CACNA1I cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1I
Entrez Gene ID	100916832
Full Name	calcium voltage-gated channel subunit alpha1 I
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003770996.1	XP_003771044.1	voltage-dependent T-type calcium channel subunit alpha-1I
XM_031940123.1	XP_031795983.1	voltage-dependent T-type calcium channel subunit alpha-1I
XM_023504351.1	XP_023360119.1	voltage-dependent T-type calcium channel subunit alpha-1I

Pathways from BioSystems
Gene Ontology

The following CACNA1I gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1I cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa133176	XM_003770996.1 Latest version!	Sarcophilus harrisii calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa155935	XM_023504351.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa238048	XM_031940123.1 Latest version!	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1I ( XM_003770996.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003771044.1 Sarcophilus harrisii calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), mRNA.

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CloneID	OSa133176
Clone ID Related Accession (Same CDS sequence)	XM_003770996.1
Accession Version	XM_003770996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843601.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 17% of CDS bases ##RefSeq-Attributes-END##

ATGGCCGAGG GTGTTCTGCC CGCTGCTGCC TGGAGCCCCA GCCCCCTGCC CCCCGGGCGC 
TCCGTGCCTG ATCTTGCCTC AGAGCCTGGA GTCACGGAGC AGCAGCCCCA GCCTCGGACC 
CCACCTTCCT CTCCGGCCCC GGAGCCCGGC CCCGGCACTG ACCCTGACGT CCCACACCCC 
GATCTGGCCC CCGTCGTCTT TTTTTGTCTC CGACAAACCA CCAGCCCTCG AAACTGGTGC 
ATCAAGATGG TGTGTAATCC TTGGTTCGAG TGTGTCAGCA TGTTGGTGAT TCTCCTCAAC 
TGTGTCACCC TGGGCATGTA CCAGCCATGT GAGGACATGG ATTGTCTGTC TGACCGCTGC 
AAGATCCTGC AGGTTTTTGA TGACTTCATC TTCATCTTCT TTGCCATGGA GATGGTGCTG 
AAGATGGTGG CCCTGGGGAT TTTCGGCAGG AAGTGTTACC TGGGGGACAC CTGGAACCGA 
CTGGATTTCT TCATTGTGAT GGCCGGGATG GTGGAATATT CTCTGGACCT TCAGAACATC 
AACCTGTCAG CCATCCGCAC AGTCCGAGTC CTGAGGCCCC TCAAAGCCAT CAATCGCGTG 
CCCAGCCTGC CCCCTCCGGA CCCTTCCTGC TGGGACCCCC GGGTGAGCCG GCCCCACCTG 
GAATATGACG ATCCAGGCGT AGCCGATGTT GTCGAAGTTG ATGGCGCCCT TGTGGGGGTT 
GACGCTGCCC GTGCGGCACA CGTTGTAATA GCGGTTCCAG TTGACACACA TGCCGCTGAT 
GTTGAAGTCC TGCTGGGCCG CGCTGTAGAA GGCGAAGTCG TCCTTGCTCA GGCAGCACTC 
CCGGCCCTGC TCCTTGAGCG GGGGGATTTC GTGGCAGCCC ATGATGCCGT TGTCCCCCGA 
GAGCGAGCAG ATGAACGGCA TCTCGTCATC CTCCTCCGGC TGAAAGCAGC GGTTCCGAAG 
CAGGCCGGCC CACAGCTGCA CACCGATTAT CCCAAAGATG AAGAAGACAA AGAAACACAA 
CAGCAGTACG TTGCCCAACA TGGGCAGGGT GTCCAGCAGC AGGTTCACGA GGATGCGCAT 
ACCTGTGGAG GAGAGGGTGA AGGGAAGGCT ATGGCCTCCA CAAACCACCT CACAATTGCC 
TGCTTACATG TCTCCTCGCA GAGTGTGGGC TCCTTCTTCA TGATCAACCT GTGCCTCGTT 
GTCATAGCGA CCCAGTTCTC CGAGACCAAG CAGAGAGAGC ACCAGCTGAT GCAGGAGCAG 
CGCAGGCGGT ACCTGTCCTC CAGCACCGTG GCCAGCTACG CAGAGCCCGG AGATTGCTAT 
GAGGAGATAT TCCAGTATGT CTGCCACATT CTGCGAAAAG CCAAGCGCCG AAGCCTGGGC 
CTCTACCATG CCCTCCGCAG CCGCCACCAG GGCCAGGTGG AGCCTGGTGT GGGTGCCAAG 
CCCGAGGCAG GGAGTGCCAA GGAGCCGAGG AGGGACAGGC TATGTCAGAA ACACATTCCC 
TTGGATTACC CATCCCATAC TCTCGTGCAA CCCATCTCTG TGACACAGAC CTCCGACCCT 
GGCAACTGCC CCCATTGCCA GCAGGAGAGA GATCAGCGTC TATCCCTGGC AGAGAGTGAT 
GATGACTTTG GGCGGGAGGA AGGTTCTGCC AATGATGATG AAGGCGAAGG TGGAGGAAGC 
AGTGAAGGAA CTCTGGACCT AGAGAAGGAG GAAGAGGAGG GAGAAAAAGG CAGTGGCGCA 
GCCAGCCTGT GTGGGGATAT ATGGCGGGAG GTGAGGGCCA AGCTGAGGGG CATCGTGGAC 
AGCAAATATT TTAACCGAGG CATCATGATT GCCATCTTGG TGAACACTGT CAGCATGGGC 
ATTGAACACC ACGAACAGCC CGAGGAGCTG ACCAACATCC TGGAGATCAG TAACGTGGTG 
TTCACCAGCA TGTTTGCCCT GGAGATGATC CTGAAGCTGG CGGCCTTCGG CCTCTTCGAC 
TACCTACGGA ACCCTTACAA CATCTTTGAC AGTATCATCG TCATCATCAG TATCTGGGAG 
ATCATCGGCC AGTCGGACGG GGGCCTCTCG GTACTGCGGA CGTTCCGCCT GCTTCGAGTC 
CTGAAGCTGG TCCGCTTCAT GCCAGCCCTG CGCCGCCAGC TGGTGGTTTT GATGAAGACC 
ATGGACAACG TGGCCACCTT CTGCATGCTG CTCATGCTCT TCATCTTCAT CTTCAGCATC 
CTAGGGATGC ACATTTTTGG CTGCAAATTC AGCCTCAAGA CTGACACGGG AGACACTGTA 
CCCGACCGGA AGAACTTTGA TTCGCTTCTT TGGGCCATTG TCACAGTGTT CCAGATCCTG 
ACCCAAGAGG ACTGGAATAT GGTGCTCTAC AATGGCATGG CCTCCACCTC TCCTTGGGCC 
TCCCTCTACT TTGTGGCTCT CATGACCTTC GGCAACTACG TGCTCTTCAA CCTGCTGGTG 
GCCATCCTGG TGGAGGGCTT CCAGGCAGAG GGGGATGCCA ACCGCTCCTA CTCAGATGAG 
GACCAGAGCT CGTCCAACAT GGAGGAGTTT GACAAGTTCC GAGAGGGGCA TGAAGGCCAT 
TTTGATATCA AGCTCTGCCC CATCCCCATG ACTCCCAACG GGCACCTGGA CCCCAACCTT 
CCACTGCCCG GGACCCTGGC CACCACGGGG GGCATGCCCT CTGGCCCTTG CCACTCTCTG 
CAGCCGGACC AGGTGCTCAT CGCCCTGGAC TCCCGAAAGA GCAGTGTGAT GTCTCTGGGG 
AGGATGAGCT ATGACCAGCG GTCTCTGTCC AGCTCCCGCA GCTCCTATTA TGGGGCCTGG 
GGCCACAGTG GAGCCTGGGC CAGCCGCCGG TCCAGTTGGA ACAGCCTGGC CCGGGGTCAC 
AGCCTGAAGC ACCGGGCACC TTCTGCGGAA CACGAGTCAC TCCTCTCTGG GGAGAGGGGC 
CAGTCAGCAG AGGGGGAGCG GGACGACAGT GCCCCTCGTG GCCCCTCCCA TCACCTACCA 
CCCCATACCC ATCCCCATCC CCATCATCAC CACCTCCACC ACCCACACCG CTACCGGCAC 
CGGCGGACAC TCTCCCTTGA TACCAAGGAC TCTTTGGATC TGGCAGAGTT GCCCCTGCCA 
GCCCCTCATG CTCATCTGAG GTCCACCCGT AAAGTGGGCT CAGGCTTGGG TGCCAGTGAG 
CACCAGGACT GCAATGGCAA GATGCCCAAC ATTGCCAAGG ACGTTTTCAC CAAGATGGAC 
AACCGGAAGG ACCACGCGGA GGATGAGGAA GAGATTGACT ATAGCCTATG TTTCCGGATC 
CGGAAGATGA TAGATGTCTA CAAGCCTGAC TGGTGTGAAA TCAGGGAGGA CTGGTCTGTC 
TACCTCTTCT CTCCTGAGAA CAGGTTCCGG GTCCTTTGCC AAACCATCAT CACTCACAAG 
CTATTTGACT ATGTCGTCCT GGCTTTTATC TTTCTCAACT GTATCACCAT CGCCTTGGAG 
AGGCCCCAGA TAGAGGCTGG CAGCACCGAG CGTATATTCC TCACTGTGTC CAACTATATC 
TTCACCGCCA TCTTTGTTGG AGAGATGACC CTAAAGGTGG TCTCCCTGGG CCTGTATTTT 
GGGGAGCAGG CCTACCTCCG GAGCAGCTGG AACATCTTGG ATGGCTTCCT GGTCTTCGTG 
TCCCTCATTG ACATTGTGGT CTCTGTGGCT TCTGCGGGAG GAGCTACCAT ACTTGGGGTC 
CTCCGTGTCC TCCGCCTCCT CCGTACTCTT CGCCCGCTAC GGGTCATCAG CCGCGCCCCG 
GGGCTGAAGC TTGTGGTGGA GACACTGATC TCATCCCTCA AACCCATTGG AAACATCGTC 
CTCATCTGCT GCGCCTTCTT CATCATCTTC GGCATCCTGG GAGTGCAGCT CTTCAAGGGG 
AAGTTTTACC ACTGCCTGGG GGTCGACATC AGGAACATCA CCAACCGCTC CGACTGCATG 
GCCGCCAACT ACCGCTGGGT GCACCACAAG TACAACTTTG ACAACCTGGG CCAGGCCTTG 
ATGTCTCTCT TTGTCCTGGC CTCCAAAGAT GGTTGGGTCA ACATCATGTA CAACGGGCTA 
GATGCTGTGG CCGTCGACCA GCAGCCCGTG ACCAATCACA ATCCCTGGAT GTTGTTGTAC 
TTCATCTCCT TCCTGCTCAT CGTCAGCTTC TTTGTCCTTA ACATGTTTGT GGGTGTGGTG 
GTGGAGAACT TCCACAAGTG TCGGCAGCAC CAGGAGGCAG AGGAGGCGCG TCGGAGGGAG 
GAGAAGCGAC TCCGGCGGCT GGAGAAGAAG CGAAGGAAAG CCCAGCGGCT CCCATATTAT 
GCCACCTACT GCCCTACCCG GCTCCTTATT CATTCTGTCT GTACCAGTCA CTACCTGGAT 
ATCTTCATCA CCTTCATCAT CTGCCTCAAT GTCGTGACCA TGTCCTTGGA ACACTACAAC 
CAGCCCATGT CCTTGGAGAT TGCCCTCAAA TATTGCAACT ACATGTTCAC CACAGTCTTT 
GTGCTGGAAG CCATGCTGAA GCTGGTAGCA TTTGGACTGA GACGCTTCTT CAAAGACAGG 
TGGAACCAGC TGGACCTGGC CATTGTGCTC CTCTCCGTCA TGGGTATCAC CCTGGAGGAG 
ATTGAGATCA ACGCCGCCCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGGGTGCTT 
CGGATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCCACCG GGATGCGAGC CCTGCTGGAC 
ACAGTGGTTC AGGCCCTCCC CCAGGTGGGA AACCTGGGTC TTCTCTTCAT GCTTCTCTTC 
TTCATCTATG CTGCTCTGGG CGTGGAGCTC TTTGGGAAGC TGGTGTGCAG CGATGAGCAT 
CCCTGTGAGG GCATGAGCCG TCACGCCACC TTTGAAAACT TTGGTATGGC CTTCCTCACG 
CTATTCCAGG TCTCCACTGG GGACAACTGG AATGGGATTA TGAAGGACAC GCTGCGGGAC 
TGCACCCACG ACGAGCGGAG CTGCCTCAGC AACCTCCAGT TCATCTCGCC CCTGTACTTC 
GTGAGCTTCG TGCTCACCGC CCAGTTCGTG CTCATCAACG TGGTGGTGGC CGTGCTGATG 
AAGCACCTGG ACGACAGCAA CAAGGAGGCT CAGGAGGACG CCGAGATGGA CGCCGAGATC 
GAGCTGGAGA TGGCCCACGG CCTCTGCTCG CGGAGCTCCC CGGGCGCCGC GCGCCGCTGC 
GTGCGCTGCT ACTCCCCGGC ACAGGTAGGG GACCCCCGGA CCACCTGGCG CGAGCCGCCC 
GGGGGCAGGG GGACGACCGG CGCGCCTCGG GACGGGAGGG AGCGCGTAGT GGCTGGCAGG 
AACCCGGCCA GGATGGGCCG GGCTGGGCAT TGGGGCGCCG GCCGGCTGGC TCGGGGCCCA 
GGGCCCGCAA AGGTGACTGG TGAGGATAGC GGGCCCGGTG GCCGCCCGCA GGGAAGTACA 
TGGGCCCTCT GGGAGGCCCG AAGCGGAGCC TCCTGTGCTC TGAAGGAGAA CTTGTGGCTG 
GACAGTGTCT CTTTAATTAT TAAGGACTCC TTCGAAGGAG AGTTAATGAT CATCGATAAC 
CTGTCAGGCT CCGTCTTCCA CCATTATTCC TCCCCGGCCG TGTGCGAGAG GTGTGATCAT 
GACCAGCAAG AGGTCCAGCT GGCTGAGGTG GAGGCCTTCT CCCTGAACTC GGACAAGTCC 
TCTTCAATCC TGCTGGGGGA TGATGTCAGC TTGGAGGATC CCACAGCATG CCCTGTGGCC 
TCCAAGGAGA GCAAGCAAGG AGAGTCGGAG TCTCCTGACC CAATCCAGAT CGGGGACCTG 
GGAGAATGCT TCTTCCCGCT GTCCAATACG GCGGTCTCGG CAACCCCAGA GAGCTACCTG 
TGTGAGATGG AGAAGGACCC CTTCCATCCC GTCCAGTCCT GGCTGAAACA CGAGAGCAGT 
CAAACTCCCC CCAGGCCTTT CTCACCCGAT GCCGCCAACC CCCTCCTGCC CGTGCCAGCA 
GAGTTCTTCC ACCCAGCTGT GGCCACCAGC CAGAAGGGGG AGGAGAAGCC TTCCTGCAAG 
GGGACCCTCC CCAAGATCTC CCTGCAGGGC TCCTGGGCAT CCTTACGCTC TCCAAGTGTC 
AACTGTGCCC TCCTCAGACA GGTAGGGGCT GGGGGCCAGC CTGGGGTGGG GGCAGGGGGC 
TGGGGGCCAG CCTGGGTCAA GGACCAGCTC CGGCTTACAG GTGATCCTCC AGGCAGTTCA 
GGCCCCAAGG AAAGGTGCCC AGATGCCAAG GCGGCGCTCT CTCCGGCCTC CCGTCGCCGG 
AGCCTCCGAG GCCGCGGCCT CTTCAGCCTG CGGAGCCTCC GGGGCCACCA GCGCAGCCAC 
AGCAGCGGGG GCAGCACCAG TCCCGGCTGC ACCTACCATG ACTCTATGGA CCCCTCGGAC 
GAGGAGGGCA TGGGCAGCAG CCTCCGGGGC GGGGGCACCA ACAGCAGTGA GCAGTCGGAG 
ACCCTCAGCA GCCTCTCGCT CACGTCCCTC TTCTCCCCGG CCCTCCCGCC CCCGGGCCTC 
CGGCTAGCTA AGAGGTGCAA CAGCACAAGC AGCCTGTCGA CGGTGGCCCC CTTGTCCCCC 
GCCCAGGGCC CCCTGCCGCC CGTGGGGACC GAGGGCATGC AGTCCTTCTC GGTTGACCCC 
AGGGGCTTCC TGTCTGCGCC CTCCTGGGGG ACAGACTTCT GCAAGGACCG CCACTCAGCC 
AGCGAGGAGA CCCAGGGGCT GGGCGGCCGT GAGCGCAAGC AGCGCCCCGA GGTGGAGCTC 
GGGGAAGGGG TGACCAAGCG GAACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003771044.1
CDS	1..6810
Translation	MAEGVLPAAAWSPSPLPPGRSVPDLASEPGVTEQQPQPRTPPSSPAPEPGPGTDPDVPHPDLAPVVFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCEDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGRKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSLPPPDPSCWDPRVSRPHLEYDDPGVADVVEVDGALVGVDAARAAHVVIAVPVDTHAADVEVLLGRAVEGEVVLAQAALPALLLERGDFVAAHDAVVPRERADERHLVILLRLKAAVPKQAGPQLHTDYPKDEEDKETQQQYVAQHGQGVQQQVHEDAHTCGGEGEGKAMASTNHLTIACLHVSSQSVGSFFMINLCLVVIATQFSETKQREHQLMQEQRRRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRSLGLYHALRSRHQGQVEPGVGAKPEAGSAKEPRRDRLCQKHIPLDYPSHTLVQPISVTQTSDPGNCPHCQQERDQRLSLAESDDDFGREEGSANDDEGEGGGSSEGTLDLEKEEEEGEKGSGAASLCGDIWREVRAKLRGIVDSKYFNRGIMIAILVNTVSMGIEHHEQPEELTNILEISNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFDKFREGHEGHFDIKLCPIPMTPNGHLDPNLPLPGTLATTGGMPSGPCHSLQPDQVLIALDSRKSSVMSLGRMSYDQRSLSSSRSSYYGAWGHSGAWASRRSSWNSLARGHSLKHRAPSAEHESLLSGERGQSAEGERDDSAPRGPSHHLPPHTHPHPHHHHLHHPHRYRHRRTLSLDTKDSLDLAELPLPAPHAHLRSTRKVGSGLGASEHQDCNGKMPNIAKDVFTKMDNRKDHAEDEEEIDYSLCFRIRKMIDVYKPDWCEIREDWSVYLFSPENRFRVLCQTIITHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNILDGFLVFVSLIDIVVSVASAGGATILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDIRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSVCTSHYLDIFITFIICLNVVTMSLEHYNQPMSLEIALKYCNYMFTTVFVLEAMLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCSDEHPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLCSRSSPGAARRCVRCYSPAQVGDPRTTWREPPGGRGTTGAPRDGRERVVAGRNPARMGRAGHWGAGRLARGPGPAKVTGEDSGPGGRPQGSTWALWEARSGASCALKENLWLDSVSLIIKDSFEGELMIIDNLSGSVFHHYSSPAVCERCDHDQQEVQLAEVEAFSLNSDKSSSILLGDDVSLEDPTACPVASKESKQGESESPDPIQIGDLGECFFPLSNTAVSATPESYLCEMEKDPFHPVQSWLKHESSQTPPRPFSPDAANPLLPVPAEFFHPAVATSQKGEEKPSCKGTLPKISLQGSWASLRSPSVNCALLRQVGAGGQPGVGAGGWGPAWVKDQLRLTGDPPGSSGPKERCPDAKAALSPASRRRSLRGRGLFSLRSLRGHQRSHSSGGSTSPGCTYHDSMDPSDEEGMGSSLRGGGTNSSEQSETLSSLSLTSLFSPALPPPGLRLAKRCNSTSSLSTVAPLSPAQGPLPPVGTEGMQSFSVDPRGFLSAPSWGTDFCKDRHSASEETQGLGGRERKQRPEVELGEGVTKRNR

Target ORF information:

RefSeq Version	XM_003770996.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003770996.1

ORF Insert Sequence:

ATGGCCGAGG GTGTTCTGCC CGCTGCTGCC TGGAGCCCCA GCCCCCTGCC CCCCGGGCGC 
TCCGTGCCTG ATCTTGCCTC AGAGCCTGGA GTCACGGAGC AGCAGCCCCA GCCTCGGACC 
CCACCTTCCT CTCCGGCCCC GGAGCCCGGC CCCGGCACTG ACCCTGACGT CCCACACCCC 
GATCTGGCCC CCGTCGTCTT TTTTTGTCTC CGACAAACCA CCAGCCCTCG AAACTGGTGC 
ATCAAGATGG TGTGTAATCC TTGGTTCGAG TGTGTCAGCA TGTTGGTGAT TCTCCTCAAC 
TGTGTCACCC TGGGCATGTA CCAGCCATGT GAGGACATGG ATTGTCTGTC TGACCGCTGC 
AAGATCCTGC AGGTTTTTGA TGACTTCATC TTCATCTTCT TTGCCATGGA GATGGTGCTG 
AAGATGGTGG CCCTGGGGAT TTTCGGCAGG AAGTGTTACC TGGGGGACAC CTGGAACCGA 
CTGGATTTCT TCATTGTGAT GGCCGGGATG GTGGAATATT CTCTGGACCT TCAGAACATC 
AACCTGTCAG CCATCCGCAC AGTCCGAGTC CTGAGGCCCC TCAAAGCCAT CAATCGCGTG 
CCCAGCCTGC CCCCTCCGGA CCCTTCCTGC TGGGACCCCC GGGTGAGCCG GCCCCACCTG 
GAATATGACG ATCCAGGCGT AGCCGATGTT GTCGAAGTTG ATGGCGCCCT TGTGGGGGTT 
GACGCTGCCC GTGCGGCACA CGTTGTAATA GCGGTTCCAG TTGACACACA TGCCGCTGAT 
GTTGAAGTCC TGCTGGGCCG CGCTGTAGAA GGCGAAGTCG TCCTTGCTCA GGCAGCACTC 
CCGGCCCTGC TCCTTGAGCG GGGGGATTTC GTGGCAGCCC ATGATGCCGT TGTCCCCCGA 
GAGCGAGCAG ATGAACGGCA TCTCGTCATC CTCCTCCGGC TGAAAGCAGC GGTTCCGAAG 
CAGGCCGGCC CACAGCTGCA CACCGATTAT CCCAAAGATG AAGAAGACAA AGAAACACAA 
CAGCAGTACG TTGCCCAACA TGGGCAGGGT GTCCAGCAGC AGGTTCACGA GGATGCGCAT 
ACCTGTGGAG GAGAGGGTGA AGGGAAGGCT ATGGCCTCCA CAAACCACCT CACAATTGCC 
TGCTTACATG TCTCCTCGCA GAGTGTGGGC TCCTTCTTCA TGATCAACCT GTGCCTCGTT 
GTCATAGCGA CCCAGTTCTC CGAGACCAAG CAGAGAGAGC ACCAGCTGAT GCAGGAGCAG 
CGCAGGCGGT ACCTGTCCTC CAGCACCGTG GCCAGCTACG CAGAGCCCGG AGATTGCTAT 
GAGGAGATAT TCCAGTATGT CTGCCACATT CTGCGAAAAG CCAAGCGCCG AAGCCTGGGC 
CTCTACCATG CCCTCCGCAG CCGCCACCAG GGCCAGGTGG AGCCTGGTGT GGGTGCCAAG 
CCCGAGGCAG GGAGTGCCAA GGAGCCGAGG AGGGACAGGC TATGTCAGAA ACACATTCCC 
TTGGATTACC CATCCCATAC TCTCGTGCAA CCCATCTCTG TGACACAGAC CTCCGACCCT 
GGCAACTGCC CCCATTGCCA GCAGGAGAGA GATCAGCGTC TATCCCTGGC AGAGAGTGAT 
GATGACTTTG GGCGGGAGGA AGGTTCTGCC AATGATGATG AAGGCGAAGG TGGAGGAAGC 
AGTGAAGGAA CTCTGGACCT AGAGAAGGAG GAAGAGGAGG GAGAAAAAGG CAGTGGCGCA 
GCCAGCCTGT GTGGGGATAT ATGGCGGGAG GTGAGGGCCA AGCTGAGGGG CATCGTGGAC 
AGCAAATATT TTAACCGAGG CATCATGATT GCCATCTTGG TGAACACTGT CAGCATGGGC 
ATTGAACACC ACGAACAGCC CGAGGAGCTG ACCAACATCC TGGAGATCAG TAACGTGGTG 
TTCACCAGCA TGTTTGCCCT GGAGATGATC CTGAAGCTGG CGGCCTTCGG CCTCTTCGAC 
TACCTACGGA ACCCTTACAA CATCTTTGAC AGTATCATCG TCATCATCAG TATCTGGGAG 
ATCATCGGCC AGTCGGACGG GGGCCTCTCG GTACTGCGGA CGTTCCGCCT GCTTCGAGTC 
CTGAAGCTGG TCCGCTTCAT GCCAGCCCTG CGCCGCCAGC TGGTGGTTTT GATGAAGACC 
ATGGACAACG TGGCCACCTT CTGCATGCTG CTCATGCTCT TCATCTTCAT CTTCAGCATC 
CTAGGGATGC ACATTTTTGG CTGCAAATTC AGCCTCAAGA CTGACACGGG AGACACTGTA 
CCCGACCGGA AGAACTTTGA TTCGCTTCTT TGGGCCATTG TCACAGTGTT CCAGATCCTG 
ACCCAAGAGG ACTGGAATAT GGTGCTCTAC AATGGCATGG CCTCCACCTC TCCTTGGGCC 
TCCCTCTACT TTGTGGCTCT CATGACCTTC GGCAACTACG TGCTCTTCAA CCTGCTGGTG 
GCCATCCTGG TGGAGGGCTT CCAGGCAGAG GGGGATGCCA ACCGCTCCTA CTCAGATGAG 
GACCAGAGCT CGTCCAACAT GGAGGAGTTT GACAAGTTCC GAGAGGGGCA TGAAGGCCAT 
TTTGATATCA AGCTCTGCCC CATCCCCATG ACTCCCAACG GGCACCTGGA CCCCAACCTT 
CCACTGCCCG GGACCCTGGC CACCACGGGG GGCATGCCCT CTGGCCCTTG CCACTCTCTG 
CAGCCGGACC AGGTGCTCAT CGCCCTGGAC TCCCGAAAGA GCAGTGTGAT GTCTCTGGGG 
AGGATGAGCT ATGACCAGCG GTCTCTGTCC AGCTCCCGCA GCTCCTATTA TGGGGCCTGG 
GGCCACAGTG GAGCCTGGGC CAGCCGCCGG TCCAGTTGGA ACAGCCTGGC CCGGGGTCAC 
AGCCTGAAGC ACCGGGCACC TTCTGCGGAA CACGAGTCAC TCCTCTCTGG GGAGAGGGGC 
CAGTCAGCAG AGGGGGAGCG GGACGACAGT GCCCCTCGTG GCCCCTCCCA TCACCTACCA 
CCCCATACCC ATCCCCATCC CCATCATCAC CACCTCCACC ACCCACACCG CTACCGGCAC 
CGGCGGACAC TCTCCCTTGA TACCAAGGAC TCTTTGGATC TGGCAGAGTT GCCCCTGCCA 
GCCCCTCATG CTCATCTGAG GTCCACCCGT AAAGTGGGCT CAGGCTTGGG TGCCAGTGAG 
CACCAGGACT GCAATGGCAA GATGCCCAAC ATTGCCAAGG ACGTTTTCAC CAAGATGGAC 
AACCGGAAGG ACCACGCGGA GGATGAGGAA GAGATTGACT ATAGCCTATG TTTCCGGATC 
CGGAAGATGA TAGATGTCTA CAAGCCTGAC TGGTGTGAAA TCAGGGAGGA CTGGTCTGTC 
TACCTCTTCT CTCCTGAGAA CAGGTTCCGG GTCCTTTGCC AAACCATCAT CACTCACAAG 
CTATTTGACT ATGTCGTCCT GGCTTTTATC TTTCTCAACT GTATCACCAT CGCCTTGGAG 
AGGCCCCAGA TAGAGGCTGG CAGCACCGAG CGTATATTCC TCACTGTGTC CAACTATATC 
TTCACCGCCA TCTTTGTTGG AGAGATGACC CTAAAGGTGG TCTCCCTGGG CCTGTATTTT 
GGGGAGCAGG CCTACCTCCG GAGCAGCTGG AACATCTTGG ATGGCTTCCT GGTCTTCGTG 
TCCCTCATTG ACATTGTGGT CTCTGTGGCT TCTGCGGGAG GAGCTACCAT ACTTGGGGTC 
CTCCGTGTCC TCCGCCTCCT CCGTACTCTT CGCCCGCTAC GGGTCATCAG CCGCGCCCCG 
GGGCTGAAGC TTGTGGTGGA GACACTGATC TCATCCCTCA AACCCATTGG AAACATCGTC 
CTCATCTGCT GCGCCTTCTT CATCATCTTC GGCATCCTGG GAGTGCAGCT CTTCAAGGGG 
AAGTTTTACC ACTGCCTGGG GGTCGACATC AGGAACATCA CCAACCGCTC CGACTGCATG 
GCCGCCAACT ACCGCTGGGT GCACCACAAG TACAACTTTG ACAACCTGGG CCAGGCCTTG 
ATGTCTCTCT TTGTCCTGGC CTCCAAAGAT GGTTGGGTCA ACATCATGTA CAACGGGCTA 
GATGCTGTGG CCGTCGACCA GCAGCCCGTG ACCAATCACA ATCCCTGGAT GTTGTTGTAC 
TTCATCTCCT TCCTGCTCAT CGTCAGCTTC TTTGTCCTTA ACATGTTTGT GGGTGTGGTG 
GTGGAGAACT TCCACAAGTG TCGGCAGCAC CAGGAGGCAG AGGAGGCGCG TCGGAGGGAG 
GAGAAGCGAC TCCGGCGGCT GGAGAAGAAG CGAAGGAAAG CCCAGCGGCT CCCATATTAT 
GCCACCTACT GCCCTACCCG GCTCCTTATT CATTCTGTCT GTACCAGTCA CTACCTGGAT 
ATCTTCATCA CCTTCATCAT CTGCCTCAAT GTCGTGACCA TGTCCTTGGA ACACTACAAC 
CAGCCCATGT CCTTGGAGAT TGCCCTCAAA TATTGCAACT ACATGTTCAC CACAGTCTTT 
GTGCTGGAAG CCATGCTGAA GCTGGTAGCA TTTGGACTGA GACGCTTCTT CAAAGACAGG 
TGGAACCAGC TGGACCTGGC CATTGTGCTC CTCTCCGTCA TGGGTATCAC CCTGGAGGAG 
ATTGAGATCA ACGCCGCCCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGGGTGCTT 
CGGATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCCACCG GGATGCGAGC CCTGCTGGAC 
ACAGTGGTTC AGGCCCTCCC CCAGGTGGGA AACCTGGGTC TTCTCTTCAT GCTTCTCTTC 
TTCATCTATG CTGCTCTGGG CGTGGAGCTC TTTGGGAAGC TGGTGTGCAG CGATGAGCAT 
CCCTGTGAGG GCATGAGCCG TCACGCCACC TTTGAAAACT TTGGTATGGC CTTCCTCACG 
CTATTCCAGG TCTCCACTGG GGACAACTGG AATGGGATTA TGAAGGACAC GCTGCGGGAC 
TGCACCCACG ACGAGCGGAG CTGCCTCAGC AACCTCCAGT TCATCTCGCC CCTGTACTTC 
GTGAGCTTCG TGCTCACCGC CCAGTTCGTG CTCATCAACG TGGTGGTGGC CGTGCTGATG 
AAGCACCTGG ACGACAGCAA CAAGGAGGCT CAGGAGGACG CCGAGATGGA CGCCGAGATC 
GAGCTGGAGA TGGCCCACGG CCTCTGCTCG CGGAGCTCCC CGGGCGCCGC GCGCCGCTGC 
GTGCGCTGCT ACTCCCCGGC ACAGGTAGGG GACCCCCGGA CCACCTGGCG CGAGCCGCCC 
GGGGGCAGGG GGACGACCGG CGCGCCTCGG GACGGGAGGG AGCGCGTAGT GGCTGGCAGG 
AACCCGGCCA GGATGGGCCG GGCTGGGCAT TGGGGCGCCG GCCGGCTGGC TCGGGGCCCA 
GGGCCCGCAA AGGTGACTGG TGAGGATAGC GGGCCCGGTG GCCGCCCGCA GGGAAGTACA 
TGGGCCCTCT GGGAGGCCCG AAGCGGAGCC TCCTGTGCTC TGAAGGAGAA CTTGTGGCTG 
GACAGTGTCT CTTTAATTAT TAAGGACTCC TTCGAAGGAG AGTTAATGAT CATCGATAAC 
CTGTCAGGCT CCGTCTTCCA CCATTATTCC TCCCCGGCCG TGTGCGAGAG GTGTGATCAT 
GACCAGCAAG AGGTCCAGCT GGCTGAGGTG GAGGCCTTCT CCCTGAACTC GGACAAGTCC 
TCTTCAATCC TGCTGGGGGA TGATGTCAGC TTGGAGGATC CCACAGCATG CCCTGTGGCC 
TCCAAGGAGA GCAAGCAAGG AGAGTCGGAG TCTCCTGACC CAATCCAGAT CGGGGACCTG 
GGAGAATGCT TCTTCCCGCT GTCCAATACG GCGGTCTCGG CAACCCCAGA GAGCTACCTG 
TGTGAGATGG AGAAGGACCC CTTCCATCCC GTCCAGTCCT GGCTGAAACA CGAGAGCAGT 
CAAACTCCCC CCAGGCCTTT CTCACCCGAT GCCGCCAACC CCCTCCTGCC CGTGCCAGCA 
GAGTTCTTCC ACCCAGCTGT GGCCACCAGC CAGAAGGGGG AGGAGAAGCC TTCCTGCAAG 
GGGACCCTCC CCAAGATCTC CCTGCAGGGC TCCTGGGCAT CCTTACGCTC TCCAAGTGTC 
AACTGTGCCC TCCTCAGACA GGTAGGGGCT GGGGGCCAGC CTGGGGTGGG GGCAGGGGGC 
TGGGGGCCAG CCTGGGTCAA GGACCAGCTC CGGCTTACAG GTGATCCTCC AGGCAGTTCA 
GGCCCCAAGG AAAGGTGCCC AGATGCCAAG GCGGCGCTCT CTCCGGCCTC CCGTCGCCGG 
AGCCTCCGAG GCCGCGGCCT CTTCAGCCTG CGGAGCCTCC GGGGCCACCA GCGCAGCCAC 
AGCAGCGGGG GCAGCACCAG TCCCGGCTGC ACCTACCATG ACTCTATGGA CCCCTCGGAC 
GAGGAGGGCA TGGGCAGCAG CCTCCGGGGC GGGGGCACCA ACAGCAGTGA GCAGTCGGAG 
ACCCTCAGCA GCCTCTCGCT CACGTCCCTC TTCTCCCCGG CCCTCCCGCC CCCGGGCCTC 
CGGCTAGCTA AGAGGTGCAA CAGCACAAGC AGCCTGTCGA CGGTGGCCCC CTTGTCCCCC 
GCCCAGGGCC CCCTGCCGCC CGTGGGGACC GAGGGCATGC AGTCCTTCTC GGTTGACCCC 
AGGGGCTTCC TGTCTGCGCC CTCCTGGGGG ACAGACTTCT GCAAGGACCG CCACTCAGCC 
AGCGAGGAGA CCCAGGGGCT GGGCGGCCGT GAGCGCAAGC AGCGCCCCGA GGTGGAGCTC 
GGGGAAGGGG TGACCAAGCG GAACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_023504351.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023360119.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.

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CloneID	OSa155935
Clone ID Related Accession (Same CDS sequence)	XM_023504351.1
Accession Version	XM_023504351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843601.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 14% of CDS bases ##RefSeq-Attributes-END##

ATGGCCGAGG GTGTTCTGCC CGCTGCTGCC TGGAGCCCCA GCCCCCTGCC CCCCGGGCGC 
TCCGTGCCTG ATCTTGCCTC AGAGCCTGGA GTCACGGAGC AGCAGCCCCA GCCTCGGACC 
CCACCTTCCT CTCCGGCCCC GGAGCCCGGC CCCGGCACTG ACCCTGACGT CCCACACCCC 
GATCTGGCCC CCGTCGTCTT TTTTTGTCTC CGACAAACCA CCAGCCCTCG AAACTGGTGC 
ATCAAGATGG TGTGTAATCC TTGGTTCGAG TGTGTCAGCA TGTTGGTGAT TCTCCTCAAC 
TGTGTCACCC TGGGCATGTA CCAGCCATGT GAGGACATGG ATTGTCTGTC TGACCGCTGC 
AAGATCCTGC AGGTTTTTGA TGACTTCATC TTCATCTTCT TTGCCATGGA GATGGTGCTG 
AAGATGGTGG CCCTGGGGAT TTTCGGCAGG AAGTGTTACC TGGGGGACAC CTGGAACCGA 
CTGGATTTCT TCATTGTGAT GGCCGGGATG GTGGAATATT CTCTGGACCT TCAGAACATC 
AACCTGTCAG CCATCCGCAC AGTCCGAGTC CTGAGGCCCC TCAAAGCCAT CAATCGCGTG 
CCCAGTAAGC AATTACTTTC CACTCTGAGC CTTAATTTCC TTCCTGCCAT CCATGGTTGC 
CTGATGCTGA TGGTTGGCCT GGTGCTGAAG GACAGCTCCT CTCCAAGATG CAGCAGAGCC 
CGGCCCCTGG GCCTTTGTGT GCCCTCTCTG GCCGAGCGCT GTGCACCCCG TTGGCAGGAG 
CCACCGAACT CCTCTCTGCG CTATCTCTGC CCTCATCCCA GCCAAGGGAG CGCCAGCCTG 
GGCCTGCCCC CTCCGGACCC TTCCTGCTGG GACCCCCGGG TGAGCCGGCC CCACCTGGAA 
TATGACGATC CAGGCGTAGC CGATGTTGTC GAAGTTGATG GCGCCCTTGT GGGGGTTGAC 
GCTGCCCGTG CGGCACACGT TGTAATAGCG GTTCCAGTTG ACACACATGC CGCTGATGTT 
GAAGTCCTGC TGGGCCGCGC TGTAGAAGGC GAAGTCGTCC TTGCTCAGGC AGCACTCCCG 
GCCCTGCTCC TTGAGCGGGG GGATTTCGTG GCAGCCCATG ATGCCGTTGT CCCCCGAGAG 
CGAGCAGATG AACGGCATCT CGTCATCCTC CTCCGGCTGA AAGCAGCGGT TCCGAAGCAG 
GCCGGCCCAC AGCTGCACAC CGATTATCCC AAAGATGAAG AAGACAAAGA AACACAACAG 
CAGTACGTTG CCCAACATGG GCAGGGTGTC CAGCAGCAGG TTCACGAGGA TGCGCATACC 
TGTGGAGGAG AGGGTGAAGG GAAGGCTATG GCCTCCACAA ACCACCTCAC AATTGCCTGC 
TTACATGTCT CCTCGCAGAG TGTGGGCTCC TTCTTCATGA TCAACCTGTG CCTCGTTGTC 
ATAGCGACCC AGTTCTCCGA GACCAAGCAG AGAGAGCACC AGCTGATGCA GGAGCAGCGC 
AGGCGGTACC TGTCCTCCAG CACCGTGGCC AGCTACGCAG AGCCCGGAGA TTGCTATGAG 
GAGATATTCC AGTATGTCTG CCACATTCTG CGAAAAGCCA AGCGCCGAAG CCTGGGCCTC 
TACCATGCCC TCCGCAGCCG CCACCAGGGC CAGGTGGAGC CTGGTGTGGG TGCCAAGCCC 
GAGGCAGGGA GTGCCAAGGA GCCGAGGAGG GACAGGCTAT GTCAGAAACA CATTCCCTTG 
GATTACCCAT CCCATACTCT CGTGCAACCC ATCTCTGTGA CACAGACCTC CGACCCTGGC 
AACTGCCCCC ATTGCCAGCA GGAGAGAGAT CAGCGTCTAT CCCTGGCAGA GAGTGATGAT 
GACTTTGGGC GGGAGGAAGG TTCTGCCAAT GATGATGAAG GCGAAGGTGG AGGAAGCAGT 
GAAGGAACTC TGGACCTAGA GAAGGAGGAA GAGGAGGGAG AAAAAGGCAG TGGCGCAGCC 
AGCCTGTGTG GGGATATATG GCGGGAGGTG AGGGCCAAGC TGAGGGGCAT CGTGGACAGC 
AAATATTTTA ACCGAGGCAT CATGATTGCC ATCTTGGTGA ACACTGTCAG CATGGGCATT 
GAACACCACG AACAGCCCGA GGAGCTGACC AACATCCTGG AGATCAGTAA CGTGGTGTTC 
ACCAGCATGT TTGCCCTGGA GATGATCCTG AAGCTGGCGG CCTTCGGCCT CTTCGACTAC 
CTACGGAACC CTTACAACAT CTTTGACAGT ATCATCGTCA TCATCAGTAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTCTCGGTA CTGCGGACGT TCCGCCTGCT TCGAGTCCTG 
AAGCTGGTCC GCTTCATGCC AGCCCTGCGC CGCCAGCTGG TGGTTTTGAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTA 
GGGATGCACA TTTTTGGCTG CAAATTCAGC CTCAAGACTG ACACGGGAGA CACTGTACCC 
GACCGGAAGA ACTTTGATTC GCTTCTTTGG GCCATTGTCA CAGTGTTCCA GATCCTGACC 
CAAGAGGACT GGAATATGGT GCTCTACAAT GGCATGGCCT CCACCTCTCC TTGGGCCTCC 
CTCTACTTTG TGGCTCTCAT GACCTTCGGC AACTACGTGC TCTTCAACCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGG GATGCCAACC GCTCCTACTC AGATGAGGAC 
CAGAGCTCGT CCAACATGGA GGAGTTTGAC AAGTTCCGAG AGGGGCATGA AGGCCATTTT 
GATATCAAGC TCTGCCCCAT CCCCATGACT CCCAACGGGC ACCTGGACCC CAACCTTCCA 
CTGCCCGGGA CCCTGGCCAC CACGGGGGGC ATGCCCTCTG GCCCTTGCCA CTCTCTGCAG 
CCGGACCAGG TGCTCATCGC CCTGGACTCC CGAAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTATG ACCAGCGGTC TCTGTCCAGC TCCCGCAGCT CCTATTATGG GGCCTGGGGC 
CACAGTGGAG CCTGGGCCAG CCGCCGGTCC AGTTGGAACA GCCTGGCCCG GGGTCACAGC 
CTGAAGCACC GGGCACCTTC TGCGGAACAC GAGTCACTCC TCTCTGGGGA GAGGGGCCAG 
TCAGCAGAGG GGGAGCGGGA CGACAGTGCC CCTCGTGGCC CCTCCCATCA CCTACCACCC 
CATACCCATC CCCATCCCCA TCATCACCAC CTCCACCACC CACACCGCTA CCGGCACCGG 
CGGACACTCT CCCTTGATAC CAAGGACTCT TTGGATCTGG CAGAGTTGCC CCTGCCAGCC 
CCTCATGCTC ATCTGAGGTC CACCCGTAAA GTGGGCTCAG GCTTGGGTGC CAGTGAGCAC 
CAGGACTGCA ATGGCAAGAT GCCCAACATT GCCAAGGACG TTTTCACCAA GATGGACAAC 
CGGAAGGACC ACGCGGAGGA TGAGGAAGAG ATTGACTATA GCCTATGTTT CCGGATCCGG 
AAGATGATAG ATGTCTACAA GCCTGACTGG TGTGAAATCA GGGAGGACTG GTCTGTCTAC 
CTCTTCTCTC CTGAGAACAG GTTCCGGGTC CTTTGCCAAA CCATCATCAC TCACAAGCTA 
TTTGACTATG TCGTCCTGGC TTTTATCTTT CTCAACTGTA TCACCATCGC CTTGGAGAGG 
CCCCAGATAG AGGCTGGCAG CACCGAGCGT ATATTCCTCA CTGTGTCCAA CTATATCTTC 
ACCGCCATCT TTGTTGGAGA GATGACCCTA AAGGTGGTCT CCCTGGGCCT GTATTTTGGG 
GAGCAGGCCT ACCTCCGGAG CAGCTGGAAC ATCTTGGATG GCTTCCTGGT CTTCGTGTCC 
CTCATTGACA TTGTGGTCTC TGTGGCTTCT GCGGGAGGAG CTACCATACT TGGGGTCCTC 
CGTGTCCTCC GCCTCCTCCG TACTCTTCGC CCGCTACGGG TCATCAGCCG CGCCCCGGGG 
CTGAAGCTTG TGGTGGAGAC ACTGATCTCA TCCCTCAAAC CCATTGGAAA CATCGTCCTC 
ATCTGCTGCG CCTTCTTCAT CATCTTCGGC ATCCTGGGAG TGCAGCTCTT CAAGGGGAAG 
TTTTACCACT GCCTGGGGGT CGACATCAGG AACATCACCA ACCGCTCCGA CTGCATGGCC 
GCCAACTACC GCTGGGTGCA CCACAAGTAC AACTTTGACA ACCTGGGCCA GGCCTTGATG 
TCTCTCTTTG TCCTGGCCTC CAAAGATGGT TGGGTCAACA TCATGTACAA CGGGCTAGAT 
GCTGTGGCCG TCGACCAGCA GCCCGTGACC AATCACAATC CCTGGATGTT GTTGTACTTC 
ATCTCCTTCC TGCTCATCGT CAGCTTCTTT GTCCTTAACA TGTTTGTGGG TGTGGTGGTG 
GAGAACTTCC ACAAGTGTCG GCAGCACCAG GAGGCAGAGG AGGCGCGTCG GAGGGAGGAG 
AAGCGACTCC GGCGGCTGGA GAAGAAGCGA AGGAAAGCCC AGCGGCTCCC ATATTATGCC 
ACCTACTGCC CTACCCGGCT CCTTATTCAT TCTGTCTGTA CCAGTCACTA CCTGGATATC 
TTCATCACCT TCATCATCTG CCTCAATGTC GTGACCATGT CCTTGGAACA CTACAACCAG 
CCCATGTCCT TGGAGATTGC CCTCAAATAT TGCAACTACA TGTTCACCAC AGTCTTTGTG 
CTGGAAGCCA TGCTGAAGCT GGTAGCATTT GGACTGAGAC GCTTCTTCAA AGACAGGTGG 
AACCAGCTGG ACCTGGCCAT TGTGCTCCTC TCCGTCATGG GTATCACCCT GGAGGAGATT 
GAGATCAACG CCGCCCTGCC CATCAACCCC ACCATCATCC GCATCATGAG GGTGCTTCGG 
ATTGCCCGAG TGCTGAAGCT GCTGAAGATG GCCACCGGGA TGCGAGCCCT GCTGGACACA 
GTGGTTCAGG CCCTCCCCCA GGTGGGAAAC CTGGGTCTTC TCTTCATGCT TCTCTTCTTC 
ATCTATGCTG CTCTGGGCGT GGAGCTCTTT GGGAAGCTGG TGTGCAGCGA TGAGCATCCC 
TGTGAGGGCA TGAGCCGTCA CGCCACCTTT GAAAACTTTG GTATGGCCTT CCTCACGCTA 
TTCCAGGTCT CCACTGGGGA CAACTGGAAT GGGATTATGA AGGACACGCT GCGGGACTGC 
ACCCACGACG AGCGGAGCTG CCTCAGCAAC CTCCAGTTCA TCTCGCCCCT GTACTTCGTG 
AGCTTCGTGC TCACCGCCCA GTTCGTGCTC ATCAACGTGG TGGTGGCCGT GCTGATGAAG 
CACCTGGACG ACAGCAACAA GGAGGCTCAG GAGGACGCCG AGATGGACGC CGAGATCGAG 
CTGGAGATGG CCCACGGCCT CTGCTCGCGG AGCTCCCCGG GCGCCGCGCG CCGCTGCGTG 
CGCTGCTACT CCCCGGCACA GGAGAACTTG TGGCTGGACA GTGTCTCTTT AATTATTAAG 
GACTCCTTCG AAGGAGAGTT AATGATCATC GATAACCTGT CAGGCTCCGT CTTCCACCAT 
TATTCCTCCC CGGCCGTGTG CGAGAGGTGT GATCATGACC AGCAAGAGGT CCAGCTGGCT 
GAGGTGGAGG CCTTCTCCCT GAACTCGGAC AAGTCCTCTT CAATCCTGCT GGGGGATGAT 
GTCAGCTTGG AGGATCCCAC AGCATGCCCT GTGGCCTCCA AGGAGAGCAA GCAAGGAGAG 
TCGGAGTCTC CTGACCCAAT CCAGATCGGG GACCTGGGAG AATGCTTCTT CCCGCTGTCC 
AATACGGCGG TCTCGGCAAC CCCAGAGAGC TACCTGTGTG AGATGGAGAA GGACCCCTTC 
CATCCCGTCC AGTCCTGGCT GAAACACGAG AGCAGTCAAA CTCCCCCCAG GCCTTTCTCA 
CCCGATGCCG CCAACCCCCT CCTGCCCGTG CCAGCAGAGT TCTTCCACCC AGCTGTGGCC 
ACCAGCCAGA AGGGGGAGGA GAAGCCTTCC TGCAAGGGGA CCCTCCCCAA GATCTCCCTG 
CAGGGCTCCT GGGCATCCTT ACGCTCTCCA AGTGTCAACT GTGCCCTCCT CAGACAGGTA 
GGGGCTGGGG GCCAGCCTGG GGTGGGGGCA GGGGGCTGGG GGCCAGCCTG GGTCAAGGAC 
CAGCTCCGGC TTACAGGTGA TCCTCCAGGC AGTTCAGGCC CCAAGGAAAG GTGCCCAGAT 
GCCAAGGCGG CGCTCTCTCC GGCCTCCCGT CGCCGGAGCC TCCGAGGCCG CGGCCTCTTC 
AGCCTGCGGA GCCTCCGGGG CCACCAGCGC AGCCACAGCA GCGGGGGCAG CACCAGTCCC 
GGCTGCACCT ACCATGACTC TATGGACCCC TCGGACGAGG AGGGCATGGG CAGCAGCCTC 
CGGGGCGGGG GCACCAACAG CAGTGAGCAG TCGGAGACCC TCAGCAGCCT CTCGCTCACG 
TCCCTCTTCT CCCCGGCCCT CCCGCCCCCG GGCCTCCGGC TAGCTAAGAG GTGCAACAGC 
ACAAGCAGCC TGTCGACGGT GGCCCCCTTG TCCCCCGCCC AGGGCCCCCT GCCGCCCGTG 
GGGACCGAGG GCATGCAGTC CTTCTCGGTT GACCCCAGGG GCTTCCTGTC TGCGCCCTCC 
TGGGGGACAG ACTTCTGCAA GGACCGCCAC TCAGCCAGCG AGGAGACCCA GGGGCTGGGC 
GGCCGTGAGC GCAAGCAGCG CCCCGAGGTG GAGCTCGGGG AAGGGGTGAC CAAGCGGAAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023360119.1
CDS	1..6786
Translation	MAEGVLPAAAWSPSPLPPGRSVPDLASEPGVTEQQPQPRTPPSSPAPEPGPGTDPDVPHPDLAPVVFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCEDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGRKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSKQLLSTLSLNFLPAIHGCLMLMVGLVLKDSSSPRCSRARPLGLCVPSLAERCAPRWQEPPNSSLRYLCPHPSQGSASLGLPPPDPSCWDPRVSRPHLEYDDPGVADVVEVDGALVGVDAARAAHVVIAVPVDTHAADVEVLLGRAVEGEVVLAQAALPALLLERGDFVAAHDAVVPRERADERHLVILLRLKAAVPKQAGPQLHTDYPKDEEDKETQQQYVAQHGQGVQQQVHEDAHTCGGEGEGKAMASTNHLTIACLHVSSQSVGSFFMINLCLVVIATQFSETKQREHQLMQEQRRRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRSLGLYHALRSRHQGQVEPGVGAKPEAGSAKEPRRDRLCQKHIPLDYPSHTLVQPISVTQTSDPGNCPHCQQERDQRLSLAESDDDFGREEGSANDDEGEGGGSSEGTLDLEKEEEEGEKGSGAASLCGDIWREVRAKLRGIVDSKYFNRGIMIAILVNTVSMGIEHHEQPEELTNILEISNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFDKFREGHEGHFDIKLCPIPMTPNGHLDPNLPLPGTLATTGGMPSGPCHSLQPDQVLIALDSRKSSVMSLGRMSYDQRSLSSSRSSYYGAWGHSGAWASRRSSWNSLARGHSLKHRAPSAEHESLLSGERGQSAEGERDDSAPRGPSHHLPPHTHPHPHHHHLHHPHRYRHRRTLSLDTKDSLDLAELPLPAPHAHLRSTRKVGSGLGASEHQDCNGKMPNIAKDVFTKMDNRKDHAEDEEEIDYSLCFRIRKMIDVYKPDWCEIREDWSVYLFSPENRFRVLCQTIITHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNILDGFLVFVSLIDIVVSVASAGGATILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDIRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSVCTSHYLDIFITFIICLNVVTMSLEHYNQPMSLEIALKYCNYMFTTVFVLEAMLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCSDEHPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLCSRSSPGAARRCVRCYSPAQENLWLDSVSLIIKDSFEGELMIIDNLSGSVFHHYSSPAVCERCDHDQQEVQLAEVEAFSLNSDKSSSILLGDDVSLEDPTACPVASKESKQGESESPDPIQIGDLGECFFPLSNTAVSATPESYLCEMEKDPFHPVQSWLKHESSQTPPRPFSPDAANPLLPVPAEFFHPAVATSQKGEEKPSCKGTLPKISLQGSWASLRSPSVNCALLRQVGAGGQPGVGAGGWGPAWVKDQLRLTGDPPGSSGPKERCPDAKAALSPASRRRSLRGRGLFSLRSLRGHQRSHSSGGSTSPGCTYHDSMDPSDEEGMGSSLRGGGTNSSEQSETLSSLSLTSLFSPALPPPGLRLAKRCNSTSSLSTVAPLSPAQGPLPPVGTEGMQSFSVDPRGFLSAPSWGTDFCKDRHSASEETQGLGGRERKQRPEVELGEGVTKRNR

Target ORF information:

RefSeq Version	XM_023504351.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023504351.1

ORF Insert Sequence:

ATGGCCGAGG GTGTTCTGCC CGCTGCTGCC TGGAGCCCCA GCCCCCTGCC CCCCGGGCGC 
TCCGTGCCTG ATCTTGCCTC AGAGCCTGGA GTCACGGAGC AGCAGCCCCA GCCTCGGACC 
CCACCTTCCT CTCCGGCCCC GGAGCCCGGC CCCGGCACTG ACCCTGACGT CCCACACCCC 
GATCTGGCCC CCGTCGTCTT TTTTTGTCTC CGACAAACCA CCAGCCCTCG AAACTGGTGC 
ATCAAGATGG TGTGTAATCC TTGGTTCGAG TGTGTCAGCA TGTTGGTGAT TCTCCTCAAC 
TGTGTCACCC TGGGCATGTA CCAGCCATGT GAGGACATGG ATTGTCTGTC TGACCGCTGC 
AAGATCCTGC AGGTTTTTGA TGACTTCATC TTCATCTTCT TTGCCATGGA GATGGTGCTG 
AAGATGGTGG CCCTGGGGAT TTTCGGCAGG AAGTGTTACC TGGGGGACAC CTGGAACCGA 
CTGGATTTCT TCATTGTGAT GGCCGGGATG GTGGAATATT CTCTGGACCT TCAGAACATC 
AACCTGTCAG CCATCCGCAC AGTCCGAGTC CTGAGGCCCC TCAAAGCCAT CAATCGCGTG 
CCCAGTAAGC AATTACTTTC CACTCTGAGC CTTAATTTCC TTCCTGCCAT CCATGGTTGC 
CTGATGCTGA TGGTTGGCCT GGTGCTGAAG GACAGCTCCT CTCCAAGATG CAGCAGAGCC 
CGGCCCCTGG GCCTTTGTGT GCCCTCTCTG GCCGAGCGCT GTGCACCCCG TTGGCAGGAG 
CCACCGAACT CCTCTCTGCG CTATCTCTGC CCTCATCCCA GCCAAGGGAG CGCCAGCCTG 
GGCCTGCCCC CTCCGGACCC TTCCTGCTGG GACCCCCGGG TGAGCCGGCC CCACCTGGAA 
TATGACGATC CAGGCGTAGC CGATGTTGTC GAAGTTGATG GCGCCCTTGT GGGGGTTGAC 
GCTGCCCGTG CGGCACACGT TGTAATAGCG GTTCCAGTTG ACACACATGC CGCTGATGTT 
GAAGTCCTGC TGGGCCGCGC TGTAGAAGGC GAAGTCGTCC TTGCTCAGGC AGCACTCCCG 
GCCCTGCTCC TTGAGCGGGG GGATTTCGTG GCAGCCCATG ATGCCGTTGT CCCCCGAGAG 
CGAGCAGATG AACGGCATCT CGTCATCCTC CTCCGGCTGA AAGCAGCGGT TCCGAAGCAG 
GCCGGCCCAC AGCTGCACAC CGATTATCCC AAAGATGAAG AAGACAAAGA AACACAACAG 
CAGTACGTTG CCCAACATGG GCAGGGTGTC CAGCAGCAGG TTCACGAGGA TGCGCATACC 
TGTGGAGGAG AGGGTGAAGG GAAGGCTATG GCCTCCACAA ACCACCTCAC AATTGCCTGC 
TTACATGTCT CCTCGCAGAG TGTGGGCTCC TTCTTCATGA TCAACCTGTG CCTCGTTGTC 
ATAGCGACCC AGTTCTCCGA GACCAAGCAG AGAGAGCACC AGCTGATGCA GGAGCAGCGC 
AGGCGGTACC TGTCCTCCAG CACCGTGGCC AGCTACGCAG AGCCCGGAGA TTGCTATGAG 
GAGATATTCC AGTATGTCTG CCACATTCTG CGAAAAGCCA AGCGCCGAAG CCTGGGCCTC 
TACCATGCCC TCCGCAGCCG CCACCAGGGC CAGGTGGAGC CTGGTGTGGG TGCCAAGCCC 
GAGGCAGGGA GTGCCAAGGA GCCGAGGAGG GACAGGCTAT GTCAGAAACA CATTCCCTTG 
GATTACCCAT CCCATACTCT CGTGCAACCC ATCTCTGTGA CACAGACCTC CGACCCTGGC 
AACTGCCCCC ATTGCCAGCA GGAGAGAGAT CAGCGTCTAT CCCTGGCAGA GAGTGATGAT 
GACTTTGGGC GGGAGGAAGG TTCTGCCAAT GATGATGAAG GCGAAGGTGG AGGAAGCAGT 
GAAGGAACTC TGGACCTAGA GAAGGAGGAA GAGGAGGGAG AAAAAGGCAG TGGCGCAGCC 
AGCCTGTGTG GGGATATATG GCGGGAGGTG AGGGCCAAGC TGAGGGGCAT CGTGGACAGC 
AAATATTTTA ACCGAGGCAT CATGATTGCC ATCTTGGTGA ACACTGTCAG CATGGGCATT 
GAACACCACG AACAGCCCGA GGAGCTGACC AACATCCTGG AGATCAGTAA CGTGGTGTTC 
ACCAGCATGT TTGCCCTGGA GATGATCCTG AAGCTGGCGG CCTTCGGCCT CTTCGACTAC 
CTACGGAACC CTTACAACAT CTTTGACAGT ATCATCGTCA TCATCAGTAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTCTCGGTA CTGCGGACGT TCCGCCTGCT TCGAGTCCTG 
AAGCTGGTCC GCTTCATGCC AGCCCTGCGC CGCCAGCTGG TGGTTTTGAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTA 
GGGATGCACA TTTTTGGCTG CAAATTCAGC CTCAAGACTG ACACGGGAGA CACTGTACCC 
GACCGGAAGA ACTTTGATTC GCTTCTTTGG GCCATTGTCA CAGTGTTCCA GATCCTGACC 
CAAGAGGACT GGAATATGGT GCTCTACAAT GGCATGGCCT CCACCTCTCC TTGGGCCTCC 
CTCTACTTTG TGGCTCTCAT GACCTTCGGC AACTACGTGC TCTTCAACCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGG GATGCCAACC GCTCCTACTC AGATGAGGAC 
CAGAGCTCGT CCAACATGGA GGAGTTTGAC AAGTTCCGAG AGGGGCATGA AGGCCATTTT 
GATATCAAGC TCTGCCCCAT CCCCATGACT CCCAACGGGC ACCTGGACCC CAACCTTCCA 
CTGCCCGGGA CCCTGGCCAC CACGGGGGGC ATGCCCTCTG GCCCTTGCCA CTCTCTGCAG 
CCGGACCAGG TGCTCATCGC CCTGGACTCC CGAAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTATG ACCAGCGGTC TCTGTCCAGC TCCCGCAGCT CCTATTATGG GGCCTGGGGC 
CACAGTGGAG CCTGGGCCAG CCGCCGGTCC AGTTGGAACA GCCTGGCCCG GGGTCACAGC 
CTGAAGCACC GGGCACCTTC TGCGGAACAC GAGTCACTCC TCTCTGGGGA GAGGGGCCAG 
TCAGCAGAGG GGGAGCGGGA CGACAGTGCC CCTCGTGGCC CCTCCCATCA CCTACCACCC 
CATACCCATC CCCATCCCCA TCATCACCAC CTCCACCACC CACACCGCTA CCGGCACCGG 
CGGACACTCT CCCTTGATAC CAAGGACTCT TTGGATCTGG CAGAGTTGCC CCTGCCAGCC 
CCTCATGCTC ATCTGAGGTC CACCCGTAAA GTGGGCTCAG GCTTGGGTGC CAGTGAGCAC 
CAGGACTGCA ATGGCAAGAT GCCCAACATT GCCAAGGACG TTTTCACCAA GATGGACAAC 
CGGAAGGACC ACGCGGAGGA TGAGGAAGAG ATTGACTATA GCCTATGTTT CCGGATCCGG 
AAGATGATAG ATGTCTACAA GCCTGACTGG TGTGAAATCA GGGAGGACTG GTCTGTCTAC 
CTCTTCTCTC CTGAGAACAG GTTCCGGGTC CTTTGCCAAA CCATCATCAC TCACAAGCTA 
TTTGACTATG TCGTCCTGGC TTTTATCTTT CTCAACTGTA TCACCATCGC CTTGGAGAGG 
CCCCAGATAG AGGCTGGCAG CACCGAGCGT ATATTCCTCA CTGTGTCCAA CTATATCTTC 
ACCGCCATCT TTGTTGGAGA GATGACCCTA AAGGTGGTCT CCCTGGGCCT GTATTTTGGG 
GAGCAGGCCT ACCTCCGGAG CAGCTGGAAC ATCTTGGATG GCTTCCTGGT CTTCGTGTCC 
CTCATTGACA TTGTGGTCTC TGTGGCTTCT GCGGGAGGAG CTACCATACT TGGGGTCCTC 
CGTGTCCTCC GCCTCCTCCG TACTCTTCGC CCGCTACGGG TCATCAGCCG CGCCCCGGGG 
CTGAAGCTTG TGGTGGAGAC ACTGATCTCA TCCCTCAAAC CCATTGGAAA CATCGTCCTC 
ATCTGCTGCG CCTTCTTCAT CATCTTCGGC ATCCTGGGAG TGCAGCTCTT CAAGGGGAAG 
TTTTACCACT GCCTGGGGGT CGACATCAGG AACATCACCA ACCGCTCCGA CTGCATGGCC 
GCCAACTACC GCTGGGTGCA CCACAAGTAC AACTTTGACA ACCTGGGCCA GGCCTTGATG 
TCTCTCTTTG TCCTGGCCTC CAAAGATGGT TGGGTCAACA TCATGTACAA CGGGCTAGAT 
GCTGTGGCCG TCGACCAGCA GCCCGTGACC AATCACAATC CCTGGATGTT GTTGTACTTC 
ATCTCCTTCC TGCTCATCGT CAGCTTCTTT GTCCTTAACA TGTTTGTGGG TGTGGTGGTG 
GAGAACTTCC ACAAGTGTCG GCAGCACCAG GAGGCAGAGG AGGCGCGTCG GAGGGAGGAG 
AAGCGACTCC GGCGGCTGGA GAAGAAGCGA AGGAAAGCCC AGCGGCTCCC ATATTATGCC 
ACCTACTGCC CTACCCGGCT CCTTATTCAT TCTGTCTGTA CCAGTCACTA CCTGGATATC 
TTCATCACCT TCATCATCTG CCTCAATGTC GTGACCATGT CCTTGGAACA CTACAACCAG 
CCCATGTCCT TGGAGATTGC CCTCAAATAT TGCAACTACA TGTTCACCAC AGTCTTTGTG 
CTGGAAGCCA TGCTGAAGCT GGTAGCATTT GGACTGAGAC GCTTCTTCAA AGACAGGTGG 
AACCAGCTGG ACCTGGCCAT TGTGCTCCTC TCCGTCATGG GTATCACCCT GGAGGAGATT 
GAGATCAACG CCGCCCTGCC CATCAACCCC ACCATCATCC GCATCATGAG GGTGCTTCGG 
ATTGCCCGAG TGCTGAAGCT GCTGAAGATG GCCACCGGGA TGCGAGCCCT GCTGGACACA 
GTGGTTCAGG CCCTCCCCCA GGTGGGAAAC CTGGGTCTTC TCTTCATGCT TCTCTTCTTC 
ATCTATGCTG CTCTGGGCGT GGAGCTCTTT GGGAAGCTGG TGTGCAGCGA TGAGCATCCC 
TGTGAGGGCA TGAGCCGTCA CGCCACCTTT GAAAACTTTG GTATGGCCTT CCTCACGCTA 
TTCCAGGTCT CCACTGGGGA CAACTGGAAT GGGATTATGA AGGACACGCT GCGGGACTGC 
ACCCACGACG AGCGGAGCTG CCTCAGCAAC CTCCAGTTCA TCTCGCCCCT GTACTTCGTG 
AGCTTCGTGC TCACCGCCCA GTTCGTGCTC ATCAACGTGG TGGTGGCCGT GCTGATGAAG 
CACCTGGACG ACAGCAACAA GGAGGCTCAG GAGGACGCCG AGATGGACGC CGAGATCGAG 
CTGGAGATGG CCCACGGCCT CTGCTCGCGG AGCTCCCCGG GCGCCGCGCG CCGCTGCGTG 
CGCTGCTACT CCCCGGCACA GGAGAACTTG TGGCTGGACA GTGTCTCTTT AATTATTAAG 
GACTCCTTCG AAGGAGAGTT AATGATCATC GATAACCTGT CAGGCTCCGT CTTCCACCAT 
TATTCCTCCC CGGCCGTGTG CGAGAGGTGT GATCATGACC AGCAAGAGGT CCAGCTGGCT 
GAGGTGGAGG CCTTCTCCCT GAACTCGGAC AAGTCCTCTT CAATCCTGCT GGGGGATGAT 
GTCAGCTTGG AGGATCCCAC AGCATGCCCT GTGGCCTCCA AGGAGAGCAA GCAAGGAGAG 
TCGGAGTCTC CTGACCCAAT CCAGATCGGG GACCTGGGAG AATGCTTCTT CCCGCTGTCC 
AATACGGCGG TCTCGGCAAC CCCAGAGAGC TACCTGTGTG AGATGGAGAA GGACCCCTTC 
CATCCCGTCC AGTCCTGGCT GAAACACGAG AGCAGTCAAA CTCCCCCCAG GCCTTTCTCA 
CCCGATGCCG CCAACCCCCT CCTGCCCGTG CCAGCAGAGT TCTTCCACCC AGCTGTGGCC 
ACCAGCCAGA AGGGGGAGGA GAAGCCTTCC TGCAAGGGGA CCCTCCCCAA GATCTCCCTG 
CAGGGCTCCT GGGCATCCTT ACGCTCTCCA AGTGTCAACT GTGCCCTCCT CAGACAGGTA 
GGGGCTGGGG GCCAGCCTGG GGTGGGGGCA GGGGGCTGGG GGCCAGCCTG GGTCAAGGAC 
CAGCTCCGGC TTACAGGTGA TCCTCCAGGC AGTTCAGGCC CCAAGGAAAG GTGCCCAGAT 
GCCAAGGCGG CGCTCTCTCC GGCCTCCCGT CGCCGGAGCC TCCGAGGCCG CGGCCTCTTC 
AGCCTGCGGA GCCTCCGGGG CCACCAGCGC AGCCACAGCA GCGGGGGCAG CACCAGTCCC 
GGCTGCACCT ACCATGACTC TATGGACCCC TCGGACGAGG AGGGCATGGG CAGCAGCCTC 
CGGGGCGGGG GCACCAACAG CAGTGAGCAG TCGGAGACCC TCAGCAGCCT CTCGCTCACG 
TCCCTCTTCT CCCCGGCCCT CCCGCCCCCG GGCCTCCGGC TAGCTAAGAG GTGCAACAGC 
ACAAGCAGCC TGTCGACGGT GGCCCCCTTG TCCCCCGCCC AGGGCCCCCT GCCGCCCGTG 
GGGACCGAGG GCATGCAGTC CTTCTCGGTT GACCCCAGGG GCTTCCTGTC TGCGCCCTCC 
TGGGGGACAG ACTTCTGCAA GGACCGCCAC TCAGCCAGCG AGGAGACCCA GGGGCTGGGC 
GGCCGTGAGC GCAAGCAGCG CCCCGAGGTG GAGCTCGGGG AAGGGGTGAC CAAGCGGAAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_031940123.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795983.1 Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.

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CloneID	OSa238048
Clone ID Related Accession (Same CDS sequence)	XM_031940123.1
Accession Version	XM_031940123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTTGGTGA TTCTCCTCAA CTGTGTCACC CTGGGCATGT ACCAGCCATG TGAGGACATG 
GATTGTCTGT CTGACCGCTG CAAGATCCTG CAGGTTTTTG ATGACTTCAT CTTCATCTTC 
TTTGCCATGG AGATGGTGCT GAAGATGGTG GCCCTGGGGA TTTTCGGCAG GAAGTGTTAC 
CTGGGGGACA CCTGGAACCG ACTGGATTTC TTCATTGTGA TGGCCGGGAT GGTGGAATAT 
TCTCTGGACC TTCAGAACAT CAACCTGTCA GCCATCCGCA CAGTCCGAGT CCTGAGGCCC 
CTCAAAGCCA TCAATCGCGT GCCCAGTATG CGCATCCTCG TGAACCTGCT GCTGGACACC 
CTGCCCATGT TGGGCAACGT ACTGCTGTTG TGTTTCTTTG TCTTCTTCAT CTTTGGGATA 
ATCGGTGTGC AGCTGTGGGC CGGCCTGCTT CGGAACCGCT GCTTTCTGGA GGACAACTTC 
ACCATACAAG GGGATGTGGC CCTCCCTCCC TACTACCAGC CGGAGGAGGA TGACGAGATG 
CCGTTCATCT GCTCGCTCTC GGGGGACAAC GGCATCATGG GCTGCCACGA AATCCCCCCG 
CTCAAGGAGC AGGGCCGGGA GTGCTGCCTG AGCAAGGACG ACTTCGCCTT CTACAGCGCG 
GCCCAGCAGG ACTTCAACAT CAGCGGCATG TGTGTCAACT GGAACCGCTA TTACAACGTG 
TGCCGCACGG GCAGCGTCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATATTCCA GGTGATCACC CTAGAAGGCT GGGTTGAGAT CATGTACTAT 
GTCATGGACG CCCATTCCTT CTACAACTTC ATCTACTTCA TCCTGCTGAT TATAGTAAGT 
GTGGGGGGCC CAGGAGCCAG GCCGGGAGGG GCCCTTTGGA GAGAAGGGGG TTTCCCGGAT 
CCTTGGGCCT CCTTCTACCT TTCCTTTGGG GCCCTTTGGG AACTACCACA AGAAATTCAT 
AAAACTGACT TTAACCTTTT CCCTTGCCTT CCAGGGCTAT GTCAGAAACA CATTCCCTTG 
GATTACCCAT CCCATACTCT CGTGCAACCC ATCTCTGTGA CACAGACCTC CGACCCTGGC 
AACTGCCCCC ATTGCCAGCA GGAGAGAGAT CAGCGTCTAT CCCTGGCAGA GAGTGATGAT 
GACTTTGGGC GGGAGGAAGG TTCTGCCAAT GATGATGAAG GCGAAGGTGG AGGAAGCAGT 
GAAGGAACTC TGGACCTAGA GAAGGAGGAA GAGGAGGGAG AAAAAGGCAG TGGCGCAGCC 
AGCCTGTGTG GGGATATATG GCGGGAGGTG AGGGCCAAGC TGAGGGGCAT CGTGGACAGC 
AAATATTTTA ACCGAGGCAT CATGATTGCC ATCTTGGTGA ACACTGTCAG CATGGGCATT 
GAACACCACG AACAGCCCGA GGAGCTGACC AACATCCTGG AGATCAGTAA CGTGGTGTTC 
ACCAGCATGT TTGCCCTGGA GATGATCCTG AAGCTGGCGG CCTTCGGCCT CTTCGACTAC 
CTACGGAACC CTTACAACAT CTTTGACAGT ATCATCGTCA TCATCAGTAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTCTCGGTA CTGCGGACGT TCCGCCTGCT TCGAGTCCTG 
AAGCTGGTCC GCTTCATGCC AGCCCTGCGC CGCCAGCTGG TGGTTTTGAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTA 
GGGATGCACA TTTTTGGCTG CAAATTCAGC CTCAAGACTG ACACGGGAGA CACTGTACCC 
GACCGGAAGA ACTTTGATTC GCTTCTTTGG GCCATTGTCA CAGTGTTCCA GATCCTGACC 
CAAGAGGACT GGAATATGGT GCTCTACAAT GGCATGGCCT CCACCTCTCC TTGGGCCTCC 
CTCTACTTTG TGGCTCTCAT GACCTTCGGC AACTACGTGC TCTTCAACCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGG GATGCCAACC GCTCCTACTC AGATGAGGAC 
CAGAGCTCGT CCAACATGGA GGAGTTTGAC AAGTTCCGAG AGGGGCATGA AGGCCATTTT 
GATATCAAGC TCTGCCCCAT CCCCATGACT CCCAACGGGC ACCTGGACCC CAACCTTCCA 
CTGCCCGGGA CCCTGGCCAC CACGGGGGGC ATGCCCTCTG GCCCTTGCCA CTCTCTGCAG 
CCGGACCAGG TGCTCATCGC CCTGGACTCC CGAAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTATG ACCAGCGGTC TCTGTCCAGC TCCCGCAGCT CCTATTATGG GGCCTGGGGC 
CACAGTGGAG CCTGGGCCAG CCGCCGGTCC AGTTGGAACA GCCTGGCCCG GGGTCACAGC 
CTGAAGCACC GGGCACCTTC TGCGGAACAC GAGTCACTCC TCTCTGGGGA GAGGGGCCAG 
TCAGCAGAGG GGGAGCGGGA CGACAGTGCC CCTCGTGGCC CCTCCCATCA CCTACCACCC 
CATACCCATC CCCATCCCCA TCATCACCAC CTCCACCACC CACACCGCTA CCGGCACCGG 
CGGACACTCT CCCTTGATAC CAAGGACTCT TTGGATCTGG CAGAGTTGCC CCTGCCAGCC 
CCTCATGCTC ATCTGAGGTC CACCCGTAAA GTGGGCTCAG GCTTGGGTGC CAGTGAGCAC 
CAGGACTGCA ATGGCAAGAT GCCCAACATT GCCAAGGACG TTTTCACCAA GATGGACAAC 
CGGAAGGACC ACGCGGAGGA TGAGGAAGAG ATTGACTATA GCCTATGTTT CCGGATCCGG 
AAGATGATAG ATGTCTACAA GCCTGACTGG TGTGAAATCA GGGAGGACTG GTCTGTCTAC 
CTCTTCTCTC CTGAGAACAG GTTCCGGGTC CTTTGCCAAA CCATCATCAC TCACAAGCTA 
TTTGACTATG TCGTCCTGGC TTTTATCTTT CTCAACTGTA TCACCATCGC CTTGGAGAGG 
CCCCAGATAG AGGCTGGCAG CACCGAGCGT ATATTCCTCA CTGTGTCCAA CTATATCTTC 
ACCGCCATCT TTGTTGGAGA GATGACCCTA AAGGTGGTCT CCCTGGGCCT GTATTTTGGG 
GAGCAGGCCT ACCTCCGGAG CAGCTGGAAC ATCTTGGATG GCTTCCTGGT CTTCGTGTCC 
CTCATTGACA TTGTGGTCTC TGTGGCTTCT GCGGGAGGAG CTACCATACT TGGGGTCCTC 
CGTGTCCTCC GCCTCCTCCG TACTCTTCGC CCGCTACGGG TCATCAGCCG CGCCCCGGGG 
CTGAAGCTTG TGGTGGAGAC ACTGATCTCA TCCCTCAAAC CCATTGGAAA CATCGTCCTC 
ATCTGCTGCG CCTTCTTCAT CATCTTCGGC ATCCTGGGAG TGCAGCTCTT CAAGGGGAAG 
TTTTACCACT GCCTGGGGGT CGACATCAGG AACATCACCA ACCGCTCCGA CTGCATGGCC 
GCCAACTACC GCTGGGTGCA CCACAAGTAC AACTTTGACA ACCTGGGCCA GGCCTTGATG 
TCTCTCTTTG TCCTGGCCTC CAAAGATGGT TGGGTCAACA TCATGTACAA CGGGCTAGAT 
GCTGTGGCCG TCGACCAGCA GCCCGTGACC AATCACAATC CCTGGATGTT GTTGTACTTC 
ATCTCCTTCC TGCTCATCGT CAGCTTCTTT GTCCTTAACA TGTTTGTGGG TGTGGTGGTG 
GAGAACTTCC ACAAGTGTCG GCAGCACCAG GAGGCAGAGG AGGCGCGTCG GAGGGAGGAG 
AAGCGACTCC GGCGGCTGGA GAAGAAGCGA AGGAAAGCCC AGCGGCTCCC ATATTATGCC 
ACCTACTGCC CTACCCGGCT CCTTATTCAT TCTGTCTGTA CCAGTCACTA CCTGGATATC 
TTCATCACCT TCATCATCTG CCTCAATGTC GTGACCATGT CCTTGGAACA CTACAACCAG 
CCCATGTCCT TGGAGATTGC CCTCAAATAT TGCAACTACA TGTTCACCAC AGTCTTTGTG 
CTGGAAGCCA TGCTGAAGCT GGTAGCATTT GGACTGAGAC GCTTCTTCAA AGACAGGTGG 
AACCAGCTGG ACCTGGCCAT TGTGCTCCTC TCCGTCATGG GTATCACCCT GGAGGAGATT 
GAGATCAACG CCGCCCTGCC CATCAACCCC ACCATCATCC GCATCATGAG GGTGCTTCGG 
ATTGCCCGAG TGCTGAAGCT GCTGAAGATG GCCACCGGGA TGCGAGCCCT GCTGGACACA 
GTGGTTCAGG CCCTCCCCCA GGTGGGAAAC CTGGGTCTTC TCTTCATGCT TCTCTTCTTC 
ATCTATGCTG CTCTGGGCGT GGAGCTCTTT GGGAAGCTGG TGTGCAGCGA TGAGCATCCC 
TGTGAGGGCA TGAGCCGTCA CGCCACCTTT GAAAACTTTG GTATGGCCTT CCTCACGCTA 
TTCCAGGTCT CCACTGGGGA CAACTGGAAT GGGATTATGA AGGACACGCT GCGGGACTGC 
ACCCACGACG AGCGGAGCTG CCTCAGCAAC CTCCAGTTCA TCTCGCCCCT GTACTTCGTG 
AGCTTCGTGC TCACCGCCCA GTTCGTGCTC ATCAACGTGG TGGTGGCCGT GCTGATGAAG 
CACCTGGACG ACAGCAACAA GGAGGCTCAG GAGGACGCCG AGATGGACGC CGAGATCGAG 
CTGGAGATGG CCCACGGCCT CTGCTCGCGG AGCTCCCCGG GCGCCGGCAA GGGCGGGGGC 
TGTGCGGGCC CCGGAGGCCC GGGAGGAGGG CCGCCCCCCG ATCCCGAAGC GCGCCGCTGC 
GTGCGCTGCT ACTCCCCGGC ACAGGAGAAC TTGTGGCTGG ACAGTGTCTC TTTAATTATT 
AAGGACTCCT TCGAAGGAGA GTTAATGATC ATCGATAACC TGTCAGGCTC CGTCTTCCAC 
CATTATTCCT CCCCGGCCGT GTGCGAGAGG TGTGATCATG ACCAGCAAGA GGTCCAGCTG 
GCTGAGGTGG AGGCCTTCTC CCTGAACTCG GACAAGTCCT CTTCAATCCT GCTGGGGGAT 
GATGTCAGCT TGGAGGATCC CACAGCATGC CCTGTGGCCT CCAAGGAGAG CAAGCAAGGA 
GAGTCGGAGT CTCCTGACCC AATCCAGATC GGGGACCTGG GAGAATGCTT CTTCCCGCTG 
TCCAATACGG CGGTCTCGGC AACCCCAGAG AGCTACCTGT GTGAGATGGA GAAGGACCCC 
TTCCATCCCG TCCAGTCCTG GCTGAAACAC GAGAGCAGTC AAACTCCCCC CAGGCCTTTC 
TCACCCGATG CCGCCAACCC CCTCCTGCCC GTGCCAGCAG AGTTCTTCCA CCCAGCTGTG 
GCCACCAGCC AGAAGGGGGA GGAGAAGCCT TCCTGCAAGG GGACCCTCCC CAAGATCTCC 
CTGCAGGGCT CCTGGGCATC CTTACGCTCT CCAAGTGTCA ACTGTGCCCT CCTCAGACAG 
ACCACAGAGA GCGACACCTC CCTGGAGGAC AGCAGGAGCA GCGGCTCGGT GGGCAGCCTG 
CAGACCACGC TGGACGACAG CCTGAATCTC AGCGACTCCC CGCAGTGTAC CCTGGACCTC 
CCGGCCGCCC TGTGCCCGGG GCTGCCGGGC CCCCACGCAC GGGCCGCCCA CAGGACTGTC 
GTGGCTGCGG CGCTCTCTCC GGCCTCCCGT CGCCGGAGCC TCCGAGGCCG CGGCCTCTTC 
AGCCTGCGGA GCCTCCGGGG CCACCAGCGC AGCCACAGCA GCGGGGGCAG CACCAGTCCC 
GGCTGCACCT ACCATGACTC TATGGACCCC TCGGACGAGG AGGGCATGGG CAGCAGCCTC 
CGGGGCGGGG GCACCAACAG CAGTGAGCAG TCGGAGACCC TCAGCAGCCT CTCGCTCACG 
TCCCTCTTCT CCCCGGCCCT CCCGCCCCCG GGCCTCCGGC TAGCTAAGAG GTGCAACAGC 
ACAAGCAGCC TGTCGACGGT GGCCCCCTTG TCCCCCGCCC AGGGCCCCCT GCCGCCCGTG 
GGGACCGAGG GCATGCAGTC CTTCTCGGTT GACCCCAGGG GCTTCCTGTC TGCGCCCTCC 
TGGGGGACAG ACTTCTGCAA GGACCGCCAC TCAGCCAGCG AGGAGACCCA GGGGCTGGGC 
GGCCGTGAGC GCAAGCAGCG CCCCGAGGTG GAGCTCGGGG AAGGGGTGAC CAAGCGGAAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795983.1
CDS	52..6297
Translation	MLVILLNCVTLGMYQPCEDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGRKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEDNFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDFAFYSAAQQDFNISGMCVNWNRYYNVCRTGSVNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVSVGGPGARPGGALWREGGFPDPWASFYLSFGALWELPQEIHKTDFNLFPCLPGLCQKHIPLDYPSHTLVQPISVTQTSDPGNCPHCQQERDQRLSLAESDDDFGREEGSANDDEGEGGGSSEGTLDLEKEEEEGEKGSGAASLCGDIWREVRAKLRGIVDSKYFNRGIMIAILVNTVSMGIEHHEQPEELTNILEISNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFDKFREGHEGHFDIKLCPIPMTPNGHLDPNLPLPGTLATTGGMPSGPCHSLQPDQVLIALDSRKSSVMSLGRMSYDQRSLSSSRSSYYGAWGHSGAWASRRSSWNSLARGHSLKHRAPSAEHESLLSGERGQSAEGERDDSAPRGPSHHLPPHTHPHPHHHHLHHPHRYRHRRTLSLDTKDSLDLAELPLPAPHAHLRSTRKVGSGLGASEHQDCNGKMPNIAKDVFTKMDNRKDHAEDEEEIDYSLCFRIRKMIDVYKPDWCEIREDWSVYLFSPENRFRVLCQTIITHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNILDGFLVFVSLIDIVVSVASAGGATILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDIRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSVCTSHYLDIFITFIICLNVVTMSLEHYNQPMSLEIALKYCNYMFTTVFVLEAMLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCSDEHPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLCSRSSPGAGKGGGCAGPGGPGGGPPPDPEARRCVRCYSPAQENLWLDSVSLIIKDSFEGELMIIDNLSGSVFHHYSSPAVCERCDHDQQEVQLAEVEAFSLNSDKSSSILLGDDVSLEDPTACPVASKESKQGESESPDPIQIGDLGECFFPLSNTAVSATPESYLCEMEKDPFHPVQSWLKHESSQTPPRPFSPDAANPLLPVPAEFFHPAVATSQKGEEKPSCKGTLPKISLQGSWASLRSPSVNCALLRQTTESDTSLEDSRSSGSVGSLQTTLDDSLNLSDSPQCTLDLPAALCPGLPGPHARAAHRTVVAAALSPASRRRSLRGRGLFSLRSLRGHQRSHSSGGSTSPGCTYHDSMDPSDEEGMGSSLRGGGTNSSEQSETLSSLSLTSLFSPALPPPGLRLAKRCNSTSSLSTVAPLSPAQGPLPPVGTEGMQSFSVDPRGFLSAPSWGTDFCKDRHSASEETQGLGGRERKQRPEVELGEGVTKRNR

Target ORF information:

RefSeq Version	XM_031940123.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031940123.1

ORF Insert Sequence:

ATGTTGGTGA TTCTCCTCAA CTGTGTCACC CTGGGCATGT ACCAGCCATG TGAGGACATG 
GATTGTCTGT CTGACCGCTG CAAGATCCTG CAGGTTTTTG ATGACTTCAT CTTCATCTTC 
TTTGCCATGG AGATGGTGCT GAAGATGGTG GCCCTGGGGA TTTTCGGCAG GAAGTGTTAC 
CTGGGGGACA CCTGGAACCG ACTGGATTTC TTCATTGTGA TGGCCGGGAT GGTGGAATAT 
TCTCTGGACC TTCAGAACAT CAACCTGTCA GCCATCCGCA CAGTCCGAGT CCTGAGGCCC 
CTCAAAGCCA TCAATCGCGT GCCCAGTATG CGCATCCTCG TGAACCTGCT GCTGGACACC 
CTGCCCATGT TGGGCAACGT ACTGCTGTTG TGTTTCTTTG TCTTCTTCAT CTTTGGGATA 
ATCGGTGTGC AGCTGTGGGC CGGCCTGCTT CGGAACCGCT GCTTTCTGGA GGACAACTTC 
ACCATACAAG GGGATGTGGC CCTCCCTCCC TACTACCAGC CGGAGGAGGA TGACGAGATG 
CCGTTCATCT GCTCGCTCTC GGGGGACAAC GGCATCATGG GCTGCCACGA AATCCCCCCG 
CTCAAGGAGC AGGGCCGGGA GTGCTGCCTG AGCAAGGACG ACTTCGCCTT CTACAGCGCG 
GCCCAGCAGG ACTTCAACAT CAGCGGCATG TGTGTCAACT GGAACCGCTA TTACAACGTG 
TGCCGCACGG GCAGCGTCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATATTCCA GGTGATCACC CTAGAAGGCT GGGTTGAGAT CATGTACTAT 
GTCATGGACG CCCATTCCTT CTACAACTTC ATCTACTTCA TCCTGCTGAT TATAGTAAGT 
GTGGGGGGCC CAGGAGCCAG GCCGGGAGGG GCCCTTTGGA GAGAAGGGGG TTTCCCGGAT 
CCTTGGGCCT CCTTCTACCT TTCCTTTGGG GCCCTTTGGG AACTACCACA AGAAATTCAT 
AAAACTGACT TTAACCTTTT CCCTTGCCTT CCAGGGCTAT GTCAGAAACA CATTCCCTTG 
GATTACCCAT CCCATACTCT CGTGCAACCC ATCTCTGTGA CACAGACCTC CGACCCTGGC 
AACTGCCCCC ATTGCCAGCA GGAGAGAGAT CAGCGTCTAT CCCTGGCAGA GAGTGATGAT 
GACTTTGGGC GGGAGGAAGG TTCTGCCAAT GATGATGAAG GCGAAGGTGG AGGAAGCAGT 
GAAGGAACTC TGGACCTAGA GAAGGAGGAA GAGGAGGGAG AAAAAGGCAG TGGCGCAGCC 
AGCCTGTGTG GGGATATATG GCGGGAGGTG AGGGCCAAGC TGAGGGGCAT CGTGGACAGC 
AAATATTTTA ACCGAGGCAT CATGATTGCC ATCTTGGTGA ACACTGTCAG CATGGGCATT 
GAACACCACG AACAGCCCGA GGAGCTGACC AACATCCTGG AGATCAGTAA CGTGGTGTTC 
ACCAGCATGT TTGCCCTGGA GATGATCCTG AAGCTGGCGG CCTTCGGCCT CTTCGACTAC 
CTACGGAACC CTTACAACAT CTTTGACAGT ATCATCGTCA TCATCAGTAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTCTCGGTA CTGCGGACGT TCCGCCTGCT TCGAGTCCTG 
AAGCTGGTCC GCTTCATGCC AGCCCTGCGC CGCCAGCTGG TGGTTTTGAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTA 
GGGATGCACA TTTTTGGCTG CAAATTCAGC CTCAAGACTG ACACGGGAGA CACTGTACCC 
GACCGGAAGA ACTTTGATTC GCTTCTTTGG GCCATTGTCA CAGTGTTCCA GATCCTGACC 
CAAGAGGACT GGAATATGGT GCTCTACAAT GGCATGGCCT CCACCTCTCC TTGGGCCTCC 
CTCTACTTTG TGGCTCTCAT GACCTTCGGC AACTACGTGC TCTTCAACCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGG GATGCCAACC GCTCCTACTC AGATGAGGAC 
CAGAGCTCGT CCAACATGGA GGAGTTTGAC AAGTTCCGAG AGGGGCATGA AGGCCATTTT 
GATATCAAGC TCTGCCCCAT CCCCATGACT CCCAACGGGC ACCTGGACCC CAACCTTCCA 
CTGCCCGGGA CCCTGGCCAC CACGGGGGGC ATGCCCTCTG GCCCTTGCCA CTCTCTGCAG 
CCGGACCAGG TGCTCATCGC CCTGGACTCC CGAAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTATG ACCAGCGGTC TCTGTCCAGC TCCCGCAGCT CCTATTATGG GGCCTGGGGC 
CACAGTGGAG CCTGGGCCAG CCGCCGGTCC AGTTGGAACA GCCTGGCCCG GGGTCACAGC 
CTGAAGCACC GGGCACCTTC TGCGGAACAC GAGTCACTCC TCTCTGGGGA GAGGGGCCAG 
TCAGCAGAGG GGGAGCGGGA CGACAGTGCC CCTCGTGGCC CCTCCCATCA CCTACCACCC 
CATACCCATC CCCATCCCCA TCATCACCAC CTCCACCACC CACACCGCTA CCGGCACCGG 
CGGACACTCT CCCTTGATAC CAAGGACTCT TTGGATCTGG CAGAGTTGCC CCTGCCAGCC 
CCTCATGCTC ATCTGAGGTC CACCCGTAAA GTGGGCTCAG GCTTGGGTGC CAGTGAGCAC 
CAGGACTGCA ATGGCAAGAT GCCCAACATT GCCAAGGACG TTTTCACCAA GATGGACAAC 
CGGAAGGACC ACGCGGAGGA TGAGGAAGAG ATTGACTATA GCCTATGTTT CCGGATCCGG 
AAGATGATAG ATGTCTACAA GCCTGACTGG TGTGAAATCA GGGAGGACTG GTCTGTCTAC 
CTCTTCTCTC CTGAGAACAG GTTCCGGGTC CTTTGCCAAA CCATCATCAC TCACAAGCTA 
TTTGACTATG TCGTCCTGGC TTTTATCTTT CTCAACTGTA TCACCATCGC CTTGGAGAGG 
CCCCAGATAG AGGCTGGCAG CACCGAGCGT ATATTCCTCA CTGTGTCCAA CTATATCTTC 
ACCGCCATCT TTGTTGGAGA GATGACCCTA AAGGTGGTCT CCCTGGGCCT GTATTTTGGG 
GAGCAGGCCT ACCTCCGGAG CAGCTGGAAC ATCTTGGATG GCTTCCTGGT CTTCGTGTCC 
CTCATTGACA TTGTGGTCTC TGTGGCTTCT GCGGGAGGAG CTACCATACT TGGGGTCCTC 
CGTGTCCTCC GCCTCCTCCG TACTCTTCGC CCGCTACGGG TCATCAGCCG CGCCCCGGGG 
CTGAAGCTTG TGGTGGAGAC ACTGATCTCA TCCCTCAAAC CCATTGGAAA CATCGTCCTC 
ATCTGCTGCG CCTTCTTCAT CATCTTCGGC ATCCTGGGAG TGCAGCTCTT CAAGGGGAAG 
TTTTACCACT GCCTGGGGGT CGACATCAGG AACATCACCA ACCGCTCCGA CTGCATGGCC 
GCCAACTACC GCTGGGTGCA CCACAAGTAC AACTTTGACA ACCTGGGCCA GGCCTTGATG 
TCTCTCTTTG TCCTGGCCTC CAAAGATGGT TGGGTCAACA TCATGTACAA CGGGCTAGAT 
GCTGTGGCCG TCGACCAGCA GCCCGTGACC AATCACAATC CCTGGATGTT GTTGTACTTC 
ATCTCCTTCC TGCTCATCGT CAGCTTCTTT GTCCTTAACA TGTTTGTGGG TGTGGTGGTG 
GAGAACTTCC ACAAGTGTCG GCAGCACCAG GAGGCAGAGG AGGCGCGTCG GAGGGAGGAG 
AAGCGACTCC GGCGGCTGGA GAAGAAGCGA AGGAAAGCCC AGCGGCTCCC ATATTATGCC 
ACCTACTGCC CTACCCGGCT CCTTATTCAT TCTGTCTGTA CCAGTCACTA CCTGGATATC 
TTCATCACCT TCATCATCTG CCTCAATGTC GTGACCATGT CCTTGGAACA CTACAACCAG 
CCCATGTCCT TGGAGATTGC CCTCAAATAT TGCAACTACA TGTTCACCAC AGTCTTTGTG 
CTGGAAGCCA TGCTGAAGCT GGTAGCATTT GGACTGAGAC GCTTCTTCAA AGACAGGTGG 
AACCAGCTGG ACCTGGCCAT TGTGCTCCTC TCCGTCATGG GTATCACCCT GGAGGAGATT 
GAGATCAACG CCGCCCTGCC CATCAACCCC ACCATCATCC GCATCATGAG GGTGCTTCGG 
ATTGCCCGAG TGCTGAAGCT GCTGAAGATG GCCACCGGGA TGCGAGCCCT GCTGGACACA 
GTGGTTCAGG CCCTCCCCCA GGTGGGAAAC CTGGGTCTTC TCTTCATGCT TCTCTTCTTC 
ATCTATGCTG CTCTGGGCGT GGAGCTCTTT GGGAAGCTGG TGTGCAGCGA TGAGCATCCC 
TGTGAGGGCA TGAGCCGTCA CGCCACCTTT GAAAACTTTG GTATGGCCTT CCTCACGCTA 
TTCCAGGTCT CCACTGGGGA CAACTGGAAT GGGATTATGA AGGACACGCT GCGGGACTGC 
ACCCACGACG AGCGGAGCTG CCTCAGCAAC CTCCAGTTCA TCTCGCCCCT GTACTTCGTG 
AGCTTCGTGC TCACCGCCCA GTTCGTGCTC ATCAACGTGG TGGTGGCCGT GCTGATGAAG 
CACCTGGACG ACAGCAACAA GGAGGCTCAG GAGGACGCCG AGATGGACGC CGAGATCGAG 
CTGGAGATGG CCCACGGCCT CTGCTCGCGG AGCTCCCCGG GCGCCGGCAA GGGCGGGGGC 
TGTGCGGGCC CCGGAGGCCC GGGAGGAGGG CCGCCCCCCG ATCCCGAAGC GCGCCGCTGC 
GTGCGCTGCT ACTCCCCGGC ACAGGAGAAC TTGTGGCTGG ACAGTGTCTC TTTAATTATT 
AAGGACTCCT TCGAAGGAGA GTTAATGATC ATCGATAACC TGTCAGGCTC CGTCTTCCAC 
CATTATTCCT CCCCGGCCGT GTGCGAGAGG TGTGATCATG ACCAGCAAGA GGTCCAGCTG 
GCTGAGGTGG AGGCCTTCTC CCTGAACTCG GACAAGTCCT CTTCAATCCT GCTGGGGGAT 
GATGTCAGCT TGGAGGATCC CACAGCATGC CCTGTGGCCT CCAAGGAGAG CAAGCAAGGA 
GAGTCGGAGT CTCCTGACCC AATCCAGATC GGGGACCTGG GAGAATGCTT CTTCCCGCTG 
TCCAATACGG CGGTCTCGGC AACCCCAGAG AGCTACCTGT GTGAGATGGA GAAGGACCCC 
TTCCATCCCG TCCAGTCCTG GCTGAAACAC GAGAGCAGTC AAACTCCCCC CAGGCCTTTC 
TCACCCGATG CCGCCAACCC CCTCCTGCCC GTGCCAGCAG AGTTCTTCCA CCCAGCTGTG 
GCCACCAGCC AGAAGGGGGA GGAGAAGCCT TCCTGCAAGG GGACCCTCCC CAAGATCTCC 
CTGCAGGGCT CCTGGGCATC CTTACGCTCT CCAAGTGTCA ACTGTGCCCT CCTCAGACAG 
ACCACAGAGA GCGACACCTC CCTGGAGGAC AGCAGGAGCA GCGGCTCGGT GGGCAGCCTG 
CAGACCACGC TGGACGACAG CCTGAATCTC AGCGACTCCC CGCAGTGTAC CCTGGACCTC 
CCGGCCGCCC TGTGCCCGGG GCTGCCGGGC CCCCACGCAC GGGCCGCCCA CAGGACTGTC 
GTGGCTGCGG CGCTCTCTCC GGCCTCCCGT CGCCGGAGCC TCCGAGGCCG CGGCCTCTTC 
AGCCTGCGGA GCCTCCGGGG CCACCAGCGC AGCCACAGCA GCGGGGGCAG CACCAGTCCC 
GGCTGCACCT ACCATGACTC TATGGACCCC TCGGACGAGG AGGGCATGGG CAGCAGCCTC 
CGGGGCGGGG GCACCAACAG CAGTGAGCAG TCGGAGACCC TCAGCAGCCT CTCGCTCACG 
TCCCTCTTCT CCCCGGCCCT CCCGCCCCCG GGCCTCCGGC TAGCTAAGAG GTGCAACAGC 
ACAAGCAGCC TGTCGACGGT GGCCCCCTTG TCCCCCGCCC AGGGCCCCCT GCCGCCCGTG 
GGGACCGAGG GCATGCAGTC CTTCTCGGTT GACCCCAGGG GCTTCCTGTC TGCGCCCTCC 
TGGGGGACAG ACTTCTGCAA GGACCGCCAC TCAGCCAGCG AGGAGACCCA GGGGCTGGGC 
GGCCGTGAGC GCAAGCAGCG CCCCGAGGTG GAGCTCGGGG AAGGGGTGAC CAAGCGGAAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CACNA1I cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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