LOC8279846 cDNA ORF clone, Ricinus communis(castor bean)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8279846
Entrez Gene ID	8279846
Full Name	aconitate hydratase, cytoplasmic
Gene Type	protein-coding
Organism	Ricinus communis(castor bean)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015727475.2	XP_015582961.1	aconitate hydratase, cytoplasmic
XM_015727475.1	XP_015582961.1	aconitate hydratase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following LOC8279846 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8279846 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORb29301	XM_015727475.2 Latest version!	Ricinus communis aconitate hydratase, cytoplasmic (LOC8279846), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ORb29301	XM_015727475.1	Ricinus communis aconitate hydratase, cytoplasmic (LOC8279846), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8279846 ( XM_015727475.2 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_015582961.1 Ricinus communis aconitate hydratase, cytoplasmic (LOC8279846), mRNA.

Price & Availability↑

CloneID	ORb29301
Clone ID Related Accession (Same CDS sequence)	XM_015727475.2 , XM_015727475.1
Accession Version	XM_015727475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526918400000
Organism	Ricinus communis(castor bean)
Product	aconitate hydratase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994896.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 23, 2018 this sequence version replaced XM_015727475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ricinus communis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTAC CAACTTCTCC TTATTCTTCT TCTTCAGCAC TCCTCAGGTC TTCCCACTTA 
GTTTCTAGAA TCTCCTCTCT CTCACCGAAA TTCCCTTCTC CTACGTCGTT CCGTTCCCTT 
TCTCCTTCGT CGTTTCGCTA TCAGAATTCT CGTTCTTTTA GCTTCTCCTC GGCGTTACGT 
TCGTTACCTT GCGCTGTTTC ACGCTGGAGC CATAGAGTTG ATTGGCGCTC ACCGATCAGT 
CTTCGGGCTC AGGCTCCAGC GGCCTCGCCG GTAATCGAGC GAAAGCTTTC TACTATTGCA 
TCTGAACATC CTTTNNNNNN NNNNNNNNNN NNNNNNNNNN CCAAACCTGG TGGTGGAGAG 
TTTGGAAAGT TCTATAGCTT AACTGCTTTG GATGATCCCA GAATAGACAG ATTGCCATAT 
TCTATAAAGA TACTTTTGGA ATCAGCAATA CGTAATTGTG ATAACTTCCA AGTTGCAAAA 
GAACATGTGG AGAAAATTAT TGACTGGGAA AATACATCTC TCAAGCAAGT TGAAATCCCC 
TTCAAGCCTG CTCGTGTTCT TTTACAGGAT TTTACAGGAG TACCAGCAGT GGTTGACCTT 
GCTTGTATGC GCGATGCTAT AAAGAATCTT GGCAGTGATC CTAAAAAAAT CAACCCATTG 
GTTCCTGTAG ACCTTGTTGT TGATCACTCA GTTCAGGTAG ATGTTACAAG ATCTGAAAAT 
GCAGTCCAAG CAAATATGGA AAATGAATTT CAGAGGAACA AAGAGAGATT TGCTTTCCTC 
AAATGGGGTG CTTCTGCTTT CCAGAACATG CTTGTTGTTC CACCTGGATC AGGAATTGTT 
CATCAGGTTA ACTTGGAGTA TCTTGGACGT GTTGTCTTTA ACACTGATGG CCTCCTCTAT 
CCTGATAGCT TGGTTGGTAC TGACTCGCAC ACGACTATGA TTGATGGGTT AGGAGTTGCT 
GGCTGGGGTG TTGGAGGAAT TGAAGCAGAA GCAACAATGC TTGGTCAGCC TATGAGCATG 
GTCTTGCCAG GTGTTGTTGG ATTCAAATTG TCTGGAAAGT TGCGTGATGG TGTCACAGCA 
ACTGACTTGG TTTTGACTGT GACCCAAATG CTGAGAAAGC ATGGCGTTGT TGGCAAGTTT 
GTTGAGTTTT ACGGTGATGG AATGGACGAA TTGTCATTGG CTGATAGGGC CACTATTGCA 
AATATGTCTC CTGAATATGG AGCTACCATG GGCTTTTTTC CTGTGGATCA TGCTACTTTG 
CAGTACCTTA AATTAACAGG AAGGAGTGAT GAGACTGTTG CAATGATAGA AGCATACTTG 
CGCGCAAACA AAATGTTTGT CGACTACAAT GAGCCTCAAG AAGAGCAAGT GTATACTTCT 
TACTTGCAAT TGGACCTAGC AGATGTTGAA CCCTGCGTTT CAGGACCAAA GAGACCTCAT 
GATCGGGTTC CTTTGAAAGA AATGAAGGCT GACTGGCAGA AATGTCTTGA TAACCGGGCT 
GGTTTTAAGG GCTTTGGTGT GCCAAAGGAA GAACAAGATA AGGTGGCAAA GTTCTCATTC 
CATGGGCAGC CTGCTGAACT TAAACATGGA AGTGTTGTTA TTGCTGCCAT TACAAGTTGT 
ACAAACACAT CAAACCCCAG TGTTATGCTT GGGGCTGGTC TTGTTGCAAA AAAAGCTTGT 
GAACTTGGTT TGCAGGTTAA GCCATGGATT AAAACTAGTC TTGCTCCAGG TTCTGGAGTA 
GTCACAAAAT ACTTGCTCCA GAGTGGCCTG CAACGCTACT TGAATGAGCT GGGCTTTCAC 
ATTGTTGGTT ATGGTTGCAC CACTTGTATT GGGAATTCTG GAGAACTTGA TCCATCTGTT 
GCTTCTGCAA TTTCAGATAA TGACATTATT GCTGCAGCTG TGCTTTCTGG AAATCGGAAC 
TTTGAAGGCC GTGTTCATCC ACTTACAAGA GCTAACTACC TTGCTTCACC TCCATTAGTT 
GTTGCCTATG CACTGGCTGG CACGGTTAAT ATTGACTTTG AGGAGGAGCC AATTGGAACT 
AACAAGGATG GAAAGAATAT ATACTTCAAG GATGTTTGGC CTACTAACGA TGAAATAGCT 
GAGGTTGTAC AATCTAATGT CTTACCGAGC ATGTTCAAAA GCACTTATGA GGCTATCACA 
AAAGGCAATC CCATGTGGAG TCAGCTGTCT ATCCCTACTT CCGTACTTTA CTCGTGGGAT 
CCAAAATCAA CATACATCCA TGAGCCACCA TATTTCGAGG ACATGACTAT GGACCCGCCA 
GGTCCTCATG GGGTGAAGGA TGCTTACTGC TTGCTAACAT TTGGAGACAG CATAACTACT 
GATCATATTT CCCCCGCAGG CAGCATCCAT AAAGACAGCC CTGCAGCCAA ATATCTCCTT 
GAGCGTGGGG TTGAACCTAA AGACTTCAAC TCTTATGGTA GCCGTCGGGG CAATGACGAA 
GTAATGGCAA GAGGCACATT TGCCAACATT CGCCTCGTCA ACAAGCTTTT AAATGGAGAA 
GTGGGCCCAA AAACTATCCA CATTCCTACA GGAGAGAAGC TTCATGTGTT TGATGCAGCA 
ATGAGATACA AGGAAGCTGG GGAGAACACT ATTGTCCTGG CAGGAGCAGA GTATGGAAGT 
GGCAGCTCTC GAGATTGGGC TGCCAAGGGT CCAATGCTGC TGGGAGTGAA AGCAGTGATT 
GCCAAGAGCT TTGAGAGAAT TCATCGAGGT AACCTAGTTG GCATGGGTAT CATTCCTCTA 
TGTTTCAAGC CTGGTGAGGA CGCAGACACA TTAGGATTGA CGGGTCATGA GCGATACACT 
ATTGACCTTC CAGACAAGAT CAGTGAGATA AGGCCTGGAC AAGAAGTTGG TGTTAAAACT 
GACACTGGCA AATCTTTCGC TTGCAGGGTG CGCTTTGACA CGGAGGTTGA GTTGGCATAT 
TTTGATCATG GGGGCATCCT CCCATACGTC ATTAGAAACC TTAGTAAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015582961.1
CDS	168..3161
Translation	MYLPTSPYSSSSALLRSSHLVSRISSLSPKFPSPTSFRSLSPSSFRYQNSRSFSFSSALRSLPCAVSRWSHRVDWRSPISLRAQAPAASPVIERKLSTIASEHPnullnullnullnullnullnullnullnullnullnullKPGGGEFGKFYSLTALDDPRIDRLPYSIKILLESAIRNCDNFQVAKEHVEKIIDWENTSLKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAIKNLGSDPKKINPLVPVDLVVDHSVQVDVTRSENAVQANMENEFQRNKERFAFLKWGASAFQNMLVVPPGSGIVHQVNLEYLGRVVFNTDGLLYPDSLVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLSGKLRDGVTATDLVLTVTQMLRKHGVVGKFVEFYGDGMDELSLADRATIANMSPEYGATMGFFPVDHATLQYLKLTGRSDETVAMIEAYLRANKMFVDYNEPQEEQVYTSYLQLDLADVEPCVSGPKRPHDRVPLKEMKADWQKCLDNRAGFKGFGVPKEEQDKVAKFSFHGQPAELKHGSVVIAAITSCTNTSNPSVMLGAGLVAKKACELGLQVKPWIKTSLAPGSGVVTKYLLQSGLQRYLNELGFHIVGYGCTTCIGNSGELDPSVASAISDNDIIAAAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVNIDFEEEPIGTNKDGKNIYFKDVWPTNDEIAEVVQSNVLPSMFKSTYEAITKGNPMWSQLSIPTSVLYSWDPKSTYIHEPPYFEDMTMDPPGPHGVKDAYCLLTFGDSITTDHISPAGSIHKDSPAAKYLLERGVEPKDFNSYGSRRGNDEVMARGTFANIRLVNKLLNGEVGPKTIHIPTGEKLHVFDAAMRYKEAGENTIVLAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRGNLVGMGIIPLCFKPGEDADTLGLTGHERYTIDLPDKISEIRPGQEVGVKTDTGKSFACRVRFDTEVELAYFDHGGILPYVIRNLSKE

Target ORF information:

RefSeq Version	XM_015727475.2
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis aconitate hydratase, cytoplasmic (LOC8279846), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015727475.2

ORF Insert Sequence:

ATGTACTTAC CAACTTCTCC TTATTCTTCT TCTTCAGCAC TCCTCAGGTC TTCCCACTTA 
GTTTCTAGAA TCTCCTCTCT CTCACCGAAA TTCCCTTCTC CTACGTCGTT CCGTTCCCTT 
TCTCCTTCGT CGTTTCGCTA TCAGAATTCT CGTTCTTTTA GCTTCTCCTC GGCGTTACGT 
TCGTTACCTT GCGCTGTTTC ACGCTGGAGC CATAGAGTTG ATTGGCGCTC ACCGATCAGT 
CTTCGGGCTC AGGCTCCAGC GGCCTCGCCG GTAATCGAGC GAAAGCTTTC TACTATTGCA 
TCTGAACATC CTTTNNNNNN NNNNNNNNNN NNNNNNNNNN CCAAACCTGG TGGTGGAGAG 
TTTGGAAAGT TCTATAGCTT AACTGCTTTG GATGATCCCA GAATAGACAG ATTGCCATAT 
TCTATAAAGA TACTTTTGGA ATCAGCAATA CGTAATTGTG ATAACTTCCA AGTTGCAAAA 
GAACATGTGG AGAAAATTAT TGACTGGGAA AATACATCTC TCAAGCAAGT TGAAATCCCC 
TTCAAGCCTG CTCGTGTTCT TTTACAGGAT TTTACAGGAG TACCAGCAGT GGTTGACCTT 
GCTTGTATGC GCGATGCTAT AAAGAATCTT GGCAGTGATC CTAAAAAAAT CAACCCATTG 
GTTCCTGTAG ACCTTGTTGT TGATCACTCA GTTCAGGTAG ATGTTACAAG ATCTGAAAAT 
GCAGTCCAAG CAAATATGGA AAATGAATTT CAGAGGAACA AAGAGAGATT TGCTTTCCTC 
AAATGGGGTG CTTCTGCTTT CCAGAACATG CTTGTTGTTC CACCTGGATC AGGAATTGTT 
CATCAGGTTA ACTTGGAGTA TCTTGGACGT GTTGTCTTTA ACACTGATGG CCTCCTCTAT 
CCTGATAGCT TGGTTGGTAC TGACTCGCAC ACGACTATGA TTGATGGGTT AGGAGTTGCT 
GGCTGGGGTG TTGGAGGAAT TGAAGCAGAA GCAACAATGC TTGGTCAGCC TATGAGCATG 
GTCTTGCCAG GTGTTGTTGG ATTCAAATTG TCTGGAAAGT TGCGTGATGG TGTCACAGCA 
ACTGACTTGG TTTTGACTGT GACCCAAATG CTGAGAAAGC ATGGCGTTGT TGGCAAGTTT 
GTTGAGTTTT ACGGTGATGG AATGGACGAA TTGTCATTGG CTGATAGGGC CACTATTGCA 
AATATGTCTC CTGAATATGG AGCTACCATG GGCTTTTTTC CTGTGGATCA TGCTACTTTG 
CAGTACCTTA AATTAACAGG AAGGAGTGAT GAGACTGTTG CAATGATAGA AGCATACTTG 
CGCGCAAACA AAATGTTTGT CGACTACAAT GAGCCTCAAG AAGAGCAAGT GTATACTTCT 
TACTTGCAAT TGGACCTAGC AGATGTTGAA CCCTGCGTTT CAGGACCAAA GAGACCTCAT 
GATCGGGTTC CTTTGAAAGA AATGAAGGCT GACTGGCAGA AATGTCTTGA TAACCGGGCT 
GGTTTTAAGG GCTTTGGTGT GCCAAAGGAA GAACAAGATA AGGTGGCAAA GTTCTCATTC 
CATGGGCAGC CTGCTGAACT TAAACATGGA AGTGTTGTTA TTGCTGCCAT TACAAGTTGT 
ACAAACACAT CAAACCCCAG TGTTATGCTT GGGGCTGGTC TTGTTGCAAA AAAAGCTTGT 
GAACTTGGTT TGCAGGTTAA GCCATGGATT AAAACTAGTC TTGCTCCAGG TTCTGGAGTA 
GTCACAAAAT ACTTGCTCCA GAGTGGCCTG CAACGCTACT TGAATGAGCT GGGCTTTCAC 
ATTGTTGGTT ATGGTTGCAC CACTTGTATT GGGAATTCTG GAGAACTTGA TCCATCTGTT 
GCTTCTGCAA TTTCAGATAA TGACATTATT GCTGCAGCTG TGCTTTCTGG AAATCGGAAC 
TTTGAAGGCC GTGTTCATCC ACTTACAAGA GCTAACTACC TTGCTTCACC TCCATTAGTT 
GTTGCCTATG CACTGGCTGG CACGGTTAAT ATTGACTTTG AGGAGGAGCC AATTGGAACT 
AACAAGGATG GAAAGAATAT ATACTTCAAG GATGTTTGGC CTACTAACGA TGAAATAGCT 
GAGGTTGTAC AATCTAATGT CTTACCGAGC ATGTTCAAAA GCACTTATGA GGCTATCACA 
AAAGGCAATC CCATGTGGAG TCAGCTGTCT ATCCCTACTT CCGTACTTTA CTCGTGGGAT 
CCAAAATCAA CATACATCCA TGAGCCACCA TATTTCGAGG ACATGACTAT GGACCCGCCA 
GGTCCTCATG GGGTGAAGGA TGCTTACTGC TTGCTAACAT TTGGAGACAG CATAACTACT 
GATCATATTT CCCCCGCAGG CAGCATCCAT AAAGACAGCC CTGCAGCCAA ATATCTCCTT 
GAGCGTGGGG TTGAACCTAA AGACTTCAAC TCTTATGGTA GCCGTCGGGG CAATGACGAA 
GTAATGGCAA GAGGCACATT TGCCAACATT CGCCTCGTCA ACAAGCTTTT AAATGGAGAA 
GTGGGCCCAA AAACTATCCA CATTCCTACA GGAGAGAAGC TTCATGTGTT TGATGCAGCA 
ATGAGATACA AGGAAGCTGG GGAGAACACT ATTGTCCTGG CAGGAGCAGA GTATGGAAGT 
GGCAGCTCTC GAGATTGGGC TGCCAAGGGT CCAATGCTGC TGGGAGTGAA AGCAGTGATT 
GCCAAGAGCT TTGAGAGAAT TCATCGAGGT AACCTAGTTG GCATGGGTAT CATTCCTCTA 
TGTTTCAAGC CTGGTGAGGA CGCAGACACA TTAGGATTGA CGGGTCATGA GCGATACACT 
ATTGACCTTC CAGACAAGAT CAGTGAGATA AGGCCTGGAC AAGAAGTTGG TGTTAAAACT 
GACACTGGCA AATCTTTCGC TTGCAGGGTG CGCTTTGACA CGGAGGTTGA GTTGGCATAT 
TTTGATCATG GGGGCATCCT CCCATACGTC ATTAGAAACC TTAGTAAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8279846 ( XM_015727475.1 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_015582961.1 Ricinus communis aconitate hydratase, cytoplasmic (LOC8279846), mRNA.

Price & Availability↑

CloneID	ORb29301
Clone ID Related Accession (Same CDS sequence)	XM_015727475.2 , XM_015727475.1
Accession Version	XM_015727475.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1456243200000
Organism	Ricinus communis(castor bean)
Product	aconitate hydratase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994896.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ricinus communis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTAC CAACTTCTCC TTATTCTTCT TCTTCAGCAC TCCTCAGGTC TTCCCACTTA 
GTTTCTAGAA TCTCCTCTCT CTCACCGAAA TTCCCTTCTC CTACGTCGTT CCGTTCCCTT 
TCTCCTTCGT CGTTTCGCTA TCAGAATTCT CGTTCTTTTA GCTTCTCCTC GGCGTTACGT 
TCGTTACCTT GCGCTGTTTC ACGCTGGAGC CATAGAGTTG ATTGGCGCTC ACCGATCAGT 
CTTCGGGCTC AGGCTCCAGC GGCCTCGCCG GTAATCGAGC GAAAGCTTTC TACTATTGCA 
TCTGAACATC CTTTNNNNNN NNNNNNNNNN NNNNNNNNNN CCAAACCTGG TGGTGGAGAG 
TTTGGAAAGT TCTATAGCTT AACTGCTTTG GATGATCCCA GAATAGACAG ATTGCCATAT 
TCTATAAAGA TACTTTTGGA ATCAGCAATA CGTAATTGTG ATAACTTCCA AGTTGCAAAA 
GAACATGTGG AGAAAATTAT TGACTGGGAA AATACATCTC TCAAGCAAGT TGAAATCCCC 
TTCAAGCCTG CTCGTGTTCT TTTACAGGAT TTTACAGGAG TACCAGCAGT GGTTGACCTT 
GCTTGTATGC GCGATGCTAT AAAGAATCTT GGCAGTGATC CTAAAAAAAT CAACCCATTG 
GTTCCTGTAG ACCTTGTTGT TGATCACTCA GTTCAGGTAG ATGTTACAAG ATCTGAAAAT 
GCAGTCCAAG CAAATATGGA AAATGAATTT CAGAGGAACA AAGAGAGATT TGCTTTCCTC 
AAATGGGGTG CTTCTGCTTT CCAGAACATG CTTGTTGTTC CACCTGGATC AGGAATTGTT 
CATCAGGTTA ACTTGGAGTA TCTTGGACGT GTTGTCTTTA ACACTGATGG CCTCCTCTAT 
CCTGATAGCT TGGTTGGTAC TGACTCGCAC ACGACTATGA TTGATGGGTT AGGAGTTGCT 
GGCTGGGGTG TTGGAGGAAT TGAAGCAGAA GCAACAATGC TTGGTCAGCC TATGAGCATG 
GTCTTGCCAG GTGTTGTTGG ATTCAAATTG TCTGGAAAGT TGCGTGATGG TGTCACAGCA 
ACTGACTTGG TTTTGACTGT GACCCAAATG CTGAGAAAGC ATGGCGTTGT TGGCAAGTTT 
GTTGAGTTTT ACGGTGATGG AATGGACGAA TTGTCATTGG CTGATAGGGC CACTATTGCA 
AATATGTCTC CTGAATATGG AGCTACCATG GGCTTTTTTC CTGTGGATCA TGCTACTTTG 
CAGTACCTTA AATTAACAGG AAGGAGTGAT GAGACTGTTG CAATGATAGA AGCATACTTG 
CGCGCAAACA AAATGTTTGT CGACTACAAT GAGCCTCAAG AAGAGCAAGT GTATACTTCT 
TACTTGCAAT TGGACCTAGC AGATGTTGAA CCCTGCGTTT CAGGACCAAA GAGACCTCAT 
GATCGGGTTC CTTTGAAAGA AATGAAGGCT GACTGGCAGA AATGTCTTGA TAACCGGGCT 
GGTTTTAAGG GCTTTGGTGT GCCAAAGGAA GAACAAGATA AGGTGGCAAA GTTCTCATTC 
CATGGGCAGC CTGCTGAACT TAAACATGGA AGTGTTGTTA TTGCTGCCAT TACAAGTTGT 
ACAAACACAT CAAACCCCAG TGTTATGCTT GGGGCTGGTC TTGTTGCAAA AAAAGCTTGT 
GAACTTGGTT TGCAGGTTAA GCCATGGATT AAAACTAGTC TTGCTCCAGG TTCTGGAGTA 
GTCACAAAAT ACTTGCTCCA GAGTGGCCTG CAACGCTACT TGAATGAGCT GGGCTTTCAC 
ATTGTTGGTT ATGGTTGCAC CACTTGTATT GGGAATTCTG GAGAACTTGA TCCATCTGTT 
GCTTCTGCAA TTTCAGATAA TGACATTATT GCTGCAGCTG TGCTTTCTGG AAATCGGAAC 
TTTGAAGGCC GTGTTCATCC ACTTACAAGA GCTAACTACC TTGCTTCACC TCCATTAGTT 
GTTGCCTATG CACTGGCTGG CACGGTTAAT ATTGACTTTG AGGAGGAGCC AATTGGAACT 
AACAAGGATG GAAAGAATAT ATACTTCAAG GATGTTTGGC CTACTAACGA TGAAATAGCT 
GAGGTTGTAC AATCTAATGT CTTACCGAGC ATGTTCAAAA GCACTTATGA GGCTATCACA 
AAAGGCAATC CCATGTGGAG TCAGCTGTCT ATCCCTACTT CCGTACTTTA CTCGTGGGAT 
CCAAAATCAA CATACATCCA TGAGCCACCA TATTTCGAGG ACATGACTAT GGACCCGCCA 
GGTCCTCATG GGGTGAAGGA TGCTTACTGC TTGCTAACAT TTGGAGACAG CATAACTACT 
GATCATATTT CCCCCGCAGG CAGCATCCAT AAAGACAGCC CTGCAGCCAA ATATCTCCTT 
GAGCGTGGGG TTGAACCTAA AGACTTCAAC TCTTATGGTA GCCGTCGGGG CAATGACGAA 
GTAATGGCAA GAGGCACATT TGCCAACATT CGCCTCGTCA ACAAGCTTTT AAATGGAGAA 
GTGGGCCCAA AAACTATCCA CATTCCTACA GGAGAGAAGC TTCATGTGTT TGATGCAGCA 
ATGAGATACA AGGAAGCTGG GGAGAACACT ATTGTCCTGG CAGGAGCAGA GTATGGAAGT 
GGCAGCTCTC GAGATTGGGC TGCCAAGGGT CCAATGCTGC TGGGAGTGAA AGCAGTGATT 
GCCAAGAGCT TTGAGAGAAT TCATCGAGGT AACCTAGTTG GCATGGGTAT CATTCCTCTA 
TGTTTCAAGC CTGGTGAGGA CGCAGACACA TTAGGATTGA CGGGTCATGA GCGATACACT 
ATTGACCTTC CAGACAAGAT CAGTGAGATA AGGCCTGGAC AAGAAGTTGG TGTTAAAACT 
GACACTGGCA AATCTTTCGC TTGCAGGGTG CGCTTTGACA CGGAGGTTGA GTTGGCATAT 
TTTGATCATG GGGGCATCCT CCCATACGTC ATTAGAAACC TTAGTAAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015582961.1
CDS	160..3153
Translation	MYLPTSPYSSSSALLRSSHLVSRISSLSPKFPSPTSFRSLSPSSFRYQNSRSFSFSSALRSLPCAVSRWSHRVDWRSPISLRAQAPAASPVIERKLSTIASEHPnullnullnullnullnullnullnullnullnullnullKPGGGEFGKFYSLTALDDPRIDRLPYSIKILLESAIRNCDNFQVAKEHVEKIIDWENTSLKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAIKNLGSDPKKINPLVPVDLVVDHSVQVDVTRSENAVQANMENEFQRNKERFAFLKWGASAFQNMLVVPPGSGIVHQVNLEYLGRVVFNTDGLLYPDSLVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLSGKLRDGVTATDLVLTVTQMLRKHGVVGKFVEFYGDGMDELSLADRATIANMSPEYGATMGFFPVDHATLQYLKLTGRSDETVAMIEAYLRANKMFVDYNEPQEEQVYTSYLQLDLADVEPCVSGPKRPHDRVPLKEMKADWQKCLDNRAGFKGFGVPKEEQDKVAKFSFHGQPAELKHGSVVIAAITSCTNTSNPSVMLGAGLVAKKACELGLQVKPWIKTSLAPGSGVVTKYLLQSGLQRYLNELGFHIVGYGCTTCIGNSGELDPSVASAISDNDIIAAAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVNIDFEEEPIGTNKDGKNIYFKDVWPTNDEIAEVVQSNVLPSMFKSTYEAITKGNPMWSQLSIPTSVLYSWDPKSTYIHEPPYFEDMTMDPPGPHGVKDAYCLLTFGDSITTDHISPAGSIHKDSPAAKYLLERGVEPKDFNSYGSRRGNDEVMARGTFANIRLVNKLLNGEVGPKTIHIPTGEKLHVFDAAMRYKEAGENTIVLAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRGNLVGMGIIPLCFKPGEDADTLGLTGHERYTIDLPDKISEIRPGQEVGVKTDTGKSFACRVRFDTEVELAYFDHGGILPYVIRNLSKE

Target ORF information:

RefSeq Version	XM_015727475.1
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis aconitate hydratase, cytoplasmic (LOC8279846), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015727475.1

ORF Insert Sequence:

ATGTACTTAC CAACTTCTCC TTATTCTTCT TCTTCAGCAC TCCTCAGGTC TTCCCACTTA 
GTTTCTAGAA TCTCCTCTCT CTCACCGAAA TTCCCTTCTC CTACGTCGTT CCGTTCCCTT 
TCTCCTTCGT CGTTTCGCTA TCAGAATTCT CGTTCTTTTA GCTTCTCCTC GGCGTTACGT 
TCGTTACCTT GCGCTGTTTC ACGCTGGAGC CATAGAGTTG ATTGGCGCTC ACCGATCAGT 
CTTCGGGCTC AGGCTCCAGC GGCCTCGCCG GTAATCGAGC GAAAGCTTTC TACTATTGCA 
TCTGAACATC CTTTNNNNNN NNNNNNNNNN NNNNNNNNNN CCAAACCTGG TGGTGGAGAG 
TTTGGAAAGT TCTATAGCTT AACTGCTTTG GATGATCCCA GAATAGACAG ATTGCCATAT 
TCTATAAAGA TACTTTTGGA ATCAGCAATA CGTAATTGTG ATAACTTCCA AGTTGCAAAA 
GAACATGTGG AGAAAATTAT TGACTGGGAA AATACATCTC TCAAGCAAGT TGAAATCCCC 
TTCAAGCCTG CTCGTGTTCT TTTACAGGAT TTTACAGGAG TACCAGCAGT GGTTGACCTT 
GCTTGTATGC GCGATGCTAT AAAGAATCTT GGCAGTGATC CTAAAAAAAT CAACCCATTG 
GTTCCTGTAG ACCTTGTTGT TGATCACTCA GTTCAGGTAG ATGTTACAAG ATCTGAAAAT 
GCAGTCCAAG CAAATATGGA AAATGAATTT CAGAGGAACA AAGAGAGATT TGCTTTCCTC 
AAATGGGGTG CTTCTGCTTT CCAGAACATG CTTGTTGTTC CACCTGGATC AGGAATTGTT 
CATCAGGTTA ACTTGGAGTA TCTTGGACGT GTTGTCTTTA ACACTGATGG CCTCCTCTAT 
CCTGATAGCT TGGTTGGTAC TGACTCGCAC ACGACTATGA TTGATGGGTT AGGAGTTGCT 
GGCTGGGGTG TTGGAGGAAT TGAAGCAGAA GCAACAATGC TTGGTCAGCC TATGAGCATG 
GTCTTGCCAG GTGTTGTTGG ATTCAAATTG TCTGGAAAGT TGCGTGATGG TGTCACAGCA 
ACTGACTTGG TTTTGACTGT GACCCAAATG CTGAGAAAGC ATGGCGTTGT TGGCAAGTTT 
GTTGAGTTTT ACGGTGATGG AATGGACGAA TTGTCATTGG CTGATAGGGC CACTATTGCA 
AATATGTCTC CTGAATATGG AGCTACCATG GGCTTTTTTC CTGTGGATCA TGCTACTTTG 
CAGTACCTTA AATTAACAGG AAGGAGTGAT GAGACTGTTG CAATGATAGA AGCATACTTG 
CGCGCAAACA AAATGTTTGT CGACTACAAT GAGCCTCAAG AAGAGCAAGT GTATACTTCT 
TACTTGCAAT TGGACCTAGC AGATGTTGAA CCCTGCGTTT CAGGACCAAA GAGACCTCAT 
GATCGGGTTC CTTTGAAAGA AATGAAGGCT GACTGGCAGA AATGTCTTGA TAACCGGGCT 
GGTTTTAAGG GCTTTGGTGT GCCAAAGGAA GAACAAGATA AGGTGGCAAA GTTCTCATTC 
CATGGGCAGC CTGCTGAACT TAAACATGGA AGTGTTGTTA TTGCTGCCAT TACAAGTTGT 
ACAAACACAT CAAACCCCAG TGTTATGCTT GGGGCTGGTC TTGTTGCAAA AAAAGCTTGT 
GAACTTGGTT TGCAGGTTAA GCCATGGATT AAAACTAGTC TTGCTCCAGG TTCTGGAGTA 
GTCACAAAAT ACTTGCTCCA GAGTGGCCTG CAACGCTACT TGAATGAGCT GGGCTTTCAC 
ATTGTTGGTT ATGGTTGCAC CACTTGTATT GGGAATTCTG GAGAACTTGA TCCATCTGTT 
GCTTCTGCAA TTTCAGATAA TGACATTATT GCTGCAGCTG TGCTTTCTGG AAATCGGAAC 
TTTGAAGGCC GTGTTCATCC ACTTACAAGA GCTAACTACC TTGCTTCACC TCCATTAGTT 
GTTGCCTATG CACTGGCTGG CACGGTTAAT ATTGACTTTG AGGAGGAGCC AATTGGAACT 
AACAAGGATG GAAAGAATAT ATACTTCAAG GATGTTTGGC CTACTAACGA TGAAATAGCT 
GAGGTTGTAC AATCTAATGT CTTACCGAGC ATGTTCAAAA GCACTTATGA GGCTATCACA 
AAAGGCAATC CCATGTGGAG TCAGCTGTCT ATCCCTACTT CCGTACTTTA CTCGTGGGAT 
CCAAAATCAA CATACATCCA TGAGCCACCA TATTTCGAGG ACATGACTAT GGACCCGCCA 
GGTCCTCATG GGGTGAAGGA TGCTTACTGC TTGCTAACAT TTGGAGACAG CATAACTACT 
GATCATATTT CCCCCGCAGG CAGCATCCAT AAAGACAGCC CTGCAGCCAA ATATCTCCTT 
GAGCGTGGGG TTGAACCTAA AGACTTCAAC TCTTATGGTA GCCGTCGGGG CAATGACGAA 
GTAATGGCAA GAGGCACATT TGCCAACATT CGCCTCGTCA ACAAGCTTTT AAATGGAGAA 
GTGGGCCCAA AAACTATCCA CATTCCTACA GGAGAGAAGC TTCATGTGTT TGATGCAGCA 
ATGAGATACA AGGAAGCTGG GGAGAACACT ATTGTCCTGG CAGGAGCAGA GTATGGAAGT 
GGCAGCTCTC GAGATTGGGC TGCCAAGGGT CCAATGCTGC TGGGAGTGAA AGCAGTGATT 
GCCAAGAGCT TTGAGAGAAT TCATCGAGGT AACCTAGTTG GCATGGGTAT CATTCCTCTA 
TGTTTCAAGC CTGGTGAGGA CGCAGACACA TTAGGATTGA CGGGTCATGA GCGATACACT 
ATTGACCTTC CAGACAAGAT CAGTGAGATA AGGCCTGGAC AAGAAGTTGG TGTTAAAACT 
GACACTGGCA AATCTTTCGC TTGCAGGGTG CGCTTTGACA CGGAGGTTGA GTTGGCATAT 
TTTGATCATG GGGGCATCCT CCCATACGTC ATTAGAAACC TTAGTAAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8279846 cDNA ORF clone, Ricinus communis(castor bean)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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