GCAGCAGACA TCTGGAGTTT GGGTTGTGTT 
GTGATAGAAA TGGTTACTGG CAAGAGGCCT TGGCATGAGT ATGAGCACAA CTTTCAGATC 
ATGTATAAGG TTGGAATGGG ACATAAGCCA CCCATCCCTG AAAGGATAAG CCCTGAAGGA 
AAAGATTTCC TTTCCCATTG CCTCGAAAGT GATCCAAAGA TGAGATGGAC AGCTAGCCAG 
CTCCTCGACC ATTCGTTTGT CAAGGTTTGC ACAGATGAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031793385.1
CDS	304..4968
Translation	MREAVAAPELVSPPAGAAAAAANAMEGPPPPPPELPLQLEGCQAARQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERISPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_031937525.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031937525.1

ORF Insert Sequence:

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCAGCTATA 
CATCGGAACA GCCCCCGTCC TGTGAAGGTA CCCCGATGTC ACAGTGACCC TCCCAACCCA 
CATCTCATTA TCCCAACTCC TGATGGATTC AGGGGATCCA GTGTTCCAGA AAATGATCGT 
TTGGCCTCGA TAGCTGCTGA ATTGCAATTC AGATCATTGA GTCGACATTC AAGCCCCACT 
GAGGAGCGAG AAGAGCCTTC ATACCCAAAA GGAGATTCAA GTGGGCCAAC ACGAAGAAGT 
TGGGAGCTTC GGACTCTGAT CAGCCAAAAT AAAGACACCG CTTCTAAACA AGGACCCATA 
GAAGCTGTTC AAAAATCAGT TCGATTGTTT GAAGAAAAAC GTTATCGAGA AATGAGGAGA 
AAGAATATCA TTGGTCAAGT TTGTGACACC CCCAAATCAT ATGATAATGT CATGCATGTT 
GGATTACGAA AAGTGACCTT CAAATGGCAA AGAGGAAATA AAATTGGAGA AGGCCAGTAT 
GGAAAAGTCT ATACTTGTAT CAGTGTTGAT ACTGGTGAAT TGATGGCAAT GAAGGAAATT 
CGATTTCAGC CGAATGACCA CAAAACTATC AAAGAGACTG CAGATGAACT GAAAATATTT 
GAAGGCATTA AGCACCCCAA TCTGGTTCGG TATTTTGGTG TGGAGCTTCA TAGAGAAGAA 
ATGTATATTT TCATGGAATA TTGTGATGAG GGCACCCTGG AAGAAGTGTC AAGGCTGGGC 
CTTCAAGAAC ATGTTATTCG CTTGTACTCA AAGCAGATCA CCATTGCTAT TAATGTACTG 
CATGAGCATG GTATAGTTCA TCGTGACATC AAAGGAGCCA ATATCTTCCT TACATCATCT 
GGACTAATTA AATTGGGGGA TTTTGGATGC TCAGTCAAGT TAAAAAACAA TGCACAAACC 
ATGCCTGGAG AAGTGAACAG TACTCTCGGG ACTGCAGCGT ACATGGCACC TGAAGTCATC 
ACTCGAGCGA AAGGAGAAGG ACATGGGCGT GCAGCAGACA TCTGGAGTTT GGGTTGTGTT 
GTGATAGAAA TGGTTACTGG CAAGAGGCCT TGGCATGAGT ATGAGCACAA CTTTCAGATC 
ATGTATAAGG TTGGAATGGG ACATAAGCCA CCCATCCCTG AAAGGATAAG CCCTGAAGGA 
AAAGATTTCC TTTCCCATTG CCTCGAAAGT GATCCAAAGA TGAGATGGAC AGCTAGCCAG 
CTCCTCGACC ATTCGTTTGT CAAGGTTTGC ACAGATGAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_031937526.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031793386.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

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CloneID	OSa236412
Clone ID Related Accession (Same CDS sequence)	XM_031937526.1
Accession Version	XM_031937526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATACAT 
CGGAACAGCC CCCGTCCTGT GAAGGTACCC CGATGTCACA GTGACCCTCC CAACCCACAT 
CTCATTATCC CAACTCCTGA TGGATTCAGG GGATCCAGTG TTCCAGAAAA TGATCGTTTG 
GCCTCGATAG CTGCTGAATT GCAATTCAGA TCATTGAGTC GACATTCAAG CCCCACTGAG 
GAGCGAGAAG AGCCTTCATA CCCAAAAGGA GATTCAAGTG GGCCAACACG AAGAAGTTGG 
GAGCTTCGGA CTCTGATCAG CCAAAATAAA GACACCGCTT CTAAACAAGG ACCCATAGAA 
GCTGTTCAAA AATCAGTTCG ATTGTTTGAA GAAAAACGTT ATCGAGAAAT GAGGAGAAAG 
AATATCATTG GTCAAGTTTG TGACACCCCC AAATCATATG ATAATGTCAT GCATGTTGGA 
TTACGAAAAG TGACCTTCAA ATGGCAAAGA GGAAATAAAA TTGGAGAAGG CCAGTATGGA 
AAAGTCTATA CTTGTATCAG TGTTGATACT GGTGAATTGA TGGCAATGAA GGAAATTCGA 
TTTCAGCCGA ATGACCACAA AACTATCAAA GAGACTGCAG ATGAACTGAA AATATTTGAA 
GGCATTAAGC ACCCCAATCT GGTTCGGTAT TTTGGTGTGG AGCTTCATAG AGAAGAAATG 
TATATTTTCA TGGAATATTG TGATGAGGGC ACCCTGGAAG AAGTGTCAAG GCTGGGCCTT 
CAAGAACATG TTATTCGCTT GTACTCAAAG CAGATCACCA TTGCTATTAA TGTACTGCAT 
GAGCATGGTA TAGTTCATCG TGACATCAAA GGAGCCAATA TCTTCCTTAC ATCATCTGGA 
CTAATTAAAT TGGGGGATTT TGGATGCTCA GTCAAGTTAA AAAACAATGC ACAAACCATG 
CCTGGAGAAG TGAACAGTAC TCTCGGGACT GCAGCGTACA TGGCACCTGA AGTCATCACT 
CGAGCGAAAG GAGAAGGACA TGGGCGTGCA GCAGACATCT GGAGTTTGGG TTGTGTTGTG 
ATAGAAATGG TTACTGGCAA GAGGCCTTGG CATGAGTATG AGCACAACTT TCAGATCATG 
TATAAGGTTG GAATGGGACA TAAGCCACCC ATCCCTGAAA GGATAAGCCC TGAAGGAAAA 
GATTTCCTTT CCCATTGCCT CGAAAGTGAT CCAAAGATGA GATGGACAGC TAGCCAGCTC 
CTCGACCATT CGTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031793386.1
CDS	304..4965
Translation	MREAVAAPELVSPPAGAAAAAANAMEGPPPPPPELPLQLEGCQAARQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERISPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_031937526.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031937526.1

ORF Insert Sequence:

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATACAT 
CGGAACAGCC CCCGTCCTGT GAAGGTACCC CGATGTCACA GTGACCCTCC CAACCCACAT 
CTCATTATCC CAACTCCTGA TGGATTCAGG GGATCCAGTG TTCCAGAAAA TGATCGTTTG 
GCCTCGATAG CTGCTGAATT GCAATTCAGA TCATTGAGTC GACATTCAAG CCCCACTGAG 
GAGCGAGAAG AGCCTTCATA CCCAAAAGGA GATTCAAGTG GGCCAACACG AAGAAGTTGG 
GAGCTTCGGA CTCTGATCAG CCAAAATAAA GACACCGCTT CTAAACAAGG ACCCATAGAA 
GCTGTTCAAA AATCAGTTCG ATTGTTTGAA GAAAAACGTT ATCGAGAAAT GAGGAGAAAG 
AATATCATTG GTCAAGTTTG TGACACCCCC AAATCATATG ATAATGTCAT GCATGTTGGA 
TTACGAAAAG TGACCTTCAA ATGGCAAAGA GGAAATAAAA TTGGAGAAGG CCAGTATGGA 
AAAGTCTATA CTTGTATCAG TGTTGATACT GGTGAATTGA TGGCAATGAA GGAAATTCGA 
TTTCAGCCGA ATGACCACAA AACTATCAAA GAGACTGCAG ATGAACTGAA AATATTTGAA 
GGCATTAAGC ACCCCAATCT GGTTCGGTAT TTTGGTGTGG AGCTTCATAG AGAAGAAATG 
TATATTTTCA TGGAATATTG TGATGAGGGC ACCCTGGAAG AAGTGTCAAG GCTGGGCCTT 
CAAGAACATG TTATTCGCTT GTACTCAAAG CAGATCACCA TTGCTATTAA TGTACTGCAT 
GAGCATGGTA TAGTTCATCG TGACATCAAA GGAGCCAATA TCTTCCTTAC ATCATCTGGA 
CTAATTAAAT TGGGGGATTT TGGATGCTCA GTCAAGTTAA AAAACAATGC ACAAACCATG 
CCTGGAGAAG TGAACAGTAC TCTCGGGACT GCAGCGTACA TGGCACCTGA AGTCATCACT 
CGAGCGAAAG GAGAAGGACA TGGGCGTGCA GCAGACATCT GGAGTTTGGG TTGTGTTGTG 
ATAGAAATGG TTACTGGCAA GAGGCCTTGG CATGAGTATG AGCACAACTT TCAGATCATG 
TATAAGGTTG GAATGGGACA TAAGCCACCC ATCCCTGAAA GGATAAGCCC TGAAGGAAAA 
GATTTCCTTT CCCATTGCCT CGAAAGTGAT CCAAAGATGA GATGGACAGC TAGCCAGCTC 
CTCGACCATT CGTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_031937527.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031793387.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.

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CloneID	OSa236413
Clone ID Related Accession (Same CDS sequence)	XM_031937527.1
Accession Version	XM_031937527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG CTATACATCG GAACAGCCCC 
CGTCCTGTGA AGGTACCCCG ATGTCACAGT GACCCTCCCA ACCCACATCT CATTATCCCA 
ACTCCTGATG GATTCAGGGG ATCCAGTGTT CCAGAAAATG ATCGTTTGGC CTCGATAGCT 
GCTGAATTGC AATTCAGATC ATTGAGTCGA CATTCAAGCC CCACTGAGGA GCGAGAAGAG 
CCTTCATACC CAAAAGGAGA TTCAAGTGGG CCAACACGAA GAAGTTGGGA GCTTCGGACT 
CTGATCAGCC AAAATAAAGA CACCGCTTCT AAACAAGGAC CCATAGAAGC TGTTCAAAAA 
TCAGTTCGAT TGTTTGAAGA AAAACGTTAT CGAGAAATGA GGAGAAAGAA TATCATTGGT 
CAAGTTTGTG ACACCCCCAA ATCATATGAT AATGTCATGC ATGTTGGATT ACGAAAAGTG 
ACCTTCAAAT GGCAAAGAGG AAATAAAATT GGAGAAGGCC AGTATGGAAA AGTCTATACT 
TGTATCAGTG TTGATACTGG TGAATTGATG GCAATGAAGG AAATTCGATT TCAGCCGAAT 
GACCACAAAA CTATCAAAGA GACTGCAGAT GAACTGAAAA TATTTGAAGG CATTAAGCAC 
CCCAATCTGG TTCGGTATTT TGGTGTGGAG CTTCATAGAG AAGAAATGTA TATTTTCATG 
GAATATTGTG ATGAGGGCAC CCTGGAAGAA GTGTCAAGGC TGGGCCTTCA AGAACATGTT 
ATTCGCTTGT ACTCAAAGCA GATCACCATT GCTATTAATG TACTGCATGA GCATGGTATA 
GTTCATCGTG ACATCAAAGG AGCCAATATC TTCCTTACAT CATCTGGACT AATTAAATTG 
GGGGATTTTG GATGCTCAGT CAAGTTAAAA AACAATGCAC AAACCATGCC TGGAGAAGTG 
AACAGTACTC TCGGGACTGC AGCGTACATG GCACCTGAAG TCATCACTCG AGCGAAAGGA 
GAAGGACATG GGCGTGCAGC AGACATCTGG AGTTTGGGTT GTGTTGTGAT AGAAATGGTT 
ACTGGCAAGA GGCCTTGGCA TGAGTATGAG CACAACTTTC AGATCATGTA TAAGGTTGGA 
ATGGGACATA AGCCACCCAT CCCTGAAAGG ATAAGCCCTG AAGGAAAAGA TTTCCTTTCC 
CATTGCCTCG AAAGTGATCC AAAGATGAGA TGGACAGCTA GCCAGCTCCT CGACCATTCG 
TTTGTCAAGG TTTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031793387.1
CDS	304..4953
Translation	MREAVAAPELVSPPAGAAAAAANAMEGPPPPPPELPLQLEGCQAARQECKLGDVACKNSESDPEEFSDETNTENLYGASPPSTPRQMKRMSTKHQRNNVGRPSGRSSLKEKMNAPNQLPHKDICKTVENVEEHSYKQEKKIRATLRTTERDHKKNVQCSFMLDAVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSMPIPIARTARQTSRTDCPLERLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYAARQAIPDIINEILTFKVDYGSFACVRNGASFNGTSVEGQSRAPPGANAVGYSNYHEHLQRQRVSFEQVKRIMEQLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEEEGSDTEGEVKELETTTDESEEEHVLDQGVKDFRRAMESSLDLQPQDHFSRKFDRLESEDESIGWGASEYSAETGYSRYCLTSIYRPFVDKALKQMGLRKLIVRLHKLMDGSLQRARIALVKSDHPLEFSEFPDPMLCSDYIQLSNTTPSSEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLDDLEKIDCNIDSFEEDLHKMLMVYFDYMRSWIKMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGGFLDCGLQESCAEFWTSADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFIVSAPVQDLLDMLKSKQYVKVQIPGLENLQLFVPHTLAGEKCVILQLLNAAAGKDCSKDSDDVVIEAYLLMTKHGDRDPELDDHWSTWEGTPVKIVPQVETVDTLRSMQVENLLLVVMQSAHLVIQRKAFQQSIEGLVTLHQEQTSSQPIIAKALQQLKDDALQLCNKISDAIDQVDHIFTSEFDTEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGTFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPGFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPDGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPSYPKGDSSGPTRRSWELRTLISQNKDTASKQGPIEAVQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERISPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_031937527.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031937527.1

ORF Insert Sequence:

ATGAGAGAGG CAGTCGCAGC TCCCGAGCTG GTCTCCCCTC CCGCAGGAGC CGCCGCCGCC 
GCCGCCAACG CCATGGAGGG GCCGCCGCCG CCGCCGCCGG AGCTGCCGCT GCAGCTGGAG 
GGTTGCCAGG CGGCGAGGCA GGAATGCAAG TTGGGAGATG TTGCTTGCAA AAACTCTGAA 
TCTGATCCAG AGGAGTTCTC AGATGAAACA AATACTGAAA ACCTTTATGG TGCCTCTCCT 
CCCAGCACAC CTCGACAGAT GAAACGTATG TCAACAAAAC ACCAGAGGAA TAATGTAGGG 
AGACCTTCTG GCCGCTCCAG TTTAAAAGAA AAAATGAATG CACCAAATCA GCTTCCTCAC 
AAAGACATTT GCAAAACAGT AGAGAATGTG GAAGAACACA GTTATAAGCA AGAAAAAAAG 
ATCCGAGCCA CTCTTAGAAC AACAGAACGG GATCATAAAA AAAATGTACA GTGTTCATTC 
ATGTTAGACG CAGTGGGTGG GTCTTTGCCA AAAAAGCCAA TTCCGGATGT TGATCTCAAT 
AAGCCTTACC TCAGCCTTGG CTGTAGCAAT GCTAAGCTTC CGGTTTCTAT GCCTATACCT 
ATAGCCAGAA CTGCACGCCA GACTTCCAGG ACTGATTGTC CTTTGGAACG CTTAAAATTC 
TTTGAAACTT TACGACTTCT GCTAAAGCTT ACCTCAGTCT CAAAGAAAAA AGACAGGGAG 
CAGAGAGGGC AGGAAAACAC TTCTGCTTTC TGGTTTAACC GATCCAATGA ACTCATTTGG 
CTGGAATTAC AAGCCTGGCA TGCCGGACGG ACCATTAATG ACCAGGACCT TTTTTTATAT 
GCTGCCCGTC AAGCCATCCC CGATATTATC AATGAAATCC TCACATTCAA AGTAGATTAT 
GGGAGTTTTG CCTGTGTGAG AAATGGAGCT AGTTTTAATG GTACTTCAGT GGAAGGACAG 
AGCAGAGCAC CCCCAGGAGC CAATGCTGTG GGTTATTCAA ACTATCACGA ACATCTCCAA 
CGCCAGCGGG TCTCCTTTGA ACAGGTAAAG CGGATAATGG AGCAGCTTGA ATATATAGAA 
GCACTTTATC CCTCTTTGCA GGCTCTTCAA AAGGACTATG AAAAATATGC TGCAAAAGAT 
TTCCAAGACA GGGTGCAGGC ACTCTGTCTG TGGCTAAACA TTACGAAAGA CTTAAATCAG 
AAACTAAGGA TTATGGGAAC TGTTTTGGGC ATCAAAAATT TATCTGACAT TGGCTGGCCA 
GTGTTTGAAA TACCGTCTCC CCGACCTTCC AAAGGAAACG AGCCGGAGGA GGAGGGGAGT 
GACACAGAAG GAGAAGTGAA GGAATTGGAA ACTACCACCG ATGAAAGTGA AGAAGAACAT 
GTTCTAGATC AGGGTGTCAA GGACTTCAGG CGGGCCATGG AGAGCAGTTT GGACCTCCAG 
CCCCAGGACC ATTTTTCAAG GAAGTTTGAC AGACTGGAGT CTGAAGATGA ATCTATTGGC 
TGGGGAGCGT CCGAGTACAG TGCTGAAACA GGCTATAGTA GATACTGTCT GACTTCTATA 
TATAGACCAT TTGTAGACAA AGCTCTCAAA CAGATGGGTT TGAGAAAATT AATTGTAAGA 
CTTCACAAGC TAATGGATGG TTCTTTACAA AGGGCCCGTA TAGCACTGGT TAAGAGTGAT 
CATCCATTGG AGTTTTCTGA GTTCCCAGAT CCTATGTTGT GTTCAGATTA TATACAGTTG 
TCAAATACAA CACCGTCATC AGAACAAAAG TGCAGCACAG TGTCATGGGA AGAGCTGAAA 
TCTATGGATT TGCCATCATT TGAGCCTGCA TTTTTGGTCC TTTGTCGAGT GCTTCTTAAT 
GTTATCCATG AGTGTCTCAA ATTAAGATTG GAACAGAGAC CTGCTGGGGA ACCATCTCTC 
TTGAGTATTA AGCAGCTGGT AAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ATCAATTTAT GCTTCATGAA GTATTAGATG ATTTGGAAAA GATTGACTGC 
AATATAGACT CTTTTGAAGA GGACCTGCAC AAAATGTTGA TGGTGTATTT TGATTACATG 
AGAAGTTGGA TCAAAATGTT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTA 
TTAGAAGAAG AATGGAATTT CACCAAAGAA ATAACTCATT ACATAAGAGG AGGAGAAGCA 
CAGGCTGGAA AATTGTTTTG TGACATTGCA GGAATGCTGC TGAAATCTAC TGGAGGATTT 
TTAGATTGTG GCCTGCAGGA AAGCTGTGCT GAATTCTGGA CTAGTGCTGA TGATAGCACT 
GCTTCAGATG AAATCAGGAG ATCTGTTATA GAGACCAGCA GAGCCCTGAA GGAGCTCTTT 
CATGAAGCTA GGGAAAGGGC TTCCAAAGCA CTTGGCTTTG CTAAAATGCT AAGAAAGGAT 
CTGGAAATAG CAGCAGAATT CATAGTATCA GCACCAGTAC AAGACCTTCT GGATATGTTG 
AAATCAAAAC AATATGTTAA GGTGCAAATT CCTGGATTGG AAAATTTGCA GTTATTTGTT 
CCACATACTC TTGCTGGGGA GAAATGTGTT ATTTTGCAAC TACTGAATGC AGCTGCAGGA 
AAGGACTGTT CGAAAGATTC AGATGATGTA GTAATTGAAG CTTACTTACT GATGACCAAG 
CATGGTGATC GAGATCCTGA GTTAGATGAC CACTGGAGTA CATGGGAAGG GACGCCTGTA 
AAAATAGTGC CTCAGGTGGA AACAGTTGAC ACACTTAGAA GTATGCAGGT TGAAAATCTA 
CTCTTGGTAG TCATGCAATC TGCTCACCTT GTGATCCAGA GAAAAGCTTT CCAGCAATCC 
ATTGAAGGGC TTGTAACTTT GCACCAAGAA CAGACTTCGA GTCAGCCAAT TATTGCCAAA 
GCTTTGCAGC AGCTAAAGGA TGATGCATTG CAGCTGTGCA ACAAAATAAG TGATGCCATT 
GACCAAGTAG ACCACATATT CACATCAGAA TTTGATACTG AAGTTGATGA GTCAGAGTCT 
GCCACCTTGC AGCAGTACTA TCGTGAAGCC ATGATTCAAG GTTACAATTT TGGTTTTGAG 
TATCACAAAG AAGTTGTTCG TCTGATGTCT GGAACATTTA GACAGAAGAT TGGAGATAAA 
TATATTAGCT TTGCCAGAAA GTGGATGAAT TATGTGCTAA CTAAATGTGA GAGTGGTCGT 
GGCACCAGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TGCAAGCAAT TGAACCAGGC 
TTTATTTCAG CACTTCCTGA AGATGACTTT TTGAGTTTAC AAGCTTTGAT GAATGAATGC 
ATTGGTCATG TGATAGGAAA ACCTCACAGT CCTGTTACAG CTATACATCG GAACAGCCCC 
CGTCCTGTGA AGGTACCCCG ATGTCACAGT GACCCTCCCA ACCCACATCT CATTATCCCA 
ACTCCTGATG GATTCAGGGG ATCCAGTGTT CCAGAAAATG ATCGTTTGGC CTCGATAGCT 
GCTGAATTGC AATTCAGATC ATTGAGTCGA CATTCAAGCC CCACTGAGGA GCGAGAAGAG 
CCTTCATACC CAAAAGGAGA TTCAAGTGGG CCAACACGAA GAAGTTGGGA GCTTCGGACT 
CTGATCAGCC AAAATAAAGA CACCGCTTCT AAACAAGGAC CCATAGAAGC TGTTCAAAAA 
TCAGTTCGAT TGTTTGAAGA AAAACGTTAT CGAGAAATGA GGAGAAAGAA TATCATTGGT 
CAAGTTTGTG ACACCCCCAA ATCATATGAT AATGTCATGC ATGTTGGATT ACGAAAAGTG 
ACCTTCAAAT GGCAAAGAGG AAATAAAATT GGAGAAGGCC AGTATGGAAA AGTCTATACT 
TGTATCAGTG TTGATACTGG TGAATTGATG GCAATGAAGG AAATTCGATT TCAGCCGAAT 
GACCACAAAA CTATCAAAGA GACTGCAGAT GAACTGAAAA TATTTGAAGG CATTAAGCAC 
CCCAATCTGG TTCGGTATTT TGGTGTGGAG CTTCATAGAG AAGAAATGTA TATTTTCATG 
GAATATTGTG ATGAGGGCAC CCTGGAAGAA GTGTCAAGGC TGGGCCTTCA AGAACATGTT 
ATTCGCTTGT ACTCAAAGCA GATCACCATT GCTATTAATG TACTGCATGA GCATGGTATA 
GTTCATCGTG ACATCAAAGG AGCCAATATC TTCCTTACAT CATCTGGACT AATTAAATTG 
GGGGATTTTG GATGCTCAGT CAAGTTAAAA AACAATGCAC AAACCATGCC TGGAGAAGTG 
AACAGTACTC TCGGGACTGC AGCGTACATG GCACCTGAAG TCATCACTCG AGCGAAAGGA 
GAAGGACATG GGCGTGCAGC AGACATCTGG AGTTTGGGTT GTGTTGTGAT AGAAATGGTT 
ACTGGCAAGA GGCCTTGGCA TGAGTATGAG CACAACTTTC AGATCATGTA TAAGGTTGGA 
ATGGGACATA AGCCACCCAT CCCTGAAAGG ATAAGCCCTG AAGGAAAAGA TTTCCTTTCC 
CATTGCCTCG AAAGTGATCC AAAGATGAGA TGGACAGCTA GCCAGCTCCT CGACCATTCG 
TTTGTCAAGG TTTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MAP3K4
Entrez Gene ID	100928208
Full Name	mitogen-activated protein kinase kinase kinase 4
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MAP3K4 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

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