HEATR1 cDNA ORF clone, Saccoglossus kowalevskii

Gene
Clones
gRNAs

Summary

Gene Symbol	HEATR1
Entrez Gene ID	100373273
Full Name	HEAT repeat containing 1
Gene Type	protein-coding
Organism	Saccoglossus kowalevskii

mRNA and Protein(s)

mRNA	Protein	Name
XM_002737405.2	XP_002737451.2	LOW QUALITY PROTEIN: HEAT repeat-containing protein 1

Pathways from BioSystems
Gene Ontology

The following HEATR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEATR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSg08836	XM_002737405.2 Latest version!	Saccoglossus kowalevskii HEAT repeat containing 1 (HEATR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEATR1 ( XM_002737405.2 ) cDNA ORF clone, Saccoglossus kowalevskii -> XP_002737451.2 Saccoglossus kowalevskii HEAT repeat containing 1 (HEATR1), mRNA.

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CloneID	OSg08836
Clone ID Related Accession (Same CDS sequence)	XM_002737405.2
Accession Version	XM_002737405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1392566400000
Organism	Saccoglossus kowalevskii
Product	LOW QUALITY PROTEIN: HEAT repeat-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003135368.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 18, 2014 this sequence version replaced XM_002737405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Saccoglossus kowalevskii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCAT TGGCTTCTCA GCTGAAGAAA CTTGCCTTAC CCCAGACGCA GCAGAGTCAG 
TTGTCTAGAG ACAGAGAAAA GGCATCTTTG TTGTTTGATC CTAAAGAAGC ATCAAGTTTA 
GACAGAGAAA CTTTTTATGC ATTAGGGGTA AATGGTCTAG AAGAATTGAT AGGAATTGAT 
CCTTCATTCC AAGATTACGA AGTCACGTTA TTCGACGAAG CATCAAAATC TCTGGAGAGA 
TCCATCCAAT CGGTTGAAAT AAACCAAAAA CTGAACAAAA AGATATCTGA ATTCTTGTTG 
AGATTGTCAC CGTATTTTCT GTTGAAGCCA GCACAGAAGA CATTGGAGTG GTTGATACAT 
AGATTTTATA TACACCAGTA TAATGTCGAT GATTTGTTAA TGTGCGTCCT GCCGTATCAT 
GAGACTATGA TATTTGTACG AGTTATACAG CTATTAAAAG TAGCGGATCC CACACATAAA 
TGGCATTGGC TTCATCCAAT ACAGAAACCC GGGGTAGCCC TTGCAAGATC AACTTTACTA 
AATCACTGCT ACAAAGATGC TGGATTTCTA CATTTTATTT GTGAAATGGT TCCCAAGTCT 
ATCAAGGCAC ATGGTGTAAC GTCGTCAAGT ACAGCATCTC TACGAGTTCT ATTTTCATTT 
TACACCTCCA CCGTAATTGG TATGATAGAA TACTGCAATA CAGTTACCGA GATATTGCTG 
TCGAACCTGA TACCGTACAT CCTAAAAGGA TTGAAATCGA CGTACTCTGA CTACAAAGCG 
GCATCGTACA TGATAATTTG TCAATTGGTG ACAAAAACTA CACTGAAAAT GAACTTTCTT 
GATCCATTTA TCAAGTCTGT ATGCAAGCAT ACGAGTGGTG ATCTAGCTGC CGAGGCTGTC 
ATGTGTATTG TCGTAATCTG CCAAACACAG AAGATTGAAA AACTGCCAAA AAAGGCTGTT 
AAAAGTTTAT CAAAATTGGA TGGCATAATA CAGATATTAG AGCAGTTGTC GTTATCAACA 
AATATAAGAC CATTCATGGA AGTATTTCTA CAAAGAATTG TAACAAATAC TTTTGCTGAA 
GGCGTTGCTA TGGCAACAAG TAGTGAGGGT GAAAATGATT TTTCAGCTGG TCCTGTTTAT 
AGTACTTTAC TTGAGAATGT CATCCATGAT GTTGCCATGA CAACAGAGAC CGTTGATATA 
TTAGCAAGGT TATTGATTCA GGAGTTCTTG GAGTTCTCAC TTGATGAAGA CAAACGATAT 
TCAACAGAAA ATGTACAGGT TATAAAGGAA AAAATCCATC CGATTATACA AGCATTGGAA 
CAAAAGTACC CAGTTTCAGT TGATAAAGCT TTGGAAAATA TGTTAAAAGA AATTGAGAGC 
CAAAAAGAAA AAGACATACT GCAGGAGTTC ATATCCATGA CAGTTTCAAG ATCCAAATAT 
CAGGTTGTTG ATGAATCACA GACTTCACTC TTATTGAGTT TAAATCATCC TAGTGGCAGG 
ATTAGACACT CTGCTGTCAA GTATCTGCTG ACTAAGATAC AGACTGGTGA ATTGGATGAA 
TTCTATCAAG AGTCTTTGCT TCAGAGGCTT TCCGATGATG ACCCCACTGT AGTGAAAGCA 
GTATTAGATT TAGATGAGAA ATTGGTTGAT GTGTTGCCAG GCGACAAGCT ATATGTGGCT 
CTATGTGGAT TATTGAGTGT TTGGAGTACT CATTCAAATG AATCTTGGTG GAGTATTGAA 
CATTGTGTCC TAAGTATTCT CTGTAGTCAG AATTTTTTTA ACAAGATGGC CGCCAAGATA 
GATGACTGTG CATTGGCTGT ACTGCCATAT CTTTATCTAG TGAGAAGTAA AGAACCACTG 
AAAGAGAAAC AAATAGCTCT AACTATAGCA ACATCTGCAT TGTCTAAAAA ACACAAAGTA 
TTGGCTAACC TCAGTGAAAG TTACGTTGCA CTCACTGCCA AATCCAACAA GGATACAGTA 
GCTGAAGTGA ATGAACGCAC GGTTACAAAG ATTACTGAAA ATATGTTGAA AATGAAAGAA 
AGTGATGTCC TGAAAATGAT ACACTTGCTG TCAGATCAAG TGACAGACCA CAGTACAAAT 
CTAAGAGTTA AAATGGTGGC TAAATTCATT CTGGCAAGTT TAGTTGCCAT GTCAACAAAC 
ATTCGATTGC AGCTTTCTAT TGCTACCACC CTGTTGTCAG CTTTAGGAGA TGATGTCAAT 
AGAATAATCG ATGTTGTGCC AAAGAATATG GATGAAGATG ATCTGGATAC CATGGAAACT 
GAAATGCTGA CTGCTGATAC ATCACACATC CACTGTAAAG CCTTAAAAGT CTTGTCTAAA 
AAGAAAAACC GAATGGTTAA GTGTCCCGAG TTTGCGGATA CTGTGTTTTG GTTGTTGGAT 
AGCATAGTGA TGAATATTCC CTTGCAGACA TTGTTGAAAG GATCACATTG GTTGAATTGT 
GATAAAAAAC CAGACAGCCA GGATACAGAA TATATCAAAT TGTTGTTAAA ACTTTTTGAA 
TATTTTACTG CTGGGTCAGC TGACAGCAAA TCAGCCAATC ACGTTGCTGC ATTTAGGAAT 
CTATTGAAGA AACTGATTGA GCATCATTTA AAGGAAAGAT CTAAACTTTG GATGTTTTTG 
TCCGTTATGT GGACCAGCCA TTTTGAAGAG TTCCCTCTAG TGCCAGCAGT TGTTCAAATG 
CGTGCACTTC ATATTGCCAA TACCAGTTTC TCTCATCTAG CTGATGAGGA CATGCAGGAT 
CTGTTTAGAA AAGACTCCTT AGTGATACCA TCTTTGCTGG TGGTTCTTTC CTGTTCAATT 
CAACCAATCA GAGCCGCTGG TGTTTTGTGT TTGAAGACCA TTGCTAAATC ATGTAGTGAT 
GTCAGTTCTG TGTACCAGTC ACTAATCACT ACCCTAATCT CTCACACTGA AGAGATTACA 
GCTGATCCTA ATCACATTAA ACAGACGTTA GAGATTCTTT TTGCCAATGT TATCATAGTT 
ACAAAGTCAG TAAGCCCAAA GAAGAAATCT ACCAAACAGG ATAGTGACAT TAGGATGTTG 
TTATCTTGCT TGTTGGACAT CGTTGTTAGC ACTGATGTGC CATATCACGT TCAACAAAAT 
CTTTTGGCTG TCTTATCTAA CGTCAATAAT AAGATGATGC TGTTTAAATT ATTACCAATG 
CTGGAGAATA AATTGGAGAA GATGAAACAG AATAATTTGT CCAAAGCGGA GTGCTTAACT 
GTACAATGGA TTATACGTCA GTATACAAAC GTTGTCACCG AGGTCTTAGA AGATGACTCC 
CTGGCATTGG ATCTATTCAT GAAATGCTTA AATCTACATG AGCGCCTTCA CAACACGGTA 
GCATCACCAC AAGAGCTAGT GCTTCAACAG ATAACCAAAG AGTTTTATTC CAGTATCCCA 
TCATCCTCAG TTCAGCAAAG TATACTCTGT CTATTATTTG ACCTCCATGT TGACAGTACA 
AGTTCACAGA CAGCTACACT GATCAGTAAG ACTTTAAAAG GATTACGACT TGACGCTGAA 
CAGATAATCA TTGAACTGAA CAAAGTCAAC AAATGCACTG GAGCAGCCAC TGTCAAACAA 
GCAAAGCGAG TTAAAAGGCA AGAGAAGGCA ACAGATGATG GTGATAATGT CTTTGAGAGC 
AGAGAATGGA TGCGAATTAC TCTGATTTGT GAATTTTTAC AACACAAAAA GAAAATCAAC 
AATTCTCAGC TTCTTGTTCC TGTTCTGTTT AACCTTCTGT CACAGTGCTT TGAAGCTGAG 
ACAGCTGACC AGATTTCTAC GGAATATATT AAACAGTTAA TACTGAGTTC TATACTCAAT 
ATTTGTAATA AACTTTCACC GGACAACAAA CCGTTGCCCT CAGATGTACT GACTGAAGAG 
CAGTTTAAAG TTGAGTTGAT AATTCAGTGC ATACGAGTCT CGGACAATCC ACAAACGCAT 
CACCATGCCA TGTTGCTATT GGCAACTGCT GCTGGTATAT TTCCTGAACA TGTACTTCAT 
AACATCATGT CAATCTTTAC GTTTATGGGA ACTAATCTAC TACGGCAAGA TGATTCTTAC 
AGTTCTCAAG TAATCAATAA AATTGTTGAA ACGGTAATAC CTGTCTTGAT TAAGGCAAGT 
GAGGAAGAAA TCCTAAAAGA AAAACTTGGA GGAGGGATAG ATGAGATTGT TGCCATGGTG 
ATCCAGGTAT TCGTTGATGC ATTGCCACAC ATTCCTGCTC ATCGTAAACA GCCGGTGTTT 
CATCAACTGA TTGGTACGCT TGGAGGAGAC AGATTTTTGT GGATGACTGT CGTACTGTTG 
TTGGTATGCC ATGTAGACAC ATCTACACAG AAGGATGACG TGGTTGATAA GGGTATCACT 
GGTTTGAATA CAGATATTGA ATTCTGTCTG TCAATCTGTA ATAACTGTGC ACCCCATGTG 
CAAGTCAATA ATATGATTAA AATATTGAAA TATCTTGGCA CATTGCCAGA AGACAAAGAA 
GAATCTGCAA CCCCTCACAA GAGAATCATA GGAACAAGAC GGAAATCACA ACTTAAGAAA 
ACTCATCAAT TATTTGATGT TGAGAAACAT ACAGCTAAAC AATTAAGACA GTTCAAATAT 
ACTGTGGTTA ATTTCATCCC CCATTTAATA TCTAGTTCTA CTTACATTAG TCAGGTTGCA 
GCTTTGTCAG TAGAGGAAAC AAACACTATG AAAACACTAT ATCAAAGTCT ATTAGAAGAA 
AACCTACGAT ACATTGCATG TATAGCCAGA TTGCTGGAGA AAAACCATGA CAAACCCACT 
GTGAAATTCT GGAGAGCTTT GTTACATAAA GCCTATGATG TATTGGACAA GATAAATGCA 
TTACTACCAA CACATATATT CATCAATGTG GTATCTGGTC TCATGCATAA TGACATCGCT 
GCCGTACGAC GTAAAGCTAT GGAGTTATTA AATAACAAAC TAACGCAACA TAAAGAACCG 
TTCAATGAAC AACAGGTGTC ACTTTTACTT GCGCTTGTTC CAGAACTTGT ATCAGTAACC 
AAGGCAACCA AAATAGTCGG TGGTGGTTTG TCAGAACTTG ATGTCAACCG ACAGACCGCC 
CTCTATAGTC TCAAGTTATT GTGTAAAGTG TTGGGTTGTG ATAATCCGCA ACACTTTACT 
CCAGTAATGG ATATCGTCAC TAAAGTGTTC TCAACAAAAG ACATCAACTC CCAGGTTGCT 
GCTAGTGCAC TGCTATGCTT GGCTGAGCTG TGTTCCAGTC TGAAAGCCCA TTGTATTCCT 
TATTTGAACA AATTTATGCC TTCATTGGTA ACAATTCTTG ATAATAAGGA CCATCTAAAA 
AGTCATGATT TGCTATTACT GAGTGCAGTT ACTGCGTTAC ATAAAGTTGT GGATAATCTG 
ACTCACTTCC TGAGCCCCTA TCTGTTGGAT ATTTTGACAA AGGTGTCTGC TCTGTTTGCG 
CTGGATAAAG ACGAGAAATC CCAACTAACG ATGAGACTTA AAGCAATTCA TCAGAAGTTA 
TCTTCCACTG TGTCTCCAAG AGTTCTTCTA CCTGTCATAA CACAATGTTA TCACCATGTC 
GTAGACGAAC ATGAGCTTTC AATTATACCT TTGATGTCAG TCCTTGGGGA TAGTATTTCA 
AGTATGTCTA AAGAAGATAT GACTGCTCAT CAGACGTCTC TACTCAATCT ATTCCTGTCT 
GTATTCGATT ACAGAGCATC TCATCCTCAG GTATCTATGG TTACCATTGA AGAAATTGAA 
AGAAGTTCCA TAAATGCTTT CTTGTCATTG GTTATGAAGT CATCTGAAGC AACATTCAAA 
CCAATATTTT TCAAGCTCTA TGATTGGGCT ACACAGACTG GAGCTCATAA AGAGAGGTTA 
TTGACTTTTT ACCGTCTATC TGATAACATA GCTGAAAAGC TAAAGGGATT ATTTACTCTC 
TTTGCTGGAC ATATTGTAAA AAACTGTGCT ACTTTGTTGA ATGACAACAA TTCTTCAAAA 
ACAGATTTAA AGTTTTTTGA GATCGATGGG GATGAAGATG AAGAAGCCCA GAGTGTAGAT 
GAGAAATCTA CTTTGTTGCT ACAATACATC ATTGGTTGTC TTCATAAGTG TTTTCTGTAT 
GATACAGAAG GATTTCTGAA TCGGGAGAGA TTTGAATGTT TGATGCAACC AATAGTAGAT 
CAGATTGATA ACCTACAAGG CGGTATTGAA GTCTACAAAG AAAGAATGAC ATCATATCTT 
ACACCATGTA TTGGTCAGCT GCTTGTTGCT GCTGGTGATG ATTCGTCATG GAAACCGCTT 
AATTATCAAG TTTTGTTGAA GATGAGAAGT GATTCTGCAA AGGTCCGCTT TGCTGCACTT 
ACAGTGTTAC AAGAAATTCA TAGTAAACTT GGTGAAGATT TTCAGATTCT GATTCCAGAA 
GCTATTCCAT TCTTGGCTGA ACTATTAGAA GATGAATCAG AAGAAGTTGA AAAACAGTGC 
CATCAAGTTA TAGAAGAGAT TGAGAAAACA TTTGGGGAGT CGTTACAAAA ATATTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002737451.2
CDS	141..6680
Translation	MTSLASQLKKLALPQTQQSQLSRDREKASLLFDPKEASSLDRETFYALGVNGLEELIGIDPSFQDYEVTLFDEASKSLERSIQSVEINQKLNKKISEFLLRLSPYFLLKPAQKTLEWLIHRFYIHQYNVDDLLMCVLPYHETMIFVRVIQLLKVADPTHKWHWLHPIQKPGVALARSTLLNHCYKDAGFLHFICEMVPKSIKAHGVTSSSTASLRVLFSFYTSTVIGMIEYCNTVTEILLSNLIPYILKGLKSTYSDYKAASYMIICQLVTKTTLKMNFLDPFIKSVCKHTSGDLAAEAVMCIVVICQTQKIEKLPKKAVKSLSKLDGIIQILEQLSLSTNIRPFMEVFLQRIVTNTFAEGVAMATSSEGENDFSAGPVYSTLLENVIHDVAMTTETVDILARLLIQEFLEFSLDEDKRYSTENVQVIKEKIHPIIQALEQKYPVSVDKALENMLKEIESQKEKDILQEFISMTVSRSKYQVVDESQTSLLLSLNHPSGRIRHSAVKYLLTKIQTGELDEFYQESLLQRLSDDDPTVVKAVLDLDEKLVDVLPGDKLYVALCGLLSVWSTHSNESWWSIEHCVLSILCSQNFFNKMAAKIDDCALAVLPYLYLVRSKEPLKEKQIALTIATSALSKKHKVLANLSESYVALTAKSNKDTVAEVNERTVTKITENMLKMKESDVLKMIHLLSDQVTDHSTNLRVKMVAKFILASLVAMSTNIRLQLSIATTLLSALGDDVNRIIDVVPKNMDEDDLDTMETEMLTADTSHIHCKALKVLSKKKNRMVKCPEFADTVFWLLDSIVMNIPLQTLLKGSHWLNCDKKPDSQDTEYIKLLLKLFEYFTAGSADSKSANHVAAFRNLLKKLIEHHLKERSKLWMFLSVMWTSHFEEFPLVPAVVQMRALHIANTSFSHLADEDMQDLFRKDSLVIPSLLVVLSCSIQPIRAAGVLCLKTIAKSCSDVSSVYQSLITTLISHTEEITADPNHIKQTLEILFANVIIVTKSVSPKKKSTKQDSDIRMLLSCLLDIVVSTDVPYHVQQNLLAVLSNVNNKMMLFKLLPMLENKLEKMKQNNLSKAECLTVQWIIRQYTNVVTEVLEDDSLALDLFMKCLNLHERLHNTVASPQELVLQQITKEFYSSIPSSSVQQSILCLLFDLHVDSTSSQTATLISKTLKGLRLDAEQIIIELNKVNKCTGAATVKQAKRVKRQEKATDDGDNVFESREWMRITLICEFLQHKKKINNSQLLVPVLFNLLSQCFEAETADQISTEYIKQLILSSILNICNKLSPDNKPLPSDVLTEEQFKVELIIQCIRVSDNPQTHHHAMLLLATAAGIFPEHVLHNIMSIFTFMGTNLLRQDDSYSSQVINKIVETVIPVLIKASEEEILKEKLGGGIDEIVAMVIQVFVDALPHIPAHRKQPVFHQLIGTLGGDRFLWMTVVLLLVCHVDTSTQKDDVVDKGITGLNTDIEFCLSICNNCAPHVQVNNMIKILKYLGTLPEDKEESATPHKRIIGTRRKSQLKKTHQLFDVEKHTAKQLRQFKYTVVNFIPHLISSSTYISQVAALSVEETNTMKTLYQSLLEENLRYIACIARLLEKNHDKPTVKFWRALLHKAYDVLDKINALLPTHIFINVVSGLMHNDIAAVRRKAMELLNNKLTQHKEPFNEQQVSLLLALVPELVSVTKATKIVGGGLSELDVNRQTALYSLKLLCKVLGCDNPQHFTPVMDIVTKVFSTKDINSQVAASALLCLAELCSSLKAHCIPYLNKFMPSLVTILDNKDHLKSHDLLLLSAVTALHKVVDNLTHFLSPYLLDILTKVSALFALDKDEKSQLTMRLKAIHQKLSSTVSPRVLLPVITQCYHHVVDEHELSIIPLMSVLGDSISSMSKEDMTAHQTSLLNLFLSVFDYRASHPQVSMVTIEEIERSSINAFLSLVMKSSEATFKPIFFKLYDWATQTGAHKERLLTFYRLSDNIAEKLKGLFTLFAGHIVKNCATLLNDNNSSKTDLKFFEIDGDEDEEAQSVDEKSTLLLQYIIGCLHKCFLYDTEGFLNRERFECLMQPIVDQIDNLQGGIEVYKERMTSYLTPCIGQLLVAAGDDSSWKPLNYQVLLKMRSDSAKVRFAALTVLQEIHSKLGEDFQILIPEAIPFLAELLEDESEEVEKQCHQVIEEIEKTFGESLQKYF

Target ORF information:

RefSeq Version	XM_002737405.2
Organism	Saccoglossus kowalevskii
Definition	Saccoglossus kowalevskii HEAT repeat containing 1 (HEATR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002737405.2

ORF Insert Sequence:

ATGACTTCAT TGGCTTCTCA GCTGAAGAAA CTTGCCTTAC CCCAGACGCA GCAGAGTCAG 
TTGTCTAGAG ACAGAGAAAA GGCATCTTTG TTGTTTGATC CTAAAGAAGC ATCAAGTTTA 
GACAGAGAAA CTTTTTATGC ATTAGGGGTA AATGGTCTAG AAGAATTGAT AGGAATTGAT 
CCTTCATTCC AAGATTACGA AGTCACGTTA TTCGACGAAG CATCAAAATC TCTGGAGAGA 
TCCATCCAAT CGGTTGAAAT AAACCAAAAA CTGAACAAAA AGATATCTGA ATTCTTGTTG 
AGATTGTCAC CGTATTTTCT GTTGAAGCCA GCACAGAAGA CATTGGAGTG GTTGATACAT 
AGATTTTATA TACACCAGTA TAATGTCGAT GATTTGTTAA TGTGCGTCCT GCCGTATCAT 
GAGACTATGA TATTTGTACG AGTTATACAG CTATTAAAAG TAGCGGATCC CACACATAAA 
TGGCATTGGC TTCATCCAAT ACAGAAACCC GGGGTAGCCC TTGCAAGATC AACTTTACTA 
AATCACTGCT ACAAAGATGC TGGATTTCTA CATTTTATTT GTGAAATGGT TCCCAAGTCT 
ATCAAGGCAC ATGGTGTAAC GTCGTCAAGT ACAGCATCTC TACGAGTTCT ATTTTCATTT 
TACACCTCCA CCGTAATTGG TATGATAGAA TACTGCAATA CAGTTACCGA GATATTGCTG 
TCGAACCTGA TACCGTACAT CCTAAAAGGA TTGAAATCGA CGTACTCTGA CTACAAAGCG 
GCATCGTACA TGATAATTTG TCAATTGGTG ACAAAAACTA CACTGAAAAT GAACTTTCTT 
GATCCATTTA TCAAGTCTGT ATGCAAGCAT ACGAGTGGTG ATCTAGCTGC CGAGGCTGTC 
ATGTGTATTG TCGTAATCTG CCAAACACAG AAGATTGAAA AACTGCCAAA AAAGGCTGTT 
AAAAGTTTAT CAAAATTGGA TGGCATAATA CAGATATTAG AGCAGTTGTC GTTATCAACA 
AATATAAGAC CATTCATGGA AGTATTTCTA CAAAGAATTG TAACAAATAC TTTTGCTGAA 
GGCGTTGCTA TGGCAACAAG TAGTGAGGGT GAAAATGATT TTTCAGCTGG TCCTGTTTAT 
AGTACTTTAC TTGAGAATGT CATCCATGAT GTTGCCATGA CAACAGAGAC CGTTGATATA 
TTAGCAAGGT TATTGATTCA GGAGTTCTTG GAGTTCTCAC TTGATGAAGA CAAACGATAT 
TCAACAGAAA ATGTACAGGT TATAAAGGAA AAAATCCATC CGATTATACA AGCATTGGAA 
CAAAAGTACC CAGTTTCAGT TGATAAAGCT TTGGAAAATA TGTTAAAAGA AATTGAGAGC 
CAAAAAGAAA AAGACATACT GCAGGAGTTC ATATCCATGA CAGTTTCAAG ATCCAAATAT 
CAGGTTGTTG ATGAATCACA GACTTCACTC TTATTGAGTT TAAATCATCC TAGTGGCAGG 
ATTAGACACT CTGCTGTCAA GTATCTGCTG ACTAAGATAC AGACTGGTGA ATTGGATGAA 
TTCTATCAAG AGTCTTTGCT TCAGAGGCTT TCCGATGATG ACCCCACTGT AGTGAAAGCA 
GTATTAGATT TAGATGAGAA ATTGGTTGAT GTGTTGCCAG GCGACAAGCT ATATGTGGCT 
CTATGTGGAT TATTGAGTGT TTGGAGTACT CATTCAAATG AATCTTGGTG GAGTATTGAA 
CATTGTGTCC TAAGTATTCT CTGTAGTCAG AATTTTTTTA ACAAGATGGC CGCCAAGATA 
GATGACTGTG CATTGGCTGT ACTGCCATAT CTTTATCTAG TGAGAAGTAA AGAACCACTG 
AAAGAGAAAC AAATAGCTCT AACTATAGCA ACATCTGCAT TGTCTAAAAA ACACAAAGTA 
TTGGCTAACC TCAGTGAAAG TTACGTTGCA CTCACTGCCA AATCCAACAA GGATACAGTA 
GCTGAAGTGA ATGAACGCAC GGTTACAAAG ATTACTGAAA ATATGTTGAA AATGAAAGAA 
AGTGATGTCC TGAAAATGAT ACACTTGCTG TCAGATCAAG TGACAGACCA CAGTACAAAT 
CTAAGAGTTA AAATGGTGGC TAAATTCATT CTGGCAAGTT TAGTTGCCAT GTCAACAAAC 
ATTCGATTGC AGCTTTCTAT TGCTACCACC CTGTTGTCAG CTTTAGGAGA TGATGTCAAT 
AGAATAATCG ATGTTGTGCC AAAGAATATG GATGAAGATG ATCTGGATAC CATGGAAACT 
GAAATGCTGA CTGCTGATAC ATCACACATC CACTGTAAAG CCTTAAAAGT CTTGTCTAAA 
AAGAAAAACC GAATGGTTAA GTGTCCCGAG TTTGCGGATA CTGTGTTTTG GTTGTTGGAT 
AGCATAGTGA TGAATATTCC CTTGCAGACA TTGTTGAAAG GATCACATTG GTTGAATTGT 
GATAAAAAAC CAGACAGCCA GGATACAGAA TATATCAAAT TGTTGTTAAA ACTTTTTGAA 
TATTTTACTG CTGGGTCAGC TGACAGCAAA TCAGCCAATC ACGTTGCTGC ATTTAGGAAT 
CTATTGAAGA AACTGATTGA GCATCATTTA AAGGAAAGAT CTAAACTTTG GATGTTTTTG 
TCCGTTATGT GGACCAGCCA TTTTGAAGAG TTCCCTCTAG TGCCAGCAGT TGTTCAAATG 
CGTGCACTTC ATATTGCCAA TACCAGTTTC TCTCATCTAG CTGATGAGGA CATGCAGGAT 
CTGTTTAGAA AAGACTCCTT AGTGATACCA TCTTTGCTGG TGGTTCTTTC CTGTTCAATT 
CAACCAATCA GAGCCGCTGG TGTTTTGTGT TTGAAGACCA TTGCTAAATC ATGTAGTGAT 
GTCAGTTCTG TGTACCAGTC ACTAATCACT ACCCTAATCT CTCACACTGA AGAGATTACA 
GCTGATCCTA ATCACATTAA ACAGACGTTA GAGATTCTTT TTGCCAATGT TATCATAGTT 
ACAAAGTCAG TAAGCCCAAA GAAGAAATCT ACCAAACAGG ATAGTGACAT TAGGATGTTG 
TTATCTTGCT TGTTGGACAT CGTTGTTAGC ACTGATGTGC CATATCACGT TCAACAAAAT 
CTTTTGGCTG TCTTATCTAA CGTCAATAAT AAGATGATGC TGTTTAAATT ATTACCAATG 
CTGGAGAATA AATTGGAGAA GATGAAACAG AATAATTTGT CCAAAGCGGA GTGCTTAACT 
GTACAATGGA TTATACGTCA GTATACAAAC GTTGTCACCG AGGTCTTAGA AGATGACTCC 
CTGGCATTGG ATCTATTCAT GAAATGCTTA AATCTACATG AGCGCCTTCA CAACACGGTA 
GCATCACCAC AAGAGCTAGT GCTTCAACAG ATAACCAAAG AGTTTTATTC CAGTATCCCA 
TCATCCTCAG TTCAGCAAAG TATACTCTGT CTATTATTTG ACCTCCATGT TGACAGTACA 
AGTTCACAGA CAGCTACACT GATCAGTAAG ACTTTAAAAG GATTACGACT TGACGCTGAA 
CAGATAATCA TTGAACTGAA CAAAGTCAAC AAATGCACTG GAGCAGCCAC TGTCAAACAA 
GCAAAGCGAG TTAAAAGGCA AGAGAAGGCA ACAGATGATG GTGATAATGT CTTTGAGAGC 
AGAGAATGGA TGCGAATTAC TCTGATTTGT GAATTTTTAC AACACAAAAA GAAAATCAAC 
AATTCTCAGC TTCTTGTTCC TGTTCTGTTT AACCTTCTGT CACAGTGCTT TGAAGCTGAG 
ACAGCTGACC AGATTTCTAC GGAATATATT AAACAGTTAA TACTGAGTTC TATACTCAAT 
ATTTGTAATA AACTTTCACC GGACAACAAA CCGTTGCCCT CAGATGTACT GACTGAAGAG 
CAGTTTAAAG TTGAGTTGAT AATTCAGTGC ATACGAGTCT CGGACAATCC ACAAACGCAT 
CACCATGCCA TGTTGCTATT GGCAACTGCT GCTGGTATAT TTCCTGAACA TGTACTTCAT 
AACATCATGT CAATCTTTAC GTTTATGGGA ACTAATCTAC TACGGCAAGA TGATTCTTAC 
AGTTCTCAAG TAATCAATAA AATTGTTGAA ACGGTAATAC CTGTCTTGAT TAAGGCAAGT 
GAGGAAGAAA TCCTAAAAGA AAAACTTGGA GGAGGGATAG ATGAGATTGT TGCCATGGTG 
ATCCAGGTAT TCGTTGATGC ATTGCCACAC ATTCCTGCTC ATCGTAAACA GCCGGTGTTT 
CATCAACTGA TTGGTACGCT TGGAGGAGAC AGATTTTTGT GGATGACTGT CGTACTGTTG 
TTGGTATGCC ATGTAGACAC ATCTACACAG AAGGATGACG TGGTTGATAA GGGTATCACT 
GGTTTGAATA CAGATATTGA ATTCTGTCTG TCAATCTGTA ATAACTGTGC ACCCCATGTG 
CAAGTCAATA ATATGATTAA AATATTGAAA TATCTTGGCA CATTGCCAGA AGACAAAGAA 
GAATCTGCAA CCCCTCACAA GAGAATCATA GGAACAAGAC GGAAATCACA ACTTAAGAAA 
ACTCATCAAT TATTTGATGT TGAGAAACAT ACAGCTAAAC AATTAAGACA GTTCAAATAT 
ACTGTGGTTA ATTTCATCCC CCATTTAATA TCTAGTTCTA CTTACATTAG TCAGGTTGCA 
GCTTTGTCAG TAGAGGAAAC AAACACTATG AAAACACTAT ATCAAAGTCT ATTAGAAGAA 
AACCTACGAT ACATTGCATG TATAGCCAGA TTGCTGGAGA AAAACCATGA CAAACCCACT 
GTGAAATTCT GGAGAGCTTT GTTACATAAA GCCTATGATG TATTGGACAA GATAAATGCA 
TTACTACCAA CACATATATT CATCAATGTG GTATCTGGTC TCATGCATAA TGACATCGCT 
GCCGTACGAC GTAAAGCTAT GGAGTTATTA AATAACAAAC TAACGCAACA TAAAGAACCG 
TTCAATGAAC AACAGGTGTC ACTTTTACTT GCGCTTGTTC CAGAACTTGT ATCAGTAACC 
AAGGCAACCA AAATAGTCGG TGGTGGTTTG TCAGAACTTG ATGTCAACCG ACAGACCGCC 
CTCTATAGTC TCAAGTTATT GTGTAAAGTG TTGGGTTGTG ATAATCCGCA ACACTTTACT 
CCAGTAATGG ATATCGTCAC TAAAGTGTTC TCAACAAAAG ACATCAACTC CCAGGTTGCT 
GCTAGTGCAC TGCTATGCTT GGCTGAGCTG TGTTCCAGTC TGAAAGCCCA TTGTATTCCT 
TATTTGAACA AATTTATGCC TTCATTGGTA ACAATTCTTG ATAATAAGGA CCATCTAAAA 
AGTCATGATT TGCTATTACT GAGTGCAGTT ACTGCGTTAC ATAAAGTTGT GGATAATCTG 
ACTCACTTCC TGAGCCCCTA TCTGTTGGAT ATTTTGACAA AGGTGTCTGC TCTGTTTGCG 
CTGGATAAAG ACGAGAAATC CCAACTAACG ATGAGACTTA AAGCAATTCA TCAGAAGTTA 
TCTTCCACTG TGTCTCCAAG AGTTCTTCTA CCTGTCATAA CACAATGTTA TCACCATGTC 
GTAGACGAAC ATGAGCTTTC AATTATACCT TTGATGTCAG TCCTTGGGGA TAGTATTTCA 
AGTATGTCTA AAGAAGATAT GACTGCTCAT CAGACGTCTC TACTCAATCT ATTCCTGTCT 
GTATTCGATT ACAGAGCATC TCATCCTCAG GTATCTATGG TTACCATTGA AGAAATTGAA 
AGAAGTTCCA TAAATGCTTT CTTGTCATTG GTTATGAAGT CATCTGAAGC AACATTCAAA 
CCAATATTTT TCAAGCTCTA TGATTGGGCT ACACAGACTG GAGCTCATAA AGAGAGGTTA 
TTGACTTTTT ACCGTCTATC TGATAACATA GCTGAAAAGC TAAAGGGATT ATTTACTCTC 
TTTGCTGGAC ATATTGTAAA AAACTGTGCT ACTTTGTTGA ATGACAACAA TTCTTCAAAA 
ACAGATTTAA AGTTTTTTGA GATCGATGGG GATGAAGATG AAGAAGCCCA GAGTGTAGAT 
GAGAAATCTA CTTTGTTGCT ACAATACATC ATTGGTTGTC TTCATAAGTG TTTTCTGTAT 
GATACAGAAG GATTTCTGAA TCGGGAGAGA TTTGAATGTT TGATGCAACC AATAGTAGAT 
CAGATTGATA ACCTACAAGG CGGTATTGAA GTCTACAAAG AAAGAATGAC ATCATATCTT 
ACACCATGTA TTGGTCAGCT GCTTGTTGCT GCTGGTGATG ATTCGTCATG GAAACCGCTT 
AATTATCAAG TTTTGTTGAA GATGAGAAGT GATTCTGCAA AGGTCCGCTT TGCTGCACTT 
ACAGTGTTAC AAGAAATTCA TAGTAAACTT GGTGAAGATT TTCAGATTCT GATTCCAGAA 
GCTATTCCAT TCTTGGCTGA ACTATTAGAA GATGAATCAG AAGAAGTTGA AAAACAGTGC 
CATCAAGTTA TAGAAGAGAT TGAGAAAACA TTTGGGGAGT CGTTACAAAA ATATTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HEATR1 cDNA ORF clone, Saccoglossus kowalevskii

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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